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Tamhane Tripti - - 2014
Abstract Cysteine cathepsins are expressed in most tissues, including the gastrointestinal tract. We demonstrated an involvement of mouse intestinal cathepsin B in extracellular matrix remodeling for regeneration from trauma. The present study aimed at elucidating roles of cysteine cathepsins in the non-traumatized gastrointestinal tract of mice. Thus we investigated expression ...
Visconti B B Unit of Dermatology, Department of Internal Medicine and Medical Specialties, 'Sapienza' University of Rome, Rome, - - 2014
Cell junctions are crucial for the formation and maintenance of the paracellular barrier and for cell polarity in simple epithelia and endothelia. Altered localization and formation of tissue junction proteins in the epidermis have been described in plaque-type psoriasis. Vitamin D receptor (VDR) is a nuclear hormone involved in anti-proliferative ...
Al-Azri Abdul R AR Faculty of Health Sciences, School of Dentistry, The University of Adelaide, Adelaide, SA, Australia; Ministry of Health, Muscat, Sultanate of - - 2014
Matrix metalloproteinases (MMPs) are involved in both maintenance of healthy mucosa and mediation of several pathologies. Recently, MMPs and their inhibitors have attracted attention as potential mediators of mucositis. We investigated tissue expression of MMP-3 and MMP-9 over time in a pre-clinical model of irinotecan-induced oral mucositis (OM). Eighty-one female ...
Shiratori Hidetaka H Developmental Genetics Group, Graduate School for Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan. Electronic address: - - 2014
The transcription factor Pitx2c is expressed in primordial visceral organs in a left-right (L-R) asymmetric manner and executes situs-specific morphogenesis. Here we show that Pitx2c is also L-R asymmetrically expressed in the developing mouse limb. Human PITX2c exhibits the same transcriptional activity in the mouse limb. The asymmetric expression of ...
Freise Christian C Department of Gastroenterology, Infectiology and Rheumatology, Charité - University Medicine Berlin, Campus Benjamin Franklin, Berlin, - - 2014
In liver fibrosis, activated hepatic stellate cells (HSC) secrete excess extracellular matrix, thus, represent key targets for antifibrotic treatment strategies. Intermediate-conductance Ca(2+) -activated K(+) -channels (KCa3.1) are expressed in non-excitable tissues affecting proliferation, migration and vascular resistance rendering KCa3.1 potential targets in liver fibrosis. So far, no information about KCa3.1 ...
Sasarman Florin F Montreal Neurological Institute and Department of Human Genetics, McGill University, Montreal, QC, H3A 2B4, - - 2014
French Canadian Leigh Syndrome (LSFC) is an early onset, progressive neurodegenerative disorder with a distinct pattern of tissue involvement. Most cases are caused by a founder missense mutation in LRPPRC. LRPPRC forms a ribonucleoprotein complex with SLIRP, another RNA-binding protein, and this stabilizes polyadenylated mitochondrial mRNAs. LSFC fibroblasts have reduced ...
Marangoni Rossana Anderson RA Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo SP, Brazil. Electronic address: - - 2014
Prolylcarboxypeptidase (PRCP), an endothelial cell membrane serine peptidase that inactivates angiotensin II and activates pre-kallikrein, is thought to have anti-hypertensive and anti-proliferative roles in cardiovascular homeostasis. We hypothesized that PRCP function may be altered in heart tissue under conditions that predispose to left ventricle hypertrophy (LVH) in rats. We therefore ...
Krishnaswami Suguna R SR Division of Dermatology, Department of Medicine, Institute for Genomic Medicine, Stem Cell Program, University of California, San Diego, CA, - - 2014
Determining the developmental consequences of activated RAS and its downstream effectors is critical to understanding several congenital conditions caused by either germline or somatic mutations of the RAS pathway. Here we demonstrate that embryonic activation of BRAF in mouse ectoderm triggers both craniofacial and skin defects, including hyperproliferation, loss of ...
Beck Martina M The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK robatzek@TSL.ac.uk - - 2014
Pathogens can colonize all plant organs and tissues. To prevent this, each cell must be capable of autonomously triggering defence. Therefore, it is generally assumed that primary sensors of the immune system are constitutively present. One major primary sensor against bacterial infection is the FLAGELLIN SENSING 2 (FLS2) pattern recognition ...
