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Liu Xueqing - - 2014
LH regulation of the EGF network is critical for oocyte maturation and the ovulatory process. Recent studies have indicated that C-type natriuretic peptide (CNP) and its receptor natriuretic peptide receptor B (NPR2) play an important role in the control of meiotic arrest. Here, we investigated the involvement of the EGF ...
Foley Caitlin J CJ Molecular Oncology Research Institute, Tufts Medical Center, Tufts University School of Medicine, Boston, Massachusetts; Program in Genetics, Tufts University School of Medicine, Boston, - - 2014
Matrix metalloprotease-1 (MMP1) has been implicated in many human disease processes, however the lack of a well characterized murine homologue has significantly limited the study of MMP1 and the development of MMP-targeted therapeutics. The discovery of murine Mmp1a in 2001, the functional mouse homologue of MMP1, offers a valuable tool ...
Liu Hui H Department of Life Science, Hunan Agricultural University, Changsha, Hunan 410128, P.R. - - 2014
Tumor necrosis factor‑induced protein 1 (Tnfaip1), also known as B12, has been previously identified as a tumor necrosis factor-α (TNF-α)-inducible protein and is involved in the cytokinesis signaling pathway, DNA synthesis, innate immunity, cell apoptosis, Alzheimer's disease (AD) and type 2 diabetic nephropathy. However, little is known regarding the expression of Tnfaip1 ...
Okuda Hiroaki H Department of Anatomy and Neuroscience, Faculty of Medicine, Nara Medical - - 2014
OASIS, a basic leucine zipper transcription factor of the cAMP response element binding/Activating transcription factor family, is induced in reactive astrocytes in vivo and has important roles in quality control of protein synthesis at the endoplasmic reticulum. Reactive astrocytes produce a non-permissive environment for regenerating axons by up-regulating chondroitin sulfate ...
Guo Jia J Department of Nephrology, the First Affiliated Hospital of Zhengzhou University, Henan, - - 2014
Background: Epithelial-mesenchymal transition (EMT) is recognized to play an important role in diabetic nephropathy (DN). Objective: To analyze the roles of glycogen synthase kinase 3β (GSK-3β), β-catenin and Snail signaling in high glucose (HG)-induced mouse podocytes EMT. Methods: Differentiated podocytes were divided into: the normal glucose group (NG: glucose 5.6mM), ...
Yang Yurong Y State Key Laboratory of Cellular Stress Biology, School of Life Science, Xiamen University, Xiamen, Fujian, 361005, People's Republic of China, - - 2014
The Tsdaf-21 cDNA was cloned into pET-32a and subsequently expressed in Escherichia coli. The recombinant protein TsDAF-21 was purified and evaluated as an antigen in Western blot. The serum from mouse 14, 21, and 28 days after being infected with Trichinella spiralis recognized rTsDAF-21, but the serum from mouse 7 days post ...
Rowley Sean M SM University of Georgia, United - - 2014
In autoimmune diseases, accumulation of activated leukocytes correlates with inflammation and disease progression, and therefore, disruption of leukocyte trafficking is an active area of research. The serine-threonine protein kinase Tpl2 (MAP3K8) regulates leukocyte inflammatory responses and is also being investigated for therapeutic inhibition during autoimmunity. Herein, we addressed the contribution ...
Seifi Tahere T Department of Biology, School of Sciences, University of Isfahan, Isfahan, - - 2014
FNDC5 (also termed PEP) gene encodes a type I membrane protein which is cleaved and secreted as Irisin hormone. We have identified mouse putative core promoter of FNDC5 and characterized its activity. FNDC5 is located within mouse chromosome 4, spans about 7,534 bp, and consists of 6 exons. The mouse FNDC5 ...
