Search Results
Results 1 - 50 of 3420
1 2 3 4 5 6 7 8 9 10 >
Zhao Lianggong L The Second Clinical Medical College of Lanzhou University, 82 Cuiying Men, Lanzhou 730030, PR China; Orthopaedics Key Laboratory of Gansu Province, Lanzhou 730000, PR - - 2014
Radix Hedysari polysaccharides (HPS) is the principal active fraction of Radix Hedysari (RH). The information about HPS3d, the main fraction of HPS3, and its effect on bone is still unknown. In the present study, the purified HPS3d was obtained by anion-exchange column. It consisted of 94.38% polysaccharide, 3.40% protein and ...
Lei San-Lin SL Department of General Surgery, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. - - 2014
MicroRNAs (miRs) function as key regulators of gene expression and their deregulation is associated with the carcinogenesis of various cancers. In the present study, the aim was to validate the potential roles and regulatory mechanisms of miR-708 and miR-31 in colorectal cancer (CRC) cells. miR-708 and miR-31 were found to ...
Van Lint Sandra S Laboratory of Molecular and Cellular Therapy, Department of Biomedical Sciences, Medical School of the Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, B-1090 Jette, - - 2014
About 25 years ago, mRNA became a tool of interest in anticancer vaccination approaches. However, due to its rapid degradation in situ, direct application of mRNA was confronted with considerable skepticism during its early use. Consequently, mRNA was for a long time mainly used for the ex vivo transfection of ...
Gray G Kenneth GK Department of Cell, Developmental and Integrative Biology, 1900 University Blvd, THT 926A, University of Alabama at Birmingham, Birmingham, AL, 35294-0006, - - 2014
Since we last addressed the roles of NF-κB and JAK/STAT3 signaling in glioblastoma (GBM) 5 years ago, tremendous strides have been made in the understanding of these two pathways in glioma biology. Contributing to prosurvival mechanisms, cancer stem cell maintenance and treatment resistance, both NF-κB and STAT3 have been characterized ...
Goyama S S Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH, - - 2014
The transcription factor RUNX1 is a master regulator of hematopoiesis. Disruption of RUNX1 activity has been implicated in the development of hematopoietic neoplasms. Recent studies also highlight the importance of RUNX1 in solid tumors both as a tumor promoter and a suppressor. Given its central role in cancer development, RUNX1 ...
Liao Guoning G Center for Cell Biology and Cancer Research, Albany Medical College, Albany, NY, - - 2014
Cell migration plays an important role in many normal and pathological functions such as development, wound healing, immune defense, and tumor metastasis. Polarized migrating cells exhibit asymmetric distribution of many cytoskeletal proteins, which is believed to be critical for establishing and maintaining cell polarity and directional cell migration. To target ...
Weaver Ian C G IC Department of Psychology and Neuroscience, Dalhousie University, Life Science Research Institute, 1348 Summer Street, Halifax, Nova Scotia, Canada B3H - - 2014
Variations in maternal care in the rat influence the epigenetic state and transcriptional activity of glucocorticoid receptor (GR) gene in the hippocampus. The mechanisms underlying this maternal effect remained to be defined, including the nature of the relevant maternally regulated intracellular signalling pathways. We show here that increased maternal licking/grooming ...
Liu Jing-Jing JJ Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, P.R. - - 2014
MicroRNA-101 (miR-101) is frequently downregulated in various cancers. To date, the regulatory networks of miR-101 remain obscure. In this study, we demonstrated that miR-101 was mainly transcribed from human miR-101-2 and mouse miR-101bgene loci. Subsequent analyses revealed that activator protein-1 (AP-1) directly binded to the -17.4 to -16.4 k region upstream ...
Guiro J J Institute of Biosciences, University of Sao Paulo, Sao Paulo, - - 2014
The 164 bp U1 small nuclear (sn) RNA is one of the most abundant noncoding (nc) RNA in human cells, estimated to be in the region of 10(6) copies/cell. Although best known for its role in pre-messenger RNA (mRNA) splicing events, research over the past 20 years has revealed diverse ...
Batsché Eric E Institut Pasteur, Dpt Biologie du Développement et Cellules Souches, Unité de Régulation Epigénétique, 75015 Paris, France; URA2578, - - 2014
Alternative splicing of precursor RNAs is an important process in multicellular species because it impacts several aspects of gene expression: from the increase of protein repertoire to the level of expression. A large body of evidences demonstrates that factors regulating chromatin and transcription impact the outcomes of alternative splicing. Argonaute ...
