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Panigrahy Dipak D 1Vascular Biology Program, Boston Children's Hospital 2Department of Surgery, Harvard Medical School 3Center for Vascular Biology Research, Beth Israel Deaconess Medical Center, Boston, MA, United States 4Department of Surgery, Klinikum Grosshadern, LMU Munich, Munich, Germany 5Department of Ophthalmology, Harvard Medical School 6Department of Pathology, Harvard Medical School 7Schepens Eye Research Institute, Massachusetts Eye & Ear, Boston, MA, United - - 2014
Neuropilin-1 (NRP1) is a receptor for vascular endothelial growth factor (VEGF). A soluble isoform of Nrp1 (sNrp1) has not been described in the mouse. Our goal was to examine the expression of mouse sNrp1 during liver development and regeneration.sNrp1 was cloned from mouse liver. The expression of sNrp1 and VEGF ...
Reeh Kaitlin A G KA Department of Molecular Carcinogenesis, University of Texas, M.D. Anderson Cancer Center, Science Park Research Division, Smithville, TX 78957, - - 2014
The thymus and parathyroid glands arise from a shared endodermal primordium in the third pharyngeal pouch (3rd pp). Thymus fate is specified in the ventral 3rd pp between E9.5 and E11, whereas parathyroid fate is specified in the dorsal domain. The molecular mechanisms that specify fate and regulate thymus and ...
Tanaka Yuka Y Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan Center for Clinical and Translational Research, Kyushu University Hospital, Fukuoka, - - 2014
Docking protein 2 (Dok2) is an adapter protein which is involved in hematopoiesis. However, it still remains unclear how Dok2 functions in regulation of transcription of hematopoietic genes. To address this issue, we knocked-down Dok2 mRNA in mouse erythroleukemia cells which highly express Dok2 intrinsically. Mouse erythroleukemia cells were transfected ...
Jin Chengri C Department of Orthodontics, Yonsei University College of Dentistry, 50 Yonsei-Ro,, Seodaemun-Gu,, Seoul,, 120-752, - - 2014
Mammalian palate development is regulated by complex processes. Many cellular and molecular events, such as cell proliferation, apoptosis, cell migration and the epithelial mesenchymal transition, regulate proper palate development and some abnormalities in palate development lead to cleft palate. Various developmental disorders, such as cleft palate and disorders of the ...
Polito Vinicia A VA Huffington Center on Aging, Baylor College of Medicine, Houston, TX, - - 2014
Accumulating evidence implicates impairment of the autophagy-lysosome pathway in Alzheimer's disease (AD). Recently discovered, transcription factor EB (TFEB) is a molecule shown to play central roles in cellular degradative processes. Here we investigate the role of TFEB in AD mouse models. In this study, we demonstrate that TFEB effectively reduces ...
Zhao Xiaoyun X Clinical Laboratory, Hebei General Hospital, Shijiazhuang, Hebei 050057, P.R. - - 2014
MicroRNAs (miRNAs) are a class of non‑coding 18‑25-nucleotide endogenous RNA molecules. miRNAs act as specific gene silencers to regulate target gene expression at the posttranscriptional level, by base pairing to the 3'‑untranslated region of the target mRNA. miR‑152 is an miRNA that was originally identified in cancer cells, and was ...
Zhou Yan Y Department of Neurobiology, Mount Sinai School of Medicine, New York, NY 10029. Electronic address: - - 2014
In humans, alcoholism is associated with a decrease in basal ACTH and cortisol levels, and blunted pituitary ACTH responses to administered corticotropin-releasing hormone (CRH) during active drinking and after long-term abstinence. Preclinical studies indicate that a persistent decrease in pituitary activation after chronic exposure to ethanol is due to a ...
Rentsendorj Otgonchimeg O Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins Medical Institutions, Baltimore, Maryland, - - 2014
PDE2A is a dual-function PDE that is stimulated by cGMP to hydrolyze cAMP preferentially. In a two-hit model of ALI, we found previously that PDE2A decreased lung cAMP, up-regulated lung iNOS, and exacerbated ALI. Recent data suggest that macrophage iNOS expression contributes to ALI but later, promotes lung-injury resolution. However, ...
Zhang Deqiang D University of Michigan Medical School, United - - 2014
The clock protein BMAL1 participates in circadian regulation of lipid metabolism, but its contribution to insulin-AKT-regulated hepatic lipid synthesis is unclear. Here we used both Bmal1-/- and acute liver-specific Bmal1-depleted mice to study the role of BMAL1 in refeeding-induced de novo lipogenesis in the liver. Both global deficiency and acute ...