Hanaki Ken-Ichi KI Department of Laboratory Animal Medicine; Center for In Vivo Sciences, Institute for Biomedical Sciences, Iwate Medical University, 2-1-1 Nishi-Tokuta, Yahaba, Iwate 028-3694, Japan. Electronic address: - - 2014
Murine norovirus (MNV) has considerable genetical and biological diversity and is recognized worldwide as the most common contaminant in laboratory mouse colonies. This study developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method with the potential to detect a broad range of MNV. RT-LAMP, using a set of 5 primers ...
Wang Chun-Yan CY China Medical University, Key Laboratory of Medical Cell Biology of Ministry of Education of China, Department of Pathophysiology, Shenyang, China, Northeastern University, College of Life and Health Sciences, Shenyang, China, China Medical University, Department of Nutrition and Food Hygiene, School of Public Health, Shenyang, China ; - - 2014
Aims: There is extensive evidence that oxidative stress induces cellular dysfunction in brain and plays a critical role in Alzheimer's disease (AD) pathogenesis. Hypoxia increases factors involved in oxidative stress injury and contributes to the onset and progression of AD. Nuclear factor erythroid 2-related factor 2 (Nrf2), a major component ...
Atmaca Hasan Tarık HT Department of Pathology, Kırıkkale University Faculty of Veterinary Medicine, Kırıkkale, Turkey. Electronic address: - - 2014
Toll-like receptor 11 (TLR11) is a specific receptor for Toxoplasma gondii and uropathogenic Escherichia coli and has recently been identified in the mouse brain. In the present study, TLR11 gene expression was measured in the mouse brain by reverse-transcription PCR (RT-PCR). Furthermore, the TLR11 protein expression profile was evaluated in ...
Luisier Raphaëlle R Preclinical Safety, Novartis Institutes for Biomedical Research, CH-4057 Basel, - - 2014
The constitutive androstane receptor (CAR) and the pregnane X receptor (PXR) are closely related nuclear receptors involved in drug metabolism and play important roles in the mechanism of phenobarbital (PB)-induced rodent nongenotoxic hepatocarcinogenesis. Here, we have used a humanized CAR/PXR mouse model to examine potential species differences in receptor-dependent mechanisms ...
Fischl Adrian M AM Department of Physiology, University of Kentucky, 800 Rose Street, Lexington, KY 40536-0298, USA - - 2014
Activity-dependent survival of olfactory sensory neurons (OSNs) may allow animals to tune their olfactory systems to match their odor environment. Activity-dependent genes should play important roles in this process, motivating experiments to identify them. Both unilateral naris occlusion of mice for 6 days and genetic silencing of OSNs decreased S100A5, ...
Kawane Tetsuya T Department of Cell Biology, Unit of Basic Medical Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, - - 2014
Runx2 is essential for osteoblast differentiation and chondrocyte maturation. The expression of Runx2 is the first requisite step for the lineage determination from mesenchymal stem cells to osteoblasts. Although the transcript from Runx2 distal promoter is majorly expressed in osteoblasts, the promoter failed to direct green fluorescent protein (GFP) expression ...
Wallington-Beddoe Craig T CT Westmead Institute for Cancer Research, Westmead Millennium Institute, University of - - 2014
Sphingosine kinase 2 (SK2) may have utility as a prognostic marker in inflammatory diseases such as cancer where it has been rationalized as candidate therapeutic target. Here we show that SK2 has an oncogenic role in acute lymphoblastic leukemia (ALL) by influencing expression of MYC. Genetic ablation of SK2 impaired ...
Buler Marcin M *Department of Pharmacology and Toxicology, Institute of Biomedicine, †Center for Cell-Matrix Research and ‡Biocenter Oulu, Department of Medical Biochemistry and Molecular Biology, and §Medical Research Center Oulu and ∥Institute of Clinical Medicine and Pediatrics, Clinical Research Center, Oulu University Hospital, University of Oulu, Oulu, - - 2014
The sirtuins (SIRTs; SIRT1-7) are a family of NAD(+)-dependent enzymes that dynamically regulate cellular physiology. Apart from SIRT1, the functions and regulatory mechanisms of the SIRTs are poorly defined. We explored regulation of the SIRT family by 2 energy metabolism-controlling factors: peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α) and AMP-activated ...
