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Nader Marina M Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Campus Universitário-Trindade, Florianópolis, Santa Catarina, 88040-970, - - 2014
This study was conducted to explore the anti-inflammatory effect of Jungia sellowii (Asteraceae) using a murine model of pleurisy induced by carrageenan (Cg). This plant is used in southern Brazil to treat inflammatory diseases. J. sellowii leaves were extracted with ethanol/water to obtain the crude extract (CE), which was fractionated ...
Layat Elodie E UMR 7622, Sorbonne Universités, UPMC Univ Paris 06, 75005, Paris, France; UMR 7622, CNRS, 75005, Paris, - - 2014
Seed dormancy, which blocks germination in apparently favourable conditions, is a key regulatory control point of plant population establishment. As germination requires de novo translation, its regulation by dormancy is likely to be related to the association of individual transcripts to polysomes. Here, the polysome-associated mRNAs, that is, the translatome, ...
Ruas Margarida M Department of Pharmacology, Oxford University, UK margarida.ruas@pharm.ox.ac.uk - - 2014
Organelle ion homeostasis within the endo-lysosomal system is critical for physiological functions. TPCs (Two-Pore Channels) are cation channels that reside in endo-lysosomal organelles and over-expression results in endo-lysosomal trafficking defects. However, the impact of lack of TPC expression on endo-lysosomal trafficking is unknown. Here, we characterize Tpcn1 expression in two ...
Lee Sueun S College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, Republic of - - 2014
Nestin is a protein of embryonic intermediate filaments expressed by multipotent neural stem cells. In the present study, the nestin expression pattern in the mouse hippocampus 1, 2, 3, 4, and 8 days after treatment with trimethyltin (TMT) was examined to explore the possible role played by nestin in chemically ...
Elraghy Omaima O Biological Sciences, Clemson University, 132 Long Hall, Clemson, SC, 29634, - - 2014
The Cyp2d subfamily is the second most abun-dant subfamily of hepatic drug-metabolizing CYPs. In mice, there are nine Cyp2d members that are believed to have redundant catalytic activity. We are testing and optimizing the ability of one short interfering RNA (siRNA) construct to knockdown the expression of multiple mouse Cyp2ds ...
Zhang Wei W College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of - - 2014
Macrophage migration inhibitory factor (MIF) functions as a pleiotropic protein, participating in a vast array of cellular and biological processes. Abnormal expression of MIF has been implicated in many neurological diseases, including Parkinson's disease, epilepsy, Alzheimer's Disease, stroke, and neuropathic pain. However, the expression patterns of mif transcript and MIF ...
Xu Xu X Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY, - - 2014
Fat-specific protein 27 (Fsp27) is a lipid droplet-associated protein that promotes lipid droplet (LD) growth and triglyceride (TG) storage in white adipocytes. Fsp27 is also highly expressed in the steatotic liver and contributes to TG accumulation. In this study, we discovered that the liver produces Fsp27β, an alternative Fsp27 isoform, ...
Jeong Ji-Hye JH Department of Biomedical Science, College of Medicine CHA University, Seoul 135-081, Korea, Republic of - - 2014
Aimp1 is known as a multifunctional cytokine in various cellular events. Recent study showed Aimp1 is localized in glandular epithelial, endothelial, and stromal cells in functionalis and basalis layers of the endometrium. However, the regulatory mechanism of Aimp1 in the uterus remains unknown. In the present study, we found that ...
Moriyama Ryutaro R Laboratory of Molecular Neurobiology, Department of Life Science, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka, 577-8502, Japan, - - 2014
G-protein-coupled receptor 120 (GPR120) has been known to be a receptor of long-chain fatty acids. Here, we investigated GPR120 expression in the mouse pituitary gland via real-time PCR, in situ hybridization, and immunohistochemistry. GPR120 mRNA was abundantly expressed in the pituitary gland of ad-lib fed animals. In situ hybridization and ...
Weng Shunyan S Shanghai Key Laboratory of Veterinary Biotechnology; College of Agriculture and Biology; Shanghai Jiao Tong University; Shanghai, P.R. - - 2014
Secreted phosphoprotein 1 (SPP1) is a multifunctional protein expressed by cells from a large variety of tissues. It is involved in many physiological and pathological processes, including bone metabolism, inflammation progress, tumor metastasis, injury repair, hyperoxia-induced injury, and so on. Native SPP1 from multiple species have been isolated from the ...