Eichhorn Stephen W SW Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, - - 2014
MicroRNAs (miRNAs) regulate target mRNAs through a combination of translational repression and mRNA destabilization, with mRNA destabilization dominating at steady state in the few contexts examined globally. Here, we extend the global steady-state measurements to additional mammalian contexts and find that regardless of the miRNA, cell type, growth condition, or ...
Georg Jens J University of Freiburg, Faculty of Biology, D-79104 Freiburg, - - 2014
Little is known so far about RNA regulators of photosynthesis in plants, algae, or cyanobacteria. The small RNA PsrR1 (formerly SyR1) has been discovered in Synechocystis sp PCC 6803 and appears to be widely conserved within the cyanobacterial phylum. Expression of PsrR1 is induced shortly after a shift from moderate ...
Singh Noopur N Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow 226015, UP, India. Electronic address: - - 2014
microRNA is known to play an important role in growth and development of the plants and also in environmental stress. Ocimum basilicum (Basil) is a well known herb for its medicinal properties. In this study, we used in-silico approaches to identify miRNAs and their targets regulating different functions in O. ...
Trujillo Joyce J Facultad de Química, Department of Biology, UNAM, Ciudad Universitaria, México, D.F., - - 2014
Curcumin, a phenolic compound extracted from Curcuma longa, is commonly used in Asia as a spice and pigment and has several biological functions, particularly antioxidant properties. It has been reported that curcumin exhibits bifunctional antioxidant properties related to its capability to react directly with reactive oxygen species (ROS) and also ...
Han Leng - - 2014
Recent advances in RNA library preparation methods, platform accessibility and cost efficiency have allowed high-throughput RNA sequencing (RNAseq) to replace conventional hybridization microarray platforms as the method of choice for mRNA profiling and transcriptome analyses. RNAseq is a powerful technique to profile both long and short RNA expression, and the ...
Ghosh Anupama A Division of Plant Biology, Centenary campus, P1/12 C.I.T. Road Scheme VII M, Kolkata, 700054, West Bengal, - - 2014
Small heat shock proteins (HSP) play multiple functions within a cell. These functions primarily include regulation of growth and survival in response to different stresses. However in some cases small HSPs have been shown to play crucial roles in microbial pathogenesis. Ustilago maydis genome also codes for a number of ...
Montgomery Rusty L RL miRagen Therapeutics, Inc, Boulder, CO, - - 2014
Over the last decade, great enthusiasm has evolved for microRNA (miRNA) therapeutics. Part of the excitement stems from the fact that a miRNA often regulates numerous related mRNAs. As such, modulation of a single miRNA allows for parallel regulation of multiple genes involved in a particular disease. While many studies ...
Braicu C C Research Center for Functional Genomics and Translational Medicine, 'Iuliu Hatieganu' University of Medicine and Pharmacy, Cluj-Napoca, - - 2014
Exosomes are cell-derived vesicles that convey key elements with the potential to modulate intercellular communication. They are known to be secreted from all types of cells, and are crucial messengers that can regulate cellular processes by 'trafficking' molecules from cells of one tissue to another. The exosomal content has been ...
Sahin Ugur U 1] TRON Translational Oncology at the University Medical Center of the Johannes Gutenberg University, Langenbeckstrasse 1, 55131 Mainz, Germany. [2] BioNTech Corporation, An der Goldgrube 12, 55131 Mainz, - - 2014
In vitro transcribed (IVT) mRNA has recently come into focus as a potential new drug class to deliver genetic information. Such synthetic mRNA can be engineered to transiently express proteins by structurally resembling natural mRNA. Advances in addressing the inherent challenges of this drug class, particularly related to controlling the ...
Dong Jun J The Orthopedics Department, The Second Affiliated Hospital of Xi'an Jiaotong University, no. 157, West Five Road, Xi'an, 710004, - - 2014
Spinal cord injury (SCI) is medically and socioeconomically debilitating, and effective treatments are lacking. The elucidation of the pathophysiological mechanisms underlying SCI is essential for developing effective treatments for SCI. MicroRNAs (miRNAs) are small non-coding RNA molecules (18-24 nucleotides long) that regulate gene expression by interacting with specific target sequences. ...
