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Wang Chunmei C Neurobiology Institute, Jining Medical University, Jining 272067, P.R. - - 2014
MicroRNAs (miRNAs) are small, endogenous, non-coding RNA molecules that function as post-transcriptional regulators of gene expression by imperfect base-pairing with the 3'-untranslated regions of their target mRNAs. Altered expression of numerous miRNAs has been shown to be extensively involved in the pathogenesis of various diseases and cancers. Additionally, the specific ...
Xie Ning N The Vancouver Prostate Centre, Department of Urologic Sciences, University of British Columbia, Vancouver, - - 2014
The glucocorticoid and androgen receptors (GR and AR) can commonly regulate up to 50% of their target genes in prostate cancer (PCa) cells. GR expression is stimulated by castration therapy, which has been proposed to be one mechanism that compensates for AR signaling blockade and promotes castration-resistant PCa (CRPC) progression. ...
Balke Darko D Ernst-Moritz-Arndt Universität Greifswald, Institut für Biochemie, Felix-Hausdorff-Strasse 4, 17478 Greifswald - - 2014
RNA repair is an emerging strategy for gene therapy. Conventional gene therapy typically relies on the addition of the corrected DNA sequence of a defective gene to restore gene function. As an additional option, RNA repair allows alteration of the sequence of endogenous messenger RNAs (mRNAs). mRNA sequence alteration is ...
Griseri Paola P Paola Griseri, United Orthopedic Corporation, Genetica Medica, Institute Giannina Gaslini, 16148 Genoa, - - 2014
The control of the half-life of mRNA plays a central role in normal development and in disease progression. Several pathological conditions, such as breast cancer, correlate with deregulation of the half-life of mRNA encoding growth factors, oncogenes, cell cycle regulators and inflammatory cytokines that participate in cancer. Substantial stability means ...
Ichiki Tomoko T Division of Cardiovascular Disease, Mayo Clinic, Rochester, MN, USA. Electronic address: - - 2014
Left ventricular assist device (LVAD) support has been used in the treatment of end-stage heart failure (HF), however use of anti-fibrotic co-therapies may improve prognosis. Natriuretic peptides (NPs) possess anti-fibrotic properties through their receptors, GC-A/GC-B/NPR-C. We sought to evaluate cardiac fibrosis and the endogenous NP system in end-stage HF with ...
Tanaka Satoshi S Department of Neuro-Oncology and Neurosurgery, Tokyo Nishi Tokushukai - - 2014
Object Methylation of O(6)-methylguanine-DNA methyltransferase (MGMT) has been reported to be a good prognostic factor for patients with glioblastoma multiforme (GBM). To determine whether the absolute value of MGMT messenger RNA (mRNA) might be a prognostic factor and useful for predicting the therapeutic effectiveness of temozolomide, especially with regard to ...
Kim Mi Jin MJ Department of Pediatrics, Ilsan Paik Hospital, Inje University College of Medicine, Goyang, - - 2014
This study investigated the expression of T cell immunoglobulin- and mucin-domain-containing molecule 3 (TIM-3), human β-defensin (HBD)-2, forkhead box protein 3 (FOXP3), and the frequency of CD4+ CD25+ FOXP3+ regulatory T cells (Tregs) in children with Crohn's disease (CD) during infliximab therapy. We enrolled 20 CD patients who received infliximab ...
Bueno da Silva Henrique Aparecido HA Division of Periodontics, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, SP, - - 2014
Background: Protease activated receptor 1 (PAR1) activation by thrombin may play a role in repair and homeostasis of periodontal tissues. The main objective of this study was to investigate PAR1 expression in periodontitis patients, before and after non-surgical periodontal treatment, and to associate its expression with the presence of inflammatory ...
Liu H B HB Australian Centre for Blood Diseases, Monash University, Prahran 3181, Melbourne, Victoria, - - 2014
Combination epigenetic treatment (EGT) utilizing DNA methyl transferase inhibitors (DNMTi) and histone deacetylase inhibitors (HDACi) may be more efficacious than single agent treatment in myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). The molecular mechanisms behind the potential clinical efficacy of combination EGT treatment are incompletely understood and the frequently ...
Wang Youwei - - 2014
BackgroundTo study the expression of D2R, MGMT and VEGF for clinical significance in pituitary adenomas, and to predict the potential curative medical therapy of dopamine agonists, temozolomide and bevacizumab on pituitary adenomas.MethodsImmunohistochemistry and western blot were performed to detect the expression of expression of D2R, MGMT and VEGF in pituitary ...