Wu Alex Man Lai AM The Hospital for Sick - - 2014
The multidrug resistance efflux transporter ATP-binding cassette subfamily G member 2 (ABCG2) is not only overexpressed in certain drug resistant cancers but is also highly expressed in the mammary gland during lactation, carrying xenobiotics and nutrients into milk. We sought to investigate the molecular mechanisms involved in the upregulation of ...
Marotta Pina P IRGS, Biogem, Via Camporeale, Ariano Irpino, 83031, Avellino, - - 2014
The transcription factor Pax8 is already known to be essential at very early stages of mouse thyroid gland development, before the onset of thyroid hormone production. In this paper we show, using a conditional inactivation strategy, that the removal of the Pax8 protein late in gland development results in severe ...
Kim Jinwook J College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, South - - 2014
Brain-derived neurotrophic factor (BDNF) is regarded as an important factor for neurogenesis, synaptic plasticity, and neuronal network organization in brain circuits. However, little is known about the regulation of BDNF transcript variants in the hippocampus during postnatal development and following chemically induced neurotoxicity. In the present study, we examined the ...
Nagalingam Raghu S RS University of Illinois, Chicago, United - - 2014
Understanding the regulation of cardiac fibrosis is critical for controlling adverse cardiac remodeling during heart failure. Previously we identified miR-378 as a cardiomyocytes-abundant miRNA down-regulated in several experimental models of cardiac hypertrophy and in patients with heart failure. To understand the consequence of miR-378 down-regulation during cardiac remodeling, our current ...
Ihara Kan-Ichiro KI Laboratory of Molecular Biology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, - - 2014
Membrane-associated guanylate kinase inverted 2 (MAGI-2) is a tight junction protein in epithelial tissues. We previously reported the detailed expression patterns of MAGI-2 in mouse tissues, including kidney podocytes, based on results obtained from Venus knock-in mice for Magi2 locus. In the present study, homozygous deletion of the Magi2 gene ...
Bardot B B 1] Institut Curie, Centre de recherche, Genetics of Tumor Suppression (Equipe Labellisée Ligue 2014), Paris, France [2] UPMC Univ Paris 06, Paris, France [3] CNRS UMR 3244, Paris, - - 2014
Mdm4, a protein related to the ubiquitin-ligase Mdm2, is an essential inhibitor of tumor suppressor protein p53. In both human and mouse cells, the Mdm4 gene encodes two major transcripts: one encodes the full-length oncoprotein (designated below as Mdm4-FL), whereas the other, resulting from a variant splicing that skips exon ...
Roque-Ramírez Bladimir B División de Investigación Biomédica, Subdirección de Enseñanza e Investigación, Centro Médico Nacional 20 de Noviembre, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., - - 2014
Background: Type 2D limb-girdle muscular dystrophy (LGM2D) is a progressive disorder caused by mutations in the alpha sarcoglycan (α-SG) gene. In mice, the α-SG gene contains two promoters that regulate the expression of two different mRNAs (A and B). However, their gene expression pattern during embryonic development has not been ...
Roy-Chaudhuri Biswajoy B 1] Department of Pediatrics, Stanford University, Stanford, California, USA. [2] Department of Genetics, Stanford University, Stanford, California, - - 2014
Processing of microRNAs (miRNAs) from their precursors to their biologically active mature forms is regulated during development and cancer. We show that mouse pri- or pre-miR-151 can bind to and compete with mature miR-151-5p and miR-151-3p for binding sites contained within the complementary regions of the E2f6 mRNA 3' untranslated ...
Panigrahy Dipak D 1Vascular Biology Program, Boston Children's Hospital 2Department of Surgery, Harvard Medical School 3Center for Vascular Biology Research, Beth Israel Deaconess Medical Center, Boston, MA, United States 4Department of Surgery, Klinikum Grosshadern, LMU Munich, Munich, Germany 5Department of Ophthalmology, Harvard Medical School 6Department of Pathology, Harvard Medical School 7Schepens Eye Research Institute, Massachusetts Eye & Ear, Boston, MA, United - - 2014
Neuropilin-1 (NRP1) is a receptor for vascular endothelial growth factor (VEGF). A soluble isoform of Nrp1 (sNrp1) has not been described in the mouse. Our goal was to examine the expression of mouse sNrp1 during liver development and regeneration.sNrp1 was cloned from mouse liver. The expression of sNrp1 and VEGF ...
