Search Results
Results 1 - 50 of 2448
1 2 3 4 5 6 7 8 9 10 >
Kraus Petra P Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY, 13699, - - 2015
With the advent of BAC recombineering techniques, transcriptional promoter and enhancer isolation studies have become much more feasible in zebrafish than in mouse given the easy access to large numbers of fertilized zebrafish eggs and offspring in general, the easy to follow ex-utero development of zebrafish, an overall less skill ...
Zhan Rongrong R State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, - - 2014
Inulin fructotransferase (IFTase) catalyses inulin conversion to difructose anhydride (DFA III), which is a natural low-calorie sweetener. Although heterologous expression of IFTase was achieved in Escherichia coli, the extracellular enzyme activity was very low which limited the commercialization of IFTase. Active IFTase of about 43 kDa molecular mass of subunit ...
Bandopadhyay Manikankana - - 2014
Hepatitis B Virus (HBV) X protein (HBx) is known to be involved in the initiation and progression of hepatocellular carcinoma (HCC) through modulation of host gene response. Alterations in miRNA expressions are frequently noted in HCC. This study is aimed to examine the role of HBx protein in the modulation ...
Almeida Juliana F - - 2014
BackgroundFibronectin Binding Protein A (FnBPA) is an invasin from Staphylococcus aureus that allows this pathogen to internalize into eukaryote cells. It was previously demonstrated that recombinant Lactococcus lactis expressing FnBPA were invasive and able to transfer a plasmid to eukaryotic cells in vitro and in vivo. In this study, the ...
Larentis Ariane Leites - - 2014
Leptospirosis is a zoonose that is increasingly endemic in built-up areas, especially where there are communities living in precarious housing with poor or non-existent sanitation infrastructure. Leptospirosis can kill, for its symptoms are easily confused with those of other diseases. As such, a rapid diagnosis is required so it can ...
Sun Piyun P Department of Neurology, The Fourth Affiliated Hospital, Harbin Medical University, No. 37 Jiyuan street, Harbin, Heilongjiang, 150001, - - 2014
Matrix metalloproteinase 3 (MMP-3) is implicated in the pathogenesis and progression of glioma. However, whether MMP-3 participates in the regulation of metastasis and its mechanisms in glioma is mostly unknown. In the present study, glioma cells were stably transfected with Bmi-1 small interfering RNA (siRNA) to knockdown off Bmi-1 or ...
Gajewska Alina A A Gajewska, Department of Neuroendocrinology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Jablonna n. Warsaw, 05-110, Poland - - 2014
EGR1 and PITX1 are transcription factors required for gonadotroph cell Lhb promoter activation. To determine changes in Egr1 and Pitx1 mRNA levels in central and peripheral pituitary stimulations, an in vivo model based on intracerebroventricular (i.c.v.) pulsatile (1 pulse/0.5 h over 2 h) GnRH agonist (1.5 nM buserelin) or antagonist ...
Beer Lucian - - 2014
High dose ionizing radiation (IR) induces potent toxic cell effects mediated by either direct DNA damage or the production of reactive oxygen species (ROS). IR-induced modulations in multiple biological processes have been proposed to be partly regulated by radiosensitive microRNA (miRNA). In order to gain new insights into the role ...
van der Lelij Petra P IMP Research Institute of Molecular Pathology, Vienna, - - 2014
Although splicing is essential for the expression of most eukaryotic genes, inactivation of splicing factors causes specific defects in mitosis. The molecular cause of this defect is unknown. Here, we show that the spliceosome subunits SNW1 and PRPF8 are essential for sister chromatid cohesion in human cells. A transcriptome-wide analysis ...
Jeong Sang Hoon SH Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology and Division of Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, - - 2014
The early growth response (Egr)-1 is a transcriptional factor which plays an important role in the regulation of cell growth, differentiation, cell survival and immune responses. Emerging evidences including our data demonstrate that the Egr-1 expression is up-regulated in the psoriatic skin lesions. The purpose of this study was to ...
Andrie Jennifer J University of - - 2014
Gene expression levels are determined by the balance between rates of mRNA transcription and decay, and genetic variation in either of these processes can result in heritable differences in transcript abundance. Although the genetics of gene expression has been the subject of intense interest, the contribution of heritable variation in ...
