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Engelund Morten Buch MB Department of Biology, University of Southern Denmark, Campusvej 55, 5230, Odense M, Denmark, - - 2014
Most vertebrate nephrons possess an inherited ability to secrete fluid in normal or pathophysiological states. We hypothesized that renal aquaporin expression and localization are functionally regulated in response to seawater and during smoltification in Atlantic salmon and thus reflect a shift in renal function from filtration towards secretion. We localized ...
Chuang Chao-Tang CT Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, - - 2014
The advanced glycation end‑product (AGE)‑receptor for AGE (RAGE) axis induces transforming growth factor‑β (TGF‑β) expression, cell hypertrophy and increases extracellular matrices that are indicated in the pathogenesis of diabetic nephropathy (DN). RAGE binds to numerous ligands besides AGE, including S100B. In the present study, the roles of S100B in high ...
Kakimoto Tetsuhiro T Safety Research Laboratories, Research Division, Mitsubishi Tanabe Pharma Corporation, Toda-shi 335-8505, Saitama, Japan. Electronic address: - - 2014
Podocytes are an essential component of the renal glomerular filtration barrier, their injury playing an early and important role in progressive renal dysfunction. This makes quantification of podocyte marker immunoreactivity important for early detection of glomerular histopathological changes. Here we have specifically applied a state-of-the-art automated computational method of glomerulus ...
Zhou Xiangjun X Department of Urology, Renmin Hospital of Wuhan University, - - 2014
Renal fibrosis is mainly characterized by activation and proliferation of interstitial fibroblasts and by excessive synthesis and accumulation of extracellular matrix (ECM) components, including fibronectin (FN) and collagen. This study investigated the effects of curcumin on proliferation of renal interstitial fibroblasts and their underlying mechanisms in vivo and in vitro. ...
Park Gunhyuk - - 2014
Heat shock increases skin temperature during sun exposure and some evidence indicates that it may be involved in skin ageing. The antioxidant response mediated by the transcription factor NF-E2-related factor 2 (Nrf2) is a critically important cellular defense mechanism that serves to limit skin ageing. We investigated the effects of ...
Briffa Jessica F JF Centre for Chronic Disease Prevention and Management, College of Health and Biomedicine, Victoria University, St Albans, VIC 3021, Australia; Department of Physiology, The University of Melbourne, Parkville, VIC 3010, - - 2014
Increased leptin concentrations observed in obesitycan lead to proteinuria, suggesting that leptin may play a role in obesity-related kidney disease. Obesity reduces activation of AMP-activated protein kinase (AMPK) and increases transforming growth factor-β1 (TGF-β1) expression in the kidney, leading to albuminuria. Thus we investigated if elevated leptin altered AMPK and ...
Tillgren Viveka V Lund University, - - 2014
The constitution and biophysical properties of extracellular matrices can dramatically influence cellular phenotype during development, homeostasis, or pathogenesis. These effects can be signaled through a differentially regulated assembly of collagen fibrils, orchestrated by a family of collagen-associated Small Leucine-Rich Proteins, SLRPs. In this report, we describe the tissue-specific expression and ...
Wang Xuepeng X China-Japan Union Hospital, Jilin University, Changchun, People's Republic of - - 2015
As the newest identified member of the matrix metalloproteinase (MMP) family, the expression pattern and function of epilysin (MMP-28) are still not well understood. Although epilysin was found to play an evolutionarily conserved role in neural development, the expression and function of epilysin in malignant glioma are unknown. Therefore, the ...
Song Ping P Respiratory Department, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. - - 2014
Idiopathic pulmonary fibrosis (IPF) is a fatal lung disease characterized by expansion of the fibroblast and myofibroblast population and extracellular matrix deposition. Although the pathogenic mechanisms of IPF remain to be fully elucidated, there is emerging evidence that fibroblasts and myofibroblasts may be derived partially from alveolar epithelial cells by ...
Cao Kun - - 2014
IntroductionTo investigate if decreased histone deacetylase 4 (HDAC4) is associated with human osteoarthritis (OA) cartilage degeneration by releasing HDAC4 inhibition of runt-related transcription factor-2 (Runx2) resulting in increase of OA cartilage degeneration related genes.MethodsThe mRNA and protein levels of HDAC4, Runx2, matrix metalloproteinase (MMP)-13, Indian hedgehog (Ihh) and type X ...
