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Zhao Guangfeng - - 2014
Primary ovarian insufficiency (POI) is a serious reproductive dysfunction with the follicle pool reduced and depleted. Abnormal apoptosis of ovarian granulosa cells (GCs) is believed to result in follicle loss. Progesterone receptor membrane component (PGRMC) 1, critical for the GC survival, was reported to reduce in POI patients, but the ...
Cruz Cynthia Dela CD Division of Human Reproduction, Department of Obstetrics and Gynecology, Federal University of Minas Gerais, Belo Horizonte, - - 2014
Nodal is a growth factor of the transforming growth factor β superfamily that is expressed in high turnover tissues, such as the human endometrium, and in several malignancies. The effects of Nodal are modulated by the coreceptor Cripto and mediated by SMAD proteins. This study evaluated the gene and protein ...
Wang Chang-Quan CQ Department of Plant Biology, University of California, Davis, California - - 2014
The timely transition of vegetative to reproductive development is coordinated through quantitative regulation of floral pathway genes in response to physiological and environmental cues. Here, we show that the circadian-controlled expression of the Arabidopsis thaliana floral transition regulators FLOWERING LOCUS T (FT) and CONSTANS (CO) is antiphasic to that of ...
Kedmi Adi A The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, - - 2014
Transcription of protein-coding genes is highly dependent on the RNA polymerase II core promoter. Core promoters, generally defined as the regions that direct transcription initiation, consist of functional core promoter motifs (such as the TATA-box, initiator [Inr], and downstream core promoter element [DPE]) that confer specific properties to the core ...
Li Jing J Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, 29 Xinquan Road, Fuzhou, 350001, Fujian, - - 2014
The clinical outcome of chronic myeloid leukemia (CML) patients has been changed dramatically due to the development of imatinib (IM). However, the emergence of IM resistance, commonly associated with point mutations within the BCR-ABL kinase domain, remains a major clinical problem. Here, we investigated the effects of E35, a novel ...
Kang In-Hong IH Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea; Research Center for Biomineralization Disorders and Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of - - 2014
Chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) is a potent transcription factor that represses osteoblast differentiation and bone formation. Previously we observed that stimuli for osteoblast differentiation, such as bone morphogenetic protein 2 (BMP2), inhibits COUP-TFII expression. This study was undertaken to identify BMP2-regulated and COUP-TFII-targeting microRNAs (miRNAs), and ...
Bartels Hanni - - 2014
BackgroundAll retroviruses synthesize essential proteins via alternatively spliced mRNAs. Retrovirus genera, though, exploit different mechanisms to accomplish this. The best studied of these retroviral, post-transcriptional effectors are the trans-acting Rev protein of lentiviruses and the cis-acting constitutive transport element (CTE) of the betaretrovirus Mason-Pfizer monkey virus (MPMV). How members of ...
Reilman Ewoud E Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, Hanzeplein 1, P.O. box 30001, 9700 RB Groningen, the - - 2014
Expression of particular drug transporters in response to antibiotic pressure is a critical element in the development of bacterial multidrug resistance, and represents a serious concern for human health. To obtain a better understanding of underlying regulatory mechanisms, we have dissected the transcriptional activation of the ATP-binding cassette (ABC) transporter ...
Smith Jennifer A JA Department of Microbiology and Immunobiology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, United - - 2014
An important step in the malignant progression of HPV-associated lesions is the dysregulation of expression of the viral E6 and E7 oncogenes. This is often achieved through the loss of expression of E2, which represses the HPV LCR promoter and E6/E7 expression. Our previous studies confirmed a role for Brd4 ...
Zhang Lingzhi L 1 Department of Emergency, Shengjing Hospital of China Medical University , Shenyang, China - - 2014
Mood stabilizers lithium and valproates are widely used in the treatment of bipolar disorder. It has been shown that these drugs can affect the inositol monophosphatase activity and thus the inositol de novo biosynthesis. However, the molecular mechanism of this action has thus far been vague. As such, characterizing the ...
Snyder Marylynn M Cornell University Weill Medical College, United - - 2014
IL-6 mediated activation of Stat3 (Signal Transducer and Activator of Transcription 3) is a major signaling pathway in the process of breast cancer metastasis. One important mechanism by which the IL-6/STAT3 pathway promotes metastasis is through transcriptional regulation of the actin-bundling protein fascin. In this study, we further analyzed the ...