Shiizaki Kazuhiro K National Cancer Center Research - - 2014
Omeprazole (OME), a proton pump inhibitor used to treat gastritis, is also an aryl hydrocarbon receptor (AhR) activator. OME activates AhR in human hepatocytes and hepatoma cells but not in mouse in vivo or in vitro. We recently discovered that this species-specific difference results from a difference in a few ...
Zhu Hong-Y HY *Singapore Eye Research Institute, Singapore, Singapore; and †SRP Neuroscience and Behavioral Disorders, Duke-NUS, Singapore, - - 2014
The aim of this study was to compare the expression patterns of 3 important biochemical characteristics of fibrosis-moesin, transforming growth factor (TGF)-β1, and α-smooth muscle actin (SMA) in the mouse cornea with fibrosis induced by common etiologies-sterile mechanical injury and infection. Corneas of 8-week-old C57BL6 mice were either wounded after ...
Best Sarah A SA ACRF Stem Cells and Cancer Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia Department of Medical Biology, The University of Melbourne, Parkville, Victoria 3010, - - 2014
The HLH transcriptional regulator Id4 exerts important roles in different organs, including the neural compartment, where Id4 loss usually results in early lethality. To explore the role of this basally restricted transcription factor in the mammary gland, we generated a cre-inducible mouse model. MMTV- or K14-cre-mediated deletion of Id4 led ...
Mao Hui Z HZ Cedars-Sinai Medical Center, United - - 2014
Lipase maturation factor 1 (Lmf1) is a critical determinant of plasma lipid metabolism, as demonstrated by severe hypertriglyceridemia associated with its mutations in mice and human subjects. Lmf1 is a chaperone localized to the ER and required for the post-translational maturation and activation of several vascular lipases. Despite its importance ...
Garcia Virginia B VB Department of Biological Sciences, University of Missouri, Columbia, MO USA - - 2014
Neural networks ultimately arrive at functional output via interaction of the excitability of individual neurons and their synaptic interactions. We investigated the relationships between voltage-gated ion channel and neurotransmitter receptor mRNA levels in mouse spinal cord at four different postnatal time points (P5, P11, P17, and adult) and three different ...
Szafranski Karol K Fritz Lipmann Institute-Institute for Age Research, Beutenbergstr. 11, 07745 Jena, Germany - - 2014
Thousands of tandem alternative splice sites (TASS) give rise to mRNA insertion/deletion variants with small size differences. Recent work has concentrated on the question of biological relevance in general, and the physiological regulation of TASS in particular. We have quantitatively studied 11 representative TASS cases in comparison to one mutually ...
Xu Yuanhong Y Department of Pancreatic Gastroenterologic Surgery, First Hospital of China Medical University, No. 92, Nanjing Rd, Shenyang, 110001, China. Electronic address: - - 2014
Jun dimerization protein 2 (JDP2) is a repressor of transcription factor AP-1. To investigate the transcriptional regulation of the JDP2 gene, we cloned the 5'-flanking region of the mouse JDP2 gene. Primer extension analysis revealed a new transcription start site (+1). Promoter analysis showed that the region from nt -343 ...
DebRoy Auditi A University of Illinois, United - - 2014
Stromal interacting molecule 1 (STIM1) regulates store-operated Ca2+ entry (SOCE). Here, we show that STIM1 expression in endothelial cells (ECs) is increased during sepsis and thus contributes to hyper-permeability. LPS induced STIM1 mRNA and protein expression in human and mouse lung ECs. The induced STIM1 expression was associated with augmented ...
Boddu Ravindra R Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL, 35294, - - 2014
Autosomal recessive polycystic kidney disease (ARPKD) results from mutations in the human PKHD1 gene. Both this gene, and its mouse ortholog, Pkhd1, are primarily expressed in renal and biliary ductal structures. The mouse protein product, fibrocystin/polyductin complex (FPC), is a 445-kDa protein encoded by a 67-exon transcript that spans >500 kb ...
Grabiner Mark A MA Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA - - 2014
Pseudomonas aeruginosa secrete N-(3-oxododecanoyl)-homoserine lactone (HSL-C12) as a quorum-sensing molecule to regulate bacterial gene expression. Because HSL-C12 is membrane permeant, multiple cell types in P. aeruginosa-infected airways may be exposed to HSL-C12, especially adjacent to biofilms where local (HSL-C12) may be high. Previous reports showed that HSL-C12 causes both pro- ...