Zhang Huan H H Zhang, School of Life Sciences, University of Science and Technology of China, Hefei, - - 2014
In mammals, the primordial follicle pool, providing all oocytes available to a female throughout her reproductive life, is established perinatally. Dysregulation of primordial follicle assembly results in female reproductive diseases, such as premature ovarian insufficiency (POI) and infertility. Female mice lacking Dicer, a gene required for biogenesis of microRNAs, show ...
Li Yansen Y College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR - - 2014
The aim of the study was to investigate the response of the nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant system to elevated scrotal temperature in mouse testes. Eight-week-old mice were exposed to a single scrotal heat treatment (42°C for 25min). The testes displayed severe damage, with multinucleated giant cells, nuclear ...
Tekko Triin T Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 19 Ravila Street, 50411 Tartu, - - 2014
Wolframin (Wfs1) is a membrane glycoprotein that resides in the endoplasmic reticulum (ER) and regulates cellular Ca(2+) homeostasis. In pancreas Wfs1 attenuates unfolded protein response (UPR) and protects cells from apoptosis. Loss of Wfs1 function results in Wolfram syndrome (OMIM 222300) characterized by early-onset diabetes mellitus, progressive optic atrophy, diabetes ...
Chen Huan H Department of Biochemistry and Molecular Biology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, - - 2014
Liver regeneration after partial hepatectomy (PH) is a synchronized process that is precisely controlled by system-wide transcriptional regulatory networks. To clarify the transcriptional changes and regulatory networks that involve transcription factors (TFs) and their target genes during the priming phase, an advanced mouse oligonucleotide array-based transcription factor assay (MOUSE OATFA), ...
Li Yushan - - 2014
Abnormalities in pyloric development or in contractile function of the pylorus cause reflux of duodenal contents into the stomach and increase the risk of gastric metaplasia and cancer. Abnormalities of the pyloric region are also linked to congenital defects such as the relatively common neonatal hypertrophic pyloric stenosis, and primary ...
Liu Sheng - - 2014
The formation of discrete elastin bands at the tips of secondary alveolar septa is important for normal alveolar development, but the mechanisms regulating the lung elastogenic program are incompletely understood. JNK suppress elastin synthesis in the aorta and is important in a host of developmental processes. We sought to determine ...
Yuan Yunsheng Y Shanghai Key Laboratory of Veterinary Biotechnology, College of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Rd, 200240, Shanghai, People's Republic of China, - - 2014
Secreted phosphoprotein 1 (SPP1) is a phosphorylated acidic glycoprotein. It is broadly expressed in a variety of tissues, and it is involved in a number of physiological and pathological events, including cancer metastasis, tissues remodeling, pro-inflammation regulation, and cell survival. SPP1 has shown its function of protecting tissues and organs ...
Suragani Rajasekhar N V S RN Acceleron Pharma, Cambridge, Massachusetts, - - 2014
Erythropoietin (EPO) stimulates proliferation of early-stage erythrocyte precursors and is widely used for the treatment of chronic anemia. However, several types of EPO-resistant anemia are characterized by defects in late-stage erythropoiesis, which is EPO independent. Here we investigated regulation of erythropoiesis using a ligand-trapping fusion protein (ACE-536) containing the extracellular ...
Bélanger Jasmin Stéphanie S Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada, H4A 2B4; Douglas Mental Health University Institute, 6875 LaSalle Blvd, Verdun, Quebec, Canada, H4H - - 2014
Entorhinal cortex lesioning (ECL) causes an extensive deafferentation of the hippocampus that is classically followed by a compensatory reinnervation, where apolipoprotein E, the main extracellular lipid-carrier in the CNS, has been shown to play a crucial role by shuttling cholesterol to reconstructing neurons terminals. Hence, we investigated whether the ATP-binding ...