Lin Yanli Y Laboratory of Cell Engineering, Beijing Institute of Biotechnology, Beijing, P.R. - - 2014
NANOG plays important roles in neoplastic processes. However, the molecular mechanism of NANOG in tumorigenesis remains to be elucidated. In this report, we demonstrated that forced expression of NANOG in 293 cells and cancer cells led to increased c-Jun expression, whereas downregulation of endogenous NANOG significantly reduced c-Jun expression in ...
Zhang Jing - - 2014
Both KLF5 and Runx2 are involved in phenotypic switching of vascular smooth muscle cells (VSMC). However, the potential link between KLF5 and Runx2 in mediating vascular calcification remains unclear. The aim of the study was to elucidate the actual relationship between KLF5 and Runx2 in mediating VSMC calcification. We found ...
Hou Juan J Terry Fox Laboratory, BC Cancer Agency, Vancouver, British Columbia, V5Z 1L3, - - 2014
Although many regulatory networks involved in defining definitive endoderm have been identified, the mechanisms through which these networks interact to pattern the endoderm are less well understood. To explore the mechanisms involved in midgut patterning, we dissected the transcriptional regulatory elements of nephrocan (Nepn), the earliest known midgut specific gene ...
Pan Jun J The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510000, P.R. - - 2014
Immune responsive gene 1 (IRG1) is highly expressed in mammalian macrophages during inflammation. However, the role of IRG1 in tumorigenesis remains unclear. In the present study, we aimed to clarify the epigenetic regulation and biological functions of IRG1 in glioma. We found that the expression level of IRG1 influenced the ...
Kim Hye Young HY Department of Anatomy and Cell Biology, Dong-A University College of Medicine and Mitochondria Hub Regulation Center, Busan 602-714, South - - 2014
Previous studies have revealed that HBx expression has anti-apoptotic effects, resulting in increased drug resistance in HCC cells. Thus, we examined if sorafenib efficiently induces apoptosis in HBx-overexpressing HCC cells. Noticeably, sorafenib efficiently induced apoptosis, even in HBx-expressing HepG2 cells, indicating that the HBx protein does not attenuate sorafenib-induced apoptosis. ...
Grifone Raphaëlle R Sorbonne Universités, UPMC Univ Paris 06, UMR 7622, Laboratory of Developmental Biology, F-75005, Paris, France; CNRS, UMR 7622, Laboratory of Developmental Biology, F-75005, Paris, - - 2014
RNA-binding proteins (RBP) contribute to gene regulation through post-transcriptional events. Despite the important roles demonstrated for several RBP in regulating skeletal myogenesis in vitro, very few RBP coding genes have been characterized during skeletal myogenesis in vertebrate embryo. In the present study we report that Rbm24, which encodes the RNA-binding ...
Wei Fulan F School of Stomatology, Shandong University, Department of Orthodontics, Shandong Provincial Key Laboratory of Oral Biomedicine, China ; - - 2014
MicroRNAs (miRNAs) are short 20- to 22- nucleotide noncoding RNAs that negatively regulate the expression of target genes at the posttranscriptional level. The expression of specific miRNAs and their roles in the osteogenic differentiation of human periodontal ligament stem cells (PDLSCs) exposed to mechanical stretch remain unclear. Here we found ...
Lan H Y HY Department of Medical Microbiology and Parasitology, Fourth Military Medical University, Xi'an, 710032, - - 2014
It is well known that NS3/4A protein plays crucial roles in the hepatitis C virus (HCV) replication. NS3/4A protein also results to virus-mediated immune evasion and persistence of infection through the interaction with host proteins. However, the lack of a suitable animal model hampers studies of HCV NS3/4A protein interaction ...
Li Xinxin - - 2014
Follistatin (FST) is essential for skeletal muscle development, but the intracellular signaling networks that regulate Follistatin-induced effects are not well defined. We sought to investigate whether FST promotes the proliferation of myoblasts through the phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling. In this study, we transfected the pEGFP-duFST plasmid ...
Hsu Chih-Kai CK Department of Physiology and Pharmacology and Health Aging Research Center, College of Medicine, Chang Gung University, Kwei-San, Tao-Yuan, - - 2014
Sphingosine-1-phosphate (S1P) has been shown to regulate cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2 ) expression and IL-6 secretion in various respiratory diseases. However, the mechanisms underlying S1P-induced COX-2 expression and PGE2 production in human tracheal smooth muscle cells (HTSMCs) remain unclear. Here we demonstrated that S1P markedly induced COX-2 expression. S1P also ...
