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Kraus Petra P Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY, 13699, - - 2015
With the advent of BAC recombineering techniques, transcriptional promoter and enhancer isolation studies have become much more feasible in zebrafish than in mouse given the easy access to large numbers of fertilized zebrafish eggs and offspring in general, the easy to follow ex-utero development of zebrafish, an overall less skill ...
Le XiangDong X Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, - - 2015
MicroRNAs have the ability to alter and regulate multiple genes, including RTK family members, making them an attractive approach for molecular therapeutic development. We use a pCDNA6.2-EmGFP-microRNA expression vector to overexpress individual mature microRNA and then transfer the expression cassette into a single, inducible lentiviral vector (pINDUCER20). We successfully use ...
Peralta-Zaragoza Oscar O Direction of Chronic Infections and Cancer, Research Center in Infection Diseases, National Institute of Public Health, Av. Universidad No. 655, Colonia Santa María Ahuacatitlán, Cuernavaca, Morelos, 62100, Mexico, - - 2015
RNA interference is a natural mechanism to silence post-transcriptional gene expression in eukaryotic cells in which microRNAs act to cleave or halt the translation of target mRNAs at specific target sequences. Mature microRNAs, 19-25 nucleotides in length, mediate their effect at the mRNA level by inhibiting translation, or inducing cleavage ...
Abelius Martina S MS Division of Paediatrics, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden; Unit of Autoimmunity and Immune Regulation, Department of Clinical and Experimental Medicine, Linköping University, Linköping, - - 2014
How maternal allergy affects the systemic and local immunological environment during pregnancy and the immune development of the offspring is unclear. Expression of 40 genes was quantified by PCR arrays in placenta, peripheral blood mononuclear cells (PBMC), and cord blood mononuclear cells (CBMC) from 7 allergic and 12 non-allergic women ...
Goto Shingo S Disease Resistant Crops Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, - - 2014
The rice transcription factor WRKY45 plays a central role in the salicylic acid signalling pathway and mediates chemical-induced resistance to multiple pathogens, including Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae. Previously, we reported that rice transformants overexpressing WRKY45 driven by the maize ubiquitin promoter were strongly resistant to both pathogens; ...
Van Ba Hoa H Department of Animal Science and BK21 PLUS program, Chonbuk National University, Jeonju, 561-756, South - - 2014
Calpastatin participates in apoptotic cell death and cell signaling, but its role in skeletal myoblast development and molecular involvements in cell growth still remains unknown. The current study aimed to investigate the role of calpastatin on the expression patterns of calpains, caspases, and heat shock proteins (HSPs). In addition, the ...
Nagashima Takeshi T Laboratory for Integrated Cellular Systems, RIKEN Center for Integrative Medical Sciences (IMS), Tsurumi-ku, Yokohama, Kanagawa, 230-0045, - - 2014
ERK (extracellular signal-regulated kinase) plays a central role in signal transduction networks and cell fate decisions. Sustained ERK activation induces cell differentiation while transient ERK results in proliferation of several types of cells. Sustained ERK activity stabilizes proteins of early response gene products. However, the effect of ERK activity duration ...
Haftmann Claudia C Deutsches Rheuma-Forschungszentrum Berlin (DRFZ), An institute of the Leibniz Association Berlin, Berlin, - - 2014
Repeatedly activated T helper 1 (Th1) cells present during chronic inflammation can efficiently adapt to the inflammatory milieu, e.g. by expressing the transcription factor Twist1, which limits the immunopathology caused by Th1 cells. Here, we show that in repeatedly reactivated murine Th1 cells, Twist1 and T-bet induce expression of microRNA-148a ...
Pojednic Rachele M RM Nutrition, Exercise Physiology and Sarcopenia Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington St., Boston, MA, 02111, USA, - - 2014
Vitamin D receptor (VDR) expression and action in non-human skeletal muscle have recently been reported in several studies, yet data on the activity and expression of VDR in human muscle cells are scarce. We conducted a series of studies to examine the (1) effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on VDR ...
Wang Xiao-Feng XF Department of Neurology, the Third Affiliated Hospital of Southern Medical University, Guangzhou, - - 2014
Growth arrest and DNA-damage-inducible protein 45α (GADD45α) is an important member of the family of growth arrest and DNA damage-inducible (GADD) proteins. The expression patterns and possible roles of GADD45α in Parkinson's disease (PD) are so far less understood. In this study, we found that 1-methyl-4-phenylpyridinium (MPP+) treatment up-regulates the ...
