Search Results
Results 1 - 50 of 1671
1 2 3 4 5 6 7 8 9 10 >
Zeng Xian-Cheng - - 2014
Wnt/beta-catenin signaling pathway plays important roles in human cancer progression. Better understanding the mechanism underlying regulation of Wnt/beta-catenin signaling pathway might provide novel therapeutic targets for cancer treatment. miR-610 expression levels in hepatocellular carcinoma (HCC) cell lines, HCC tissues and 76 archived HCC specimens were determined using real-time PCR. Cell ...
Preidl Raimund H M RH Department of Oral and Maxillofacial Surgery, University of Erlangen- Nürnberg, Glückstraße 11, 91054, Erlangen, Germany, - - 2014
Microvascular free tissue transfer is a standard method in head and neck reconstructive surgery. However, previous radiotherapy of the operative region is associated with an increased incidence in postoperative flap-related complications and complete flap loss. As transforming growth factor beta (TGF-β) 1 and galectin-3 are well known markers in the ...
Poletto Ana Cláudia AC Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Sao Paulo, - - 2014
Oleic (OA) and linoleic (LA) fatty acids may be important regulators of Slc2a4 gene (GLUT4 protein) in skeletal muscle, thus participating in insulin resistance. We investigated the effect of OA and LA on the Slc2a4/GLUT4 expression in L6 muscle cells; as well as potential transcriptional regulators. OA and LA (50-400 ...
Pinto Debora P DP Programa de Pós-graduação em Biologia de Ambientes Aquáticos Continentais, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS 96203-900, - - 2014
Cytochrome P450 1A (CYP1A) expression in fish is used as a biomarker of exposure to organic contaminants, such PAHs, PCBs and dioxins, in the aquatic environment. South American guppy fish Jenynsia multidentata were exposed to the prototypical aryl hydrocarbon receptor (AHR) agonist beta-naphthoflavone (BNF; 1μM) and the fins were biopsied ...
Guan Zhong - - 2014
Aberrant activation of the Wnt/beta-catenin signaling pathway is an important factor in the development of nasopharyngeal carcinoma (NPC). Previous studies have demonstrated that the developmental gene sex-determining region Y (SRY)-box 1 (SOX1) inhibits cervical and liver tumorigenesis by interfering with the Wnt/-catenin signaling pathway. However, the role of SOX1 in ...
Manyes Lara - - 2014
Our prior characterization of RasGrf1 deficient mice uncovered significant defects in pancreatic islet count and size as well as beta cell development and signaling function, raising question about the mechanisms linking RasGrf1 to the generation of those "pancreatic" phenotypes. Here, we compared the transcriptional profile of highly purified pancreatic islets ...
Li Feixue - - 2014
CXADR-like membrane protein (CLMP) is a novel cell-cell adhesion molecule. The present study investigated the spatiotemporal expression pattern of CLMP and its regulation in the rat ovary during the periovulatory period. Real-time polymerase chain reaction analysis revealed that Clmp mRNA was rapidly stimulated in intact ovaries by 4h after human ...
Chung Yi Y Department of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital, Taipei, - - 2014
Cyclosporine-A (CsA)-induced gingival overgrowth may arise from an alteration in stoma matrix homeostasis. Sonic hedgehog (Shh) plays a key role during embryogenic development and fibrotic progression, and may be involved in CsA-altered gingival matrix homeostasis. Using the reverse transcription-polymerase chain reaction and Western blot analysis, we investigated the mRNA and ...
Jingushi Kentaro K Laboratory of Molecular and Cellular Physiology, Graduate School of Pharmaceutical Sciences, Osaka University - - 2014
Renal cell carcinoma (RCC) is the most common neoplasm of the adult kidney, and clear cell RCC (ccRCC) represents its most common histological subtype. To identify a therapeutic target for ccRCC, microRNA (miRNA) expression signatures from ccRCC clinical specimens were analyzed. miRNA microarray and real-time PCR analyses revealed that miR-629 ...
Rosano Laura L Molecular Pathology lab., Regina Elena Cancer - - 2014
The high mortality of epithelial ovarian cancer (EOC) is mainly caused by resistance to the available therapies. In EOC, the endothelin-1 (ET-1, EDN1)-endothelin A receptor (ETAR, EDNRA) signaling axis regulates the epithelial-mesenchymal transition (EMT) and a chemoresistant phenotype. However, there is a paucity of knowledge about how ET-1 mediates drug ...
