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Yabe Tomio T Faculty of Applied Biological Sciences, Department of Applied Life Science, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan, - - 2015
In situ hybridization provides information for understanding the localization of gene expression in various tissues. The relative expression levels of mRNAs in a single cell can be sensitively visualized by this technique. Furthermore, since in situ hybridization is a histological technique, tissue structure is maintained after fixation, and it is ...
Xie Jun-Pei JP Department of Gastroenterology, The Chenggong Hospital Affiliated to Xiamen University, Xiamen, Fujian 361003, P.R. - - 2014
This study aimed to investigate the expression and clinical significance of enoyl coenzyme A hydratase, short chain, 1 (ECHS1), in patients with colorectal cancer (CRC). The ECHS1 protein expression as detected by immunohistochemistry in 148 CRC specimens was evaluated and compared by clinical pathology and prognosis; 38 specimens from proximal ...
Chen Yan-Shan YS Department of Gynecology and Obstetrics, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, P.R. - - 2014
The aim of the present study was to explore the association between the expression of microRNA (miRNA)-181b and plasminogen activator inhibitor-1 (PAI-1) in the placental tissue of pregnant females with a hypertensive disorder complicating pregnancy (HDCP). Placental tissue samples were obtained from 48 patients with HDCP and 40 females with ...
Jia Cundong C Department of Medical Oncology, The Affiliated Tumor Hospital of Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. - - 2014
The aim of the present study was to investigate the expression level of TWIST1 in B-cell non-Hodgkin lymphoma (BNHL) and its association with the clinicopathological characteristics of BNHL. Expression levels of TWIST1 were analyzed in patients with BNHL (n=45) and lymphadenosis (n=21) using immunohistochemical staining and western blot analysis. In ...
Zhang Juan J Department of Obstetrics and Gynecology, Airforce General Hospital, Beijing 100142, P.R. - - 2014
This study aimed to investigate the expression of metastasis suppressor 1 (MTSS1) in cervical intraepithelial neoplasia (CIN) and malignant cervical tissues, and the role of MTSS1 in carcinogenesis. MTSS1 expression was detected by immunohistochemistry in 147 cervical tissue specimens collected from 30 healthy individuals, 30 patients with cervical CIN I, ...
Song Yu Y Department of Oncology, Zhangjiagang First People's Hospital, Suzhou, Jiangsu 215600, P.R. - - 2014
The present study aimed to observe the methylation status of the CpG islands at the human hedgehog interacting protein (HHIP) gene in gastric cancer tissues, peritumoral tissues and the AGS cell line, to analyze the association between the methylation status of the CpG islands and the tumorigenesis of gastric cancer. ...
Kim Ji Young JY Department of Dermatology, Cutaneous Biology Research Institute, Severance Hospital, Yonsei University College of Medicine, Seoul, - - 2014
Recent findings of increased cathelicidin protein and its proteolytic fragments in rosacea suggest a pathogenic role for cathelicidin in this disease. The relationship between cathelicidin and protease-activated receptor 2 (PAR-2) is therefore of interest, as PAR-2, expressed principally in keratinocytes, regulates pro-inflammatory cytokine expression in the skin. The purpose of ...
Grammen Carolien - - 2014
In order to reach sufficiently high tissue concentrations and thus be effective, vaginally applied anti-HIV microbicides that are active at the level of the immune cells must permeate across the cervicovaginal mucosal layer. Cellular efflux transporters, such as Pgp, BCRP and MRP-2, have been demonstrated to greatly affect drug disposition ...
Hakki Sema S SS Selcuk University, Faculty of Dentistry, Department of Periodontology, Konya, - - 2014
Aim; Cell-based therapy employing mesenchymal stem cells (MSCs) seems promising to obtain regeneration of dental tissues. A comparison of tissue sources, including periodontal ligament (PDL) versus pulp (P), could provide critical information to select an appropriate MSC population for designing predictable regenerative therapies. The purpose of this study was to ...
Barrie Elizabeth S ES Center for Pharmacogenomics, The Ohio State University - - 2014
Rationale: Dopamine β-hydroxylase (DBH) catalyzes the conversion of dopamine to norepinephrine in the CNS and peripherally. DBH variants are associated with large changes in circulating DBH and implicated in multiple disorders; yet causal relationships and tissue-specific effects remain unresolved. Objective: To characterize regulatory variants in DBH, effect on mRNA expression, ...
