Search Results
Results 1 - 50 of 1831
1 2 3 4 5 6 7 8 9 10 >
Chalupa Allison A Department of Dermatology, University of Cincinnati College of Medicine, Cincinnati, OH, - - 2014
Galectin-3 is a family member of the carbohydrate binding proteins widely expressed by many cell types and exhibits multiple cellular functions. We demonstrate that melanocytes express galectin-3, which is predominantly localized to the cell body peripherally along the Golgi zone. Downregulation of galectin-3 in human melanocytes using shRNA technology resulted ...
Satoh Tetsurou T Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi, Japan. Electronic address: - - 2014
Emerging evidence has indicated that the transcription and processing of precursor mRNA (pre-mRNA) are functionally coupled to modulate gene expression. In collaboration with coregulators, several steroid hormone receptors have previously been shown to directly affect alternative pre-mRNA splicing coupled to hormone-induced gene transcription; however, the roles of the thyroid hormone ...
Bounedjah Ouissame O Institut National de la Santé et de la Recherche Médicale (INSERM), UMR829; Université Evry-Val d'Essonne, Evry 91025, - - 2014
The sequence of events leading to stress granule assembly in stressed cells remains elusive. We show here, using isotope labeling and ion microprobe, that proportionally more RNA than proteins are present in stress granules than in surrounding cytoplasm. We further demonstrate that the delivery of single strand polynucleotides, mRNA and ...
Zhao Yunying Y The National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China; The State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, - - 2014
ESCRT components function to form multivesicular bodies for sorting of proteins destined to the yeast vacuole. The calcium hypersensitivity of ESCRT mutants is mainly due to repressed expression of PMR1 through the Rim101/Nrg1 pathway in budding yeast. Here, we show that overexpression of PMC1 and its negative regulator gene NYV1 ...
Torres-Andrade Rodrigo R Centro de Estudios Científicos, Valdivia, Chile Universidad Austral de Chile, Valdivia, Chile Universidad de Chile, Santiago, - - 2014
Methyl CpG binding protein-2 (MECP2) is a chromatin-remodelling factor with a dual role in gene expression. Evidence from patients carrying MECP2 mutations and from transgenic mouse models demonstrates that this protein is involved in the control of body weight. However, the mechanism for this has not been fully elucidated. To ...
Zhou Dongjie - - 2014
Dimethyl sulfoxide (DMSO) is used extensively as a permeable cryoprotectant and is a common solvent utilized for several water-insoluble substances. DMSO has various biological and pharmacological activities; however, the effect of DMSO on mouse oocyte meiotic maturation remains unknown. In DMSO-treated oocytes, we observed abnormal MII oocytes that contained large ...
Adjibade Pauline P Centre de recherche du CHU de Québec, Département de biologie moléculaire, biochimie médicale et pathologie, Université Laval, Québec, PQ, - - 2014
The control of mRNA turnover is essential for the cell to rationalize its mRNA content both under physiological conditions and upon stress. Several mechanisms involved in the control of mRNA turnover have been elucidated. These include surveillance mechanisms such as nonsense-mediated decay, non-stop mediated decay and non-go-mediated decay that eliminate ...
Trejo-Muñoz Lucero L Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, Distrito Federal, - - 2014
During the early stages of development, the olfactory system plays a vital role in the survival of altricial mammals. One remarkable example is the Oryctolagus cuniculus, whose mother-young interaction greatly depends on the 2-methylbut-2-enal (2MB2) pheromone that triggers the nipple search and grasping behaviors. Olfactory stimulation with 2MB2 regulates the ...
Sontakke Sadanand S S Sontakke, Roslin institute, University of Edinburgh, Edinburgh, United - - 2014
Several different miRNAs have been proposed to regulate ovarian follicle function, however, very limited information exists on the spatiotemporal patterns of miRNA expression during follicle development. The objective of this study was to identify, using microarray, miRNA profiles associated with growth and regression of dominant-size follicles in the bovine monovular ...
Park Jong-Lyul JL Medical Genomics Research Center, KRIBB, 125 Gwahang-no, Yuseong-gu, Daejeon; Department of Functional Genomics, University of Science of Technology, 217 Gajungro, Yuseong-gu, - - 2014
MicroRNAs (miRNA) are a class of small (∼22 nucleotides) non-coding RNAs that regulate diverse biological processes at the post-transcriptional level. MiRNAs have great potential for forensic body fluid identification because they are expressed in a tissue specific manner and are less prone to degradation. Previous studies reported several miRNAs as ...