Rowe Annette R AR Field of Microbiology, 220 Hollister Hall, Cornell University, Ithaca, NY, 14853, USA, - - 2014
To better understand the quantitative relationships between messenger RNA (mRNA) and protein biomarkers relevant to bioremediation, we quantified and compared respiration-associated gene products in an anaerobic syntrophic community. Respiration biomarkers for Dehalococcoides, an organohalide reducer, and Methanospirillum, a hydrogenotrophic methanogen, were quantified via qRT-PCR for mRNA and multiple reaction monitoring ...
Torii D D Department of Pharmacology, The Nippon Dental University School of Life Dentistry at Tokyo, Tokyo, - - 2014
Bone morphogenetic protein 7 (BMP-7) is a multifunctional differentiation factor that belongs to the transforming growth factor superfamily. BMP-7 induces gene expression of protein tyrosine phosphatase-like, member A/cementum attachment protein (PTPLA/CAP) and cementum protein 1 (CEMP1), both of which are markers of cementoblasts and cementocytes. In the previous study, we ...
Khamaisi Mogher M Institute of Endocrinology, Diabetes & Metabolism, Rambam Medical Center & RB Rappaport Faculty of Medicine-Technion, Haifa, - - 2014
Renal endothelin-converting enzyme (ECE)-1 is induced in experimental diabetes and following radiocontrast administration, conditions characterized by renal hypoxia, hypoxia-inducible factor (HIF) stabilization, and enhanced endothelin synthesis. Here we tested whether ECE-1 might be a HIF-target gene in vitro and in vivo. ECE-1 transcription and expression increased in cultured vascular endothelial ...
Chen Ting T Department of Cardiology, First Affiliated Hospital of Chinese People's Liberation Army General Hospital, Beijing, 100048, People's Republic of - - 2014
The adipose tissue-derived stem cells (ADSCs) represent a significant area of the cell therapy. Genetic modification of ADSCs may further improve their therapeutic potential. Here, we aimed to generate a lentiviral vector expressing insulin-like growth factor-I (IGF-1) and investigate the impact of IGF-1 transduction on the properties of cultured ADSCs. ...
Lee Seung-Eun SE Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, - - 2014
Tonoplast intrinsic proteins (TIPs) are integral membrane proteins that are known to function in plants as aquaporins. Here, we propose another role for TIPs during the fusion of protein storage vacuoles (PSVs) in aleurone cells, a process that is promoted by gibberellic acid (GA) and prevented by abscisic acid (ABA). ...
Lardenois Aurélie A Inserm U1085-Irset, Université de Rennes 1, Rennes, F-35042, - - 2014
It was recently reported that the sizes of many mRNAs change when budding yeast cells exit mitosis and enter the meiotic differentiation pathway. These differences were attributed to length variations of their untranslated regions. The function of UTRs in protein translation is well established. However, the mechanism controlling the expression ...
Cao Shan S University of Texas Medical School at Houston, Department of Neurosurgery, Houston, Texas, USA; Department of Clinical Pharmacology, Xiangya Hospital and Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha, Hunan, - - 2014
Hypoxia/ischemia produces a large amount of reactive oxygen species (ROS) which may attack macromolecules and cause brain injury. Nuclear factor (erythroid-derived 2)-like 2, (Nrf2), a strong transcriptional activator of antioxidant responsive element (ARE) responsive genes, can counteract the oxidative injury by regulating expression of several anti-oxidative genes in response to ...
Su Zhenzhong - - 2014
RNA binding motif 5 (RBM5) is a tumor suppressor gene that modulates apoptosis through the regulation of alternative splicing of apoptosis-related genes. Our previous studies suggested that RBM5 expression was negatively correlated with the expression of epidermal growth factor receptor (EGFR) in non-small cell lung cancer (NSCLC) tissues. This study ...
Brouwers Bas B Laboratory for Biochemical Neuroendocrinology, Department of Human Genetics, KU Leuven, Leuven 3000, - - 2014
The human growth hormone (hGH) minigene is frequently used in the derivation of transgenic mouse lines to enhance transgene expression. Although this minigene is present in the transgenes as a secondcistron, and thus not thought to be expressed, we found that three commonly used lines, Pdx1-Cre(Late), RIP-Cre, and MIP-GFP, each ...
