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Zhao Lianggong L The Second Clinical Medical College of Lanzhou University, 82 Cuiying Men, Lanzhou 730030, PR China; Orthopaedics Key Laboratory of Gansu Province, Lanzhou 730000, PR - - 2014
Radix Hedysari polysaccharides (HPS) is the principal active fraction of Radix Hedysari (RH). The information about HPS3d, the main fraction of HPS3, and its effect on bone is still unknown. In the present study, the purified HPS3d was obtained by anion-exchange column. It consisted of 94.38% polysaccharide, 3.40% protein and ...
Liu Bo B FIRST, Aihara Innovative Mathematical Modelling Project, Japan Science and Technology Agency, Tokyo, Japan Institute of Industrial Science, The University of Tokyo, Tokyo, Japan Key Laboratory of Intelligent Perception and Image Understanding of Ministry of Education of China, Xidian University, Xi'an, People's Republic of - - 2014
Gene transcription is a noisy process carried out by the transcription machinery recruited to the promoter. Noise reduction is a fundamental requirement for reliable transcriptional responses which in turn are crucial for signal transduction. Compared with the relatively simple transcription initiation in prokaryotes, eukaryotic transcription is more complex partially owing ...
Shridhar Varsha - - 2014
The CD8 Antiviral Factor (CAF) suppresses viral transcription from the HIV-1 Long Terminal Repeat (LTR) promoter in a non-cytolytic manner. However, the region on the LTR upon which CAF acts is unknown. Our objective was to determine the region on the LTR upon which CAF acts to suppress HIV-1 transcription. ...
Yamamoto Kaneyoshi K Department of Frontier Bioscience, Hosei University, - - 2014
Expression pattern of the genome in Escherichia coli is controlled by regulating the utilization of a limited number of RNA polymerase between a total of 4,600 genes on its genome. The distribution pattern of RNA polymerase on the genome changes after two steps of protein-protein interaction with 7 sigma subunits ...
Liu Xuewei X R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China; Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of - - 2014
There has been little research reported on the synergism of plant expansins in cellulose hydrolysis because of the difficulty of heterologous expression. In this study, the expansin gene LeExp2 of Lycopersicum esculentum was expressed for the first time in Pichia pastoris to identify its function in vitro. The optimal expression ...
Huang Xiaobo X Department of Chinese Medicine, Xuanwu Hospital, Capital Medical University , Beijing , China - - 2014
Abstract Context: DTD is a Chinese herb prescription used for centuries to treat atherosclerosis or dizziness. Previous studies show that DTD could inhibit ICAM-1 expression induced by TNF-α. However, its mechanism has never been clearly described. Objective: To examine the hypothesis that DTD might inhibit TNF-α-induced ICAM-1 expression through regulating ...
Wang Chen-Hsu CH Medical Intensive Care Unit, Cathay General Hospital , Taipei , Taiwan - - 2014
Abstract Background: Carbamazepine, which was developed primarily for the treatment of epilepsy, is now also useful for the treatment of non-epileptic disorders and inflammatory hyperalgesia. However, the mechanism of its anti-neuroinflammatory action remains poorly understood. Objective: This study elucidates the anti-neuroinflammatory capacity of carbamazepine on microglial activation and the relative ...
Domitrović Robert R Department of Chemistry and Biochemistry, Medical Faculty, University of Rijeka, B. Branchetta 20, 51000 Rijeka, Croatia. Electronic address: - - 2014
The aim of this study was to investigate the renoprotective activity of chlorogenic acid (CA) in a murine model of cisplatin (CP)-induced kidney injury. Male BALB/cN mice were gavaged daily with CA at 3, 10 and 30mg/kg for two successive days, 48h after intraperitoneal injection of CP (13mg/kg). On the ...
Pathak R R Division of Radiation Health, Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, Little Rock, - - 2014
Endothelial thrombomodulin (TM) is critically involved in anti-coagulation, anti-inflammation, cytoprotection, and normal fetal development. Tumor necrosis factor alpha (TNF) suppresses TM expression. TNFα has been shown to down-regulate TM partly via activation of nuclear factor kappa B (NF-B). However, because the TM promoter lacks an NF-κB binding site, the direct ...
Kasperova Alena A Department of Immunology, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Olomouc, Czech - - 2014
Cysteine dioxygenase (CDO) is involved in regulation of intracellular cysteine levels by catabolising the cysteine to sulphite and sulphate. In keratinolytic fungi, sulphite is actively excreted to reduce disulphide bridges in keratin before its enzymatic degradation. The pathogenicity role of CDO was confirmed in cysteine-hypersensitive and growth-defective ΔCdo mutant of ...
