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Li Zhipeng Z Department of Otorhinolaryngology Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. - - 2014
Interleukin (IL)-25, a cytokine of IL-17 family, can activate p38 Mitogen-Activated Protein kinases(MAPK) and Nuclear Factor(NF)-κB pathways to propagate Th2 responses. The allergic rhinitis mouse model was established by stimulating BALB/c mouse with ovalbumin (OVA). Then we detected expression of IL-25 and downstream p38MAPK and NF-κB. The expression of IL-25, ...
Li Yaqing - - 2014
BackgroundIn chronic obstructive pulmonary disease (COPD), two major pathological changes that occur are the loss of alveolar structure and airspace enlargement. To treat COPD, it is crucial to repair damaged lung tissue and regenerate the lost alveoli. Type II alveolar epithelial cells (AECII) play a vital role in maintaining lung ...
Gaspar-Ramírez Octavio O Laboratorio de Toxicología Molecular, Centro de Investigación Aplicada en Ambiente y Salud (CIAAS), Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luis Potosí , San Luis Potosí , México - - 2014
Abstract Recent studies have demonstrated that compounds inducing pro-inflammatory cytokines enhance AhR expression. The aim of this study was 2-fold: (1) to determine if two pro-inflammatory compounds, dichlorodiphenyldichloroethylene (DDE) and 2,2',4,4',5,5'-hexa-chlorobiphenyl (PCB 153), independently affect AhR gene expression in peripheral blood mononuclear cells (PBMC); and (2) if affected, to determine ...
Hou Xiaoxia X Department of Pulmonary Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, - - 2014
To investigate the expression pattern of histone deacetylase 9 in peripheral blood of patients with allergic asthma and its regulatory effect on the balance of Th17/Treg cells involved in the pathogenesis of asthma. FUGW vector was used to construct the Jurkat-HA-FOXP3 cell line. After histone deacetylase inhibitor-trichostatin A (TSA) treatment, ...
Qian J J Department of Emergency Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, - - 2014
Toll-like receptor 9 (TLR9), an important component of the innate immune system, contributes to the pathogenesis of lung injury. However, its role in paraquat (PQ)-induced lung injury has not been studied. Mice were divided into PQ group (n = 24) and control group (n = 12). Mice in both groups ...
Xie Min M Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; University of Pittsburgh Asthma Institute@UPMC, Pulmonary Allergy Critical Care Medicine Division, Department of Medicine, University of Pittsburgh, Pittsburgh, - - 2014
Severe asthma (SA) can involve both innate and type 2 cytokine-associated adaptive immunity. Although IL-27 has been reported to potentiate TH1 responses (including the chemokine CXCL9) and suppress TH2 responses, its function in asthmatic patients is unknown. We sought to evaluate IL-27 expression in human asthma alone and in combination ...
Patel Brijeshkumar S BS University of Sydney, Faculty of Pharmacy, Sydney, New South Wales, Australia ; - - 2014
β2-agonists are principally used in asthma to provide bronchodilation, however they also have anti-inflammatory properties due, in part, to their ability to upregulate mitogen-activated protein kinase phosphatase 1 (MKP-1) in a cAMP-dependent manner. Phosphodiesterases (PDEs) are attractive targets for potentiating the anti-inflammatory response. There are 11 subfamilies of PDE enzymes, ...
Hao Yonghua Y Department of Pathobiology, University of Illinois at Urbana-Champaign, - - 2014
Aberrant mucin secretion and accumulation in the airway lumen are clinical hallmarks associated with various lung diseases, such as asthma, chronic obstructive pulmonary disease (COPD) and cystic fibrosis (CF). Mycoplasma pneumoniae (MP), long appreciated as one of the triggers for acute exacerbations of chronic pulmonary diseases, has recently been reported ...
Shiono Osamu O Department of Otorhinolaryngology and Head and Neck Surgery, Yokohama City University School of Medicine, Yokohama, Japan; Department of Otorhinolaryngology, and Head and Neck Surgery, Yokohama City University Medical Center, Yokohama, Japan. Electronic address: - - 2014
Chronic rhinosinusitis (CRS) is thought to be a multifactorial disease, and it is classified into a number of subtypes according to clinicohistological features. Periostin, a 90-kDa secreted protein, was reported to exist in nasal polyps (NPs) associated with CRS. We compared the expression of periostin with the degree of eosinophilic ...