Rouhana Labib L Department of Biological Sciences, Wright State University, 3640 Colonel Glenn Highway, Dayton, OH 45435, USA Howard Hughes Medical Institute, Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, 601 S. Goodwin Ave., Urbana, IL 61801, USA - - 2014
The well-known regenerative abilities of planarian flatworms are attributed to a population of adult stem cells called neoblasts that proliferate and differentiate to produce all cell types. A characteristic feature of neoblasts is the presence of large cytoplasmic ribonucleoprotein granules named chromatoid bodies, the function of which has remained largely ...
Song Rui R 1] Division of Cellular and Developmental Biology, MCB Department, University of California at Berkeley, Berkeley, California 94705, USA - - 2014
The mir-34/449 family consists of six homologous miRNAs at three genomic loci. Redundancy of miR-34/449 miRNAs and their dominant expression in multiciliated epithelia suggest a functional significance in ciliogenesis. Here we report that mice deficient for all miR-34/449 miRNAs exhibited postnatal mortality, infertility and strong respiratory dysfunction caused by defective ...
McLean Jesse R JR Neuroregeneration Research Institute, McLean Hospital/Harvard Medical School, Belmont, MA, - - 2014
Intracellular proteinaceous inclusions are well-documented hallmarks of the fatal motor neuron disorder amyotrophic lateral sclerosis (ALS). The pathological significance of these inclusions remains unknown. Peripherin, a type III intermediate filament protein, is upregulated in ALS and identified as a component within different types of ALS inclusions. The formation of these ...
Ling Y H YH School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong - - 2014
Coiled-coil alpha-helical rod protein 1 (CCHCR1) is suggested as a candidate biomarker for psoriasis for more than a decade but its function remains poorly understood because of the inconsistent findings in the literature. CCHCR1 protein is suggested to be localized in the cytoplasm, nucleus, mitochondria, or centrosome and to regulate ...
Chen Chen C College of Aminal Science & Technology, Sichuan Agricultural University, Yaan, 625014, - - 2014
Toll-like receptors (TLRs) are vital for innate immunity, and they were expressed in various immune cells, tissues and organs. Moreover, TLRs specific expression pattern in different cells, tissue and organs have been confirmed to have correlation with the ability to resistance to pathogenic challenges. The present study aimed to determine ...
Buchan J Ross JR Department of Molecular and Cellular Biology; University of Arizona; Tucson, AZ - - 2014
Messenger ribonucleoprotein (mRNP) granules are dynamic, self-assembling structures that harbor non-translating mRNAs bound by various proteins that regulate mRNA translation, localization, and turnover. Their importance in gene expression regulation is far reaching, ranging from precise spatial-temporal control of mRNAs that drive developmental programs in oocytes and embryos, to similarly exquisite ...
Mirth Christen Kerry CK Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA - - 2014
The role of juvenile hormone (JH) in regulating the timing and nature of insect molts is well-established. Increasing evidence suggests that JH is also involved in regulating final insect size. Here we elucidate the developmental mechanism through which JH regulates body size in developing Drosophila larvae by genetically ablating the ...
Veldhoen Nik N Department of Biochemistry and Microbiology, University of Victoria, P.O. Box 3055, STN CSC, Victoria, BC, Canada V8W 3P6. Electronic address: - - 2014
With increases in active mining and continued discharge associated with former mine operations, evaluating the health of watersheds in the Canadian Yukon Territory is warranted. Current environmental assessment approaches often employ guidelines established using sentinel species not relevant to Arctic monitoring programs. The present study focused on the successful development ...
Moschner Katharina K University of Osnabrueck, - - 2014
The neuronal microtubule-associated protein tau is expressed in different variants and changes in tau isoform composition occur during development and disease. Here, we investigate a potential role of the multivalent tau mRNA-binding proteins G3BP1 and IMP1 in regulating neuronal tau expression. We demonstrate that G3BP1 and IMP1 expression induces the ...
de Mateo Sara S Center for Epigenetics and Metabolism School of Medicine, Department of Biological Chemistry University of California, - - 2014
The spermatogenic process relays in highly regulated gene expression mechanisms at the transcriptional and post-transcriptional levels to generate the male gamete that is needed for the perpetuation of the species. Small non-coding RNA pathways have been determined to participate in the post-transcriptional regulatory processes of germ cells. The most important ...
Feng Eryan E School of Life Science, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticide, 400030, - - 2014
The 14-3-3 proteins, a highly evolutionarily conserved and ubiquitous protein family in eukaryotic cells, have a range of biological functions including regulation of signal transduction, stress response, apoptosis, and control of the cell cycle. To investigate the function of 14-3-3 in Spodoptera litura, the full length of 14-3-3ζ was cloned ...
