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Younger Scott T ST Departments of Pharmacology and Biochemistry, UT Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390-9041, - - 2015
miRNAs are endogenous small RNAs that regulate gene expression through recognition of complementary RNA sequences. While miRNAs have generally been understood to repress gene expression posttranscriptionally through recognition of 3'-untranslated regions (3'-UTRs) of mRNA transcripts, they have the potential to target additional classes of RNAs. Understanding the expanding pool of ...
Papadopoulou Barbara B Research Center in Infectious Disease, CHU de Quebec Research Center, 2705 Laurier Blvd., Quebec, QC, Canada, G1V 4G2, - - 2015
Regulated mRNA turnover is a highly important process in the control of gene expression in Leishmania and related trypanosomatid protozoa, as these organisms lack control at the level of transcription initiation. A large number of Leishmania transcripts harbor in their 3'UTRs two phylogenetically distinct subfamilies of extinct Short Interspersed DEgenerate ...
Pritchett Christopher L CL Department of Microbiology University of Colorado School of Medicine, Aurora CO 80045 East Tennessee State University, Johnson City, - - 2014
Pseudomonas aeruginosa virulence components are subject to complex regulatory control primarily through two-component regulatory systems that allow for sensing and responding to environmental stimuli. In this study, the expression and regulation of the P. aeruginosa AlgZR two-component regulatory system were examined. Primer extension and S1 nuclease protection assays were used ...
Rossiter Amanda E - - 2014
Pet is a cytotoxic autotransporter protein secreted by the pathogenic Enteroaggregative Escherichia coli strain 042. Expression of Pet is co-dependent on two global transcription regulators: CRP (cyclic AMP receptor protein) and Fis (factor for inversion stimulation). At the pet promoter CRP binds to a single site centred at position ‑40.5 ...
Padmini Ekambaram E P.G. Department of Biochemistry, Bharathi Women's College, Affiliated to University of Madras, Chennai, 600108, Tamil Nadu, India, - - 2015
Induction of heme oxygenase-1 (HO-1) and extracellular signal-regulated kinase (ERK) is a crucial step in the cellular response to oxidative stress. Altered expression of such proteins in response to stress conditions is a key factor for the maintenance of cellular integrity and survival. Hypoxia-inducible factor-1 (HIF-1) and cytochrome P450 1A ...
Lee Thomas T Howard Hughes Medical Institute, University of Colorado, United - - 2014
MicroRNAs (miRNAs) modulate protein and mRNA expression through translational repression and/or mRNA decay. In this study, we combined SILAC-based proteomics and RNAseq to identify primary targets based on measurements of protein and mRNA repression and analysis of transcript 3'UTR sequences. The primary target set was used to compare different prediction ...
Finotti Alessia A Department of Life Sciences and Biotechnology, Section of Biochemistry and Molecular Biology, University of Ferrara, - - 2014
Rapamycin, an inhibitor of mTOR activity, is a potent inducer of erythroid differentiation and fetal hemoglobin production in β-thalassemic patients. Mithramycin (MTH) was studied to see if this inducer of K562 differentiation also operates through inhibition of mTOR. We can conclude from the study that the mTOR pathway is among ...
Kanjanaworakul Poonmanee P Center for Agricultural Biotechnology, Kasetsart University, Nakhon Pathom, 73140, - - 2014
We cloned and sequenced the myostatin (MSTN) gene of walking catfish and characterized its expression under different conditions. The full cDNA sequence of MSTN was 1,784 bp, containing an open reading frame of 1,191 bp, which encoded 396 amino acids. The deduced MSTN sequence contained functional sites similar to other members of ...
Sethi Isha - - 2014
The Transcription Factor (TF) p63 is a master regulator of epidermal development and differentiation as evident from the remarkable skin phenotype of p63 mouse knockouts. Furthermore, ectopic expression of p63 alone is sufficient to convert simple epithelium into stratified epithelial tissues in vivo and p63 is required for efficient transdifferentiation ...
Koster Maria J E MJ Department of Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, 3584 CG, Utrecht, The - - 2014
The activity and dynamic nature of TATA-binding protein (TBP) crucial to RNA polymerase II-mediated transcription is under control of the Mot1p and NC2 complexes. Here we show that both TBP regulatory factors play 'hidden' roles in ensuring transcription fidelity by restricting anti-sense non-coding RNA (ncRNA) synthesis. Production of anti-sense ncRNA ...
