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Pomari Elena E Centre for Skin Sciences, School of Life Sciences University of Bradford, Bradford, United Kingdom; Comparative Endocrinology Laboratory, Department of Biology, University of Padova, Padua, Italy; - - 2014
Peripheral intracrine sex steroid synthesis from adrenal precursors dehydroepiandrosterone (DHEA) and DHEA-sulfate has evolved in humans. We sought to establish if there are differences in intracrine, paracrine, and endocrine regulation of sex steroids by primary cultures of human skin epidermal keratinocytes and dermal fibroblasts. Microarray analysis identified multifunctional genes modulated ...
Liu Jingjing J 1Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, China, 2Department of Respiratory Medicine, Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science & Technology, Wuhan, China, 3Pharmaceutical School, Central South University, Changsha, China, 4Department of Nephrology Medicine, Xiangya Hospital, Central South University, Changsha, China, and 5Key cite of National Clincial Research Center for Respiratory - - 2014
Caveolin-1 plays an important role in the pathogenesis of idiopathic pulmonary fibrosis. We previously showed that fluorofenidone (FD), a novel pyridine agent, can attenuate bleomycin (BLM)-induced experimental pulmonary fibrosis and restore the production of caveolin-1. In this study, we explore mainly whether caveolin-1 plays a critical role in the anti-pulmonary ...
Reich Adi A Bone and Extracellular Matrix Branch, NICHD, NIH, Bethesda, MD, - - 2014
Context: Patients with type V osteogenesis imperfecta (OI) are heterozygous for a dominant IFITM5 c.-14C>T mutation, which adds 5 residues to the N-terminus of BRIL, a transmembrane protein expressed in osteoblasts. Type V OI skeletal findings include hyperplastic callus formation, ossification of the forearm interosseous membrane and dense metaphyseal bands. ...
Roh Jung Hyeob JH Division of Infectious Diseases, Department of Internal Medicine, The University of Texas, Medical School, Houston, TX, USA Center for the Study of Emerging and Re-emerging Pathogens, The University of Texas, Medical School, Houston, TX, - - 2014
Expression of ace (Adhesin to collagen of Enterococcus faecalis), encoding a virulence factor in endocarditis and urinary tract infection models, has been shown to increase under certain conditions such as in the presence of serum, bile salts, urine, collagen, and at 46°C. However, the mechanism of ace/Ace regulation under different ...
Yan Yong Y Neurosurgery Research Institution of Shanghai, Department of Neurosurgery, ChangZheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, 200003, - - 2014
To study the expression profiles of lncRNA and mRNA in glioblastoma multiforme (GBM) and to find potential core genes in the pathogenesis of this high malignant disease. Agilent Microarray (Arrystar v2.0) was used to detect the expressions of 33,045 lncRNAs and 30,215 coding transcripts in 5 GBM and 5 normal brain ...
Halbwirth Florian F Center for Regenerative Medicine and Orthopedics, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500, Krems, Austria, - - 2014
The treatment of cartilage defects with matrix-embedded autologous chondrocytes is a promising method to support the repair process and to foster reconstitution of full functionality of the joint. Human osteoarthritic chondrocytes were harvest from nine different patients (mean ± SD age 68 ± 8 years) who underwent total knee replacement. The chondrocytes were embedded after ...
Chinzei Nobuaki N Department of Orthopedic Surgery, Kobe University Graduate School of Medicine, Kobe, Hyogo 650‑0017, - - 2014
Endochondral ossification at the growth plate is regulated by a number of factors and hormones. The cyclin‑dependent kinase inhibitor p21 has been identified as a cell cycle regulator and its expression has been reported to be essential for endochondral ossification in vitro. However, to the best of our knowledge, the function ...
Wang Zhi Cong ZC Department of Orthopedic Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. - - 2014
Icariin (ICA), a Traditional Chinese Medicine, has been demonstrated to be a promoting compound for extracellular matrix synthesis and gene expression of chondrocytes. However, whether ICA can act as a substitute for or cooperate with growth factors to directly promote stable chondrogenesis of bone marrow mesenchymal stem cells (BMSCs) remains ...
Xu Huihui H State Key Laboratory of Reproductive Medicine, Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Pathophysiology - - 2014
Diabetic nephropathy (DN) is one of the most common complications associated with diabetes and characterized by renal microvascular injury along with accelerated synthesis of extracellular matrix proteins causing tubulointerstitial fibrosis. Production of type I collagen, the major component of extracellular matrix, is augmented during renal fibrosis after chronic exposure to ...
