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Shi Chun C Department of Anatomy, Guangzhou Medical University, Guangzhou, Guangdong, 510182, China, - - 2014
Mitochondrial dysfunction is a hallmark of amyloid β peptide (Aβ)-induced neuronal toxicity in Alzheimer's disease (AD). However, the underlying mechanism (s) of Aβ-induced mitochondrial dysfunction is still not fully understood. There is evidence that nuclear factor-κB (NF-κB) is involved in Aβ-induced neurotoxicity and is present in mitochondria. Using HT22 murine ...
Liang Jianfeng J Department of Neurosurgery, The University of Texas Medical School at Houston, Houston, Texas 77030, USA; Yale University School of Medicine, New Haven, CT 06520, USA; Department of Neurosurgery, China-Japan Friendship Hospital, Beijing 100029 - - 2014
Astrocytic excitatory amino acid transporters (EAATs) regulate extracellular glutamate concentrations and play a role in preventing neuroexcitotoxicity. Since δ-opioid receptor (DOR) is neuroprotective against excitotoxic injury, we asked if DOR activation upregulates EAAT expression and function. We measured mRNA and protein expression of EAAT1, EAAT2 and EAAT3 in cultured mouse ...
Kobori Takuro T Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kobe Gakuin - - 2014
 Altered expression of P-glycoprotein (P-gp), a drug efflux transporter expressed by brain capillary endothelial cells (BCECs), may contribute to the development of opioid analgesic tolerance, as demonstrated by cumulative evidence from research. However, the detailed mechanism by which chronic morphine treatment increases P-gp expression remains unexplained. Ezrin/radixin/moesin (ERM) are scaffold ...
Tang Shang-Jun SJ Department of General Surgery, Chen Xing Hai Hospital, Guangdong Medical College, Zhongshan, China; State Key Laboratory of Oncology in South China, Cancer Center of Sun Yat-Sen University, Guangzhou, China. Electronic address: - - 2014
The overexpression of ATP-binding cassette (ABC) transporters often leads to the development of multidrug resistance (MDR), which is the major factor contributing to the failure of chemotherapy. The objective of this study was to investigate the enhancement of CEP-33779, a small-molecule inhibitor of Janus kinase 2 (JAK2), on the efficacy ...
Jin Bo B Department of Biochemistry and Molecular Biology, Health Science Center, Peking University, 38 Xueyuan Road, Beijing, 100191, - - 2014
Cellular senescence is a stable state of proliferative arrest that provides a barrier against malignant transformation and contributes to the antitumor activity of certain chemotherapies. Unexpectedly, we found that the expression of proto-oncogene PIM-1, which can promote tumorigenesis, is induced at transcriptional level during senescence. Inhibition of PIM-1 alleviated both ...
Skop Vojtech V Center of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic; Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Prague, Czech - - 2014
Autophagy is essential for successful white adipocyte differentiation but the data regarding the timing and relevance of autophagy action during different phases of adipogenesis are limited. We subjected 3T3-L1 preadipocytes to a standard differentiation protocol and inhibited the autophagy within time-limited periods (days 0-2; 2-4; 4-6; 6-8) by asparagine or ...
Wilkins Heather M HM Department of Neurology University of Kansas Alzheimer's Disease - - 2014
Brain bioenergetic function declines in some neurodegenerative diseases, this may influence other pathologies, and administering bioenergetic intermediates could have therapeutic value. To test how one intermediate, oxaloacetate (OAA), affects brain bioenergetics, insulin signaling, inflammation, and neurogenesis we administered intraperitoneal OAA, 1-2 g/kg once per day for 1-2 weeks, to C57Bl/6 ...
Rack Paul G PG Departments of Chemical and Systems - - 2014
Hedgehog (Hh) pathway activation and Gli-dependent transcription play critical roles in embryonic patterning, tissue homeostasis, and tumorigenesis. By conducting a genome-scale cDNA overexpression screen, we have identified the Rho GAP family member Arhgap36 as a positive regulator of the Hh pathway in vitro and in vivo. Arhgap36 acts in a ...
Choi Min-Koo MK College of Pharmacy, Dankook University, 119 Dandaero, Cheonan, Chungnam, 330-714, - - 2014
The objective of this study was to investigate the contributions of a sodium-dependent concentrative nucleoside transporter (CNT) 1 and an equilibrative nucleoside transporter (ENT) 1 to ribavirin uptake in human hepatocytes. The initial studies in oocytes expressing CNT1 and ENT1 showed increases in ribavirin uptake, indicating that ribavirin was a ...
