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Kraus Petra P Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY, 13699, - - 2015
With the advent of BAC recombineering techniques, transcriptional promoter and enhancer isolation studies have become much more feasible in zebrafish than in mouse given the easy access to large numbers of fertilized zebrafish eggs and offspring in general, the easy to follow ex-utero development of zebrafish, an overall less skill ...
Le XiangDong X Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, - - 2015
MicroRNAs have the ability to alter and regulate multiple genes, including RTK family members, making them an attractive approach for molecular therapeutic development. We use a pCDNA6.2-EmGFP-microRNA expression vector to overexpress individual mature microRNA and then transfer the expression cassette into a single, inducible lentiviral vector (pINDUCER20). We successfully use ...
Peralta-Zaragoza Oscar O Direction of Chronic Infections and Cancer, Research Center in Infection Diseases, National Institute of Public Health, Av. Universidad No. 655, Colonia Santa María Ahuacatitlán, Cuernavaca, Morelos, 62100, Mexico, - - 2015
RNA interference is a natural mechanism to silence post-transcriptional gene expression in eukaryotic cells in which microRNAs act to cleave or halt the translation of target mRNAs at specific target sequences. Mature microRNAs, 19-25 nucleotides in length, mediate their effect at the mRNA level by inhibiting translation, or inducing cleavage ...
Li An-Ying AY Department of Cardiology, The Third Xiangya Hospital of Central South University, No.138, Tongzipo Road, Yuelu District, Changsha, 410013, Hunan Province, - - 2014
Myocardial hypoxia is a major cause of cardiac dysfunction due to its triggering cell injury and apoptosis. Deregulated microRNAs and their roles in cardiomyocyte apoptosis have attracted much attention. miR-133a is among the most abundant of the miRNAs present in the normal heart, and significant changes in expression of miR-133a ...
Bebbere Daniela - - 2014
BackgroundThe sub-cortical maternal complex (SCMC), located in the subcortex of mouse oocytes and preimplantation embryos, is composed of at least four proteins encoded by maternal effect genes: OOEP, NLRP5/MATER, TLE6 and KHDC3/FILIA. The SCMC assembles during oocyte growth and was seen to be essential for murine zygote progression beyond the ...
Rana Satiander - - 2014
BackgroundPharmacological investigations position withanolides as important bioactive molecules demanding their enhanced production. Therefore, one of the pivotal aims has been to gain knowledge about complete biosynthesis of withanolides in terms of enzymatic and regulatory genes of the pathway. However, the pathway remains elusive at the molecular level. P450s monooxygenases play ...
Song Rui R Department of Surgery, University of Colorado Denver, Box C-320, 12700 E 19th Avenue, Aurora, CO, 80045, - - 2014
Biglycan accumulates in aortic valves affected by calcific aortic valve disease (CAVD), and soluble biglycan upregulates BMP-2 expression in human aortic valve interstitial cells (AVICs) via Toll-like receptor (TLR) 2 and induces AVIC pro-osteogenic reprogramming, characterized by elevated pro-osteogenic activities. We sought to identify the factors responsible for biglycan-induced pro-osteogenic ...
Byrne Leah C - - 2014
Alternative splicing of nucleoredoxin-like 1 (Nxnl1) results in 2 isoforms of the rod-derived cone viability factor. The truncated form (RdCVF) is a thioredoxin-like protein secreted by rods that promotes cone survival, while the full-length isoform (RdCVFL), which contains a thioredoxin fold, is involved in oxidative signaling and protection against hyperoxia. ...
Chalei Vladislava V MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United - - 2014
Many intergenic long noncoding RNA (lncRNA) loci regulate the expression of adjacent protein coding genes. Less clear is whether intergenic lncRNAs commonly regulate transcription by modulating chromatin at genomically distant loci. Here, we report both genomically local and distal RNA-dependent roles of Dali, a conserved central nervous system expressed intergenic ...
Oji Yusuke Y Department of Cancer Stem Cell Biology, Osaka University Graduate School of Medicine, Osaka, - - 2014
The Wilms' tumor gene WT1 is overexpressed in leukemia and various types of solid tumors and plays an oncogenic role in these malignancies. Alternative splicing at two sites yields four major isoforms, 17AA(+)KTS(+), 17AA(+)KTS(-), 17AA(-)KTS(+), and 17AA(-)KTS(-), and all the isoforms are expressed in the malignancies. However, among the four ...