Nakajima Fukami F Department of Anesthesiology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8519, - - 2014
The deletion mutation of exon 4 in surfactant protein C (SP-C), a lung surfactant protein, has been identified in parent-child cases of familial interstitial pneumonia. It has been shown that this mutation induces endoplasmic reticulum (ER) stress. Synoviolin is an E3 ubiquitin ligase that is localized to the ER and is an important factor ...
Li Zhi-Qiang ZQ Department of Pediatric Cardiac Surgery Center, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. - - 2014
The present study aimed to investigate the effect of C‑type natriuretic peptide (CNP) on the function of cardiac fibroblasts (CFs). Western blotting was used to investigate the expression of myofibroblast marker proteins: α‑smooth muscle actin (α‑SMA), extra domain‑A fibronectin, collagen I and collagen III, and the activity of extracellular signal‑regulated kinase 1/2 (ERK1/2). Immunofluorescence ...
McCulloch Laura J LJ Diabetes and Obesity Research Group, University of Exeter Medical School, Exeter, - - 2014
Fibrosis of adipose tissue (AT) increases AT rigidity, reduces its expandability and contributes to metabolic dysfunction. Collagen type VI, alpha3 (COL6A3) encodes one subunit of a fibrotic extracellular matrix (ECM) protein highly expressed in rodent AT. Knock-out of collagen VI in rodent AT led to a significant improvement in metabolic ...
Zhao Jiajia J Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Department of Stomatology, Wuhan, HuBei, China ; - - 2014
Macrophage-stimulating protein (MSP), an important cytokine with multiple functions, is highly expressed in adipose mesenchymal stem cells-conditioned medium (ASC-CM). ASCs can effectively promote wound healing through paracrine mechanism, suggesting that MSP may play a critical role in wound healing. Through binding to its receptor, RON, it can activate epithelial cells ...
Kim So Young SY Major in Biomedical Science, Department of Medicine, Graduate School, Chung-Ang University, Seoul, Republic of - - 2014
Skin aging is induced through complex biological processes in human skin caused by proteolysis of collagen and elastin, two structural proteins of the dermal extracellular matrix (ECM). Collagen and elastin degradation can induce the expression of matrix metalloproteinases (MMPs), as well as that of a family of zinc-dependent endopeptidases that ...
Shen Xiao X Department of Plastic and Burn Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. - - 2014
Adipose‑derived stem cells (ADSCs) are known to secrete various cytokines, which affect fibroblast function through paracrine effects. In the present study, the paracrine effects of ADSCs on the function and senescence of young and aged human dermal fibroblasts (HDFs) were investigated in vitro. ADSCs and HDFs were isolated from healthy donors ...
Meng Delong - - 2014
BackgroundGlioblastoma (GBM) is the most common and lethal intracranial malignancy in adults, with dismal prognosis despite multimodal therapies. Tectonic family member 1 (TCTN1) is a protein involved in a diverse range of developmental processes, yet its functions in GBM remain unclear. This study aims to investigate expression profile, prognostic value ...
Wang Weiwei W Department of Ophthalmology, Xi'an No. 4 Hospital, School of Medicine, Xi'an Jiao Tong University, Xi'an, Shaanxi Province, China, - - 2014
Heat shock protein 47 (HSP47), a collagen-specific molecular chaperone, is involved in the biosynthesis and secretion of procollagen. Recent studies have shown a close association between increased HSP47 and excessive accumulation of collagens in various fibrotic diseases. This study was conducted to evaluate whether HSP47 plays a role in conjunctival ...
Hu Bin B Department of Human Molecular Genetics, Virginia Commonwealth - - 2014
The oncogene AEG-1 (MTDH) is highly expressed in glioblastoma multiforme (GBM) and many other types of cancer, where it activates multiple signaling pathways that drive proliferation, invasion, angiogenesis, chemoresistance, radioresistance and metastasis. AEG-1 activates the Akt signaling pathway and Akt and c-Myc are positive regulators of AEG-1 transcription, generating a ...
Ponticos Markella M Centre for Rheumatology & Connective Tissue Diseases, University College London Medical School, London, - - 2014
Objectives: The excessive deposition of extracellular matrix including collagen type I is a key aspect in the pathogenesis of connective tissue diseases such as Scleroderma (SSc). To further our understanding of the mechanisms governing the dysregulation of type I collagen production in SSc, we investigated the role of the activator ...
Furukawa Fumiya F University of Miyazaki - - 2014
Regulation of plasma K(+) levels in narrow ranges is vital to vertebrate animals. Since seawater (SW) teleosts are loaded with excess K(+), they constantly excrete K(+) from the gills. However, the K(+) regulatory mechanisms in freshwater (FW)-acclimated teleosts are still unclear. We aimed to identify the possible K(+) regulatory mechanisms ...