Qin Bi B Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, Hainan, - - 2014
MYB family proteins regulate a variety of cellular processes in plants. Tapping panel dryness (TPD) in rubber tree (Hevea brasiliensis Muell. Arg.) affects latex biosynthesis and causes serious losses to rubber producers. In this study, a novel SANT/MYB transcription factor gene down-regulated in TPD rubber tree, named as HbSM1, was ...
Yu Yang Y Comprehensive Laboratory, Third Affiliated Hospital, Soochow University, Jiangsu, 213003, Changzhou, People's Republic of China, - - 2014
The present study examined mRNA levels of apolipoprotein M (apoM) and apolipoprotein AI (apoAI) in the term placental tissues obtained from 37 women with normal birth weight neonates and from 37 women with macrosomic neonates (birth body weight ≥4,000 g), and further discussed possible clinical significance of these observations. The mRNA ...
Zhang Xiufeng X Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078, USA. Electronic address: - - 2014
Significance of microRNA-mediated posttranscriptional regulation has been appreciated ever since its discovery. In the tobacco hornworm Manduca sexta, 164 conserved and 16 novel microRNAs have been identified experimentally (Zhang et al., 2012, 2014). To extend the list of microRNAs in this lepidopteran model species and further explore their possible regulatory ...
Shumskaya Valeriya Sergeevna VS Centre "Bioengineering" of Russian Academy of Sciences, Moscow, Russia, - - 2014
The Kaiso protein was originally described as a BTB/POZ zinc-finger transcription factor and a p120-catenin-binding partner. It is a DNA methylation-dependent transcriptional repressor, but its biological role in mice is still unknown. Here, we characterized a Kaiso-specific antibody by examining Kaiso protein distribution by immunofluorescence microscopy in the following tissues ...
Uno Yasuhiro Y Laboratories of Translational Research, Graduate School of Pharmaceutical Sciences, Hokkaido - - 2014
Cytochromes P450 (P450) are important for not only drug metabolism and toxicity, but also biosynthesis and metabolism of cholesterol and bile acids, and steroid synthesis. In cynomolgus macaques, widely used in biomedical research, we have characterized P450 cDNAs, which were isolated as expressed sequence tags of cynomolgus macaque liver. In ...
Kong Xiaocen X Department of Geratology, The First Hospital Affiliated to Nanjing Medical University, Nanjing, People's Republic of - - 2014
Long-term glucocorticoid treatment induces central fat accumulation and metabolic dysfunction. We demonstrate that microRNA-27b plays a central role in the pathogenesis of glucocorticoid-induced central fat accumulation. Overexpression of miR-27b had the same effects as dexamethasone treatment in the inhibition of brown adipose differentiation and the energy expenditure of primary adipocyte. ...
Contreras G Andres GA Michigan State - - 2014
Brown adipocytes (BA) generate heat in response to sympathetic activation and are the main site of non-shivering thermogenesis in mammals. Although most BA are located in classic brown adipose tissue depots, BA are also abundant in the inguinal white adipose tissue (iWAT) prior to weaning. The number of BA is ...
Perez-Rico Consuelo C Ophthalmology, University Hospital Príncipe de Asturias. University of Alcalá, Carretera Alcalá-Meco s/n, Alcalá de Henares, Madrid, 28805, Spain - - 2014
ABSTRACT PURPOSE. Evaluate the expression of several extracellular matrix constituents implicated in the synthesis and reticulation of elastin in human pterygium, according to age and gender of the patients. METHODS. Pterygia and normal conjunctiva samples were divided into groups according to age (<50/≥50 years) and gender. Tissue was subjected to ...
Wu Han H Lawrence Berkeley National Laboratory, Berkeley, California, United States of - - 2014
Short non-coding transcripts can be transcribed from distant-acting transcriptional enhancer loci, but the prevalence of such enhancer RNAs (eRNAs) within the transcriptome, and the association of eRNA expression with tissue-specific enhancer activity in vivo remain poorly understood. Here, we investigated the expression dynamics of tissue-specific non-coding RNAs in embryonic mouse ...
Wei Li-Na LN Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-Sen University, 17th Shou-gou-ling Rd, Guangzhou, 510655, - - 2014
Growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) play crucial roles in follicular development and oocyte maturation. This study aimed to investigate and compare the expression of these proteins in ovarian tissues of women with and without polycystic ovary syndrome (PCOS). Ovarian tissues from 28 patients with ...
