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Vencken Sebastian S Respiratory Research Division, Department of Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Beaumont Road, Dublin 9, - - 2015
Several experimental methods exist to explore the microRNA (miRNA) regulome. These methods almost exclusively focus on multiple targets bound to a single, or perhaps a few miRNAs of interest. Here, we describe a microRNA capture affinity technology (miR-CATCH) which uses an affinity capture oligonucleotide to co-purify a single target messenger ...
Maltby Steven S Priority Research Centre for Asthma and Respiratory Diseases, School of Biomedical Sciences and Pharmacy, Faculty of Health, University of Newcastle, Lot 1 Kookaburra Circuit, New Lambton Heights, NSW, 2305, Australia, - - 2015
MicroRNAs (miRNAs) are small noncoding RNA molecules that can modulate mRNA levels through RNA-induced silencing complex (RISC)-mediated degradation. Recognition of target mRNAs occurs through imperfect base pairing between an miRNA and its target, meaning that each miRNA can target a number of different mRNAs to modulate gene expression. miRNAs have ...
Saif Jaimy J *Bristol Heart Institute, School of Clinical Sciences, Bristol Royal Infirmary-Level 7, University of Bristol, Bristol BS2 8HW, - - 2014
miRNAs are highly conserved non-coding RNA molecules that negatively control gene expression by binding to target mRNAs promoting their degradation. A multitude of miRNAs have been reported to be involved in angiogenesis and vascular remodelling. In the present review, we aim to describe the effect of miRNAs in post-ischaemic repair. ...
Justus Georgia - - 2014
Abstract Background: The long-term outcomes of antenatal glucocorticoids (GCs) vary between reports, and have generated controversy in terms of repeated and single-course events, causing irreversible effects on endocrine set points. Aim: This study aimed to assess the effects of alternative therapeutic agents other than synthetic glucocorticoid GC administration for fetal ...
Jariwala Nidhi N Department of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, VA, - - 2014
The staphylococcal nuclease and tudor domain containing 1 (SND1) is a multifunctional protein overexpressed in breast, prostate, colorectal and hepatocellular carcinomas and malignant glioma. Molecular studies have revealed the multifaceted activities of SND1 involved in regulating gene expression at transcriptional as well as post-transcriptional levels. Early studies identified SND1 as a ...
Zhang Meiling M Southern Illinois University, United - - 2014
Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children. Many cellular disruptions contribute to the progression of this pediatric cancer including aberrant alternative splicing. The MEF2 family of transcription factors regulates many developmental programs, including myogenesis. MEF2 gene transcripts are subject to alternate splicing to generate protein isoforms ...
Zhao Xin X Department of Pharmacology, Harbin Medical University, Harbin, - - 2014
Arsenic trioxide (As2 O3 ) is used to treat acute promyelocytic leukaemia. However, the cardiotoxicity of long QT syndrome restricts its clinical application. Previous studies showed that As2 O3 can damage the hERG current via disturbing its trafficking to cellular membrane. Consistent with these findings, in this study we reported that ...
Shang Chao C Department of Neurobiology, China Medical University, No. 92 Beier Road, Heping District, Shenyang, 110001, People's Republic of - - 2014
To investigate whether miR-21 can affect the apoptosis and proliferation of glioblastoma cancer stem cells (GSCs) from down-regulating FASLG. The expression of miRNA-21 was detected by quantitative real-time PCR in normal brain tissue and glioblastoma samples, and the changes of miRNA-21 expression between GSCs and non-GSCs were also detected. The ...
Wang Bo - - 2014
BackgroundRecent studies observed that altered energy metabolism has become widespread in cancer cells along with other cancer-associated traits that have been accepted as hallmarks of cancer. Akt signaling pathway is involved in the aerobic glycolysis program. However, mechanisms underlying the regulation of aerobic glycolysis and Akt activity in gliomas remain ...
Montagner Giulia G Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, - - 2014
In the treatment of hemoglobinopathies, amending altered hemoglobins and/or globins produced in excess is an important part of therapeutic strategies and the selective inhibition of globin production may be clinically beneficial. Therefore the development of drug-based methods for the selective inhibition of globin accumulation is required. In this study, we ...
Shimazu Y Y Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, - - 2014
Reduced expression in immortalized cells/Dickkopf-3 (REIC/Dkk-3) was identified as a gene whose expression is reduced in many human cancers. REIC/Dkk-3 expression is also downregulated in malignant glioma and regulates cell growth through caspase-dependent apoptosis. cRGD (EMD121974), an antagonist of integrins, has demonstrated preclinical efficacy against malignant glioma. In this study, ...