Li Yan Y College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, - - 2014
The objective was to investigate the expression of bone morphogenetic protein (BMP) family members in the mouse uterus during the estrous cycle by real-time polymerase chain reaction (PCR) and immunohistochemistry. Uterine samples from Swiss ICR mice were collected and dissected free of surrounding tissue. One uterine horn was snap frozen ...
Soutto Mohammed M Department of Veterans Affairs, Tennessee Valley Healthcare System, Nashville, Tennessee, USA; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee, - - 2014
The expression of TFF1 is frequently down-regulated in human gastric cancer whereas its knockout leads to the development of gastric adenomas and carcinomas in mouse models. The molecular mechanisms underlying the TFF1 tumor suppressor functions remain unclear. In this study, we demonstrate, using colony formation assay and Annexin V staining, ...
Bougault Carole - - 2014
Our objective was to investigate whether a lack of frizzled-related protein B (FrzB), an extracellular antagonist of the Wnt signaling pathways, could enhance cartilage degradation by facilitating the expression, release and activation of matrix metalloproteinases (MMPs) by chondrocytes in response to tissue-damaging stimuli. Cartilage explants from FrzB-/- and wild-type mice ...
Timani Khalid Amine KA From Department of Cell Biology and Immunology, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76107. Electronic address: - - 2014
Tip110 is a nuclear protein and has been shown to function in tumor antigenicity, regulation of gene transcription, pre-mRNA splicing, stem cell proliferation and differentiation, and embryonic development. To characterize the in vivo functions of Tip110, a transgene cassette expressing human Tip110 protein (hTip110) was used to generate hTip110 transgenic ...
Xu Chi C Department of Pharmacology, University of Minnesota Medical School, 6-120 Jackson Hall, 321 Church St. SE, Minneapolis, MN, - - 2014
The mechanism by which addictive drugs such as morphine regulate adult neurogenesis remains elusive. We now demonstrate that morphine can regulate neurogenesis by control of miR-181a and subsequent hippocampal neural progenitor cell (hNPC) lineages. In the presence of morphine, hNPCs preferentially differentiated into astrocytes, an effect blocked by the specific ...
Paatero Ilkka I university of Turku, - - 2014
Conditional knock-out of Hif1a in the mouse mammary gland impairs lobuloalveolar differentiation during lactation. Here, we demonstrate that expression of ErbB4 was reduced in the lobulalveoli of mice with mammary gland-specific deletion of Hif1a. Erbb4 was not, however, a direct target gene for transcriptional regulation by HIF-1α in vitro. HIF-1α ...
Moriya Shunpei S Laboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, - - 2014
Circadian rhythms are related to various psychiatric disorders. Recently, antipsychotics, including quetiapine (QTP), have been accepted as potential therapeutic agents for the treatment of depression, but its mechanism remains poorly understood. In this study, we examined clock gene fluctuation patterns in QTP-treated mice. QTP significantly increased Per2 mRNA at ZT12 ...
Di Emidio Giovanna G Department of Life, Health and Environmental Sciences, University of L'Aquila, Via Vetoio, 67100 L'Aquila, - - 2014
Is SIRT1 involved in the oxidative stress (OS) response in mouse oocytes? SIRT1 plays a pivotal role in the adaptive response of mouse germinal vesicle (GV) oocytes to OS and promotes a signalling cascade leading to up-regulation of the MnSod gene. OS is known to continuously threaten acquisition and maintenance ...
Nagaoka Kentaro K Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Fuchu, - - 2014
In the mouse, L-amino acid oxidase (LAO) produces hydrogen peroxide by utilizing free amino acids and is a proven antibacterial factor in mammary glands. Mastitis, a bacterial infection of the mammary gland, is the most frequent disease in dairy cattle. Here, we investigate whether LAO is expressed in the mammary ...
Forget Antoine A Institut Curie, CNRS UMR 3306, INSERM U1005, Centre Universitaire, Bâtiment 110, 91405 Orsay, - - 2014
Signaling networks controlled by Sonic hedgehog (SHH) and the transcription factor Atoh1 regulate the proliferation and differentiation of cerebellar granule neuron progenitors (GNPs). Deregulations in those developmental processes lead to medulloblastoma formation, the most common malignant brain tumor in childhood. Although the protein Atoh1 is a key factor during both cerebellar ...