Takahashi Toshiaki T National Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin 12, - - 2014
Normal development of the fetal diaphragm requires muscularization of the diaphragm as well as the structural integrity of its underlying connective tissue components. Developmental mutations that inhibit the formation of extracellular matrix (ECM) have been shown to result in congenital diaphragmatic hernia (CDH). Copper (Cu) is an important element during ...
Koizumi Hiroki H Department of Otorhinolaryngology-Head and Neck Surgery, University of Occupational and Environmental Health, - - 2014
We investigated the electrical impedance of and the expressions of tight junction molecules in the cholesteatoma epithelium to provide supporting evidence for the acid lysis theory of bone resorption in middle ear cholesteatoma. Study subjects were patients with primary acquired middle ear cholesteatoma and those with non-cholesteatomatous chronic otitis media ...
Yogarajah Thaneswary T Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Penang, - - 2014
This study was conducted to determine the mRNA and protein expression levels of peroxisome proliferator‑activated receptors (PPARs) in visceral adipose tissue, as well as serum adipokine levels, in Sprague Dawley rats. The rats were fed either a normal (control rats) or excessive (experimental rats) intake of food for 8 or 16 weeks, ...
Sui Linlin - - 2014
As the interface between the mother and the developing fetus, the placenta is believed to play an important role in assisted reproductive technology (ART)-induced aberrant intrauterine and postnatal development. However, the mechanisms underlying aberrant placentation remain unclear, especially during the extra-embryonic tissue development and early stage of placental formation. Using ...
Letizia Claudio C Specialized Center of Secondary - - 2014
Context: Interplay between adipose tissue and adrenal glands has been recently suggested, without well-founded actions of locally adipose tissue surrounding the adrenal glands. Objective: We hypothesized that the local expression of leptin and adiponectin can be associated with pathological changes of the adrenal glands. Patients and main outcome measures: we ...
Yang Chao-Chun CC Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; International Research Center of Wound Repair and Regeneration, National Cheng Kung University, Tainan, - - 2014
Adipose tissue encircles the lower portion of anagen hair follicles and may regulate hair cycle progression. Since leptin is a major adipokine, its level of expression from the dermal white adipose tissue during hair cycle progression was studied. The result shows that leptin level is differentially expressed during hair cycle, ...
Chen Yu Y Department of Medicine and Research Center Montreal Heart Institute and Université de Montréal; Montreal, Quebec, Canada; Department of Pharmacology and Therapeutics McGill University, Montreal, Quebec, - - 2014
Heart failure (HF) causes left-atrial (LA) and left-ventricular (LV) remodeling, with particularly-prominent changes in LA that create a substrate for atrial fibrillation (AF). MicroRNAs (miRs) are potential regulators in cardiac remodeling. This study evaluated time-dependent miR expression-changes in LA and LV tissue, fibroblasts and cardiomyocytes in experimental HF. HF was ...
Kern Philip A PA Department of Medicine (P.A.K., B.S.F., B.Z.), Division of Endocrinology, and the Barnstable Brown Diabetes and Obesity Center, University of Kentucky, Lexington, Kentucky 40536; Division of Endocrinology (N.R.), University of Colorado Denver, Denver, Colorado 80220; Eastern Colorado Veterans Health Care System (N.R.), Denver, Colorado 80220; Department of Pediatrics (R.E.M.), University of Arkansas for Medical Sciences, Little Rock, Arkansas; College of Public Health (P.M.W.) and College of Health Sciences (E.E.D.-V.), University of Kentucky, Lexington, Kentucky - - 2014
Context: Although brown adipose tissue (BAT) activity is increased by a cold environment, little is known of the response of human white adipose tissue (WAT) to the cold. Design: We examined both abdominal and thigh sc WAT from 71 subjects who were biopsied in the summer or winter, and adipose ...
Ahani Narges N Faculty of Science, University of Zabol, Zabol, Iran, - - 2014
Glioblastoma multiforme (GBM) is considered to be one of the most invasive human cancers, characterized by a high mortality rate and an average survival is <1 year. These tumors are highly aggressive and insensitive to conventional radio and chemotherapy. An interesting aspect of glioblastoma is the association of active human cytomegalovirus ...
Yang Zhiying Z Nephrology Research Institute of Shandong University, The Second Hospital of Shandong University, Shandong University , Jinan, Shandong , - - 2014
Abstract Background: Overwhelming evidences suggest epithelial to mesenchymal transition (EMT) of tubular epithelial cells contributes to renal fibrosis of chronic kidney disease (CKD). Connective tissue growth factor (CTGF) plays an important role in the pathogenesis of EMT. However, the molecular mechanisms that regulate cell behaviors are not clear. Objective: The ...
