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Cui Qing-Ke QK Department of Neurosurgery, The Qilu Hospital of Shandong University, Jinan, Shandong, 250012, P. R. China; Liaocheng People's Hospital, Liaocheng, Shandong, 252000, P. R. - - 2014
To investigate the effect of microRNA (miR-184) on regulating the genesis, development and proliferation of glioma cells. Lipidosome was used to transfect miR-184 mimic and inhibitor to glioma cell line, and the cell proliferation ability changes were determined by MTT and plate cloning experiment after the transfection. WB test was ...
Prochiantz Alain A CIRB, CNRS UMR 7241/INSERM U1050, College de France, 11 place Marcelin Berthelot, Paris Cedex 05 75231, France - - 2014
Homeoprotein (HP) transcription factors were originally identified for their embryonic cell-autonomous developmental functions. In this review, we discuss their postnatal and adult physiological functions based on the study of Otx2, Engrailed-1 and Engrailed-2 (collectively Engrailed). For Engrailed, we discuss its function in the cell-autonomous regulation of ventral midbrain dopaminergic neuron ...
Barmack Neal H NH Department of Physiology and Pharmacology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA - - 2014
Synaptic activation of central neurons is often evoked by electrical stimulation leading to post-tetanic potentiation, long-term potentiation or long-term depression. Even a brief electrical tetanus can induce changes in as many as 100 proteins. Since climbing fibre activity is often associated with cerebellar behavioural plasticity, we used horizontal optokinetic stimulation ...
Chen Jinhui J National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing, 100083, People's Republic of - - 2014
Increasing evidence shows that long non-coding RNAs (lncRNAs) function as important regulatory factors during plant development, but few reports have examined lncRNAs in trees. Here, we report our genome-scale identification and characterization of lncRNAs differentially expressed in the xylem of tension wood, opposite wood and normal wood in Populus tomentosa, ...
Kim Hyun-Ju HJ Department of Molecular Medicine, Cell and Matrix Research Institute, Clinical Trial Center, School of Medicine,Kyungpook National University, Daegu 700-422, Korea, Skeletal Diseases Genome Research Center, Kyungpook National University Hospital, Daegu 700-412, - - 2014
Osteoclasts are large polykaryons that have the unique capacity to degrade bone and are generated by the differentiation of myeloid lineage progenitors. To identify the genes involved in osteoclast development, we performed microarray analysis, and we found that carboxypeptidase E (CPE), a prohormone processing enzyme, was highly upregulated in osteoclasts ...
Song Lei L State Key Laboratory of Cardiovascular Diseases, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, - - 2014
The molecular mechanisms that drive the development of cardiac hypertrophy in hypertrophic cardiomyopathy (HCM) remain elusive. Accumulated evidence suggests that microRNAs are essential regulators of cardiac remodelling. We have been suggested that microRNAs could play a role in the process of HCM. To uncover which microRNAs were changed in their ...
Hug Nele N MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, - - 2014
Nonsense-mediated decay (NMD) is a surveillance mechanism that degrades aberrant mRNAs. A complex comprising SMG1, UPF1, and the translation termination factors eRF1 and eRF3 (SURF) is assembled in the vicinity of a premature termination codon. Subsequently, an interaction with UPF2, UPF3b, and the exon junction complex induces the formation of the decay-inducing complex (DECID) ...
Zuo Bin B Department of Orthopedic Surgery, Xinhua Hospital, Shanghai JiaoTong University School of Medicine (SJTUSM), Shanghai, - - 2014
Emerging evidence indicates that microRNAs (miRNAs) play essential roles in regulating osteoblastogenesis and bone formation. However, the role of miRNA in osteoblast mechanotransduction remains to be defined. In this study, we aimed to investigate if miRNAs regulate mechanical stimulation-triggered osteoblast differentiation and bone formation through modulation of Runx2, the master ...
Dwyer Joseph J Randall Division of Cell and Molecular Biophysics and Cardiovascular Division, British Heart Foundation Centre of Research Excellence, King's College London, SE1 1UL, United - - 2014
Members of the formin family are known to be involved in the regulation of the actin cytoskeleton. We have recently identified a muscle specific splice variant of the formin FHOD3 and demonstrated its role in the maintenance of the contractile filaments of cardiomyocytes. Here, we characterize the expression and subcellular ...
