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Cherry Ahmed Adnan AA The Department of Chemistry and Biochemistry, University of Windsor, Windsor, Ontario N9B3P4, - - 2014
Toxoplasma gondii is an obligate intracellular protozoan which infects one-third of the human population. Due to its high infection prevalence, Toxoplasma offers an ideal system for the study of host-parasite interaction. Similar to other eukaryotes, Toxoplasma maintains levels and localization of cytoplasmic mRNAs throughout its life cycle as part of ...
Sussman Caroline R CR Nephrology and Hypertension, Mayo Clinic College of Medicine, Rochester, - - 2014
Substantial evidence indicates the importance of elevated cAMP in polycystic kidney disease (PKD). Accumulation of cAMP in cystic tissues may be, in part, caused by enhanced adenylyl cyclase activity, but inhibition of cAMP degradation by phosphodiesterases (PDE) likely has an important role, because cAMP is inactivated much faster than it ...
Ma D M DM Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture of the People's Republic of China, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, - - 2014
Growth hormone releasing hormone (GHRH) regulates the secretion of growth hormone (GH) in the pituitary gland. A 66-bp deletion (c.-923_-858del) was detected in the 5'-flanking sequence of the largemouth bass (Micropterus salmoides) GHRH gene. In two cultured random populations of adult individuals (A: n = 170 and B: n = 150), the genotype ratios ...
Yang Linghai L Department of Pharmacology, University of Washington, Seattle, Washington - - 2014
Striatal medium spiny neurons (MSNs) mediate many of the physiological effects of dopamine, including the regulation of feeding and motor behaviors. Dopaminergic inputs from the midbrain modulate MSN excitability through pathways that involve cAMP and protein kinase A (PKA), but the physiological role of specific PKA isoforms in MSN neurons ...
Jønson Lars L Center for Genomic Medicine, Rigshospitalet, University of Copenhagen, 2100 Copenhagen, - - 2014
The IMP3 RNA-binding protein is associated with metastasis and poor outcome in human cancer. Using solid cancer transcriptome data, we found that IMP3 correlates with HMGA2 mRNA expression. Cytoplasmic IMP3 granules contain HMGA2, and IMP3 dose-dependently increases HMGA2 mRNA. HMGA2 is regulated by let-7, and let-7 antagomiRs make HMGA2 refractory ...
Sudo Ryusuke R Department of Marine Bioscience, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, - - 2014
To clarify the role of thyroid function during metamorphosis from leptocephalus to glass eel in the Japanese eel, we examined the histology of the thyroid gland and measured whole-body concentrations of thyroid hormones, thyroxine (T4) and triiodothyronine (T3), and thyroid stimulating hormone β-subunit TSH (TSHβ) mRNA expression levels in five ...
Hayez Aurélie A Cell and Tissue Laboratory-URPhyM-NARILIS, University of Namur, Namur, - - 2014
TMEM45A (DERP7, DNAPTP4 or FLJ10134) gene, belonging to the TMEM family encoding predicted transmembrane proteins, is highly expressed in epidermal keratinocytes. To investigate the potential involvement of TMEM45A during the differentiation and keratinization processes, its expression has been characterized in normal human keratinocytes and the protein subcellular localization has been ...
Shirayama Masaki M Program in Molecular Medicine, University of Massachusetts Medical School, 368 Plantation Street, Worcester, MA 01605, USA; RNA Therapeutics Institute, University of Massachusetts Medical School, 368 Plantation Street, Worcester, MA 01605, USA; Howard Hughes Medical Institute, University of Massachusetts Medical School, 368 Plantation Street, Worcester, MA 01605, - - 2014
Argonaute (AGO) proteins are key nuclease effectors of RNAi [1]. Although purified AGOs can mediate a single round of target RNA cleavage in vitro, accessory factors are required for small interfering RNA (siRNA) loading and to achieve multiple-target turnover [2, 3]. To identify AGO cofactors, we immunoprecipitated the C. elegans AGO WAGO-1, ...
Yang Huan H Stowers Institute for Medical Research, Kansas City, MO 64110, USA; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, - - 2014
RNAi is a potent mechanism for downregulating gene expression. Conserved RNAi pathway components are found in animals, plants, fungi, and other eukaryotes [1-3]. In C. elegans, the RNAi response is greatly amplified by the synthesis of abundant secondary small interfering RNAs (siRNAs) [4-6]. Exogenous double-stranded RNA is processed by Dicer and ...
