Search Results
Results 1 - 50 of 2038
1 2 3 4 5 6 7 8 9 10 >
Mohapatra S K SK 1Animal Biotechnology Centre,National Dairy Research - - 2014
Insulin-like growth factor binding protein-5 (IGFBP-5) is a key molecule in mammary gland development, which facilitates the removal of mammary epithelial cells (MECs) by apoptosis that takes place during remodeling of the mammary gland during involution. IGFBP-5 binds with IGFs for their bioavailability. IGFBP-5 has been reported to perform pleiotropic ...
Blais M M Dairy and Swine R & D - - 2014
Pathogenic invasion by Escherichia coli and Salmonellae remains a constant threat to the integrity of the intestinal epithelium and can rapidly induce inflammatory responses. At birth, colostrum consumption exerts numerous beneficial effects on the properties of intestinal epithelial cells and protects the gastrointestinal tract of newborns from pathogenic invasion. The ...
Tsiplakou Eleni E Department of Nutritional Physiology and Feeding,Agricultural University of Athens,Iera Odos 75, - - 2014
Milk fatty acid (FA) synthesis by the mammary gland involves expression of a large number of genes whose nutritional regulation remains poorly defined. In this study, we examined the effect of long-term under- and over-feeding on the expression of genes (acetyl Co A carboxylase, ACC; fatty acid synthetase, FAS; lipoprotein ...
Cho H H College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, South - - 2014
Transmembranous calcium (Ca(2+)) channels such as Trpv5/6,Ncx1,Pmca1b are known to play an important role in maintaining homeostasis and metabolizing Ca(2+) ions. Transient receptor potential cation channel subfamily V members 5 and 6 (Trpv5/6) play an important role in Ca(2+) absorption. Na(+)/Ca(2+) exchanger 1 (Ncx1) and plasma membrane Ca(2+)-transporting ATPase1 (Pmca1b) ...
Nahar Asrafun - - 2014
Oviducts synthesise macrophage migration inhibitory factor (MIF) to promote sperm capacitation and embryogenesis. This study aimed to test a hypothesis that the oviducts of obese cows may express MIF at a lower level than those of normal and lean cows. Ampullar and isthmic oviduct sections were collected from lean (n=5; ...
Lee Mihye M Center for RNA Research, Institute for Basic Science, Seoul 151-742, Korea; School of Biological Sciences, Seoul National University, Seoul 151-742, - - 2014
Early development depends heavily on accurate control of maternally inherited mRNAs, and yet it remains unknown how maternal microRNAs are regulated during maternal-to-zygotic transition (MZT). We here find that maternal microRNAs are highly adenylated at their 3' ends in mature oocytes and early embryos. Maternal microRNA adenylation is widely conserved ...
De Luca A A Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria, Naples, 80137, - - 2014
Aquaporin-1 (AQP1), a six-transmembrane domain protein, belongs to a highly conserved group of proteins called aquaporins known to regulate permeability across cell membranes. Although the role of AQP1 has been extensively studied, its specific activity along the gastrointestinal tract in animals during early postnatal development is poorly known. This study ...
Fontes P K PK Department of Pharmacology, Institute of Bioscience, University of São Paulo State, Botucatu, São Paulo, - - 2014
Many peptides are responsible for the coordination of muscle contraction, secretion and ciliary beating of the oviduct epithelium to allow the transport of gametes and embryos, including vascular endothelial growth factors (VEGF), prostaglandins (PGs), endotelin-1 (ET-1) and angiotensin II (Ang II). The effect of reproductive biotechnologies used to improve embryo ...
Thompson I M T IM Department of Animal Sciences, University of Florida, Gainesville - - 2014
Heat stress in the dry period affects the immune status of dairy cows in the subsequent lactation. We hypothesized that cooling during the dry period improves immune response to postpartum intramammary infection (IMI) by environmental pathogens such as Streptococcus uberis. Cows were dried off 46 d before expected calving and ...
