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Kraus Petra P Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY, 13699, - - 2015
With the advent of BAC recombineering techniques, transcriptional promoter and enhancer isolation studies have become much more feasible in zebrafish than in mouse given the easy access to large numbers of fertilized zebrafish eggs and offspring in general, the easy to follow ex-utero development of zebrafish, an overall less skill ...
Le XiangDong X Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, - - 2015
MicroRNAs have the ability to alter and regulate multiple genes, including RTK family members, making them an attractive approach for molecular therapeutic development. We use a pCDNA6.2-EmGFP-microRNA expression vector to overexpress individual mature microRNA and then transfer the expression cassette into a single, inducible lentiviral vector (pINDUCER20). We successfully use ...
Sakamoto Hiroshi H Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto City, 860-0811, - - 2014
The transcription factor c-Myb was originally identified as a transforming oncoprotein encoded by two avian leukaemia viruses. Subsequently, through the generation of mouse models that affect its expression, c-Myb has been shown to be a key regulator of hematopoiesis, including having critical roles in hematopoietic stem cells (HSC). The precise ...
Duan Jin J Department of Biology, University of Waterloo, Waterloo, ON, Canada, N2L - - 2014
Bacteria often have multiple copies of ribosomal RNA (rrn) genes in their genomes. The presence of multiple rrn operons suggests an advantage to the organism, perhaps through adjustable control of protein expression in response to altered environmental conditions. In the work described here, the strengths of the seven rRNA promoters ...
Hofmann Alejandro D AD National Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin 12, - - 2014
The high morbidity and mortality in congenital diaphragmatic hernia (CDH) is attributed to pulmonary hypoplasia and persistent pulmonary hypertension (PH). PH is characterized by increased pulmonary artery smooth muscle cell (SMC) proliferation, suppressed apoptosis as well as endothelial dysfunction. Krüppel-like factor 5 (KLF5) belongs to a family of transcription factors ...
Tsotakos Nikolaos N Penn State University College of - - 2014
Surfactant Protein A (SP-A), a molecule with roles in lung innate immunity and surfactant-related functions, is encoded by two genes in humans: SFTPA1 (SP-A1) and SFTPA2 (SP-A2). The mRNAs from these genes differ in their 5' untranslated regions (5'UTR) due to differential splicing. The 5'UTR variant ACD' is exclusively found ...
Luebke Justin L JL Department of Chemistry, Indiana University, Bloomington, IN 47405-7102 - - 2014
How cells regulate the bioavailability of utilizable sulfur while mitigating the effects of hydrogen sulfide toxicity is poorly understood. CstR (Copper-sensing operon repressor (CsoR)-like sulfurtransferase repressor) represses the expression of the cst operon encoding a putative sulfide oxidation system in Staphylococcus aureus. Here, we show that the cst operon is ...
McCulloch Katherine A KA Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN - - 2014
MicroRNAs (miRNAs) are small RNAs that regulate gene expression posttranscriptionally via the 3' UTR of target mRNAs and were first identified in the Caenorhabditis elegans heterochronic pathway. miRNAs have since been found in many organisms and have broad functions, including control of differentiation and pluripotency in humans. lin-4 and let-7-family ...
Kim MinJung M Departments of Physiology and Pediatrics, Center for Stem Cell Biology & Regenerative Medicine, Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD, - - 2014
Expression levels of MIR144 and MIR451 increase during erythropoiesis, a pattern that is conserved from zebrafish to humans. As these two miRs are expressed from the same polycistronic transcript, we manipulated MIR144 and MIR451 in human erythroid cells individually and together to investigate their effects on human erythropoiesis. Inhibition of ...
Chandra Amaresh A Division of Plant Physiology and Biochemistry, Indian Institute of Sugarcane Research, Lucknow, - - 2014
Sucrose synthesis/accumulation in sugarcane is a complex process involving many genes and regulatory sequences that control biochemical events in source-sink tissues. Among these, sucrose synthase (SuSy), sucrose phosphate synthase (SPS), soluble acid (SAI) and cell wall (CWI) invertases are important. Expression of these enzymes was compared in an early (CoJ64) ...