Oncel Torun Z Z Department of Restorative Dentistry and Endodontics, Gulhane Military Medical Academy, Ankara, - - 2014
To evaluate the cytotoxicity and mineralization effects of iRoot BP in human dental pulp cells (hDPCs) and to compare them with those of white mineral trioxide aggregate (WMTA). hDPCs were exposed to prepared dilutions (1:1-1:10) of the test materials. Cell viability was evaluated using the XTT assay after incubation periods ...
Schäfer Georgia G Division of Medical Biochemistry, Faculty of Health Sciences, University of Cape Town, South Africa; Institute of Infectious Disease and Molecular Medicine (IDM), University of Cape Town, South - - 2014
The fibrillar collagen scaffold of the extracellular matrix provides a structural framework for cells in tissues and regulates intercellular communication; its disregulation has been associated with tumour development and progression. Previous work has shown that expression of type I collagen, the most abundant mammalian extracellular matrix protein, is decreased in ...
Adachi Hiroaki H Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Nagoya, - - 2014
Although ultraviolet (UV) rays cause premature aging of human skin, which is called photoaging, its detailed mechanisms are not known. Stratifin (SFN), a member of the 14-3-3 protein family, is secreted by keratinocytes on human skin, and has an effect on gene expression in other cells. In this study, the ...
Liu Dan D Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing, Jiangsu, - - 2014
Albuminuria is not only an important marker of chronic kidney disease but also a crucial contributor to tubulointerstitial inflammation (TIF). In this study, we determined whether activation of the Nlrp3 inflammasome is involved in albuminuria induced-TIF and the underlying mechanisms of inflammasome activation by mitochondrial reactive oxygen species (mROS). We ...
Lu Zhenhui Z The Medical and Scientific Research Center, Guangxi Medical University, Nanning 530021, China; Research Center for Regenerative Medicine, Guangxi Medical University, Nanning 530021, - - 2014
Chondrocyte based therapy is promising to treat symptomatic chondral and osteochondral lesions. Growth factors to accelerate the proliferation and retain the phenotype of chondrocytes in vitro are imperative. However, the high cost and rapid degradation of growth factors limited their further application. Therefore, it is significant to find substitutes that ...
Malecaze Francois F department of ophthalmology, Purpan hospital, 1 place du Dr Baylac, toulouse, 31059, - - 2014
Purpose: To characterize the expression of the bone morphogenetic protein-1 (BMP-1)/tolloid-like proteinases (collectively called BTPs), which include BMP-1, mammalian Tolloid (mTLD), mammalian tolloid-like 1 (mTLL-1) and 2 (mTLL-2), as well as the associated proteins procollagen C-proteinase enhancers (PCPE-1 and -2), in corneal scarring. Methods: Using a mouse full-thickness corneal excision ...
Bordji Karim K CNRS, UMR 6301 ISTCT, CERVOxy group, GIP Cyceron, F-14074, Caen, France; CEA, DSV/I2BM, UMR 6301 ISTCT, F-14074, Caen, France; Université de Basse-Normandie, UMR 6301 ISTCT, F-14074, Caen, France; Normandie Univ, - - 2014
Glioblastoma multiforme (GBM) is the deadliest form of primary brain cancer. Several reports indicated aberrant levels of βIII-tubulin (βIII-t) in human GBM. βIII-t overexpression was linked to increasing malignancy in glial tumors and described to determine the onset of resistance to chemotherapy. Furthermore, a linkage was suggested between the induction ...
He Xiaori X Department of Neonatology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. - - 2014
Intrauterine growth retardation (IUGR) can affect kidney development, leading to reduction in glomerular number. However, the associated cellular and molecular mechanisms have not been fully elucidated. This study investigated cell apoptosis and Bcl-2 and Bax expression in the kidney of IUGR pups. The IUGR model was established in pregnant rats ...
Qin Zhaoping Z Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan, - - 2014
The dermal compartment of human skin is largely composed of dense collagen-rich fibrils, which provide structural and mechanical support. Skin dermal fibroblasts, the major collagen-producing cells, are interact with collagen fibrils to maintain cell spreading and mechanical force for function. A characteristic feature of aged human skin is fragmentation of ...