Gammons M V MV Microvascular Research Laboratories, School of Physiology and Pharmacology, Preclinical Veterinary Sciences Building, University of Bristol, Southwell Street, Bristol BS2 8EJ, - - 2014
Background:Current therapies for metastatic melanoma are targeted either at cancer mutations driving growth (e.g., vemurafenib) or immune-based therapies (e.g., ipilimumab). Tumour progression also requires angiogenesis, which is regulated by VEGF-A, itself alternatively spliced to form two families of isoforms, pro- and anti-angiogenic. Metastatic melanoma is associated with a splicing switch ...
Stary Creed M CM Department of Anesthesia, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA, 94305, - - 2014
Astrocytes are critical regulators of neuronal function and an effective target for stroke therapy in animal models. Identifying individual targets with the potential for simultaneous activation of multiple downstream pathways that regulate astrocyte homeostasis may be a necessary element for successful clinical translation. Mitochondria and microRNAs each represent individual targets ...
Young Robin J RJ Academic Unit of Clinical Oncology, Weston Park Hospital, Whitham Rd, Sheffield S10 2SJ, UK; Academic Unit of Surgical Oncology, Medical School, Beech Hill Rd, University of Sheffield, Sheffield S10 2RX, UK. Electronic address: - - 2014
Angiosarcomas are rare malignant vascular tumours. Angiosarcoma expression of vascular endothelial growth factor (VEGF) has previously been reported, but angiosarcoma expression of other angiogenic growth factors has not been systematically studied. Non-VEGF angiogenic growth factors are a potential mechanism of resistance to VEGF-targeted therapy, but they also represent potential therapeutic ...
Kendrick Samantha L SL Department of Pathology, University of Arizona, Tucson, AZ - - 2014
Patients with aggressive, BCL2 protein-positive (+) diffuse large B-cell lymphoma (DLBCL) often experience rapid disease progression that is refractory to standard therapy. However, there is potential for false-negative staining of BCL2 using the standard monoclonal mouse 124 antibody that hinders the identification of these high-risk DLBCL patients. Herein, we compare ...
Ogihara Yoshihito Y Department of Pediatrics, Kitasato University School of Medicine, Sagamihara, Kanagawa, - - 2014
BACKGROUND:Infliximab (IFX) is a known monoclonal antibody against TNF-α that is used to treat Kawasaki disease (KD) patients with intravenous immunoglobulin (IVIG) resistance. The transcriptional modulation of inflammation following IFX therapy has not been reported in KD patients.METHODS:We investigated the transcript abundance profiles in whole blood obtained from eight IVIG-resistant ...
Carlile Graeme W GW Cystic Fibrosis Translational Research Center, Dept. of Biochemistry, McGill University, Montreal, Quebec H3G1Y6, Canada. Electronic address: - - 2014
Small molecules as shown by VX809 can rescue the mislocalization of F508del-CFTR. The aim of this study was to identify correctors with a clinical history and their targets of action. CFTR correctors were screened using two F508del-CFTR expressing cell based HTS assays. Electrophysiological studies using CFBE41o(-) and HBE cells and ...
Zheng Zhenlong Z Department of Dermatology, Yanbian University Hospital, Yanji City, Jilin Province, - - 2014
Runt-related transcription factor 3 (RUNX3) has recently been reported to be a possible predictor of sensitivity of cancer cells for photodynamic therapy (PDT), a promising therapeutic modality for keloids. In this study, we aimed to elucidate the implications of RUNX3 for keloid pathogenesis and sensitivity to pheophorbide a-based PDT (Pa-PDT). ...
Majhi Arnab A Department of Physiology, Immunology laboratory. University of Calcutta, University Colleges of Science and Technology, 92 APC Road, Calcutta-700009, West Bengal, - - 2014
Whether synergistic antimicrobial combination in vitro would be beneficial in down regulation of pneumococcal virulent genes and associated inflammation of the lung tissue induced by multi-drug resistant S. pneumoniae infection in vivo needs to be elucidated for considering this mode of therapy in case of severe pneumococcal infection. We investigated ...
Kim Joong Sun - - 2014
This study evaluated the detrimental effect of β-emission on pig skin model. Skin injury was modeled in mini pigs by exposing them to 50 and 100 Gy of β-emission delivered from holmium-166 patches. Clinicopathological and immunohistochemical changes in exposed skin were monitored for 18 weeks after β-irradiation. β-irradiation induced desquamation ...