You Linya L The Rosalind & Morris Goodman Cancer Research Center; Montreal, QC Canada; Department of Medicine; McGill University; Montreal, QC - - 2014
Bromodomain- and PHD finger-containing protein 1 (BRPF1) is a unique epigenetic regulator that contains multiple structural domains for recognizing different chromatin modifications. In addition, it possesses sequence motifs for forming multiple complexes with three different histone acetyltransferases, MOZ, MORF, and HBO1. Within these complexes, BRPF1 serves as a scaffold for ...
Bhardwaj A A Department of Oncologic Sciences, Mitchell Cancer Institute, University of South Alabama, Mobile, AL 36604, - - 2014
Background:Emergence of castration-resistance in prostate cancer (PCa) is invariably associated with aggressive and metastatic disease. Previously, we reported promotion of castration-resistance upon downregulation of PPP2CA (encoding catalytic subunit of protein phosphatase 2A (PP2A), α-isoform); however, its role in PCa growth and metastasis remained undetermined.Methods:PPP2CA was overexpressed/silenced in PCa cells by ...
Sun Guang-Yong GY Capital Medical University, Cell Biology, Beijing, China ; - - 2014
HSS (hepatic stimulator substance), also known as ALR (augmenter of liver regeneration), acts as a hepatotrophic growth factor to promote liver regeneration after liver damage or partial hepatectomy. However, the expression and function of HSS during liver development in mammals remain largely unknown. In this work, the hepatoblasts were isolated ...
Philips Mari-Anne MA Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, 50411, Estonia, - - 2014
Limbic system-associated membrane protein (LSAMP) is a neural cell adhesion molecule involved in neurite formation and outgrowth. The purpose of the present study was to characterize the distribution of alternatively transcribed Lsamp isoforms in the mouse brain and its implications on the regulation of behavior. Limbic system-associated membrane protein 1b ...
Pant Vinod V Department of Genetics, M.D. Anderson Cancer Center, Houston, - - 2014
The p53-Mdm2 feedback loop is thought to be the main mechanism by which p53 autoregulates its levels and activity after DNA damage. We tested this paradigm in a genetically engineered mouse model in which the feedback loop was disrupted by point mutations in the p53 binding site of the Mdm2 ...
Liu Jing J Institute For Medical Biology and Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan, 430074, - - 2014
MTMR14 is a phosphoinositide phosphatase, which has been reported to regulate the maintenance of normal muscle performance and aging in mice. However, the function of MTMR14 in mouse embryonic fibroblasts (MEFs) remains largely unknown. In this study, we established MTMR14 WT and KO MEFs and showed that MTMR14 is localized ...
Uekusa Shota S Department of Pediatric Surgery, School of Medicine, Nihon University, Tokyo, - - 2014
Aberrant methylation of Nr4a3 exon 3 CpG island (CpGi) was initially identified during multistep mouse skin carcinogenesis. Nr4a3 is also known as a critical gene for neuronal development. Thus, we examined the Nr4a3 exon 3 CpGi methylation in mouse brain tissues from 15-day embryos, newborns and 12-week-old adults and found significant ...
Jiang Zhihua Z Boston University School of Medicine, Medicine, Boston, Massachusetts, United States ; - - 2014
Shh is expressed and secreted from the embryonic lung epithelium and acts on the adjacent mesenchymal cells via its receptor Patched (Ptch)/Smoothened (Smo) and transcriptional effectors Gli proteins. Genetic studies showed that the Sonic hedgehog (Shh) pathway plays critical roles in mouse lung development. However, little is known about miRNAs ...
Jambalganiin Ulziisaikhan U Department of Microbiology and Immunology, Aichi Medical University School of Medicine, Nagakute, Aichi 480-1195, - - 2014
The inhibitory effect of valproic acid (VPA) on lipopolysaccharide (LPS)-induced inflammatory response was studied by using mouse RAW 264.7 macrophage-like cells. VPA pretreatment attenuated LPS-induced phosphorylation of phosphatidylinositol 3-kinase (PI3K) and Akt, but not nuclear factor (NF)-κB and mitogen-activated protein kinases. VPA reduced phosphorylation of MDM2, an ubiquitin ligase and ...
