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Moshal Karni S KS Rhode Island Hospital, Alpert Medical School of Brown - - 2014
Background: We recently showed that progesterone treatment abolished arrhythmias and sudden cardiac death in a transgenic rabbit model of long QT syndrome type 2 (LQT2). Moreover, levels of cardiac sarcoplasmic reticulum Ca(2)(+) -ATPase2a (SERCA2a) were upregulated in LQT2 heart extracts. We hypothesized that progesterone treatment upregulated SERCA2a expression, thereby reducing ...
Saulnier Nathalie N Vetbiobank, Marcy l'Etoile, - - 2014
The anti-inflammatory and anti-catabolic effects of neonatal MSC were investigated in a xenogeneic model of mild osteoarthritis (OA). The paracrine properties of MSC on synoviocytes were further investigated in vitro. OA was induced by medial meniscal release (MMR) in 30 rabbit knees. A single early (day 3) or delayed (day ...
Li Yi - - 2014
Oocyte secreted factors (OSFs), including growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15), play an important role in the process of follicular development and oocyte maturation. Since OSFs are expressed in oocytes and cumulus granulosa cells, the aim of the present study was to explore whether the ...
Singhal Rahul R Division of Cardiology, Department of Medicine, Taipei Veterans General Hospital, Taipei, - - 2014
This study aimed to investigate the mechanism by which colchicine suppresses atrial fibrillation (AF) in a rabbit heart failure (HF) model. HF was induced by coronary ligation. Using the Langendorff perfusion system, monophasic action potentials were recorded in the left atrial appendage (LAA) of normal rabbits (n=6) and HF rabbits ...
Wallingford Mary C MC Department of Bioengineering, University of Washington, Seattle, WA, USA 91895. Electronic address: - - 2014
PiT-1 protein is a transmembrane sodium-dependent phosphate (Pi) transporter. PiT-1 knock out (KO) embryos die from largely unknown causes by embryonic day (E) 12.5. We tested the hypothesis that PiT-1 is required for endocytosis in the embryonic yolk sac (YS) visceral endoderm (VE). Here we present data supporting that PiT-1 ...
Gross-Thebing Theresa - - 2014
BackgroundWhole-mount in situ hybridization (WISH) is a fundamental tool used for studying the spatio-temporal expression pattern of RNA molecules in intact embryos and tissues. The available methodologies for detecting mRNAs in embryos rely on enzymatic activities and chemical reactions that generate diffusible products, which are not fixed to the detected ...
Morrow J L JL Hawkesbury Institute for the Environment, University of Western Sydney, Penrith, NSW, - - 2014
In tephritids, the sex-determination pathway follows the sex-specific splicing of transformer (tra) mRNA, and the cooperation of tra and transformer-2 (tra-2) to effect the sex-specific splicing of doublesex (dsx), the genetic double-switch responsible for male or female somatic development. The Dominant Male Determiner (M) is the primary signal that controls ...
Fabi Francois F F Fabi, Medical Biology, University of Quebec at Trois-Rivieres, Trois-Rivieres, - - 2014
The three isoforms of Akt, Akt1, Akt2 and Akt3, are crucial regulators of both normal and pathological cellular processes. Each of these isoforms exhibits a high level of homology and functional redundancy with each other. However, while being highly similar and structurally homologous, a rising amount of evidence is showing ...
Inamdar L S LS Molecular Endocrinology and Development Laboratory, Department of Zoology, Karnatak University, Dharwad 580 003, India.. Electronic address: - - 2014
Estrogen signalling is critical for ovarian differentiation in reptiles with temperature-dependent sex determination (TSD). To elucidate the involvement of estrogen in this process, adrenal-kidney-gonadal (AKG) expression of estrogen receptor (ERα) was studied at female-producing temperature (FPT) in the developing embryos of the lizard, Calotes versicolor which exhibits a distinct pattern ...
Yin Y Y College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, - - 2014
Crumbs homolog 3 (Crb3) is a member of the Crumbs family of proteins. This protein may play a role in epithelial cell polarity and is associated with tight junctions at the apical surface of epithelial cells. Alternative transcriptional splice variants that encode different Crb3 isoforms have been characterized. The expression ...