Chen Taiyu T National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, 430070, - - 2014
Engineering C4 photosynthetic metabolism into C3 crops is regarded as a major strategy to increase crop productivity, and clarification of the evolutionary processes of C4 photosynthesis can help the better use of this strategy. Here, Eleocharis baldwinii, a species in which C4 photosynthesis can be induced from a C3 -C4 ...
Ye Junyi - - 2014
Morphine has been widely used as a clinical anesthetic and analgesic. However, abuse of morphine might result in psychological and physiological dependence. Previous studies have indicated that memory mechanisms play critical roles in morphine dependence. Morphine dependence was established in mice utilizing place preference conditioning (CPP). We observed changes in ...
Shahbabian Karen K Département de Biochimie et Médecine Moléculaire, Université de Montréal, 2900 Boulevard Edouard-Montpetit, Montréal, QC, H3C 3J7, - - 2014
Messenger RNA (mRNA) localization is coupled to the translational repression of transcripts during their transport. It is still unknown if this coupling depends on physical interactions between translational control and mRNA localization machineries, and how these interactions are established at the molecular level. In yeast, localization of transcripts like ASH1 ...
Kawashima Ryoko L RL Department of Chemistry and Biochemistry, California State University Northridge, Northridge CA - - 2014
The ATP-binding cassette transporter A1 (ABCA1) mediates the efflux of excess cholesterol from foam cells to lipid-poor apolipoprotein A-I, in a process called reverse cholesterol transport. Lipoprotein lipase (LPL) is a lipolytic enzyme expressed by macrophages within atherosclerotic lesions. Lentivirus-mediated RNA interference was used to genetically knock-down (KD) the expression ...
Marguerat Samuel S Department of Genetics, Evolution & Environment and UCL Cancer Institute; University College London; London, - - 2014
The cooperation of transcriptional and post-transcriptional levels of control to shape gene regulation is only partially understood. Here we show that a combination of two simple and non-invasive genomic techniques, coupled with kinetic mathematical modeling, afford insight into the intricate dynamics of RNA regulation in response to oxidative stress in ...
Yen Hsiu-Chuan - - 2014
Abstract Endogenous coenzyme Q10 (CoQ10) is a lipid-soluble antioxidant and essential for the electron transport chain. We previously demonstrated that hydrogen peroxide enhanced CoQ10 levels, whereas disruption of mitochondrial membrane potential by a chemical uncoupler suppressed CoQ10 levels, in human 143B cells. In this study, we investigated how CoQ10 levels ...
Gu Wanjun W Research Center for Learning Sciences, Southeast University, Nanjing, Jiangsu 210096, China Wanjun.Gu@gmail.com akdongyi01@cau.ac.kr - - 2014
Recent studies have suggested that the secondary structure of the 5' untranslated region (5' UTR) of messenger RNA (mRNA) is important for microRNA (miRNA)-mediated gene regulation in humans. mRNAs that are targeted by miRNA tend to have a higher degree of local secondary structure in their 5' UTR; however, the ...
Pilyugin Maxim M Department of Gynecology and Obstetrics and Department of Medical Genetics and Laboratory Medicine, University Hospitals of Geneva, Geneva, Switzerland. Electronic address: - - 2014
Long non-coding RNAs (lncRNAs) are ubiquitously expressed RNA molecules of more than 200 nucleotides without substantial ORFs. LncRNAs could act as epigenetic regulators of gene expression affecting transcription, mRNA stability and transport, and translation, although, precise functions have been attributed to only few of them. Competing endogenous RNAs (ceRNAs) represent ...
Sharma Ankita A a National Bureau of Animal Genetic Resources , Karnal , Haryana , - - 2014
In the present study, expression level of various ATP-binding cassette (ABC) viz., ABCA1, ABCA7, ABCG1, ABCG2, and ABCG5; associated transcription factors viz., SREBF1, LXRα (NR1H3), PPARA, and Solute Carriers (SLC); or Glucose transporters (GLUT) viz., SLC2A1(GLUT1), SLC2A4 (GLUT4), SLC2A8 (GLUT8), and SLC2A12 (GLUT12) superfamily of transporters were compared across physiological ...
Vetere Gisella G Institute of Cell Biology and Neurobiology, National Research Council, Via del FossodiFiorano, 64/65, 00143, Roma, Italy; Fondazione Santa Lucia, Via del Fossodi Fiorano 64/65, 00143, Roma, - - 2014
Post-transcriptional gene regulation mediated by microRNAs (miRNAs) is implicated in memory formation; however, the function of miR-92 in this regulation is uncharacterized. The present study shows that training mice in contextual fear conditioning produces a transient increase in miR-92 levels in the hippocampus and decreases several miR-92 gene targets, including: ...
