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Yadav Poonam P a National Bureau of Animal Genetic Resources , Karnal , - - 2014
Lactation is an important physiological process in dairy animals. During lactation, up to 85% of the body glucose is directed toward the mammary glands for milk synthesis. Studies related to lactation physiology are generally carried out on mammary biopsies, which may adversely affect animal health. In the present study, milk ...
Bai Yue-Chen YC College of Life and Basic Sciences, Sichuan Agricultural University, Ya'an, 625014, Sichuan, People's Republic of - - 2014
Tartary buckwheat (Fagopyrum tataricum Gaertn.) contains high concentrations of flavonoids. The flavonoids are mainly represented by rutin, anthocyanins, and proanthocyanins in tartary buckwheat. R2R3-type MYB transcription factors (TFs) play key roles in the transcriptional regulation of the flavonoid biosynthetic pathway. In this study, two TF genes, FtMYB1 and FtMYB2, were ...
Zielke Ryszard A RA Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR 97331, - - 2014
Vibrio cholerae, an etiological agent of cholera, circulates between aquatic reservoirs and the human gastrointestinal tract. The type II secretion (T2S) system plays a pivotal role in both stages of the lifestyle by exporting multiple proteins including cholera toxin. Here, we studied kinetics of expression of genes encoding the T2S ...
van der Felden Julia J Department of Genetics, Philipps-Universität, D-35043 Marburg, - - 2014
In Saccharomyces cerevisiae and related yeast species, the TEA transcription factor Tec1 together with a second transcription factor, Ste12, controls development including cell adhesion and filament formation. Tec1-Ste12 complexes control target genes through Tec1 binding sites (TCSs) that can be further combined with Ste12 binding sites (PREs) for cooperative DNA ...
Zhang Zhi-Chun ZC Key Laboratory of Molecular Medicine, Ministry of Education; Department of Biochemistry and Molecular Biology; Fudan University Shanghai Medical College, Shanghai 200032, P.R. - - 2014
Previous studies show that CCAAT/enhancer-binding protein α (C/EBPα) plays a very important role during adipocyte terminal differentiation and that AP-2α (Activator Protein 2α) acts as a repressor to delay the expression of C/EBPα. However, the mechanisms by which AP-2α prevents the expression of C/EBPα are not fully understood. Here we ...
Lu Pei P Key Laboratory of Etiology and Biosafety for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, - - 2014
YsrH is a novel cis-encoded sRNA located on the opposite strand to fabH2, which is essential for fatty acid biosynthesis in bacteria. In this study, YsrH-mediated regulation of fabH2 expression was investigated in Yersinia pseudotuberculosis. Constitutive and inducible over-expression of YsrH decreased the mRNA level of fabH2, while expression of ...
Proshkin Sergey S Engelhardt Institute of Molecular Biology, Russian Academy of Science, Moscow 119991, Russia; State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow 117545, - - 2014
Riboswitches are RNA sensors of small metabolites and ions that regulate gene expression in response to environmental changes. In bacteria, the riboswitch sensor domain usually controls the formation of a strong RNA hairpin that either functions as a potent transcription terminator or sequesters a ribosome-binding site. A recent study demonstrated ...
Agarwal Parinita P 1 Discipline of Wasteland Research, CSIR-Central Salt and Marine Chemicals Research Institute , G. B. Marg, Gujarat, India - - 2014
The WRKY family of transcription factors (TFs) play an intricate role in regulating the stress signaling pathways by autoregulation or may be by cross regulation through interaction with other proteins. Although WRKY TFs are considered to be plant specific, however, their presence has been reported from unicellular algae, slime mould, ...
Su Ying-Fang YF School of Dentistry, Chung Shan Medical University; Department of Stomatology, Chung Shan Medical University Hospital; Institute of Medicine, Chung Shan Medical - - 2014
Obesity has become a major public health problem of global significance. Today, aspirin remains the most commonly used medication for the treatment of pyrexia, pain, inflammation and antiplatelet. The present study aims at evaluating the possible existence of a putative p53-dependent pathway underlying the aspirin-induced inhibition of adipogenesis. Cell migration ...
Liu Chun-Lin CL Department of Neurosurgery, China Medical University Hospital, Taichung City, - - 2014
A nerve growth factor-differentiated PC12 cell line was used to investigate the protective effects of s-methyl cysteine (SMC) at 1, 2, 4, and 8 μM under oxygen-glucose deprivation (OGD) conditions. OGD decreased the cell viability. However, SMC pre-treatments at 2, 4 and 8 μM improved the cell viability, decreased cleaved ...