Sebbag M M UMR 5165 CNRS, 1056 INSERM, University of Toulouse, Toulouse, - - 2014
We read with great interest the recent article by Tan et al. (1) who looked for the expression of FLG, the gene encoding filaggrin, in the epithelial cells of mucosae of the oral cavity. FLG non-sense mutations are the strongest genetic risk factor for atopic dermatitis (2) and were shown to ...
Byrum Christine A CA Department of Biology, College of Charleston, Rita Liddy Hollings Science Center, 58 Coming Street, Room 214, Charleston, South Carolina, USA. Electronic address: - - 2014
Karyopherin alpha (KAP-α) proteins are critical for transport of many molecules into the nucleus. In this study, we identified three members of the KAP-α family in the sea urchin Lytechinus variegatus and described developmental expression of these proteins. Although many importins are assumed to have ubiquitous expression, we found that ...
Zhang Yuanyuan Y Department of Cardiology, Southwest Hospital, Third Military Medical University, Gaotanyan St, Shapingba District, Chongqing, 400038, People's Republic of - - 2014
Geminin has been correlated with vascular smooth muscle cell (VSMC) proliferation, but its mechanism is unclear. We selectively silenced the geminin gene of rat VSMCs by using RNAi technology and examined how geminin regulated VSMC proliferation. By using RNA interference in A10 cells and flow cytometry, (3)H-thymidine and 5-ethynyl-2'-deoxyuridine (EdU) ...
Jin Changzhong C State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, - - 2014
SAM domain and HD domain 1 (SAMHD1) is a newly discovered human immunodeficiency virus (HIV)-1 host restriction factor with high expression in HIV-1-non-permissive cells and low expression in HIV-1-permissive cells. The regulatory mechanism of SAMHD1 expression is still unclear. We examined the relationship between the expression levels of SAMHD1 mRNA ...
Gasparino Eliane E Department of Animal Science (sponsored by CNPq), State University of Maringá, av. Colombo, 5790, Maringá, PR, Brazil, - - 2014
In this study, we analysed markers of stress, plasma creatinine and T3 content, and insulin-like growth factor I (IGF-I), growth hormone receptor (GHR), uncoupling protein (UCP), adenine nucleotide translocase (ANT) and cytochrome c oxidase subunit III (COX III) mRNA expression in the liver and muscle of high (0.22 g/g) and low ...
Zeng X X Guangdong Institute of Microbiology, Guangzhou, China. Zhangjm926@126.com and - - 2014
Previous studies in our laboratory reported L-malate as a free radical scavenger in aged rats. To investigate the antioxidant mechanism of L-malate in the mitochondria, we analyzed the change in gene expression of two malate-aspartate shuttle (MAS)-related carried proteins (AGC, aspartate/glutamate carrier and OMC, oxoglutarate/malate carrier) in the inner mitochondrial ...
Sohn Yoon Ah YA Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, - - 2014
The P-element-induced wimpy testis (PIWI) protein, which associates with small non-coding RNAs, is responsible for maintaining the integrity of germ cells and stem cells. Thus, transcriptional regulation of PIWI is critical for its effective functional modulation. In this study, we identified the promoter region of the PIWI homolog in chicken ...
Kuroki Miho M Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo,Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, - - 2014
Nitrite reductase (NIR) catalyzes the reduction of nitrite to nitric oxide (NO) in the denitrification pathway. In Pseudomonas aeruginosa, expression of the gene encoding NIR (nirS)is induced by NO and is under control of the NO-sensing regulator DNR. BecauseDNR is under control of the oxygen-sensing regulator ANR, nirSis expressed only ...
Park Hyun-Jung HJ Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, 110-744, - - 2014
Parathyroid hormone-related protein (PTHrP) is known to induce the expression of receptor activator of NF-κB ligand (RANKL) in stromal cells/osteoblasts. However, the signaling pathways involved remain controversial. In the present study, we investigated the role of cAMP/protein kinase A (PKA) and calcineurin/NFAT pathways in PTHrP-induced RANKL expression in C2C12 and ...