Hartwell Ryan R University of British Columbia, Surgery , 818 W 10th Ave , Rm 4200 , Vancouver, Canada , V5Z 1M9 , 604-675-8862 ; - - 2014
Wound repair requires a sequential series of biological events that begins with the deposition of a temporary scaffold within which cells can repair the skin. Without a scaffold, repair is essentially impossible. Aberrant wound healing, such as hypertrophic scarring or non-healing has a tremendous burden on healthcare and quality of ...
Ogiwara Katsueki - - 2014
We previously reported that the serine protease plasmin plays a role in follicle rupture during ovulation in the teleost medaka. In this study, we showed that urokinase-type plasminogen activator 1 (Plau1) is a physiological activator of plasminogen. Morphological analyses revealed that in the preovulatory follicle, plau1 mRNA was detected in ...
Berardi Anna C AC UOC Immunohematology and Transfusion Medicine Laboratories, Laboratory of Stem Cells, Spirito Santo Hospital, Pescara, - - 2014
we previously demonstrated the presence of high levels of thyroid hormones (THs) receptors isoforms in healthy tendons, their protective action during tenocyte apoptosis, and the capability to enhance tenocyte proliferation in vitro. In the present study we tested the ability of THs to influence ECM protein tenocyte secretion in an ...
Pomari Elena E Centre for Skin Sciences, School of Life Sciences University of Bradford, Bradford, United Kingdom; Comparative Endocrinology Laboratory, Department of Biology, University of Padova, Padua, Italy; - - 2014
Peripheral intracrine sex steroid synthesis from adrenal precursors dehydroepiandrosterone (DHEA) and DHEA-sulfate has evolved in humans. We sought to establish if there are differences in intracrine, paracrine, and endocrine regulation of sex steroids by primary cultures of human skin epidermal keratinocytes and dermal fibroblasts. Microarray analysis identified multifunctional genes modulated ...
Liu Jingjing J 1Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, China, 2Department of Respiratory Medicine, Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science & Technology, Wuhan, China, 3Pharmaceutical School, Central South University, Changsha, China, 4Department of Nephrology Medicine, Xiangya Hospital, Central South University, Changsha, China, and 5Key cite of National Clincial Research Center for Respiratory - - 2014
Caveolin-1 plays an important role in the pathogenesis of idiopathic pulmonary fibrosis. We previously showed that fluorofenidone (FD), a novel pyridine agent, can attenuate bleomycin (BLM)-induced experimental pulmonary fibrosis and restore the production of caveolin-1. In this study, we explore mainly whether caveolin-1 plays a critical role in the anti-pulmonary ...
Reich Adi A Bone and Extracellular Matrix Branch, NICHD, NIH, Bethesda, MD, - - 2014
Context: Patients with type V osteogenesis imperfecta (OI) are heterozygous for a dominant IFITM5 c.-14C>T mutation, which adds 5 residues to the N-terminus of BRIL, a transmembrane protein expressed in osteoblasts. Type V OI skeletal findings include hyperplastic callus formation, ossification of the forearm interosseous membrane and dense metaphyseal bands. ...
Amara Suneetha S Department of Medicine, Mercy Hospital, St Louis, MO, - - 2014
The pro-inflammatory cytokines, tumor necrosis factor (TNF)-α, and interleukin (IL)-17, have been implicated in the pathogenesis of liver fibrosis. In this study, we investigated the role of TNFα and IL-17 toward induction of profibrotic factor, periostin. HepG2 cells were cultured and treated with inflammatory cytokines, TNFα and IL-17. Computational promoter ...
Roh Jung Hyeob JH Division of Infectious Diseases, Department of Internal Medicine, The University of Texas, Medical School, Houston, TX, USA Center for the Study of Emerging and Re-emerging Pathogens, The University of Texas, Medical School, Houston, TX, - - 2014
Expression of ace (Adhesin to collagen of Enterococcus faecalis), encoding a virulence factor in endocarditis and urinary tract infection models, has been shown to increase under certain conditions such as in the presence of serum, bile salts, urine, collagen, and at 46°C. However, the mechanism of ace/Ace regulation under different ...
Fabiś Jaroslaw J Department of Arthroscopy, Minimally Invasive Surgery and Sports Traumatology, Medical University of Łódź, Poland; FMC Private Medical Centre Łódź, Poland. Electronic address: - - 2014
Type I collagen proin pro-in expression in a damaged supraspinatus tendon is thought to be dependent on the distance from the edge of the tear and the local expression of pro-inflammatory, anti-proliferative, and pro-proliferative cytokines. The study evaluates the expression of type I collagen, pro-inflammatory interleukin (IL) 1β, anti-proliferative interferon-γ ...