Jung Hyun Min - - 2014
While microRNAs (miRNAs) are extensively studied in post-transcriptional regulation of gene expressions in many biological processes, cellular miRNA-mediated regulation of viral genes remains unclear. In particular, the interplay between human papillomavirus (HPV) genes and miRNAs and how these interactions contribute to HPV-associated cancers remain elusive. Transient transfection of miR-375-mimic was ...
Baez Antonino - - 2014
The SoxRS regulon of E. coli is activated in response to elevated dissolved oxygen concentration likely to protect the bacteria from possible oxygen damage. The soxS expression can be increased up to 16 fold, making it a possible candidate for recombinant protein expression. Compared with the existing induction approaches, oxygen ...
Adamson S S Department of Biological Sciences, The University of Southern Mississippi, Hattiesburg, MS, - - 2014
The Gulf-Coast tick, Amblyomma maculatum, possesses an elaborate set of selenoproteins, which prevent the deleterious effects from oxidative stress that would otherwise occur during feeding. In the current work, we examined the role of selenoprotein K (SelK) and selenoprotein M (SelM) in feeding A. maculatum by bioinformatics, transcriptional gene expression, RNA ...
Touat-Hamici Zahia Z CNRS, UPR3404, - - 2014
Selenocysteine is inserted into selenoproteins via the translational recoding of a UGA codon, normally used as a stop signal. This process depends on the nature of the SECIS element located in the 3'UTR of selenoprotein mRNAs, selenium bioavailability, and possibly exogenous stimuli. To further understand the function and regulation of ...
Takarada-Iemata Mika M Department of Neuroanatomy, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Ishikawa, 920-8640, Japan; Japan Science and Technology Agency, CREST, Kawaguchi, Saitama, 332-0012, - - 2014
N-myc downstream-regulated gene 2 (Ndrg2) is a differentiation- and stress-associated molecule predominantly expressed in astrocytes in the central nervous system. In this study, we examined the expression and the role of Ndrg2 after cortical stab injury. We observed that Ndrg2 expression was elevated in astrocytes surrounding the wounded area as ...
Yang Chin-Ying CY Department of Agronomy; National Chung Hsing University; Taichung, - - 2014
Reactive oxygen species (ROS) have been reported to trigger signaling pathways that interact with other signaling pathways mediated by nitric oxide, lipid messengers, and plant hormones. In a previous study, we demonstrated that ethylene was involved in hypoxia signaling to regulate the expression of downstream genes such as AtERF73/HRE1 and ...
Zhong Huan H State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi, 530021, - - 2014
Growth hormone (GH) and its receptors are critical regulators of somatic growth and metabolism. It has been shown in mammals that the methylation of cytosines within the GH promoter plays a key role in regulating transcripts expression. In the present study, the GH, GHR1 and GHR2 proximal promoters were identified ...
Xia Jixing J Institute of Plant Science and Resources; Okayama University; Kurashiki, - - 2014
Plant roots play an important role in uptake of water and nutrients, support of above-ground part and environmental sensing, but the molecular mechanisms underlying the root development are poorly understood in rice. We found that a gene (OsASL1) encoding argininosuccinate lyase is involved in normal root development of rice. OsASL1 ...
Guarino Estrella E Tinbergen Building, Dept of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, United Kingdom. Electronic address: - - 2014
Synthesis of deoxynucleoside triphosphates (dNTPs) is essential for both DNA replication and repair and a key step in this process is catalyzed by ribonucleotide reductases (RNRs), which reduce ribonucleotides (rNDPs) to their deoxy forms. Tight regulation of RNR is crucial for maintaining the correct levels of all four dNTPs, which ...
Nagpal Kamalpreet K Beth Israel Deaconess Medical center, United - - 2014
Protein phosphatase 2A (PP2A) is a highly conserved and ubiquitous serine/threonine phosphatase. We have previously shown that PP2A expression is increased in T cells of SLE patients and this increased expression and activity of PP2A plays a central role in the molecular pathogenesis of SLE. Although the control of PP2A ...
Shah Suharsh S University of Calgary, - - 2014
Glucocorticoids act on the glucocorticoid receptor (NR3C1) to repress inflammatory gene expression. This is central to their anti-inflammatory effectiveness and rational improvements in therapeutic index depend on an understanding of mechanism. Human pulmonary epithelial A549 cells were used to study the role of the mitogen-activated protein kinase (MAPK) phosphatase, dual-specificity ...