Heck Melanie Vanessa MV Experimental Neurology, Department of Neurology, Goethe University Medical School, Building 89, 3rd floor, Theodor Stern Kai 7, 60590, Frankfurt am Main, - - 2014
During cell stress, the transcription and translation of immediate early genes are prioritized, while most other messenger RNAs (mRNAs) are stored away in stress granules or degraded in processing bodies (P-bodies). TIA-1 is an mRNA-binding protein that needs to translocate from the nucleus to seed the formation of stress granules ...
Jiao Ze-Xu ZX Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, IL, 60611, USA, - - 2014
To confirm that aneuploidy candidate genes are detectable in the first polar body (PB1) of MII oocytes and to investigate the age-dependent molecular changes in PB1. Aged (12-to 15-mo-old) and young (2-mo-old) mice were administered pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotrophin (hCG). MII oocytes were obtained and ...
Abrisqueta Marc M Institut de Biologia Evolutiva (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, - - 2014
Female reproductive processes, which comprise, amongst others, the synthesis of yolk proteins and the endocrine mechanisms which regulate this synthesis, need a considerable amount of energy and resources. The role of communicating that the required nutritional status has been attained is carried out by nutritional signalling pathways and, in particular, ...
Li Yibo Y National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, - - 2014
Grain chalkiness is a highly undesirable quality trait in the marketing and consumption of rice grain. However, the molecular basis of this trait is poorly understood. Here we show that a major quantitative trait locus (QTL), Chalk5, influences grain chalkiness, which also affects head rice yield and many other quality ...
Smemo Scott S 1] Department of Human Genetics, University of Chicago, Chicago, Illinois 60637, USA - - 2014
Genome-wide association studies (GWAS) have reproducibly associated variants within introns of FTO with increased risk for obesity and type 2 diabetes (T2D). Although the molecular mechanisms linking these noncoding variants with obesity are not immediately obvious, subsequent studies in mice demonstrated that FTO expression levels influence body mass and composition ...
Kong Stephanie M Y SM Diabetes and Obesity Program, Garvan Institute of Medical Research, Darlinghurst, New South Wales, 2010, - - 2014
αSynuclein plays a central causative role in Parkinson's disease (PD). Increased expression of the P-type ATPase ion pump PARK9 / ATP13A2 suppresses αSynuclein toxicity in primary neurons. Our data indicates ATP13A2 encodes a zinc pump; neurospheres from a compound heterozygous ATP13A2(-/-) patient and ATP13A2 knockdown cells are sensitive to zinc, ...
De Biasio Filomena F Università della Basilicata, Dipartimento di Scienze, via dell'Ateneo Lucano 10, 85100 Potenza, - - 2014
Odorant-binding proteins (OBPs) are soluble proteins mediating chemoreception in insects. In previous research, we investigated the molecular mechanisms adopted by aphids to detect the alarm pheromone (E)-β-farnesene and we found that the recognition of this and structurally related molecules is mediated by OBP3 and OBP7. Here we show the differential ...
Asi Yasmine T YT Queen Square Brain Bank, Department of Molecular Neuroscience, UCL Institute of Neurology, London, United - - 2014
Multiple system atrophy (MSA) is a progressive neurodegenerative disease presenting clinically with parkinsonian, cerebellar, and autonomic features. α-Synuclein (αsyn), encoded by the gene SNCA, is the main constituent of glial cytoplasmic inclusion (GCI) found in oligodendrocytes in MSA, but the methods of its accumulation have not been established. The aim ...
Wen Hong H 1] Department of Biochemistry and Molecular Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA [2] Center for Cancer Epigenetics, Center for Genetics and Genomics, and Center for Stem Cell and Developmental Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA - - 2014
Recognition of modified histones by 'reader' proteins plays a critical role in the regulation of chromatin. H3K36 trimethylation (H3K36me3) is deposited onto the nucleosomes in the transcribed regions after RNA polymerase II elongation. In yeast, this mark in turn recruits epigenetic regulators to reset the chromatin to a relatively repressive ...
Meikar Oliver O Institute of Biomedicine, Department of Physiology, University of Turku, Turku FI-20520, - - 2014
The genome of male germ cells is actively transcribed during spermatogenesis to produce phase-specific protein-coding mRNAs and a considerable amount of different noncoding RNAs. Ribonucleoprotein (RNP) granule-mediated RNA regulation provides a powerful means to secure the quality and correct expression of the requisite transcripts. Haploid spermatids are characterized by a ...
