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Cui Qing-Ke QK Department of Neurosurgery, The Qilu Hospital of Shandong University, Jinan, Shandong, 250012, P. R. China; Liaocheng People's Hospital, Liaocheng, Shandong, 252000, P. R. - - 2014
To investigate the effect of microRNA (miR-184) on regulating the genesis, development and proliferation of glioma cells. Lipidosome was used to transfect miR-184 mimic and inhibitor to glioma cell line, and the cell proliferation ability changes were determined by MTT and plate cloning experiment after the transfection. WB test was ...
Prochiantz Alain A CIRB, CNRS UMR 7241/INSERM U1050, College de France, 11 place Marcelin Berthelot, Paris Cedex 05 75231, France - - 2014
Homeoprotein (HP) transcription factors were originally identified for their embryonic cell-autonomous developmental functions. In this review, we discuss their postnatal and adult physiological functions based on the study of Otx2, Engrailed-1 and Engrailed-2 (collectively Engrailed). For Engrailed, we discuss its function in the cell-autonomous regulation of ventral midbrain dopaminergic neuron ...
Barmack Neal H NH Department of Physiology and Pharmacology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA - - 2014
Synaptic activation of central neurons is often evoked by electrical stimulation leading to post-tetanic potentiation, long-term potentiation or long-term depression. Even a brief electrical tetanus can induce changes in as many as 100 proteins. Since climbing fibre activity is often associated with cerebellar behavioural plasticity, we used horizontal optokinetic stimulation ...
Guo Zhentao Z Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, - - 2014
Leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) are an inhibitor of receptor tyrosine kinases (RTKs) that was discovered in recent years, and many studies showed that LRIG1 is a tumor suppressor gene and may be related to tumor drug resistance. In this study, we explored whether LRIG1 protein expression can ...
Sung Hye Youn HY Department of Biochemistry, School of Medicine, Ewha Womans University, Seoul, - - 2014
Recent discoveries suggest that aberrant DNA methylation provides cancer cells with advanced metastatic properties. However, the precise regulatory mechanisms controlling metastasis genes and their role in metastatic transformation are largely unknown. To address epigenetically-regulated gene products involved in ovarian cancer metastasis, we examined the mechanisms regulating mucin 13 (MUC13) expression ...
Dwyer Joseph J Randall Division of Cell and Molecular Biophysics and Cardiovascular Division, British Heart Foundation Centre of Research Excellence, King's College London, SE1 1UL, United - - 2014
Members of the formin family are known to be involved in the regulation of the actin cytoskeleton. We have recently identified a muscle specific splice variant of the formin FHOD3 and demonstrated its role in the maintenance of the contractile filaments of cardiomyocytes. Here, we characterize the expression and subcellular ...
Tripathi Subhash K SK Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland; National Doctoral Programme in Informational and Structural Biology, Turku, Finland; Turku Doctoral Programme of Molecular Medicine (TuDMM), University of Turku, Turku, - - 2014
Combined with TCR stimuli, extracellular cytokine signals initiate the differentiation of naive CD4(+) T cells into specialized effector T-helper (Th) and regulatory T (Treg) cell subsets. The lineage specification and commitment process occurs through the combinatorial action of multiple transcription factors (TFs) and epigenetic mechanisms that drive lineage-specific gene expression ...
Di Santo James P JP Innate Immunity Unit, Institut Pasteur, Paris, France; Inserm U668, Institut Pasteur, Paris, - - 2014
Innate and adaptive lymphocytes are characterized by phenotypic and functional characteristics that result from genomic rearrangements (in the case of antigen-specific B and T cells) coupled with selective gene expression patterns that are generated in a context-dependent fashion. Cell-intrinsic expression of transcription factors (TFs) play a critical role in the ...
Imanaka-Yoshida Kyoko K Department of Pathology and Matrix Biology, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan; Mie University Research Center for Matrix Biology, Tsu, Mie, 514-8507, - - 2014
Tenascin-C (TNC) is an extracellular glycoprotein categorized as a matricellular protein. It is highly expressed during embryonic development, wound healing, inflammation, and cancer invasion, and has a wide range of effects on cell response in tissue morphogenesis and remodeling including the cardiovascular system. In the heart, TNC is sparsely detected ...
Shan Ren Feng RF Department of General Surgery, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. - - 2014
In the present study, the effect of Aurora-B inhibition on HepG2 cell invasion and migration in vitro was investigated. A recombinant plasmid targeting the Aurora-B gene (MiR-Aurora-B) was used to inhibit Aurora-B expression in HepG2 cells. Cell migration and invasion were investigated using Transwell migration and invasion assays. The results ...