Reeh Kaitlin A G KA Department of Molecular Carcinogenesis, University of Texas, M.D. Anderson Cancer Center, Science Park Research Division, Smithville, TX 78957, - - 2014
The thymus and parathyroid glands arise from a shared endodermal primordium in the third pharyngeal pouch (3rd pp). Thymus fate is specified in the ventral 3rd pp between E9.5 and E11, whereas parathyroid fate is specified in the dorsal domain. The molecular mechanisms that specify fate and regulate thymus and ...
Tanaka Yuka Y Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan Center for Clinical and Translational Research, Kyushu University Hospital, Fukuoka, - - 2014
Docking protein 2 (Dok2) is an adapter protein which is involved in hematopoiesis. However, it still remains unclear how Dok2 functions in regulation of transcription of hematopoietic genes. To address this issue, we knocked-down Dok2 mRNA in mouse erythroleukemia cells which highly express Dok2 intrinsically. Mouse erythroleukemia cells were transfected ...
Wang David H - - 2014
Metaplasia can result when injury reactivates latent developmental signaling pathways that determine cell phenotype. Barrett's esophagus is a squamous-to-columnar epithelial metaplasia caused by reflux esophagitis. Hedgehog (Hh) signaling is active in columnar-lined, embryonic esophagus and inactive in squamous-lined, adult esophagus. We showed previously that Hh signaling is reactivated in Barrett's ...
Maurer Kate A KA Division of Developmental Biology, Cincinnati Children's Hospital Research Foundation, Cincinnati, OH 45229, - - 2014
Notch signaling regulates basic helix-loop-helix (bHLH) factors as an evolutionarily conserved module, but the tissue-specific mechanisms are incompletely elucidated. In the mouse retina, bHLH genes Atoh7 and Neurog2 have distinct functions, with Atoh7 regulating retinal competence and Neurog2 required for progression of neurogenesis. These transcription factors are extensively co-expressed, suggesting ...
Jin Chengri C Department of Orthodontics, Yonsei University College of Dentistry, 50 Yonsei-Ro,, Seodaemun-Gu,, Seoul,, 120-752, - - 2014
Mammalian palate development is regulated by complex processes. Many cellular and molecular events, such as cell proliferation, apoptosis, cell migration and the epithelial mesenchymal transition, regulate proper palate development and some abnormalities in palate development lead to cleft palate. Various developmental disorders, such as cleft palate and disorders of the ...
Polito Vinicia A VA Huffington Center on Aging, Baylor College of Medicine, Houston, TX, - - 2014
Accumulating evidence implicates impairment of the autophagy-lysosome pathway in Alzheimer's disease (AD). Recently discovered, transcription factor EB (TFEB) is a molecule shown to play central roles in cellular degradative processes. Here we investigate the role of TFEB in AD mouse models. In this study, we demonstrate that TFEB effectively reduces ...
Zhao Xiaoyun X Clinical Laboratory, Hebei General Hospital, Shijiazhuang, Hebei 050057, P.R. - - 2014
MicroRNAs (miRNAs) are a class of non‑coding 18‑25-nucleotide endogenous RNA molecules. miRNAs act as specific gene silencers to regulate target gene expression at the posttranscriptional level, by base pairing to the 3'‑untranslated region of the target mRNA. miR‑152 is an miRNA that was originally identified in cancer cells, and was ...
Zhou Yan Y Department of Neurobiology, Mount Sinai School of Medicine, New York, NY 10029. Electronic address: - - 2014
In humans, alcoholism is associated with a decrease in basal ACTH and cortisol levels, and blunted pituitary ACTH responses to administered corticotropin-releasing hormone (CRH) during active drinking and after long-term abstinence. Preclinical studies indicate that a persistent decrease in pituitary activation after chronic exposure to ethanol is due to a ...
Rentsendorj Otgonchimeg O Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins Medical Institutions, Baltimore, Maryland, - - 2014
PDE2A is a dual-function PDE that is stimulated by cGMP to hydrolyze cAMP preferentially. In a two-hit model of ALI, we found previously that PDE2A decreased lung cAMP, up-regulated lung iNOS, and exacerbated ALI. Recent data suggest that macrophage iNOS expression contributes to ALI but later, promotes lung-injury resolution. However, ...
Zhang Deqiang D University of Michigan Medical School, United - - 2014
The clock protein BMAL1 participates in circadian regulation of lipid metabolism, but its contribution to insulin-AKT-regulated hepatic lipid synthesis is unclear. Here we used both Bmal1-/- and acute liver-specific Bmal1-depleted mice to study the role of BMAL1 in refeeding-induced de novo lipogenesis in the liver. Both global deficiency and acute ...