Zhao Shengqiang S Center for Vascular Biology Research, Division of Molecular and Vascular Biology, and Division of Gastroenterology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA;Center for Vascular Biology Research, Division of Molecular and Vascular Biology, and Division of Gastroenterology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA; Department of Gastroenterology, Provincial Hospital Affiliated to Shandong University, Ji-nan, - - 2014
Angiogenesis is a hallmark of many diseases, including cancer, ischemic heart disease, inflammation, and others. It is well known that vascular endothelial growth factor (VEGF) is the most important angiogenic factor. Recently, we demonstrated that orphan nuclear receptor TR3 (mouse Nur77 and rat NGFI-B) plays critical roles in tumor growth ...
Burns Michael L ML Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, - - 2014
Brain-derived neurotrophic factor (BDNF) plays an important role in nervous system function and has therapeutic potential. Microbial production of BDNF has resulted in low fidelity protein product, often in the form of large insoluble aggregates incapable of binding to cognate TrkB or p75 receptors. In this study, employing yeast display ...
Xia Ning - - 2014
Resveratrol (3,5,4'-trihydroxy-trans-stilbene) possesses multiple protective properties in the vasculature, including anti-oxidative and anti-inflammatory effects and improvement of endothelial function. A substantial part of these effects is attributable to gene expression changes induced by the compound. Resveratrol can activate the NAD-dependent deacetylase sirtuin 1 (SIRT1), leading to deacetylation of SIRT1 target ...
Chen Ming M Thomas Jefferson University, United - - 2014
Protein arginine methyltransferase 5 (PRMT5), a protein arginine methyltransferase that catalyzes the symmetrical dimethylation of arginine residues within target proteins, has been implicated in many essential cellular processes, ranging from the regulation of gene expression to cell proliferation and differentiation. PRMT5 is highly expressed in the heart, the functional role ...
Du Minmin M State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China College of Horticulture, Northeast Agricultural University, Harbin 150030, - - 2014
To restrict pathogen entry, plants close stomata as an integral part of innate immunity. To counteract this defense, Pseudomonas syringae pv tomato produces coronatine (COR), which mimics jasmonic acid (JA), to reopen stomata for bacterial entry. It is believed that abscisic acid (ABA) plays a central role in regulating bacteria-triggered ...
Templeton Neil N Department of Chemical and Biomolecular Engineering, Vanderbilt University; PMB 351604, 2301 Vanderbilt Place, Nashville, TN, 37235-1604, - - 2014
Anti-apoptosis engineering is an established technique to prolong the viability of mammalian cell cultures used for industrial production of recombinant proteins. However, the effect of overexpressing anti-apoptotic proteins on central carbon metabolism has not been systematically studied. We transfected CHO-S cells to express Bcl-2∆, an engineered anti-apoptotic gene, and selected ...
Iyer Srividhya S University of Arkansas for Medical Sciences, United - - 2014
A decline of the levels and activity of Sirtuin1 (Sirt1), a NAD+ class III histone deacetylase, with age contributes to the development of several diseases including type 2 diabetes, neurodegeneration, inflammation, and cancer. The anti-aging effects of Sirt1 evidently result from the deacetylation of many transcription factors and co-factors including ...
Hong Sohyun S Department of Life Science and the Research Center for Cellular Homeostasis, Ewha Womans University, Seoul 120-750, - - 2014
We previously reported the inhibitory role of thioredoxin-related protein of 14 kDa (TRP14), a novel disulfide reductase in nuclear factor-κB (NF-κB) activation, but its biological function has remained to be explored. Here, we evaluated the role of TRP14 in the differentiation and function of osteoclast (OC), for which NF-κB and ...
Ehira Shigeki S Department of Biological Sciences, Graduate school of Science and Engineering, Tokyo Metropolitan University, Hachioji, Tokyo, JapanDepartment of Biological Science, Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo, Japan Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan - - 2014
The filamentous, nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 accumulates sucrose as a compatible solute against salt stress. Sucrose-phosphate synthase activity, which is responsible for the sucrose synthesis, is increased by salt stress, but the mechanism underlying the regulation of sucrose synthesis remains unknown. In the present study, a response ...