Xu Xiu X College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; School of Life Science, Northeast Agricultural University, Harbin 150030, - - 2014
Glutathione peroxidase 3 (GPX3) is an important member of antioxidant enzymes for reducing reactive oxygen species and maintaining the oxygen balance. Gpx3 mRNA is strongly expressed in decidual cells from days 5 to 8 of pregnancy. After pregnant mice are treated with GPX inhibitor for 3days, pregnancy rate is significantly ...
Kim Jung Ha JH Department of Pharmacology, Medical Research Center for Gene Regulation and BK21 plus, Chonnam National University Medical School, Gwangju 501-746, - - 2014
Osteoblasts not only control bone formation but also support osteoclast differentiation. Here we show the involvement of Kruppel-like factor 4 (KLF4) in the differentiation of osteoclasts and osteoblasts. KLF4 was down-regulated by 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) in osteoblasts. Overexpression of KLF4 in osteoblasts attenuated 1,25(OH)2D3-induced osteoclast differentiation in co-culture of mouse ...
Zhao Xiaoping X Department of Medicine, Division of - - 2014
Dysregulation of lipid homeostasis is intimately associated with obesity, type 2 diabetes and cardiovascular diseases. Sterol regulatory-element binding proteins (SREBPs) are the master regulators of lipid biosynthesis. Previous studies have shown that the conserved transcriptional cofactor Mediator complex is critically required for the SREBP transcriptional activity and recruitment of the ...
Verma M M Immunobiology Division, CSIR-Indian Institute of Toxicology Research, Lucknow, 226001, India, - - 2014
DNMT1 is the most important methyltransferase enzyme, involved in the regulation of gene expression and appropriate histone modification. It interact with proliferating cell nuclear antigen (PCNA), SNF2 family member ATP-dependent chromatin remodeling enzyme, cyclin dependent kinases inhibitor, E2F1 transcription factor and HDACs to form a repressor complex known as HDAC ...
Witsch Thilo J TJ University of Giessen and Marburg Lung Center, - - 2014
Aberrant remodelling of the extracellular matrix in the developing lung may underlie arrested alveolarisation associated with bronchopulmonary dysplasia (BPD). Transglutaminases are regulators of extracellular matrix remodelling. Therefore, the expression and activity of transglutaminases were assessed in lungs from human neonates with BPD and in a rodent model of BPD.Transglutaminase expression ...
Auret Jennifer J Neurology Research Group, Department of Medicine, University of Cape Town, Cape Town, South Africa; Cell Biology, Department of Human Biology, University of Cape Town, Cape Town, South - - 2014
Decay accelerating factor (DAF) expression at the muscle endplate is an important defence against complement-mediated damage in myasthenia gravis. Previously we implicated the c.-198C>G DAF polymorphism with the development of treatment-resistant myasthenia-associated ophthalmoplegia by showing that the C>G DAF polymorphism prevented lipopolysaccharide-induced upregulation of lymphoblast DAF. We postulated that drugs ...
Xu Xu X Weill Cornell Medical College, United - - 2014
CREBH is an endoplasmic reticulum (ER) anchored transcription factor that is highly expressed in the liver and small intestine and implicated in nutrient metabolism and proinflammatory response. Apolipoprotein A-IV (apoA-IV) is a glycoprotein secreted primarily by the intestine and to a lesser degree by the liver. ApoA-IV expression is suppressed ...
Das Laxmidhar L Biochemistry and Molecular Biology Laboratory, Department of Zoology, Banaras Hindu University, Varanasi221005, India. Electronic address: - - 2014
Interleukin-1 (IL-1α and IL-1β) is a prototypic, potent, multifunctional proinflammatory cytokine affecting almost all cell types. Expression of IL-1 is up regulated in different tumor phenotypes and is implicated as an important factor in tumor progression via expression of metastatic, angiogenic genes and growth factors. Therefore, down regulation of expression ...