Tripathi Subhash K SK Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland; National Doctoral Programme in Informational and Structural Biology, Turku, Finland; Turku Doctoral Programme of Molecular Medicine (TuDMM), University of Turku, Turku, - - 2014
Combined with TCR stimuli, extracellular cytokine signals initiate the differentiation of naive CD4(+) T cells into specialized effector T-helper (Th) and regulatory T (Treg) cell subsets. The lineage specification and commitment process occurs through the combinatorial action of multiple transcription factors (TFs) and epigenetic mechanisms that drive lineage-specific gene expression ...
Di Santo James P JP Innate Immunity Unit, Institut Pasteur, Paris, France; Inserm U668, Institut Pasteur, Paris, - - 2014
Innate and adaptive lymphocytes are characterized by phenotypic and functional characteristics that result from genomic rearrangements (in the case of antigen-specific B and T cells) coupled with selective gene expression patterns that are generated in a context-dependent fashion. Cell-intrinsic expression of transcription factors (TFs) play a critical role in the ...
Imanaka-Yoshida Kyoko K Department of Pathology and Matrix Biology, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan; Mie University Research Center for Matrix Biology, Tsu, Mie, 514-8507, - - 2014
Tenascin-C (TNC) is an extracellular glycoprotein categorized as a matricellular protein. It is highly expressed during embryonic development, wound healing, inflammation, and cancer invasion, and has a wide range of effects on cell response in tissue morphogenesis and remodeling including the cardiovascular system. In the heart, TNC is sparsely detected ...
Shan Ren Feng RF Department of General Surgery, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. - - 2014
In the present study, the effect of Aurora-B inhibition on HepG2 cell invasion and migration in vitro was investigated. A recombinant plasmid targeting the Aurora-B gene (MiR-Aurora-B) was used to inhibit Aurora-B expression in HepG2 cells. Cell migration and invasion were investigated using Transwell migration and invasion assays. The results ...
Guo Zhentao Z Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, - - 2014
Leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) are an inhibitor of receptor tyrosine kinases (RTKs) that was discovered in recent years, and many studies showed that LRIG1 is a tumor suppressor gene and may be related to tumor drug resistance. In this study, we explored whether LRIG1 protein expression can ...
Sung Hye Youn HY Department of Biochemistry, School of Medicine, Ewha Womans University, Seoul, - - 2014
Recent discoveries suggest that aberrant DNA methylation provides cancer cells with advanced metastatic properties. However, the precise regulatory mechanisms controlling metastasis genes and their role in metastatic transformation are largely unknown. To address epigenetically-regulated gene products involved in ovarian cancer metastasis, we examined the mechanisms regulating mucin 13 (MUC13) expression ...
Kotla Sivareddy S Department of Physiology, University of Tennessee Health Science Center, 894 Union Avenue, Memphis, TN 38163, - - 2014
15(S)-Hydroxyeicosatetraenoic acid (15(S)-HETE), the major 15-lipoxygenase 1/2 (15-LO1/2) metabolite of arachidonic acid (AA), induces CD36 expression through xanthine oxidase and NADPH oxidase-dependent ROS production and Syk and Pyk2-dependent STAT1 activation. In line with these observations, 15(S)-HETE also induced foam cell formation involving ROS, Syk, Pyk2 and STAT1-mediated CD36 expression. In ...
Hu Biao B University of Michigan, Pathology , BSRB 4830 , 109 Zina Pitcher Place , Ann Arbor, Michigan, United States, 48109 , 734-763-5731 , 734-615-2331 ; - - 2014
Hedgehog signaling plays important roles in development and cell differentiation. In this study, the ability of sonic Hedgehog (SHH) to induce myofibroblast differentiation was analyzed in isolated human lung fibroblasts and its in vivo significance was evaluated in rodent bleomycin induced pulmonary fibrosis. The results showed that SHH could induce ...
Karalok Hakan Mete HM Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine, New Haven, - - 2014
Background: Cytokines and growth factors play important roles in endometrial function and the pathogenesis of endometriosis. Messenger RNAs (mRNAs) encoding cytokines and growth factors undergo rapid turnover; primarily mediated by AU-rich elements (ARE) located in their 3'-untranslated regions (3'-UTRs). T-cell intracellular antigen (TIA-1), an mRNA-binding protein, binds to AREs in ...
Taleb Shaghayegh S Department of Biology, Sciences and Research Branch, Islamic Azad University, Tehran, - - 2014
Notch signaling is one of the main involved pathways in cell differentiation and organogenesis, and its deregulation may lead to tumorigenesis. In this pathway, targeted to the CSL (CBF1, Suppressor of Hairless or Lag-1) complex, notch intracellular domain (NICD) releases corepressors and recruits MAML1 as coactivator triggering the activation of ...