Chhabra Ravindresh R Department of Biotechnology, Panjab University, Department of Biotechnology, Panjab University, Sector-14, Chandigarh 160014 (India). ravindresh@pu.ac.in, - - 2014
miRNAs and DNA methylation are both critical regulators of gene expression. Aberration in miRNA expression or DNA methylation is a causal factor for numerous pathological conditions. DNA methylation can inhibit the transcription of miRNAs, just like coding genes, by methylating the CpG islands in the promoter regions of miRNAs. Conversely, ...
Tsai Tsung-Ting TT Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan, Taiwan; Department of Orthopaedic Surgery, Spine Section, Chang Gung Memorial Hospital, Taoyuan, - - 2014
Sulfatase 1 (SULF1) plays a key role in cell signaling involving in cell growth, differentiation, proliferation, and migration. Abnormal SULF1 expression has been implicated in the development of various cancers and diseases of the skeletal and nervous systems. The present study aims to examine the difference in SULF1 expression between ...
Li Zhengrong Z Department of Gastrointestinal Surgery, First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi Province 330000, China. Electronic address: - - 2014
Phosphatase of regenerating liver-3 (PRL-3) is believed to be associated with cell motility, invasion, and metastasis. Our previous work found that PRL-3 is highly overexpressed in gastric cancer (GC) tissue with peritoneal metastasis and directly involved in the pathogenesis of GC peritoneal metastasis. Moreover, we further found that the down-regulation ...
Gupta Monica M Gene Regulation Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology (IIT) Bombay,Powai, Mumbai 400 076, India, - - 2014
Alpha-amylase (amyE) is one of the major exo-enzymes secreted by Bacillus subtilis during the post-exponential phase. The DegS-DegU two-component system regulates expression of majority of post-exponentially expressed genes in B. subtilis. It has been demonstrated that varying levels of the phosphorylated form of DegU (DegU-P) control different cellular processes. Exo-protease ...
Mohan Mahesh M Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA 70433 - - 2014
Persistent gastrointestinal inflammation, a hallmark of progressive HIV/SIV infection, causes disruption of the gastrointestinal epithelial barrier, microbial translocation, and generalized immune activation/inflammation driving AIDS progression. Apart from protein regulators, recent studies strongly suggest critical roles for microRNAs (miRNAs) in regulating and managing certain aspects of the inflammatory process. To examine ...
Fang Changxun C Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 35002, P. R. China; Key Laboratory of Crop Ecology and Molecular Physiology of Fujian Universities, Fujian Agriculture and Forestry University, Fuzhou 35002, P. R. - - 2014
Rice allelopathy is a hot topic in the field of allelopathy and behavior of donor allelopathic rice has been well documented. However, few study addresses response of receiver barnyardgrass (BYG). We found that expression of miRNAs relevant to plant hormone signal transduction, nucleotide excision repair and the peroxisome proliferator-activated receptor ...
Ding Xiao-Fei XF Taizhou University, - - 2014
NEK8 (never in mitosis gene A (NIMA)-related kinase 8) is involved in cytoskeleton, cilia and DNA damage response/repair. Abnormal expression and/or dysfunction of NEK8 are related to cancer development and progression. However, the mechanisms that regulate NEK8 are not well declared. We demonstrated here that pVHL may be involved in ...
Zhang Lingzhi L 1 Department of Emergency, Shengjing Hospital of China Medical University , Shenyang, China - - 2014
Mood stabilizers lithium and valproates are widely used in the treatment of bipolar disorder. It has been shown that these drugs can affect the inositol monophosphatase activity and thus the inositol de novo biosynthesis. However, the molecular mechanism of this action has thus far been vague. As such, characterizing the ...
Meng F F AgResearch, Ruakura Research Centre, Hamilton, New - - 2014
The inner cell mass (ICM) of mammalian blastocysts comprises 2 transient lineages, namely hypoblast and epiblast, which develop into extra-embryonic and embryonic tissues, respectively. In the mouse, epiblast cells autocrinally secrete fibroblast growth factor (FGF) to induce hypoblast differentiation, and pharmacological FGF/mitogen-activated protein kinase (MAPK) signal inhibition converts all ICM ...
Kaiser de Souza D D University of Brasilia, Brasilia, DF, - - 2014
The aim of the present study was to access the function of PI3-K pathway tested by the use of its inhibitor, LY294002, in maturation medium of bovine cumulus-oocyte complexes (COC) in steroid concentration in the medium, key enzymes of steroidogenic pathway and gonadotrophin receptors of cumulus cells. This study was ...