Williams Dustin R DR The Department of Molecular Genetics, The Ohio State University, 105 Biological Sciences Building, 484 West 12th Avenue, Columbus, OH 43210, - - 2014
The segmental structure of the axial skeleton is formed during somitogenesis. During this process, paired somites bud from the presomitic mesoderm (PSM), in a process regulated by a genetic clock called the segmentation clock. The Notch pathway and the Notch modulator Lunatic fringe (Lfng) play multiple roles during segmentation. Lfng ...
Pandurangan Ak A Department of Biochemistry, Cell Biology, University of Madras, Chennai, Tamil Nadu, - - 2014
The present investigation deals with the antimetastatic role of luteolin (LUT) by inhibiting matrix metalloproteinase (MMP)-9 and -2 in azoxymethane (AOM)-induced colon carcinogenesis in Balb/C mice. Animals received AOM at a dosage of 15 mg/kg body weight intraperitoneally once a week for 3 weeks. AOM-induced mice was treated with LUT ...
Cho Sunghee S School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, - - 2014
Spinal muscular atrophy (SMA) is an autosomal recessive genetic disease, which causes death of motor neurons in the anterior horn of spinal cord. Genetic cause of SMA is the deletion or mutation of SMN1 gene, which encodes the SMN protein. Although SMA patients include SMN2 gene, a duplicate of SMN1 ...
Shatsky Ivan N IN Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University , Moscow , Russia - - 2014
Abstract The conventional paradigm of translation initiation in eukaryotes states that the cap-binding protein complex eIF4F (consisting of eIF4E, eIF4G and eIF4A) plays a central role in the recruitment of capped mRNAs to ribosomes. However, a growing body of evidence indicates that this paradigm should be revised. This review summarizes ...
Nohara Kazunari K Division of Endocrinology, Metabolism and Molecular Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA; Comprehensive Center on Obesity, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA; Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, - - 2014
Androgen excess in women is associated with visceral adiposity. However, little is known on the mechanism through which androgen promotes visceral fat accumulation. To address this issue, female mice to chronic androgen excess using 5α-dihydrotestosterone (DHT) and studied the regulation of energy homeostasis was exposed. DHT induced a leptin failure ...
Khan Usman A UA School of Medical Science, Griffith University, Gold Coast, QLD, 4215, Australia; School of Dentistry and Oral Health, Griffith University, Gold Coast, QLD, 4215, - - 2014
Ostoeclasts and foreign body giant cells (FBGCs) are both derived from the fusion of macropahges. These cells are seen in close proximity during foreign body reactions, therefore it was assumed that they might interact with each other. The aim was to identify important genes that are expressed by osteoclasts and ...
Chen H H Genetic Engineering Research Centre, College of Life Science, Chongqing University, Chongqing, China; Institute of Plant Physiology and Ecology, Chinese Academy of Sciences Key Laboratory of Insect Developmental and Evolutionary Biology, CAS, Shanghai, - - 2014
Cysteine proteinases (Cyps) play vital roles in many biological processes, including physiological and pathological reactions. In the present study, we cloned a full cDNA of SlCyp, encoding a 344-amino-acid protein from Spodoptera litura. The putative amino acid sequence shared >75% identity with Cyps from other insects. A phylogenetic analysis revealed ...
Caruso Michael A MA Department of Biological Sciences, North Dakota State University, Fargo, ND 58105 - - 2014
We used rainbow trout as a model to study the regulation of the multiple and distinct insulin (INS) and insulin receptor (IR) mRNAs by somatostatin (SS). Implantation of SS reduced growth of animals without affecting food intake. SS decreased INS1 and INS2 expression in Brockmann bodies, but increased INS1 and ...
Al Rayyan Numan N Department of Human Nutrition, Foods and Exercise, 1981 Kraft Drive, ILSB 1020 (0913), Virginia Tech, Blacksburg, VA 24061; Current Position, School of Medicine, Department of Pharmacology and Toxicology, 505 South Hancock Street, Clinical and Translational Research Building, University of Louisville, Louisville, KY - - 2014
Mice with a deletion of the hypothalamic basic helix-loop-helix transcription factor Nhlh2 display adult onset obesity. We have previously shown that Nhlh2 expression is induced by leptin. In this study, we identify a small proximal leptin-responsive promoter region in the Nhlh2 gene. This 163 bp promoter contains five putative binding ...
Zhao Lina L Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, - - 2014
Forkhead (Fox) transcription factors display functional diversity and are involved in various metabolic and developmental processes. The Spodoptera exigua Fox (SeFox) encodes a protein of 353 amino acids with a theoretical molecular mass of approximately 38.99 kDa and an isoelectric point of 8.86. qPCR results revealed that SeFox was expressed ...