Kunze Annett A Novartis Pharma AG; Univsity of - - 2014
Organic anion transporting polypeptides (OATP) 1B1 and OATP1B3 are drug transporters mediating the active hepatic uptake of their substrates. Since they exhibit overlapping substrate specificities the contribution of each isoform to the net hepatic uptake needs to be considered when predicting drug-drug interactions. The relative contribution of OATP1B1 and OATP1B3-mediated ...
Takahashi Yoshinobu Y Department of Neurosurgery, Hokuto Hospital, Obihiro, Hokkaido 080-0039, - - 2014
Primary central nervous system lymphoma (PCNSL) is an aggressive form of non-Hodgkin lymphoma with a poor prognosis. [(18)F] 2-fluoro-2-deoxy-D-glucose (FDG) and L-(methyl-(11)C)-methionine (MET) are the most widely used tracers in oncological positron emission tomography studies for PCNSL and commonly identify hypermetabolic lesions through increased uptake of FDG and MET. However, ...
de Vreede Geert G Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, - - 2014
Scribble (Scrib) module proteins are major regulators of cell polarity, but how they influence membrane traffic is not known. Endocytosis is also a key regulator of polarity through roles that remain unclear. Here we link Scrib to a specific arm of the endocytic trafficking system. Drosophila mutants that block AP-2-dependent ...
Yan Lei L Laboratory of Animal Physiology and Biochemistry, Nanjing Agriculture University, 210095 Nanjing, - - 2014
We have tested the hypothesis that increased concentrate intake induces mRNA abundance alterations of genes involved in short-chain fatty acid (SCFA) absorption in the rumen epithelium of goats and these changes of mRNA abundance are probably related to ruminal SCFA concentration and ruminal pH. Goats (n = 12) were randomly ...
Wang Kun K Division of Cardiovascular Research, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, - - 2014
Long noncoding RNAs (lncRNAs) are emerging as new players in gene regulation, but whether lncRNAs operate in the processing of miRNA primary transcript is unclear. Also, whether lncRNAs are involved in the regulation of the mitochondrial network remains to be elucidated. Here, we report that a long noncoding RNA, named ...
Jian Qiang Q Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, - - 2014
The influence of UV irradiation on pigmentation is well established, but the molecular and cellular mechanisms controlling dendrite formation remain incompletely understood. MicroRNAs (miRNAs) are a class of small RNAs that participate in various cellular processes by suppressing the expression of target messenger RNAs. In this study, we investigated the ...
Tang Yujie Y 1] Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, California, USA. [2] Department of Neurosurgery, Stanford University School of Medicine, Stanford, California, - - 2014
Hedgehog signaling drives oncogenesis in several cancers, and strategies targeting this pathway have been developed, most notably through inhibition of Smoothened (SMO). However, resistance to Smoothened inhibitors occurs by genetic changes of Smoothened or other downstream Hedgehog components. Here we overcome these resistance mechanisms by modulating GLI transcription through inhibition ...
Liu Xuemei X Department of Gastroenterology, Hannover Medical School, Hannover, - - 2014
Slc26a9 is an anion transporter that is strongly expressed in the stomach and lung. Slc26a9 variants were recently found associated with a higher incidence of meconium ileus in cystic fibrosis (CF) infants, raising the question whether Slc26a9 is expressed in the intestine and what its functional role is. Slc26a9 messenger ...
Hu Hailong H State Key Laboratory of Urban Water Resource and Environment, School of Life Science and Technology, Harbin Institute of Technology, Harbin, - - 2014
Background: The chemical chaperone 4-phenylbutyric acid (4-PBA) has been shown to relieve endoplasmic reticulum (ER) stress. Therefore, it improves insulin sensitivity and promotes glucose metabolism in skeletal muscle. Glucose transporter type 4 (GLUT4), as a major glucose transporter protein, plays a central role in glucose metabolism. Until now, it has ...
Jahnel Jorg J Medical University of Graz; - - 2014
Objective: The enterohepatic circulation of bile acids (BA) critically depends on absorption of BA in the terminal ileum and colon, which can be affected by inflammatory bowel disease (IBD). Diarrhea in IBD is believed to result in part from BA malabsorption (BAM). We explored whether IBD alters mRNA expression of ...