Zhao Dong D Department of Orthopedics, Affiliated Hospital, Logistics University of Chinese People's Armed Police Forces, No. 220 Chenglin Road, Hedong district, Tianjin, 300162, China, - - 2014
MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression. The aberrant expression of miRNA has become a major focus in cancer research. This study aimed to investigate the importance of miR-410 in the diagnosis and therapy of osteosarcoma (OS). Western blot analysis showed that the expression of VEGF ...
Simpson Robert J RJ Diabetes and Nutritional Sciences, School of Medicine, Kings College London, 150 Stamford Street, London SE1 9NH, UK. - - 2014
Iron and oxygen metabolism are intimately linked with one another. A change in the level of either metabolite results in activation of common pathways. At the heart of these responses lies a group of iron and oxygen dependent enzymes called prolyl hydroxylases. Prolyl hydroxylases (PHDs) require both iron and oxygen ...
Arunachalam Gnanapragasam G 1Departments of Pharmacology and 2Medical Education, Weill Cornell Medical College in Qatar, P.O. Box: 24144, Education City, Doha, - - 2014
The worldwide increase in the prevalence of obesity and type 2 diabetes (T2DM) and the associated elevated risk of cardiovascular disease (CVD) has emphasised the need to seek new therapeutic targets to offset the negative impact on human health outcomes. In this regards microRNAs (miRNAs), a class of small noncoding ...
Lang Annemarie A Institute of Immunology, Department of Veterinary Medicine, Freie Universität Berlin, Germany; Department of Rheumatology and Clinical Immunology, Charité University Hospital, Berlin, Germany; German Rheumatism Research Center, Berlin, Germany; Berlin-Brandenburg School of Regenerative Therapies, Charité University Hospital, Berlin, - - 2014
Gene therapy seems promising to achieve long-term remedy for osteoarthritis (OA). However, there are several risks of adverse reactions especially when using CMV-controlled expression. To provide a safe application, we focused on the expression of therapeutic cytokines, e.g. IL-4, in a disease-responsive manner by use of the previously cloned Cox-2 ...
Arfat Yasir - - 2014
Advanced studies of single stranded endogenous ~22 nt microRNAs (miRNAs) have demonstrated their diverse biological functions including control of cell differentiation, cell cycle and pathological conditions. Recent studies suggest the potential application of miRNAs in stem cell engineering. miRNAs play a vital role as post-transcriptional regulators of gene expression which ...
Zhao Boxuan Simen BS Department of Chemistry and Institute for Biophysical Dynamics, Howard Hughes Medical Institute, 929 East 57th Street, The University of Chicago, Chicago, IL, 60637, - - 2014
Two articles recently published in Nature and Cell report the first transcriptome-wide maps of pseudouridine (Ψ) at single-base resolution through selective chemical labeling, suggesting new mechanisms and functions of Ψ in mRNA and non-coding RNA molecules.
Li Jarod J University of Cape Town, South - - 2014
A growing body of work has shown that the highly homologous T-box transcription factors TBX2 and TBX3 play critical but distinct roles in embryonic development and cancer progression. For example, TBX2 and TBX3 are upregulated in several cancers and recent evidence suggests that whereas TBX2 functions as a pro-proliferative factor, ...
Wen Luan L Section on Molecular Morphogenesis, Program in Cellular Regulation and Metabolism, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), U.S. National Institutes of Health, Bethesda, Maryland, USA; - - 2014
Histone methylations have been implicated to play important roles in diverse cellular processes. Of particular interest is the methylation of histone H3K79, which is catalyzed by an evolutionarily conserved methyltransferase, disruptor of telomeric silencing (Dot1)-like (Dot1L). To investigate the role of Dot1L during vertebrate development, we have generated a Dot1L-specific ...
Weissman Drew D Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, - - 2014
mRNA is the central molecule of all forms of life. It is generally accepted that current life on Earth descended from an RNA world. mRNA, after its first therapeutic description in 1992, has recently come into increased focus as a method to deliver genetic information. The recent solution to the ...
Wang Jian J Department of Neurosurgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, People's Republic of - - 2014
Substantial evidence indicates that the human ether-a-go-go-related gene potassium channel (hERG, Kv11.1, KCNH2) is overexpressed in human glioblastoma multiforme (GBM) specimens and plays an essential role in the malignant proliferation of glioma cells. However, its upstream regulator in glioma cells is not fully elucidated. The present study was designed to ...