Jin Jiu-Zhen JZ Laboratory of vertebrate embryogenesis, Department of Molecular, Cellular & Craniofacial Biology and Birth Defects Center, University of Louisville, Louisville, KY, 40202, - - 2014
Background: Transforming growth factor-β3 (TGF-β3) plays a central role in mediating secondary palate fusion along the facial midline. However, the mechanisms by which TGF-β3 functions during secondary palate fusion are still poorly understood. Results: We found that mouse cytokeratin 6α and 17 mRNAs were expressed exclusively in the palate medial ...
Liu Han H Department of Veterinary Medicine, Agricultural College, Guangdong Ocean University, Zhanjiang, 524088, - - 2014
Dioxin-like PCB126 is a persistent organic pollutant that causes a range of syndromes including developmental toxicity. Dioxins have a high affinity for aryl hydrocarbon receptor (AhR) and induce cytochrome P4501A (CYP1A). However, the role of CYP1A activity in developmental toxicity is less clear. To better understand dioxin induced developmental toxicity, ...
Rayon Teresa T Stem Cell Biology Program, Department of Cardiovascular Development and Repair, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, - - 2014
The first lineage choice in mammalian embryogenesis is that between the trophectoderm, which gives rise to the trophoblast of the placenta, and the inner cell mass, from which is derived the embryo proper and the yolk sac. The establishment of these lineages is preceded by the inside-versus-outside positioning of cells ...
Peng M M Tongde Hospital of Zhejiang Province , Zhejiang - - 2014
The functions and mechanisms of the genomes from the different gametes on the epigenetic reprogramming of embryos are still unclear. In this study, the expression of enzymes typically associated with changes in epigenetic markers was measured in parthenogenetically-activated and in-vitro fertilised embryos. General control of nucleotide synthesis 5 (GCN5), histone ...
Guo Cui C A Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200135, People's Republic of - - 2014
SNAIL and SLUG are zinc-finger transcription factors that participate in the regulation of cell division, cell survival, mesoderm formation and epithelial-to-mesenchymal transition. We investigate the expression of SNAIL and SLUG during follicular maturation, ovulation and luteinization in the ovaries of both neonatal mice and gonadotropin-induced immature mice. Furthermore, we examine ...
Ohata Yoshihisa Y Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, - - 2014
Chemical reagent Ex-527 is widely used as a major inhibitor of Sirtuin enzymes, which are a family of highly conserved protein deacetylases and have been linked with caloric restriction and aging by modulating energy metabolism, genomic stability, and stress resistance. However, the extent to which Ex-527 controls early developmental events ...
Kumar Sandeep S Development, Aging, and Regeneration Program, Sanford-Burnham Medical Research Institute, La Jolla, CA 92037, - - 2014
Retinoic acid (RA) generated in the mesoderm of vertebrate embryos controls body axis extension by downregulating Fgf8 expression in cells exiting the caudal progenitor zone. RA activates transcription by binding to nuclear RA receptors (RARs) at RA response elements (RAREs), but it is unknown whether RA can directly repress transcription. ...
Gallina Donika D Department of Neuroscience, College of Medicine, The Ohio State University, 4190 Graves Hall, 333 West 10th Avenue, Columbus, OH 43210, - - 2014
Identification of the signaling pathways that influence the reprogramming of Müller glia into neurogenic retinal progenitors is key to harnessing the potential of these cells to regenerate the retina. Glucocorticoid receptor (GCR) signaling is commonly associated with anti-inflammatory responses and GCR agonists are widely used to treat inflammatory diseases of ...
Goljanek-Whysall Katarzyna K School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, - - 2014
Myogenesis involves the stable commitment of progenitor cells followed by the execution of myogenic differentiation, processes that are coordinated by myogenic regulatory factors, microRNAs and BAF chromatin remodeling complexes. BAF60a, BAF60b and BAF60c are structural subunits of the BAF complex that bind to the core ATPase Brg1 to provide functional ...
Mallol Anna A Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biociències, Universitat Autònoma de Barcelona , 08193, Bellaterra, Spain - - 2014
Abstract Faulty reprogramming of the donor somatic nucleus to a totipotent embryonic state by the recipient oocyte is a major obstacle for cloning success. Accordingly, treatment of cloned embryos with epigenetic modifiers, such as histone deacetylase inhibitors (HDACi), enhances cloning efficiency. The purpose of our study was to further explore ...
