Search Results
Results 1 - 50 of 2005
1 2 3 4 5 6 7 8 9 10 >
Kannaki T R TR a Indian Veterinary Research Institute , Bareilly , Uttar Pradesh , - - 2015
Toll-like receptors (TLRs), important components of innate immune response, play a pivotal role in early recognition of pathogen as well as in the initiation of robust and specific adaptive immune response. In the present study, the expression profile of chicken TLRs (TLR2A, TLR3, TLR4, TLR5, TLR7, TLR15, and TLR21) in ...
Yabe Tomio T Faculty of Applied Biological Sciences, Department of Applied Life Science, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan, - - 2015
In situ hybridization provides information for understanding the localization of gene expression in various tissues. The relative expression levels of mRNAs in a single cell can be sensitively visualized by this technique. Furthermore, since in situ hybridization is a histological technique, tissue structure is maintained after fixation, and it is ...
Fu Mei M College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan 610041, - - 2014
Estrogen and its receptors are essential hormones for normal reproductive function in males and females during developmental stage. To better understand the effect of estrogen receptor (ER) gene in yak (Bos grunniens), reverse transcription-polymerase chain reaction (PCR) was carried out to clone ERα and ERβ genes. Bioinformatics methods were used ...
Ju Jinfen J Department of Gynecology, Nanjing Municipal Organ Hospital, Nanjing, Jiangsu 210018, P.R. - - 2014
Toll-like receptor (TLR) signal transduction is a central component of the primary innate immune response to pathogenic challenge. TLR4, a member of the TLR family, is highly expressed in the endometrial cells of the uterus and could thus be a key link between human chronic endometritis (CE) and the immune ...
Latimer Jean J JJ Department of Pharmaceutical Sciences, College of Pharmacy, Nova Southeastern University, Fort Lauderdale, - - 2014
Nucleotide excision repair (NER) is important as a modulator of disease, especially in constitutive deficiencies, such as the cancer predisposition syndrome Xeroderma pigmentosum. We have found profound variation of NER capacity among normal individuals, between cell-types and during carcinogenesis. NER is a repair system for many types of DNA damage, ...
Zhang Fei F Department of Orthopedics, Ningbo Development Zone Center Hospital, Ningbo, 315800, - - 2014
Recent studies have shown that microRNA-451 (miR-451) was significantly decreased in osteosarcoma tissues and was identified as a tumor suppressor in other types of human cancers. However, its clinical significance and molecular mechanisms in osteosarcoma are still not well understood. MiR-451 levels are evaluated by quantitative reverse transcription-polymerase chain reaction ...
Xue Tian T Faculty of Life Science, Northwest University, Xi'an, Shaanxi 710069, P.R. - - 2014
Human aspartyl-(asparaginyl)-β-hydroxylase (HAAH) is a type 2 transmembrane protein and an α-ketoglutarate-dependent dioxygenase that can stereospecifically catalyze the post-translational hydroxylation reaction of β-carbon atoms of aspartic acid and asparagine residues present in epidermal growth factor-like domains of certain specific proteins. Humbug is a truncated isoform of aspartyl (asparaginyl) β-hydroxylase that lacks the ...
Chen Dong D Department of Pediatric Surgery, Shengjing Hospital of China Medical University, Liaoning 110004, P.R. - - 2014
Hirschsprung's disease (HSCR) is a congenital disorder characterized by an absence of enteric ganglion cells in the terminal regions of the gut during development. To date, the cause of HSCR remains unclear, although the pathogenesis of this complex disease is hypothesized to be influenced by numerous genetic and environmental factors. ...
Dastmalchi Mehran M Department of Biology, University of Western Ontario, London, ON, - - 2014
Soybean chalcone isomerase (CHI) family contains twelve members with unique evolutionary background, expression patterns and is compartmentalized to specific subcellular locations. The phenylpropanoid pathway produces a diverse array of plant natural products. A key branch-point enzyme, chalcone isomerase, catalyzes the reaction producing flavanones, the backbone for many downstream metabolites such ...
Cindrova-Davies T T Centre for Trophoblast Research, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, CB2 3EG, - - 2014
Early human placental and embryonic development occur in a physiologically low oxygen environment supported by histiotrophic secretions from endometrial glands. In this study, we compare the placental metabolomic profile of in the first, second and third trimesters to determine whether the energy demands are adequately met in the first trimester. ...