Hall Michael R MR Department of Vascular Biology and Therapeutics Program, Yale University School of Medicine, New Haven, Connecticut - - - 2014
The venous limb of arteriovenous fistulae (AVF) adapts to the arterial environment by dilation and wall thickening; however, the temporal regulation of the expression of extracellular matrix (ECM) components in the venous limb of the maturing AVF has not been well characterized. We used a murine model of AVF maturation ...
Ma Jing J Medical College, Eye Institute of Xiamen University, South Xiang'an Road, Xiamen, Fujian, 361102, - - 2014
Purpose:To investigate the exact mechanism by which keratocytes promote corneal neovascularization. Methods:The expression of MMP13, CD146, VEGFa, VEGFc, VEGFr2 and VEGFr3 by normal and alkali-burned rat corneas was determined by qRT-PCR and/or Western blot analysis, or in situ hybridization. Corneal neovascularization was observed under slit lamp microscope and evaluated by ...
Huang Youqun - - 2014
MicroRNAs have been demonstrated to play an important role in the pathogenesis of diabetic nephropathy (DN). In this study, we investigated both the repertoire of miRNAs in the kidneys of patients with DN and their potential regulatory role in inflammation-mediated glomerular endothelial injury. The miRNA expression profiling of the renal ...
Park Su-Hyun SH Research Institute of Biomedical Engineering and Department of Medicine, Catholic University of Daegu School of Medicine, Daegu 705-718, Republic of - - 2014
Renal fibrosis is characterized by the excessive accumulation of extracellular matrix (ECM) proteins such as type I collagen, fibronectin, and by the increased expression of PAI-1. This study evaluated the anti-fibrotic effect of bee venom and its major compounds (melittin and apamin) on TGF-β-induced pro-fibrotic gene expression. Bee venom and ...
Liu Shuangxin S Department of Nephrology - - 2014
Abstract Podocyte damage and loss together have an important role in the pathogenesis and progression of glomerulonephritis. Glomerulonephritis patients and healthy controls were enrolled in this study. Biochemical, clinical and experimental procedures included measurement of total urinary protein, renal biopsy and gene expression analysis of the receptor activator of NF-kappaB ...
Jia Qingliang Q Shaanxi R&D Centre of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, 710069, People's Republic of - - 2014
Human-like collagen (HLC) is a novel biomedical material with promising applications. Usually, insoluble HLC was formed due to over-expression. In order to improve the production of soluble HLC, the effective chaperone proteins and their mediation roles on HLC were clarified. Trigger factor (TF) pathway with low specificity and high binding ...
Nho Richard Seonghun RS University of Minnesota - - 2014
Idiopathic Pulmonary Fibrosis (IPF) is a lethal and progressive lung disease characterized by persistent (myo)fibroblasts and the relentless accumulation of collagen matrix. Unlike normal lung fibroblasts, IPF lung fibroblasts have suppressed forkhead box O3a (FoxO3a) activity which allows them to expand in this diseased environment. microRNA-96 (miR-96) has recently been ...
Thakkar Dipti D Nuffield Department of Orthopedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, - - 2014
Abstract There is increasing evidence for a progressive extracellular matrix change in rotator cuff disease progression. Directly surrounding the cell is the pericellular matrix, where assembly of matrix aggregates typically occurs making it critical in the response of tendon cells to pathological conditions. Studies in animal models have identified type ...
Hu Weihua W Department of Orthopaedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, P.R. - - 2014
The structural backbone of extracellular matrix in cartilage is the collagen fibril, which is mainly composed of type II collagen. A measurable increase in type II collagen denaturation and degradation has been found in early Osteoarthritis (OA). Pro-inflammatory cytokine such as TNF-α produced in OA cartilage induced the expression of ...
Makino Takamitsu T Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto, 860-8556, - - 2014
Background: Localized scleroderma (LSc) exhibits fibrosis of the skin and subcutaneous tissue. LSc shows an excessive deposition of type 1 collagen. Objectives: To elucidate the mechanism of type 1 collagen overexpression in LSc, we investigated the epigenetics, focusing on microRNA (miRNA). Materials & Methods: miRNA expression profile was determined by ...
Li Jin J Department of Orthopedics Surgery, The Second Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310000, - - 2014
In this study, we investigated the effects of hinokitiol on matrix metalloproteinase (MMP)-1, -3, -13, collagen type II (Col2a1) and β-catenin expressions in rat chondrocytes induced by interleukin-1β and in an experimental rat model induced by intra-articular injection of mono-iodoacetate (MIA) into the knee. Chondrocytes were cultured from the articular ...