Shiizaki Kazuhiro K National Cancer Center Research - - 2014
Omeprazole (OME), a proton pump inhibitor used to treat gastritis, is also an aryl hydrocarbon receptor (AhR) activator. OME activates AhR in human hepatocytes and hepatoma cells but not in mouse in vivo or in vitro. We recently discovered that this species-specific difference results from a difference in a few ...
Zhu Hong-Y HY *Singapore Eye Research Institute, Singapore, Singapore; and †SRP Neuroscience and Behavioral Disorders, Duke-NUS, Singapore, - - 2014
The aim of this study was to compare the expression patterns of 3 important biochemical characteristics of fibrosis-moesin, transforming growth factor (TGF)-β1, and α-smooth muscle actin (SMA) in the mouse cornea with fibrosis induced by common etiologies-sterile mechanical injury and infection. Corneas of 8-week-old C57BL6 mice were either wounded after ...
Makkar A A Magee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA, USA; Department of Pediatrics, University of Pittsburgh, - - 2014
Fatty Acid Binding Protein-4 (FABP4) is a member of a family of FABP proteins that regulate intracellular lipid trafficking in diverse tissues. We recently showed that FABP4 regulates triglyceride accumulation in primary human trophoblasts. To assess the function of placental FABP4 in vivo, we tested the hypothesis that FABP4 is expressed ...
Nakajima Akira A Department of Orthodontics, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo 1018310, Japan; Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo 1018310, Japan. Electronic address: - - 2014
Reported expression patterns for TGF-β receptors (TβR-I, -II, and -III) during palatogenesis suggest that they play essential roles in the mechanisms leading to palatal fusion. The purpose of this study was to compare the functions of the three TβRs during palatal fusion. Using organ culture of mouse palatal shelves, expression ...
Best Sarah A SA ACRF Stem Cells and Cancer Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia Department of Medical Biology, The University of Melbourne, Parkville, Victoria 3010, - - 2014
The HLH transcriptional regulator Id4 exerts important roles in different organs, including the neural compartment, where Id4 loss usually results in early lethality. To explore the role of this basally restricted transcription factor in the mammary gland, we generated a cre-inducible mouse model. MMTV- or K14-cre-mediated deletion of Id4 led ...
Mao Hui Z HZ Cedars-Sinai Medical Center, United - - 2014
Lipase maturation factor 1 (Lmf1) is a critical determinant of plasma lipid metabolism, as demonstrated by severe hypertriglyceridemia associated with its mutations in mice and human subjects. Lmf1 is a chaperone localized to the ER and required for the post-translational maturation and activation of several vascular lipases. Despite its importance ...
Garcia Virginia B VB Department of Biological Sciences, University of Missouri, Columbia, MO USA - - 2014
Neural networks ultimately arrive at functional output via interaction of the excitability of individual neurons and their synaptic interactions. We investigated the relationships between voltage-gated ion channel and neurotransmitter receptor mRNA levels in mouse spinal cord at four different postnatal time points (P5, P11, P17, and adult) and three different ...
Szafranski Karol K Fritz Lipmann Institute-Institute for Age Research, Beutenbergstr. 11, 07745 Jena, Germany - - 2014
Thousands of tandem alternative splice sites (TASS) give rise to mRNA insertion/deletion variants with small size differences. Recent work has concentrated on the question of biological relevance in general, and the physiological regulation of TASS in particular. We have quantitatively studied 11 representative TASS cases in comparison to one mutually ...
Xu Yuanhong Y Department of Pancreatic Gastroenterologic Surgery, First Hospital of China Medical University, No. 92, Nanjing Rd, Shenyang, 110001, China. Electronic address: - - 2014
Jun dimerization protein 2 (JDP2) is a repressor of transcription factor AP-1. To investigate the transcriptional regulation of the JDP2 gene, we cloned the 5'-flanking region of the mouse JDP2 gene. Primer extension analysis revealed a new transcription start site (+1). Promoter analysis showed that the region from nt -343 ...
DebRoy Auditi A University of Illinois, United - - 2014
Stromal interacting molecule 1 (STIM1) regulates store-operated Ca2+ entry (SOCE). Here, we show that STIM1 expression in endothelial cells (ECs) is increased during sepsis and thus contributes to hyper-permeability. LPS induced STIM1 mRNA and protein expression in human and mouse lung ECs. The induced STIM1 expression was associated with augmented ...