Wilkinson Adam C AC Cambridge Institute for Medical Research and Wellcome Trust-MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, - - 2014
Transcription factors (TFs) act within wider regulatory networks to control cell identity and fate. Numerous TFs, including Scl (Tal1) and PU.1 (Spi1), are known regulators of developmental and adult haematopoiesis, but how they act within wider TF networks is still poorly understood. Transcription activator-like effectors (TALEs) are a novel class ...
Murayama Tsugiya T Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Hokuriku University, Ho-3 Kanagawa-machi, Kanazawa 920-1181, Japan. Electronic address: - - 2014
We previously revealed that human cytomegalovirus (HCMV) infection can cause aberrant expression of the chemokine IL-8/CXCL8. We first examined the effects of HCMV infection on the expression of another chemokine, CCL2. HCMV infection induced CCL2 expression at the mRNA and protein levels in human embryonic lung fibroblasts cells (HEL). Moreover, ...
Kim Bo-Mi BM Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 440-746, South - - 2014
Superoxide dismutases (SODs) are important antioxidant enzymes whose expression levels are often used as biomarkers for oxidative stress. To investigate the biomarker potential of the monogonont rotifer Brachionus koreanus SOD genes, the full-length Cu/Zn-SOD (Bk-Cu/Zn-SOD) and Mn-SOD (Bk-Mn-SOD) genes were cloned from genomic DNA and characterized. All amino acid residues ...
Shang Xueying X College of Animal Science and Technology, Northwest A&F University, Yangling 712100, - - 2014
Melanoma differentiation-associated gene 5 (MDA5) plays a crucial role in recognizing intracellular viral infection, activating the interferon regulatory factor pathways as well as inducing antiviral response. While the antiviral regulatory mechanism of MDA5 remains unclear. In the present study, CiMDA5 (Ctenopharyngodon idella MDA5) against grass carp reovirus (GCRV) would be ...
Lavan Nadine N a Department of Psychology, Royal Holloway , University of London , Egham , - - 2014
It is well established that categorising the emotional content of facial expressions may differ depending on contextual information. Whether this malleability is observed in the auditory domain and in genuine emotion expressions is poorly explored. We examined the perception of authentic laughter and crying in the context of happy, neutral ...
Dodia Raksha - - 2014
Subunit 5 of Saccharomyces cerevisiae CcO is essential for assembly and has two isoforms, 5A and 5B. 5A is expressed under normoxic conditions while 5B is expressed at very low oxygen tensions. As a consequence, COX5A-deleted strains (Δcox5A) have no or only low levels of CcO under normoxic conditions rendering ...
Vogt Stefanie L SL Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G - - 2014
The Escherichia coli genome encodes approximately 30 two-component systems that are required for sensing and responding to a variety of environmental and physiological cues. Recent studies have revealed numerous regulatory connections between two-component systems and small regulatory RNAs (sRNAs), which post-transcriptionally regulate gene expression by base-pairing with target mRNAs. In ...
Sakurai Haruna H Department of Life Science, Setsunan University, 17-8 Ikeda-Nakamachi, Neyagawa, Osaka 572-8508, - - 2014
RecQ5, a member of the conserved RecQ DNA helicase family, is required for the maintenance of genome stability. The human RECQL5 gene is expressed ubiquitously in almost all tissues, with strong expression in the testes (Shimamoto et al., 2000). However, it remains to be elucidated in which cells RecQ5 is ...
Kumar Dilip D Department of Plant Sciences, University of Hyderabad, Hyderabad, - - 2014
We have identified a transcript derived fragment (TDF) corresponding to SGT1 in a study of differential gene expression on the resistant wild peanut, Arachis diogoi, upon challenge from the late leaf spot pathogen, Phaeoisariopsis personata, and cloned its full-length cDNA followed by subsequent validation through q-PCR. Sodium nitroprusside, salicylic acid, ...
Onishi Chie C Shimane University School of Medicine, - - 2014
Internal tandem duplication mutations in the Flt3 gene (ITD-Flt3) enhance cell migration toward the chemokine CXCL12, which is highly expressed in the therapy-protective bone marrow niche, providing a potential mechanism underlying the poor prognosis of ITD-Flt3(+) AML. We aimed to investigate the mechanisms linking ITD-Flt3 to increased cell migration toward ...