Choi Igseo - - 2014
Transcriptome analysis of porcine whole blood has several applications, which include deciphering genetic mechanisms for host responses to viral infection and vaccination. The abundance of alpha- and beta-globin transcripts in blood, however, impedes the ability to cost-effectively detect transcripts of low abundance. Although protocols exist for reduction of globin transcripts ...
Zhou Chengwen C Department of Neurology, Vanderbilt University School of Medicine, Nashville, TN 37232 - - 2014
We previously demonstrated that heterozygous deletion of Gabra1, the mouse homolog of the human absence epilepsy gene that encodes the GABAA receptor (GABAAR) α1 subunit, causes absence seizures. We showed that cortex partially compensates for this deletion by increasing the cell surface expression of residual α1 subunit and by increasing ...
Berghold Veronika M VM Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Harrachgasse 21/VII, 8010, Graz, - - 2014
A crucial factor for effective villous trophoblast fusion in the human placenta is the transient deregulation of plasma membrane phospholipid asymmetry leading to externalization of phosphatidylserine to the outer membrane leaflet. Screening of scramblase family members implicated in the collapse of phospholipid asymmetry revealed that phospholipid scramblase 1 (PLSCR1) is ...
Kuwabara Yoshimitsu Y Y Kuwabara, Obstetrics and Gynecology, Nippon Medical School, Tokyo, 113-8602, Japan - - 2014
Osteopontin (OPN), a secreted glycoprotein, has multiple physiological functions. This study investigated OPN regulation and roles in the mouse ovary during periovulatory stages. Immature female mice were treated with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) to simulate follicle maturation and ovulation. In situ hybridization and real-time ...
Sayasith Khampoun - - 2014
The LH preovulatory surge stimulates several signal pathways essential for ovulation, and the regulator of G protein signaling-2 (RGS2) protein is thought to be involved in this process. The objectives of this study were to characterize the regulation of RGS2 transcripts in equine and bovine follicles prior to ovulation, and ...
Guo Feng F Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University, Nanjing 210095, PR - - 2014
Fat mass and obesity-associated (FTO) gene was found to be associated with energy homeostasis in mammals, yet the function of chicken FTO is less clear. In this study, chicken embryo fibroblast cells (DF-1) were transiently transfected to over-express (FTO(+)) or to knockdown (FTO(-)) the chicken FTO gene and were used ...
Wang Rui R R Wang, Department of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases,Shanghai Key Laboratory for Endocrine Tumors and E-Institutes of Shanghai Universities, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, - - 2014
WNT/beta-catenin signalling is involved in regulating adipogenesis, and its dysregulation occurs in obesity. Secreted frizzled-related protein (SFRP) 5 is a WNT protein inhibitor; however, its role in adipogenesis and obesity is controversial. In the present study, we observed that SFRP5 mRNA levels were increased in the fat tissues of obese ...
Rajakumar Anbazhagan A Laboratory of Molecular Endocrinology and Reproductive Biology, Department of Animal Biology, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, - - 2014
In teleosts, the levels of steroids during critical period of sex differentiation are critical for gonadogenesis. Hence, steroidogenesis and expression of steroidogenic enzyme genes are very critical for gonadal development and function. In this regard, 17β-HSDs are important as they are involved in both 17β-estradiol (E2) and testosterone (T) biosynthesis. ...
Bruun Christine C Hagedorn Research Institute, Gentofte, - - 2014
Impairment of beta cell mass and function is evident in both type 1 and type 2 diabetes. In healthy physiological conditions pancreatic beta cells adapt to the body's increasing insulin requirements by proliferation and improved function. We hypothesised that during the development of diabetes, there is an increase in the ...
Patel Rekha S RS University of South Florida, United - - 2014
Obesity is characterized by adipocyte hyperplasia and hypertrophy. We previously showed that PKCδ expression is dysregulated in obesity (1). Using 3T3L1 pre-adipocytes, we studied adipogenesis in vitro and showed that expression of PKCδ splice variants, PKCδI and PKCδII, have different expression patterns during adipogenesis (2). Here, we evaluated the role ...