Giza Dana Elena DE The University of Texas MD Anderson Cancer Center, Department of Experimental Therapeutics , Unit 1950, 1515 Holcombe Blvd, Houston, TX, 77030-4009 , USA +1 713 792 5461 ; +1 713 792 1204 ; - - 2014
Introduction: A new concept of gene regulation, in which competitive endogenous RNAs (ceRNAs) compete for common microRNAs (miRNAs), suggests that mRNA targets have an active role as key elements in the regulation of miRNA availability within cells. ceRNAs are considered to be natural decoys of miRNA activity and can influence ...
Wang Jian J Department of Gastrointestinal Oncology; H. Lee Moffitt Cancer Center & Research Institute; Tampa, FL USA; Department of Pancreatic Oncology; Cancer Institute and Hospital of Tianjin Medical University; Tianjin, PR - - 2014
HPP1 (hyperplastic polyposis protein 1), a tumor suppressor gene, is downregulated by promoter hypermethylation in a number of tumor types including colon cancer. c-Myc is also known to play a role in the suppression of HPP1 expression via binding to a promoter region cognate E-box site. The contribution of histone ...
Borovaya Alena A Department of Dermatology and Allergology, Ludwig Maximilian University Munich, Frauenlobstr. 9-11, 80337, Munich, - - 2014
In acne vulgaris, antimicrobial peptides (AMPs) could play a dual role; i.e., protective by acting against Propionibacterium acnes, pro-inflammatory by acting as signalling molecules. The cutaneous expression of 15 different AMPs was investigated in acne patients; furthermore, the impact of isotretinoin therapy on AMP expression was analysed in skin biopsies ...
Salama Mohamed E - - 2014
Abstract Little is known about aberrant antigens expression and their association with cytogenetic aberrations in multiple myeloma (MM). We examined the correlation between flow cytometry (FC), and FISH in 167 marrow specimens with MM. Gene expression profiling of CD56, CD117, CD52 and CD20 mRNA in plasma cells (PCs) from total ...
Köhler Andrea - - 2014
High androgen receptor (AR) level in primary tumour predicts increased prostate cancer (PCa)-specific mortality. Furthermore, activations of the AR, PI3K, mTOR, NFkappaB and Hedgehog (Hh) signaling pathways are involved in the fatal development of castration-resistant prostate cancer during androgen ablation therapy. MID1, a negative regulator of the tumor-suppressor PP2A, is ...
Gao Wei - - 2014
Curcumin suppressed proliferation, migration, invasion, metastasis, and angiogenesis and induced apoptosis by regulating multiple signaling pathways and miRNAs in a wide variety of human malignancies. miRNAs play crucial roles in various steps of carcinogenesis in NPC, thus miRNAs could serve as critical therapeutic targets for NPC treatment. Curcumin could provide ...
Yanagisawa Nobuyuki N Department of Pathology, Kitasato University School of Medicine, Kanagawa, 252-0374, Japan; Department of Pathology of Biological Responses, Kitasato University Graduate School of Medical Science, Kanagawa, 252-0374, - - 2014
Oncocytic L-type amino acid transporter (LAT) 1 may be a prognostic indicator and target of new molecular therapeutic agents against malignancies. To investigate whether LAT1 expression influence the outcomes of patients with bile duct cancer, the expression of LAT1, LAT2, CD98, and Ki-67 was investigated immunohistochemically in 134 surgically resected ...
Jia Gq G Center for Nutrition and Pregnancy, Animal Sciences Department, North Dakota State University, Fargo, United States of - - 2014
: Maternal uterine artery adenovirus VEGF (Ad. VEGF-A165) gene therapy has increased pre- and early postnatal growth velocity in an overnourished adolescent ovine model of fetal growth restriction. Our objective was to investigate effects of prenatal Ad. VEGF on fetal and postnatal intestinal mRNA expression of angiogenic factors and receptors ...
Jia Gq G Center for Nutrition and Pregnancy, Animal Sciences Department, North Dakota State University, Fargo, United States of - - 2014
: Maternal uterine artery adenovirus VEGF (Ad. VEGF-A165) gene therapy has increased pre- and early postnatal growth velocity in an overnourished adolescent ovine model of fetal growth restriction. Our objective was to investigate effects of prenatal Ad. VEGF on fetal and postnatal intestinal mRNA expression of angiogenic factors and receptors ...
Sepehrizadeh Zargham Z Tehran University of Medical Sciences Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Tehran, Iran ; Tehran University of Medical Sciences, Biotechnology Research Center, Tehran, - - 2014
One of the major goals of cancer treatment is the monitoring of chemotherapeutic protocols. Quantitative and comparative cytokine expression profiling could be reliable to be used for biomarkers in deadly and fast-growing cancers such as acute myeloid leukemia (AML). The present study aims to assess and further validate cytokines with ...