Saeed Khalid K Institute for Molecular Medicine Finland, FIMM, University of Helsinki, - - 2014
Hormonal therapies targeting androgen receptor (AR) are effective in prostate cancer (PCa), but often the cancer progress to fatal castrate-resistant disease. Improved understanding of the cellular events during androgen-deprivation would help to identify survival and stress pathways whose inhibition could synergize with androgen-deprivation. Towards this aim, we performed an RNAi ...
Guo Jieming J Institute of Life and Health Engineering, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China. tongwang@jnu.edu.cn - - 2014
We previously found that shorter mRNAs are preferably translated in various eukaryotic cells. However, the theoretical basis of this phenomenon is unclear. We hypothesize that shorter mRNA length correlates to the decreased translational error rate to reduce the energy consumption on defective protein degradation. In this study, we established a ...
Bollmann Franziska F Department of Pharmacology, Johannes Gutenberg-University Medical Center, Mainz, - - 2014
Resveratrol shows beneficial effects in inflammation-based diseases like cancer, cardiovascular and chronic inflammatory diseases. Therefore, the molecular mechanisms of the anti-inflammatory resveratrol effects deserve more attention. In human epithelial DLD-1 and monocytic Mono Mac 6 cells resveratrol decreased the expression of iNOS, IL-8 and TNF-α by reducing mRNA stability without ...
Hu Yufeng Y School of Pharmacy, Huazhong University of Science and - - 2014
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive and lethal disease with a high rate of metastasis. Numerous signaling events have been implicated in the molecular pathogenesis of this neoplasm. Aberrantly high expression of Jagged2, one of the Notch ligands, often occurs in human PDAC. However, what role Jagged2 plays in ...
Geib Scott M - - 2014
The oriental fruit fly, Bactrocera dorsalis, is an important pest of fruit and vegetable crops throughout Asia, and is considered a high risk pest for establishment in the mainland United States. It is a member of the family Tephritidae, which are the most agriculturally important family of flies, and can ...
Li Huazhuang H Department of Orthopedics, Yidu Central Hospital, Weifang Medical College, Weifang, Shandong 262500, P.R. - - 2014
Osteosarcoma is the most common histological form of primary bone cancer, which arises from osteoid tissue. It occurs predominantly in infants and adolescents, with an incidence of 4‑5 cases/100,000,000. The 5-year survival rate of patients with osteosarcoma has significantly improved over time; however, there remains a significant proportion of patients that ...
Scieglinska Dorota D Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeże Armii Krajowej 15, 44-101, Gliwice, Poland, - - 2014
The HSPA2 gene is a poorly characterized member of the HSPA (HSP70) family. HSPA2 was originally described as testis-specific and expressed at the highest level in pachytene spermatocytes of rodents, the expression of which is not induced by heat shock. HSPA2 is crucial for male fertility. However, recent advances have ...
Boye Alex - - 2014
MicroRNAs are evolutionary conserved single stranded non-coding RNAs with immense ability to post-transcriptionally regulate gene expression via complementary base pairing with mRNAs of more than 50% protein encoding genes. They play diverse roles in physiological and pathophysiological processes such as normal development and cancer pathogenesis respectively. As a result, recent ...
Martinho Cláudia C Plant Stress Signaling, Instituto Gulbenkian de Ciência, Rua da Quinta Grande - 6, 2780-156 Oeiras, Portugal Present address: Sainsbury Laboratory, Cambridge University, Bateman Street, Cambridge, CB2 1LR, - - 2014
microRNAs (miRNAs) control gene expression mostly post-transcriptionally by guiding transcript cleavage and/or translational repression of complementary mRNA targets, thereby regulating developmental processes and stress responses. Despite the remarkable expansion of the field, the mechanisms underlying miRNA activity are not fully understood. In this paper, we describe a transient expression system ...
Pang Wenshuai - - 2014
Pim-1 kinase is a proto-oncogene and its dysregulation contributes to tumorigenesis and progression of a variety of malignancies. Pim-1 was suggested as a therapeutic target of cancers. The functional relevance of Pim-1 and the mechanism underlying its dysregulation in lung tumorigenesis remained unclear. This study aimed to investigate if Pim-1 ...