Singh K P KP Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, - - 2014
Parthenogenetically produced embryos and embryonic stem (ES) cells derived from them offer a unique model for investigating the role of transcription factors in embryonic genome activation (EGA), pluripotent lineage specification and in pluripotency and self-renewal of ES cells because of the unique nature of these embryos. There is little information ...
Landemaine Aurélie A INRA, UR1037 Fish Physiology and Genomics, F-35000 Rennes, - - 2014
Myomaker (also called Tmem8c), a new membrane activator of myocyte fusion was recently discovered in mice. Using whole mount in situ hybridization on zebrafish embryos at different stages of embryonic development, we show that myomaker is transiently expressed in fast myocytes forming the bulk of zebrafish myotome. Zebrafish embryos injected ...
Lee Joanne E JE Genetics Department, University of Melbourne, Royal Parade, Parkville, VIC, 3010, - - 2014
In Arabidopsis, SEUSS (SEU) and SEUSS-LIKE 2 (SLK2) are components of the LEUNIG (LUG) repressor complex that coordinates various aspects of post-embryonic development. The complex also plays a critical role during embryogenesis, as seu slk2 double mutants have small, narrow cotyledons and lack a shoot apical meristem (SAM). Here we ...
Kim Kee K KK Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD - - 2014
Rbfox3, a neuron-specific RNA-binding protein, plays an important role in neuronal differentiation during development. An isoform Rbfox3-d31, which excludes the 93-nucleotide cassette exon within the RNA recognition motif of chicken Rbfox3, has been previously identified. However, the cellular functions of Rbfox3-d31 remain largely unknown. Here we find that Rbfox3-d31 mRNA ...
Lee Kyung-Bon - - 2014
Transforming growth factor beta (TGFbeta) superfamily signaling controls various aspects of female fertility. However, the functional roles of TGFbeta-superfamily cognate signal transduction pathway components (e.g. SMAD2/3, SMAD4, SMAD1/5/8) in early embryonic development are not completely understood. We have previously demonstrated pronounced embryotropic actions of the TGFbeta superfamily member-binding protein, follistatin, ...
Gao Fei F F Gao, College of Veterinary Medicine, Jilin University, Changchun, - - 2014
The Wilms' tumor 1 gene (WT1), originally identified as a tumor suppressor associated with Wilms' tumors, encodes a zinc finger-containing transcription factor that is expressed in multiple tissues and is an important regulator of cellular and organ growth, proliferation, development, migration and survival. However, there is a deficiency of data ...
Lee Kyung-Bon - - 2014
We previously demonstrated embryotrophic actions of maternal (oocyte-derived) follistatin during bovine early embryogenesis. Classical actions of follistatin are attributed to inhibition of activity of growth factors including activins and bone morphogenetic proteins (BMP). However, temporal changes in BMP mRNA in early bovine embryos and the effects of exogenous BMP on ...
Vafaye Valleh Mehdi M Department of Animal Science, Faculty of Agriculture, University of Zabol, Zabol, Iran. Electronic address: - - 2014
Intrinsic defects within the embryos, reflected by elevated cell death and low proliferative ability, are considered the most critical factors associated with bovine infertility. The identification of embryonic factors, which are responsible for successful embryo development, is thus critical in designing strategies for infertility intervention. In this experiment, the possible ...
Liang Xiao-Huan XH South China Agricultural University, - - 2014
Embryo implantation is a highly synchronized process between an activated blastocyst and a receptive uterus. Successful implantation relies on the dynamic interplay of estrogen and progesterone, but the key mediators underlying embryo implantation are not fully understood. Here we show that transcription factor early growth response 1 (Egr1) is regulated ...
Fragoso Ana Catarina AC 1] Department of Pediatric Surgery, Hospital Universitario La Paz. Madrid, Spain [2] Laboratory of congenital malformations, INGEMM and IdiPaz Research Laboratory. Madrid, Spain [3] Faculty of Medicine, University of Porto. Porto, - - 2014
Background:Abnormal lung development was recently described in the rat model of esophageal atresia and tracheoesophageal fistula (EA-TEF). Since in this condition the ventral-to-dorsal switch of Shh expression in the foregut is disturbed, the present study tested the hypothesis that this abnormal expression at the emergence of the tracheo-bronchial bud might ...