Cherry Ahmed Adnan AA The Department of Chemistry and Biochemistry, University of Windsor, Windsor, Ontario N9B3P4, - - 2014
Toxoplasma gondii is an obligate intracellular protozoan which infects one-third of the human population. Due to its high infection prevalence, Toxoplasma offers an ideal system for the study of host-parasite interaction. Similar to other eukaryotes, Toxoplasma maintains levels and localization of cytoplasmic mRNAs throughout its life cycle as part of ...
Sussman Caroline R CR Nephrology and Hypertension, Mayo Clinic College of Medicine, Rochester, - - 2014
Substantial evidence indicates the importance of elevated cAMP in polycystic kidney disease (PKD). Accumulation of cAMP in cystic tissues may be, in part, caused by enhanced adenylyl cyclase activity, but inhibition of cAMP degradation by phosphodiesterases (PDE) likely has an important role, because cAMP is inactivated much faster than it ...
Ma D M DM Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture of the People's Republic of China, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, - - 2014
Growth hormone releasing hormone (GHRH) regulates the secretion of growth hormone (GH) in the pituitary gland. A 66-bp deletion (c.-923_-858del) was detected in the 5'-flanking sequence of the largemouth bass (Micropterus salmoides) GHRH gene. In two cultured random populations of adult individuals (A: n = 170 and B: n = 150), the genotype ratios ...
Yang Linghai L Department of Pharmacology, University of Washington, Seattle, Washington - - 2014
Striatal medium spiny neurons (MSNs) mediate many of the physiological effects of dopamine, including the regulation of feeding and motor behaviors. Dopaminergic inputs from the midbrain modulate MSN excitability through pathways that involve cAMP and protein kinase A (PKA), but the physiological role of specific PKA isoforms in MSN neurons ...
Jønson Lars L Center for Genomic Medicine, Rigshospitalet, University of Copenhagen, 2100 Copenhagen, - - 2014
The IMP3 RNA-binding protein is associated with metastasis and poor outcome in human cancer. Using solid cancer transcriptome data, we found that IMP3 correlates with HMGA2 mRNA expression. Cytoplasmic IMP3 granules contain HMGA2, and IMP3 dose-dependently increases HMGA2 mRNA. HMGA2 is regulated by let-7, and let-7 antagomiRs make HMGA2 refractory ...
Sudo Ryusuke R Department of Marine Bioscience, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, - - 2014
To clarify the role of thyroid function during metamorphosis from leptocephalus to glass eel in the Japanese eel, we examined the histology of the thyroid gland and measured whole-body concentrations of thyroid hormones, thyroxine (T4) and triiodothyronine (T3), and thyroid stimulating hormone β-subunit TSH (TSHβ) mRNA expression levels in five ...
Hayez Aurélie A Cell and Tissue Laboratory-URPhyM-NARILIS, University of Namur, Namur, - - 2014
TMEM45A (DERP7, DNAPTP4 or FLJ10134) gene, belonging to the TMEM family encoding predicted transmembrane proteins, is highly expressed in epidermal keratinocytes. To investigate the potential involvement of TMEM45A during the differentiation and keratinization processes, its expression has been characterized in normal human keratinocytes and the protein subcellular localization has been ...
Shirayama Masaki M Program in Molecular Medicine, University of Massachusetts Medical School, 368 Plantation Street, Worcester, MA 01605, USA; RNA Therapeutics Institute, University of Massachusetts Medical School, 368 Plantation Street, Worcester, MA 01605, USA; Howard Hughes Medical Institute, University of Massachusetts Medical School, 368 Plantation Street, Worcester, MA 01605, - - 2014
Argonaute (AGO) proteins are key nuclease effectors of RNAi [1]. Although purified AGOs can mediate a single round of target RNA cleavage in vitro, accessory factors are required for small interfering RNA (siRNA) loading and to achieve multiple-target turnover [2, 3]. To identify AGO cofactors, we immunoprecipitated the C. elegans AGO WAGO-1, ...
Yang Huan H Stowers Institute for Medical Research, Kansas City, MO 64110, USA; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, - - 2014
RNAi is a potent mechanism for downregulating gene expression. Conserved RNAi pathway components are found in animals, plants, fungi, and other eukaryotes [1-3]. In C. elegans, the RNAi response is greatly amplified by the synthesis of abundant secondary small interfering RNAs (siRNAs) [4-6]. Exogenous double-stranded RNA is processed by Dicer and ...