Hu Dong Gui DG Department of Clinical Pharmacology and Flinders Centre for Innovation in Cancer, Flinders University School of Medicine, Flinders Medical Centre , Bedford Park, SA , - - 2014
Abstract Glucuronidation is an important metabolic pathway for many small endogenous and exogenous lipophilic compounds, including bilirubin, steroid hormones, bile acids, carcinogens and therapeutic drugs. Glucuronidation is primarily catalyzed by the UDP-glucuronosyltransferase (UGT) 1A and two subfamilies, including nine functional UGT1A enzymes (1A1, 1A3-1A10) and 10 functional UGT2 enzymes (2A1, ...
Hart Gerald W GW Johns Hopkins University School of Medicine, United States - - 2014
The dynamic cycling of N-acetylglucosamine (termed O-GlcNAcylation) on serine or threonine residues of nuclear or cytoplasmic proteins serves as a nutrient sensor, both independently and also via its interplay with other post-translational modifications, to regulate signaling, transcription and cell physiology. Emerging evidence suggests that dysregulation of this ubiquitous post-translational modification ...
Long Mark D MD Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Elm & Carlton Streets, Buffalo, NY, 14263, - - 2014
Following the elucidation of the human genome and components of the epigenome, it is timely to revisit what is known of vitamin D receptor (VDR) function. Early transcriptomic studies using microarray approaches focused on the protein coding mRNA that were regulated by the VDR, usually following treatment with ligand. These ...
Petrilli Raquel - - 2014
Antisense oligonucleotides (AS-ODNs) are short, single-stranded DNA molecules designed to bind specifically to a target messenger RNA (mRNA) and down-regulate gene expression. Despite being a promising class of therapeutics for a variety of diseases, they face major hurdles limiting their clinical application, including low intracellular delivery and poor in vivo ...
Alenghat Theresa T Division of Immunobiology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA - - 2014
The mammalian gastrointestinal tract is home to trillions of commensal microorganisms that collectively make up the intestinal microbiota. These microbes are important environmental factors that regulate homeostasis, and alterations in the composition of the microbiota have been associated with several diseases, including inflammatory bowel disease, diabetes, and cancer. New research ...
Lamy Sylvie S Laboratoire d'Oncologie Moléculaire, Centre de Recherche BioMed, Université du Québec à Montréal, C.P. 8888, Succ. Centre-ville, Montréal, Québec H3C 3P8, Canada. Electronic address: - - 2014
Success in developing therapeutic approaches to target brain tumor-associated inflammation in patients has been limited. Given that the inflammatory microenvironment is a hallmark signature of solid tumor development, anti-inflammatory targeting strategies have been envisioned as preventing glioblastoma initiation or progression. Consumption of foods from plant origin is associated with reduced ...
Brüning Ansgar A Department of Obstetrics/Gynecology, Molecular Biology Laboratory, University Hospital Munich, Maistrasse 11, 80337, Munich, Germany, - - 2014
The family of metastasis-associated (MTA) genes is a small group of transcriptional co-regulators which are involved in various physiological functions, ranging from lymphopoietic cell differentiation to the development and maintenance of epithelial cell adhesions. By recruiting histone-modifying enzymes to specific promoter sequences, MTA proteins can function both as transcriptional repressors ...
Wei Jiali J School of Life Sciences, Shanghai University, Shanghai 200444, P.R. - - 2014
microRNAs (miRNAs) are small, non‑coding RNAs involved in multiple biological pathways by regulating post‑transcriptional gene expression. Previously, autophagy has been reported to suppress the progression of non‑small cell lung cancer (NSCLC). However, how miRNAs regulate autophagy in NSCLC remains to be elucidated. In the present study, the autophagy gene, autophagy‑related 2B ...
Kosztyu Petr P Department of Biology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hnevotinska 3, Olomouc, 77515, Czech - - 2014
Increased expression of the ABCB1 gene in cancer cells is usually connected with occurrence of multidrug resistance (MDR) and poor prognosis. However, the correlation between ABCB1 expression and MDR phenotype is difficult to prove in clinical samples. Most of the researchers believe that these difficulties are due to the poor ...
Lück Sarah S Institute for Theoretical Biology, Charité-Universitätsmedizin, 10115 Berlin, - - 2014
The rich mammalian cellular circadian output affects thousands of genes in many cell types and has been the subject of genome-wide transcriptome and proteome studies. The results have been enigmatic because transcript peak abundances do not always follow the peaks of gene-expression activity in time. We posited that circadian degradation ...