Zhang Zhaolin Z Northwestern University, Lurie Children's Research Center, Chicago, Illinois, United States ; - - 2014
Cystic fibrosis transmembrane conductance regulator gene (CFTR) expression in human airway epithelial cells involves the recruitment of distal cis-regulatory elements, which are associated with airway-selective DNase hypersensitive sites (DHS) at -44kb and -35kb from the gene. The -35kb site encompasses an enhancer that is regulated by the immune mediators Interferon ...
Melboucy-Belkhir Sara S INSERM UMR - - 2014
Aberrant expression of master phenotype regulators by lung fibroblasts may play a central role in idiopathic pulmonary fibrosis (IPF). Interrogating IPF fibroblast transcriptome datasets, we identified Forkhead Box F1 (FOXF1), a DNA-binding protein required for lung development, as a candidate actor in IPF. Thus, we determined FOXF1 expression levels in ...
Cao Qingsong Q Department of Otorhinolaryngology, The First Affiliated Hospital, Jinan University, Guangzhou, 510630, - - 2014
The expression and distribution of signal transducer and activator of transcription 3 (STAT3), phosphorylated STAT3 (p-STAT3), and vascular endothelial growth factor (VEGF), as well as eosinophil infiltration in nasal polyps was detected to examine their roles and correlations in the nasal polyp pathogenesis. Using the streptavidin-biotin-peroxidase (SP) method, immunohistochemistry was ...
Lin Hai H Department of Otorhinolaryngology, Eye and ENT Hospital of Fudan University, Shanghai, China; Department of Otorhinolaryngology, Fuzhou General Hospital, Fuzhou, Fujian, - - 2014
The pathogenesis of human chronic rhinosinusitis with nasal polyps (CRSwNP) comprising eosinophilic CRSwNP (ECRSwNP) and non-eosinophilic (nECRSwNP) is not completely understood. Recent evidence has suggested that platelet-derived growth factor receptor alpha (PDGFRα) is implicated in cell growth, transformation, proliferation, migration, and vascular permeability and platelet-derived growth factor-A (PDGF-A) is a ...
Lin Hai H Department of Otorhinolaryngology, Eye and ENT Hospital of Fudan University, Shanghai, China Department of Otorhinolaryngology, Fuzhou General Hospital, Fuzhou, Fujian, - - 2014
The pathogenesis of human chronic rhinosinusitis with nasal polyps (CRSwNP) remains undetermined. Livin is a member of the inhibitor of the apoptosis protein family proteins. Caspase-3 and second mitochondria-derived activator of caspases (Smac) are critical in the induction of apoptosis. However, little is known about their roles in CRSwNP. We ...
Cho See-Wun SW Department of Life and Nanopharmaceutical Science, Kyung Hee University, Hoegi Dong, Dongdaemun-Gu, Seoul, 130-701, - - 2014
The aryl hydrocarbon receptor (AHR) is responsible for susceptibility to its ligand-dependent responses. However, the effect of non-AHR factors is less clear. To explore the non-AHR factors, we used two mouse strains with different AHR genetic variants, namely C3H/lpr and MRL/lpr strains with Ala and Val as the 375th amino ...
Comer Brian S BS University of South - - 2014
MicroRNA(miR)-146a and miR-146b are negative regulators of inflammatory gene expression in lung fibroblasts, epithelial cells, monocytes, and endothelial cells. The abundance of cyclooxygenase-2 (COX-2) and IL-1β is negatively regulated by the miR-146 family, suggesting miR-146a and/or miR-146b might modulate inflammatory mediator expression in airway smooth muscle thereby contributing to pathogenesis ...
Boivin Roxane R Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, Quebec, - - 2014
Overexpression of the (+)insert smooth muscle myosin heavy chain (SMMHC) isoform could contribute to airway bronchospasm by increasing the velocity of contraction. Whether the (+)insert isoform is present in the small airways and its expression is reversible in asthma are unknown. To determine the anatomical location and the expression kinetics ...
François Déborah D 1] INSERM, U1148-LVTS, F-75018 Paris, France, Université Paris Diderot, Sorbonne Paris Cité, Paris, France [2] DHU FIRE, Paris, - - 2014
Idiopathic pulmonary fibrosis (IPF) is a chronic diffuse lung disease characterized by an accumulation of excess fibrous material in the lung. Protease nexin-1 (PN-1) is a tissue serpin produced by many cell types, including lung fibroblasts. PN-1 is capable of regulating proteases of both coagulation and fibrinolysis systems, by inhibiting, ...