Yan Yong Y Neurosurgery Research Institution of Shanghai, Department of Neurosurgery, ChangZheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, 200003, - - 2014
To study the expression profiles of lncRNA and mRNA in glioblastoma multiforme (GBM) and to find potential core genes in the pathogenesis of this high malignant disease. Agilent Microarray (Arrystar v2.0) was used to detect the expressions of 33,045 lncRNAs and 30,215 coding transcripts in 5 GBM and 5 normal brain ...
Halbwirth Florian F Center for Regenerative Medicine and Orthopedics, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500, Krems, Austria, - - 2014
The treatment of cartilage defects with matrix-embedded autologous chondrocytes is a promising method to support the repair process and to foster reconstitution of full functionality of the joint. Human osteoarthritic chondrocytes were harvest from nine different patients (mean ± SD age 68 ± 8 years) who underwent total knee replacement. The chondrocytes were embedded after ...
Chinzei Nobuaki N Department of Orthopedic Surgery, Kobe University Graduate School of Medicine, Kobe, Hyogo 650‑0017, - - 2014
Endochondral ossification at the growth plate is regulated by a number of factors and hormones. The cyclin‑dependent kinase inhibitor p21 has been identified as a cell cycle regulator and its expression has been reported to be essential for endochondral ossification in vitro. However, to the best of our knowledge, the function ...
Zhu Lubing L Department of Dermatology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, - - 2014
The most dominant feature of systemic sclerosis (SSc) is fibrosis, which is caused by overproduction of collagen by fibroblasts. 2-Methoxyestradiol (2-ME) has exhibited disease-modifying activity in animal models of rheumatoid arthritis and autoimmune encephalomyelitis and inhibitory effect in cell proliferation and collagen synthesis. Therefore, we hypothesized that 2-ME may exhibit ...
Wang Zhi Cong ZC Department of Orthopedic Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. - - 2014
Icariin (ICA), a Traditional Chinese Medicine, has been demonstrated to be a promoting compound for extracellular matrix synthesis and gene expression of chondrocytes. However, whether ICA can act as a substitute for or cooperate with growth factors to directly promote stable chondrogenesis of bone marrow mesenchymal stem cells (BMSCs) remains ...
Oefner C M CM Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom. Electronic address: - - 2014
Extracellular matrix proteins play a crucial role in influencing the invasion of trophoblast cells. However the role of collagens and collagen type IV (col-IV) in particular at the implantation site is not clear. Immunohistochemistry was used to determine the distribution of collagen types I, III, IV and VI in endometrium ...
Xu Huihui H State Key Laboratory of Reproductive Medicine, Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Pathophysiology - - 2014
Diabetic nephropathy (DN) is one of the most common complications associated with diabetes and characterized by renal microvascular injury along with accelerated synthesis of extracellular matrix proteins causing tubulointerstitial fibrosis. Production of type I collagen, the major component of extracellular matrix, is augmented during renal fibrosis after chronic exposure to ...
Nakajima Fukami F Department of Anesthesiology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8519, - - 2014
The deletion mutation of exon 4 in surfactant protein C (SP-C), a lung surfactant protein, has been identified in parent-child cases of familial interstitial pneumonia. It has been shown that this mutation induces endoplasmic reticulum (ER) stress. Synoviolin is an E3 ubiquitin ligase that is localized to the ER and is an important factor ...
Li Zhi-Qiang ZQ Department of Pediatric Cardiac Surgery Center, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. - - 2014
The present study aimed to investigate the effect of C‑type natriuretic peptide (CNP) on the function of cardiac fibroblasts (CFs). Western blotting was used to investigate the expression of myofibroblast marker proteins: α‑smooth muscle actin (α‑SMA), extra domain‑A fibronectin, collagen I and collagen III, and the activity of extracellular signal‑regulated kinase 1/2 (ERK1/2). Immunofluorescence ...
McCulloch Laura J LJ Diabetes and Obesity Research Group, University of Exeter Medical School, Exeter, - - 2014
Fibrosis of adipose tissue (AT) increases AT rigidity, reduces its expandability and contributes to metabolic dysfunction. Collagen type VI, alpha3 (COL6A3) encodes one subunit of a fibrotic extracellular matrix (ECM) protein highly expressed in rodent AT. Knock-out of collagen VI in rodent AT led to a significant improvement in metabolic ...