Schmidt Skye A SA Delaware Biotechnology Institute and Department of Plant and Soil Sciences, University of Delaware, Newark, DE 19711, - - 2014
In metazoans, cleavage by the endoribonuclease SMG6 is often the first degradative event in non-sense-mediated mRNA decay (NMD). However, the exact sites of SMG6 cleavage have yet to be determined for any endogenous targets, and most evidence as to the identity of SMG6 substrates is indirect. Here, we use Parallel ...
Weingarten-Gabbay Shira S Department of Computer Science and Applied Mathematics and the Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, - - 2014
A new study detects unstable nascent RNAs and uncovers thousands of transcription initiation sites in promoters and enhancers. Detailed analysis shows that these initiation sites have a similar architecture and that they are differentiated by post-transcriptional regulation rather than transcription initiation.
Zhao Xu X 1] Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Acaemy of Sciences, No. 1-7 Beichen West Road, Chaoyang District, Beijing 100101, China [2] University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, - - 2014
The role of Fat Mass and Obesity-associated protein (FTO) and its substrate N6-methyladenosine (m(6)A) in mRNA processing and adipogenesis remains largely unknown. We show that FTO expression and m(6)A levels are inversely correlated during adipogenesis. FTO depletion blocks differentiation and only catalytically active FTO restores adipogenesis. Transcriptome analyses in combination ...
Brunn Nicholas D - - 2014
Expression of human thymidylate synthase (hTS), a key enzyme in thymidine biosynthesis, is regulated on the translational level through a feedback mechanism that is rarely found in eukaryotes. At low substrate concentrations, the ligand-free enzyme binds to its own mRNA and stabilizes a hairpin structure that sequesters the start codon. ...
Rybak-Wolf Agnieszka A Laboratory for Systems Biology of Gene Regulatory Elements, Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, Robert-Rössle-Strasse 10, 13125 Berlin, - - 2014
The endoribonuclease Dicer is known for its central role in the biogenesis of eukaryotic small RNAs/microRNAs. Despite its importance, Dicer target transcripts have not been directly mapped. Here, we apply biochemical methods to human cells and C. elegans and identify thousands of Dicer-binding sites. We find known and hundreds of additional ...
Li Hai-Jun HJ Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Collaborative Innovation Center for Neurogenetics and Channelopathies, Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou, China; Key laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou Medical University, Guangzhou, - - 2014
Increased expression of sodium channel SCN3A, an embryonic-expressed gene, has been identified in epileptic tissues, which is believed to contribute to the development of epilepsy. However, the regulatory mechanism of SCN3A expression under epileptic condition is still unknown. Here we showed a high level of Scn3a mRNA expression in mouse ...
Suzuki Masatoshi M Department of Anesthesiology, Nihon University School of Dentistry at Matsudo, Chiba 271-8587, - - 2014
Differentiated embryo chondrocyte 2 (DEC2/Sharp-1/Bhlhe41), a basic helix-loop-helix (bHLH) transcription factor, has been shown to regulate the transcription of target genes by binding to their E-box elements. We identified a possible DEC2-response element (consensus E-box: CACGTG) in the promoter region of Twist1. Forced expression of DEC2 significantly repressed Twist1 promoter ...
Zi-Wei Liu L Department of Preventive Medicine, School of Environmental Science and Public Health, Wenzhou Medical University, University Town, Chashan, Wenzhou, Zhejiang 325035, - - 2014
Abstract Objective: To explore the relationship between birth weight and fat mass- and obesity-associated (FTO) gene expression and promoter methylation status in the Chinese population. Methods: Seventy-five neonates and their mothers were recruited from Yuying Children's Hospital of Wenzhou Medical University. Subjects were divided into three groups by birth weight: ...
Wang Ning N University of - - 2014
The swelling of the lodicule is responsible for floret opening in many grass species, allowing for pollen dispersal and cross-pollination. In barley, the closed floret habit (cleistogamy) is under the control of cly1, a gene which operates by inhibiting the development of the lodicule. In non-cleistogamous cultivars, cly1 mRNA is ...
Gong Xi X State Key Laboratory of Food Science and Technology, College of Life Sciences, Nanchang University, Nanchang, 330031, - - 2014
MicroRNAs (miRNAs) usually bind to their target mRNAs through imperfect base pairing in the 3'-untranslated regions (3' UTRs) and regulate target gene expression via post-transcriptional suppression. In recent years, computational approaches to predict miRNA targets have facilitated the identification of potential target sites. In this study, we used three programs ...
