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Yang-Hartwich Yang Y Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, - - 2015
p53 is a key transcriptional mediator that controls the expression of hundreds of target genes necessary to maintain cellular homeostasis and genome integrity. An important cellular function that is dependent on p53 transcriptional activity is apoptosis or programmed cell death. Indeed, inhibition of p53 transcriptional activity is often observed in ...
Gagnon David D Laboratory of Molecular Virology, Institut de Recherches Cliniques de Montréal, 110 Pine Avenue West, Montreal, QC, Canada, H2W - - 2015
A subset of human papillomaviruses (HPVs), known as the high-risk types, are the causative agents of cervical cancer and other malignancies of the anogenital region and oral mucosa. The capacity of these viruses to induce cancer and to immortalize cells in culture relies in part on a critical function of ...
Sun Yan Y Department of Pediatrics, Peking University First Hospital, Xi-An Men Street No. 1, West District, Beijing, 100034, - - 2014
The study was designed to investigate whether H2S could upregulate expression of KATP channels in vascular smooth muscle cells (VSMCs), and by this mechanism enhances vasorelaxation in spontaneously hypertensive rats (SHR). Blood pressure, vascular structure, and vasorelaxation were analyzed. Plasma H2S was detected using polarographic sensor. SUR2B and Kir6.1 expressions ...
Han Arim - - 2014
Here we report that the H3K9 demethylase KDM3B represses transcription of the angiogenesis regulatory gene, ANGPT1. Negative regulation of ANGPT1 by KDM3B is independent of its Jumanji (JmjC) domain-mediated H3K9 demethylase activity. We demonstrate that KDM3B downregulates ANGPT1 via interaction with SMRT, and suggest that the repressor complex is formed ...
Kostka Gra┼╝yna G Department of Toxicology and Risk Assessment, National Institute of Public Health-National Institute of Hygiene, Chocimska 24, 00-791, Warsaw, - - 2014
The aim of the study was to investigate the early effect of acute dichlorodiphenyltrichloroethane (DDT) exposure on the methylation status of the promoter region of two tumor suppressor genes: p53 and p16(INK4a) (p16) in rat liver. We analyzed their transcript and protein expression profiles concurrently with the examination of transcriptional ...
Armoni M M Institute of Endocrinology, Diabetes and Metabolism, Rambam Medical Center, and Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, - - 2014
Being a key-factor in glucose homeostasis, PPARγ transcriptional activity (TA) is of high importance. However, its mediation by ligands and post-translational modifications in insulin target tissues are unclear. We investigated effects of rosiglitazone (Rg) and sumoylation on PPARγ-TA by overexpressing expression vectors and promoter-reporters for PPARγ1 and PPARγ2 in primary ...
Bellutti Florian F Children's Cancer Research Institute, Vienna, - - 2014
Adenoviruses encode a set of highly abundant miRNAs (mivaRNAs), which are generated by Dicer-mediated cleavage of the larger non-coding virus-associated RNAs (VA RNAs) I and II. We performed deep RNA sequencing to thoroughly investigate the relative abundance of individual single strands of mivaRNA isoforms in human A549 cells lytically infected ...
Gotoh Tetsuya T Integrated Cellular Responses Laboratory, Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, - - 2014
Circadian period proteins influence cell division and death by associating with checkpoint components, although their mode of regulation has not been firmly established. hPer2 forms a trimeric complex with hp53 and its negative regulator Mdm2. In unstressed cells, this association leads to increased hp53 stability through blocking Mdm2-dependent ubiquitination and ...
Zhang Xiao-Wei XW Department of Medical Oncology, Cancer Hospital of Fudan University, 270 Dong An Road, Shanghai, 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, - - 2014
The tumor suppressor p53 plays an important role in tumorigenesis. Besides inducing apoptosis, it regulates cellular senescence, which constitutes an important barrier to tumorigenesis. The mechanism of regulation of cellular senescence by p53 and its downstream pathway are poorly understood. Here, we report that the ubiquitin domain-containing 1 gene (UBTD1), ...
Giovarelli Matteo M Gene Expression Regulation Laboratory, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Azienda Ospedaliera Universitaria San Martino-IST, 16132 Genoa, - - 2014
Long noncoding RNAs (lncRNAs) interact with protein factors to regulate different layers of gene expression transcriptionally or posttranscriptionally. Here we report on the functional consequences of the unanticipated interaction of the RNA binding protein K homology-type splicing regulatory protein (KSRP) with the H19 lncRNA (H19). KSRP directly binds to H19 ...