Akour Amal A AA Department of Pharmacotherapy and Outcomes Science, School of Pharmacy, Virginia Commonwealth University, Richmond, - - 2014
The aims of this study were to characterize megalin expression in human term and preterm placental villous tissues and to assess the impact of gestational age and sample storage on receptor expression. Placental tissue samples were collected from pregnant women undergoing term and preterm Caesarean deliveries. Placental villous tissues were ...
O Sadashiv S Department of Physiology, King George's Medical University, Lucknow 226003, - - 2014
Adipose tissue secretes various kinds of adipokines that controls the glucose and lipid metabolism in humans. The abdominal visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) both are associated with metabolic syndrome and insulin resistance. IL-6 is one of the adipokines, which promotes insulin resistance and dyslipidemia in humans. ...
Sullivan K J KJ Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6, - - 2014
Freeze tolerance is a critical winter survival strategy for the wood frog, Rana sylvatica. In response to freezing, a number of genes are upregulated to facilitate the survival response. This includes fr10, a novel freeze-responsive gene first identified in R. sylvatica. This study analyzes the transcriptional expression of fr10 in ...
Land Stephen C SC Division of Cardiovascular and Diabetes Medicine, Medical Research Institute, Ninewells Hospital and Medical School, University of Dundee, Dundee, DD1 9SY, Scotland, UK. Electronic address: - - 2014
The existence of a nutrient sensitive "autocatakinetic" regulator of embryonic tissue growth has been hypothesised since the early 20(th) century, beginning with pioneering work on the determinants of fetal size by the Australian physiologist, Thorburn Brailsford-Robertson. We now know that the mammalian target of rapamycin complexes (mTORC1 and 2) perform ...
Murahovschi Veronica V Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany; Department of Endocrinology, Diabetes and Nutrition, Campus Benjamin Franklin, Charité - Universitätsmedizin, Berlin, - - 2014
WISP1 (Wnt1 inducible signaling pathway protein 1, CCN4) is a member of the secreted extracellular matrix-associated proteins of the CCN family and target gene of the Wingless-type (WNT) signaling pathway. Growing evidence links the WNT signaling pathway to the regulation of adipogenesis and low-grade inflammation in obesity. Here we aim ...
Yien Yvette Y YY Department of Developmental & Regenerative Biology, Mount Sinai School of Medicine, New York, NY, - - 2014
Alternative splicing has emerged as a vital way to expand the functional repertoire of a set number of mammalian genes. For example, such changes can dramatically alter the function and cellular localization of transcription factors. With this in mind, we addressed whether Eklf/Klf1 mRNA, coding for a transcription factor that ...
Tamhane Tripti - - 2014
Abstract Cysteine cathepsins are expressed in most tissues, including the gastrointestinal tract. We demonstrated an involvement of mouse intestinal cathepsin B in extracellular matrix remodeling for regeneration from trauma. The present study aimed at elucidating roles of cysteine cathepsins in the non-traumatized gastrointestinal tract of mice. Thus we investigated expression ...
Rozier Frédérique F Laboratoire Reproduction et Développement des Plantes, Ecole Normale Supérieure Lyon, Lyon, - - 2014
In situ mRNA hybridization is one of the most powerful techniques for analyzing patterns of gene expression. However, its usefulness is limited in complex plant tissues by the need to fix, embed and section samples before localizing the desired mRNA. Here we present a robust whole-mount in situ hybridization method ...
Wu Xiu-Juan XJ Department of Dermatology and Venereology, Anhui Medical University, Hefei, Anhui, - - 2014
Kaposi's sarcoma (KS) is a multicentric angioproliferative tumor of mesenchymal origin. The molecular and biologic aspects of KS are not fully understood. MicroRNAs are non-protein-coding small RNAs in the size range 19-25 nucleotides (nt) that play important roles in biological processes, including cellular differentiation, proliferation, and death. We performed a ...
Wordinger Robert J RJ *North Texas Eye Research Institute †Department of Cell Biology and Immunology, University of North Texas Health Science Center at Fort Worth, Fort Worth, - - 2014
The mechanical properties of the extracellular matrix (ECM) play an important role in maintaining cellular function and overall tissue homeostasis. Emerging evidence suggests that biomechanical modifications of the ECM may be initiators and/or drivers of disease, exemplified by increased tissue stiffness. Specific ECM cross-linking enzymes (tissue transglutaminase, lysyl oxidase, and ...