Yu Ning N Department of Dermatology, Shanghai Skin Disease Hospital, Shanghai 200050, - - 2014
IL-1β is now emerging as a critical cytokine in the pathogenesis of Th17-mediated skin diseases, including psoriasis. Psoriatic keratinocytes are a major source of IL-1β, however, the mechanisms triggering IL-1β processing remain unknown. Recently, an acute-phase protein serum amyloid A (SAA) has been identified as a danger signal that triggers ...
Campos-Melo Danae D Molecular Medicine Group, Robarts Research Institute, Western University, London, ON, - - 2014
For many years, epidemiological studies have suggested an association between cancer and neurodegenerative disorders-two disease processes that seemingly have little in common. Although these two disease processes share disruptions in a wide range of cellular pathways, including cell survival, cell death and the cell cycle, the end result is very ...
Yan Junjie J State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of - - 2014
miRNAs are involved in the pollen development during the CMS occurrence in rice. miRNAs are 20-24 nt endogenously expressed small RNAs that play key roles in the regulation of many growth and developmental processes in plants. The knowledge on cytoplasmic male sterility (CMS) regulation by miRNAs in rice is rather ...
Wu Changgong C Rutgers University, United - - 2014
The dysregulation of protein oxidative post-translational modifications (PTMs) has been implicated in stress-related diseases. Trx1 is a key reductase that reduces specific disulfide bonds and other cysteine PTMs. Although commonly in the cytoplasm, Trx1 can also modulate transcription in the nucleus. However, few Trx1 nuclear targets have been identified due ...
Monroig-Bosque Paloma Del C PD Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA; University of Puerto Rico School of Medicine, San Juan 00936, Puerto - - 2014
One of the most fascinating discoveries in molecular oncology has been that cancer represents a disease in which genetic alterations in protein-coding, but also in non-coding genes complement each other. MicroRNAs (miRNAs) are a type of non-coding RNA (ncRNA) transcripts that can regulate gene expression primarily by disrupting messenger RNA ...
Gaber Rania - - 2014
BackgroundEpidermal Growth Factor Receptor (EGFR) targeting therapies are currently of great relevance for the treatment of lung cancer. For this reason, in addition to mutational analysis immunohistochemistry (IHC) of EGFR in lung cancer has been discussed for the decision making of according therapeutic strategies. The aim of this study was ...
Kim Won-Chan WC Department of Horticulture and Department of Forestry, Michigan State University, East Lansing, MI 48824-1222, USA; DOE-Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI - - 2014
AtC3H14 (At1g66810) is a plant-specific tandem CCCH zinc finger (TZF) protein that belongs to the 68-member CCCH family in Arabidopsis thaliana. In animals, TZFs have been shown to bind and recruit target mRNAs to the cytoplasmic foci where mRNA decay enzymes are active. However, it is not known whether plant ...
Meyuhas Oded O Department of Biochemistry and Molecular Biology, Institute for Medical Research - Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel. Electronic address: - - 2014
Cells encountering hostile growth conditions, like those residing in the middle of a newly developing solid tumor, conserve resources and energy by downregulating protein synthesis. One mechanism in this response is the translational repression of multiple mRNAs that encode components of the translational apparatus. This coordinated translational control is carried ...
Ma Yini Y Department of Combination of Chinese and Western Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, PR - - 2014
Peroxiredoxin 5 is a member of the peroxiredoxin family, which has been shown to act as an antioxidant whose main function is to reduce reactive oxygen species in cells. Peroxiredoxin 5 has been found to be abnormally elevated in human osteoarthritic chondrocytes. However, the detailed mechanism by which Peroxiredoxin 5 ...
Bloom Ryan J RJ Department of Bioengineering, Stanford University, Stanford, California, - - 2014
Synthetic genetic circuits incorporating regulatory components based on RNA interference (RNAi) have been used in a variety of systems. A comprehensive understanding of the parameters that determine the relationship between microRNA (miRNA) and target expression levels is lacking. We describe a quantitative framework supporting the forward engineering of gene circuits ...
Pascault Noémie N iEES Paris, UMR 7618 UPMC-CNRS-INRA-IRD-Paris 7-UPEC, 4 place Jussieu, 75005, Paris, - - 2014
Metatranscriptome analysis relates to the transcriptome of microbial communities directly sampled in the environment. Accessing the mRNA pool in natural bacterial communities presents some technical challenges such as the RNA extraction, rRNA depletion, and the choice of the high-throughput sequencing technique. The lack of technical details in scientific articles is ...