Gardini Alessandro A Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA; The Wistar Institute, Philadelphia, PA 19104, - - 2014
In unicellular organisms, initiation is the rate-limiting step in transcription; in metazoan organisms, the transition from initiation to productive elongation is also important. Here, we show that the RNA polymerase II (RNAPII)-associated multiprotein complex, Integrator, plays a critical role in both initiation and the release of paused RNAPII at immediate ...
Homma Tetsuya T Northwestern University, Allergy and Immunology, Chicago, Illinois, United States, Showa University, Tokyo, Japan ; - - 2014
Rationale: Staphylococcus aureus (SA) colonization and infection is common and may promote allergic or inflammatory airway diseases such as asthma, cystic fibrosis, and chronic rhinosinusitis (CRS) by interacting with airway epithelial cells. Airway epithelial cells not only comprise a physical barrier, but also play key roles in immune, inflammatory, repair ...
Comer Brian S BS University of South Alabama, Dept. of Biochemistry and Molecular Biology, Mobile, Alabama, United States ; - - 2014
Cyclooxygenase-2 expression and PGE2 secretion from human airway smooth muscle cells (hASMCs) may contribute to β2-adrenoceptor hyporesponsiveness, a clinical feature observed in some patients with asthma. HASMCs from patients with asthma exhibit elevated expression of cytokine-responsive genes and in some instances this is attributable to an altered histone code and/or ...
Dileepan Mythili - - 2014
BackgroundThe cell-surface protein CD38 mediates airway smooth muscle (ASM) contractility by generating cyclic ADP-ribose, a calcium-mobilizing molecule. In human ASM cells, TNF-¿ augments CD38 expression transcriptionally by NF-¿B and AP-1 activation and involving MAPK and PI3K signaling. CD38 ¿/¿ mice develop attenuated airway hyperresponsiveness following allergen or cytokine challenge. The ...
Hecht Emelia E Department of Medicine, McGill University, Montreal, Quebec, - - 2014
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor implicated in the regulation of apoptosis and proliferation. Although activation of the AhR by xenobiotics such as dioxin inhibits the cell cycle and control apoptosis, paradoxically, AhR expression also promotes cell proliferation and survival independent of exogenous ligands. The microRNA ...
Giannetti M M Division of Allergy-Immunology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, - - 2014
Eosinophilic esophagitis (EoE) is a chronic inflammatory disease characterized pathologically by eosinophil infiltration. In addition to loss of barrier integrity, a dominant T Helper 2-associated immune response and strong allergic connection, the esophagus tissue undergoes dramatic changes, with frequent presence of mucosal rings, strictures, linear furrows, and trachealization. Although the ...
Graf Allyson A Central Texas Veteran's Health Care System/Scott & White - - 2014
Background: Histidine is converted to histamine by histidine decarboxylase (HDC). We have shown that cholangiocytes (i) express HDC; (ii) secrete histamine; and (iii) proliferate after histamine treatment via ERK1/2 signaling. In bile duct ligated (BDL) rodents, there is enhanced biliary hyperplasia, HDC expression and histamine secretion. Aim: to demonstrate that ...
Fonseca Linda L Texas Perinatal Group , Austin, TX , USA - - 2014
Abstract Objective: While prenatal administration of synthetic corticosteroids stimulates both fetal lung development and expression of pulmonary surfactant, the specific effects may depend on the corticosteroid formulation used. We compared the dose-dependent effects of various concentrations of two synthetic corticosteroids, betamethasone and dexamethasone, on steady state levels of surfactant protein ...
Herman Melissa A MA Neuroscience Research Center and NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, 10117 Berlin, - - 2014
The vesicular glutamate transporter (VGLUT) plays an essential role in synaptic transmission by filling vesicles with glutamate. At mammalian synapses, VGLUT expression level determines the amount of glutamate packaged into vesicles, and the specific paralog of VGLUT expressed affects the release probability. In this study, we investigate whether there is ...
Li Jian J Jinan Centre for Disease Control and Prevention, Jinan, Shandong 250001, P.R. - - 2014
β-catenin contributes to the pathogenesis of lung fibrosis. However, the expression of β-catenin in fibroblasts under fibrotic conditions has not been studied. We investigated the expression of β-catenin in lung fibroblasts from bleomycin (BLM)‑challenged mice and human lung fibroblasts treated with transforming growth factor β (TGF-β) or lysophosphatidic acid (LPA) ...