Upadhyay Swati - - 2014
Saponins are mainly amphipathic glycosides that posses many biological activities and confer potential health benefits to humans. Inspite of its medicinal attributes most of the triterpenes and enzymes involved in the saponin biosynthesis remains uncharacterized at the molecular level. Since the major steroidal components are present in the roots of ...
Lu Shao-Hua SH The Institute of Skull Base Surgery and Neurooncology at Hunan, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. - - 2014
A number of microRNAs have been identified to be important regulators of tumorigenesis. Previous research has shown that miR-124 is abundantly expressed in normal brain tissue; however, only a few reports have focused on the biological impact of miR-124 on glioma cells, and the underlying mechanisms need to be elucidated. ...
Huang Dong D Department of Trauma and Microsurgery, Non affiliated Guangdong NO.2 People's Hospital of Southern Medical University, Guangzhou, - - 2014
Abstract Purpose: Activation of transforming growth factor-β signaling and matrix metalloproteinases are involved in hypertrophic scar (HS) formation. Compression therapy is known to be an effective approach for the treatment of hypertrophic scarring; however, the underlying molecular mechanisms remain poorly understood. We investigated the relationship between TGF-β signaling activation and ...
Li Zhiqian Z Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China; University of Chinese Academy of Sciences, Beijing 100049, - - 2014
Insect development and metamorphosis are regulated by two major hormones, juvenile hormone and ecdysteroids. Despite being the key regulator of insect developmental transitions, the metabolic pathway of the primary steroid hormone, 20-hydroxyecdysone (20E), especially its inactivation pathway, is still not completely elucidated. A cytochrome P450 enzyme, CYP18A1, has been shown ...
Johnson Zariel I ZI Department of Orthopaedic Surgery and Graduate Program in Cell, Thomas Jefferson University, Philadelphia, PA; Developmental Biology, Thomas Jefferson University, Philadelphia, - - 2014
Degeneration of the intervertebral disc is characterized by changes in proteoglycan status, loss of bound water molecules, decreased tissue osmotic pressure and a resulting mechanical failure of the disc. A similar spectrum of changes is evident in osteoarthritic articular cartilage. When healthy, resident cells in these skeletal tissues respond to ...
Cho Jong-Ho JH College of Pharmacy, Dongguk University, Goyang, Gyeonggi-do 410-773, Republic of - - 2014
Programmed cell death 4 (PDCD4) is a bona fide tumor suppressor protein and plays a critical role in controlling the rate of protein synthesis. Here, we show that TPA selectively activated the S6K1 and ERK1/2 kinases, contributing to PDCD4 proteolysis and Pdcd4 mRNA degradation in HepG2 cells, respectively. In addition, ...
Papa V V Department of Movement, Human and Health Sciences, Section of Health Sciences, University of Rome "Foro Italico", Piazza L. de Bosis 15, 00135, Rome, - - 2014
Cadmium (Cd) is a heavy metal widely distributed throughout the environment as a result of contamination from a variety of sources. It exerts toxic effects in many tissues but scarce data are present as yet on potential effects on skeletal muscle tissue. To evaluate the potential alteration induced by Cd ...
Chen Bin - - 2014
BackgroundThe expression of heparanase (HPSE) was associated with postoperative metastatic recurrence in patients with hepatocellular carcinoma (HCC). The six E-box binding sites in the core promoter of the HPSE gene suggested that transcription factors of E-box such as upstream stimulatory factor (USF) might regulate the transcription of the HPSE gene. ...
Kedia George T GT Hannover Medical School, Division of Surgery, Department of Urology & Urological Oncology, Hannover, Germany. Electronic address: - - 2014
Experimental and clinical studies have suggested a role for phosphodiesterase (PDE) isoenzymes in the control of the human lower urinary tract. This study aimed to investigate the expression of PDE isoenzymes and the effects of PDE inhibitors (PDE-Is) in isolated human urethral smooth muscle (USM). The expression of messenger ribonucleic ...
Tew Simon R SR Department Of Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Neston, Cheshire, CH64 7TE, - - 2014
Objective: mRNA decay rates control not only gene expression levels but also responsiveness to altered transcriptional input. This study examined transcriptome-wide post-transcriptional regulation in both normal and osteoarthritic human articular chondrocytes. Methods: Human articular chondrocytes were isolated from normal or osteoarthritic tissue. Equine articular chondrocytes were isolated from young or ...