Thanasopoulou Angeliki A University Childrens Hospital Basel, - - 2014
The NUP98-NSD1 fusion, product of the t(5;11)(q35;p15.5) chromosomal translocation, is one of the most prevalent genetic alterations in cytogenetically normal paediatric acute myeloid leukaemias and is associated with poor prognosis. Co-existence of a FLT3-ITD activating mutation has been found in more than 70% of NUP98-NSD1-positive patients. To address functional synergism ...
Ye Yaqiong Y College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, - - 2014
MicroRNAs (miRNAs) have been implicated in the process of aging in many model organisms, such as Caenorhabditis elegans, and in many organs, such as the mouse lung and human epididymis. However, the role of miRNAs in the thymus tissues of the aging mouse remains unclear. To address this question, we ...
Zhang Hongxia - - 2014
Perfluorooctanoic acid (PFOA) is a ubiquitous environmental pollutant suspected of being an endocrine disruptor; however, mechanisms of male reproductive disorders induced by PFOA are poorly understood. In this study, male mice were exposed to 0, 0.31, 1.25, 5 and 20 mg PFOA/kg/day by oral gavage for 28 days. PFOA significantly ...
Du Juan J College of Life Sciences, Northwest A&F University, 3 Taicheng Road, Yangling 712100, Shaanxi, - - 2014
SB431542 (SB) is an established small molecular inhibitor that specifically bind to the ATP binding domains of the activin receptor-like kinase receptors, ALK5, ALK4 and ALK7, and thus specifically inhibits Smad2/3 activation and block TGF-β signal transduction. SB maintains the undifferentiated state of mouse embryonic stem cells. However, the way ...
Henderson Colin J CJ Biomedical Research Institute, University of - - 2014
Relatively little progress has been made in determining the in vivo regulation of glutathione S-transferase P (GSTP), particularly the human enzyme hGSTP1, despite being identified as a significant factor in carcinogenesis and development of drug resistance in tumor cell lines. Here we report the characterization of a transgenic reporter mouse ...
Zhang Rui R Department of orthopedics, Wuxi First People's Hospital Affiliated to Nanjing Medical University, Wuxi City, Jiangsu, China, - - 2014
Precartilaginous stem cells (PSCs) are adult stem cells which could initiate chondrocytes and bone growth. In the current study, we purified PSCs from the neonate mice' perichondrial mesenchyme through immunomagnetic beads with the fibroblast growth factor receptor-3 (FGFR-3) antibody. Mouse PSCs were seeded and cultured, and their phenotype was confirmed ...
Chen Ziyan Z State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130, - - 2014
Murine lacrimal, harderian and meibomian glands develop from the prospective conjunctival and eyelid epithelia and produce secretions that lubricate and protect the ocular surface. Sox9 expression localizes to the presumptive conjunctival epithelium as early as E11.5 and is detected in the lacrimal and harderian glands as they form. Conditional deletion ...
Muraoka Naoto N Department of Clinical and Molecular Cardiovascular Research, Keio University School of Medicine, Shinjuku-ku Tokyo, Japan Department of Cardiology, Keio University School of Medicine, Shinjuku-ku Tokyo, - - 2014
Fibroblasts can be directly reprogrammed into cardiomyocyte-like cells (iCMs) by overexpression of cardiac transcription factors or microRNAs. However, induction of functional cardiomyocytes is inefficient, and molecular mechanisms of direct reprogramming remain undefined. Here, we demonstrate that addition of miR-133a (miR-133) to Gata4, Mef2c, and Tbx5 (GMT) or GMT plus Mesp1 ...
Wan Xiaoling X Institute of Biology and Medical Sciences, Soochow University, 199 Renai Road, Nanjing, Jiangsu, 215123, - - 2014
Trithorax group (TrxG) proteins play critical roles in transcriptional activation by promoting methylation of histone H3 Lysine 4 (H3K4), but the precise functions of the individual TrxG members during embryonic differentiation are not fully understood. Here we show that Mll2, a TrxG member, is required for proliferation but is dispensable ...
Wu Yanru Y Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei, 430079, People's Republic of - - 2014
Induced pluripotent stem cells (iPSCs) have great potential in bone tissue engineering to repair large bone defects. Before their clinical application, investigations are needed to discover the genes and osteoconductive scaffolds that influence their differentiation toward an osteogenic lineage. Alox5 plays controversial and complex roles in the regulation of bone ...