Xu Chunlan C The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 West Youyixi Road, Xi'an, Shaanxi, 710072, People's Republic of China, - - 2014
Iron and oxygen are essential substance for cellular activity in body tissues. Hypoxia-inducible factors (HIFs) can respond to available oxygen changes in the cellular environment and regulate the transcription of a series of target genes. The study was conducted to investigate the effects of iron supplementation on the expression of ...
Guzman Grace G Pathology, College of Medicine, Cancer Center, University of Illinois Hospital and Health Science System, 840 South Wood Street Room 130M/C 847, Chicago, IL, 60612, USA, - - 2014
Normal hepatocytes exhibit low-affinity hexokinase (glucokinase [HKIV]), but during oncogenesis, there is a switch from HKIV to HKII expression. The aims of this study were to compare the immunoexpression of HKII in non-dysplastic cirrhosis (NDC), liver cell change/dysplasia in cirrhosis (LCD), HCC, and normal liver control tissues, and to correlate ...
Hanigan Marie H MH Department of Cell Biology, University of Oklahoma Health Sciences Center, BMSB 553, 940 S.L.Young Blvd, Oklahoma City, OK, 73104, USA, - - 2014
Gamma-glutamyl transferase (GGT5) was discovered due to its ability to convert leukotriene C4 (LTC4, a glutathione S-conjugate) to LTD4 and may have an important role in the immune system. However, it was not known which cells express the enzyme in humans. We have developed a sensitive and specific antibody that ...
Lee Hui-Young HY Department of Internal Medicine, Kangwon National University Hospital, Kangwon National University School of Medicine, Chuncheon-si, Gangwon-do 200-722, Republic of - - 2014
MicroRNAs (miRNAs) are single‑stranded RNA species that constitute a class of non‑coding RNAs, and are emerging as key regulators of gene expression. Since each miRNA is capable of regulating multiple genes, miRNAs are attractive markers for studies of coordinated gene expression. In this study, we investigated miRNA expression profiling using ...
Signorile Pietro G PG Centro Italiano Endometriosi, Rome, - - 2014
The anti-mullerian hormone (AMH) is a homodimeric glycoprotein member of the transforming growth factor ƒÀ (TGF-ƒÀ) superfamily, and is secreted by Sertoli cells in the embryonic testes and is responsible of the regression of the mullerian duct. The physiological functions of this protein remain largely unknown, and its expression in ...
Michailidou Zoi Z Henry Wellcome Building for Molecular Physiology, University of Oxford, Roosevelt Drive, Oxford OX3 7BN The University of Edinburgh /BHF Centre for Cardiovascular Science, Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, - - 2014
Prolyl hydroxylase enzymes (PHDs) sense cellular oxygen upstream of hypoxia-inducible factor (HIF) signalling leading to HIF degradation in normoxic conditions. Here we demonstrate that adipose PHD2 inhibition plays a key role in the suppression of adipocyte lipolysis. Adipose Phd2 gene ablation in mice enhanced adiposity, with a parallel increase in ...
Hu Fen F College of Life Sciences, College of Psychology, Hebei United University, Tangshan, Hebei 063000, P.R. - - 2014
G-patch domain containing 2 (GPATC2), a human gene that is highly expressed in the testes, was implicated as a novel cancer/testis antigen. The present study investigated GPATC2 expression in a number of human cell lines and rat tissues, and its potential biological function in 293T cells. Semi‑quantitative reverse transcription-polymerase chain ...
Rosas-Cárdenas Flor de Fátima FD Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Unidad de Genómica Avanzada, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Km. 9.6 Libramiento Norte, Carretera Irapuato-León, CP 36821, Irapuato, Guanajuato, Mexico, - - 2014
miRNAs are a class of small non-coding RNAs that regulate gene expression. They are involved in the control of many developmental processes, including fruit development. The increasing amount of information on miRNAs, on their expression, abundance, and conservation between various species, provides a new opportunity to study the role of ...
Tsai Chung-Hung CH Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Oral Pathology, Chung Shan Medical University Hospital, Taichung, - - 2014
Oral submucous fibrosis (OSF) is regarded as a pre-cancerous condition with fibrosis in oral subepithelial connective tissue. Hypoxia-inducible factor (HIF)-1α regulates a wide variety of profibrogenic genes, which are closely associated with tissue fibrosis. The aim of this study was to compare HIF-1α expression in normal buccal mucosa tissues and ...
Li Xiuying X The Central Laboratory, China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. - - 2014
Quantitative polymerase chain reaction (qPCR) analysis is a commonly used method for the study of mRNA expression throughout the field of mesenchymal stem cell (MSC) research. This technology is simple and sensitive; however the results may vary significantly due to the use of various reference genes (RGs) as normalizers. Therefore, ...