Claycomb Kumiko I KI From the Department of Neuroscience, University of Connecticut Health Center, Farmington, Connecticut (KIC, PNW, KMJ, AMN, AWG, AVS, EB, SJC); Stem Cells and Regenerative Biology Program, Sanford-Burnham Institute, La Jolla, California (EYS); and Department of Anatomy and Cell Biology, University of Illinois, Chicago, Illinois - - 2014
Globoid cell leukodystrophy (GLD), or Krabbe disease, is a rare and often fatal demyelinating disease caused by mutations in the galactocerebrosidase (galc) gene that result in accumulation of galactosylsphingosine (psychosine). We recently reported that the extracellular matrix (ECM) protease, matrix metalloproteinase-3, is elevated in GLD and that it regulates psychosine-induced ...
Chen Fenglei F Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, - - 2014
CREBZF, also known as Zhangfei or SMILE, is a member of the CREB/ATF protein family. CREBZF has mainly been considered as a basic region-leucine zipper transcription factor that functions in coordination with other transcription factors and plays a role in latent HSV-1 infection, apoptosis and the mammalian endoplasmic reticulum stress ...
Imbriolo Jamie J Cardio-Metabolic Research Group (CMRG), Department of Physiological Sciences, Stellenbosch University, Stellenbosch 7600, South - - 2014
The cardiac isoform of acetyl-CoA carboxylase (ACCβ) produces malonyl-CoA, a potent inhibitor of mitochondrial fatty acid (FA) uptake. Higher ACCβ activity decreases FA utilization, potentially leading to intracellular myocardial lipid accumulation and insulin resistance (IR). Since increased hexosamine biosynthetic pathway (HBP) flux is linked to IR onset, we hypothesized that ...
Chen Jianping J Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, - - 2014
The fruit of Ziziphus jujuba Mill., known as jujube or Chinese date, is commonly consumed as health supplement or herbal medicine worldwide. To study the beneficial role of jujube in enhancing hematopoietic function, we investigated its roles on the expression of erythropoietin in cultured Hep3B human hepatocellular carcinoma cells. Application ...
Xu Yifeng Y Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Republic of - - 2014
Trimethylation of lysine 36 of histone H3 (H3K36me3) is found to be associated with various transcription events. In Arabidopsis, the H3K36me3 level peaks in the first half of coding regions, which is in contrast to the 3'-end enrichment in animals. The MRG15 family proteins function as 'reader' proteins by binding ...
Kruszka Katarzyna K Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Umultowska 89, 61-614 Poznan, - - 2014
Heat stress is one of the major abiotic factors that can induce severe plant damage, leading to a decrease in crop plant productivity. Despite barley being a cereal of great economic importance, few data are available concerning its thermotolerance mechanisms. In this work microRNAs (miRNAs) involved in heat stress response ...
Cordero-Alba Mar M Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Sevilla, - - 2014
SlrP is an E3 ubiquitin ligase that can be translocated into eukaryotic host cells by the two type III secretion systems that are expressed by Salmonella enterica serovar Typhimurium and are encoded in Salmonella pathogenicity islands 1 (SPI1) and 2 (SPI2), respectively. Expression of slrP and translocation of its product ...
Huang Kaipeng K Laboratory of Pharmacology & Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China; Department of Pharmacy, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, - - 2014
Sirt1 and nuclear factor-E2 related factor 2 (Nrf2)-anti-oxidant response element (ARE) anti-oxidative pathway play important regulatory roles in the pathological progression of diabetic nephropathy (DN) induced by advanced glycation-end products (AGEs). Polydatin (PD), a glucoside of resveratrol, has been shown to possess strong anti-oxidative bioactivity. Our previous study demonstrated that ...
Chu Guoming G Department of Clinical Genetics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China; Department of Medical Genetics, China Medical University, Shenyang, Liaoning, - - 2014
Nuclear receptor-binding SET domain-containing protein 1 (NSD1) has been proved to act as a histone methyltransferase and a transcription co-factor to regulate gene expression. However, the role of NSD1 in oxidative stress remains poorly understood. In the present study, we focused on the NSD1 regulation of antioxidant enzyme gene Glutathione ...
Kjeldsen Thea S B TS Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, - - 2014
Knowledge about the regulatory mechanisms of CTX-M β-lactamase-encoding genes in Escherichia coli is limited. The objectives of this study were to determine the growth response of CTX-M-1-producing E. coli exposed to cefotaxime and to investigate how blaCTX-M-1 expression at mRNA and protein levels is influenced by cefotaxime concentration, growth phase ...