Wu Shey-Lin SL Department of Neurology, Chang-Hua Christian Hospital, Changhua, Taiwan, Republic of - - 2014
Homeobox genes encode transcription factors that regulate embryonic development programs including organogenesis, axis formation and limb development. Previously, we identified and cloned a mouse double homeobox gene, Duxbl, whose homeodomain exhibits the highest identity (67 %) to human DUX4, a candidate gene of facioscapulohumeral muscular dystrophy (FSHD). Duxbl proteins have been ...
Huang Kuo-Hsiung - - 2014
BackgroundOur previous study showed NF-¿B repressing factor (NKRF) downregulates IP-10 and IL-8 synthesis in the peripheral blood mononuclear cells and alveolar macrophages of TB patients with high bacterial loads. However, the mechanism underlying the repressive effect of NKRF is not fully understood.ResultsThe levels of IP-10, IL-8 and NKRF were significantly ...
Zhao Guangfeng - - 2014
BackgroundMesenchymal stem cells (MSCs) at maternal-fetal interface are considered to play an important role in the pathogenesis of pre-eclampsia (PE). microRNAs (miRNAs) also have an important influence on differentiation, maturation, and functions of MSCs. Our aim in this study was to determine the differential expression of miRNAs in decidua-derived MSCs ...
Lu Bin B College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei, People's Republic of - - 2014
Altered gene expression coincides with leukemia development and may affect distinct features of leukemic cells. PITHD1 was significantly downregulated in leukemia and upregulated upon PMA induction in K562 cells undergoing megakaryocyte differentiation. We aimed to study the function of PITHD1 in megakaryocyte differentiation. K562 cells and fetal liver cells were ...
Diaz-Rodriguez Esther E CIMUS (E.D.-R., A.R.G.-G., M.G.-L., C.D., C.V.A.), University of Santiago de Compostela, and Department of Endocrinology (I.B.), University Hospital (CHUS), IDIS, Santiago de Compostela, Spain; Departments of Cell Biology, Physiology, and Immunology (A.I.-C., E.G.-P., R.M.L., J.P.C.), and Morphological Sciences (L.J.-R.), University of Cordoba, and Reina Sofia University Hospital (P.B., M.A.G.), Maimonides Institute for Research in Biomedicine of Cordoba, Córdoba, Spain; Departments of Endocrinology and Pathology (A.L., M.A.J., A.S., E.V.), Hospital Universitario Virgen del Rocío, IBIS-CSIC, University of Sevilla, Sevilla, Spain; Department of Endocrinology (F.J.T.), Hospital Virgen de la Victoria, and Department of Endocrinology (J.A.G.-A.), Hospital Carlos Haya, Malaga, Spain; and CIBERObn Network (A.I.-C., A.L., F.J.T., P.B., L.J.-R., I.B., C.D., R.M.L., J.P.C., - - 2014
Acromegaly is caused by somatotroph cell adenomas (somatotropinomas [ACROs]), which secrete GH. Human and rodent somatotroph cells express the RET receptor. In rodents, when normal somatotrophs are deprived of the RET ligand, GDNF, RET is processed intracellularly to induce overexpression of Pit1, which in turn leads to p19Arf/p53-dependent apoptosis. Our ...
Lee Su Ui SU Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk 363-883, - - 2014
The beta2 adrenergic receptor (ADRB2) is a G protein-coupled transmembrane receptor expressed in the human respiratory tract and widely recognized as a pharmacological target for treatments of asthma and chronic obstructive pulmonary disorder (COPD). Although a number of ADRB2 agonists have been developed for use in asthma therapy, indacaterol is ...
Lai Jing J Department of Medical Oncology, Jinling Hospital, School of Medicine, Southern Medical University, Guangzhou, 510282, - - 2014
The p73 gene contains an extrinsic P1 promoter and an intrinsic P2 promoter, controlling the transcription of the pro-apoptotic TAp73 isoform and the anti-apoptotic ΔΝp73 isoform, respectively. The DNA methylation status of both promoters act equally in the epigenetic transcriptional regulation of their relevant isoforms. The aim of this study ...
