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Zhao Lianggong L The Second Clinical Medical College of Lanzhou University, 82 Cuiying Men, Lanzhou 730030, PR China; Orthopaedics Key Laboratory of Gansu Province, Lanzhou 730000, PR - - 2014
Radix Hedysari polysaccharides (HPS) is the principal active fraction of Radix Hedysari (RH). The information about HPS3d, the main fraction of HPS3, and its effect on bone is still unknown. In the present study, the purified HPS3d was obtained by anion-exchange column. It consisted of 94.38% polysaccharide, 3.40% protein and ...
Guan Fuqin F Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences/Nanjing Botanical Garden Memorial Sun Yat-Sen, Nanjing, Jiangsu 210014, P.R. China ; College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. - - 2014
Lonimacranthoide VI, first isolated from the flower buds of Lonicera macranthoides in our previous study, is a rare chlorogenic acid ester acylated at C-23 of hederagenin. In the present study, the anti-inflammatory effects of lonimacranthoide VI were studied. Lipopolysaccharides (LPS) induced an inflammatory response through the production of prostaglandin E2 ...
Wang Jingli J Auckland Cancer Society Research Centre, Private Bag 92019, The University of Auckland, Auckland, New - - 2014
SN30000 is a second-generation benzotriazine-N-oxide hypoxia-activated prodrug scheduled for clinical trial. Previously we showed that covalent binding of the hypoxia probe EF5 predicts metabolic activation of SN30000 in a panel of cancer cell lines under anoxia, suggesting that they are activated by the same reductases. However the identity of these ...
Saksi Jani J Research Programs Unit, Molecular Neurology, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland - - 2014
-Fatty acid-binding protein 4 (FABP4 or aP2 in mice) has been identified as a key regulator of core aspects of cardiometabolic disorders including lipotoxic endoplasmic reticulum (ER) stress in macrophages. A functional promoter polymorphism (rs77878271) of human FABP4 gene has been described resulting in reduced FABP4 transcription. -We investigated the ...
Doron Lior L Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva, - - 2014
The expression of the CO2 -fixation enzyme ribulose-bisphosphate carboxylase/oxygenase (Rubisco), which is affected by light, involves the cysteine-rich protein, bundle-sheath defective-2 (BSD2) that was originally identified in maize bundle-sheath cells. We identified the BSD2 orthologue in Chlamydomonas reinhardtii as a small protein (17 kDa) that is localized to the chloroplast. ...
Kao Cheng-Yen CY Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, - - 2014
Motility mediated by the flagella of Helicobacter pylori has been shown to be required for normal colonization and is thought to be important for the bacteria to move toward the gastric mucus in niches adjacent to the epithelium. Barnard et al. showed that CsrA appears to be necessary for full motility ...
Shi Jia-Xin JX Department of Respiratory Medicine, Lianyungang First People's Hospital, Affiliated Hospital of Xuzhou Medical College, Clinical Medical School of Nanjing Medical University, Lianyungang 222002, China. Electronic address: - - 2014
Glucocorticoids have been widely used in various inflammatory disorders, and the transcriptional repression of inflammatory mediators has been considered to be the main mechanism of action. However, a previous study showed that dexamethasone inhibited interleukin 8 (IL-8) expression by promoting IL-8 mRNA decay, which implies a posttranscriptional regulation. Nevertheless, by ...
Zhao Ya Y Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, - - 2014
Numerous studies have established important roles for microRNAs (miRNAs) in regulating gene expression. Here, we report that miRNAs also serve as a surveillance system to repress the expression of nonsense mRNAs that may produce harmful truncated proteins. Upon recognition of the premature termination codon by the translating ribosome, the downstream ...
Liu Bo B Department of Pharmacology and Systems Therapeutics, Box - - 2014
Messenger RNA decay in Bacillus subtilis is accomplished by a combination of exoribonucleases and endoribonucleases. Intermediates in the decay process have not been readily detectable, and previous studies on mRNA decay have used a handful of highly expressed transcripts as models. Here, we use RNA-Seq analysis to probe mRNA turnover ...
Zhao Xia X Department of Pharmacology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0636, - - 2014
Activation of RhoA, a low molecular-weight G-protein, plays an important role in protecting the heart against ischemic stress. Studies using non-cardiac cells demonstrate that the expression and subsequent secretion of the matricellular protein CCN1 is induced by GPCR agonists that activate RhoA. In this study we determined whether and how ...