Villate Olatz O ULB Center for Diabetes Research, Medical Faculty, Université Libre de Bruxelles, Brussels (ULB) B-1070, Belgium - - 2014
Alternative splicing (AS) is a fundamental mechanism for the regulation of gene expression. It affects more than 90% of human genes but its role in the regulation of pancreatic beta cells, the producers of insulin, remains unknown. Our recently published data indicated that the 'neuron-specific' Nova1 splicing factor is expressed ...
Shi J J Department of Urology, Tangshan Gongren Hospital, Hebei Medical University, No. 27, Wenhua Road, Lubei District, Tangshan, 063000, - - 2014
TGF-beta can induce G1 arrest via many mechanisms including up-regulating p21, p27, and Rb. However, as the member of Rb family, whether RBL2 is induced by TGF-beta treatment remains exclusive. The expression of RBL2 and miR-93 after TGF-beta treatment was determined by quantitative real-time PCR and western blot. The growth ...
Nakamoto Mitsuhiro M Department of Pathology and Oncology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555, - - 2014
Pancreatic ductal adenocarcinoma (PDA) is one of the most lethal human malignancies and is associated with a variety of molecular abnormalities. Although WNT signaling through its canonical/non-canonical pathways is one of the major factors involved in oncogenesis or progression of PDA, the prognostic significance of WNT signaling still remains poorly ...
Gindner Antonia A Department of Microbiology and Archaea Center, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, - - 2014
Microbes are organisms which are well adapted to their habitat. Their survival depends on the regulation of gene expression levels in response to environmental signals. The most important step in regulation of gene expression takes place at the transcriptional level. This regulation is intriguing in Archaea because the eu-karyotic-like transcription ...
Seldin Marcus M MM Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, - - 2014
Mammalian hibernation elicits profound changes in whole-body physiology. The liver-derived hibernation protein (HP) complex, consisting of HP-20, HP-25 and HP-27, was shown to oscillate circannually, and this oscillation in the central nervous system (CNS) was suggested to play a role in hibernation. The HP complex has been found in hibernating ...
Chaudhary Neelam N Laboratory of Cell Death & Cell Survival, Centre for DNA Fingerprinting and Diagnostics (CDFD), Nampally, Hyderabad 500001, - - 2014
The balance between transcription factor p73 and its functionally opposing N-terminal truncated ΔNp73 isoform is critical for cell survival but the precise mechanism that regulates their levels is not clear. In our study, we identified WWP2, an E3 ligase as a novel p73 associated protein that ubiquitinates and degrades p73. ...
Eusebio M M Department of Laboratory Diagnostics, Medical University of Lodz, - - 2014
There is an increasing body of evidence that alterations of regulatory T (Treg) cell numbers and functions lead to immune disorders. Accordingly, understanding the regulatory mechanisms that maintain peripheral regulatory T (Treg) cell homeostasis is key to the development of effective targeted biologic therapies. We previously demonstrated the effects of ...
Kim J J Division of Life Sciences, Korea University, Seoul 136-701, - - 2014
Mesenchymal stem cells (MSCs), also known as multipotent stromal cells, are used in clinical trials. However, the use of MSCs for medical treatment of patients poses a potential problem due to the possibility of transdifferentiation into unwanted tissues. Disruption of the balance during MSC differentiation leads to obesity, skeletal fragility, ...
Tinoco Ana B AB Departamento de Fisiología (Fisiología Animal II). Facultad de Biología. Universidad Complutense de Madrid. 28040 Madrid, - - 2014
Daily rhythms of feeding regulators are currently arousing research interest due to the relevance of the temporal harmony of endocrine regulators for growth and welfare in vertebrates. However, it is unknown the leptin circadian pattern in fish. The aim of this study is to investigate if leptin (gLep-aI and gLep-aII) ...
Rodriguez-Pacheco Francisca F Unidad de Gestion Clinica de Endocrinogía y Nutrición, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain; CIBERDEM, Instituto de Salud Carlos III, Malaga, - - 2014
The effects that fatty acids (FAs) exert on G protein-coupled receptor-120 (GPR120) levels, a receptor for FAs, are still unknown. We analyzed the association between GPR120 and obesity, and the FA effects on its expression. GPR120 levels were analyzed in visceral adipose tissue (VAT) from nonobese and morbidly obese subject. ...