Bao Zhao-Shi ZS Beijing Neurosurgical - - 2014
Studies of gene rearrangements and the consequent oncogenic fusion proteins have laid the foundation for targeted cancer therapy. To identify oncogenic fusions associated with glioma progression, we catalogued fusion transcripts by RNA-seq of 272 WHO grade II, III, and IV gliomas. Fusion transcripts were more frequently found in high grade ...
Balestra Fernando R FR Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, - - 2014
During differentiation of multiciliated cells, numerous centrioles are generated in each cell to act as templates for the formation of a corresponding number of cilia. A new study reveals that multicilin, a protein required for multiciliogenesis, is a key component of a regulatory complex that activates the transcription of genes ...
Samuel Temesgen - - 2014
The nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB) signaling pathway is activated in cells exposed to various stimuli, including those originating on the cell surface or in the nucleus. Activated NF-kappaB signaling is thought to enhance cell survival in response to these stimuli, which include chemotherapy and radiation. In ...
Beilfuss Anja A Department of Gastroenterology and Hepatology, University Hospital, University Duisburg-Essen, Essen, - - 2014
Non-alcoholic fatty liver disease (NAFLD) is closely linked to obesity and constitutes part of the metabolic syndrome, which have been associated with low serum vitamin D (VD). Due to known crosstalk between VD and transforming growth factor (TGF)-β signalling, VD has been proposed as an antifibrotic treatment. We evaluated the ...
Watanabe Yukiko Y Departments of - - 2014
Epigenetic modifications, such as posttranslational modifications of histones, play an important role in gene expression and regulation. These modifications are in part mediated by the Trithorax group (TrxG) complex and the Polycomb group (PcG) complex, which activate and repress transcription, respectively. We herein investigate the role of Menin, a component ...
Agrawal Rahul - - 2014
Hypoxia is a critical aspect of the glioma microenvironment and has been associated with poor prognosis and resistance to various therapies. However, the mechanisms responsible for hypoxic survival of glioma cells remain unclear. Recent studies strongly suggest that microRNAs act as critical mediators of the hypoxic response. We thus hypothesized ...
Leite Fábio Renato Manzolli FR School of Dentistry, Federal University at Pelotas (UFPel), Pelotas, RS, - - 2014
The myeloid differentiation factor 88 (MyD88) plays a pivotal role in Toll-like receptor (TLR)- and interleukin-1 receptor (IL-1R)-induced osteoclastogenesis. We examined the role of MyD88 on p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) activation and nucleotide-binding oligomerization domain (Nod) induction by lipopolysaccharide (LPS) ...
Srivastava Jyoti J Department of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, - - 2014
Retinoid X receptor (RXR) regulates key cellular responses such as cell growth and development, and this regulation is frequently perturbed in various malignancies, including hepatocellular carcinoma (HCC). However, the molecule(s) that physically govern this deregulation are mostly unknown. Here, we identified RXR as an interacting partner of astrocyte-elevated gene-1 (AEG-1)/metadherin ...
Schönitzer Veronika V 1 Experimental Surgery and Regenerative Medicine, Department of Surgery, Ludwig-Maximilians-University , 80336, Munich, Germany - - 2014
Abstract Human mesenchymal stem cells (hMSCs) are a promising target for cell-based bone regeneration. However, their application for clinical use is limited because hMSCs lose their ability for cell division and differentiation during longer in vitro cultivation. The osteogenic differentiation is regulated through a complex network of molecular signal transduction ...
Penumatsa Krishna C KC Tufts Medical - - 2014
We previously reported that transglutaminase 2 (TG2) activity is markedly elevated in lungs of hypoxia-exposed rodent models of pulmonary hypertension (PH). Since vascular remodeling of pulmonary artery smooth muscle cells (PASMCs) is important in PH, we undertook the present study to determine if TG2 activity is altered in PASMCs with ...
Fang Yong - - 2014
p27Kip1 is a potent inhibitor of cyclin-dependent kinases that drive G1 to S cell-cycle transition and reduced p27Kip1 expression is prevalent in a wide range of human tumors, the exact mechanism(s) of p27Kip1-mediated tumor suppression remains obscure. In this study, we identified a close, inverse relationship between p27Kip1 and EGFR ...
Bohinc Brittany N BN Division of Endocrinology, Diabetes, and Metabolism, Duke University, Durham, - - 2014
Thyroid hormone (TH) is important for tissue repair because it regulates cellular differentiation. Intrahepatic TH activity is controlled by both serum TH levels and hepatic deiodinases. TH substrate (thyroxine, T4) is converted into active hormone (triidothyronine, T3) by deiodinase 1 (D1), but into inactive hormone (reverse T3, rT3) by deiodinase ...