Köster Tino T Molecular Cell Physiology, Bielefeld - - 2014
The hnRNP-like glycine-rich RNA-binding protein AtGRP7 regulates pre-mRNA splicing in Arabidopsis. Here we used small RNA-seq to show that AtGRP7 also affects the miRNA inventory. AtGRP7 overexpression caused a significant reduction in the level of 30 miRNAs and an increase for 14 miRNAs with a minimum log2 fold change of ...
Liu Bo B FIRST, Aihara Innovative Mathematical Modelling Project, Japan Science and Technology Agency, Tokyo, Japan Institute of Industrial Science, The University of Tokyo, Tokyo, Japan Key Laboratory of Intelligent Perception and Image Understanding of Ministry of Education of China, Xidian University, Xi'an, People's Republic of - - 2014
Gene transcription is a noisy process carried out by the transcription machinery recruited to the promoter. Noise reduction is a fundamental requirement for reliable transcriptional responses which in turn are crucial for signal transduction. Compared with the relatively simple transcription initiation in prokaryotes, eukaryotic transcription is more complex partially owing ...
Sumi Daigo D Laboratory of Molecular Nutrition and Toxicology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima, 770-8514, - - 2014
Arsenic-induced toxicity appears to be dependent on the tissue- or cell-specific accumulation of this metalloid. An early study showed that arsenic was retained in the esophagus as well as the liver, kidney cortex and skin of marmosets after intraperitoneal administration of (74)As-arsenite. However, there is little available information regarding the ...
Ba Liang-Jie LJ State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, - - 2014
The ripening-induced MaBSD1 acts as a transcriptional activator, and might be involved in banana fruit ripening partly through directly activating the expression of two ripening-associated genes, MaEXP1/2. BSD (BTF2-like transcription factors, synapse-associated proteins and DOS2-like proteins) transcription factors are characterized by a typical BSD domain. However, little information is available ...
Chinenov Yurii - - 2014
Inflammation triggered by infection or injury is tightly controlled by glucocorticoid hormones which signal via a dedicated transcription factor, the Glucocorticoid Receptor (GR), to regulate hundreds of genes. However, the hierarchy of transcriptional responses to GR activation and the molecular basis of their oftentimes non-linear dynamics are not understood. We ...
Bianchi A A UMR 7365 CNRS-Université de Lorraine « Ingénierie Moléculaire et Physiopathologie Articulaire » (IMoPA), Biopôle de l'Université de Lorraine, Campus Biologie-Santé, Vandœuvre-lès-Nancy, France. Electronic address: - - 2014
The inhibitory effect of 15-deoxy-Δ(12, 14)-prostaglandin J2 (15d-PGJ2) on pro-inflammatory genes expression has been extensively documented and frequently ascribed to its ability to prevent NF-κB pathway activation. We and others have previously demonstrated that it was frequently independent of the peroxisome proliferator activated receptor (PPAR)γ activation. Here, we provide evidence ...
Kim Samin S Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH - - 2014
High-throughput RNA-sequencing technology has identified the 5' UTR region of sarA to contain two putative small RNAs, designated teg49and teg48. Northern blot analysis disclosed that teg49 and teg48 were detectable within the P3-P1 and P1 sarA promoter region, respectively. Focusing on teg49, we found that this small RNA, consisting of ...
Tan Shuwen S College of Life Science, Foshan University, No. 1 Xianhu University Road, Nanhai, Foshan, Guangdong 528231, China; Holdone Aquaculture Breeding Limited Company, Foshan, Guangdong 528231, - - 2014
To gain more knowledge about the physiological regulation of metal pollutant detoxification in grass carp, we examined Cd concentration and its the potential influence on the expression of metallothionein 2 (MT2) and multidrug resistance protein 2 (ABCC2) mRNA in the liver and kidney, using in vitro and in vivo experiments. ...
Choi Sung-Chan SC Department of Environmental Sciences and Biotechnology, Hallym University, Chunchon, 200-702, Republic of - - 2014
4-Hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF), a nonhalogenated furanone found in a variety of fruits, has been shown to have antimicrobial activity. However, few studies have focused on its inhibitory effect on bacterial quorum sensing (QS) at levels below the non-inhibitory concentration. In this study, 0.1 μM HDMF decreased the production of QS signal ...
Yauk Yar-Khing YK The New Zealand Institute for Plant & Food Research Limited (PFR), Private Bag 92169, Auckland, 1142, New - - 2014
Glycosides are an important potential source of aroma and flavour compounds for release as volatiles in flowers and fruit. The production of glycosides is catalysed by UDP-glycosyltransferases (UGTs) that mediate the transfer of an activated nucleotide sugar to acceptor aglycones. A screen of UGTs expressed in kiwifruit (Actinidia deliciosa) identified ...