Luo Lei L Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, - - 2014
Corticotropin-releasing hormone (CRH), urotensin I (UI) and urotensin II (UII) are found throughout vertebrate species from fish to human. To further understand the role of crh, uI and uII in teleosts during development, we investigated the expression pattern of crh, uI, uIIα and uIIβ genes, and their response to acute ...
Hayashi Genki G Department of Molecular Biosciences, University of California, Davis, CA 95616, - - 2014
An inherited deficiency of the mitochondrial protein frataxin causes Friedreich's ataxia, the mechanism by which this deficiency triggers neuro- and cardio-degeneration is unclear. Microarrays of neural tissue of animal models of the disease showed decreases in antioxidant genes, and increases in inflammatory genes. Cyclooxygenase-derived oxylipins are important mediators of inflammation. ...
Masuda Keiko K Section of Behavioral Sciences, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima 739-8521, - - 2014
Neurotensin (NT) and neurotensin-related peptide (Lys(8), Asn(9), NT(8-13): LANT-6) have previously been purified from chicken intestine. However, the presence of these peptides and the localization of their precursor mRNA in the brain were not well understood. In the present study, through a comprehensive analysis of bioactive substances, NT and LANT-6 ...
Xie Ping P Key Laboratory of Soft Matter Physics and Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, China, - - 2014
The ribosome can translate through the duplex region or secondary structure of mRNA. Recent single-molecule experimental data showed that downstream mRNA secondary structures have more sensitive effects on deacylated tRNA dissociation from the E site than on tRNA translocation in the 50S subunit. However, it is unclear how the downstream ...
Hui Li L Department of Dermatology, Changhai Hospital, Second Military Medical University, Shanghai, - - 2014
Calcitriol had been proved to be effective for treatment of psoriasis vulgaris. However, the molecular events leading to the normalization of keratinocyte differentiation had not been fully explored. The aim of the study was to evaluate the role of calcitriol and serpin B1 in human keratinocyte cell line (HaCaT) proliferation. ...
Leem Yea-Hyun YH Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan-si, Chungnam, - - 2014
Magnesium metal and its alloys have been proposed as a novel class of bone implant biomaterials because of their biodegradability and mechanical properties. The purpose of this study was to determine whether magnesium ions, which are released abundantly from alloys, affect proliferation and differentiation of human bone marrow-derived stromal cells ...
Bakker A D AD Department of Oral Cell Biology, ACTA-University of Amsterdam and VU University Amsterdam, MOVE Research Institute Amsterdam, Amsterdam, The - - 2014
Mechanosensitive osteocytes regulate bone mass in adults. Interleukin 6 (IL-6), such as present during orthodontic tooth movement, also strongly affects bone mass, but little is known about the effect of IL-6 on osteocyte function. Therefore we aimed to determine in vitro whether IL-6 affects osteocyte mechanosensitivity, and osteocyte regulation of ...
Zheng Xiaowei X Department of Cell and Molecular Biology and Microbiology and Tumor Biology Center, Karolinska Institutet, SE-171 77 Stockholm, - - 2014
The cellular response to hypoxia is regulated by hypoxia-inducible factor-1α and -2α (HIF-1α and -2α). We have discovered that filamin A (FLNA), a large cytoskeletal actin-binding protein, physically interacts with HIF-1α and promotes tumor growth and angiogenesis. Hypoxia induces a calpain-dependent cleavage of FLNA to generate a naturally occurring C-terminal ...
He Wei W Consumer Science and Intelligent Systems, Food and Biobased Research, Wageningen University and Research Centre Wageningen, Netherlands ; Division of Human Nutrition, Sensory Science and Eating Behaviour, Wageningen University Wageningen, - - 2014
Why we like or dislike certain products may be better captured by physiological and behavioral measures of the autonomic nervous system (ANS) than by conscious or classical sensory tests. Responses to pleasant and unpleasant food odors presented in varying concentrations were assessed continuously using facial expressions and responses of the ...
Segall-Shapiro Thomas H TH Department of Biological Engineering, Synthetic Biology Center Massachusetts Institute of Technology, Cambridge, MA, - - 2014
Synthetic genetic systems share resources with the host, including machinery for transcription and translation. Phage RNA polymerases (RNAPs) decouple transcription from the host and generate high expression. However, they can exhibit toxicity and lack accessory proteins (σ factors and activators) that enable switching between different promoters and modulation of activity. ...