Piamya P P 1Department of Animal Science,National Chung Hsing University,Taichung 402,Taiwan,Republic of - - 2014
The contribution of matrix metalloproteinases (MMP) to timely discharge of the placenta from bovine uterus at parturition is yet inconclusive, partly because of the presence of multiple MMP forms in situ. In the current study, the expression of different gelatinase subtypes on non-retaining placentas of Holstein cows was fingerprinted by ...
Zhao Feng F Key laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin, 150030, Heilongjiang, - - 2014
In dairy cows, the extracellular microenvironment varies significantly from the virgin state to lactation. The function of integrin α6β4 is dependent on cell type and extracellular microenvironment, and the precise expression profile of α6β4 and its effects on mammary development remain to be determined. In the present study, real-time PCR ...
Uejyo Takaaki T Laboratory of Dairy Food Science, Research Faculty of Agriculture, Hokkaido University, North 9, West 9, Sapporo, 060-8589, - - 2014
The mammary gland is a highly specialized organ that is able to repeat development and regression (involution) of alveolar structures for milk production. Mammary involution consists in two phases. The first phase is reversible and lasts until approximately 48 h after weaning in mice. Interestingly, an extended milking interval can change ...
Langley Alexander R AR MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, - - 2014
The initial phases of embryonic development occur in the absence of de novo transcription and are instead controlled by maternally inherited mRNAs and proteins. During this initial period, cell cycles are synchronous and lack gap phases. Following this period of transcriptional silence, zygotic transcription begins, the maternal influence on development ...
de Moraes Carolina N CN Department of Animal Reproduction and Radiology, São Paulo State University-UNESP, Botucatu, São Paulo, Brazil. Electronic address: - - 2014
The use of cloprostenol sodium in puerperium is questionable, as both favorable and unfavorable responses during the uterine involution process have been reported in the literature. This study is based on the hypothesis that cloprostenol sodium promotes modifications in the prostaglandin F2α receptor (FP), caspase 3 (CASP-3), and cyclooxygenase 2 ...
Dai L L Laboratory Animal Center, College of Animal Sciences, Jilin University, Changchun, - - 2014
Although clear advances have been made in understanding of gene transcriptional regulation in the corpous luteum (CL) during the oestrous cycle, little is known about the involvement of microRNAs (miRNAs) in this physiological process. In the present study, expression of miR-126-3p was preliminarily detected in bovine CL throughout the oestrous ...
Goissis Marcelo D MD Department of Animal Science, Michigan State University, East Lansing, Michigan; Capes Foundation, Ministry of Education, Brasília, - - 2014
Placental defects are common in bovine embryos produced using assisted reproductive techniques. A proper understanding of the events leading to inner cell mass (ICM) and trophectoderm (TE) specification could help identify the origins of such developmental failures. We focused on caudal-type homeobox transcription factor 2 (CDX2) since it has a ...
Nazemi Sasan S Department of Veterinary Clinical and Animal Science (IKVH), Faculty of Health and Medical Sciences,University of - - 2014
We used a mouse model of pathogenic (Staphylococcus aureus) and non-pathogenic (teat sealing) mammary inflammation to investigate mRNA expression of several inflammatory cytokines and acute phase proteins (APP) in mammary tissue and liver, and the appearance of some of these factors in plasma and milk. The expression levels of IL1β ...
Palomares Roberto A RA Department of Population Health, College of Veterinary Medicine, University of Georgia, 425 River Rd, Athens, GA, 30602, USA, - - 2014
The objective of this study was to compare the mRNA expression of Toll-like receptors (TLR3 and TLR7), and costimulatory molecules involved in activation of lymphocytes and antigen presenting cells (CD80, CD86, CD28, and CD40L) after experimental infection of beef calves with low or high virulence noncytopathic (ncp) bovine viral diarrhea ...
Sun Xiaoyong X Shandong Agricultural University; - - 2014
Alternative splicing is an essential biological process to generate proteome diversity and phenotypic complexity. Recent improvements in RNA sequencing (RNA-seq) accuracy and computational algorithms have provided unprecedented opportunities to examine the expression level of Arabidopsis transcripts. In this paper, we analyzed 61 RNA-seq samples from 10 totally independent studies of ...