Steinke John W JW Carter Immunology Center Asthma and Allergic Disease Center Departments of Medicine, University of Virginia Health Systems, Charlottesville, VA 22908-1355, - - 2014
Studies demonstrate the existence of novel receptors for cysteinyl leukotrienes (CysLTs) that are responsive to leukotriene (LT) E4 and might be pathogenic in asthma. Given the eosinophilic infiltration in this disorder, we investigated eosinophil expression of P2Y12 and gpr99 and their capacity to respond to LTE4. Receptor transcript expression was ...
Heck Melanie Vanessa MV Experimental Neurology, Department of Neurology, Goethe University Medical School, Building 89, 3rd floor, Theodor Stern Kai 7, 60590, Frankfurt am Main, - - 2014
During cell stress, the transcription and translation of immediate early genes are prioritized, while most other messenger RNAs (mRNAs) are stored away in stress granules or degraded in processing bodies (P-bodies). TIA-1 is an mRNA-binding protein that needs to translocate from the nucleus to seed the formation of stress granules ...
Jiao Ze-Xu ZX Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, IL, 60611, USA, - - 2014
To confirm that aneuploidy candidate genes are detectable in the first polar body (PB1) of MII oocytes and to investigate the age-dependent molecular changes in PB1. Aged (12-to 15-mo-old) and young (2-mo-old) mice were administered pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotrophin (hCG). MII oocytes were obtained and ...
Abrisqueta Marc M Institut de Biologia Evolutiva (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, - - 2014
Female reproductive processes, which comprise, amongst others, the synthesis of yolk proteins and the endocrine mechanisms which regulate this synthesis, need a considerable amount of energy and resources. The role of communicating that the required nutritional status has been attained is carried out by nutritional signalling pathways and, in particular, ...
Li Yibo Y National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, - - 2014
Grain chalkiness is a highly undesirable quality trait in the marketing and consumption of rice grain. However, the molecular basis of this trait is poorly understood. Here we show that a major quantitative trait locus (QTL), Chalk5, influences grain chalkiness, which also affects head rice yield and many other quality ...
Smemo Scott S 1] Department of Human Genetics, University of Chicago, Chicago, Illinois 60637, USA - - 2014
Genome-wide association studies (GWAS) have reproducibly associated variants within introns of FTO with increased risk for obesity and type 2 diabetes (T2D). Although the molecular mechanisms linking these noncoding variants with obesity are not immediately obvious, subsequent studies in mice demonstrated that FTO expression levels influence body mass and composition ...
Kong Stephanie M Y SM Diabetes and Obesity Program, Garvan Institute of Medical Research, Darlinghurst, New South Wales, 2010, - - 2014
αSynuclein plays a central causative role in Parkinson's disease (PD). Increased expression of the P-type ATPase ion pump PARK9 / ATP13A2 suppresses αSynuclein toxicity in primary neurons. Our data indicates ATP13A2 encodes a zinc pump; neurospheres from a compound heterozygous ATP13A2(-/-) patient and ATP13A2 knockdown cells are sensitive to zinc, ...
De Biasio Filomena F Università della Basilicata, Dipartimento di Scienze, via dell'Ateneo Lucano 10, 85100 Potenza, - - 2014
Odorant-binding proteins (OBPs) are soluble proteins mediating chemoreception in insects. In previous research, we investigated the molecular mechanisms adopted by aphids to detect the alarm pheromone (E)-β-farnesene and we found that the recognition of this and structurally related molecules is mediated by OBP3 and OBP7. Here we show the differential ...
Asi Yasmine T YT Queen Square Brain Bank, Department of Molecular Neuroscience, UCL Institute of Neurology, London, United - - 2014
Multiple system atrophy (MSA) is a progressive neurodegenerative disease presenting clinically with parkinsonian, cerebellar, and autonomic features. α-Synuclein (αsyn), encoded by the gene SNCA, is the main constituent of glial cytoplasmic inclusion (GCI) found in oligodendrocytes in MSA, but the methods of its accumulation have not been established. The aim ...
Wen Hong H 1] Department of Biochemistry and Molecular Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA [2] Center for Cancer Epigenetics, Center for Genetics and Genomics, and Center for Stem Cell and Developmental Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA - - 2014
Recognition of modified histones by 'reader' proteins plays a critical role in the regulation of chromatin. H3K36 trimethylation (H3K36me3) is deposited onto the nucleosomes in the transcribed regions after RNA polymerase II elongation. In yeast, this mark in turn recruits epigenetic regulators to reset the chromatin to a relatively repressive ...