- - 2014
【Background】 Histamine (HA) and TGF-β, major mediators secreted by mast cells are involved in skin inflammation and systemic sclerosis. However, it remains largely unclear how the respective signaling are integrated to achieve development of skin fibrosis. 【Objectives】 Investigate the effect of HA signaling during myofibroblasts differentiation. 【Methods】 Skin fibroblasts (SFBs) ...
Shieh Jiunn-Min JM Department of Internal Medicine, Chi-Mei Medical Center, Tainan, Taiwan, - - 2014
The chemokine CXCL1 has been reported to be expressed in lung airway epithelium and non-small cell lung cancer biopsy specimens. In this study, we investigated the effects of TNF-α, an abundant cytokine detected in inflammation and various cancers, on CXCL1 release by human A549 lung carcinoma epithelial cells. CXCL1 expression ...
Moon Tae Chul TC Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, AB, - - 2014
PGD2 has long been implicated in allergic diseases. Recent cloning of a second PGD2 receptor, DP2 (also known as CRTh2), led to a greater understanding of the physiological and pathophysiological implications of PGD2. PGD2 signaling through DP1 and DP2 mediates different and often opposite effects in many cell types of ...
Shangguan Lingfei - - 2013
MicroRNAs (miRNAs) play an important role in post-transcriptional gene regulation that involved in various biological and metabolic processes. Many extensive studies have been done in model plant species, to discover miRNAs' regulating expression of their target genes and analyze their functions. But, the function of Poncirus trifoliata miRNAs has not ...
Zhang Wei - - 2013
ZBED6 was identified as a transcription factor that affects muscle mass and fat deposition in pigs. Mechanisms mediating effects on fat mass are unclear. The objective was to determine the effect of ZBED6 mRNA knockdown on 3T3-L1 preadipocyte differentiation and gene expression. Differentiation was associated with increased mRNA abundance of ...
Malott Rebecca J RJ Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada M5S - - 2013
Clinical and epidemiological synergy exists between the globally important sexually transmitted infections, gonorrhea and HIV. Neisseria gonorrhoeae, which causes gonorrhea, is particularly adept at driving HIV-1 expression, but the molecular determinants of this relationship remain undefined. N. gonorrhoeae liberates a soluble factor that potently induces expression from the HIV-1 LTR ...
Joshi P - - 2013
The past few years have seen significant advances in the use of modern proteomics approaches for biological discoveries. Among the fields impacted by proteomics is that of epigenetics, as mass spectrometry-based approaches have allowed the identification and characterization of transcriptional regulators, epigenetic marks, and the constantly evolving epigenetic landscape of ...
Wery Maxime - - 2013
Whole transcriptome RNA-Seq has emerged as a powerful tool in transcriptomics, enabling genome-wide quantitative analysis of gene expression and qualitative identification of novel coding or non-coding RNA species through transcriptome reassembly. Common protocols for preparation of RNA-Seq libraries include an RNA fragmentation step for which several RNA sizing techniques are ...
Katayama Kazuhiro - - 2013
P-glycoprotein (P-gp)/ABCB1 expression on cancer cell surfaces is a critical determinant of anticancer drug resistance. Regulators of P-gp expression and function are key molecules controlling drug resistance. Here we report the mechanism underlying the ubiquitin-proteasome pathway-mediated degradation of P-gp. The proteasome inhibitor MG132 increased P-gp level, enhanced its ubiquitination, and ...
Park June Hyun - - 2013
Caenorhabditiselegans microRNAs (miRNAs) bind to partially complementary sequences in the 3' untranslated region of target mRNAs, resulting in translational repression through mRNA destabilization. High-throughput sequencing of RNA cleavage fragments was performed to directly detect miRNA-directed cleavage targets in adult stage C. elegans. From this analysis, we found that miR-249 directed ...
- - 2013
AMPK loss induces aerobic glycolysis and promotes MYC-induced lymphomagenesis.
Wang Chang Rong - - 2013
Pholiota adiposa is a kind of edible mushroom which has long been known for its health care applications. To reveal the exact mechanism of its protective functions in humans, in this study we isolated and identified the active compound PB3 of P. adiposa for the first time by a combination ...
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