Wang Li L Pathology and Laboratory Medicine, McAllister Heart Institute, University of North - - 2014
Rationale: Generation of induced cardiac myocytes (iCMs) directly from fibroblasts offers great opportunities for cardiac disease modeling and cardiac regeneration. A major challenge of iCM generation is the low conversion rate of fibroblasts to fully reprogrammed iCMs, which could in part be attributed to unbalanced expression of reprogramming factors Gata4 ...
Lu Zhipeng Z University of North - - 2014
Pre-mRNA splicing is a critical step in eukaryotic gene expression that contributes to proteomic, cellular and developmental complexity. Small nuclear (sn)RNAs are core spliceosomal components, however, the extent to which differential expression of snRNA isoforms regulates splicing is completely unknown. This is partly due to difficulties in the accurate analysis ...
Li Xi X Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing, 210095, People's Republic of - - 2014
The adequate supply of methyl donors is critical for the normal development of brain. The purpose of the present study was to investigate the effects of maternal betaine supplementation on hippocampal gene expression in neonatal piglets and to explore the possible mechanisms. Gestational sows were fed control or betaine-supplemented (3 g/kg) ...
Wang Chang-Hong CH Department of Psychiatry, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, 453002, Henan, - - 2014
Stressful life events especially the chronic unpredictable stress are the obvious precipitating factors of depression. The biological information transduction in cells plays an important role in the molecular biology mechanism of depression. Mitogen-activated protein kinase phosphatase-1 (MKP-1) regulates the cell physiological activity and involves in the adjustment of neural plasticity, ...
Lisachev P D PD Construction and Technological Institute of Computer Science, Siberian Division of the Russian Academy of Sciences, Novosibirsk, - - 2014
Expression of Bcl2 family genes was studied during the early phase of long-term potentiation in the CA1 field of rat hippocampal slices. The level of Bax mRNA and protein increased, while the content of Bcl2 mRNA and protein decreased 30 min after tetanization of the Schaffer collaterals. Our results suggest ...
Park Jun Hong JH Department of Pathology and Laboratory Medicine, Tulane University School of Medicine, New Orleans, Louisiana, - - 2014
White adipose tissue is important for mammalian energy homeostasis and metabolism. It was previously demonstrated that Ewing sarcoma gene (EWS) is essential for early classical brown fat lineage determination, but its role in white adipocyte differentiation is not known. Mouse embryonic fibroblasts (MEFs) lacking Ews and shRNA-mediated silencing of Ews ...
Li Dang-Dang DD College of Veterinary Medicine, Jilin University, Changchun, People's Republic of - - 2014
Ido2 is involved in tryptophan catabolism and immunity, but its physiological functions remain poorly understood. This study was undertaken to examine the expression and regulation of Ido2 gene in mouse uterus during the peri-implantation period. The results showed that Ido2 mRNA was highly expressed on day 4 of pregnancy and ...
Portela Valério M - - 2014
Although the various members of the fibroblast growth factor (FGF) family are generally mitotic, one member, FGF18, has been shown increase the rate of apoptotsis of ovarian granulosa cells. In the present study, we determined first whether granulosa cells express FGF18 and we then explored the mechanism through which FGF18 ...
Bégay Valérie V Department of Tumorigenesis and Cell Differentiation, Max Delbrueck Center for Molecular Medicine, Robert-Roessle-Str.10, 13125, Berlin, - - 2014
Two long and one truncated isoforms (termed LAP*, LAP, and LIP, respectively) of the transcription factor CCAAT enhancer binding protein beta (C/EBPβ) are expressed from a single intronless Cebpb gene by alternative translation initiation. Isoform expression is sensitive to mammalian target of rapamycin (mTOR)-mediated activation of the translation initiation machinery ...
Zhang Chunhong C 1 Department of Otorhinolaryngology, the First Affiliated Hospital of Wenzhou Medical College , Wenzhou, Zhejiang, China - - 2014
Abstract MicroRNAs can function as tumor suppressor miRNAs. Bcl-2 is an antiapoptotic gene overexpressed in many tumors, including nasopharyngeal carcinoma (NPC). It is reported that microRNA-15a (miR-15a) and microRNA-16-1 (miR-16-1) could act as bcl-2 inhibitors. To investigate their effects on NPC, the authors used recombinant lentiviral vector to upregulate the ...