Dufresne Julie - - 2014
Pannexins (PANXS) are channel-forming proteins implicated in cellular communication through the secretion of biomolecules such as ATP and glutamate. PANX1 and PANX3 are expressed in the male rat reproductive tract and their levels are regulated by androgens in the epididymis. There is currently no information on the regulation of the ...
Liu Lian L Key Laboratory of Information Fusion Technology of Ministry of Education, School of Automation, Northwestern Polytechnical University, Xi'an, Shaanxi 710027, China. - - 2015
Biochemical modifications to mRNA, especially N6-methyladenosine (m(6)A) and 5-methylcytosine (m(5)C), have been recently shown to be associated with crucial biological functions. Despite the intriguing advancements, little is known so far about the dynamic landscape of RNA methylome across different cell types and how the epitranscriptome is regulated at the system ...
Lewis Michael W MW Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, - - 2014
Maize leaves have distinct tissues that serve specific purposes. The blade tilts back to photosynthesize and the sheath wraps around the stem to provide structural support and protect young leaves. At the junction between blade and sheath are the ligule and auricles, both of which are absent in the recessive ...
Chen Guodong G Jiangsu Key Laboratory for Translational Research and Therapeutics of Neuro-Psycho-Diseases, Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, PR China; Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, The First Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215123, PR - - 2014
The transcription factor c-MAF could be polyubiquitinated and subsequently degraded in the proteasomes. Theoretically, any lysine residues in c-MAF could be ubiquitinated. In the present study, we tried to find out the specific lysine residue(s) mediating c-MAF ubiquitination. Through a series of mutational screens from lysine (K) to arginine (R), ...
Schmidt H D HD Center for Neurobiology and Behavior, Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, - - 2014
Activation of AMPA receptors (AMPARs) in the nucleus accumbens is necessary for the reinstatement of cocaine-seeking behavior, an animal model of drug craving and relapse. AMPARs are tetrameric protein complexes that consist of GluA1-4 subunits, of which GluA2 imparts calcium permeability. Adenosine deaminase acting on RNA 2 (ADAR2) is a ...
Wang Qing - - 2014
BackgroundAssembled RNA Polymerase III complexes exert local effects on chromatin processes, including influencing transcription of neighboring RNA Polymerase II transcribed genes. These properties have been designated as ¿extra-transcriptional¿ effects of the Pol III complex. Previous coding sequence microarray studies using Pol III factor mutants to determine global effects of Pol ...
García Fernández R A RA Animal Medicine and Surgery Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, - - 2014
In Iberian pigs, a high conceptus loss occurs during the first 30 days of gestation. Although the exact causes for these losses have not been determined to date, the importance of blood vessel development during early pregnancy has been noted. The aim of this study was to analyze the messenger RNA ...
Lee Kuo-Ming KM Institute of Biomedical Sciences, Academia Sinica, Taipei, - - 2014
The spliceosomal factor TRAP150 is essential for pre-mRNA splicing in vivo and, when overexpressed, it enhances splicing efficiency. In this study, we found that TRAP150 interacted with the cleavage and polyadenylation specificity factor (CPSF) and co-fractionated with CPSF and RNA polymerase II. Moreover, TRAP150 preferentially associated with the U1 small ...
Seo Ji Hae JH SNU-Harvard Neurovascular Protection Research Center, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of - - 2014
ARD1 is an acetyltransferase with several variants derived from alternative splicing. Among ARD1 variants, mouse ARD1225 (mARD1225), mouse ARD1235 (mARD1235), and human ARD1235 (hARD1235) have been the most extensively characterized and are known to have different biological functions. In the present study, we demonstrated that mARD1225, mARD1235, and hARD1235 have ...
Jin Lin L a Division of Hematology and Oncology, Department of Medicine, UAB Comprehensive Cancer Center , University of Alabama at Birmingham , Birmingham , AL , - - 2014
Abstract We have previously reported the identification of a novel WD-domain protein, STRAP that plays a role in maintenance of mesenchymal morphology by regulating E-cadherin and that enhances tumorigenicity partly by downregulating CDK inhibitor p21(Cip1). However, the functional mechanism of regulation of E-cadherin and p21(Cip1) by STRAP is unknown. Here, ...