Li Geng G Institute of Molecular Medicine, Peking University, Beijing 100871, - - 2014
Phosphatidylinositol 3 kinases (PI3Ks) play a pivotal role in vascular physiology and pathophysiology. We aimed to investigate the role of p55γ, a regulatory subunit of PI3Ks, in vascular smooth muscle cell (VSMC) proliferation and neointimal formation. We identified p55γ as an important factor that suppresses VSMC proliferation and injury-evoked neointimal ...
Gao J J 1] Department of Gastrointestinal Oncology, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, China [2] Institute of Digestive Disease, Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, CUHK Shenzhen Research Institute, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, Hong - - 2014
Although surgery remains the mainstay of curative treatment for colorectal cancer (CRC), many patients still have high chance to experience disease relapse. It is therefore imperative to identify prognostic markers that can help predict the clinical outcomes of CRC. Aberrant microRNA expression holds great potential as diagnostic and prognostic biomarker ...
Ji Hong H Department of Paediatrics, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. - - 2014
The differentiation of fibroblasts, which are promoted by transforming growth factor-β (TGF-β)/Smad, is involved in the process of pulmonary fibrosis. The Rho/Rho-associated coiled-coil-forming protein kinase (Rock) pathway may regulate the fibroblast differentiation and myofibroblast expression of α-smooth muscle actin (α-SMA), however, the mechanism is not clear. The aim of the ...
Johnson Karen E KE H.M. Bligh Cancer Research Laboratories, Department of Microbiology and Immunology, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois, United States of - - 2014
Interferon-γ inducible factor 16 (IFI16) is a multifunctional nuclear protein involved in transcriptional regulation, induction of interferon-β (IFN-β), and activation of the inflammasome response. It interacts with the sugar-phosphate backbone of dsDNA and modulates viral and cellular transcription through largely undetermined mechanisms. IFI16 is a restriction factor for human cytomegalovirus ...
Yang Xiaoxue X School of Life Science and Engineering, Harbin Institute of Technology, Harbin, - - 2014
To reduce expression of gene products not required under stress conditions, eukaryotic cells form large and complex cytoplasmic aggregates of RNA and proteins (stress granules; SGs), where transcripts are kept translationally inert. The overall composition of SGs, as well as their assembly requirements and regulation through stress-activated signaling pathways remain ...
Paydas Saime S Department of Nephrology, Faculty of Medicine, Cukurova University, Adana, - - 2014
Secondary hyperparathyroidism (SH) is major problem in chronic renal failure. There are studies to examine proliferation and apoptosis associated biomarkers expressions in parathyroid lesions to reveal specific features. In this study, we evaluated the expression of some growth factors and their receptors in parathyroid gland of patients with SH or ...
Takata Takanobu T Medical Research Institute of Kanazawa Medical University, Ishikawa, - - 2014
Saikokeishito (TJ-10) is a Kampo (traditional Japanese herbal) medicine, clinically used for hundreds of years in East Asia. Among its various mechanisms elucidated so far, TJ-10 inhibits the production of transforming growth factor-β1 (TGF-β1) and development of pancreatic fibrosis in vivo. Oxidative damage of normal human dermal fibroblasts (NHDFs) in ...
Keshet R R Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, - - 2014
YES-associated protein (YAP) is a central transcription coactivator that functions as an oncogene in a number of experimental systems. However, under DNA damage, YAP activates pro-apoptotic genes in conjunction with p73. This program switching is mediated by c-Abl (Abelson murine leukemia viral oncogene) via phosphorylation of YAP at the Y357 ...
Hsu Sung-Po SP Department of Physiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, - - 2014
We previously demonstrated that progesterone (P4) up-regulates p53 expression in human umbilical venous endothelial cells (HUVEC) through P4 receptor (PR) activation of extra-nuclear signaling pathways. However, the involvement of nuclear PR in P4-increased p53 expression is still unclear. Here, the molecular mechanism underlying PR-regulated p53 expression in HUVEC was investigated. ...