Chan Hsiao Chang HC Epithelial Cell Biology Research Center, Key Laboratory for Regenerative Medicine of Ministry of Education of China, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong - - 2014
While microRNAs (miRNAs) have been recognized as one of the important epigenetic mechanisms that regulate gene expression in response to changes in environments, the links between environmental cues to alternation in miRNAs remain largely unknown. Localized to the cell membrane and recognized as an anion channel, the cystic fibrosis transmembrane ...
Jofré Matías R - - 2014
SPI-18 is a pathogenicity island found in some Salmonella enterica serovars, including S. Typhi. SPI-18 harbors two ORFs organized into an operon, hlyE and taiA genes, both implicated in virulence. Regarding the hlyE regulation in S. Typhi, it has been reported that RpoS participates as transcriptional up-regulator under low pH ...
Trombley Susanne S Department of Organismal Biology, Comparative Physiology, Evolutionary Biology Centre, Uppsala University, SE-752 36 Uppsala, - - 2014
In mammals, leptin acts as an adiposity signal and is a crucial link between nutritional status and the reproductive axis. So far the link between leptin and energy balance during sexual maturation in teleosts has been poorly investigated. In this study, seasonal gene expression changes in two leptin genes (lepa1 ...
Sharma Bal Krishan BK Department of Biochemistry and Molecular Biology, Molecular Oncology and Biomarkers Program, Georgia Regents University Cancer Center, Georgia Regents University, Augusta, GA - - 2014
Brown adipose tissue (BAT) is specialized for energy expenditure, a process called adaptive thermogenesis. PET-CT scans recently demonstrated the existence of metabolically active BAT in adult humans, which revitalized our interest in BAT. Increasing the amount and/or activity of BAT holds tremendous promise for the treatment of obesity and its ...
Wang Yufeng Y Laboratory of Livestock physiology, Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, 3001 Leuven, - - 2014
AMP-activated protein kinase (AMPK) is an evolutionary conserved cellular energy sensor, which plays a pivotal role in mammalian energy homeostasis. The present study was aimed to explore the possible involvement of hypothalamic AMPK in feed intake regulation of broiler chickens. Hence, diets with 0, 0.05% or 0.1% α-lipoic-acid (α-LA), a ...
Liu Ying - - 2014
Glycyrrhiza uralensis Fisch. ex DC is widely used in traditional Chinese medicine (TCM). Among its various active components, glycyrrhizic acid is believed to be the marker component. Squalene synthase (SQS) and beta-amyrin synthase (beta-AS) are key enzymes in the biosynthetic pathway of glycyrrhizic acid in G uralensis. To reveal the ...
Simons A M AM Department of Biology, Carleton University, Ottawa, ON, - - 2014
Adaptive phenotypic plasticity evolves when cues reliably predict fitness consequences of life-history decisions, whereas bet hedging evolves when environments are unpredictable. These modes of response should be jointly expressed, because environmental variance is composed of both predictable and unpredictable components. However, little attention has been paid to the joint expression ...
Xia T T Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, the Graduate School of the Chinese Academy of Sciences, Chinese Academy of - - 2014
Background:In the central nervous system (CNS), thyrotropin-releasing hormone (TRH) plays an important role in regulating energy balance. We previously showed that dietary deprivation of leucine in mice increases energy expenditure through CNS-dependent regulation. However, the involvement of central TRH in this regulation has not been reported.Methods:Male C57J/B6 mice were maintained ...
Hiong Kum C KC Natural Sciences and Science Education, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore, 637616, Republic of - - 2014
This study aimed to clone and sequence Na (+) / K (+)-ATPase (nka) α-subunit isoforms from, and to determine their mRNA expression levels and protein abundance in the brain of the African lungfish, Protopterus annectens during the induction, maintenance and arousal phases of aestivation in air. We obtained the full ...
Voss Cristina - - 2014
Many studies of the eukaryotic transcription mechanism and its regulation rely on in vitro assays. Conventional RNA polymerase II transcription assays are based on radioactive labelling of the newly synthesized RNA. Due to the inefficient in vitro transcription, the detection of the RNA involving purification and gel electrophoresis is laborious ...