Hua Liushuai L College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, China ; Guangdong Provincial Public Laboratory for Wild Animal Conservation and Management, Guangdong Entomological Institute (South China Institute of Endangered Animals), Guangzhou, - - 2014
As the master regulator of adipogenesis, peroxisome proliferator-activated receptor gamma (PPARG) is required for the accumulation of adipose tissue and hence contributes to obesity. A previous study showed that the substitution of +20A>G in PPARG changed the 7(th) amino acid from Asp to Gly, creating a mutant referred to as ...
Weng Shaozheng S Key Laboratory of Environment and Health, Fujian Medical University, Fuzhou 350004, - - 2014
Cadmium (Cd) impairs ovary structure and function in mature animals. However, the influence of Cd on follicle development from weaning to maturity is obscure. In the current study, 21-day-old Wistar rats were administered Cd chloride at doses of 0, 0.5, 2.0 and 8.0mg/kg body weight once a day for eight ...
Qin Guohua G Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China; The College of Environmental Science and Resources, Shanxi University, Taiyuan, Shanxi 030006, - - 2014
Chlorpyrifos is a typical organophosphate pesticide and is among the most widely used worldwide. The objective of the present investigation was to assess the effect of chlorpyrifos exposure on glutathione S-transferase in Locusta migratoria. In the present study, chlorpyrifos (0.1, 0.2, and 0.4mgg(-1) body weight) was topically applied in the ...
Simpson Clare E - - 2014
The relocalization of translationally repressed mRNAs to mRNA Processing bodies (P-bodies) is a key consequence of cellular stress across many systems. P-bodies harbor mRNA degradation components and are implicated in mRNA decay, but the relative timing and control of mRNA relocalization to P-bodies is poorly understood. We used the MS2-GFP ...
Hutten Saskia - - 2014
Cajal bodies are nuclear structures involved in snRNP and snoRNP biogenesis, telomere maintenance and histone mRNA processing. Recently, the SUMO isopeptidase USPL1 was identified as a Cajal body component essential for cellular growth and Cajal body integrity. However, a cellular function for USPL1 is so far unknown. Here, we use ...
Lu Zhipeng - - 2014
Sm proteins are multimeric RNA-binding factors, found in all three domains of life. Eukaryotic Sm proteins, together with their associated RNAs, form small ribonucleoprotein (RNP) complexes important in multiple aspects of gene regulation. Comprehensive knowledge of the RNA components of Sm RNPs is critical for understanding their functions. We developed ...
Yamanaka Tomoyuki T 1] Department of Neuroscience for Neurodegenerative Disorders, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan [2] Laboratory for Structural Neuropathology, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan [3] Laboratory for Molecular Mechanisms of Thalamus Development, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan [4] CREST (Core Research for Evolutionary Science and Technology), JST, 7, Gobancho, Chiyoda-ku, Tokyo 102-0076, - - 2014
Nuclear transcription factor-Y (NF-Y), a key regulator of cell-cycle progression, often loses its activity during differentiation into nonproliferative cells. In contrast, NF-Y is still active in mature, differentiated neurons, although its neuronal significance remains obscure. Here we show that conditional deletion of the subunit NF-YA in postmitotic mouse neurons induces ...
Li June J Department of Translational Molecular Pathology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas, United States of - - 2014
Promyelocytic leukemia nuclear bodies (PML NBs) are comprised of PML and a striking variety of its associated proteins. Various cellular functions have been attributed to PML NBs, including the regulation of gene expression. We report here that induced expression of PML recruits Sp1 into PML NBs, leading to the reduction ...
Dougherty Jonathan D JD Baylor College of Medicine, United - - 2013
We have previously shown that poliovirus infection disrupts cytoplasmic P-bodies in infected mammalian cells. During the infectious cycle, poliovirus causes the directed cleavage of Dcp1a and Pan3, coincident with dispersion of P-bodies. We now show that expression of Dcp1a prior to infection surprisingly restricts poliovirus infection. This inhibition of infection ...
Visch Valentijn T VT a Faculty of Industrial Design Engineering , Technical University Delft , Delft , The - - 2013
In two studies, the robustness of anger recognition of bodily expressions is tested. In the first study, video recordings of an actor expressing four distinct emotions (anger, despair, fear, and joy) were structurally manipulated as to image impairment and body segmentation. The results show that anger recognition is more robust ...
Nguyen Tu N TN Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA and Research Center for Infectious Diseases, University of Würzburg, 97080 Würzburg, - - 2013
Monoallelic expression within a gene family is found in pathogens exhibiting antigenic variation and in mammalian olfactory neurons. Trypanosoma brucei, a lethal parasite living in the human bloodstream, expresses variant surface glycoprotein (VSG) from 1 of 15 bloodstream expression sites (BESs) by virtue of a multifunctional RNA polymerase I. The ...