Anwar-Mohamed Anwar A Faculty of Pharmacy and Pharmaceutical Sciences, 2142J Katz Group-Rexall Centre for Pharmacy and Health Research, University of Alberta, Edmonton, AB T6G 2E1, - - 2014
The toxic effects of arsenic on the whole brain, as well as the discrete regions, has been previously reported for mice. We investigated the effects of acute arsenite (As(III)) on brain levels of arachidonic acid (AA) and its associated metabolites generated through cytochrome P450 (CYP), cyclooxygenase (COX), and lipoxygenase (LOX) ...
Umasuthan Navaneethaiyer N Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea; Fish Vaccine Development Center, Jeju National University, Jeju Self-Governing Province 690-756, Republic of - - 2014
Superoxide dismutases (SODs) are dedicated to scavenge and dismutate the superoxide anions in order to protect the cells from oxidative stress by establishing the redox homeostasis. In this study, we describe a cytosolic Cu/ZnSOD, the second SOD member from rock bream Oplegnathus fasciatus (Of-cCu/ZnSOD) at molecular, genomic structural-, transcriptional- and ...
Abed Nadia N INRA, UMR1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France; Université François Rabelais de Tours, UMR1282 Infectiologie et Santé Publique, F-37000 Tours, - - 2014
One important step for the pathogenesis of Salmonella is its ability to penetrate host cells. Recently, a new entry system involving the outer membrane protein Rck has been characterized. Previous studies have shown that the pefI-srgC locus, which contains rck, was regulated by the temperature and SdiA, the transcriptional regulator ...
Lin Grace G Cellular & Molecular Biology Program, Univ Michigan, Ann Arbor, MI; Department of Molecular & Integrative Physiology, Univ Michigan, Ann Arbor, - - 2014
Expression of the Growth Hormone (GH)-stimulated gene Socs2 (Suppressor Of Cytokine Signaling 2) is mediated by the transcription activator STAT5 (Signal Transducer and Activator of Transcription 5) and the transcription repressor BCL6 (B-cell lymphoma 6). ChIP-Sequencing identified Cish (Cytokine-inducible SH2-containing protein) and Bcl6 as having similar patterns of reciprocal occupancy ...
El-Mowafy Somaia A SA Department of Microbiology, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt. Electronic address: - - 2014
Quorum sensing (QS) plays a vital role in regulation of virulence factors and toxins in Pseudomonas aeruginosa, which can cause serious human infections. Therefore, the QS system in P. aeruginosa may be an important target for pharmacological intervention. Activity of aspirin on the QS system was assessed using a reporter ...
Tran-Lundmark Karin K Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, - - 2014
Smooth muscle cell (SMC) proliferation is a key process in stabilization of atherosclerotic plaques, and during restenosis after interventions. A clearer understanding of SMC growth regulation is therefore needed to design specific anti-proliferative therapies. Retinoic acid has been shown to inhibit proliferation of SMCs both in vitro and in vivo ...
Farrokhnia Farkhondeh F University of Manchester, United - - 2014
Placental cell growth depends on an adaptable combination of an endogenous developmental programme and the exogenous influence of maternal growth factors, both of which may be influenced by microRNA (miR)-dependent effects on gene expression. We have previously shown that global miR suppression in placenta accelerates proliferation and enhances levels of ...
Hofmann Alejandro A National Children's Research Centre, Our Lady's Children's Hospital, Gate 5, Dublin, - - 2014
Caveolin-1 (Cav-1) exerts major regulatory functions on intracellular signaling pathways originating at the plasma membrane. Cav-1 is a key regulator in adverse lung remodeling and the development of pulmonary hypertension (PH) regulating vasomotor tone through its ability to reduce nitric oxide (NO) production. This low-output endothelial NO synthase (eNOS) derived ...
O'Hara Andrew - - 2014
Nucleolar sequestration of RelA is an important mechanism for regulating NF-κB transcriptional activity. COMMD1(MURR1) facilitated ubiquitination acts as a critical nucleolar targeting signal for RelA, but how this ubiquitination is regulated, and how it differs from cytokine-mediated ubiquitination which causes proteasomal degradation of RelA, is poorly understood. Here we report ...
Jackson Angelyca A AA Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, - - 2014
Hemolytic phospholipase C (PlcH) is a potent virulence and colonization factor that is highly expressed by Pseudomonas aeruginosa within the mammalian host. The phosphorylcholine liberated from phosphatidylcholine and sphingomyelin by PlcH is further catabolized into molecules that both support growth and further induce plcH expression. We have shown previously that ...