Go Young Sam YS Department of Bioenergy Science and Technology, Chonam National University, Gwangju 500-757, - - 2014
The aerial parts of plants are protected from desiccation and other stress by surface cuticular waxes. The total cuticular wax loads and the expression of wax biosynthetic genes are significantly down-regulated in Arabidopsis thaliana under dark conditions. We isolated Decrease Wax Biosynthesis (DEWAX), which encodes an AP2/ERF-type transcription factor that ...
Feng Xiaoli X College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, - - 2014
TANK-binding kinase 1 (TBK1), a kinase at the crossroads of multiple IFN-inducing signaling pathways, plays essential roles in both antiviral and antibacterial innate immunity in mammals. Here, TBK1 gene (10339 bp) was identified and characterized from grass carp Ctenopharyngodon idella (CiTBK1). The genomic sequence is shorter than other orthologs in ...
Kawashima Tadashi T Department of Chemistry and Biochemistry and the Molecular Biology Institute, UCLA, Los Angeles, California, United States of - - 2014
Saccharomyces cerevisiae has been used as a model system to investigate the mechanisms of pre-mRNA splicing but only a few examples of alternative splice site usage have been described in this organism. Using RNA-Seq analysis of nonsense-mediated mRNA decay (NMD) mutant strains, we show that many S. cerevisiae intron-containing genes ...
Chaudhary Natasha N The University of Queensland, Institute for Molecular Bioscience, Queensland, - - 2014
Several studies have suggested crosstalk between different clathrin-independent endocytic pathways. However, the molecular mechanisms and functional relevance of these interactions are unclear. Caveolins and cavins are crucial components of caveolae, specialized microdomains that also constitute an endocytic route. Here we show that specific caveolar proteins are independently acting negative regulators ...
Zhang Lixin L Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. - - 2014
Peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) is a key coordinator of gene programs in metabolism and energy homeostasis in mammals. The aim of this study was to determine whether PGC-1α is involved in the transcriptional regulation of retinal neovascularization in oxygen-induced retinopathy (OIR). The expression of PGC-1α in the retina of ...
Chong Phyllis Sy PS Cancer Science Institute of Singapore, National University of - - 2014
PRL-3, an oncogenic dual-specificity phosphatase, is over-expressed in 50% of AML and associated with poor survival. We found that stable expression of PRL-3 confers cytokine independence and growth advantage of AML cells. However, how PRL-3 mediates these functions in AML is not known. To comprehensively screen for PRL3-regulated proteins in ...
Duan Liu L National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, - - 2014
Blast, caused by the fungus Magnaporthe oryzae (M. oryzae), is one of the most devastating diseases of rice worldwide. Phenylalanine ammonia-lyase (PAL) is a key enzyme in the phenylpropanoid pathway, which leads to biosynthesis of the defense-related phytohormone salicylic acid (SA) and flavonoid-type phytoalexins sakuranetin and naringenin. However, the roles ...
Pagliai Fernando A FA Department of Microbiology and Cell Science, Genetics Institute, Institute of Food and Agricultural Sciences, University of Florida. 2033 Mowry road. PO Box 103610. Gainesville, FL 32610-3610, - - 2014
In this study we uncover two genes in Lactobacillus brevis ATCC367, tstT and tstR, encoding for a rhodanese and a transcriptional regulator involved in cyanide detoxification. TstT (LVIS_0852) belongs to a new class of thiosulfate:cyanide sulfurtransferases. We found that TstR (LVIS_0853) modulates both the expression and the activity of the ...
Cecere Germano G Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York, - - 2014
Argonaute proteins and their small RNA cofactors short interfering RNAs are known to inhibit gene expression at the transcriptional and post-transcriptional levels. In Caenorhabditis elegans, the Argonaute CSR-1 binds thousands of endogenous siRNAs (endo-siRNAs) that are antisense to germline transcripts. However, its role in gene expression regulation remains controversial. Here ...
Li Xiao-Lan XL College of Life Sciences, Liaoning Normal University; Key Laboratory of Plant Biotechnology in Liaoning Province, 1 South Liushu Street, Ganjingzi District, Dalian, 116081, Liaoning, China, - - 2014
Sl NAC1 functions as a stress-responsive NAC protein involved in the abscisic acid-dependent signaling pathway and enhances transgenic Arabidopsis drought, salt, and cold stress tolerance. NAC (NAM, ATAF1, 2, CUC2) transcription factors constitute the largest families of plant-specific transcription factors, known to be involved in various growth or developmental processes ...