Wegner Waja W University Medical Center - - 2014
The human organic anion transporter 1 (OAT1) is crucial for the excretion of organic anions in renal proximal tubular cells, and has been classified as a clinically relevant transporter in the kidneys. Our previous study indicated that renal male-predominant expression of rat Oat1 and Oat3 appears to be regulated by ...
Snellenberg Suzanne S Department of Pathology, Unit of Molecular Pathology, VU University Medical Center, Amsterdam, The - - 2014
Promoter methylation of the transcription factor PRDM14 (PRDI-BF1 and RIZ domain containing 14) represents a highly frequent event in human papillomavirus (HPV)-induced cervical cancers and cancer precursor lesions. Here, we aimed to assess the functional consequences of PRDM14 promoter methylation in HPV-induced carcinogenesis. PRDM14 promoter methylation, expression and consequences of ...
Zhao Guangfeng - - 2014
Primary ovarian insufficiency (POI) is a serious reproductive dysfunction with the follicle pool reduced and depleted. Abnormal apoptosis of ovarian granulosa cells (GCs) is believed to result in follicle loss. Progesterone receptor membrane component (PGRMC) 1, critical for the GC survival, was reported to reduce in POI patients, but the ...
Cruz Cynthia Dela CD Division of Human Reproduction, Department of Obstetrics and Gynecology, Federal University of Minas Gerais, Belo Horizonte, - - 2014
Nodal is a growth factor of the transforming growth factor β superfamily that is expressed in high turnover tissues, such as the human endometrium, and in several malignancies. The effects of Nodal are modulated by the coreceptor Cripto and mediated by SMAD proteins. This study evaluated the gene and protein ...
Wang Chang-Quan CQ Department of Plant Biology, University of California, Davis, California - - 2014
The timely transition of vegetative to reproductive development is coordinated through quantitative regulation of floral pathway genes in response to physiological and environmental cues. Here, we show that the circadian-controlled expression of the Arabidopsis thaliana floral transition regulators FLOWERING LOCUS T (FT) and CONSTANS (CO) is antiphasic to that of ...
Kedmi Adi A The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, - - 2014
Transcription of protein-coding genes is highly dependent on the RNA polymerase II core promoter. Core promoters, generally defined as the regions that direct transcription initiation, consist of functional core promoter motifs (such as the TATA-box, initiator [Inr], and downstream core promoter element [DPE]) that confer specific properties to the core ...
Li Jing J Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, 29 Xinquan Road, Fuzhou, 350001, Fujian, - - 2014
The clinical outcome of chronic myeloid leukemia (CML) patients has been changed dramatically due to the development of imatinib (IM). However, the emergence of IM resistance, commonly associated with point mutations within the BCR-ABL kinase domain, remains a major clinical problem. Here, we investigated the effects of E35, a novel ...
Kang In-Hong IH Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea; Research Center for Biomineralization Disorders and Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of - - 2014
Chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) is a potent transcription factor that represses osteoblast differentiation and bone formation. Previously we observed that stimuli for osteoblast differentiation, such as bone morphogenetic protein 2 (BMP2), inhibits COUP-TFII expression. This study was undertaken to identify BMP2-regulated and COUP-TFII-targeting microRNAs (miRNAs), and ...
Reilman Ewoud E Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, Hanzeplein 1, P.O. box 30001, 9700 RB Groningen, the - - 2014
Expression of particular drug transporters in response to antibiotic pressure is a critical element in the development of bacterial multidrug resistance, and represents a serious concern for human health. To obtain a better understanding of underlying regulatory mechanisms, we have dissected the transcriptional activation of the ATP-binding cassette (ABC) transporter ...
Smith Jennifer A JA Department of Microbiology and Immunobiology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, United - - 2014
An important step in the malignant progression of HPV-associated lesions is the dysregulation of expression of the viral E6 and E7 oncogenes. This is often achieved through the loss of expression of E2, which represses the HPV LCR promoter and E6/E7 expression. Our previous studies confirmed a role for Brd4 ...