Yang Zheng-Ting ZT State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, 200433, Shanghai, - - 2014
The accumulation of unfolded or misfolded proteins in endoplasmic reticulum (ER) triggers a well-conserved pathway called the unfolded protein response (UPR) in eukaryotic cells to mitigate the ER stress. Two signaling pathways,S2P-bZIP28 and IRE1-bZIP60, play important roles in transmitting the ER stress signals from the ER to the nucleus in ...
Yang Zhong-Fa ZF University of Massachusetts Medical School, Worcester, - - 2014
Mitochondria are membrane-bound cytoplasmic organelles that serve as the major source for ATP production in eukaryotic cells. GABP (also known as Nuclear Respiratory Factor 2) is a nuclear-encoded ets transcription factor that binds and activates mitochondrial genes which are required for electron transport and oxidative phosphorylation. We conditionally deleted Gabpa, ...
Soler Dellys M - - 2014
mRNA stability appears to play a key role in the ontogenic regulation of the apical sodium dependent bile acid transporter (ASBT). The RNA binding proteins, Hu antigen R (HuR) and Tristetraprolin (TTP), stabilize and destabilize ASBT mRNA, respectively. Potential HuR binding sites were assessed by sequence analysis in the context ...
Buchwalter Abigail L AL Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, 10010 N. Torrey Pines Road La Jolla, 92037 CA, - - 2014
The nuclear pore complex (NPC) plays a critical role in gene expression by mediating import of transcription regulators into the nucleus and export of RNA transcripts to the cytoplasm. Emerging evidence suggests that in addition to mediating transport, a subset of nucleoporins (Nups) engage in transcriptional activation and elongation at ...
Wang Xueqian X Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina - - 2014
Activation of nuclear factor E2-related factor-2 (Nrf2), a sensor of oxidative stress, is neuroprotective in animal models of cerebral ischemia, traumatic brain injury, subarachnoid hemorrhage, and spinal cord injury. We show here that Nrf2 activation with sulforaphane (SFN) in vivo or in vitro increases expression and transport activity of three ...
Inoue Yu Y Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Japan; - - 2014
Zinc is essential for the proper functioning of various enzymes and transcription factors, and its homeostasis is rigorously controlled by zinc transporters (SLC39/ZIP: importers, SLC/ZnT: exporters). Skin disease is commonly caused by a zinc deficiency. Dietary and inherited zinc deficiencies are known to cause alopecia and the development of vesicular ...
Ueno Akihisa A Department of Pathology, School of Medicine, Keio University, Tokyo, Japan; Department of Diagnostic Radiology, School of Medicine, Keio University, Tokyo, - - 2014
In the current era of emerging molecular targeted drugs, it is necessary to identify before treatment the specific subclass to which a tumor belongs. Gadoxetic acid is a liver-specific contrast agent that is preferentially taken up by hepatocytes. Therefore, gadoxetic acid-enhanced magnetic resonance imaging (EOB-MRI) should provide precise molecular information ...
Warsi Jamshed J Department of Physiology I, University of Tuebingen, Gmelinstr. 5, 72076, Tuebingen, - - 2014
Janus kinase 3 (JAK3) contributes to cytokine receptor signaling, confers cell survival and stimulates cell proliferation. The gain of function mutation JAK3(A572V) is found in acute megakaryoplastic leukemia. Replacement of ATP coordinating lysine by alanine yields inactive JAK3(K855A). Most recent observations revealed the capacity of JAK3 to regulate ion transport. This ...
Uchiyama-Kadokura Naoko N Graduate School of Life Science, Hokkaido University, Sapporo 060-0810, - - 2014
During mRNA translation, nascent peptides with certain specific sequences cause arrest of ribosomes that have synthesized themselves. In some cases, such ribosomal arrest is coupled with mRNA decay. In yeast, mRNA quality control systems have been shown to be involved in mRNA decay associated with ribosomal arrest. However, a link ...
Shao Yong Y University of - - 2014
Glucose is a major substrate for milk synthesis and is taken up from the blood by mammary epithelial cells (MECs) through facilitative glucose transporters (GLUTs). The expression levels of GLUT1 and GLUT8 are upregulated dramatically in the mammary gland from late pregnancy through early lactation stages. This study aimed to ...
Moitinho-Silva Lucas L Dept. of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Wuerzburg, Julius-von-Sachs Platz 3, 97082 Wuerzburg, - - 2014
Sponges are important components of marine benthic environments and are associated with microbial symbionts that carry out ecologically relevant functions. Stylissa carteri is an abundant, low-microbial abundance (LMA) species in the Red Sea. We aimed to achieve the functional and taxonomic characterization of the most actively expressed prokaryotic genes in ...