Wei Yonglong Y State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, - - 2014
Nuclear receptor co-repressors (Ncor) are important for developmental and homeostatic processes in vertebrates, which exert transcriptional repression by coordinating with histone deacetylases (Hdac). However, little is known about their roles in definitive hematopoiesis. Here, we show that, in zebrafish, ncor2 is required for hematopoietic stem cell (HSC) development by repressing ...
Robertson Cayleih E CE Department of Integrative Biology, University of Guelph, Guelph, Ontario, - - 2014
In recent years, natural and anthropogenic factors have increased aquatic hypoxia the world over. In most organisms, the cellular response to hypoxia is mediated by the master regulator hypoxia-inducible factor-1 (HIF-1). HIF-1 also plays a critical role in the normal development of the cardiovascular system of vertebrates. We tested the ...
Gregor Thomas T Joseph Henry Laboratories of Physics, Princeton University, Princeton, NJ 085444, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA. Electronic address: - - 2014
Transcriptional regulation of gene expression is fundamental to most cellular processes, including determination of cellular fates. Quantitative studies of transcription in cultured cells have led to significant advances in identifying mechanisms underlying transcriptional control. Recent progress allowed implementation of these same quantitative methods in multicellular organisms to ask how transcriptional ...
Russo Roberta R Institute of Biomedicine and Molecular Immunology " A. Monroy", National Research Council, Palermo, - - 2014
Growing evidence suggests that the transcription factors belonging to the Jun family are involved in many important cellular events, such as the control of bone development in mammalians. We have characterized for the first time a member of the Jun family from embryos of the sea urchin Paracentrotus lividus. The ...
Bothma Jacques P JP Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720; - - 2014
We present the use of recently developed live imaging methods to examine the dynamic regulation of even-skipped (eve) stripe 2 expression in the precellular Drosophila embryo. Nascent transcripts were visualized via MS2 RNA stem loops. The eve stripe 2 transgene exhibits a highly dynamic pattern of de novo transcription, beginning ...
Schlosser Gerhard G Department of Zoology, School of Natural Sciences & Regenerative Medicine Institute (REMEDI), National University of Ireland, Galway, Galway, - - 2014
Cranial placodes contribute to many sensory organs and ganglia of the vertebrate head. The olfactory, otic, and lateral line placodes form the sensory receptor cells and neurons of the nose, ear, and lateral line system; the lens placode develops into the lens of the eye; epibranchial, profundal, and trigeminal placodes ...
Mo C C Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, PR - - 2014
This study characterized the glucagon-like peptide 2 receptor (GLP2R) gene of chickens because relatively little is known about the underlying mechanism of GLP2 actions in nonmammalian species. With the use of reverse transcription PCR, we first cloned the chicken GLP2R (cGLP2R) from adult intestine, which was predicted to encode a ...
Tian Yu-Mei YM Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai 201306, - - 2014
Insulin-like growth factor-binding protein 1 (IGFBP-1), a hypoxia-induced protein, is a member of the IGFBP family that regulates vertebrate growth and development. In this study, full-length IGFBP-1a cDNA was cloned from a hypoxia-sensitive Cyprinidae fish species, the blunt snout bream (Megalobrama amblycephala). IGFBP-1a was expressed in various organs of adult ...
Alexander Courtney C Department of Developmental and Cell Biology, University of California Irvine, Irvine, California, United States of - - 2014
Craniofacial development requires signals from epithelia to pattern skeletogenic neural crest (NC) cells, such as the subdivision of each pharyngeal arch into distinct dorsal (D) and ventral (V) elements. Wnt signaling has been implicated in many aspects of NC and craniofacial development, but its roles in D-V arch patterning remain ...
Zhang Yinfeng - - 2014
Data concerning effects of tetrabromobisphenol A (TBBPA) on thyroid hormone (TH)-dependent vertebrate development have been limited, although TBBPA has been demonstrated in vitro to disrupt the TH signaling pathway at the transcriptional level. In this study, we investigated the effects of TBBPA on T3-induced and spontaneous Xenopus laevis metamorphosis, which ...