Yamamoto-Fukuda Tomomi T Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan, - - 2014
We reported previously that keratinocyte growth factor (KGF), a mesenchymal cell-derived paracrine growth factor, plays an important role in middle ear cholesteatoma formation, which is characterized by marked proliferation of epithelial cells. Here, we investigated whether KGF, the main factor that induces cholesteatoma, overexpression in vivo results in the formation ...
Xu Xiaofeng X College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, - - 2014
Prechordal mesendoderm (PME) is a derivative of gastrula organizer underlying the anterior neural plate of vertebrate embryos. It has been firmly established that PME is critical for head induction and anterior-posterior patterning. Therefore, the establishment of PME in a desired shape and size at a correct position during early embryogenesis ...
Wu Shey-Lin SL Department of Neurology, Chang-Hua Christian Hospital, Changhua, Taiwan, Republic of - - 2014
Homeobox genes encode transcription factors that regulate embryonic development programs including organogenesis, axis formation and limb development. Previously, we identified and cloned a mouse double homeobox gene, Duxbl, whose homeodomain exhibits the highest identity (67 %) to human DUX4, a candidate gene of facioscapulohumeral muscular dystrophy (FSHD). Duxbl proteins have been ...
Huang Kuo-Hsiung - - 2014
BackgroundOur previous study showed NF-¿B repressing factor (NKRF) downregulates IP-10 and IL-8 synthesis in the peripheral blood mononuclear cells and alveolar macrophages of TB patients with high bacterial loads. However, the mechanism underlying the repressive effect of NKRF is not fully understood.ResultsThe levels of IP-10, IL-8 and NKRF were significantly ...
Zhao Guangfeng - - 2014
BackgroundMesenchymal stem cells (MSCs) at maternal-fetal interface are considered to play an important role in the pathogenesis of pre-eclampsia (PE). microRNAs (miRNAs) also have an important influence on differentiation, maturation, and functions of MSCs. Our aim in this study was to determine the differential expression of miRNAs in decidua-derived MSCs ...
Lu Bin B College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei, People's Republic of - - 2014
Altered gene expression coincides with leukemia development and may affect distinct features of leukemic cells. PITHD1 was significantly downregulated in leukemia and upregulated upon PMA induction in K562 cells undergoing megakaryocyte differentiation. We aimed to study the function of PITHD1 in megakaryocyte differentiation. K562 cells and fetal liver cells were ...
Diaz-Rodriguez Esther E CIMUS (E.D.-R., A.R.G.-G., M.G.-L., C.D., C.V.A.), University of Santiago de Compostela, and Department of Endocrinology (I.B.), University Hospital (CHUS), IDIS, Santiago de Compostela, Spain; Departments of Cell Biology, Physiology, and Immunology (A.I.-C., E.G.-P., R.M.L., J.P.C.), and Morphological Sciences (L.J.-R.), University of Cordoba, and Reina Sofia University Hospital (P.B., M.A.G.), Maimonides Institute for Research in Biomedicine of Cordoba, Córdoba, Spain; Departments of Endocrinology and Pathology (A.L., M.A.J., A.S., E.V.), Hospital Universitario Virgen del Rocío, IBIS-CSIC, University of Sevilla, Sevilla, Spain; Department of Endocrinology (F.J.T.), Hospital Virgen de la Victoria, and Department of Endocrinology (J.A.G.-A.), Hospital Carlos Haya, Malaga, Spain; and CIBERObn Network (A.I.-C., A.L., F.J.T., P.B., L.J.-R., I.B., C.D., R.M.L., J.P.C., - - 2014
Acromegaly is caused by somatotroph cell adenomas (somatotropinomas [ACROs]), which secrete GH. Human and rodent somatotroph cells express the RET receptor. In rodents, when normal somatotrophs are deprived of the RET ligand, GDNF, RET is processed intracellularly to induce overexpression of Pit1, which in turn leads to p19Arf/p53-dependent apoptosis. Our ...
Tribolet Leon L Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD 4878, - - 2014
Na-ASP-2 is an efficacious hookworm vaccine antigen; however, despite elucidation of its crystal structure and studies addressing its immunobiology, the function of Na-ASP-2 has remained elusive. We probed a 9,000 protein human proteome microarray with Na-ASP-2 and showed binding to CD79A, a component of the B cell antigen receptor complex. ...
Hlaing Su M SM S Hlaing, Internal Medicine, Charles R. Drew University, Los Angeles, United - - 2014
Cardiovascular disease (CV) remains the leading cause of death worldwide. Low levels of vitamin D are associated with high risk of myocardial infarction, even after controlling for factors associated with coronary artery disease. A growing body of evidence suggests that vitamin D plays an important role in CV related signaling ...