Jin Li-Yan LY Department of Infectious Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, P.R. - - 2014
The aim of the present study was to explore the protective effect of small interfering RNA (siRNA) against nuclear factor κB (NF-κB) p65 on sepsis-induced acute lung injury (ALI) in mice. In total, 70 male Kunming mice were randomly divided into a healthy control group, a sepsis group, a specific interfering ...
Guerrero Lucia L Departamento de Bioquímica, Facultad de Medicina, Universidad Autónoma de Madrid, Instituto de Investigaciones Biomédicas Alberto Sols, C.S.I.C., Madrid, - - 2014
Fast and slow TnI are co-expressed in E11.5 embryos, and fast TnI is present from the very beginning of myogenesis. A novel green fluorescent protein (GFP) reporter mouse lines (FastTnI/GFP lines) that carry the primary and secondary enhancer elements of the mouse fast troponin I (fast TnI), in which reporter ...
Xie Liwei L Department of Molecular & Integrative - - 2014
Caveolae are specialized microdomains on membranes that are critical for signal transduction, cholesterol transport and endocytosis. Caveolin-1 (CAV1) is a multi-functional protein and a major component of caveolae. Cav1 is directly activated by hypoxia-inducible factor (HIF). HIFs are heterodimers of an oxygen sensitive α-subunit, HIF1α or HIF2α, and a constitutively ...
Panda Amaresh C AC Laboratory of - - 2014
The mammalian RNA-binding protein AUF1 (AU-binding factor 1, also known as heterogeneous nuclear ribonucleoprotein D, hnRNP D) binds to numerous mRNAs and influences their post-transcriptional fate. Given that many AUF1 target mRNAs encode muscle-specific factors, we investigated the function of AUF1 in skeletal muscle differentiation. In mouse C2C12 myocytes, where ...
Dahlhoff Maik M Gene Center, LMU Munich, Munich, - - 2014
The epidermal growth factor receptor (EGFR) system is a key regulator of epithelial development and homeostasis. Its functions in the sebaceous gland (SG), however, remain poorly characterized. Here, using a transgenic mouse line with tissue-specific and inducible expression of the EGFR ligand epigen, we show that increased activation of the ...
Wang Yun Y Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, - - 2014
Abrus mollis is a widely used traditional Chinese medicine for treating acute and chronic hepatitis, steatosis, and fibrosis. It was found that the total flavonoid C-glycosides from Abrus mollis extract (AME) showed potent antioxidant, anti-inflammatory, and hepatoprotective activities. To further investigate the hepatoprotective effect of AME and its possible mechanisms, ...
Yoon Hyunho H R&D Center, Cellbiotech Co. Ltd., Gimpo, - - 2014
[This corrects the article on p. 27 in vol. 30, PMID: 24795796.].
Padmakumar V C - - 2014
Chloride Intracellular Channel 4 (CLIC4) is one of seven members in the closely related CLIC protein family. CLIC4 is involved in multiple cellular processes including apoptosis, cellular differentiation, inflammation and endothelial tubulogenesis. Despite over a decade of research, no comprehensive in situ expression analysis of CLIC4 in a living organism ...
Wang Liya L Henan Eye Institute & Henan Eye Hospital, Zhengzhou, Henan, 450003, - - 2014
Purpose. Retinopathy of prematurity (ROP) is directly associated with abnormal expression of retinal vascular endothelial growth factor (VEGF) in premature neonates. This study was to investigate whether the systemic administration of retinoic acid (RA) regulates retinal VEGF expression, and prevents retinal neovascularization and retinopathy in the oxygen-induced retinopathy (OIR) mouse ...
Wang Tiancheng T Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, People's Republic of - - 2014
Mastitis is an inflammatory disease caused by microbial infection. Staphylococcus aureus is one of the primary bacteria responsible for mastitis. Stevioside is isolated from Stevia rebaudiana and is known to have therapeutic functions. This study was designed to evaluate the effects of stevioside in a mouse model of S. aureus-induced ...
Yin Xinguang X Department of Gastroenterology, The First Affiliated Hospital of Jiaxing College, 1882 Central-South Road, Jiaxing, 314001, Zhejiang Province, People's Republic of China, - - 2014
The development of liver fibrosis is the fundamental stage toward a number of mortal complications of liver diseases, including cirrhosis and hepatocellular carcinoma. Canonical Wnt pathway is crucial in diverse biological processes and mediates the progression and regression of liver fibrosis. As a potent Wnt pathway agonist, the role of ...
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