Zhong Zhaopeng Z Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. - - 2014
MicroRNA (miR)‑30c has been identified as a tumor suppressor gene in numerous diseases. Aberrant miR‑30c expression has been associated with the invasion of different types of cancer. However, the potential mechanisms underlying the association between miR‑30c and invasion has been poorly elucidated in non‑small‑cell lung cancer (NSCLC). In the present ...
Song Zhenguo Z Department of Pancreatic Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, China, - - 2014
The aim of study was to discuss the correlation and regulatory mechanism of HIF-1 and miR-191 expression in pancreatic tumor. The association between the miR-191 and the clinicopathologic characteristics and the prognosis of pancreatic cancer was further explored. After hypoxic cultured for 6 and 12 h, qRT-PCR and Western blot were ...
Hu Dan-Ning DN Tissue Culture Center, New York Eye and Ear Infirmary, New York Medical College, New York, NY, 10003, United States - - 2014
Purpose: Melanocytes are one of the major cellular components in the uvea. IL-8/CXCL8 and monocyte chemoattractant protein-1 (MCP-1/CCL2) are the two most important pro-inflammatory chemokines. We studied the constitutive and lipopolysaccharide (LPS)-induced expression of IL-8 and MCP-1 in cultured human uveal melanocytes (UM), and explored the relevant signal pathways. Methods: ...
Dabir Snehal S Division of Hematology/Oncology, Case Western Reserve University/University Hospitals of - - 2014
Purpose: Deregulation of STAT3 activation is a hallmark of many cancer cells and the underlying mechanisms are subject to intense investigation. We examined the extent of PIAS3 expression in mesothelioma cells and human tumor samples and determined the functional effects of PIAS3 expression on STAT3 signaling. Experimental design: We evaluated ...
Ok Chi Y CY Hematopathology, The University of Texas MD Anderson Cancer - - 2014
Purpose: Activated signal transducer and activator of transcription 3 (STAT3) regulates tumor growth, invasion, cell proliferation, angiogenesis, immune response and survival. Data regarding expression of phosphorylated (activated) STAT3 in diffuse large B-cell lymphoma (DLBCL) and the impact of phosphorylated STAT3 (pSTAT3) on prognosis are limited. Experimental Design: We evaluated expression ...
Abdelmohsen Kotb K Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA - - 2014
Noncoding RNAs (ncRNAs) and RNA-binding proteins are potent post-transcriptional regulators of gene expression. The ncRNA 7SL is upregulated in cancer cells, but its impact upon the phenotype of cancer cells is unknown. Here, we present evidence that 7SL forms a partial hybrid with the 3'-untranslated region (UTR) of TP53 mRNA, ...
Kasim Mumtaz M Charite - Universitaetsmedizin Berlin, - - 2014
The basic helix-loop-helix transcription factor hASH1, encoded by the ASCL1 gene, plays an important role in neurogenesis and tumor development. Recent findings indicate that the local oxygen tension is a critical determinant for the progression of neuroblastomas. Here we investigated the molecular mechanisms underlying the oxygen-dependent expression of hASH1 in ...
Zhu Hongwu H Department of - - 2014
Multidrug resistance (MDR) is a major challenge to the clinical treatment of esophageal cancer. The stress response gene activating transcription factor 4 (ATF4) is involved in homeostasis and cellular protection. However, relatively little is known about the expression and function of ATF4 in esophageal squamous cell carcinoma (ESCC) MDR. In ...
Li Jianwei J Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, - - 2014
The aberrant expression of PSMD10 has important functions in various malignancies. This study showed that PSMD10 was highly expressed and inversely correlated with the expression of miR-605 in intrahepatic cholangiocarcinoma (ICC) specimens. MiR-605 directly targeted and repressed PSMD10 expression. In addition, over-expression of miR-605 inhibited ICC cell progression both in ...
Uruno Akira A Department of Medical Biochemistry, Tohoku University Graduate School of Medicine, Sendai 980-8575, - - 2014
Cushing's disease is a disorder caused by excessive ACTH secretion from a corticotroph tumor of the pituitary gland. Although its standard therapy is a transsphenoidal surgery, innovation of novel medical treatments for the disease is urgently necessary. Retinoic acid (RA) has been reported to suppress adrenocorticotropic hormone (ACTH) secretion in ...