Almeida Luis Eduardo LE Department of Oral Sciences, Oral Surgery, Marquette University, School of Dentistry, Milwaukee, WI, - - 2014
Matrix metalloproteases (MMPs) are tissue-remodeling enzymes that function during the remodeling process, such as in immune-inflammatory diseases. Metalloprotease-2 (MMP-2) and metalloprotease-9 (MMP-9) are gelatinases that degrade several types of extracellular matrix collagen. It is hypothesized that in temporomandibular joint (TMJ) dysfunction, MMP-2 and MMP-9 expression levels may be elevated. Therefore, ...
Edwards Adrianne N AN Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, - - 2014
The anaerobic gastrointestinal pathogen Clostridium difficile must form a metabolically dormant spore to survive in oxygenic environments and be transmitted from host to host. The regulatory factors by which C. difficile initiates and controls the early stages of sporulation in C. difficile are not highly conserved with other Clostridium or ...
Chen Jianping J Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, - - 2014
The fruit of Ziziphus jujuba Mill., known as jujube or Chinese date, is commonly consumed as a health supplement worldwide. To study the role of jujube in brain benefits, the expression of neurotrophic factors and anti-oxidant enzymes in the jujube-treated cultured astrocytes was determined. Application of a chemical standardized water ...
Zhang Yingqian Y Department of Cardiology, Chinese PLA General Hospital, 28 Fuxing Rd, Beijing, 100853, - - 2014
Hypoxia/reoxygenation (H/R) is one of the cellular stresses in pathological conditions, such as myocardial infarction, stroke and organ transplantation. Oxidative stress caused by reactive oxygen species (ROS) is a crucial element of H/R injury in vascular endothelial cells (ECs). Xanthine oxidase (XO) has been recognized to contribute to H/R injury. ...
Yin Chaomin C Key Laboratory of Agro-Microbial Resources and Utilization, Ministry of Agriculture, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, - - 2014
The full-length cDNA of Pleurotus ostreatus superoxide dismutase (PoMn-SOD) was cloned and successfully expressed by using the pPIC9K vector under the control of alcohol oxidase 1 promoter with a secretion signal peptide (α-factor) in Pichia pastoris. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting demonstrated that recombinant PoMn-SOD, a ...
Guida Natascia N Division of Pharmacology, Department of Neuroscience, Reproductive and Odontostomatologic Sciences, School of Medicine, "Federico II" University of Naples, Via Pansini, 5, 80131 Naples, - - 2014
Phthalates, phthalic acid esters, are widely used as plasticizers to produce polymeric materials in industrial production of plastics and daily consumable products. Animal studies have shown that di(2-ethylhexyl)phthalate (DEHP) may cause toxic effects in rat brain. In the present study, chronic exposure to DEHP (0.1-100μM) caused dose-dependent cell death via ...
Boesmans Leen L Translational Research Center for Gastrointestinal Disorders (TARGID) and Leuven Food Science and Nutrition Research Centre (LFoRCe), University Hospital Gasthuisberg, KU Leuven, Herestraat 49 0&N1 box 701, 3000 Leuven, - - 2014
In ulcerative colitis (UC) the butyrate metabolism is impaired, leading to energy-deficiency in the colonic cells. The effect of inflammation on the butyrate metabolism was investigated. HT-29 cells were incubated with pro-inflammatory cytokines (TNF-α and/or IFN-γ) for 1h and 24h. Cells were additionally stimulated with butyrate to investigate its anti-inflammatory ...
Li Gang G Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, - - 2014
When Escherichia coli is grown in a medium lacking glucose or another preferred carbohydrate, the concentration of cyclic cAMP-cAMP receptor protein (cAMP-CRP) increases, and this latter complex regulates the expression of more than 180 genes. To respond rapidly to changes in carbohydrate availability, E. coli must maintain a suitable intracellular ...
Jeong Kwon K Department of Biochemistry and Molecular Biology (BK21 project), Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, #1, Hoegi-dong, Dongdaemoon-gu, Seoul, 130-701, Republic of - - 2014
Apelin, which is an endogenous ligand for the orphan G-protein-coupled receptor APJ, was reported to be up-regulated by hypoxia-inducible factor 1-α (HIF1-α) in hypoxia- and insulin-treated cell systems. However, a negative transcriptional regulator of apelin has not yet been identified. In this study, we showed that apelin is down-regulated by ...
Markell Lauren K LK DuPont Haskell Global Centers for Health and Environmental Sciences , Newark, Delaware 19714, United - - 2014
Xenobiotics may activate the estrogen receptor, resulting in alteration of normal endocrine functions in animals and humans. Consequently, this necessitates development of assay end points capable of identifying estrogenic xenobiotics. In the present study, we screened the potential estrogenicity of chemicals via their ability to induce vitellogenin (VTG) expression in ...