Hayashi Shinichi S Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aramaki-Aza-Aoba 6-3, Aoba-ku, Sendai 980-8578, - - 2014
The size and shape of tissues are tightly controlled by synchronized processes among cells and tissues to produce an integrated organ. The Hippo signaling pathway controls both cell proliferation and apoptosis by dual signal-transduction states regulated through a repressive kinase cascade. Yap1 and Tead, transcriptional regulators that act downstream of ...
Zhu Jiantang - - 2014
BackgroundThe endoplasmic reticulum chaperone binding protein (BiP) is an important functional protein, which is involved in protein synthesis, folding assembly, and secretion. In order to study the role of BiP in the process of wheat seed development, we cloned three BiP homologous cDNA sequences in bread wheat (Triticum aestivum), completed ...
Zhang Peng P Neurosurgery Department, Yi He Hospital of Zhengzhou, 69 Nongye Street, Zhengzhou, Henan, China, - - 2014
Rho-associated protein kinase 1 (ROCK1), a serine/threonine protein kinase, affects cell invasion and migration by changing the status of the cytoskeleton. In recent years, ROCK1 was found to be overexpressed in a variety of tumors. However, the information of ROCK1 in glioma still remains elusive. In our study, the expression ...
Li Hong-Dong HD Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, Michigan, - - 2014
Canonical isoforms in different databases have been defined as the most prevalent, most conserved, most expressed, longest, or the one with the clearest description of domains or post-translational modifications. In this article, we revisit these definitions of canonical isoforms based on functional genomics and proteomics evidence, focusing on mouse data. ...
Yu Fa-Xing FX Department of Pharmacology and Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093 and Children's Hospital and Institute of Biomedical Sciences, Fudan University, Shanghai, China - - 2014
The Hippo pathway plays a crucial role in regulating tissue homeostasis and organ size, and its deregulation is frequently observed in human cancer. Yap is the major effector of and is inhibited by the Hippo pathway. In mouse model studies, inducible Yap expression in multiple tissues results in organ overgrowth. ...
Choi Ye Na YN Department of Biology, Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, Republic of - - 2014
The fragile X mental retardation 1 (FMR1) protein binds mRNA and acts as a negative regulator of translation. Lack of FMR1 causes the most common neurological disorder, fragile X syndrome, while its overexpression is associated with metastasis of breast cancer. Its activity has been well-studied in nervous tissue, but recent ...
Kicheva Anna A Medical Research Council (MRC), National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW71AA, - - 2014
Development requires tissue growth as well as cell diversification. To address how these processes are coordinated, we analyzed the development of molecularly distinct domains of neural progenitors in the mouse and chick neural tube. We show that during development, these domains undergo changes in size that do not scale with ...
Taha Masoumeh Fakhr MF Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB) , Tehran, - - 2014
Natriuretic peptide precursor-A (NPPA) is an early and specific marker for functional myocardium of the embryonic heart. NPPA gene encodes for a precursor of atrial natriuretic peptide (ANP). So far, three alternatively spliced variants have been reported for NPPA in human. In mouse, no alternatively spliced transcript of NPPA has ...
Gruchy Jennette R JR Department of Pathology, Capital District Health Authority and Dalhousie University, Halifax, NS, - - 2014
Immunohistochemistry is used on cell blocks constructed from cytopathology samples fixed in methanol-based fixatives, such as CytoLyt (Cytyc Corp), and on surgical pathology tissues exposed to decalcifying agents, often without technical validation. We evaluated a panel of commonly utilized antibodies in normal tissues exposed to differing preanalytic conditions as follows: ...
Alder Olivia O Terry Fox Laboratory, BC Cancer Agency, Vancouver, BC V5Z1L3, - - 2014
Cell fate acquisition is heavily influenced by direct interactions between master regulators and tissue-specific enhancers. However, it remains unclear how lineage-specifying transcription factors, which are often expressed in both progenitor and mature cell populations, influence cell differentiation. Using in vivo mouse liver development as a model, we identified thousands of enhancers ...
Frauenfelder Claire C Department of Surgery, Flinders Medical Centre and Flinders University, Adelaide, South Australia, - - 2014
Thickened secretions, mucosal edema, and polyp formation are pathological features in chronic rhinosinusitis (CRS) that could theoretically be caused by aberrant water flow through sinonasal mucosa. Aquaporins (AQPs) are a family of proteins with roles in water transport, with tissue-specific expression profiles. This study aims to determine if AQP expression ...