Sebti Salwa S Institut de Recherche en Cancérologie de Montpellier, F-34298 Montpellier, - - 2014
Autophagy is regulated by posttranslational modifications, including acetylation. Here we show that HLA-B-associated transcript 3 (BAT3) is essential for basal and starvation-induced autophagy in embryonic day 18.5 BAT3(-/-) mouse embryos and in mouse embryonic fibroblasts (MEFs) through the modulation of p300-dependent acetylation of p53 and ATG7. Specifically, BAT3 increases p53 ...
Stoya Gudrun G Institute of Anatomy, Friedrich Schiller University School of Medicine, Jena University Hospital, 07743, Jena, - - 2014
Cadherins are calcium-depending cell adhesion proteins that play critical roles in brain morphogenesis and wiring. They provide an adhesive code for the development of cortical layers, due to their homophilic interactions and their restricted spatiotemporal expression patterns. In the adult organism, cadherins are involved in the maintenance and plasticity of ...
Kawashima Rie R Department of Environmental Pathology and Research Institute of Salivary Gland Health Medicine, Kanagawa Dental University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan. - - 2014
Purpose: Gastrointestinal integrity and immune surveillance are affected by stress. Stress also adversely affects mucosal barrier function. β-defensins constitute an integral component of the innate immune system as antimicrobial peptides, serving as the first line of defense against microbial pathogens at the epithelial surfaces of the upper digestive mucosa. The ...
Boucher Eric - - 2014
Glucocorticoids exert recognized positive effects on lung development. The genes involved in the classical pathway of glucocorticoid synthesis normally occurring in adrenals were found to be expressed on gestation day (GD) 15.5 in the developing mouse lung. Recently, expression of two of these genes was also detected on GD 17.5 ...
Mo Ji-Hun JH Department of Otorhinolaryngology, Dankook University College of Medicine, Cheonan, South - - 2014
Hypoxia-inducible factor (HIF) 1alpha is expressed under hypoxic conditions and plays an important role in immune and inflammatory responses. The role of HIF-1alpha in allergic airways has been investigated mainly in bronchial asthma. This study investigated the role of HIF-1alpha in mouse models and patients with allergic rhinitis (AR). Balb/c ...
Shimizu Natsumi N Laboratory of Molecular Developmental Biology, Graduate School of Biology-Oriented Science and Technology, Kinki University, Wakayama 649-6493, - - 2014
In the mammalian testis, the ubiquitin-proteasome system plays important roles in the process that promotes the formation of mature sperm. We recently identified zygote-specific proteasome assembly chaperone (ZPAC), which is specifically expressed in the mouse gonads and zygote. ZPAC mediates a unique proteasome assembly pathway in the zygote, but the ...
Choi H H 1] Department of Biochemistry and Molecular Biology, Integrated Genomic Research Center for Metabolic Regulation, Institute of Genetic Science, Yonsei University College of Medicine, Seoul, Korea [2] Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, - - 2014
Mouse 3T3-L1 preadipocytes differentiate into adipocytes when treated with 3-isobutyl-1-methylxanthine, dexamethasone, and insulin. Although mechanisms of adipogenesis, including transcriptional cascades, are understood, it is still unclear how clonally expanded cells eventually enter the terminal differentiation program. From gene expression profile studies, we identified G0/G1 switch gene 2 (G0s2) as a ...
Kim Soon-Ja SJ Biomedical Science, Department of Medicine, Chung-Ang University Graduate School, Seoul, - - 2014
We established an orthotopic non-muscle invasive bladder cancer (NMIBC) mouse model expressing the mammalian target of the rapamycin (mTOR) signaling pathway. After intravesical instillation of KU-7-lucs (day 0), animals were subsequently monitored by bioluminescence imaging (BLI) on days 4, 7, 14, and 21, and performed histopathological examination. We also validated ...
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