Estienne Anthony A INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37041 Tours, France; IFCE, F-37380 Nouzilly, - - 2014
In the ovary, anti-Müllerian hormone (AMH) is produced by the granulosa cells of growing follicles and can modulate the recruitment of primordial follicles and the follicle stimulating hormone (FSH)-dependent development of follicles. However, the regulation of its production remains poorly understood. Recently, a stimulating effect of the bone morphogenetic proteins ...
Ouyang Miao - - 2014
Menin regulates distinct cellular functions by regulating gene transcription through its interaction with partner transcription factors, but the exact mechanisms that control Menin levels remain largely unknown. Here we report that Men1 mRNA, encoding Menin, is a novel target of microRNA-29b (miR-29b) and that miR-29b/Men1 mRNA association regulates Menin expression ...
Jang Min-Kyung MK School of Korean Medicine, Pusan National University, #49 Busandae hak-ro, Mulguem-eup, Yangsan-si, Gyeongnam 609-735, South - - 2014
Activating transcription factor 3 (ATF3) is a stress-adaptive transcription factor that mediates cellular stress response signaling. We previously reported that ATF3 represses CCAAT/enhancer binding protein α (C/EBPα) expression and inhibits 3T3-L1 adipocyte differentiation. In this study, we explored potential role of ATF3 in negatively regulating peroxisome proliferator activated receptor-γ (PPARγ). ...
Han Minghai M Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake; Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, School of Life Sciences, Huaiyin Normal University, Huaian, 223300, China, - - 2014
N-glycosylation usually occurs at the Asn-Xaa-Ser/Thr sequon of glycoproteins in Pichia pastoris, exerting great effects on expression efficiency; however, Asn-Xaa-Thr is more efficiently glycosylated than Asn-Xaa-Ser. In this study, the role of the two sequons in the expression of recombinant elastase (rPAE) was investigated. At N43, N212, and N280 of ...
Antas Veronica I VI Disciplines of Physiology and Anatomy & Histology, School of Medical Sciences, Bosch Institute, University of Sydney, Camperdown, - - 2014
The yolk sac (YS) is a thin, bi-layered membrane encompassing the developing mammalian embryo in utero. The outer layer of the YS is composed of visceral endodermal cells derived from the primitive endoderm. The inner mesenchymal layer is highly vascularised and the first source of haematopoietic cells. YS haematopoiesis takes ...
Yan Jun J Beijing Institute of Basic Medical Sciences, 27 Taiping Road, Beijing, 100850, P. R. - - 2014
RNAIII is known as the key effector of staphylococcal accessory gene regulator (agr) quorum-sensing system, which plays a central role in the pathogenesis of Staphylococcus aureus. As a regulatory RNA, RNAIII regulates multiple targets, including exoproteins and cell-wall-associated proteins. Lipoteichoic acid synthase (LtaS) is involved in the synthesis of lipoteichoic ...
Qu Fufa F Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; Graduate School of the Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, - - 2014
The Akirin protein is a nuclear factor in the innate immune system that is highly conserved from insects to mammals and plays key roles in diverse biological processes, including immunity, myogenesis, development and the cellular stress response. However, the function of Akirins in mollusk, the second most diverse group of ...
He Tianyuan T Department of Dermatology, University of Michigan, Ann Arbor, MI, - - 2014
Transforming growth factor-β (TGF-β) is a major regulator of collagen gene expression in human skin fibroblasts. Cellular responses to TGF-β are mediated primarily through its cell surface type I (TβRI) and type II (TβRII) receptors. Ultraviolet (UV) irradiation impairs TGF-β signalling largely due to reduced TβRII gene expression, thereby decreasing ...
Sha Fushen F Zhongshan School of Medicine, Institute of Human Virology, Sun Yat-Sen University, Guangzhou 510080, China Key Laboratory of Tropical Disease Control, Sun Yat-Sen University, Ministry of Education, Guangzhou 510080, - - 2014
Nuclear factor-κB (NF-κB) is an important transcription factor. While the NF-κB signaling pathway is modulated by many microRNAs (miRNAs), very few have been reported to target NF-κB1 gene directly. In this study, we used multiple miRNA target prediction programs to predict miRNAs with putative NF-κB1 3'-untranslated region (UTR) binding sites. ...