Jurberg Arnon Dias AD Instituto Gulbenkian de Ciência. Rua da Quinta Grande 6. 2780-156 Oeiras. - - 2014
Extension of the vertebrate body results from the concerted activity of many signals in the posterior embryonic end. Among them, Wnt3a has been shown to play relevant roles in the regulation of axial progenitor activity, mesoderm formation and somitogenesis. However, its impact on axial growth remains to be fully understood. ...
Zhu Wu W Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. - - 2014
Psoriasis is a multifactorial disease and the mechanisms involved in its pathogenesis remain to be elucidated. FOS‑like antigen 1 (Fra‑1) is a proto‑oncogene. It is a negative inhibitor of activator protein‑1 activity and possesses transforming activity. The effect of and possible mechanisms underlying Fra‑1 in psoriasis remain to be elucidated. ...
Lin Fangjun F Department of aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, - - 2014
Apelin is a recently discovered peptide produced by several tissues with diverse physiological actions mediated by its receptor APJ. In order to better understand the role of apelin in the regulation of appetite in fish, we cloned the cDNAs encoding apelin and APJ, and investigated their mRNA distributions in Ya-fish ...
Zhou Ying-Qun YQ Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. - - 2014
The aim of the present study was to investigate the correlations and possible synergy among the urokinase‑type plasminogen activator receptor (uPAR) isomer D1D2 and integrin α5β1 expression levels, malignant transformation in hepatic cells and the occurrence of liver cancer. The expression site and concentration of uPAR (D1D2) were analyzed using polymerase ...
Xianjun Peng - - 2014
BackgroundThe paper mulberry (Broussonetia papyifera) is one of the multifunctional tree species in agroforestry system and is also commonly utilized in traditional medicine in China and other Asian countries. To identify the transcription factors (TFs) and comprehensively understand their regulatory roles in the growth of the paper mulberry, a global ...
Messenger Scott W SW University of Wisconsin, United - - 2014
Acinar cell zymogen granules (ZG) express 2 isoforms of the vesicle associated membrane protein family (VAMP2 and 8) thought to regulate exocytosis. Expression of tetanus-toxin to cleave VAMP2 in VAMP8 knockout (-/-) acini confirmed that VAMP2 and 8 are the primary VAMPs for regulated exocytosis each contributing ~50% of the ...
Akpinar Ilke I Department of Pediatric Surgery, Akdeniz University Faculty of Medicine, Antalya, Turkey, - - 2014
The pathogenesis of pulmonary hypoplasia associated with congenital diaphragmatic hernia (CDH) remains unclear. Interactions between the epithelium and surrounding mesenchyme play an important role in normal lung morphogenesis. Epimorphin, a stromal protein, plays a role in epithelial morphogenesis and lung branching, both of which are involved in pulmonary hypoplasia. In ...
Aizer Adva - - 2014
The 5'→3' mRNA degradation machinery localizes to cytoplasmic P bodies (PBs), which are non-membranous structures found in all eukaryotes. While PB function has been intensively studied in yeast, less is known in mammalian cells, such as whether PB enzymes are actively engaged in mRNA degradation or if PBs serve as ...
Sauer Eva E Institute of Legal Medicine, University of Bonn, Stiftsplatz 12, 53111 Bonn, - - 2014
Messenger-RNA (mRNA)-based analysis of organ tissues and their differentiation in complex crime stains has recently been introduced as a potential and powerful tool to forensic genetics. Given the notoriously low quality of many forensic samples it seems advisable, though, to substitute mRNA with micro-RNA (miRNA) which is much less susceptible ...
Hou Shengping S 1] The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. [2] Chongqing Key Laboratory of Ophthalmology, Chongqing, China. [3] Chongqing Eye Institute, Chongqing, - - 2014
To identify new genetic risk factors for Vogt-Koyanagi-Harada (VKH) syndrome, we conducted a genome-wide association study of 2,208,258 SNPs in 774 cases and 2,009 controls with follow-up in a collection of 415 cases and 2,006 controls and a further collection of 349 cases and 1,588 controls from a Han Chinese ...
Herdegen Samantha S Neuroscience Program, Dominican University, River Forest, - - 2014
We used a custom-designed microarray and quantitative PCR to characterize the rapid transcriptional response to long-term sensitization training in the marine mollusk Aplysia californica. Aplysia were exposed to repeated noxious shocks to one side of the body, a procedure known to induce a long-lasting, transcription-dependent increase in reflex responsiveness that ...