Man Pui-Sin PS P Man, Biosciences, Cardiff University, Cardiff, United - - 2014
The transcription factor gene, Egr1, is necessary for female fertility; EGR1 protein is an established molecular regulator of adult female gonadotroph function where it mediates GnRH-stimulated transcription of the Lhb gene. Recent studies have also implicated pituitary EGR1 in the mediation of other physiological signals suggesting an integrative function. However, ...
Bao Zhao-Shi ZS Beijing Neurosurgical - - 2014
Studies of gene rearrangements and the consequent oncogenic fusion proteins have laid the foundation for targeted cancer therapy. To identify oncogenic fusions associated with glioma progression, we catalogued fusion transcripts by RNA-seq of 272 WHO grade II, III, and IV gliomas. Fusion transcripts were more frequently found in high grade ...
Balestra Fernando R FR Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, - - 2014
During differentiation of multiciliated cells, numerous centrioles are generated in each cell to act as templates for the formation of a corresponding number of cilia. A new study reveals that multicilin, a protein required for multiciliogenesis, is a key component of a regulatory complex that activates the transcription of genes ...
Beilfuss Anja A Department of Gastroenterology and Hepatology, University Hospital, University Duisburg-Essen, Essen, - - 2014
Non-alcoholic fatty liver disease (NAFLD) is closely linked to obesity and constitutes part of the metabolic syndrome, which have been associated with low serum vitamin D (VD). Due to known crosstalk between VD and transforming growth factor (TGF)-β signalling, VD has been proposed as an antifibrotic treatment. We evaluated the ...
Watanabe Yukiko Y Departments of - - 2014
Epigenetic modifications, such as posttranslational modifications of histones, play an important role in gene expression and regulation. These modifications are in part mediated by the Trithorax group (TrxG) complex and the Polycomb group (PcG) complex, which activate and repress transcription, respectively. We herein investigate the role of Menin, a component ...
Lai Jing J Department of Medical Oncology, Jinling Hospital, School of Medicine, Southern Medical University, Guangzhou, 510282, - - 2014
The p73 gene contains an extrinsic P1 promoter and an intrinsic P2 promoter, controlling the transcription of the pro-apoptotic TAp73 isoform and the anti-apoptotic ΔΝp73 isoform, respectively. The DNA methylation status of both promoters act equally in the epigenetic transcriptional regulation of their relevant isoforms. The aim of this study ...
Lee Su Ui SU Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk 363-883, - - 2014
The beta2 adrenergic receptor (ADRB2) is a G protein-coupled transmembrane receptor expressed in the human respiratory tract and widely recognized as a pharmacological target for treatments of asthma and chronic obstructive pulmonary disorder (COPD). Although a number of ADRB2 agonists have been developed for use in asthma therapy, indacaterol is ...
Samuel Temesgen - - 2014
The nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB) signaling pathway is activated in cells exposed to various stimuli, including those originating on the cell surface or in the nucleus. Activated NF-kappaB signaling is thought to enhance cell survival in response to these stimuli, which include chemotherapy and radiation. In ...
Agrawal Rahul - - 2014
Hypoxia is a critical aspect of the glioma microenvironment and has been associated with poor prognosis and resistance to various therapies. However, the mechanisms responsible for hypoxic survival of glioma cells remain unclear. Recent studies strongly suggest that microRNAs act as critical mediators of the hypoxic response. We thus hypothesized ...
Leite Fábio Renato Manzolli FR School of Dentistry, Federal University at Pelotas (UFPel), Pelotas, RS, - - 2014
The myeloid differentiation factor 88 (MyD88) plays a pivotal role in Toll-like receptor (TLR)- and interleukin-1 receptor (IL-1R)-induced osteoclastogenesis. We examined the role of MyD88 on p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) activation and nucleotide-binding oligomerization domain (Nod) induction by lipopolysaccharide (LPS) ...
Zhou Jianbiao J Cancer Science Institute of Singapore, National University of Singapore, 14 Medical Drive, Centre for Translational Medicine, Singapore 117599, Republic of - - 2014
Over-expression of PRL-3 has been identified in about 50% of patients with acute myeloid leukemia (AML). The mechanism of regulation of PRL-3 remains obscure. Signal transducer and activator of transcription 3 (STAT3), a latent transcriptional factor, has also been often found to be activated in AML. We first identified consensus ...