Bakhtari Azizollah A Department of Animal Science; University of California; Davis, CA USA; Department of Animal Science; Isfahan University of Technology; Isfahan, - - 2014
Dppa3 has been described in mice as an important maternal factor contributed by the oocyte that participates in protecting the maternal genome from oxidation of methylated cytosines (5mC) to hydroxymethylated cytosines (5hmC). Dppa3 is also required for normal mouse preimplantation development. This gene is poorly conserved across mammalian species, with ...
Afonina Zhanna A ZA Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, - - 2014
The polyribosomes newly formed on recombinant GFP-encoding mRNAs in a wheat germ cell-free translation system were analyzed using cryo-electron tomography, with sub-tomogram averaging of polysomal ribosomes and reconstruction of 3D structures of individual polyribosomes. The achieved level of resolution in the reconstructed polyribosomes allowed deducing the mRNA path by connecting ...
Bai Na N From the *State Key Laboratory of Oral Disease, Sichuan University, Chengdu, Sichuan; †Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan; ‡Department of Oral and Maxillofacial & Head and Neck Surgery, Medical College and Affiliated Hospital, Qingdao University, Qingdao; and §Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Tianjin Medical University, Tianjin, People's Republic of - - 2014
Because cleft palate (CP) is one of the most common congenital deformities, surgeons have tried for longtime to achieve an ideal reconstruction of the palatal bone defects and restoration of muscle attachments. In this study, a new CP model on rhesus was established and corrected by an approach of distraction ...
Castilho A C S AC Department of Pharmacology, Institute of Bioscience, University of São Paulo State, Botucatu, São Paulo, - - 2014
The LH plays a key role in controlling physiological processes in the ovary acting via LH receptor (LHR). In general, the effects of LHR on the regulation of granulosa cell differentiation are mediated mainly via the Gs-protein/adenylyl cyclase/cAMP system; however, the LHR activation could also induce phospholipase C (PLC)/inositol trisphosphate ...
Gallego Romero Irene - - 2014
The use of low quality RNA samples in whole-genome gene expression profiling remains controversial. It is unclear if transcript degradation in low quality RNA samples occurs uniformly, in which case the effects of degradation can be corrected via data normalization, or whether different transcripts are degraded at different rates, potentially ...
Kaplan David D Pathfinder Biotech, Cleveland, OH; Department of Pathology, University Hospitals Case Medical Center and Case Western Reserve University, Cleveland, - - 2014
The E2997 Phase III trial included preservation of valuable chronic lymphocytic leukemia (CLL) patient specimens and relevant clinical outcome data. Using a novel high-resolution technology on a flow cytometry platform, we assessed 79 E2997 samples for the expression of 27 analytes that reflected the activity of signaling pathways and apoptosis. ...
Buckley Martin S MS Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA. Electronic address: - - 2014
Over the past twenty years, exciting developments in optical and molecular imaging approaches have allowed researchers to examine with unprecedented resolution the spatial organization of transcription sites in the nucleus. An attractive model that has developed from these studies is that active genes cluster to preformed transcription factories that contain ...
Müller Adrienne A Center for Radiopharmaceutical Sciences, ETH Zurich, Zurich, Switzerland. Electronic address: - - 2014
Atherosclerotic plaque rupture is the primary cause for myocardial infarction and stroke. During plaque progression macrophages and mast cells secrete matrix-degrading proteolytic enzymes, such as matrix metalloproteinases (MMPs). We studied levels of MMPs and tissue inhibitor of metalloproteinases-3 (TIMP-3) in relation to the characteristics of carotid plaques. We evaluated in ...
Harris Richard J RJ Department of Psychology and York Neuroimaging Centre, University of York, York YO10 5DD United - - 2014
Although different brain regions are widely considered to be involved in the recognition of facial identity and expression, it remains unclear how these regions process different properties of the visual image. Here, we ask how surface-based reflectance information and edge-based shape cues contribute to the perception and neural representation of ...
Jangi Mohini M David H. Koch Institute for Integrative Cancer - - 2014
The tight regulation of splicing networks is critical for organismal development. To maintain robust splicing patterns, many splicing factors autoregulate their expression through alternative splicing-coupled nonsense-mediated decay (AS-NMD). However, as negative autoregulation results in a self-limiting window of splicing factor expression, it is unknown how variations in steady-state protein levels ...