Ing Nancy H NH Department of Animal Science, Texas A&M AgriLife Research. Electronic address: - - 2014
In rodents, livestock and primate species, a single dose of the synthetic glucocorticoid dexamethasone acutely lowers testosterone biosynthesis. To determine the mechanism of decreased testosterone biosynthesis, stallions were treated with 0.1mg/kg dexamethasone 12h prior to castration. Dexamethasone decreased serum concentrations of testosterone by 60% compared to saline-treated control stallions. Transcriptome ...
Yadav Poonam P a National Bureau of Animal Genetic Resources , Karnal , - - 2014
Lactation is an important physiological process in dairy animals. During lactation, up to 85% of the body glucose is directed toward the mammary glands for milk synthesis. Studies related to lactation physiology are generally carried out on mammary biopsies, which may adversely affect animal health. In the present study, milk ...
Wright P W PW Basic Science Program, Leidos Biomedical Research Inc., Lab of Experimental Immunology, Frederick National Lab, Frederick, MD, - - 2014
Members of the human KIR (killer cell immunoglobulin-like receptor) class I major histocompatibility complex receptor gene family contain multiple promoters that determine the variegated expression of KIR on natural killer cells. In order to identify novel genetic alterations associated with decreased KIR expression, a group of donors was characterized for ...
Zhou Xiaoming X Uniformed Services University of the Health Sciences - - 2014
Visceral leishmaniasis patients have been reported to have a urine concentration defect. Concentration of urine by the renal inner medulla is essentially dependent on a transcription factor, NFAT5/TonEBP, because it activates expression of osmoprotective genes BGT1 and SMIT, and water channel aquaporin-2, all of which are imperative for concentrating urine. ...
Lu Jing-Jing JJ State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, - - 2014
In response to deprivation of combined nitrogen, the filamentous cyanobacterium Anabaena sp. strain PCC 7120 develops heterocyst, which is specifically involved in the nitrogen fixation. In this study, we focused on the regulation of HanA, a histone-like protein, in heterocyst development. Electrophoretic mobility shift assay results showed that NtcA, a ...
Li Yan Y The MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, - - 2014
The type III secretion system (T3SS) is a major virulence factor in many Gram-negative bacterial pathogens and represents a particularly appealing target for antimicrobial agents. Previous studies have shown that the plant phenolic compound p-coumaric acid (PCA) plays a role in inhibiting the T3SS expression of the phytopathogen D. dadantii ...
Clayton C E CE Universität Heidelberg, Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Alliance, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany. Electronic address: - - 2014
Regulation of gene expression in Kinetoplastids relies mainly on post-transcriptional mechanisms. Recent high-throughput analyses, combined with mathematical modelling, have demonstrated possibilities for transcript-specific regulation at every stage: trans splicing, polyadenylation, translation, and degradation of both the precursor and the mature mRNA. Different mRNA degradation pathways result in different types of ...
Verma Amit Kumar AK Molecular Biophysics Unit, Indian Institute of Science, - - 2014
MsRbpA is a RNA polymerase (RNAP) binding protein from Mycobacterium smegmatis. According to our earlier studies, MsRbpA rescues rifampicin induced transcription inhibition upon binding to the RNAP. Hu et al., (2012) showed that RbpA from Mycobacterium tuberculosis (MtbRbpA) is a transcription activator. In this study, we report that both MsRbpA ...
Pillai-Kastoori Lakshmi L Department of Biology, University of Kentucky, Lexington, Kentucky, United States of - - 2014
Ocular coloboma is a sight-threatening malformation caused by failure of the choroid fissure to close during morphogenesis of the eye, and is frequently associated with additional anomalies, including microphthalmia and cataracts. Although Hedgehog signaling is known to play a critical role in choroid fissure closure, genetic regulation of this pathway ...
Chahar Sanjay S Human Genetics Institute of New Jersey and the Department of Genetics, Rutgers, the State University of New Jersey, Piscataway, - - 2014
Transcriptional regulatory mechanisms likely contribute to the etiology of Inflammatory Bowel Disease (IBD), as genetic variants associated with the disease are disproportionately found at regulatory elements. However, the transcription factors regulating colonic inflammation are unclear. To identify these transcription factors, we mapped epigenomic changes in the colonic epithelium upon inflammation. ...
Joshi Shweta S University of California San Diego, United - - 2014
Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor containing an inducibly expressed HIF1α subunit and a constitutively expressed HIF1β subunit. Under hypoxic conditions, the HIF1α subunit accumulates due to a decrease in the rate of proteolytic degradation, and the resulting HIF1α-HIF1β heterodimers undergo posttranslational modifications that promote transactivation. Previous ...