Kubota Takeshi T Research Institute of Innovative Technology for the Earth (RITE), - - 2014
Shikimate can be utilized as the sole source of carbon and energy of Corynebacterium glutamicum. Although biosynthesis and degradation of shikimate are well characterized in C. glutamicum, the transport of shikimate has hardly been studied. A mutant strain deficient in cgR_2523 loses the ability to grow on shikimate as well ...
Shahzad Muhammad M a College of Veterinary Medicine , Huazhong Agricultural University , Wuhan 430070 , PR - - 2014
Tibial dyschondroplasia (TD) is an avian bone disorder of different aetiologies that may be associated with lameness. It is characterized by focal disruption of endochondral bone formation, with a lack of matrix proteolysis and an accumulation of non-mineralized avascular cartilage. The aim of this study was to determine the expression ...
Van Kleef Gerben A - - 2014
Despite a long-standing interest in the intrapersonal role of affect in persuasion, the interpersonal effects of emotions on persuasion remain poorly understood-how do one person's emotional expressions shape others' attitudes? Drawing on emotions as social information (EASI) theory (Van Kleef, 2009), we hypothesized that people use the emotional expressions of ...
Puget Nadine N CNRS UMR5089; IPBS (Institut de Pharmacologie et de Biologie Structurale); 205 route de Narbonne, BP 64182, 31077 Toulouse, France Université de Toulouse; UPS; IPBS; F-31077 Toulouse, - - 2014
The assembly of antigen receptor loci requires a developmentally regulated and lineage-specific recombination between variable (V), diversity (D) and joining (J) segments through V(D)J recombination. The process is regulated by accessibility control elements including promoters, insulators and enhancers. The IgH locus undergoes two recombination steps, D-JH, then VH-DJH, but it ...
Li Chen C Departments of Biochemistry and Molecular - - 2014
Pulmonary hypertension (PHT) is associated with high mortality in sickle cell anemia (SCA). Previously, we show elevated levels of placenta growth factor (PlGF) in SCA patients correlated with increased levels of the potent vasoconstrictor endothelin-1 (ET-1) and PHT. Moreover, PlGF induced the expression of ET-1 via HIF-1α. Herein, we show ...
Nagiah S S Discipline of Medical Biochemistry, University of KwaZulu Natal, Durban, South - - 2014
Chronic air pollution exposure during pregnancy can cause oxidative stress leading to adverse birth outcomes. The aim of this study was to assess and compare oxidative stress response in peripheral lymphocytes isolated from pregnant women from a highly industrialized locale (south Durban (SD); n = 50) and a control with ...
Maruyama Atsushi A Department of Stress Response Science, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, - - 2014
Recent studies have disclosed the function of enhancer RNAs (eRNAs), which are long non-coding RNAs transcribed from gene enhancer regions, in transcriptional regulation. However, it remains unclear whether eRNAs are involved in the regulation of human heme oxygenase-1 gene (HO-1) induction. Here, we report that multiple nuclear-enriched eRNAs are transcribed ...
Liu Chang Hong CH Department of Psychology, Bournemouth University, Talbot Campus, Fern Barrow, Poole, BH12 5BB, UK, - - 2014
Facial expression is a major source of image variation in face images. Linking numerous expressions to the same face can be a huge challenge for face learning and recognition. It remains largely unknown what level of exposure to this image variation is critical for expression-invariant face recognition. We examined this ...
Wilusz Jeremy E JE Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania 19104, USA; wilusz@mail.med.upenn.edu - - 2014
Production of multiple functional RNAs from a single primary transcript is an extremely efficient use of genetic information, although it complicates the ability of the cell to independently regulate the production of each RNA. For the case of small nucleolar RNAs (snoRNAs) encoded within introns of mRNA genes, Lykke-Andersen and ...
Itkonen Jaakko M - - 2014
BackgroundNeurotrophic factors influence survival, differentiation, proliferation and death of neuronal cells within the central nervous system. Human ciliary neurotrophic factor (hCNTF) has neuroprotective properties and is also known to influence energy balance. Consequently, hCNTF has potential therapeutic applications in neurodegenerative, obesity and diabetes related disorders. Clinical and biological applications of ...