Zhao Jiajia J Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Department of Stomatology, Wuhan, HuBei, China ; - - 2014
Macrophage-stimulating protein (MSP), an important cytokine with multiple functions, is highly expressed in adipose mesenchymal stem cells-conditioned medium (ASC-CM). ASCs can effectively promote wound healing through paracrine mechanism, suggesting that MSP may play a critical role in wound healing. Through binding to its receptor, RON, it can activate epithelial cells ...
Kim So Young SY Major in Biomedical Science, Department of Medicine, Graduate School, Chung-Ang University, Seoul, Republic of - - 2014
Skin aging is induced through complex biological processes in human skin caused by proteolysis of collagen and elastin, two structural proteins of the dermal extracellular matrix (ECM). Collagen and elastin degradation can induce the expression of matrix metalloproteinases (MMPs), as well as that of a family of zinc-dependent endopeptidases that ...
Shen Xiao X Department of Plastic and Burn Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. - - 2014
Adipose‑derived stem cells (ADSCs) are known to secrete various cytokines, which affect fibroblast function through paracrine effects. In the present study, the paracrine effects of ADSCs on the function and senescence of young and aged human dermal fibroblasts (HDFs) were investigated in vitro. ADSCs and HDFs were isolated from healthy donors ...
Meng Delong - - 2014
BackgroundGlioblastoma (GBM) is the most common and lethal intracranial malignancy in adults, with dismal prognosis despite multimodal therapies. Tectonic family member 1 (TCTN1) is a protein involved in a diverse range of developmental processes, yet its functions in GBM remain unclear. This study aims to investigate expression profile, prognostic value ...
Wang Weiwei W Department of Ophthalmology, Xi'an No. 4 Hospital, School of Medicine, Xi'an Jiao Tong University, Xi'an, Shaanxi Province, China, - - 2014
Heat shock protein 47 (HSP47), a collagen-specific molecular chaperone, is involved in the biosynthesis and secretion of procollagen. Recent studies have shown a close association between increased HSP47 and excessive accumulation of collagens in various fibrotic diseases. This study was conducted to evaluate whether HSP47 plays a role in conjunctival ...
Hu Bin B Department of Human Molecular Genetics, Virginia Commonwealth - - 2014
The oncogene AEG-1 (MTDH) is highly expressed in glioblastoma multiforme (GBM) and many other types of cancer, where it activates multiple signaling pathways that drive proliferation, invasion, angiogenesis, chemoresistance, radioresistance and metastasis. AEG-1 activates the Akt signaling pathway and Akt and c-Myc are positive regulators of AEG-1 transcription, generating a ...
Ponticos Markella M Centre for Rheumatology & Connective Tissue Diseases, University College London Medical School, London, - - 2014
Objectives: The excessive deposition of extracellular matrix including collagen type I is a key aspect in the pathogenesis of connective tissue diseases such as Scleroderma (SSc). To further our understanding of the mechanisms governing the dysregulation of type I collagen production in SSc, we investigated the role of the activator ...
Furukawa Fumiya F University of Miyazaki - - 2014
Regulation of plasma K(+) levels in narrow ranges is vital to vertebrate animals. Since seawater (SW) teleosts are loaded with excess K(+), they constantly excrete K(+) from the gills. However, the K(+) regulatory mechanisms in freshwater (FW)-acclimated teleosts are still unclear. We aimed to identify the possible K(+) regulatory mechanisms ...
Oncel Torun Z Z Department of Restorative Dentistry and Endodontics, Gulhane Military Medical Academy, Ankara, - - 2014
To evaluate the cytotoxicity and mineralization effects of iRoot BP in human dental pulp cells (hDPCs) and to compare them with those of white mineral trioxide aggregate (WMTA). hDPCs were exposed to prepared dilutions (1:1-1:10) of the test materials. Cell viability was evaluated using the XTT assay after incubation periods ...
Schäfer Georgia G Division of Medical Biochemistry, Faculty of Health Sciences, University of Cape Town, South Africa; Institute of Infectious Disease and Molecular Medicine (IDM), University of Cape Town, South - - 2014
The fibrillar collagen scaffold of the extracellular matrix provides a structural framework for cells in tissues and regulates intercellular communication; its disregulation has been associated with tumour development and progression. Previous work has shown that expression of type I collagen, the most abundant mammalian extracellular matrix protein, is decreased in ...
Adachi Hiroaki H Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Nagoya, - - 2014
Although ultraviolet (UV) rays cause premature aging of human skin, which is called photoaging, its detailed mechanisms are not known. Stratifin (SFN), a member of the 14-3-3 protein family, is secreted by keratinocytes on human skin, and has an effect on gene expression in other cells. In this study, the ...