Ung Timothy H TH Department of Neurosurgery, University of Colorado School of Medicine, Aurora, CO, - - 2014
Exosomes are virus-sized, membrane enclosed vesicles with origins in the cellular endosomal system, but are released extracellularly. As a population, these tiny vesicles carry relatively enormous amounts of information in their protein, lipid, and nucleic acid content, and the vesicles can have profound impacts on recipient cells. This review employs ...
Zhang Lianglu L Pathogenic Organism and Infectious Diseases Research Institute, School of Basic Medical Sciences, Wuhan University, No. 185, Donghu Road, Wuchang District, Wuhan, 430071, - - 2014
Enterovirus 71 (EV71), a major causative agent of hand, foot, and mouth disease, has broken out several times and was accompanied by neurological disease. microRNAs, a class of small non-coding RNAs that are approximately 20 nucleotides long, play important roles in the regulation of various biological processes, including antiviral defense. ...
Gentry Leanna R LR University of North Carolina at Chapel Hill, Department of Pharmacology, Chapel Hill, NC, - - 2014
Since their discovery in 1986, Ral (Ras-like) GTPases have emerged as critical regulators of diverse cellular functions. Ral-selective guanine nucleotide exchange factors (RalGEFs) function as downstream effectors of the Ras oncoprotein, and the RalGEF-Ral signaling network comprises the third best characterized effector of Ras-dependent human oncogenesis. Because of this, Ral ...
Cushing Leah L Boston University School of Medicine, Boston, Massachusetts, United States ; - - 2014
Decades of studies have shown evolutionarily conserved molecular networks consisting of transcriptional factors, diffusing growth factors, and signaling pathways that regulate proper lung development. Recently, miRNAs, small non-coding regulatory RNAs, have been integrated into these networks. Significant advances have been made in characterizing the developmental stage- or cell type-specific miRNAs ...
Xie Meng M Center for Plant Science Innovation and School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68588-0660, - - 2014
microRNAs (miRNAs) are important regulators of gene expression. After excised from primary miRNA transcript by dicer-like1 (DCL1, an RNAse III enzyme), miRNAs bind and guide their effector protein named argonaute 1 (AGO1) to silence the expression of target RNAs containing their complementary sequences in plants. miRNA levels and activities are ...
Iwasaki Yasuno Y Department of Biochemistry and Cell Biology, Center for Developmental Genetics, Stony Brook University, Stony Brook, NY 11794-5215, - - 2014
Alternative splicing of pre-mRNAs is an important means of regulating developmental processes, yet the molecular mechanisms governing alternative splicing in embryonic contexts are just beginning to emerge. Polyglutamine-binding protein 1 (PQBP1) is an RNA-splicing factor that, when mutated, in humans causes Renpenning syndrome, an X-linked intellectual disability disease characterized by ...
He Fan F F He, Orthopaedic Institute, Soochow University, Suzhou, 215007, China - - 2014
Both self-renewal and lineage-specific differentiation of mesenchymal stem cells are triggered by their in vivo microenvironment including the extracellular matrix (ECM) and secreted hormones. The ECM may modulate the physiological functions of hormones by providing binding sites and by regulating downstream signaling pathways. Thus, the purpose of this investigation was ...
Zhou Xiuping X Brain Hospital, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, China; Insititute of Nervous System Diseases, Xuzhou Medical College, Xuzhou, Jiangsu, - - 2014
Protein kinase D2 (PKD2) has been demonstrated to promote tumorigenesis in many types of cancers. However, how PKD2 regulates cancer cell growth is largely unknown. In this study, we found that over-expression of PKD2 promoted glioma cell growth but down-regulation of PKD2 inhibited it. Further investigation indicated that PKD2 down-regulation ...
Jinks-Robertson Sue S Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710; email: - - 2014
Transcription requires unwinding complementaryDNAstrands, generating torsional stress, and sensitizing the exposed single strands to chemical reactions and endogenous damaging agents. In addition, transcription can occur concomitantly with the other major DNA metabolic processes (replication, repair, and recombination), creating opportunities for either cooperation or conflict. Genetic modifications associated with transcription are ...