Fishbach Ayelet A Booth School of Business, University of Chicago, Chicago, IL 60637. ayelet.fishbach@chicagobooth.edu - - 2014
I present evidence in favor of an overarching motivational self: a mental function that regulates expression of multiple goals. Goals often conflict with each other, and the role of a motivational self is to consciously or unconsciously prioritize pursuit of these goals. When observing inconsistency in expression of goals, it ...
Choi Young Jae - - 2014
Leptin plays an important role in energy homeostasis and reproductive function in fish, especially in reproduction. Migrating fish, such as salmonoids, are affected by external environmental factors, and salinity changes are a particularly important influence on spawning migrations. The aim of this study was to test whether changes in salinity ...
McEachern Lori A LA Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, - - 2014
Antisense transcription is prevalent in mammalian genomes, yet the function of many antisense transcripts remains elusive. We have previously shown that the fibroblast growth factor 2 (FGF2) gene is regulated endogenously by an overlapping antisense gene called Nudix-type motif 6 (NUDT6). However, the molecular mechanisms that determine the balance of ...
Nteeba Jackson - - 2014
Insulin, elevated during obesity, regulates xenobiotic biotransformation enzymes, potentially through phosphatidylinositol 3-kinase (PI3K) signaling, in extra-ovarian tissues. PI3K regulates oocyte viability, follicular activation and ovarian chemical biotransformation. 7,12-dimethylbenz[a]anthracene (DMBA), a carcinogen and ovotoxicant, destroys all stage follicles, leading to premature ovarian failure. Obesity has been reported to promote DMBA-induced tumors, ...
Kim Youn Jung YJ Kyung Hee University College of Nursing Science, Seoul, - - 2014
To assess innate and humoral immune responses in middle ear effusion of obese pediatric patients with otitis media with effusion (OME). We evaluated 219 children with OME, of whom 21 were obese and 198 were non-obese. We compared the expression in middle ear effusion of mRNAs encoding toll-like receptors (TLR) ...
Perkins S D SD Department of Animal Sciences, Auburn University, Auburn, AL - - 2014
Mechanisms underlying variation in residual feed intake (RFI), a heritable feed efficiency measure, are poorly understood while the relationship between RFI and meat quality is uncertain. To address these issues, two divergent cohorts consisting of High (HRFI) and Low (LRFI) RFI individuals were created by assessing RFI in forty-eight Angus-sired ...
Wang Weimin W College of Animal Science and Technology, Gansu Agriculture University, Lanzhou, 730070, - - 2014
Ghrelin, an endogenous ligand for growth hormone secretagogue receptor, plays an important role in stimulating hormone secretion, development of gastrointestinal tract, food intake and regulating energy balance of animals. In this study we isolated the cDNA sequence of ovine Ghrelin from the abomasums of 7-day-aged lambs. Real-time PCR was used ...
Viesti A Collares Renata R Department of Surgery and Anatomy of the Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirao Preto, SP, - - 2014
Obesity is a multifactorial disease, with epigenetic alterations. Have been described modifications in the expression of some microRNAs, and some proteins related to obesity. The objective was to determine and correlate, in obese patients, the gene expression of LEP, LEPR, IGF1, IL10 and of miR-27a, miR-27b, miR-143 and miR-145. RNA ...
Huang Zan Z 1] MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, Nanjing, Jiangsu 210061, China - - 2014
Cell growth is tightly coupled with mitochondrial biogenesis in order to maintain energy and organelle homeostasis. Receptor tyrosine kinase Kit and its ligand, stem cell factor (SCF), play a critical role in the growth and survival of multiple cell lineages. Here we report that the expression of SCF and Kit ...
Tao Wensi W Sylvester Comprehensive Cancer Center, and Department of Cell Biology, Graduate Program in Molecular Cell and Developmental Biology, University of Miami Miller School of Medicine, Miami, Florida, United States of - - 2014
Disabled-2 (Dab2) is a widely expressed endocytic adaptor that was first isolated as a 96 KDa phospho-protein, p96, involved in MAPK signal transduction. Dab2 expression is lost in several cancer types including breast cancer, and Dab2 is thought to have a tumor suppressor function. In mammary epithelia, Dab2 was induced ...
1 2 3 4 5 6 7 8 9 10 >