Rowe Martin M School of Cancer Sciences, The University of Birmingham, Vincent Drive, Birmingham, - - 2014
A number of diverse environmental cues have been linked to B lymphocyte differentiation and activation. One such cue, Notch-2, may be particularly relevant to the biology of Epstein Barr virus (EBV) infection which colonizes the B cell compartment. Activated Notch and EBV nuclear antigen, EBNA2, both function as transcriptional activators ...
Park Seong-Hwan SH Pusan National University School of Medicine, Korea, Republic - - 2014
The cell-protective features of the ER stress response are chronically activated in vigorously growing malignant tumor cells, which provide cellular growth advantages over the adverse microenvironment including chemotherapy. As an intervention with ER stress responses in the intestinal cancer cells, preventive exposure to flavone apigenin potentiated super-induction of a regulatory ...
Nadeem Uzma - - 2014
MicroRNAs are expressed abundantly in the placenta throughout pregnancy. We have previously reported that miR-378a-5p promoted trophoblast migration and invasion. To further understand the role of miR-378a-5p during placental development, we investigated whether it may regulate the differentiation of syncytiotrophoblast (STB). Using a choriocarcinoma cell line, BeWo, we found that ...
Kim Hye-Young - - 2014
BackgroundHuman immunodeficiency virus-1 (HIV-1) Tat protein plays an essential role in HIV gene transcription from the HIV-1 long terminal repeat (LTR) and replication. Transcriptional activity of Tat is modulated by several host factors, but the mechanism responsible for Tat regulation by host factors is not understood fully.ResultsUsing a yeast two-hybrid ...
Kowsar R R Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, - - 2014
Little is known about the local production and function of alpha 1-acid glycoprotein (AGP), an acute-phase protein, in the female reproductive tract. This study aimed to investigate the regulation and immune function of AGP in cultured bovine oviduct epithelial cells. Analysis by Western blotting and immunohistochemistry revealed that bovine oviduct ...
Van Hoof Dennis D BD Biosciences, San Jose, California, - - 2014
The application of fluorescently-labeled antibodies for flow cytometric identification and characterization of specific cell types within heterogeneous populations by their protein expression profile is well established. While detection of proteins is informative, concomitant transcript analysis in the same cells would provide a more complete and comprehensive view of intracellular signaling ...
Lan Michael S MS The Research Institute for Children, Children's Hospital, New Orleans, LA, USA; Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans, LA, USA. Electronic address: - - 2014
Differentiation of pancreatic endocrine cells depends upon the activation of genes that are regulated by islet transcription factors (ITFs). Evidence suggests that ITFs contribute to the development of the pancreas. These studies are focused on developing a system to deliver individual ITF from different stages of islet cell development to ...
Wróbel Anna Maria AM Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University in Kraków, Gronostajowa 9, 30-387 Krakow, Poland. Electronic address: - - 2014
Numerous studies have shown that widely used parabens possess estrogenic properties. In the present study, we examined the effects of methyl-, propyl- and butylparaben on the mRNA and protein expression of estrogen receptor (ER)-α (ESR1) and -β (ESR2) and the progesterone receptor (PGR). Human MCF-7 breast cancer cells and MCF-10A ...
Aryal Pramod P Department of Pharmacy, College of Pharmacy, Iksan, Jeonbuk, 570-749, Republic of - - 2014
Baicalein, a flavonoid and aglycon hydrolyzed from baicalin, has anticancer properties in several human cancers, but its molecular mechanisms of action remain unclear. Here, we showed that baicalein leads to human cancer cell death by inducing autophagy rather than apoptosis, since cell death induced by baicalein was completely reversed by ...
Matiller V V Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet Litoral), Universidad Nacional del Litoral (UNL)-Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, - - 2014
Cystic ovarian disease (COD) is one of the main causes of infertility in dairy cattle. It has been shown that intra-ovarian factors may contribute to follicular persistence. Transforming growth factor-beta (TGFB) isoforms are important paracrine and autocrine signalling molecules that regulate ovarian follicle growth and physiology. Considering the importance of ...
Barutcu A Rasim AR Department of Cell and Developmental Biology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, - - 2014
Three-dimensional organization of chromatin is fundamental for transcriptional regulation. Tissue-specific transcriptional programs are orchestrated by transcription factors and epigenetic regulators. The RUNX2 transcription factor is required for differentiation of precursor cells into mature osteoblasts. Although organization and control of the bone-specific Runx2-P1 promoter have been studied extensively, long-range regulation has ...
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