Forriol Francisco F University San Pablo - CEU, School of Medicine, Madrid, Spain. Electronic address: - - 2014
This study analysed the influence of bone morphogenetic protein-7 (BMP-7) on cells and meniscal structure. The effect of treatment with BMP-7 was assessed in vitro and in vivo in lesions in the avascular area of the meniscus. Cells were extracted from the outer and inner part of eight menisci of ...
Liu Dan D Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing, Jiangsu, - - 2014
Albuminuria is not only an important marker of chronic kidney disease but also a crucial contributor to tubulointerstitial inflammation (TIF). In this study, we determined whether activation of the Nlrp3 inflammasome is involved in albuminuria induced-TIF and the underlying mechanisms of inflammasome activation by mitochondrial reactive oxygen species (mROS). We ...
Lu Zhenhui Z The Medical and Scientific Research Center, Guangxi Medical University, Nanning 530021, China; Research Center for Regenerative Medicine, Guangxi Medical University, Nanning 530021, - - 2014
Chondrocyte based therapy is promising to treat symptomatic chondral and osteochondral lesions. Growth factors to accelerate the proliferation and retain the phenotype of chondrocytes in vitro are imperative. However, the high cost and rapid degradation of growth factors limited their further application. Therefore, it is significant to find substitutes that ...
Malecaze Francois F department of ophthalmology, Purpan hospital, 1 place du Dr Baylac, toulouse, 31059, - - 2014
Purpose: To characterize the expression of the bone morphogenetic protein-1 (BMP-1)/tolloid-like proteinases (collectively called BTPs), which include BMP-1, mammalian Tolloid (mTLD), mammalian tolloid-like 1 (mTLL-1) and 2 (mTLL-2), as well as the associated proteins procollagen C-proteinase enhancers (PCPE-1 and -2), in corneal scarring. Methods: Using a mouse full-thickness corneal excision ...
Bordji Karim K CNRS, UMR 6301 ISTCT, CERVOxy group, GIP Cyceron, F-14074, Caen, France; CEA, DSV/I2BM, UMR 6301 ISTCT, F-14074, Caen, France; Université de Basse-Normandie, UMR 6301 ISTCT, F-14074, Caen, France; Normandie Univ, - - 2014
Glioblastoma multiforme (GBM) is the deadliest form of primary brain cancer. Several reports indicated aberrant levels of βIII-tubulin (βIII-t) in human GBM. βIII-t overexpression was linked to increasing malignancy in glial tumors and described to determine the onset of resistance to chemotherapy. Furthermore, a linkage was suggested between the induction ...
He Xiaori X Department of Neonatology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. - - 2014
Intrauterine growth retardation (IUGR) can affect kidney development, leading to reduction in glomerular number. However, the associated cellular and molecular mechanisms have not been fully elucidated. This study investigated cell apoptosis and Bcl-2 and Bax expression in the kidney of IUGR pups. The IUGR model was established in pregnant rats ...
Qin Zhaoping Z Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan, - - 2014
The dermal compartment of human skin is largely composed of dense collagen-rich fibrils, which provide structural and mechanical support. Skin dermal fibroblasts, the major collagen-producing cells, are interact with collagen fibrils to maintain cell spreading and mechanical force for function. A characteristic feature of aged human skin is fragmentation of ...
Hall Michael R MR Department of Vascular Biology and Therapeutics Program, Yale University School of Medicine, New Haven, Connecticut - - - 2014
The venous limb of arteriovenous fistulae (AVF) adapts to the arterial environment by dilation and wall thickening; however, the temporal regulation of the expression of extracellular matrix (ECM) components in the venous limb of the maturing AVF has not been well characterized. We used a murine model of AVF maturation ...
Ma Jing J Medical College, Eye Institute of Xiamen University, South Xiang'an Road, Xiamen, Fujian, 361102, - - 2014
Purpose:To investigate the exact mechanism by which keratocytes promote corneal neovascularization. Methods:The expression of MMP13, CD146, VEGFa, VEGFc, VEGFr2 and VEGFr3 by normal and alkali-burned rat corneas was determined by qRT-PCR and/or Western blot analysis, or in situ hybridization. Corneal neovascularization was observed under slit lamp microscope and evaluated by ...
Huang Youqun - - 2014
MicroRNAs have been demonstrated to play an important role in the pathogenesis of diabetic nephropathy (DN). In this study, we investigated both the repertoire of miRNAs in the kidneys of patients with DN and their potential regulatory role in inflammation-mediated glomerular endothelial injury. The miRNA expression profiling of the renal ...
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