Tuure L L The Immunopharmacology Research Group, University of Tampere School of Medicine and Tampere University Hospital , Tampere , - - 2014
Objectives: Microsomal prostaglandin E synthase-1 (mPGES-1) is a terminal enzyme in the production of prostaglandin E2 (PGE2) and its expression is upregulated during inflammation. mPGES-1 is considered as a potential drug target for the treatment of arthritis to reduce adverse effects related to the current non-steroidal anti-inflammatory drugs (NSAIDs). Our ...
Standaert Laura L Center for the Biology of Disease, Laboratory for Molecular Cancer Biology, VIB, Leuven 3000, Belgium Center for Human Genetics, Laboratory for Molecular Cancer Biology, KULeuven, Leuven 3000, - - 2014
The lncRNA Neat1 is an essential architectural component of paraspeckle nuclear bodies. Although cell-based studies identified Neat1-paraspeckles as key regulators of gene expression through retention of hyperdited mRNAs and/or transcription factors, it is unclear under which specific physiological conditions paraspeckles are formed in vivo and whether they have any biological ...
Zhao Lianggong L The Second Clinical Medical College of Lanzhou University, 82 Cuiying Men, Lanzhou 730030, PR China; Orthopaedics Key Laboratory of Gansu Province, Lanzhou 730000, PR - - 2014
Radix Hedysari polysaccharides (HPS) is the principal active fraction of Radix Hedysari (RH). The information about HPS3d, the main fraction of HPS3, and its effect on bone is still unknown. In the present study, the purified HPS3d was obtained by anion-exchange column. It consisted of 94.38% polysaccharide, 3.40% protein and ...
Tay Hock L HL Priority Research Centre for Asthma and Respiratory Disease, Department of Microbiology and Immunology, School of Pharmacy and Biomedical Sciences, Faculty of Health and Hunter Medical Research Institute, University of Newcastle , Newcastle 2308 , - - 2014
MicroRNAs are small non-coding RNAs that bind to multiple target mRNAs to control gene expression post-transcriptionally by inhibiting translation. In mammalian cells, microRNAs play important roles in a diverse array of cellular processes (e.g. cell proliferation and differentiation). However, alterations in their levels may compromise cellular function, predisposing to disease. ...
Hardman Jonathan A JA 1] The Centre for Dermatology Research, Institute of Inflammation and Repair, University of Manchester, Manchester, UK [2] Doctoral Training Centre in Integrative Systems Biology, Manchester Institute of biotechnology, University of Manchester, Manchester, - - 2014
While the regulation of pigmentation is well-characterized it remains unclear whether cell-autonomous controls regulate the cyclic on-off switching of pigmentation in the hair follicle (HF). Since human HFs and epidermal melanocytes express clock genes and proteins, and given that core clock genes (PER1, BMAL1) modulate human HF cycling, we investigated ...
Aras Siddhesh S Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI 48201 - - 2014
Our understanding of stress-associated regulatory mechanisms for mitochondria remains incomplete. We now report a new regulator of mitochondrial metabolism, the coiled-coil-helix-coiled-coil-helix domain-containing protein 2 (CHCHD2) which, based on the functionality described here, is renamed MNRR1 (Mitochondria Nuclear Retrograde Regulator 1). It functions in a novel way by acting in two ...
Kojima Shihoko - - 2014
In order to maintain daily cycles, the circadian clock must tightly regulate the rhythms of thousands of mRNAs and proteins with the correct period, phase, and amplitude to ultimately drive the wide range of rhythmic biological processes. Recent genomic approaches have revolutionized our view of circadian gene expression and highlighted ...
Gustafson Chelsea L - - 2014
Mammalian circadian timekeeping arises from a transcription-based feedback loop driven by a set of dedicated clock proteins. At its core, the heterodimeric transcription factor CLOCK:BMAL1 activates expression of Period, Cryptochrome and Rev-Erb genes, which feed back to repress transcription and create oscillations in gene expression that confer circadian timing cues ...
Hapangama D K DK Department of Women's and Children's Health, Institute of Translational Medicine, University of Liverpool, Liverpool Women's NHS Foundation Trust, Liverpool L8 7SS, UK - - 2014
The endometrium is the primary target organ for the 'female' sex steroid hormone estrogen, which exerts effects in the endometrium via two main classical estrogen receptor (ER) isoforms, ERα and ERβ. The main function of the endometrium, embryo implantation, appears unperturbed in ERβ knockout mice, which has led researchers to ...
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