Xie Jun-Pei JP Department of Gastroenterology, The Chenggong Hospital Affiliated to Xiamen University, Xiamen, Fujian 361003, P.R. - - 2014
This study aimed to investigate the expression and clinical significance of enoyl coenzyme A hydratase, short chain, 1 (ECHS1), in patients with colorectal cancer (CRC). The ECHS1 protein expression as detected by immunohistochemistry in 148 CRC specimens was evaluated and compared by clinical pathology and prognosis; 38 specimens from proximal ...
Chen Yan-Shan YS Department of Gynecology and Obstetrics, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, P.R. - - 2014
The aim of the present study was to explore the association between the expression of microRNA (miRNA)-181b and plasminogen activator inhibitor-1 (PAI-1) in the placental tissue of pregnant females with a hypertensive disorder complicating pregnancy (HDCP). Placental tissue samples were obtained from 48 patients with HDCP and 40 females with ...
Jia Cundong C Department of Medical Oncology, The Affiliated Tumor Hospital of Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. - - 2014
The aim of the present study was to investigate the expression level of TWIST1 in B-cell non-Hodgkin lymphoma (BNHL) and its association with the clinicopathological characteristics of BNHL. Expression levels of TWIST1 were analyzed in patients with BNHL (n=45) and lymphadenosis (n=21) using immunohistochemical staining and western blot analysis. In ...
Zhang Juan J Department of Obstetrics and Gynecology, Airforce General Hospital, Beijing 100142, P.R. - - 2014
This study aimed to investigate the expression of metastasis suppressor 1 (MTSS1) in cervical intraepithelial neoplasia (CIN) and malignant cervical tissues, and the role of MTSS1 in carcinogenesis. MTSS1 expression was detected by immunohistochemistry in 147 cervical tissue specimens collected from 30 healthy individuals, 30 patients with cervical CIN I, ...
Song Yu Y Department of Oncology, Zhangjiagang First People's Hospital, Suzhou, Jiangsu 215600, P.R. - - 2014
The present study aimed to observe the methylation status of the CpG islands at the human hedgehog interacting protein (HHIP) gene in gastric cancer tissues, peritumoral tissues and the AGS cell line, to analyze the association between the methylation status of the CpG islands and the tumorigenesis of gastric cancer. ...
Zhang Z W ZW Key Laboratory of Chicken Genetics and Breeding at Ministry of Agriculture, Key Laboratory of Animal Genetics, Breeding and Reproduction at Education Department of Heilongjiang Province, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, P. R. China School of Medicine, Shihezi University, Shihezi, 83200, P.R. - - 2014
Studies in mammalian species showed that Krüppel-like factor 2 (KLF2) regulates adipogenesis. However, its role in birds is unclear. The objective of the current study was to explore the expression and function of KLF2 in chicken adipogenesis. Results showed that chicken KLF2 (Gallus gallus KLF2 [gKLF2]) was greatly expressed in ...
Kim Ji Young JY Department of Dermatology, Cutaneous Biology Research Institute, Severance Hospital, Yonsei University College of Medicine, Seoul, - - 2014
Recent findings of increased cathelicidin protein and its proteolytic fragments in rosacea suggest a pathogenic role for cathelicidin in this disease. The relationship between cathelicidin and protease-activated receptor 2 (PAR-2) is therefore of interest, as PAR-2, expressed principally in keratinocytes, regulates pro-inflammatory cytokine expression in the skin. The purpose of ...
Lee Keun K Department of Biology, University of Miami, Coral Gables, FL 33146, - - 2014
At the head-trunk transition, hindbrain and spinal cord alignment to occipital and vertebral bones is crucial for coherent neural and skeletal system organization. Changes in neural or mesodermal tissue configuration arising from defects in the specification, patterning or relative axial placement of territories can severely compromise their integration and function. ...
Farahani Maryam Shahrabi MS Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, - - 2014
Since the activation of KRAS results in de novo endometriosis in mice, KRAS is regarded as a crucial gene in ectopic endometrial implantation. Recently, it has been reported that 31% of women with endometriosis have KRAS let-7 complementary binding site 6 single-nucleotide polymorphism (LCS6 SNP). This study addresses the correlation ...