Huang Yen-Sung YS Institute of Biomedical Sciences, Academia Sinica, - - 2014
Membranous nephropathy (MN), a type of glomerular nephritis, is the most common cause of nephrotic syndrome in human adults. Changes in gene expression as a result of epigenetic dysregulation through long noncoding RNAs (lncRNAs) are increasingly being recognized as important factors in disease. Using an experimental MN mouse model, we ...
Zhu Huang H Department of Ophthalmology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, - - 2014
This study investigated the effects of extremely low frequency electromagnetic fields (ELF-EMFs) on human fetal scleral fibroblasts (HFSFs). HFSFs were subjected to 50 Hz artificial ELF-EMFs generated by Helmholtz coils with 0.1, 0.2, 0.5, and 1.0 mT field intensities for 6 to 48 h. The viability and factors involved in ...
Wong May Yw MY Kolling Institute of Medical - - 2014
Semicarbazide-sensitive amine oxidase (SSAO) is an enzyme known for its dual function in mediating inflammation and reactive oxygen species production. However, the role of SSAO inhibitors in limiting kidney fibrosis is unclear. We aimed to determine the effectiveness of a SSAO inhibitor (PXS-4728A) as an antifibrotic agent using a 7-day ...
Xiao Zhicheng Z Beijing AnZhen Hospital, Capital Medical University; The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing, - - 2014
Kidney fibrosis is a common feature of chronic kidney disease (CKD). A recent study suggests that abnormal Notch signaling activation contributes to the development of renal fibrosis. However, the molecular mechanism that regulates this process remains unexplored. Unilateral ureteral obstruction (UUO) or sham-operated C57BL6 mice (aged 10 weeks) were randomly ...
Fang Ling L School of Pharmacy, Anhui Medical University, Mei Shan Road, Hefei, Anhui Province 230032, China; Institute for Liver Diseases of Anhui Medical University, Mei Shan Road, Hefei, Anhui Province 230032, China; Key Laboratory of Anti-inflammatory and Immune Medicine, Anhui Medical University, Ministry of Education, Mei Shan Road, Hefei, Anhui Province 230032, China; The First Affiliated Hospital of Anhui Medical University, Mei Shan Road, Hefei, Anhui Province 230032, China. Electronic address: - - 2014
Transdifferentiation of hepatic stellate cells (HSCs) into myofibroblasts plays a critical role in the development of liver fibrosis, since myofibroblasts are the key cell type responsible for excessive deposition of ECM proteins. Transient receptor potential melastatin 7 (TRPM7), a non-selective cation channel with protein serine/threonine kinase activity, has been demonstrated ...
Wang Lin L Center for Tissue Engineering and Regenerative Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, - - 2014
Cell invasion through basement membrane (BM) occurs in many physiological and pathological contexts. MIG-10, the Caenorhabditiselegans Lamellipodin (Lpd), regulates diverse biological processes. Its function and regulation in cell invasive behavior remain unclear. Using anchor cell (AC) invasion in C. elegans as an in vivo invasion model, we have previously found ...
Wang Feng F Department of Nephrology and Rheumatology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, - - 2014
Background/Aims : Renalase is a recently discovered, kidney-specific monoamine oxidase that metabolizes circulating catecholamines. These findings present new insights into hypertension and chronic kidney diseases. Previous data demonstrated that renalase was mainly secreted from proximal tubules which could be evoked by catecholamines. The purpose of this study is to investigate ...
Song Yu Seok YS Department of Biochemistry, Chung-Ang University College of Medicine, Seoul 156-756, - - 2014
In this study we investigated the effects of fucoidan on the proliferation of fibroblasts and the reconstruction of a skin equivalent (SE). Fucoidan significantly stimulated the proliferation of CCD-25Sk human fibroblasts and Western blot analysis demonstrated that fucoidan markedly increased the expression of cyclin D1 and decreased the expression of ...
Weng Xiaping X College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. - - 2014
Achyranthes bidentata polysaccharides (ABPS) are the active components of Radix Achyranthis Bidentatae (AB), which has been extensively used in Traditional Chinese medicine (TCM) in the treatment of osteoarthritis (OA). Our previous study provided evidence that ABPS regulated the G1/S transition to promote chondrocyte proliferation. However, the precise mechanisms involved remain to ...
Viale-Bouroncle Sandra S Department of Cranio and Maxillofacial Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, D-93053 Regensburg, - - 2014
Dental follicle cells (DFCs) are the genuine precursors of alveolar osteoblasts. Previous studies suggested that collagen I supports the osteogenic differentiation of DFCs. This study investigated the effect of collagen I on the osteogenic differentiation of human DFCs. We modified the cell culture surface with collagen I and evaluated the ...
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