Boddu Ravindra R Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL, 35294, - - 2014
Autosomal recessive polycystic kidney disease (ARPKD) results from mutations in the human PKHD1 gene. Both this gene, and its mouse ortholog, Pkhd1, are primarily expressed in renal and biliary ductal structures. The mouse protein product, fibrocystin/polyductin complex (FPC), is a 445-kDa protein encoded by a 67-exon transcript that spans >500 kb ...
Grabiner Mark A MA Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA - - 2014
Pseudomonas aeruginosa secrete N-(3-oxododecanoyl)-homoserine lactone (HSL-C12) as a quorum-sensing molecule to regulate bacterial gene expression. Because HSL-C12 is membrane permeant, multiple cell types in P. aeruginosa-infected airways may be exposed to HSL-C12, especially adjacent to biofilms where local (HSL-C12) may be high. Previous reports showed that HSL-C12 causes both pro- ...
Li Yan Y College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, - - 2014
The objective was to investigate the expression of bone morphogenetic protein (BMP) family members in the mouse uterus during the estrous cycle by real-time polymerase chain reaction (PCR) and immunohistochemistry. Uterine samples from Swiss ICR mice were collected and dissected free of surrounding tissue. One uterine horn was snap frozen ...
Soutto Mohammed M Department of Veterans Affairs, Tennessee Valley Healthcare System, Nashville, Tennessee, USA; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee, - - 2014
The expression of TFF1 is frequently down-regulated in human gastric cancer whereas its knockout leads to the development of gastric adenomas and carcinomas in mouse models. The molecular mechanisms underlying the TFF1 tumor suppressor functions remain unclear. In this study, we demonstrate, using colony formation assay and Annexin V staining, ...
Bougault Carole - - 2014
Our objective was to investigate whether a lack of frizzled-related protein B (FrzB), an extracellular antagonist of the Wnt signaling pathways, could enhance cartilage degradation by facilitating the expression, release and activation of matrix metalloproteinases (MMPs) by chondrocytes in response to tissue-damaging stimuli. Cartilage explants from FrzB-/- and wild-type mice ...
Timani Khalid Amine KA From Department of Cell Biology and Immunology, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76107. Electronic address: - - 2014
Tip110 is a nuclear protein and has been shown to function in tumor antigenicity, regulation of gene transcription, pre-mRNA splicing, stem cell proliferation and differentiation, and embryonic development. To characterize the in vivo functions of Tip110, a transgene cassette expressing human Tip110 protein (hTip110) was used to generate hTip110 transgenic ...
Xu Chi C Department of Pharmacology, University of Minnesota Medical School, 6-120 Jackson Hall, 321 Church St. SE, Minneapolis, MN, - - 2014
The mechanism by which addictive drugs such as morphine regulate adult neurogenesis remains elusive. We now demonstrate that morphine can regulate neurogenesis by control of miR-181a and subsequent hippocampal neural progenitor cell (hNPC) lineages. In the presence of morphine, hNPCs preferentially differentiated into astrocytes, an effect blocked by the specific ...
Paatero Ilkka I university of Turku, - - 2014
Conditional knock-out of Hif1a in the mouse mammary gland impairs lobuloalveolar differentiation during lactation. Here, we demonstrate that expression of ErbB4 was reduced in the lobulalveoli of mice with mammary gland-specific deletion of Hif1a. Erbb4 was not, however, a direct target gene for transcriptional regulation by HIF-1α in vitro. HIF-1α ...
Moriya Shunpei S Laboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, - - 2014
Circadian rhythms are related to various psychiatric disorders. Recently, antipsychotics, including quetiapine (QTP), have been accepted as potential therapeutic agents for the treatment of depression, but its mechanism remains poorly understood. In this study, we examined clock gene fluctuation patterns in QTP-treated mice. QTP significantly increased Per2 mRNA at ZT12 ...
Di Emidio Giovanna G Department of Life, Health and Environmental Sciences, University of L'Aquila, Via Vetoio, 67100 L'Aquila, - - 2014
Is SIRT1 involved in the oxidative stress (OS) response in mouse oocytes? SIRT1 plays a pivotal role in the adaptive response of mouse germinal vesicle (GV) oocytes to OS and promotes a signalling cascade leading to up-regulation of the MnSod gene. OS is known to continuously threaten acquisition and maintenance ...
Nagaoka Kentaro K Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Fuchu, - - 2014
In the mouse, L-amino acid oxidase (LAO) produces hydrogen peroxide by utilizing free amino acids and is a proven antibacterial factor in mammary glands. Mastitis, a bacterial infection of the mammary gland, is the most frequent disease in dairy cattle. Here, we investigate whether LAO is expressed in the mammary ...
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