Zhang Ling - - 2014
Mammalian spermatogenesis is a well-organized process of cell development and differentiation. Meiosis expressed gene 1 (MEIG1) plays an essential role in the regulation of spermiogenesis. To explore potential mechanisms of MEIG1's action, a yeast two-hybrid screen was conducted, and several potential binding partners were identified; one of them was membrane ...
Bartels Hanni - - 2014
BackgroundAll retroviruses synthesize essential proteins via alternatively spliced mRNAs. Retrovirus genera, though, exploit different mechanisms to accomplish this. The best studied of these retroviral, post-transcriptional effectors are the trans-acting Rev protein of lentiviruses and the cis-acting constitutive transport element (CTE) of the betaretrovirus Mason-Pfizer monkey virus (MPMV). How members of ...
Zhang Lingzhi L 1 Department of Emergency, Shengjing Hospital of China Medical University , Shenyang, China - - 2014
Mood stabilizers lithium and valproates are widely used in the treatment of bipolar disorder. It has been shown that these drugs can affect the inositol monophosphatase activity and thus the inositol de novo biosynthesis. However, the molecular mechanism of this action has thus far been vague. As such, characterizing the ...
Snyder Marylynn M Cornell University Weill Medical College, United - - 2014
IL-6 mediated activation of Stat3 (Signal Transducer and Activator of Transcription 3) is a major signaling pathway in the process of breast cancer metastasis. One important mechanism by which the IL-6/STAT3 pathway promotes metastasis is through transcriptional regulation of the actin-bundling protein fascin. In this study, we further analyzed the ...
Lin Yanli Y Laboratory of Cell Engineering, Beijing Institute of Biotechnology, Beijing, P.R. - - 2014
NANOG plays important roles in neoplastic processes. However, the molecular mechanism of NANOG in tumorigenesis remains to be elucidated. In this report, we demonstrated that forced expression of NANOG in 293 cells and cancer cells led to increased c-Jun expression, whereas downregulation of endogenous NANOG significantly reduced c-Jun expression in ...
Zhang Jing - - 2014
Both KLF5 and Runx2 are involved in phenotypic switching of vascular smooth muscle cells (VSMC). However, the potential link between KLF5 and Runx2 in mediating vascular calcification remains unclear. The aim of the study was to elucidate the actual relationship between KLF5 and Runx2 in mediating VSMC calcification. We found ...
Hou Juan J Terry Fox Laboratory, BC Cancer Agency, Vancouver, British Columbia, V5Z 1L3, - - 2014
Although many regulatory networks involved in defining definitive endoderm have been identified, the mechanisms through which these networks interact to pattern the endoderm are less well understood. To explore the mechanisms involved in midgut patterning, we dissected the transcriptional regulatory elements of nephrocan (Nepn), the earliest known midgut specific gene ...
Pan Jun J The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510000, P.R. - - 2014
Immune responsive gene 1 (IRG1) is highly expressed in mammalian macrophages during inflammation. However, the role of IRG1 in tumorigenesis remains unclear. In the present study, we aimed to clarify the epigenetic regulation and biological functions of IRG1 in glioma. We found that the expression level of IRG1 influenced the ...
Kim Hye Young HY Department of Anatomy and Cell Biology, Dong-A University College of Medicine and Mitochondria Hub Regulation Center, Busan 602-714, South - - 2014
Previous studies have revealed that HBx expression has anti-apoptotic effects, resulting in increased drug resistance in HCC cells. Thus, we examined if sorafenib efficiently induces apoptosis in HBx-overexpressing HCC cells. Noticeably, sorafenib efficiently induced apoptosis, even in HBx-expressing HepG2 cells, indicating that the HBx protein does not attenuate sorafenib-induced apoptosis. ...
Grifone Raphaëlle R Sorbonne Universités, UPMC Univ Paris 06, UMR 7622, Laboratory of Developmental Biology, F-75005, Paris, France; CNRS, UMR 7622, Laboratory of Developmental Biology, F-75005, Paris, - - 2014
RNA-binding proteins (RBP) contribute to gene regulation through post-transcriptional events. Despite the important roles demonstrated for several RBP in regulating skeletal myogenesis in vitro, very few RBP coding genes have been characterized during skeletal myogenesis in vertebrate embryo. In the present study we report that Rbm24, which encodes the RNA-binding ...