Franasiak Jason M JM Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology and Reproductive Science, Robert Wood Johnson Medical School, Rutgers University, New Brunswick, New - - 2014
To evaluate endometrial leukemia inhibitor factor (LIF) expression as a marker of endometrial receptivity in women with unexplained infertility (UI). Prospective case-control study. University-associated infertility clinics. Women with UI for more than 1 year and healthy control women. Endometrial biopsy. Time to pregnancy was compared between patients with UI who were ...
Mahammad Azhar A a Jawaharlal Technological University, Biotechnology , Hyederabad , - - 2014
Enteropeptidase (E.C.3.4.21.9) is the glycoprotein enzyme in the small intestine that triggers the activation of the zymogens in pancreatic juice by converting trypsinogen into trypsin. Because of its physiological significance, there have been many studies on the expression, purification, and characterization of Enteropeptidase from different species. The baculovirus expression system ...
Chi-Wei Lan John J Biorefinery & Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan, Taiwan; The Agricultural Biotechnology Research Center (ABRC), Academia Sinica, Taipei, Taiwan. Electronic address: - - 2014
A mutant gene of rumen phytase (phyA-7) was cloned into pET23b(+) vector and expressed in the Escherichia coli BL21 under the control of the T7 promoter. The study of fermentation conditions includes the temperature impacts of mutant phytase expression, the effect of carbon supplements over induction stage, the inferences of ...
Choi Yun Jung YJ Division of Rheumatology, Department of Internal Medicine, Chonbuk National University Medical School and Research Institute of Clinical Medicine of Chonbuk National University Hospital-Chonbuk National University, San 2-20 Geumam-dong, Deokjin-gu, Jeonju, Jeonbuk, 561-180, South - - 2014
This study was performed to define the effects of sulforaphane on interleukin-1β (IL-1β)-induced proliferation of rheumatoid arthritis synovial fibroblasts (RASFs), the expression of matrix metalloproteinases (MMPs) and cyclooxygenase (COX), and the production of prostaglandin E2 (PGE2) by RASFs. The proliferation of RASFs was evaluated with CCK-8 reagent in the presence ...
Zhong Hua H Department of Urology and Winship Cancer Institute, Emory University School of Medicine, Atlanta, Georgia, 30322; Rutgers Cancer Institute of New Jersey and Department of Pathology and Laboratory Medicine, Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey, - - 2014
Hypoxia-inducible factor 1α (HIF-1α) is the regulatory subunit of the heterodimeric HIF-1 that plays a critical role in transcriptional regulation of genes in angiogenesis and hypoxic adaptation, while fatty acid metabolism mediated by lipoxygenases has been implicated in a variety of pathogeneses, including cancers. In this study, we report that ...
Zhang Xing X State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, - - 2014
The apical hook is an essential structure that enables epigeal plants to protrude through the soil. Arabidopsis thaliana HOOKLESS1 (HLS1) is reported to be a key regulator of hook development and a direct target gene of the ethylene (ET)-activated transcription factors ETHYLENE INSENSITIVE3 (EIN3) and its close homolog EIN3-Like1. Previous ...
Zhao L L Department of Animal and Poultry - - 2014
We evaluated the effect of GH on the differentiation of primary bovine preadipocytes into adipocytes. Bovine preadipocytes, derived from adipose tissue explants, were induced to differentiate into adipocytes in the presence or absence of recombinant bovine GH. The differentiation status of adipocytes was assessed by Oil Red O staining and ...
Ballard Shannon L SL Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI - - 2014
Toll-like receptors (TLRs) are best characterized for their roles in mediating dorsoventral patterning and the innate immune response. However, recent studies indicate that TLRs are also involved in regulating neuronal growth and development. Here, we demonstrate that the TLR Tollo positively regulates growth of the Drosophila melanogaster larval neuromuscular junction ...
Chakraborty Prarthana P Division of Cell Biology and Physiology; CSIR-Indian Institute of Chemical - - 2014
Three phenolic glycosides isolated from the heartwood of Pterocarpus marsupium showed significant free radical and superoxide ion scavenging activity and antioxidant potential that were comparable to, or several folds higher than those of standard antioxidants, trolox and ascorbic acid. The effective concentrations of these compounds were far below their cytotoxic ...