Chen Ana A National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, - - 2014
Bacillus acidopullulyticus pullulanase (BaPul13A) is a widely used debranching enzyme in the starch industry. A few details have been reported on the heterologous expression of BaPul13A in Escherichia coli (E. coli). This study compares different E. coli expression systems to improve the soluble expression level of BaPul13A. When pET22b(+)/pET28a(+) was ...
Pabona John Mark P JM Department of Physiology & Biophysics, University of Arkansas for Medical Sciences, 4301 W Markham St., Little Rock, AR, - - 2014
Context: Late term pregnancy may lead to maternal and neonatal morbidity and mortality. Mice null for the progesterone receptor co-regulator Krüppel-like Factor 9 (KLF9) exhibit delayed parturition and increased incidence of neonatal deaths. Objective: Our aim is to evaluate the contribution of myometrial KLF9 to human parturition. Design: Myometrial biopsies ...
Lucas C C Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland; Institut de Recherche sur la Biologie de l'Insecte (UMR 7261), CNRS, University of Tours, Tours, - - 2014
The aim of this study was to investigate levels of expression of two major genes, the odorant binding protein Gp-9 (general protein-9) and foraging, that have been shown to be associated with behavioural polymorphisms in ants. We analysed workers and young nonreproductive queens collected from nests of the monogyne (single ...
Otabe Koji K Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, MEM-161, La Jolla, 92037, California; Department of System Biomedicine, National Research Institute for Child Health and Development, Tokyo, Japan; Center for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, Tokyo, - - 2014
Mohawk homeobox (MKX) has been demonstrated as a tendon/ligament specific transcription factor. The aim of this study was to investigate the role of MKX in ligament/tenogenic differentiation of bone marrow derived mesenchymal stem cells (BMMSCs). Human BMMSCs were treated with 50 ng/ml BMP-12 or transduced with MKX or scleraxis (SCX) adenoviral ...
Wu Zhuomin Z Center for Drug Research and Development, Zhujiang Hospital, Southern Medical University, Zhujiang, - - 2014
Long non-coding RNAs (lncRNAs) are series of transcripts with important biological functions. Various diseases have been associated with aberrant expression of lncRNAs and the related dysregulation of mRNAs. In this review, we highlight the mechanisms of dynamic lncRNA expression. The chromatin state contributes to the low and specific expression of ...
Pesch Martina M Biocenter, Cologne University, Botanical Institute, 50674 Cologne, - - 2014
Trichome patterning in Arabidopsis thaliana is regulated by a regulatory feedback loop of the trichome promoting factors TRANSPARENT TESTA GLABRA1 (TTG1), GLABRA3 (GL3)/ENHANCER OF GL3 (EGL3), and GL1 and a group of homologous R3MYB proteins that act as their inhibitors. Together, they regulate the temporal and spatial expression of GL2 ...
Kim Sung Jin SJ Department of Molecular Biology, Institute of Nanosensor and Biotechnology, Dankook University, Yongin 448-701, Republic of - - 2014
Low oxygen conditions, termed hypoxia, can affect cell survivals. Cells may adapt to hypoxic conditions through hypoxia response elements (HRE) such as erythropoietin enhancer or phosphoglycerate kinase element. Hypoxic conditions usually appear in solid tumors, and can cause resistance to radiotherapy or chemotherapy. In this study, a genetic approach based ...
Mishra Manish M Wayne State University, Detroit, 48201, United - - 2014
Purpose: Diabetes induces oxidative imbalance in the retina and impairs Nrf2-medaited antioxidant response, and Keap1, the cytoplasmic repressor of Nrf2, is elevated. The goal of this study is to understand the role of epigenetic modifications at Keap1 promoter in regulation of Nrf2 function. Methods: The effect of high glucose on ...
Zhang Lili L State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China College of Life Science, Northeast Forestry University, Harbin 150040, - - 2014
The regulation of light-dependent anthocyanin biosynthesis in Brassica rapa subsp. rapa cv. Tsuda turnip was investigated using an EMS-induced mutant R30 with light-independent pigmentation. TILLING and subsequent analysis showed that a stop codon was inserted in the R2R3-MYB transcription factor BrMYB4 and that the encoded protein (BrMYB4mu) had lost its ...
Kamiunten Taichi T Department of Pharmacology, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, 230-8501, - - 2014
Tissue-specific gene expression is subjected to epigenetic and genetic regulation. Posttranslational modifications of histone tails alter the accessibility of nuclear proteins to DNA, thus affecting the activity of the regulatory complex of nuclear proteins. Methylation at histone 3 lysine 9 (H3K9) is a crucial modification that affects gene expression and ...