Ogino Tomoaki T Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA - - 2014
The multifunctional RNA-dependent RNA polymerase L protein of vesicular stomatitis virus catalyzes unconventional pre-mRNA capping via the covalent enzyme-pRNA intermediate formation, which requires the histidine-arginine (HR) motif in the polyribonucleotidyltransferase domain. Here, the effects of cap-defective mutations in the HR motif on transcription were analyzed using an in vitro reconstituted ...
Thomason Maureen K MK Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institutes of Health, Bethesda, Maryland, - - 2014
While the model organism Escherichia coli has been the subject of intense study for decades, the full complement of its RNAs is only now being examined. Here we describe a survey of the E. coli transcriptome carried out using a differential RNA-seq approach, which can distinguish between primary and processed ...
Wen Duancheng D Ansary Stem Cell Institute and Department of Medicine, Weill Cornell Medical College, New York, NY, 10065, USA; Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medical College, New York, NY, 10065, - - 2014
Chromatin remodeling via incorporation of histone variants plays a key role in the regulation of embryonic development. The histone variant H3.3 has been associated with a number of early events including formation of the paternal pronucleus upon fertilization. The small number of amino acid differences between H3.3 and its canonical ...
Maruyama Yuka Y Department of Tissue Physiology, Tokyo University of Agriculture and - - 2014
Expression of brain-specific phenotypes increased in all trans retinoic acid (ATRA)-induced neural differentiation of mouse P19 embryonal carcinoma cells. Among these phenotypes, expression of class IVa β-tubulin isotype (TUBB4a) was particularly enhanced in neural differentiation. Transient transfection assays employing a reporter construct found that ATRA-mediated regulatory region of the TUBB4a ...
Kumari Sangeeta S Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi - 110067, - - 2014
Nuclear Receptor PXR is predominantly expressed in liver and intestine. Expression of PXR is observed to be dysregulated in various metabolic disorders indicating its involvement in disease development. However, information available on mechanisms of PXR self-regulation is fragmentary. The present investigation identifies some of the regulatory elements responsible for its ...
Caballero-Lima David - - 2014
Candida albicans hyphae grow in a highly polarised fashion from their tips. This polarised growth requires the continuous delivery of secretory vesicles to the tip region. Vesicle delivery depends on Sec2p, the Guanine Exchange Factor (GEF) for the Rab GTPase Sec4. GTP bound Sec4 is required for the transit of ...
Wang Jiawan J Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, - - 2014
The nuclear receptor complex for the insect steroid hormone, 20-hydroxyecdysone (20E), is a heterodimer of EcR and USP. It has been shown that Drosophila EcR and USP can be sumoylated in mammalian cells, but it is unknown whether EcR-USP sumoylation naturally occurs in Drosophila. In Drosophila cells, USP, but not ...
Ashwal-Fluss Reut R Biological Chemistry Department, Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, - - 2014
Circular RNAs (circRNAs) are widely expressed noncoding RNAs. However, their biogenesis and possible functions are poorly understood. Here, by studying circRNAs that we identified in neuronal tissues, we provide evidence that animal circRNAs are generated cotranscriptionally and that their production rate is mainly determined by intronic sequences. We demonstrate that ...
Cichon Ann-Christin AC Department of Biology and Biochemistry, University of Bath, Bath, - - 2014
Cellular expression of host prion protein (PrP) is essential to infection with prion disease. Understanding the mechanisms that regulate prion protein expression at both the transcriptional and translational levels is therefore an important goal. The cellular prion protein has been associated with resistance to oxidative, and its expression is also ...
Cho Yoon Mi YM Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of - - 2014
X-box binding protein 1 (XBP1), a transcription factor of the unfolded protein response, plays various roles in many biological processes. We examined the pro-adipogenic activity and its target genes during adipogenic differentiation in wild type and genetically-modified 3T3-L1 cells. Signalling pathways that contribute to Xbp1 mRNA splicing and the correlation ...
Juárez-Rodríguez Maria Dolores MD Research Group in Oral Health & Systemic Disease, University of Louisville School of Dentistry, - - 2014
The QseBC two component system plays a pivotal role in regulating virulence and biofilm growth of the oral pathogen Aggregatibacter actinomycetemcomitans. We previously showed that QseBC autoregulates the ygiW-qseBC operon. In this study, we characterized the promoter that drives ygiW-qseBC expression. Using lacZ transcriptional fusion constructs and 5'-rapid amplification of ...
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