Parnell Emily J EJ University of Utah, United - - 2014
The RTS1 gene encodes a subunit of the PP2A phosphatase that regulates cell cycle progression. Ace2 and Swi5 are cell cycle regulated transcription factors, and we recently showed that phosphorylation of Ace2 and Swi5 is altered in an rts1 mutant. Here we examine expression of Ace2 and Swi5 target genes ...
Bonde Marie Mi MM Biozentrum, University of Basel, Klingelbergstrasse 50/70, 4056 Basel, - - 2014
Splicing reactions generally combine high speed with accuracy. However, some of the pre-mRNAs escape the nucleus with a retained intron. Intron retention can control gene expression and increase proteome diversity. We calculated the escape rate for the yeast PTC7 intron and pre-mRNA. This prediction was facilitated by the observation that ...
Anshu Ashish A Department of Biological Sciences, UW-Milwaukee, - - 2014
A signaling network called the unfolded protein response (UPR) resolves the protein-folding defects in the endoplasmic reticulum (ER) from yeast to human. In yeast Saccharomyces cerevisiae, the UPR activation involves (1) aggregation of the ER-resident kinase/RNase Ire1 to form an Ire1-focus, (2) targeting HAC1 pre-mRNA towards the Ire1-focus that cleaves ...
Redondo-Muñoz Javier J Departments of Immunology and - - 2014
The nuclear envelope (NE) forms a barrier between the nucleus and the cytosol that preserves genomic integrity. The nuclear lamina and nuclear pore complexes (NPC) are NE components that regulate nuclear events through interaction with other proteins and DNA. Defects in nuclear lamina are associated with the development of laminopathies. ...
Neitemeier Sandra S Institut für Pharmakologie und Klinische Pharmazie, Fachbereich Pharmazie, Philipps-Universität Marburg, Karl-von-Frisch-Straße 1, 35032, Marburg, - - 2014
Impaired mitochondrial integrity and function are key features of intrinsic death pathways in neuronal cells. Therefore, key regulators of intrinsic death pathways acting upstream of mitochondria are potential targets for therapeutic approaches of neuroprotection. The tumor suppressor p53 is a well-established regulator of cellular responses towards different kinds of lethal ...
Hock Andreas K AK CR-UK Beatson Institute, United - - 2014
USP42 is a deubiquitylating enzyme that can target p53 and contribute to the stabilization of p53 in response to stress. We now show that USP42 can also regulate transcription independently of p53. USP42 co-localized with RNA pol II in nuclear foci, bound to histone H2B and deubiquitylated H2B. Depletion of ...
O'Loghlen A A 1] Cell Proliferation Group, Epigenetic Section, MRC Clinical Sciences Centre, Imperial College London, Hammersmith Campus London, UK [2] Molecular Oncology Laboratory, CRUK London Research Institute, London, - - 2014
The nuclear receptor NR2E1 (also known as TLX or tailless) controls the self-renewal of neural stem cells (NSCs) and has been implied as an oncogene which initiates brain tumors including glioblastomas. Despite NR2E1 regulating targets like p21(CIP1) or PTEN we still lack a full explanation for its role in NSC ...
Nikonorova Inna A IA Department of Cellular and Molecular Pharmacology, Rutgers Robert Wood Johnson Medical School, Piscataway, NJ 08854, - - 2014
TRPM7 is an essential and ubiquitous channel-kinase regulating cellular influx of Mg(2+). Although TRPM7 mRNA is highly abundant, very small amount of the protein is detected in cells, suggesting post-transcriptional regulation of trpm7 gene expression. We found that TRPM7 mRNA 5'-leader contains two evolutionarily conserved upstream open reading frames that ...
Moriuchi Takanobu T Graduate School of Life Science, University of Hyogo, Koto, Kamigori, Hyogo, 678-1297, - - 2014
Mutation of the lamin A gene (LMNA) causes a diverse range of diseases referred to as laminopathies. Because most laminopathies have a dominant inheritance pattern and progress gradually, cultured cells stably expressing mutant lamin A at the same level as endogenous wild-type cells are required for chronological analysis. In this ...
Vreeland Amanda C AC Case Western Reserve University, United - - 2014
Cellular retinoic acid-binding protein 2 (CRABP2) potently suppresses the growth of various carcinomas but the mechanism(s) that underlie this activity remain incompletely understood. CRABP2 displays two distinct functions: the classical function of this protein is to directly deliver retinoic acid (RA) to RAR, a nuclear receptor activated by this hormone, ...