Lydia Pernet P University Grenoble-Alpes, CRI INSERM, U823, Institut Albert Bonniot, Site Santé, BP 170 - La Tronche - 38042 GRENOBLE Cedex 9 - - - 2014
After heat-shock, HSF1 controls a major cellular transcriptional response involving the activation of early (Hsp70) and late (Hsp25) heat shock gene expression. Here we show that a full response to heat shock (activation of both Hsp70 and Hsp25) depends on the duration of HSF1 activation, which is itself controlled by ...
Zhang Wei-Hua - - 2014
BackgroundPulmonary arterial hypertension (PAH) is a proliferative arteriopathy associated with a glycolytic shift during heart metabolism. An increase in glycolytic metabolism can be detected in the right ventricle during PAH. Expression levels of glycolysis genes in the right ventricle during glycolysis that occur in monocrotaline (MCT)-induced pulmonary hypertension (PH) remain ...
Chun Rene F RF Department of Orthopaedic Surgery, UCLA-Orthopaedic Hospital, Los Angeles, CA, 90095, United - - 2014
Activation of precursor 25-hydroxyvitamin D3 (25D) to hormonal 1,25-dihydroxyvitamin D3 (1,25D) is a pivotal step in vitamin D physiology, catalysed by the enzyme 25-hydroxyvitamin D-1α-hydroxylase (1α-hydroxylase). To establish new models for assessing the physiological importance of the 1α-hydroxylase-25D-axis, we used Danio rerio (zebrafish) to characterize expression and biological activity of ...
Gao Feng F Department of Cardiology, Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou, 310009, People's Republic of - - 2014
The objective of this study was to determine the role of Hsp90α in regulating the migration of mesenchymal stem cells (MSCs) and to investigate the underlying mechanisms of this effect. MSCs migration was assessed by wound healing assay and transwell migration assay. Hsp90α expression was silenced in MSC by siRNA ...
Kondo Hidehiro H Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Minato, Tokyo, Japan. Electronic address: - - 2014
Pathogenic bacteria possess some components, that are recognized by the host immune receptors. Different components are recognized by distinct receptors, and thus it is speculated that these components may regulate different gene sets. To investigate the gene expression profiles regulated by different bacterial components in Japanese flounder Paralichthys olivaceus, Japanese ...
Chi Jing-Ruei JR Department of Biochemical Science and Technology, National Taiwan University, Taipei, - - 2014
The hepcidin gene is widely expressed in many fish species and functions as an antimicrobial peptide, suggesting that it plays an important role in the innate immune system of fish. In the present study, the Amatitlania nigrofasciata hepcidin gene (AN-hepc) was cloned from the liver and its expression during an ...
Takeda Shuso S Department of Molecular Biology, Drug Innovation Research Center, Daiichi University of Pharmacy, 22-1 Tamagawa-cho, Minami-ku, Fukuoka 815-8511, Japan; Laboratory of Xenobiotic Metabolism and Environmental Toxicology, Faculty of Pharmaceutical Sciences, Hiroshima International University (HIU), 5-1-1Hiro-koshingai, Kure, Hiroshima 737-0112, - - 2014
We recently reported that 9-tetrahydrocannabinol (9-THC), a major cannabinoid component in Cannabis Sativa (marijuana), significantly stimulated the expression of fatty acid 2-hydroxylase (FA2H) in human breast cancer MDA-MB-231 cells. Peroxisome proliferator-activated receptor  (PPAR) was previously implicated in this induction. However, the mechanisms mediating this induction have not been elucidated ...
Wan Zhong-Yuan - - 2014
IntroductionIn addition to the well-known short noncoding RNAs such as microRNAs (miRNAs), increasing evidence suggests that long noncoding RNAs (lncRNAs) act as key regulators in a wide aspect of biologic processes. Dysregulated expression of lncRNAs has been demonstrated being implicated in a variety of human diseases. However, little is known ...
Kakuda Tsutomu - - 2014
Background Rhodococcus equi is an important pulmonary pathogen in foals and in immunocompromised individuals. Virulent R. equi strains carry an 80-90 kb virulence plasmid that expresses the virulence-associated protein A (VapA). VapA expression is regulated by temperature and pH. The LysR-type transcriptional regulator, VirR, is involved in the regulation of the ...