Lacroix Chrystelle C CNRS/UPS Toulouse, - - 2014
Obstructive nephropathy is a frequently encountered situation in newborns. In previous studies, the urinary peptidome has been analyzed for the identification of clinically useful biomarkers of obstructive nephropathy. However, the urinary proteome has not been explored yet and should allow additional insight into the pathophysiology of the disease. We have ...
Csibi Alfredo A Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, - - 2014
Growth-promoting signaling molecules, including the mammalian target of rapamycin complex 1 (mTORC1), drive the metabolic reprogramming of cancer cells required to support their biosynthetic needs for rapid growth and proliferation [1]. Glutamine is catabolyzed to α-ketoglutarate (αKG), a tricarboxylic acid (TCA) cycle intermediate, through two deamination reactions, the first requiring ...
Wilczynska A A MRC Toxicology Unit, University of Leicester, Leicester, - - 2014
Since their discovery 20 years ago, miRNAs have attracted much attention from all areas of biology. These short (∼22 nt) non-coding RNA molecules are highly conserved in evolution and are present in nearly all eukaryotes. They have critical roles in virtually every cellular process, particularly determination of cell fate in development ...
Curinha Ana A Gene Regulation Group of Institute for Molecular and Cellular Biology (IBMC); University of Porto; Porto, Portugal; Department of Biology; University of Aveiro; Aveiro, - - 2014
Polyadenylation is the RNA processing step that completes the maturation of nearly all eukaryotic mRNAs. It is a two-step nuclear process that involves an endonucleolytic cleavage of the pre-mRNA at the 3'-end and the polymerization of a polyadenosine (polyA) tail, which is fundamental for mRNA stability, nuclear export and efficient ...
Mitic Tijana T Bristol Heart Institute, School of Clinical Sciences, University of Bristol, England, - - 2014
Epigenetic mechanisms may regulate the expression of pro-angiogenic genes, thus affecting reparative angiogenesis in ischemic limbs. The enhancer of zest homolog-2 (EZH2) induces thtrimethylation of lysine 27 on histone H3 (H3K27me3), which represses gene transcription. We explored: 1) if EZH2 expression is regulated by hypoxia and ischemia; 2) the impact ...
Kumar Virender V Department of Pharmaceutical Sciences, University of Nebraska Medical Center (UNMC), 986025 Nebraska Medical Center, Omaha, Nebraska, 68198-6025, - - 2014
Liver fibrosis is a pathological condition originating from liver damage that leads to excess accumulation of extracellular matrix (ECM) proteins in the liver. Viral infection, chronic injury, local inflammatory responses and oxidative stress are the major factors contributing to the onset and progression of liver fibrosis. Multiple cell types and ...
Dayekh Khalil K Cancer Therapeutics, Ottawa Hospital Research - - 2014
Targeting the epidermal growth factor receptor (EGFR), with inhibitors such as erlotinib, represents a promising therapeutic option in advanced Head and Neck Squamous Cell Carcinomas (HNSCC). However, they lack significant efficacy as single agents. Recently, we identified the ability of statins to induce synergistic cytotoxicity in HNSCC cells through targeting ...
Ye Julia J The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Urology, University of California, San Francisco, San Francisco, CA 94143, - - 2014
Establishment, maintenance, and exit from pluripotency require precise coordination of a cell's molecular machinery. Substantial headway has been made in deciphering many aspects of this elaborate system, particularly with respect to epigenetics, transcription, and noncoding RNAs. Less attention has been paid to posttranscriptional regulatory processes such as alternative splicing, RNA ...
Jakob Sissi S Department of Neuroscience, School for Mental Health and Neuroscience (MHENS), Maastricht University, Maastricht, The - - 2014
Prenatal stress (PS) exposure is known to increase the risk of developing emotional disorders like major depression in later life. However, some individuals do not succumb to adversity following developmental stress exposure, a phenomenon referred to as resilience. To date, the molecular mechanisms explaining why some subjects are vulnerable and ...
Van Pham Phuc P Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University, Ho Chi Minh City, Viet Nam. Electronic address: - - 2014
Numerous studies have sought to identify diabetes mellitus treatment strategies with fewer side effects. Mesenchymal stem cell (MSC) therapy was previously considered as a promising therapy; however, it requires the cells to be trans-differentiated into cells of the pancreatic-endocrine lineage before transplantation. Previous studies have shown that PDX-1 expression can ...
1 2 3 4 5 6 7 8 9 10 >