Chauhan Jay J Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, 20 North Pine Street, Baltimore, MD 21201 (USA), Fax: - - 2014
c-Myc is a basic helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor that is responsible for the transcription of a wide range of target genes involved in many cancer-related cellular processes. Over-expression of c-Myc has been observed in, and directly contributes to, a variety of human cancers including those of the hematopoietic system, ...
Hancock R R University of Oxford, Department of Chemistry, Oxford, United - - 2014
JmjC (Jumonji C)-domain-containing human histone-lysine demethylase (KDM) enzymes are Fe(II) and 2-oxoglutarate dependent oxygenases involved in epigenetic regulation. The KDM4 sub-family demethylate di-and tri-methylated lysine residues 9 and 36 on Histone 3 (H3K9me2/3 and H3K36me2/3, respectively) regulating transcription (1). Increased methylation of these histone markers in macrophages under hypoxia, a ...
Xu Hui H College of Food Science and Engineering, Ocean University of China, No. 5 Yu Shan Road, Qingdao, Shandong Province 266003, - - 2014
The fucosylated chondroitin sulfate was isolated from the sea cucumber Acaudina molpadioides (Am-CHS) and its effect on the adipogenesis was investigated in vitro. Results showed that Am-CHS exhibited a marked anti-adipogenic effect. The TG contents of cells treated with Am-CHS from the early stage, mid stage and later stage were ...
Solomon Laura L Biology, Villanova University, Villanova, PA, - - 2014
The bacterial ydcI gene encodes a highly conserved transcriptional regulatory protein found in a wide range of Gram-negative bacteria and is involved in a number of Salmonella enterica serovar Typhimurium phenotypes. Given its high conservation, the YdcI protein has the potential for studies and applications across bacterial genera. However, no ...
Tsuzuki Masayuki M Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo 153-8902, - - 2014
MicroRNAs (miRNAs) act as down-regulators of gene expression, and play a dominant role in eukaryote development. In Arabidopsis thaliana, DICER-LIKE 1 (DCL1) is the main processor in miRNA biogenesis, and dcl1 mutants show various developmental defects at the early stage of embryogenesis or at gamete formation. However, miRNAs responsible for ...
Bartling Toni T College of Nanoscale Science and Engineering, State University of New York, Albany, NY 12203, - - 2014
Aberrant matrix metalloproteinase-1 (MMP-1) expression contributes to the pathogenesis of many degenerative disease processes that are associated with increased oxidative damage or stress. We and others have established that shifts in steady-state H2O2 production resulting from enforced antioxidant gene expression, senescence or UV irradiation control MMP-1 expression. Here we establish ...
Buhrmann Constanze C Ludwig-Maximilian-University Munich, - - 2014
Sirtuin-1 (SIRT1), a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase, has been linked to anabolic effects in cartilage, while the mechanisms of SIRT1 signaling during differentiation of mesenchymal stem cells (MSCs) to chondrocytes are poorly understood. Therefore, we investigated the role of SIRT1-mediated signaling during chondrogenic differentiation of MSCs in vitro. High-density ...
Kandalam Umadevi U Department of Pediatric Dentistry, Nova Southeastern University, - - 2014
Recently we established that pro-inflammatory actions of angiotensin (Ang) II in astrocytes involved Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), and interleukin-6 (IL-6). In our current study, we determined in brainstem and cerebellum whether Ang III also activates STAT3 leading to IL-6 mRNA expression and ...
Choi You Hee YH College of Pharmacy and Research Institute of Drug Development, Chonnam National University, 500-757, Gwangju, Republic of - - 2014
Runx2 plays essential roles in bone formation and chondrocyte maturation. Akt promotes BMP2-induced osteoblast differentiation and enhances the function and transcriptional activity of Runx2. However, the precise molecular mechanism underlying the relationship between Runx2 and Akt are not well understood. In this study, we examined the role of Akt in ...
Tsai Ming-Horng MH Department of Pediatrics, Division of Neonatology and Pediatric Hematology/Oncology, Chang Gung Memorial Hospital, Yunlin, - - 2014
Eupafolin, a major active component found in the methanol extracts of Phyla nodiflora, has been used to treat inflammation of skin. We examined its effects on cyclooxygenase-2 (COX-2) expression in LPS-treated human dermal fibroblasts. Lipopolysaccharide (LPS) significantly increased prostaglandin-E2 (PGE2) production associated with increased COX-2 expression in Hs68 cells. This ...