Schennink Anke A A Schennink, Animal Science, University of California, Davis, Davis, United - - 2014
Prolactin (PRL), acting via the PRL receptor (PRLR), controls hundreds of biological processes across a range of species. Endocrine PRL elicits well-documented effects on target tissues such as the mammary glands and reproductive organs in addition to coordinating whole-body homeostasis during states such as lactation or adaptive responses to the ...
Marino Andreana A Department of Pharmaceutical Sciences and Health Products, University of Messina, Polo Annunziata, Messina, - - 2014
The Toll-Like Receptor 2 (TLR2) plays an active and important role in Staphylococcus aureus-induced chronic ocular inflammation. The aim of this study was to investigate the expression and function of TLR2 of corneal stromal cells in ex vivo rabbit model of S. aureus keratitis. Corneal buttons with sclera rims placed ...
Shen Xin - - 2014
Diwu Yanggan (DWYG) is a Chinese compound herbal preparation which consists of five Chinese herbs. This study investigates the preventative effects of DWYG on liver fibrosis induced by carbon tetrachloride (CCl4) and explores its possible mechanisms of action. Liver fibrosis was induced in male Wistar rats by injecting a 50% ...
Takahashi Hiromizu H National Children's Research Centre, Our Lady's Children's Hospital, Dublin, - - 2014
Retinoids are essential for fetal and lung development. Beta-carotene(BC) is the main dietary retinoid source and beta-carotene-15,15'-oxygenase-1 and 2 (Bcmo1,2) is the primary enzyme generating retinoid from BC in adult mammalian tissues. Placenta has a major role in the retinol homeostasis in fetal life: Since there is no fetal retinol ...
Zhang Ze Z Norman Bethune Health Science Center, Jilin University, 130000, Changchun, Jilin, - - 2014
High-mobility group A1 (HMGA1) has been suggested to play a significant role in tumor progression, but little is known about the accurate significance of HMGA1 in non-small cell lung cancer (NSCLC) patients. The aim of this study was to identify the role of HMGA1 in NSCLC. The expression status of ...
Fang Ming M From the Heart Institute, Division of Molecular Cardiovascular Biology, Cincinnati Children's Hospital Medical Center, OH (M.F., C.M.A., A.H., K.E.Y.); and Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis - - 2014
The Wnt/β-catenin signaling pathway has been implicated in human heart valve disease and is required for early heart valve formation in mouse and zebrafish. However, the specific functions of Wnt/β-catenin signaling activity in heart valve maturation and maintenance in adults have not been determined previously. Here, we show that Wnt/β-catenin ...
Hwang Jae Yeon JY J Hwang, Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Korea, Republic - - 2014
OCT4 encoded by POU5F1 has a crucial role of maintaining pluripotency in embryonic stem cells during early embryonic development and several OCT4 variants have been identified in mouse and human studies. The objective of the present study was to identify different variants of OCT4 and analyze their expression patterns in ...
He Feng F Molecular Immunology Laboratory, Capital Institute of Pediatrics, Beijing, - - 2014
Coxsackievirus B3 (CVB3) is trophic for cardiac tissue and is a major causative agent for viral myocarditis, where local viral replication in the heart may lead to heart failure or even death. Recent studies show that inserting microRNA target sequences into the genomes of certain viruses can eradicate these viruses ...
Larsson Emma E Department of Plant Biology, Swedish University of Agricultural - - 2014
Although it is generally accepted that auxin is important for the patterning of the female reproductive organ, the gynoecium, the flow as well as the temporal and spatial actions of auxin have been difficult to show during early gynoecial development. The primordium of the Arabidopsis (Arabidopsis thaliana) gynoecium is composed ...
Hu Wensheng W Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, - - 2014
The aim of this study was to determine the promoter methylation status of type 2 isoform of 11β-hydroxysteroid dehydrogenase (11β-HSD2) and its regulatory correlation with 11β-HSD2 gene expression in placentas of pre-eclampsia (PE) patients of Chinese Han ethnicity. The pathological features of placental tissues were studied using hematoxylin-eosin staining and ...
Grammen Carolien - - 2014
In order to reach sufficiently high tissue concentrations and thus be effective, vaginally applied anti-HIV microbicides that are active at the level of the immune cells must permeate across the cervicovaginal mucosal layer. Cellular efflux transporters, such as Pgp, BCRP and MRP-2, have been demonstrated to greatly affect drug disposition ...
Hakki Sema S SS Selcuk University, Faculty of Dentistry, Department of Periodontology, Konya, - - 2014
Aim; Cell-based therapy employing mesenchymal stem cells (MSCs) seems promising to obtain regeneration of dental tissues. A comparison of tissue sources, including periodontal ligament (PDL) versus pulp (P), could provide critical information to select an appropriate MSC population for designing predictable regenerative therapies. The purpose of this study was to ...