Tay Yvonne Y Cancer Research Institute, Beth Israel Deaconess Cancer Center, Department of Medicine and Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, - - 2014
In a recent issue of Nature, Kumar et al. demonstrate that the oncogenic potential of the Hmga2 gene is largely due to the ability of its transcript to operate as a competing endogenous RNA in a protein coding-independent manner. The Hmga2 mRNA decoys the let-7 microRNA family to regulate Tgfbr3 ...
Spedito Alessandro A Dipartimento di Biologia e Biotecnologie, Laboratorio di Biologia Cellulare e Neurobiologia, Università degli Studi di Pavia, Via Ferrata 1, Pavia, - - 2014
A new method is proposed which combines the high spatial resolution of transmission electron microscopy with information on the dynamics of transcription. Incorporation of two different RNA precursors was used to define a time transcription window on cultured cells treated with hypometabolizing peptides which are known to modulate transcription. This ...
Yan Wen-Jing WJ State Key Laboratory of Brain and Cognitive Science, Institute of Psychology, Chinese Academy of Sciences, Beijing, China ; University of Chinese Academy of Sciences, Beijing, - - 2014
A robust automatic micro-expression recognition system would have broad applications in national safety, police interrogation, and clinical diagnosis. Developing such a system requires high quality databases with sufficient training samples which are currently not available. We reviewed the previously developed micro-expression databases and built an improved one (CASME II), with ...
Seo Joonbae J Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, - - 2014
The dynamic process of pre-mRNA splicing is regulated by combinatorial control exerted by overlapping cis-elements that are unique to every exon and its flanking intronic sequences. Splicing cis-elements are usually 4-8-nucleotide-long linear motifs that furnish interaction sites for specific proteins. Secondary and higher-order RNA structures exert an additional layer of ...
Plass Mireya M Department of Biology, The Bioinformatics Centre, University of Copenhagen, Copenhagen, - - 2014
In higher eukaryotes, alternative splicing is usually regulated by protein factors, which bind to the pre-mRNA and affect the recognition of splicing signals. There is recent evidence that the secondary structure of the pre-mRNA may also play an important role in this process, either by facilitating or hindering the interaction ...
Patil Deepak P DP Center for RNA Biology, The Ohio State University, Columbus, OH, - - 2014
mRNA polyadenylation functions in nuclear export, translation, and stability. We describe an efficient protocol designed to assess poly(A) tail length that is based on 3' tailing by yeast poly(A) polymerase and product analysis to single-nucleotide resolution by capillary electrophoresis.
Speth Corinna C Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, - - 2014
Quantitative real-time PCR (qRT-PCR) is a common technique for mRNA quantification. Several methods have been developed in the past few years in order to adapt qRT-PCR also for small non-coding RNAs (sRNA). We here provide a simple and sensitive protocol that allows quantification of mRNAs, selected sRNAs, and long non-coding ...
Schlederer Michaela M Ludwig Boltzmann Institute for Cancer Research (LBI-CR), Vienna, - - 2014
In targeted therapy, patient tumors are analyzed for aberrant activations of core cancer pathways, monitored based on biomarker expression, to ensure efficient treatment. Thus, diagnosis and therapeutic decisions are often based on the status of biomarkers determined by immunohistochemistry in combination with other clinical parameters. Standard evaluation of cancer specimen ...
Machida Ryuji J RJ Biodiversity Research Centre, Academia Sinica, Nankang, Taipei, - - 2014
The 5' untranslated regions of mRNA play an important role in their translation. Here, we describe the development of four methods of profiling mRNA 5' ends using the Illumina sequencing platform; the first method utilizes SMART (Switching Mechanism At 5' end of RNA Transcript) technology, while the second involves replacing ...
Chang Yung-Heng YH Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China; Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of - - 2014
Morphogens are signaling molecules that regulate growth and patterning during development by forming a gradient and activating different target genes at different concentrations. The extracellular distribution of morphogens is tightly regulated, with the Drosophila morphogen Wingless (Wg) relying on Dally-like (Dlp) and transcytosis for its distribution. However, in the absence ...