Ji Hong-Lei HL Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China, - - 2014
MicroRNAs are a family of small, non-coding RNAs that regulate gene expression in a sequence-specific manner. Estrogen-related receptor α (ERRα) is an orphan nuclear receptor which plays an important role in adipocyte differentiation. Our previous Solexa sequencing results indicated a high expression of miR-125a in adult pig backfat. In this ...
Ding Zhong Jie ZJ State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, - - 2014
Although seed dormancy is an important agronomic trait, its molecular basis is poorly understood. ABSCISIC ACID INSENSITIVE3 (ABI3) plays an essential role in establishment of seed dormancy. Here, we show that lack of a seed-expressed WRKY transcription factor, WRKY41, confers reduced primary seed dormancy and thermoinhibition, phenotypes resembling those by ...
Xiao Yuguo Y Institute of Biology, Leiden University, PO Box 9505, 2300 RA, Leiden, The - - 2014
Jasmonates are important phytohormones regulating reproductive development. We used two recessive rice Tos17 alleles of OsJAR1, osjar1-2 and osjar1-3, to study the biological function of jasmonates in rice anthesis. The florets of both osjar1 alleles stayed open during anthesis because the lodicules, which control flower opening in rice, were not ...
Wang Hecheng H Neuroscience Research Institute & Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education & Ministry of Public Health, Health Science Center, Peking University, Beijing, 100191, - - 2014
Hypoxia-inducible factor (HIF)-1 is the key transcriptional activator mediating both adaptive and pathological responses to hypoxia. The purpose of this study was to find the role of HIF-1 in regulating neprilysin (NEP) at the early stage of hypoxia and explore the underlying mechanism. In the present study, we demonstrate that ...
Pachiadaki Maria G MG Woods Hole Oceanographic Institution, Woods Hole, MA, - - 2014
Deep-sea hypersaline anoxic basins (DHABs) in the Eastern Mediterranean Sea are considered some of the most hostile environments on Earth. Little is known about the biochemical adaptations of microorganisms living in these habitats. This first metatranscriptome analysis of DHAB samples provides significant insights into shifts in metabolic activities of microorganisms ...
Tang Guirong G Shanghai Key Lab of Bio-energy Plant, School of Life Sciences, Shanghai University, Shanghai, 200444, China. State Key Lab of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China. University of the Chinese Academy of Sciences, Beijing 100049, - - 2014
Rhizobia induce nitrogen-fixing nodules on host legumes, which is important in agriculture and ecology. Lipopolysaccharide (LPS) produced by rhizobia is required for infection or bacteroid survival in host cells. Genes required for LPS biosynthesis have been identified in several Rhizobium species. However, the regulation of their expression is not well ...
Huang Szu-Chuan SC Graduate Institute of Biomedical - - 2014
Urease gene expression in Streptococcus salivarius 57.I, one of the major alkali producers in the mouth, is induced by acidic pH and excess amounts of carbohydrate. Expression is controlled primarily at the transcriptional level from a promoter, pureI. Recent sequencing analysis revealed a CodY box located 2 bases 5' to ...
Ding Ze-Yang ZY Hepatic Surgery Centre, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, - - 2014
Transcriptional intermediary factor 1 γ (TIF1γ) may play either potential tumor suppressor or promoter role in cancer. Herein we reported a critical role of TIF1γ in the progression of hepatocellular carcinoma (HCC). Reduced expression of TIF1γ was detected in HCC, especially in advance HCC tissues compared with adjacent noncancerous tissues. ...
Lee Eun-Gyeong EG Department of Internal Medicine, Medical School and Research Institute of Clinical Medicine, Chonbuk National University and Chonbuk, National University Hospital, Jeonju, Jeonbuk 561-712, South - - 2014
The mechanism by which IL-1β and thapsigargin (TG)-induced endoplasmic reticulum (ER) stress modulate the receptor activator of nuclear factor kappa-B ligand (RANKL)-mediated osteoclastogenesis remains elusive. Thus, we investigated the osteoclast-specific and ER signals in osteoclastogenesis of bone marrow-derived cells. Bone marrow cells (BMCs) were obtained from 5-week-old male ICR mice ...
Lin Hai H Department of Otorhinolaryngology, Eye and ENT Hospital of Fudan University, 83 Fenyang Road, Xuhui District, Shanghai, 200031, - - 2014
The pathogenesis of human chronic rhinosinusitis (CRS) remains controversial. Recent evidence has suggested that caveolin-1 (Cav-1) is a 22 kDa scaffolding protein and plays a pivotal role in host defense against infections and tumour suppression by reducing production of cyclin D1 and endothelial nitric oxide-synthase (eNOS). However, little is known about ...
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