Ahn C C College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, South - - 2014
Tight junctions (TJ) are composed of a branching network of sealing strands. TJ regulate paracellular conductance and ionic selectivity. TJ components include the peripheral protein ZO-1, junctional adhesion molecules (JAM) and the integral proteins such as occludin and claudin. Claudins are a family of proteins that are the most important ...
Wohlres-Viana S S Embrapa, Juiz de Fora, MG, - - 2014
Embryo production in vivo is highly variable among donors. The Gir breed (Bos indicus) is well known to show a low embryo production after superovulation (2.5 to 3.5 viable embryos per flush), and a high variance in superovulatory responses, which makes this breed an interesting model to study this trait. ...
Go R-E RE Laboratory of Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of - - 2014
Fenhexamid, fludioxonil, and cyprodinil are antifungal agents used in agricultural applications, which are present at measurable amounts in fruits and vegetables. The induction of CYP gene expression including CYP1A1 and CYP1B1 is mediated by the transformation of polycyclic aromatic hydrocarbons (PAHs), and modulated by aryl hydrocarbon receptor (AhR). CYP1A1 is ...
Burda Whittney N - - 2014
BackgroundWe previously identified an ECF sigma factor, ¿S, that is important in the stress and virulence response of Staphylococcus aureus. Transcriptional profiling of sigS revealed that it is differentially expressed in many laboratory and clinical isolates, suggesting the existence of regulatory networks that modulates its expression.ResultsTo identify regulators of sigS, ...
Khongphinitbunjong Kitiphong K Bee Protection Center, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, - - 2014
The viral levels and immune responses of Italian honey bees (IHB), Russian honey bees (RHB) and an outcross of Varroa Sensitive Hygienic bees (POL) deliberately infested with one or two foundress Varroa were compared. We found that the Deformed wing virus (DWV) level in IHB inoculated with one or two ...
Tan Jin J Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, 110# Xiangya Road, Changsha, Hunan, 410008, People's Republic of - - 2014
Heat shock factor 1 (HSF1) is one of the most important transcriptional molecules in the heat shock process; however, HSF1 can also regulate the expression of other proteins. Dystrophin Dp71 is one of the most widely expressed isoforms of the dystrophin gene family. In our experiments, we showed for the ...
Chavoshinejad R - - 2014
The aim of the present study was to investigate the hormonal regulation of hyaluronan (HA) components in sheep granulosa cells. HA components are present in the reproductive tract and have a range of physical and signalling properties related to reproductive function in several species. First, abattoir-derived ovaries of sheep were ...
Tapia-Castillo Alejandra A Endocrinology, Escuela de Medicina, Pontificia Universidad Catolica de Chile, Santiago, - - 2014
Rac1 upregulation has been implicated in salt-sensitive hypertension as a modulator of mineralocorticoid receptor (MR) activity. Rac1 could affect the expression of oxidative stress markers, such as hemoxigenase-1 (HO-1) or nuclear factor-B (NF-κB), and the expression of neutrophil gelatinase-associated lipocalin (NGAL), a cytokine upregulated upon MR activation. We evaluated RAC1 ...
Eleftheriadis Stefanos - - 2014
Images of facial expressions are often captured from various views as a result of either head movements or variable camera position. Existing methods for multi-view and/or view-invariant facial expression recognition typically perform classification of the observed expression by using either classifiers learned separately for each view or a single classifier ...
Li An-Ying AY Department of Cardiology, The Third Xiangya Hospital of Central South University, No.138, Tongzipo Road, Yuelu District, Changsha, 410013, Hunan Province, - - 2014
Myocardial hypoxia is a major cause of cardiac dysfunction due to its triggering cell injury and apoptosis. Deregulated microRNAs and their roles in cardiomyocyte apoptosis have attracted much attention. miR-133a is among the most abundant of the miRNAs present in the normal heart, and significant changes in expression of miR-133a ...
Bebbere Daniela - - 2014
BackgroundThe sub-cortical maternal complex (SCMC), located in the subcortex of mouse oocytes and preimplantation embryos, is composed of at least four proteins encoded by maternal effect genes: OOEP, NLRP5/MATER, TLE6 and KHDC3/FILIA. The SCMC assembles during oocyte growth and was seen to be essential for murine zygote progression beyond the ...