Patel Utsav A UA Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India, PIN- - - 2014
Myostatin (MSTN) is a secreted growth factor that negatively regulates skeletal muscle mass and thus strategies to block myostatin signaling pathway have been extensively pursued to increase the muscle mass in livestock. Here, we report a lentiviral-vector based delivery of shRNA to disrupt myostatin expression into goat fetal fibroblasts (GFFs) ...
Piatek Agnieszka A Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi - - 2014
Targeted genomic regulation is a powerful approach to accelerate trait discovery and development in agricultural biotechnology. Bacteria and archaea use clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) regulatory systems for adaptive molecular immunity against foreign nucleic acids introduced by invading phages and conjugative plasmids. The type II ...
Liu Te T Shanghai Tenth People's Hospital, Medical School, Tongji University, Shanghai 200072, P.R. - - 2014
Human‑induced pluripotent (iPS) cells can be induced to differentiate into osteoblasts, but the process is inefficient and time‑consuming. Previous studies indicated a close association between the expression of Runx2 and osteoblast differentiation, and established that the transcriptional activation of the Runx2 gene was closely associated with histone acetylation. microRNA‑449a (miR‑449a) ...
Luo Biping B 1 College of Veterinary Medicine, Nanjing Agricultural University , Jiangsu, 210095, China - - 2014
Abstract The purpose of this study was to investigate the effects of the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) on transgene expression and development of porcine transgenic cloned embryos, specifically focusing on effects derived from TSA-treated donor cells or TSA-treated reconstructed embryos. The results showed that TSA treatment on ...
Fros Jelke J JJ Laboratory of Virology, Wageningen University, The - - 2014
The unfolded protein response (UPR) is a cellular defense mechanism against high concentrations of misfolded protein in the endoplasmic reticulum (ER). In the presence of misfolded proteins, ER-transmembrane proteins PERK and IRE1α become activated. PERK phosphorylates eIF2α leading to a general inhibition of cellular translation, while the expression of transcription ...
Shaikhali Jehad J Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences SLU, 901 83, Umeå, Sweden, - - 2014
In response to environmental light signals, gene expression adjustments play an important role in regulation of photomorphogenesis. LHCB2.4 is among the genes responsive to light signals, and its expression is regulated by redox-regulated members of G-group bZIP transcription factors. The biochemical interrelations of GBF1-interacting protein 1 (GIP1) and the G-group ...
Zhang Kuixing K Departments of - - 2014
Chromogranin A (CHGA) is coreleased with catecholamines from secretory vesicles in adrenal medulla and sympathetic axons. Genetic variation in the CHGA 3'-region has been associated with autonomic control of circulation, hypertension, and hypertensive nephropathy, and the CHGA 3'-untranslated region (3'-UTR) variant C+87T (rs7610) displayed peak associations with these traits in ...
Andersson Karl-Magnus - - 2014
Nematode-trapping fungi are soil-living fungi that capture and kill nematodes using special hyphal structures called traps. They display a large diversity of trapping mechanisms and differ in host preferences. To provide insights into the genetic basis for this variation, we compared the transcriptome expressed by three species of nematode-trapping fungi ...
McCue Andrea D AD Department of Molecular Genetics, The Ohio State University, Columbus, OH, - - 2014
Transposable elements (TEs) generate mutations and chromosomal instability when active. To repress TE activity, eukaryotic cells evolved mechanisms to both degrade TE mRNAs into small interfering RNAs (siRNAs) and modify TE chromatin to epigenetically inhibit transcription. Since the populations of small RNAs that participate in TE post-transcriptional regulation differ from ...
Li Chen C University of California, United - - 2014
Endothelin-1, a potent vasoconstrictor, plays an important role in pulmonary hypertension (PH) in sickle cell disease (SCD). Our previous studies show higher levels of placenta growth factor (PlGF), secreted by erythroid precursor cells, correlate with increased plasma levels of endothelin-1 (ET-1) and other functional markers of PH in SCD. PlGF ...