Chen Kuan-Jung KJ Academia - - 2014
The Single-minded 2 (SIM2) protein is a bHLH transcription factor regulating CNS development in Drosophila. In humans, SIM2 is located within the Down syndrome critical region on chromosome 21 and may be involved in the development of mental retardation phenotype in Down syndrome. In this study, knockout of SIM2 expression ...
Gruber Steffen S Institute of Molecular Biotechnology, Graz University of Technology, NAWI Graz, Petersgasse 14/1, A-8010 Graz, - - 2014
The Gram-negative β-proteobacterium Ralstonia eutropha H16 is primarily known for polyhydroxybutyrate (PHB) production and its ability to grow chemolithoautotrophically by using CO2 and H2 as sole carbon and energy sources. The majority of metabolic engineering and heterologous expression studies conducted so far rely on a small number of suitable expression ...
Han Lin L Department of Nephrology, Yangpu Hospital,TongJi - - 2014
Abstract Context: Lead is a common industrial toxicant and has been proved to be associated with the kidney damage. Objective : To investigate the effect and mechanism of lead on expression of rat renal interstitial fibroblast activation related protein. Materials and Methods: The expression of activation related protein mRNA was ...
Ding Qi - - 2014
BackgroundTrees in temperate zones show periodicity by alternating active and dormant states to adapt to environmental conditions. Although phytohormones and transcriptional regulation were found to be involved in growth cessation and dormancy transition, little is known about the mechanisms of the dormancy-active growth transition, especially dormancy maintenance and release. Small ...
Tang Mingfeng - - 2014
Independent transcriptome profile analyses of miRNAs or mRNAs under conditions of cadmium (Cd) stress have been widely reported in plants. However, a combined analysis of sRNA sequencing expression data with miRNA target expression data to infer the relative activities of miRNAs that regulate gene expression changes resulting from Cd stress ...
Zou Yan-Mei YM Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China, - - 2014
Reduced radiosensitivity of lung cancer cells represents a pivotal obstacle in clinical oncology. The hypoxia-inducible factor (HIF)-1α plays a crucial role in radiosensitivity, but the detailed mechanisms remain elusive. A relationship has been suggested to exist between hypoxia and autophagy recently. In the current study, we studied the effect of ...
Ren Cong - - 2014
DNA polymerase eta (POLH), a target of p53 tumor suppressor, plays a key role in translesion DNA synthesis (TLS). Loss of POLH is responsible for human cancer prone syndrome, Xeroderma Pigmentosum Variant (XPV). Due to its critical role in DNA repair and genome stability, POLH expression and activity are regulated ...
Haltaufderhyde Kirk K Department of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Providence, Rhode Island, 02912, - - 2014
Human skin is constantly exposed to solar light containing visible and ultraviolet radiation (UVR), a powerful skin carcinogen. UVR elicits cellular responses in epidermal cells via several mechanisms: direct absorption of short wavelength UVR photons by DNA, oxidative damage caused by long wavelength UVR, and, as we recently demonstrated, via ...
Cheng Yuqiang - - 2014
TLR5 is responsible for the recognition of bacterial flagellin in vertebrates. In the present study, the first TLR5 gene in duck was cloned. The ORF of duck TLR5 (dTLR5) cDNA is 2 580 bp and encodes a polypeptide of 859 amino acids. We also cloned partial sequences of myeloid differentiation ...
Johnson Justin S JS Indiana University School of Medicine, United - - 2014
While the Pancreatic duodenal homeobox 1 (Pdx-1) transcription factor is known to play an indispensable role in β cell development and secretory function, recent data also implicate Pdx-1 in the maintenance of endoplasmic reticulum (ER) health. The sarco endoplasmic reticulum Ca(2+) ATPase 2b (SERCA2b) pump maintains a steep Ca(2+) gradient ...
Lukowski Samuel W SW School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia Australian Equine Genetics Research Centre, The University of Queensland, Queensland, 4072, Australia - - 2014
Post-transcriptional regulation of gene expression through 5'UTR-encoded cis-acting elements is an important mechanism for the control of protein expression levels. Through controlling specific aspects of translation initiation, expression can be tightly regulated whilst remaining responsive to cellular requirements. With respect to Cystic Fibrosis, the overexpression of CFTR protein trafficking mutants, ...