Mace Jacob D JD The Edward Via College of Osteopathic Medicine, Blacksburg, VA, - - 2014
Exogenous administration of substance P (SP) exerts anorexigenic effects in both chicks and rats, but the central mechanism mediating this response is poorly understood. Therefore, this study was designed to elucidate mechanisms of SP-induced anorexia using chicks as models. Chicks that received intracerebroventricular (ICV) injections of SP dose-dependably reduced their ...
Zhao Haijing H State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR - - 2014
The present aimed to study if porcine circovirus type 2 (PCV2), which adhered to zona pellucida (ZP), was able to enter mature porcine oocytes with intact and damaged ZP. Four groups, including uninfected ZP-intact oocytes (UOZI), uninfected ZP-damaged oocytes (UOZD), PCV2-infected ZP-intact oocytes (POZI), and PCV2-infected ZP-damaged oocytes (POZD) were ...
Stoeckius Marlon M Systems Biology of Gene Regulatory Elements, Max Delbrück Center Berlin, Berlin, - - 2014
The oocyte-to-embryo transition (OET) is thought to be mainly driven by post-transcriptional gene regulation. However, expression of both RNAs and proteins during the OET has not been comprehensively assayed. Furthermore, specific molecular mechanisms that regulate gene expression during OET are largely unknown. Here, we quantify and analyze transcriptome-wide, expression of ...
Ji Qianqian Q State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou, P. R. - - 2014
OCT4 is a well-established regulator of pluripotency and nuclear reprogramming. To determine if improving OCT4 abundance can facilitate oocyte-mediated reprogramming in cloned porcine embryos, we artificially increased OCT4 levels by co-incubating donor cells with 50 ng/μL OCT4 plasmid. We observed higher rates of blastocyst formation (P<0.05) and lower levels of ...
Wakamatsu Yoshio Y Department of Developmental Neuroscience, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, Sendai, Miyagi, 980-8575, - - 2014
Compared to placental mammals, marsupials have short gestation period, and their neonates are relatively immature. Despite these features, marsupial neonates must travel from the birth canal to the teat, suckle and digest milk to complete development. Thus, certain organs and tissues of marsupial neonates, such as forelimbs to crawl and ...
Swope David D Department of Pathology and Laboratory Medicine, Rutgers-Robert Wood Johnson Medical School, 675 Hoes Lane Piscataway, NJ - - 2014
The embryonic heart tube is formed by the migration and subsequent midline convergence of two bilateral heart fields. In Drosophila the heart fields are organized into two rows of cardioblasts (CBs). While morphogenesis of the dorsal ectoderm, which lies directly above the Drosophila dorsal vessel (DV), has been extensively characterized, ...
Gou Wenyu W National Engineering Laboratory For Animal Breeding, China Agricultural University, Beijing 100193, - - 2014
Heme oxygenase 1 (HO-1), a rate-limiting enzyme of heme catabolism, has a crucial role of cytoprotective functions under hypoxia. The objective of the present study was to investigate potential differences in protective effect of HO-1 gene on chicken (Gallus gallus) embryo lung during late incubation. At embryonic day (D) 16, ...
Hribal Romy R Department Reproduction Biology, Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany. Electronic address: - - 2014
Oviductin is known to be a key player providing a convenient environment for the process of fertilization affecting this by direct interaction with oocytes and sperm. As in vitro embryo production in the context of assisted reproduction for endangered felids is still in the process of optimization, oviductin might be used ...
Zhou Naru N Anhui Provincial Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, - - 2014
Histone H3 lysine 27 acetylation (H3K27ac) is an active epigenetic modification which has been revealed to be associated with active gene expression. It was hypothesized that H3K27ac might also participate in the porcine somatic reprogramming process during early development of SCNT-derived embryos. The spatial and temporal expression profiles of H3K27ac ...
Laranjeiro Ricardo R Centre for Cell and Molecular Dynamics, Department of Cell and Developmental Biology, University College London, London WC1E 6DE, - - 2014
The circadian clock is known to regulate a wide range of physiological and cellular processes, yet remarkably little is known about its role during embryo development. Zebrafish offer a unique opportunity to explore this issue, not only because a great deal is known about key developmental events in this species, ...
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