Sardu Celestino C Department of Medical, Surgical, Neurological, Metabolic & Geriatric Sciences, Second University of Naples, Piazza Miraglia, 2, 80138, Naples, - - 2014
Heart failure (HF) disease progression is related to numerous adaptive processes including cardiac fibrosis, hypertrophy and apoptosis by activation of the 'fetal' gene program and downregulation of mRNA signatures, suggesting the importance of molecular mechanisms that suppress mRNA steady-state levels. miRNAs (miRs) are small, noncoding RNAs that bind mRNAs at ...
Muñoz-Culla M M Biodonostia Health Research Institute, San Sebastián, SpainSpanish network on Multiple - - 2014
Natalizumab has shown its efficacy in reducing multiple sclerosis (MS) relapses and progression of disability; however, it has been associated with an increased risk of developing progressive multifocal leukoencephalopathy (PML). The differential expression of microRNA (miRNA), the small non-coding RNAs that regulate gene expression, in natalizumab-treated patients has been reported ...
Riquelme Erick E Translational Molecular Pathology, The University of Texas MD Anderson Cancer - - 2014
Purpose: Investigate the mechanisms of regulation and role associated with EZH2 expression in lung cancer cells. Experimental Design: We investigated the mechanisms of EZH2 expression associated with the vascular endothelial growth factor (VEGF)/VEGF receptor 2 (VEGFR-2) pathway. Furthermore, we sought to determine the role of EZH2 in response of lung ...
Peng Xinxia X Department of Microbiology, University of Washington, Seattle 98109, USA Washington National Primate Research Center, Seattle 98109, - - 2014
Polyadenylated mature mRNAs are the focus of standard transcriptome analyses. However, the profiling of nascent transcripts, which often include non-polyadenylated RNAs, can unveil novel insights into transcriptional regulation. Here, we separately sequenced Total RNAs (Total RNAseq) and mRNAs (mRNAseq) from the same HIV-1 infected human CD4+ T cells. We found ...
Shimada Tsutomu T Faculty of Pharmacy, Musashino University, 1-1-20 Shinmachi, Nishitokyo, Tokyo, 202-8585, Japan, - - 2014
Salacia reticulata Wight (S. reticulata) is a herbal medicine used for treatment of early diabetes in Ayurvedic medicine. In previous reports, the extract of S. reticulata showed preventive effects on obesity and various metabolic disorders and a suppressive effect on differentiation in premature adipocytes. The aim of this research was ...
Lu Min M Ludwig Institute for Cancer Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford OX3 7DQ, - - 2014
The tumour suppressor p53 is a master sensor of stress and it controls the expression of hundreds to thousands of genes with diverse biological functions including cell cycle arrest, apoptosis, and senescence. Consequently p53 is the most mutated gene found in human cancer and p53 mutation rate varies from 5% ...
De Felice Bruna - - 2014
Non-coding small RNA molecules play pivotal roles in cellular and developmental processes by regulating gene expression at the post-transcriptional level. In human diseases, the roles of the non-coding small RNAs in specific degradation or translational suppression of the targeted mRNAs suggest a potential therapeutic approach of post-transcriptional gene silencing that ...
Stewart Grant D GD Edinburgh Urological Cancer Group, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK; Scottish Collaboration On Translational Research into Renal Cell Cancer, - - 2014
There is a lack of biomarkers to predict outcome with targeted therapy in metastatic clear cell renal cancer (mccRCC). This may be because dynamic molecular changes occur with therapy. To explore if dynamic, targeted-therapy-driven molecular changes correlate with mccRCC outcome. Multiple frozen samples from primary tumours were taken from sunitinib-naïve ...
Safe Stephen S Texas A&M University, Veterinary Physiology and Pharmacology , 4466 TAMU, College Station, TX 77843-4466 , USA - - 2014
Introduction: Specificity protein (Sp) transcription factors (TFs) are members of the Sp/Kruppel-like factor family, and Sp proteins play an important role in embryonic and early postnatal development. Sp1 has been the most extensively investigated member of this family, and expression of this protein decreases with age, whereas Sp1 and other ...
Manceau Gilles G UMR-S775, Université Paris - - 2014
To identify miRNAs that predict response to anti-EGFR antibodies in wild-type KRAS metastatic colorectal cancer (mCRC) patients. microRNA profiling was performed in a training set of 87mCRC patients refractory to chemotherapy treated with anti-EGFR antibodies. This included 33 fresh frozen (FF) and 35 formalin fixed paraffin embedded (FFPE) samples retrospectively ...