Kopp Christina C Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany. - - 2014
The transition period in dairy cows (3 weeks prepartum until 3 weeks postpartum) is associated with substantial mobilization of energy stores, which is often associated with metabolic diseases. Nicotinic acid (NA) is an antilipolytic and lipid-lowering compound used to treat dyslipidaemia in humans, and it also reduces non-esterified fatty acids ...
Skene Peter J PJ Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, United - - 2014
RNA polymerase II (PolII) transcribes RNA within a chromatin context, with nucleosomes acting as barriers to transcription. Despite these barriers, transcription through chromatin in vivo is highly efficient, suggesting the existence of factors that overcome this obstacle. To increase the resolution obtained by standard chromatin immunoprecipitation, we developed a novel ...
Li Xiangqi X Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Shanghai 200031, - - 2013
Since the first miRNA was discovered in 1993, miRNAs have become a hotspot for biological research. In order to feed this demand, a robust method is required to detect miRNA gene expression. Development of a detection method is more difficult for miRNAs than for long RNAs, such as mRNA, owing ...
Baas Roy R 1Department of Molecular Cancer Research, University Medical Centre, Utrecht, The - - 2013
Posttranslational modifications of histones play an important role in the regulation of gene expression and chromatin structure in eukaryotes. The balance between chromatin factors depositing (writers) and removing (erasers) histone marks regulates the steady-state levels of chromatin modifications. Here we describe a novel microscopy-based screening method to identify proteins that ...
Schwartz Schraga S Broad Institute of MIT and Harvard, Cambridge, MA 02142, - - 2013
N(6)-methyladenosine (m(6)A) is the most ubiquitous mRNA base modification, but little is known about its precise location, temporal dynamics, and regulation. Here, we generated genomic maps of m(6)A sites in meiotic yeast transcripts at nearly single-nucleotide resolution, identifying 1,308 putatively methylated sites within 1,183 transcripts. We validated eight out of ...
Tebaldi T - - 2013
High-throughput technologies have led to an explosion of genomic data available for automated analysis. The consequent possibility to simultaneously sample multiple layers of variation along the gene expression flow requires computational methods integrating raw information from different '-omics'. It has been recently demonstrated that translational control is a widespread phenomenon, ...
Steijger Tamara T European Molecular Biology Laboratory, European Bioinformatics Institute, Cambridge, - - 2013
We evaluated 25 protocol variants of 14 independent computational methods for exon identification, transcript reconstruction and expression-level quantification from RNA-seq data. Our results show that most algorithms are able to identify discrete transcript components with high success rates but that assembly of complete isoform structures poses a major challenge even ...
Safikhani Zhaleh - - 2013
Recent advances in the sequencing technologies have provided a handful of RNA-seq datasets for transcriptome analysis. However, reconstruction of full-length isoforms and estimation of the expression level of transcripts with a low cost is a challenging task. We propose a novel de novo method named SSP that incorporate Interval Integer ...
Liu Nian - - 2013
N(6)-methyladenosine (m(6)A) is the most abundant modification in mammalian mRNA and long noncoding RNA (lncRNA). Recent discoveries of two m(6)A demethylases and cell-type and cell-state-dependent m(6)A patterns indicate that m(6)A modifications are highly dynamic and likely play important biological roles for RNA akin to DNA methylation or histone modification. Proposed ...
Khan Zia Z Department of Human Genetics, University of Chicago, Chicago, IL 60637, - - 2013
Changes in gene regulation have likely played an important role in the evolution of primates. Differences in messenger RNA (mRNA) expression levels across primates have often been documented; however, it is not yet known to what extent measurements of divergence in mRNA levels reflect divergence in protein expression levels, which ...
Behr Jonas - - 2013
High throughput sequencing of mRNA (RNA-Seq) has led to tremendous improvements in the detection of expressed genes and reconstruction of RNA transcripts. However, the extensive dynamic range of gene expression, technical limitations and biases, as well as the observed complexity of the transcriptional landscape pose profound computational challenges for transcriptome ...
1 2 3 4 5 6 7 8 9 10 >