Zuo Yu-Han YH Department of Entomology, National Chung Hsing University, Taichung, - - 2013
The sexual difference in gene expression in fat body between 8- and 10-day-old male and female Bactrocera dorsalis was examined using suppression subtractive hybridization. A total of 952 clones were sequenced and searched using BLAST from the subtracted cDNA library. About 22% of these clones showed homology with detoxification enzymes ...
Kamenska Anastasiia A Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB21QW, UK and MRC Toxicology Unit, University of Leicester, Lancaster Road, Leicester LE19HN, - - 2013
A key player in translation initiation is eIF4E, the mRNA 5' cap-binding protein. 4E-Transporter (4E-T) is a recently characterized eIF4E-binding protein, which regulates specific mRNAs in several developmental model systems. Here, we first investigated the role of its enrichment in P-bodies and eIF4E-binding in translational regulation in mammalian cells. Identification ...
Yoshida Ken-Ichi - - 2013
Bradyrhizobium japonicum USDA110, a soybean symbiont, is capable of accumulating a large amount of poly-beta-hydroxybutyrate (PHB) as an intracellular carbon storage polymer during free-living growth. Within the genome of USDA110, there are a number of genes annotated as paralogs of proteins involved in PHB metabolism, including its biosynthesis, degradation, and ...
Füllgrabe Jens J Department of Oncology Pathology, Cancer Centrum Karolinska, Karolinska Institutet, Stockholm 17176, - - 2014
Autophagy is a conserved process by which cytoplasmic components are degraded by the lysosome. It is commonly seen as a cytoplasmic event and, until now, nuclear events were not considered of primary importance for this process. However, recent studies have unveiled a transcriptional and epigenetic network that regulates autophagy. The ...
Hussain Mazhar M Australian Infectious Disease Research Centre; School of Biological Sciences; The University of Queensland; Brisbane, QLD - - 2013
Argonaute proteins (AGOs) are vital components of the RNA-induced silencing complex in gene silencing. AGOs are indispensable for microRNA (miRNA) biogenesis as well as function, and are intracellularly localized to both the cytoplasm and the nucleus. Cytoplasmic AGO-miRNA complexes are mainly involved in cleavage or translational repression of target mRNAs ...
Ishikawa Hideaki H Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan, Metabolome Division, Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Sanbancho 5, Chiyoda-ku, Tokyo 102-0075, Japan, Department of Chemistry, Graduate School of Sciences and Engineering, Tokyo Metropolitan University, 1-1 Minamiosawa, Hachiouji-shi, Tokyo 192-0397, Japan, Department of Bioengineering, United Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan and Biomolecular Characterization Team, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, - - 2013
The U1 small nuclear ribonucleoprotein (snRNP) plays pivotal roles in pre-mRNA splicing and in regulating mRNA length and isoform expression; however, the mechanism of U1 snRNA quality control remains undetermined. Here, we describe a novel surveillance pathway for U1 snRNP biogenesis. Mass spectrometry-based RNA analysis showed that a small population ...
Jin Guoxiang - - 2013
The tumor suppressor promyelocytic leukaemia protein (PML) is predominantly localized in the nucleus, where it is essential for the formation and stabilization of the PML nuclear bodies (PML-NBs). PML-NBs are involved in the regulation of numerous cellular functions, such as tumorigenesis, DNA damage and antiviral responses. Despite its nuclear localization, ...
Biros Erik - - 2013
Abdominal aortic aneurysm (AAA) is a potentially life threatening late onset degenerative condition. MicroRNAs, the small non-coding RNA molecules that regulate gene expression, have been previously shown to be associated with a broad range of human pathologies, including cardiovascular diseases. The aim of this study was to identify AAA-associated microRNAs ...
Retnoaji Bambang - - 2013
During vertebrate development, the primary body axis elongates towards the posterior and is periodically divided into somites, which give rise to the vertebrae, skeletal muscles and dermis. Somites form periodically from anterior to posterior, and the anterior somites form in a more rapid cycle than the posterior somites. However, how ...
Singh M Dhruba - - 2013
Polyglutamine or poly(Q) disorders are dominantly inherited neurodegenerative diseases characterised by progressive loss of neurons in cerebellum, basal ganglia and cortex in adult human brain. Overexpression of human form of mutant SCA3 protein with 78 poly(Q) repeats leads to the formation of inclusion bodies and increases the cellular toxicity in ...
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