Umemura Yasuhiro Y Department of Physiology and Systems Bioscience, Kyoto Prefectural University of Medicine, Kyoto 602-8566, - - 2014
The circadian clock in mammalian cells is cell-autonomously generated during the cellular differentiation process, but the underlying mechanisms are not understood. Here we show that perturbation of the transcriptional program by constitutive expression of transcription factor c-Myc and DNA methyltransferase 1 (Dnmt1) ablation disrupts the differentiation-coupled emergence of the clock ...
Guo Jia J Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, P.R. - - 2014
The process of epithelial‑mesenchymal transition (EMT) is essential for the proliferation and metastasis of tumor cells. Our previous study demonstrated that the expression of gangliotetraosylceramide (Gg4) and the transcription of UDP‑Gal: β1,3-galactosyltransferase‑4 (β3GalT4), a gene which controls the expression of Gg4, are reduced during transforming growth factor (TGF)‑β‑induced EMT in ...
Akhtar Nahid N Department of Anatomy/Neurobiology, Northeast Ohio Medical University, 4209 State Route 44, Rootstown, Ohio, - - 2014
Objective. Hedgehog (Hh) signaling has recently been associated with cartilage degradation in osteoarthritis (OA). As interleukin-1β (IL-1β) is a critical mediator of OA pathogenesis, here we determined whether IL-1β induces the expression of sonic hedgehog (SHH) and its regulation by microRNAs in human chondrocytes. Methods. SHH protein expression in human ...
Kromm Helena H University of - - 2014
The development of volitional emotion regulation of expression was examined with a modified disappointing gift paradigm and strategy for coding children's expressions. Forty-nine boys and 49 girls aged 4, 6, and 8 were motivated to volitionally deceive an observer by false smiling, regardless of whether they received an attractive, unattractive, ...
Yoshimura Sayaka S Department of Neurodevelopmental Psychiatry, Habilitation and Rehabilitation, Graduate School of Medicine, Kyoto University, 54 Shogoin-Kawaharacho, Sakyo, Kyoto, 606-8507, Japan, - - 2014
Previous electromyographic studies have reported that individuals with autism spectrum disorders (ASD) exhibited atypical patterns of facial muscle activity in response to facial expression stimuli. However, whether such activity is expressed in visible facial mimicry remains unknown. To investigate this issue, we videotaped facial responses in high-functioning individuals with ASD ...
Biswal Manas R MR Molecular Genetics and Microbiology, University of Florida, College of Medicine, 1200 Newel Drive, R1-281, Gainesville Florida, 32601, United - - 2014
Purpose: Müller cells, the major glial cell in the retina, play a significant role in retinal neovascularization in response to tissue hypoxia. We previously designed and tested a vector using a hypoxia-responsive domain and a glial fibrillary acidic protein (GFAP) promoter to drive green fluorescent protein (GFP) expression in Müller ...
Ma H H Department of Endocrinology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, PR - - 2014
The insulin receptor substrate 1 (IRS1) promotes bone formation via osteoblast proliferation mediated by PI3K/Akt signaling. A reduction in NFκB activity in osteoblasts results in an increase in bone formation. The NFκB signaling pathway leads to increased expression of BAX, which contributes to osteoblast apoptosis. The purpose of this study ...
Yin Qing-Fei QF State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, - - 2014
Many long noncoding RNAs (lncRNAs) are constrained to the nucleus to exert their functions. However, commonly used vectors that were designed to express mRNAs have not been optimized for the study of nuclear RNAs. We reported recently that sno-lncRNAs are not capped or polyadenylated but rather are terminated on each ...
Hallé Catherine C Département de Biologie,Faculté des Sciences, Université de - - 2014
Feeding flaxseed to dairy cows can modulate gene expression and PG synthesis in the uterus at the time of peri-implantation. The objectives of the present study were to determine which flaxseed components are responsible for these effects and how different endometrial cell types are affected. We evaluated the effects of ...
Kim Yoon-Jin YJ Department of Biology, Research Institute for Basic Science, Kyung Hee University, Seoul, 130-701, - - 2014
Preadipocyte factor 1 (Pref-1), also known as a delta-like 1 protein, is a transmembrane and secreted protein containing the epidermal growth factor-like repeat. Pref-1 inhibits adipocyte differentiation by activating the ERK1/2 pathway. MicroRNAs, a new class of small noncoding RNAs of 20-24 nucleotides, act as negative regulators of gene expression ...
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