Zhang Jiquan J Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China. Electronic address: - - 2014
Ferritin, a primary iron storage protein, plays an important role in iron homeostasis. In this study, a ferritin cDNA (EcFer) with a 516bp open reading frame (ORF) was obtained from Exopalaemon carinicauda (Holthuis) which encodes a 171 amino acid protein. At the 5-terminal untranslated region (5'-UTR), there is a complete ...
Uno K K Pharmaceutical Therapy and Neuropharmacology, University of Toyama, - - 2014
Many psychiatric disorders are caused by the abnormalities of the dopaminergic neuronal system. A novel N-acetyltransferase, Shati/Nat8l, was identified in the nucleus accumbens (NAc) of mice with methamphetamine (METH) treatment. Previously, we reported that suppression of Shati/Nat8l in the NAc enhanced METH-induced behavioral impairment via dopaminergic neuronal regulation by activation ...
Giuntoli Beatrice B Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, - - 2014
Transcriptional activation in response to hypoxia in plants is orchestrated by ethylene-responsive factor group VII (ERF-VII) transcription factors, which are stable during hypoxia but destabilized during normoxia through their targeting to the N-end rule pathway of selective proteolysis. Whereas the conditionally expressed ERF-VII genes enable effective flooding survival strategies in ...
Roy Kevin K Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California, United States of - - 2014
Bromodomain proteins are key regulators of gene expression. How the levels of these factors are regulated in specific environmental conditions is unknown. Previous work has established that expression of yeast Bromodomain factor 2 (BDF2) is limited by spliceosome-mediated decay (SMD). Here we show that BDF2 is subject to an additional ...
Gong Wenrong W Wenrong Gong, MD, Center for Molecular Medicine of Medical College/ Central Laboratory of the Affiliated Hospital, Hubei University of Arts and Science, Xiang Yang, Hubei, China - - 2014
This paper is aimed at to evaluate B7-H1 expression as induced by human cytomegalovirus (HCMV) in extravillous cytotrophoblast cell line HPT-8 and possible underlying mechanism. Real time PCR and flow cytometry were used to determine B7-H1 mRNA and protein before and after HCMV infection in HPT-8 cells. Western blot analysis ...
Espinoza-Lewis Ramón A RA Department of Cardiology, Boston Children's Hospital, Harvard Medical School, 320 Longwood Ave, Boston, MA, - - 2014
The molecular events that control cell fate determination in cardiac and smooth muscle lineages remain elusive. Myocardin is an important transcription co-factor that regulates cell proliferation, differentiation and development of the cardiovascular system. Here, we describe the construction and analysis of a dual Cre and Enhanced Green Fluorescent Protein (EGFP) ...
Li Qimeng Q Guanghua School of Stomatology and Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, - - 2014
Human dental pulp cells (hDPCs) can specifically generate reparative dentin under external stimuli, and numerous mechanisms are involved in their odontogenic differentiation process. Ten-eleven translocation 1 (TET1) is a recently discovered DNA dioxygenase that plays important roles in promoting DNA demethylation and transcriptional regulation. Although several studies regarding its effect ...
Tousi Samaneh E SE Molecular Genetics Department, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, - - 2014
The cardiac cells generation via stem cells differentiation is a promising approach to restore the myocardial infarction. Promoted by our primary bioinformatics analysis as well as some previously published data on potential role of hsa-miR-590-3p in cardiogenesis, we have tried to decipher the role of miR-590-5p during the course of ...
Tai Mi-Hsueh MH Department of Molecular Biology and Human Genetics, Tzu Chi University, Hualien, 970, - - 2014
Curcumin has been demonstrated as having numerous desirable characteristics, such as antioxidant, anti-inflammatory, and antiatherogenic activities. We report the hypocholesterolemic effect and molecular mechanism of curcumin. We found that curcumin enhanced LDL receptor (LDLR) level on the cell surface, as well as LDLR activity; however, LDLR transcription and mRNA stability ...
Glisic Branka B Molecular and Reproductive Toxicology Unit, Laboratory for Ecotoxicology, Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad, 21000, - - 2014
Glutathione-S-transferase (GST) superfamily consists of multiple members involved in xenobiotic metabolism. Expressional pattern of the GST isoforms in adult fish has been used as a biomarker of exposure to environmental chemicals. However, GST transcriptional responses vary across organs, thus requiring a cross-tissue examination of multiple mRNAs for GST profiling in ...
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