Barrie Elizabeth S ES Center for Pharmacogenomics, The Ohio State University - - 2014
Rationale: Dopamine β-hydroxylase (DBH) catalyzes the conversion of dopamine to norepinephrine in the CNS and peripherally. DBH variants are associated with large changes in circulating DBH and implicated in multiple disorders; yet causal relationships and tissue-specific effects remain unresolved. Objective: To characterize regulatory variants in DBH, effect on mRNA expression, ...
Takahashi Toshiaki T National Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin 12, - - 2014
Normal development of the fetal diaphragm requires muscularization of the diaphragm as well as the structural integrity of its underlying connective tissue components. Developmental mutations that inhibit the formation of extracellular matrix (ECM) have been shown to result in congenital diaphragmatic hernia (CDH). Copper (Cu) is an important element during ...
Koizumi Hiroki H Department of Otorhinolaryngology-Head and Neck Surgery, University of Occupational and Environmental Health, - - 2014
We investigated the electrical impedance of and the expressions of tight junction molecules in the cholesteatoma epithelium to provide supporting evidence for the acid lysis theory of bone resorption in middle ear cholesteatoma. Study subjects were patients with primary acquired middle ear cholesteatoma and those with non-cholesteatomatous chronic otitis media ...
Yogarajah Thaneswary T Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Penang, - - 2014
This study was conducted to determine the mRNA and protein expression levels of peroxisome proliferator‑activated receptors (PPARs) in visceral adipose tissue, as well as serum adipokine levels, in Sprague Dawley rats. The rats were fed either a normal (control rats) or excessive (experimental rats) intake of food for 8 or 16 weeks, ...
Sui Linlin - - 2014
As the interface between the mother and the developing fetus, the placenta is believed to play an important role in assisted reproductive technology (ART)-induced aberrant intrauterine and postnatal development. However, the mechanisms underlying aberrant placentation remain unclear, especially during the extra-embryonic tissue development and early stage of placental formation. Using ...
Letizia Claudio C Specialized Center of Secondary - - 2014
Context: Interplay between adipose tissue and adrenal glands has been recently suggested, without well-founded actions of locally adipose tissue surrounding the adrenal glands. Objective: We hypothesized that the local expression of leptin and adiponectin can be associated with pathological changes of the adrenal glands. Patients and main outcome measures: we ...
Yang Chao-Chun CC Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; International Research Center of Wound Repair and Regeneration, National Cheng Kung University, Tainan, - - 2014
Adipose tissue encircles the lower portion of anagen hair follicles and may regulate hair cycle progression. Since leptin is a major adipokine, its level of expression from the dermal white adipose tissue during hair cycle progression was studied. The result shows that leptin level is differentially expressed during hair cycle, ...
Chen Yu Y Department of Medicine and Research Center Montreal Heart Institute and Université de Montréal; Montreal, Quebec, Canada; Department of Pharmacology and Therapeutics McGill University, Montreal, Quebec, - - 2014
Heart failure (HF) causes left-atrial (LA) and left-ventricular (LV) remodeling, with particularly-prominent changes in LA that create a substrate for atrial fibrillation (AF). MicroRNAs (miRs) are potential regulators in cardiac remodeling. This study evaluated time-dependent miR expression-changes in LA and LV tissue, fibroblasts and cardiomyocytes in experimental HF. HF was ...
Kern Philip A PA Department of Medicine (P.A.K., B.S.F., B.Z.), Division of Endocrinology, and the Barnstable Brown Diabetes and Obesity Center, University of Kentucky, Lexington, Kentucky 40536; Division of Endocrinology (N.R.), University of Colorado Denver, Denver, Colorado 80220; Eastern Colorado Veterans Health Care System (N.R.), Denver, Colorado 80220; Department of Pediatrics (R.E.M.), University of Arkansas for Medical Sciences, Little Rock, Arkansas; College of Public Health (P.M.W.) and College of Health Sciences (E.E.D.-V.), University of Kentucky, Lexington, Kentucky - - 2014
Context: Although brown adipose tissue (BAT) activity is increased by a cold environment, little is known of the response of human white adipose tissue (WAT) to the cold. Design: We examined both abdominal and thigh sc WAT from 71 subjects who were biopsied in the summer or winter, and adipose ...
1 2 3 4 5 6 7 8 9 10 >