Wei Fulan F School of Stomatology, Shandong University, Department of Orthodontics, Shandong Provincial Key Laboratory of Oral Biomedicine, China ; - - 2014
MicroRNAs (miRNAs) are short 20- to 22- nucleotide noncoding RNAs that negatively regulate the expression of target genes at the posttranscriptional level. The expression of specific miRNAs and their roles in the osteogenic differentiation of human periodontal ligament stem cells (PDLSCs) exposed to mechanical stretch remain unclear. Here we found ...
Lan H Y HY Department of Medical Microbiology and Parasitology, Fourth Military Medical University, Xi'an, 710032, - - 2014
It is well known that NS3/4A protein plays crucial roles in the hepatitis C virus (HCV) replication. NS3/4A protein also results to virus-mediated immune evasion and persistence of infection through the interaction with host proteins. However, the lack of a suitable animal model hampers studies of HCV NS3/4A protein interaction ...
Li Xinxin - - 2014
Follistatin (FST) is essential for skeletal muscle development, but the intracellular signaling networks that regulate Follistatin-induced effects are not well defined. We sought to investigate whether FST promotes the proliferation of myoblasts through the phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling. In this study, we transfected the pEGFP-duFST plasmid ...
Hsu Chih-Kai CK Department of Physiology and Pharmacology and Health Aging Research Center, College of Medicine, Chang Gung University, Kwei-San, Tao-Yuan, - - 2014
Sphingosine-1-phosphate (S1P) has been shown to regulate cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2 ) expression and IL-6 secretion in various respiratory diseases. However, the mechanisms underlying S1P-induced COX-2 expression and PGE2 production in human tracheal smooth muscle cells (HTSMCs) remain unclear. Here we demonstrated that S1P markedly induced COX-2 expression. S1P also ...
Sebbag M M UMR 5165 CNRS, 1056 INSERM, University of Toulouse, Toulouse, - - 2014
We read with great interest the recent article by Tan et al. (1) who looked for the expression of FLG, the gene encoding filaggrin, in the epithelial cells of mucosae of the oral cavity. FLG non-sense mutations are the strongest genetic risk factor for atopic dermatitis (2) and were shown to ...
Byrum Christine A CA Department of Biology, College of Charleston, Rita Liddy Hollings Science Center, 58 Coming Street, Room 214, Charleston, South Carolina, USA. Electronic address: - - 2014
Karyopherin alpha (KAP-α) proteins are critical for transport of many molecules into the nucleus. In this study, we identified three members of the KAP-α family in the sea urchin Lytechinus variegatus and described developmental expression of these proteins. Although many importins are assumed to have ubiquitous expression, we found that ...
Zhang Yuanyuan Y Department of Cardiology, Southwest Hospital, Third Military Medical University, Gaotanyan St, Shapingba District, Chongqing, 400038, People's Republic of - - 2014
Geminin has been correlated with vascular smooth muscle cell (VSMC) proliferation, but its mechanism is unclear. We selectively silenced the geminin gene of rat VSMCs by using RNAi technology and examined how geminin regulated VSMC proliferation. By using RNA interference in A10 cells and flow cytometry, (3)H-thymidine and 5-ethynyl-2'-deoxyuridine (EdU) ...
Jin Changzhong C State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, - - 2014
SAM domain and HD domain 1 (SAMHD1) is a newly discovered human immunodeficiency virus (HIV)-1 host restriction factor with high expression in HIV-1-non-permissive cells and low expression in HIV-1-permissive cells. The regulatory mechanism of SAMHD1 expression is still unclear. We examined the relationship between the expression levels of SAMHD1 mRNA ...
Gasparino Eliane E Department of Animal Science (sponsored by CNPq), State University of Maringá, av. Colombo, 5790, Maringá, PR, Brazil, - - 2014
In this study, we analysed markers of stress, plasma creatinine and T3 content, and insulin-like growth factor I (IGF-I), growth hormone receptor (GHR), uncoupling protein (UCP), adenine nucleotide translocase (ANT) and cytochrome c oxidase subunit III (COX III) mRNA expression in the liver and muscle of high (0.22 g/g) and low ...
1 2 3 4 5 6 7 8 9 10 >