Wang Zheng-Yu ZY Department of Surgery, Indiana University School of Medicine, MS B005, 635 Barnhill Dr., Indianapolis, IN 46202, United States. Electronic address: - - 2014
Kupffer cells (KC) play a critical role in both liver physiology and the pathogenesis of various liver diseases. Isolated primary KC have a limited lifespan in culture, and due to the relatively low number obtained, limit their study in vitro. Here, a cytokine-producing immortalized KC (ImKC) line was established from ...
Cox Megan K MK Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL 35294-0005. Electronic address: - - 2014
Transforming growth factor β (TGF-β) signaling plays an integral role in skeletal development. Conditional deletion of the TGF-β type II receptor (Tgfbr2) from Type II Collagen (Col2a) expressing cells results in defects in development of the annulus fibrosus (AF) of the intervertebral disc (IVD). We previously used microarray analysis to ...
Srivastava Jyoti J Department of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, - - 2014
Retinoid X receptor (RXR) regulates key cellular responses such as cell growth and development, and this regulation is frequently perturbed in various malignancies, including hepatocellular carcinoma (HCC). However, the molecule(s) that physically govern this deregulation are mostly unknown. Here, we identified RXR as an interacting partner of astrocyte-elevated gene-1 (AEG-1)/metadherin ...
Schönitzer Veronika V 1 Experimental Surgery and Regenerative Medicine, Department of Surgery, Ludwig-Maximilians-University , 80336, Munich, Germany - - 2014
Abstract Human mesenchymal stem cells (hMSCs) are a promising target for cell-based bone regeneration. However, their application for clinical use is limited because hMSCs lose their ability for cell division and differentiation during longer in vitro cultivation. The osteogenic differentiation is regulated through a complex network of molecular signal transduction ...
Penumatsa Krishna C KC Tufts Medical - - 2014
We previously reported that transglutaminase 2 (TG2) activity is markedly elevated in lungs of hypoxia-exposed rodent models of pulmonary hypertension (PH). Since vascular remodeling of pulmonary artery smooth muscle cells (PASMCs) is important in PH, we undertook the present study to determine if TG2 activity is altered in PASMCs with ...
Fang Yong - - 2014
p27Kip1 is a potent inhibitor of cyclin-dependent kinases that drive G1 to S cell-cycle transition and reduced p27Kip1 expression is prevalent in a wide range of human tumors, the exact mechanism(s) of p27Kip1-mediated tumor suppression remains obscure. In this study, we identified a close, inverse relationship between p27Kip1 and EGFR ...
Bohinc Brittany N BN Division of Endocrinology, Diabetes, and Metabolism, Duke University, Durham, - - 2014
Thyroid hormone (TH) is important for tissue repair because it regulates cellular differentiation. Intrahepatic TH activity is controlled by both serum TH levels and hepatic deiodinases. TH substrate (thyroxine, T4) is converted into active hormone (triidothyronine, T3) by deiodinase 1 (D1), but into inactive hormone (reverse T3, rT3) by deiodinase ...
Zhong Zhaopeng Z Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. - - 2014
MicroRNA (miR)‑30c has been identified as a tumor suppressor gene in numerous diseases. Aberrant miR‑30c expression has been associated with the invasion of different types of cancer. However, the potential mechanisms underlying the association between miR‑30c and invasion has been poorly elucidated in non‑small‑cell lung cancer (NSCLC). In the present ...
Song Zhenguo Z Department of Pancreatic Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, China, - - 2014
The aim of study was to discuss the correlation and regulatory mechanism of HIF-1 and miR-191 expression in pancreatic tumor. The association between the miR-191 and the clinicopathologic characteristics and the prognosis of pancreatic cancer was further explored. After hypoxic cultured for 6 and 12 h, qRT-PCR and Western blot were ...
Hu Dan-Ning DN Tissue Culture Center, New York Eye and Ear Infirmary, New York Medical College, New York, NY, 10003, United States - - 2014
Purpose: Melanocytes are one of the major cellular components in the uvea. IL-8/CXCL8 and monocyte chemoattractant protein-1 (MCP-1/CCL2) are the two most important pro-inflammatory chemokines. We studied the constitutive and lipopolysaccharide (LPS)-induced expression of IL-8 and MCP-1 in cultured human uveal melanocytes (UM), and explored the relevant signal pathways. Methods: ...
Dabir Snehal S Division of Hematology/Oncology, Case Western Reserve University/University Hospitals of - - 2014
Purpose: Deregulation of STAT3 activation is a hallmark of many cancer cells and the underlying mechanisms are subject to intense investigation. We examined the extent of PIAS3 expression in mesothelioma cells and human tumor samples and determined the functional effects of PIAS3 expression on STAT3 signaling. Experimental design: We evaluated ...
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