Wu Hsiao-Li HL Department of - - 2014
Context: In adipose tissue (AT) microRNA-93 (miR-93) is significantly overexpressed in PCOS women and non-PCOS women with insulin resistance (IR). Overexpressed miR-93 directly inhibits GLUT4, impairing both glucose metabolism and insulin sensitivity. The mechanisms behind increased miR-93 expression are unclear. Objective: Our objective was to determine whether miR-93 expression is ...
Liu Qiang Q Department of Neurosurgery, The Third Xiangya Hospital, Central South University, 138 Tongzipo Road, Hexi Yuelu District, Changsha, Hunan, 410013, - - 2014
miR-132 was found to be overexpressed in glioma; however, its clinical significance has not been investigated. In the present study, we evaluated the association between miR-132 and clinicopathological parameters and prognosis. Quantitative real-time PCR was used to analyze the expression of miR-132 in 113 cases of glioma and 36 cases ...
Higa Romina R Laboratory of Reproduction and Metabolism, CEFYBO-CONICET, School of Medicine, University of Buenos Aires, Paraguay 2155, 17th floor (1121ABG) Buenos Aires, Argentina. Electronic address: - - 2014
Maternal diabetes induces a pro-oxidant/pro-inflammatory intrauterine environment related to the induction of congenital anomalies. Peroxisome proliferator activated receptors (PPARs) are transcription factors that regulate antioxidant and anti-inflammatory pathways. We investigated whether maternal diets supplemented with olive oil, enriched in oleic acid, a PPAR agonist, can regulate the expression of PPAR ...
Moindjie Hadia - - 2014
PreImplantation Factor (PIF) is a peptide secreted by viable mammalian embryos. Moreover, it can be detected in pregnant women circulation. Recently, it was shown that PIF promotes invasion in trophoblast cell lines in vitro. Successful human embryo implantation depends on a deep and highly controlled invasion of extravillous trophoblast (EVT) ...
Pamnani Mamta M Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad 211004, - - 2014
Sox11, a member of the group C Sox transcription factor family, is predominantly expressed in the neurogenic areas of the adult brain. In the embryo, Sox11 is expressed in the developing nervous system in both glial and neuronal lineages. Its expression exhibits a dynamic pattern with respect to both expression ...
Visconti B B Unit of Dermatology, Department of Internal Medicine and Medical Specialties, 'Sapienza' University of Rome, Rome, - - 2014
Cell junctions are crucial for the formation and maintenance of the paracellular barrier and for cell polarity in simple epithelia and endothelia. Altered localization and formation of tissue junction proteins in the epidermis have been described in plaque-type psoriasis. Vitamin D receptor (VDR) is a nuclear hormone involved in anti-proliferative ...
Al-Azri Abdul R AR Faculty of Health Sciences, School of Dentistry, The University of Adelaide, Adelaide, SA, Australia; Ministry of Health, Muscat, Sultanate of - - 2014
Matrix metalloproteinases (MMPs) are involved in both maintenance of healthy mucosa and mediation of several pathologies. Recently, MMPs and their inhibitors have attracted attention as potential mediators of mucositis. We investigated tissue expression of MMP-3 and MMP-9 over time in a pre-clinical model of irinotecan-induced oral mucositis (OM). Eighty-one female ...
Shiratori Hidetaka H Developmental Genetics Group, Graduate School for Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan. Electronic address: - - 2014
The transcription factor Pitx2c is expressed in primordial visceral organs in a left-right (L-R) asymmetric manner and executes situs-specific morphogenesis. Here we show that Pitx2c is also L-R asymmetrically expressed in the developing mouse limb. Human PITX2c exhibits the same transcriptional activity in the mouse limb. The asymmetric expression of ...
Freise Christian C Department of Gastroenterology, Infectiology and Rheumatology, Charité - University Medicine Berlin, Campus Benjamin Franklin, Berlin, - - 2014
In liver fibrosis, activated hepatic stellate cells (HSC) secrete excess extracellular matrix, thus, represent key targets for antifibrotic treatment strategies. Intermediate-conductance Ca(2+) -activated K(+) -channels (KCa3.1) are expressed in non-excitable tissues affecting proliferation, migration and vascular resistance rendering KCa3.1 potential targets in liver fibrosis. So far, no information about KCa3.1 ...
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