Yamagami Kazuki K Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka 812-8581, - - 2014
The forkhead box a (Foxa) protein family has been found to play important roles in mammals. Recently, the expression of Foxa2 was reported in the mouse uterus, and it was reported to be involved in regulation of implantation. However, the regulation of Foxa2 expression in the uterus is still poorly ...
Brocato Jason J NYU School of Medicine, United - - 2014
The replication-dependent histone genes are the only metazoan genes whose messenger RNA (mRNA) does not terminate with a poly(A) tail at the 3' end. Instead, the histone mRNAs display a stem-loop structure at their 3' end. Stem-loop binding protein (SLBP) binds the stem-loop and regulates canonical histone mRNA metabolism. Here ...
Itoh Kazuko K Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, Faculty of Life Sciences, 1.1.1. Honjo, Chuo-ku, Kumamoto, Kumamoto 860-8556, Japan; Department of Internal Medicine and Education & Research Center, Kitasato University Medical Center, 6-100 Arai, Kitamoto, Saitama 364-8501, - - 2014
Sodium reabsorption via Na-K-2Cl cotransporter 2 (NKCC2) in the thick ascending limbs has a major role for medullary osmotic gradient and subsequent water reabsorption in the collecting ducts. We investigated intrarenal localization of three isoforms of NKCC2 mRNA expressions and the effects of dehydration on them in rats. To further ...
Zhan Rongrong R State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, - - 2014
Inulin fructotransferase (IFTase) catalyses inulin conversion to difructose anhydride (DFA III), which is a natural low-calorie sweetener. Although heterologous expression of IFTase was achieved in Escherichia coli, the extracellular enzyme activity was very low which limited the commercialization of IFTase. Active IFTase of about 43 kDa molecular mass of subunit ...
Bandopadhyay Manikankana - - 2014
Hepatitis B Virus (HBV) X protein (HBx) is known to be involved in the initiation and progression of hepatocellular carcinoma (HCC) through modulation of host gene response. Alterations in miRNA expressions are frequently noted in HCC. This study is aimed to examine the role of HBx protein in the modulation ...
Sun Piyun P Department of Neurology, The Fourth Affiliated Hospital, Harbin Medical University, No. 37 Jiyuan street, Harbin, Heilongjiang, 150001, - - 2014
Matrix metalloproteinase 3 (MMP-3) is implicated in the pathogenesis and progression of glioma. However, whether MMP-3 participates in the regulation of metastasis and its mechanisms in glioma is mostly unknown. In the present study, glioma cells were stably transfected with Bmi-1 small interfering RNA (siRNA) to knockdown off Bmi-1 or ...
Gajewska Alina A A Gajewska, Department of Neuroendocrinology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Jablonna n. Warsaw, 05-110, Poland - - 2014
EGR1 and PITX1 are transcription factors required for gonadotroph cell Lhb promoter activation. To determine changes in Egr1 and Pitx1 mRNA levels in central and peripheral pituitary stimulations, an in vivo model based on intracerebroventricular (i.c.v.) pulsatile (1 pulse/0.5 h over 2 h) GnRH agonist (1.5 nM buserelin) or antagonist ...
van der Lelij Petra P IMP Research Institute of Molecular Pathology, Vienna, - - 2014
Although splicing is essential for the expression of most eukaryotic genes, inactivation of splicing factors causes specific defects in mitosis. The molecular cause of this defect is unknown. Here, we show that the spliceosome subunits SNW1 and PRPF8 are essential for sister chromatid cohesion in human cells. A transcriptome-wide analysis ...
Jeong Sang Hoon SH Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology and Division of Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, - - 2014
The early growth response (Egr)-1 is a transcriptional factor which plays an important role in the regulation of cell growth, differentiation, cell survival and immune responses. Emerging evidences including our data demonstrate that the Egr-1 expression is up-regulated in the psoriatic skin lesions. The purpose of this study was to ...
Andrie Jennifer J University of - - 2014
Gene expression levels are determined by the balance between rates of mRNA transcription and decay, and genetic variation in either of these processes can result in heritable differences in transcript abundance. Although the genetics of gene expression has been the subject of intense interest, the contribution of heritable variation in ...
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