Poling Holly M HM Division of Gastroenterology, Hepatology, and Nutrition;Cincinnati Children's Hospital Medical Center;3333 Burnet Avenue, Cincinnati, Ohio, 45229, United States of America; Current location: Division of Pediatric General and Thoracic Surgery;Cincinnati Children's Hospital Medical Center;3333 Burnet Avenue, Cincinnati, Ohio, 45229, United States of America. Electronic address: - - 2014
Canalicular bile is secreted by hepatocytes and then passes through the intrahepatic bile ducts, comprised of cholangiocytes, to reach the extrahepatic biliary system. In addition to providing a conduit for bile to drain from the liver, cholangiocytes play an active role in modifying bile composition. Bile formation is the result ...
Daddaoua Abdelali A Department of Environmental Protection, CSIC-EEZ, C/Profesor Albareda 1, E-18008 Granada, Spain - - 2014
In the human pathogen Pseudomonas aeruginosa, the GltR regulator is required for glucose transport, whereas GtrS is a sensor kinase that plays a key role in mediating bacteria-host interaction and pathogen dissemination in the host. We show that GtrS and GltR form a two-component system that regulates the expression from ...
Hermesh Orit - - 2014
Localization of mRNAs and local translation are universal features in eukaryotes and contribute to cellular asymmetry and differentiation. In Saccharomyces cerevisiae, localization of mRNAs that encode membrane proteins requires the She protein machinery including the RNA-binding protein She2p as well as movement of the cortical endoplasmic reticulum (cER) to the ...
Kerchev Pavel Ivanov PI Department of Plant Systems Biology, VIB, Ghent University, B-9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, - - 2014
Mitochondria are crucial players in the signaling and metabolic homeostasis of the plant cell. The molecular components that orchestrate the underlying processes, however, are largely unknown. Using a chemical biology approach, we exploited the responsiveness of Arabidopsis UDP-glucosyltransferase-encoding UGT74E2 towards mitochondrial perturbation in order to look for novel mechanisms regulating ...
Michaud Morgane M Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 du Centre National de la Recherche Scientifique, Université de Strasbourg, 67084 Strasbourg Cedex, - - 2014
Intracellular targeting of mRNAs has recently emerged as a prevalent mechanism to control protein localization. For mitochondria, a cotranslational model of protein import is now proposed in parallel to the conventional posttranslational model, and mitochondrial targeting of mRNAs has been demonstrated in various organisms. Voltage-dependent anion channels (VDACs) are the ...
Mrozikiewicz Przemyslaw M - - 2014
Abstract There are a number of compounds that can modify the activity of ABC (ATP-binding cassette) and SLC (solute carrier) transporters in the blood-brain barrier (BBB). The aim of this study was to investigate the effect of natural and synthetic substances on the expression level of genes encoding transporters present ...
Chen Lei - - 2014
Bromodomain factor 1 (Bdf1p) is a transcriptional regulator. The absence of Bdf1p causes salt sensitivity with abnormal nucleus and mitochondrial dysfunction. In this study, we reported that the salt sensitivity, mitochondrial dysfunction, and nuclear instability of bdf1Δ mutant were suppressed by HDA1 deletion or MEF1 overexpression. Hda1p overexpression inhibited the ...
Tashiro Natsuka N Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, - - 2014
The freshwater planarian Dugesia japonica has a simple central nervous system (CNS) and can regenerate complete organs, even a functional brain. Recent studies demonstrated that there is a great variety of neuronal-related genes, specifically expressed in several domains of the planarian brain. We identified a planarian dat gene, named it ...
Boehm-Cagan Anat A Department of the Neurobiology, The George S. Wise Faculty of Life Sciences, The Sagol School of Neuroscience, Tel Aviv University, Tel Aviv 69978, - - 2014
Apolipoprotein E4 (apoE4), the most prevalent genetic risk factor for Alzheimer's disease (AD), is less lipidated than its corresponding AD-benign form, apoE3, and it has been suggested that the pathological effects of apoE4 are mediated by lipid-related mechanisms. ATP-binding cassette transporters A1 and G1 (ABCA1 and ABCG1, respectively) are the ...
Kumafuji Masayuki M Laboratory of Gene Regulation, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, - - 2014
The Mediator complex (Mediator) is conserved among eukaryotes and is comprised of head, middle, tail and CDK/cyclin modules. The head module has received the most attention because its interaction with RNA polymerase II (Pol II) and the general transcription factors TFIIH and TBP facilitates phosphorylation of the carboxy-terminal domain (CTD) ...
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