Wang C - - 2014
In addition to being positively regulated by prandial activity, bile acid production is also negatively controlled by the endocrine fibroblast growth factor 19 (FGF19) or the mouse ortholog FGF15 from the ileum that represses hepatic cholesterol 7 α-hydroxylase (Cyp7a1) expression through activating FGF receptor four (FGFR4). However, how these two ...
Zhang Junpeng J Faculty of Engineering, Dali University, Dali, CN. School of Information Technology and Mathematical Sciences, University of South Australia, AU. Children's Cancer Institute Australia, Randwick NSW 2301, AU. Kunming University of Science and Technology, Kunming, CN. Centre for Cancer Biology, SA Pathology, SA 5000, - - 2014
microRNAs (miRNAs) play crucial roles in complex cellular networks by binding to the messenger RNAs (mRNAs) of protein coding genes. It has been found that miRNA regulation is often condition-specific. A number of computational approaches have been developed to identify miRNA activity specific to a condition of interest using gene ...
Tudek Agnieszka A Centre de Génétique Moléculaire, CNRS UPR3404, 91190 Gif sur Yvette, - - 2014
The Nrd1-Nab3-Sen1 (NNS) complex is essential for controlling pervasive transcription and generating sn/snoRNAs in S. cerevisiae. The NNS complex terminates transcription of noncoding RNA genes and promotes exosome-dependent processing/degradation of the released transcripts. The Trf4-Air2-Mtr4 (TRAMP) complex polyadenylates NNS target RNAs and favors their degradation. NNS-dependent termination and degradation are coupled, ...
Nicholson Pamela P Department of Chemistry and Biochemistry, University of Berne, Berne, CH-3012, - - 2014
Eukaryotic mRNAs with premature translation-termination codons (PTCs) are recognized and eliminated by nonsense-mediated mRNA decay (NMD). NMD substrates can be degraded by different routes that all require phosphorylated UPF1 (P-UPF1) as a starting point. The endonuclease SMG6, which cleaves mRNA near the PTC, is one of the three known NMD ...
Ravasi Giulia G Department of Health Science, University Milano-Bicocca, Monza, - - 2014
Hepcidin transcription is strongly down-regulated under hypoxic conditions, however whether hypoxia inhibits hepcidin directly or indirectly is still unknown. We investigated the time course of hypoxia-mediated hepcidin down-regulation in vivo in healthy volunteers exposed to hypobaric hypoxia at high altitude and, based on the hypothesis that circulating factors are implicated ...
Shridhar Varsha - - 2014
The CD8 Antiviral Factor (CAF) suppresses viral transcription from the HIV-1 Long Terminal Repeat (LTR) promoter in a non-cytolytic manner. However, the region on the LTR upon which CAF acts is unknown. Our objective was to determine the region on the LTR upon which CAF acts to suppress HIV-1 transcription. ...
Wang Bing B State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan 430072, - - 2014
Mammalian viperin is a typical interferon (IFN)-induced antiviral protein. Fish have viperin homologs; however, little is known about the expression regulation of fish viperins. In this study, we report the expression regulation and antiviral function of a fish viperin from crucian carp Carassius auratus during IFN response. Crucian carp viperin ...
Takahashi Noriko N Laboratory of Physiological Chemistry, Institute of Medicinal Chemistry, Hoshi University, 2-4-41 Ebara, Shinagawa, Tokyo 142-8501, Japan. Electronic address: - - 2014
Melanin protects the skin against ultraviolet (UV) rays. It is produced in excess by UV radiation, which causes skin disorders and pigmentation. Retinoic acid (RA) decreases the levels of epidermal melanin by suppressing the expression of melanogenic enzymes including tyrosinase, which is the rate-limiting enzyme in melanin synthesis. However, RA ...
Yamamoto Kaneyoshi K Department of Frontier Bioscience, Hosei University, - - 2014
Expression pattern of the genome in Escherichia coli is controlled by regulating the utilization of a limited number of RNA polymerase between a total of 4,600 genes on its genome. The distribution pattern of RNA polymerase on the genome changes after two steps of protein-protein interaction with 7 sigma subunits ...
Liu Xuewei X R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China; Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of - - 2014
There has been little research reported on the synergism of plant expansins in cellulose hydrolysis because of the difficulty of heterologous expression. In this study, the expansin gene LeExp2 of Lycopersicum esculentum was expressed for the first time in Pichia pastoris to identify its function in vitro. The optimal expression ...
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