Fan Jin-Zhu JZ Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, People's Republic of - - 2014
Estrogen deficiency is the main reason of bone loss, leading to postmenopausal osteoporosis, and estrogen replacement therapy (ERT) has been demonstrated to protect bone loss efficiently. Notch signaling controls proliferation and differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). Moreover, imperfect estrogen-responsive elements (EREs) were found in the 5'-untranslated region ...
Jelkmann Wolfgang W Institute of Physiology, University of Luebeck, Luebeck, - - 2014
Recombinant human erythropoietin (rhEpo, epoetin) has proved beneficial in preventing transfusion-dependent anaemia in patients with chronic kidney disease. Apart from copied epoetins distributed in less regulated markets, 'biosimilar' epoetins have gained currency in many regions, where they compete with the originals and with rhEpo analogues with prolonged survival in circulation ...
Januszyk Michael M Department of Surgery; Division of Plastic and Reconstructive Surgery; Stanford University School of Medicine; Stanford, CA - - 2014
Cutaneous scarring is a major source of morbidity and current therapies to mitigate scar formation remain ineffective. Although wound fibrosis and inflammation are highly linked, only recently have mechanical forces been implicated in these pathways. Our group has developed a topical polymer device that significantly reduces post-injury scar formation via ...
Wu Chuanlong C Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedics, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, P.R. - - 2014
Osteoarthritis (OA) is one of the most widespread degenerative joint diseases affecting the elderly. Research into the regulatory mechanisms underlying the pathogenesis of OA is therefore warranted, and over the past decade, there has been an increased focus on the functional role of microRNAs (miRNAs or miRs). In this systematic ...
Kéri Szabolcs S Department of Cognitive Science, Budapest University of Technology and Economics, Budapest, Hungary; Nyírő Gyula Hospital - National Institute of Psychiatry and Addictions, Budapest, Hungary; Department of Physiology, Faculty of Medicine, University of Szeged, Szeged, Hungary. Electronic address: - - 2014
In recent years, increased attention has been paid to the inflammatory mechanisms of major depressive disorder (MDD). The aim of the present study was to investigate pro-inflammatory pathways related to the "leaky gut" hypothesis of MDD, which is based on the putative intestinal translocation of Gram-negative bacteria and a subsequent ...
Berkhout Ben B Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection & Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, The - - 2014
miRNAs are the key players of the RNAi mechanism, which regulates the expression of a large number of mRNAs in human cells. shRNAs are man-made synthetic miRNA mimics that exploit similar intracellular RNA processing routes. Massive amounts of data derived from next-generation sequencing have revealed miRNA species that are derived ...
Fontana R R Center of Excellence on Neurodegenerative Diseases and Department of Pharmacological and Biomolecular Sciences, University of Milan, - - 2014
Estrogens play an important role in the regulation of energy homeostasis in female mammals and a reduced ovarian function, due to natural aging or surgery, is associated with body weight increase and fat redistribution. This disruption of energy homeostasis may constitute a trigger for several pathologies known to be associated ...
Su Kang-Yi - - 2014
Pathogenesis of fibrosis is a common process leaded to chronic liver diseases and liver cirrhosis. New compounds for disease treatment and adjuvant therapy were an important issue in recent years. In this study, we isolated propolin G from Taiwanese green propolis (TGP) and investigated its antifibrotic effects by utilizing active ...
Monjaret François F Généthon, CNRS UMR8587, 1, rue de l'Internationale, 91000 Evry, - - 2014
Muscular dystrophies are a group of genetically distinct diseases for which no treatment exists. While gene transfer approach is being tested for several of these diseases, such strategies can be hampered when the size of the corresponding cDNA exceeds the packaging capacity of Adeno-Associated Virus vectors. This issue concerns, in ...
Isikbay Masis M Department of Medicine, Section of Hematology/Oncology, The University of Chicago, 5841 S. Maryland Ave. MC 2115, Chicago, IL, 60637, - - 2014
Despite new treatments for castrate-resistant prostate cancer (CRPC), the prognosis of patients with CRPC remains bleak due to acquired resistance to androgen receptor (AR)-directed therapy. The glucocorticoid receptor (GR) and AR share several transcriptional targets, including the anti-apoptotic genes serum and g lucocorticoid-regulated kinase 1 (SGK1) and Map kinase phosphatase ...
Kawasaki Yota Y Department of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medical Sciences, Kagoshima University, Kagoshima, - - 2014
The transcription factor NF-E2-related factor 2 (Nrf2) was originally identified to be a critical regulator of intracellular antioxidants and phase II detoxification enzymes. Recent studies have shown that high Nrf2 expression gives cancer cells an advantage for survival from anticancer chemotherapy and radiation therapy. The aims of this retrospective study ...
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