Williams Dustin R DR The Department of Molecular Genetics, The Ohio State University, 105 Biological Sciences Building, 484 West 12th Avenue, Columbus, OH 43210, - - 2014
The segmental structure of the axial skeleton is formed during somitogenesis. During this process, paired somites bud from the presomitic mesoderm (PSM), in a process regulated by a genetic clock called the segmentation clock. The Notch pathway and the Notch modulator Lunatic fringe (Lfng) play multiple roles during segmentation. Lfng ...
Meikar Oliver - - 2014
The genome of male germ cells is actively transcribed during spermatogenesis to produce phase-specific protein-coding mRNAs and a considerable amount of different noncoding RNAs. Ribonucleoprotein (RNP) granule-mediated RNA regulation provides a powerful means to secure the quality and correct expression of the requisite transcripts. Haploid spermatids are characterized by a ...
Pandurangan Ak A Department of Biochemistry, Cell Biology, University of Madras, Chennai, Tamil Nadu, - - 2014
The present investigation deals with the antimetastatic role of luteolin (LUT) by inhibiting matrix metalloproteinase (MMP)-9 and -2 in azoxymethane (AOM)-induced colon carcinogenesis in Balb/C mice. Animals received AOM at a dosage of 15 mg/kg body weight intraperitoneally once a week for 3 weeks. AOM-induced mice was treated with LUT ...
Cho Sunghee S School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, - - 2014
Spinal muscular atrophy (SMA) is an autosomal recessive genetic disease, which causes death of motor neurons in the anterior horn of spinal cord. Genetic cause of SMA is the deletion or mutation of SMN1 gene, which encodes the SMN protein. Although SMA patients include SMN2 gene, a duplicate of SMN1 ...
Shatsky Ivan N IN Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University , Moscow , Russia - - 2014
Abstract The conventional paradigm of translation initiation in eukaryotes states that the cap-binding protein complex eIF4F (consisting of eIF4E, eIF4G and eIF4A) plays a central role in the recruitment of capped mRNAs to ribosomes. However, a growing body of evidence indicates that this paradigm should be revised. This review summarizes ...
Nohara Kazunari K Division of Endocrinology, Metabolism and Molecular Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA; Comprehensive Center on Obesity, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA; Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, - - 2014
Androgen excess in women is associated with visceral adiposity. However, little is known on the mechanism through which androgen promotes visceral fat accumulation. To address this issue, female mice to chronic androgen excess using 5α-dihydrotestosterone (DHT) and studied the regulation of energy homeostasis was exposed. DHT induced a leptin failure ...
Khan Usman A UA School of Medical Science, Griffith University, Gold Coast, QLD, 4215, Australia; School of Dentistry and Oral Health, Griffith University, Gold Coast, QLD, 4215, - - 2014
Ostoeclasts and foreign body giant cells (FBGCs) are both derived from the fusion of macropahges. These cells are seen in close proximity during foreign body reactions, therefore it was assumed that they might interact with each other. The aim was to identify important genes that are expressed by osteoclasts and ...
Chen H H Genetic Engineering Research Centre, College of Life Science, Chongqing University, Chongqing, China; Institute of Plant Physiology and Ecology, Chinese Academy of Sciences Key Laboratory of Insect Developmental and Evolutionary Biology, CAS, Shanghai, - - 2014
Cysteine proteinases (Cyps) play vital roles in many biological processes, including physiological and pathological reactions. In the present study, we cloned a full cDNA of SlCyp, encoding a 344-amino-acid protein from Spodoptera litura. The putative amino acid sequence shared >75% identity with Cyps from other insects. A phylogenetic analysis revealed ...
Caruso Michael A MA Department of Biological Sciences, North Dakota State University, Fargo, ND 58105 - - 2014
We used rainbow trout as a model to study the regulation of the multiple and distinct insulin (INS) and insulin receptor (IR) mRNAs by somatostatin (SS). Implantation of SS reduced growth of animals without affecting food intake. SS decreased INS1 and INS2 expression in Brockmann bodies, but increased INS1 and ...
Al Rayyan Numan N Department of Human Nutrition, Foods and Exercise, 1981 Kraft Drive, ILSB 1020 (0913), Virginia Tech, Blacksburg, VA 24061; Current Position, School of Medicine, Department of Pharmacology and Toxicology, 505 South Hancock Street, Clinical and Translational Research Building, University of Louisville, Louisville, KY - - 2014
Mice with a deletion of the hypothalamic basic helix-loop-helix transcription factor Nhlh2 display adult onset obesity. We have previously shown that Nhlh2 expression is induced by leptin. In this study, we identify a small proximal leptin-responsive promoter region in the Nhlh2 gene. This 163 bp promoter contains five putative binding ...
1 2 3 4 5 6 7 8 9 10 >