Liu Ze-Qun ZQ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, PR - - 2014
Many studies have demonstrated that oxidative stress-induced apoptosis is a main cause of follicular atresia. Reactive oxygen species (ROS)-induced granulosa cell (GC) apoptosis is regulated by a variety of signaling pathways involving numerous genes and transcription factors. In this study, we found expression of the p53-upregulated modulator of apoptosis (PUMA), ...
KleszczyƄski Konrad K Department of Dermatology, University of Lübeck, Ratzeburger Allee 160, 23538, Lübeck, - - 2014
Melatonin, a lipophilic compound synthesized and released from the pineal gland, effectively acts against ultraviolet radiation (UVR), one of the main inducers of epidermal damage, skin cancer, inflammation, and DNA photodamage. One of the common known stress protein induced by UVR is heat shock protein 70 (Hsp70), highly expressed in ...
Rana Satiander - - 2014
BackgroundPharmacological investigations position withanolides as important bioactive molecules demanding their enhanced production. Therefore, one of the pivotal aims has been to gain knowledge about complete biosynthesis of withanolides in terms of enzymatic and regulatory genes of the pathway. However, the pathway remains elusive at the molecular level. P450s monooxygenases play ...
Song Rui R Department of Surgery, University of Colorado Denver, Box C-320, 12700 E 19th Avenue, Aurora, CO, 80045, - - 2014
Biglycan accumulates in aortic valves affected by calcific aortic valve disease (CAVD), and soluble biglycan upregulates BMP-2 expression in human aortic valve interstitial cells (AVICs) via Toll-like receptor (TLR) 2 and induces AVIC pro-osteogenic reprogramming, characterized by elevated pro-osteogenic activities. We sought to identify the factors responsible for biglycan-induced pro-osteogenic ...
Byrne Leah C - - 2014
Alternative splicing of nucleoredoxin-like 1 (Nxnl1) results in 2 isoforms of the rod-derived cone viability factor. The truncated form (RdCVF) is a thioredoxin-like protein secreted by rods that promotes cone survival, while the full-length isoform (RdCVFL), which contains a thioredoxin fold, is involved in oxidative signaling and protection against hyperoxia. ...
Chalei Vladislava V MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United - - 2014
Many intergenic long noncoding RNA (lncRNA) loci regulate the expression of adjacent protein coding genes. Less clear is whether intergenic lncRNAs commonly regulate transcription by modulating chromatin at genomically distant loci. Here, we report both genomically local and distal RNA-dependent roles of Dali, a conserved central nervous system expressed intergenic ...
Oji Yusuke Y Department of Cancer Stem Cell Biology, Osaka University Graduate School of Medicine, Osaka, - - 2014
The Wilms' tumor gene WT1 is overexpressed in leukemia and various types of solid tumors and plays an oncogenic role in these malignancies. Alternative splicing at two sites yields four major isoforms, 17AA(+)KTS(+), 17AA(+)KTS(-), 17AA(-)KTS(+), and 17AA(-)KTS(-), and all the isoforms are expressed in the malignancies. However, among the four ...
Wang Li L Pathology and Laboratory Medicine, McAllister Heart Institute, University of North - - 2014
Rationale: Generation of induced cardiac myocytes (iCMs) directly from fibroblasts offers great opportunities for cardiac disease modeling and cardiac regeneration. A major challenge of iCM generation is the low conversion rate of fibroblasts to fully reprogrammed iCMs, which could in part be attributed to unbalanced expression of reprogramming factors Gata4 ...
Lu Zhipeng Z University of North - - 2014
Pre-mRNA splicing is a critical step in eukaryotic gene expression that contributes to proteomic, cellular and developmental complexity. Small nuclear (sn)RNAs are core spliceosomal components, however, the extent to which differential expression of snRNA isoforms regulates splicing is completely unknown. This is partly due to difficulties in the accurate analysis ...
Cai Rong R Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai, - - 2014
Defect in mitochondrial biogenesis and cardiac energy metabolism is a critical contributing factor to cardiac hypertrophy and heart failure. Sentrin/SUMO specific protease 1 (SENP1) mediated regulation of PGC-1α transcriptional activity plays an essential role in mitochondrial biogenesis and mitochondrial function. However, whether SENP1 plays a role in cardiac hypertrophy and ...
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