Liu Jie J College of Life Sciences, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China, - - 2014
The PstICL1 gene, which encodes isocitrate lyase, a key enzyme in the glyoxylate cycle, was cloned and characterized in the biotrophic wheat pathogen Puccinia striiformis f. sp. tritici (Pst). Expression analyses of PstICL1 exhibited high levels of transcripts in ungerminated urediniospores, which showed low isocitrate lyase enzyme activity. In planta, ...
Morotti Alessandro A Dept. of Clinical and Biological Sciences, University of Turin, San Luigi Hospital, Orbassano, - - 2014
Introduction: PTEN plays an essential role in the pathogenesis of Chronic Myeloid Leukemia. Recently, we have shown that BCR-ABL promotes PTEN nuclear exclusion, through the modulation of HAUSP activity. Objectives: here we investigate HAUSP cellular compartmentalization in primary CML samples. Results: while in normal CD34 positive cells HAUSP is expressed ...
Yada Takashi T 1 Nikko Station, National Research Institute of Aquaculture, Tochigi 321-1661, - - 2014
Changes in mRNA levels of prolactin (PRL) during the upstream migration were examined in fry of the amphidromous fish, ayu Plecoglossus altivelis. Quantification of mRNA has been done with real-time PCR and expressed as whole body or pituitary contents depending the body size of fry. PRL mRNA levels of ayu ...
Chen Yan Y Department of Ophthalmology, Sixth People's Hospital Affiliated of Shanghai Jiaotong University, China; Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, - - 2014
Our previous study identified five new heat shock factor 4 (HSF4) mutations in 150 age-related cataract (ARC) patients which indicated that HSF4 mutations may be associated with this disease. Hypoxia-inducible factor (Hif1α) is an important downstream target of HSF4b. It has been found that Hif1α play also important roles in ...
Rajgor Dipen - - 2014
In eukaryotic cells, non-translating mRNAs can accumulate into cytoplasmic mRNP (messenger ribonucleoprotein) granules such as P-bodies (processing bodies) and SGs (stress granules). P-bodies contain the mRNA decay and translational repression machineries and are ubiquitously expressed in mammalian cells and lower eukaryote species including Saccharomyces cerevisiae, Drosophila melanogaster and Caenorhabditis elegans. ...
Castilla-Llorente Virginia - - 2014
RNA granules have been observed in different organisms, cell types and under different conditions, and their formation is crucial for the mRNA life cycle. However, very little is known about the molecular mechanisms governing their assembly and disassembly. The aggregation-prone LSCRs (low-sequence-complexity regions), and in particular, the polyQ/N-rich regions, have been ...
Pizzinga Mariavittoria M *Faculty of Life Sciences, Michael Smith Building, The University of Manchester, Oxford Road, Manchester M13 9PT, - - 2014
The localization of mRNA forms a key facet of the post-transcriptional control of gene expression and recent evidence suggests that it may be considerably more widespread than previously anticipated. For example, defined mRNA-containing granules can be associated with translational repression or activation. Furthermore, mRNA P-bodies (processing bodies) harbour much of ...
Bastos H B A HB REPROLAB-Faculdade de Veterinária, UFRGS, Porto Alegre-RS, Brazil. Electronic address: - - 2014
The period from the emergence of a dominant follicle until its formation requires tissue remodeling. Enzymes promoting collagen lysis, such as matrix metalloproteinases (MMPs), are fundamental for the process of extracellular matrix remodeling, which allows changes in ovarian tissue architecture during follicular growth. It has been suggested that the production ...
Rouvinen-Watt Kirsti K Department of Plant and Animal Sciences, Faculty of Agriculture, Dalhousie University, P.O. Box 550, Truro, NS, B2N 5E3, Canada, - - 2014
We investigated the presence of inflammatory signs in the progression of fatty liver disease induced by fasting. Sixty standard black American mink (Neovison vison) were fasted for 0, 1, 3, 5, or 7 days and one group for 7 days followed by re-feeding for 28 days. Liver sections were evaluated histologically and liver ...
Billman E J EJ Department of Fisheries and Wildlife, Oregon State University, 104 Nash Hall Corvallis, OR 97331, - - 2014
Body morphology of juvenile Chinook salmon Oncorhynchus tshawytscha in the upper Willamette River, Oregon, U.S.A., was analysed to determine if variation in body shape is correlated with migratory life-history tactics followed by juveniles. Body shape was compared between migrating juveniles that expressed different life-history tactics, i.e. autumn migrants and yearling ...
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