Oh Dong Hoon DH Department of Psychiatry, College of Medicine and Institute of Mental Health, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 133-791, Republic of - - 2014
Previous human postmortem studies have shown that expression of glutamate transporters (SLC1A2 and SLC1A3) and gamma-aminobutyric acid-synthesizing enzyme [glutamic acid decarboxylase 1 (GAD1)] are reduced in the dorsolateral prefrontal cortex (dlPFC) in subjects with major depressive disorder (MDD). However, no studies have explored the association between these two molecules and ...
Mai Weijun W The Institute of Life Science, Jiangsu University, Zhenjiang, 212013, China, - - 2014
The inhibitor κB (IκB) protein is an important component of the nuclear factor kB (NF-κB) signaling pathway. We have cloned and characterized the IκB-α homologue gene (named EaIκB-α) from grouper (Epinephelus akaara), expressed it in Escherichia coli and generated a rabbit polyclonal antibody. EaIκB-α encodes a 308-amino acid protein with ...
Craig Theodore A TA 1 Nephrology and Hypertension Research, Department of Internal Medicine, Mayo Clinic , Rochester, - - 2014
Abstract The sterol hormone, 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3), regulates gene expression and messenger RNA (mRNA) concentrations in zebrafish in vivo. Since mRNA concentrations and translation are influenced by micro-RNAs (miRNAs), we examined the influence of 1α,25(OH)2D3 on miRNA expression in zebrafish in vivo with whole transcriptome RNA sequencing, searched for miRNA ...
Gurunathan Sujatha S Columbia University, United - - 2014
Fibroblast growth factor-inducible 14 (Fn14) is a highly inducible cytokine receptor that engages multiple intracellular signaling pathways, including nuclear factor-kappaB (NF-κB) and mitogen-activated protein kinase (MAPK). Fn14 expression is regulated by several cytokines and growth factors, and Fn14 is transiently upregulated after injury. In contrast, in states of chronic inflammatory ...
Ray Sutapa S Sealy Center for Molecular Medicine, University of Texas Medical Branch, Galveston, TX 77555; Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX - - 2014
Signal Transducers and Activator of Transcription-3 (STAT3) are latent transcription factors that are regulated by post-translational modifications (PTMs) in response to cellular activation by the IL-6 superfamily of cytokines to regulate cell cycle progression and/or apoptosis. Here we observe that STAT3 is inducibly mono-ubiquitinated and investigate its consequences. Using domain ...
Anasontzis George E - - 2014
Fusarium oxysporum is among the few filamentous fungi that have been reported of being able to directly ferment biomass to ethanol in a consolidated bioprocess. Understanding its metabolic pathways and their limitations can provide some insights on the genetic modifications required to enhance its growth and subsequent fermentation capability. In ...
Kim Yong Hyun YH Department of Biology, Soonchunhyang University, Asan, Chungnam, 336-745, Republic of - - 2014
This study was conducted to evaluate the physiological effects of different selenium (Se) levels on the growth of white-rot fungus, Pleurotus eryngii, with special reference to the regulation of ligninolytic enzymes such as laccase and versatile peroxidase. The fungus was grown in medium supplemented with 1, 10, 100, 1,000 and ...
Guo Feng-Jin FJ Department of Cell Biology and Genetics, Core Facility of Development Biology, Chongqing Medical University, Chongqing, - - 2014
We previously reported that transcription factor XBP1S binds to RUNX2 and enhances chondrocyte hypertrophy through acting as a cofactor of RUNX2. Herein, we report that XBP1S is a key downstream molecule of BMP2 and is required for BMP2-mediated chondrocyte differentiation. XBP1S is up-regulated during chondrocyte differentiation and demonstrates the temporal ...
Kim Young Ho YH Research Institute for Agriculture and Life Sciences, Seoul National University, 599 Gwanakno Gwanakgu, Seoul 151-742, Republic of - - 2014
Various molecular forms of acetylcholinesterase (AChE) have been characterized in insects. Post-translational modification is known to be a major mechanism for the molecular diversity of insect AChE. However, multiple forms of Drosophila melanogaster AChE (DmAChE) were recently suggested to be generated via alternative splicing (Kim and Lee, 2013). To confirm ...
Fu Xiaodong X Department of Physiology, School of Basic Science, Guangzhou Medical University, Guangzhou, Guangdong Province, - - 2014
Oxysterol is associated with the induction of endothelial oxidative stress and impaired endothelial function. Mitochondria play a central role in oxidative energy metabolism and the maintenance of proper redox status. The purpose of this study was to determine the effects and mechanisms of 7-ketocholesterol (7-KC) on isocitrate dehydrogenase 2 (IDH2) ...
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