Hua Brian L BL Massachusetts Institute of - - 2014
The mechanisms that underlie metazoan DNA replication initiation, especially the connection between transcription and replication origin activation, are not well understood. To probe the role of transcription in origin activation, we exploited a specific replication origin in Drosophila melanogaster follicle cells, ori62, which coincides with the yellow-g2 transcription unit and ...
Hallenborg Philip P University of Southern Denmark, - - 2014
Adipocyte differentiation is orchestrated by the ligand-activated nuclear receptor PPARγ. Endogenous ligands comprise oxidized derivatives of arachidonic acid and structurally similar polyunsaturated fatty acids. Although expression of PPARγ peaks in mature adipocytes, ligands are produced primarily at the onset of differentiation. Concomitant with agonist production murine fibroblasts undergo two rounds ...
Lichius Alexander A Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, - - 2014
Trichoderma reesei is a model for investigating the regulation of (hemi-)cellulase gene expression. Cellulases are formed adaptively, and the transcriptional activator XYR1 and the carbon catabolite repressor CRE1 are main regulators of their expression. We quantified the nucleo-cytoplasmic shuttling dynamics of GFP-fusion proteins of both transcription factors under cellulase and ...
Zhou X X Department of Biochemistry & Molecular Biology, Tulane Cancer Center; Tulane University School of Medicine; New Orleans, Louisiana, - - 2014
Over the past decade, a number of ribosomal proteins (RPs) have been found to have a role in activating the tumor suppressor p53 by directly binding to MDM2 and impeding its activity toward p53. Herein, we report that RPL5 and RPL11 can also enhance the transcriptional activity of a p53 ...
Soares Joana J REQUIMTE, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira n.° 164, 4050-313, Porto, Portugal; Laboratório de Microbiologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira n.° 164, 4050-313, Porto, - - 2014
One of the most appealing targets for anticancer treatment is the p53 tumor suppressor protein. In half of human cancers, this protein is inactivated due to endogenous negative regulators such as MDM2. Actually, restoring the p53 activity, particularly through the inhibition of its interaction with MDM2, is considered a valuable ...
Chuang Tsai-Der TD Department of Obstetrics and Gynecology, Harbor-UCLA Medical Center and LA Biomed, Torrance, CA, - - 2014
Uterine leiomyosarcoma is a relatively rare malignancy with high mortality due to metastasis and chemoresistance. Leiomyosarcomas share similar morphological characteristics with leiomyomas which are considered to have the potential of transformation into leiomyosarcoma. Accumulated evidence suggests that microRNAs acting as regulators of gene expression at the posttranscriptional level play key ...
R Costa Joana J Centre for Haematology, Department of Medicine, Hammersmith Hospital, Imperial College London, London, United - - 2014
A rare point mutation in the core promoter -270GC-rich box of PIGM, a housekeeping gene, disrupts binding of the generic transcription factor (TF) Sp1 and causes inherited glycosylphosphatidylinositol (GPI) deficiency (IGD). We show that while PIGM mRNA levels and surface GPI expression in IGD B cells are low, GPI expression ...
Koh Dong-In DI Brain Korea 21 Plus Project for Medical Sciences, Department of Biochemistry and Molecular Biology, Severance Biomedical Research Institute, Yonsei University School of Medicine, SeoDaeMoon-Ku, Seoul 120-752, - - 2014
An unresolved issue in genotoxic stress response is identification of induced regulatory proteins and how these activate tumor suppressor p53 to determine appropriate cell responses. Transcription factor KAISO was previously described to repress transcription following binding to methylated DNA. In this study, we show that KAISO is induced by DNA ...
Kiriyama Yuuka Y Department of Applied Biology, Shinshu University, - - 2014
Bacillus subtilis LytF plays a principal role in cell separation through its localisation at the septa and poles on the vegetative cell surface. In this study, we found that a mutation in a major lipoteichoic acid (LTA) synthase gene-ltaS-results in a considerable reduction in the σD-dependent transcription of lytF. Transcription ...
Wang Ling L Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy, Sichuan University, 610041, Chengdu, Sichuan, China, - - 2014
Increasing numbers of natural products have been found to possess anticancer effects. Nuclear factor erythroid-2-related factor-2 (Nrf2) is a master regulator of the antioxidative stress response, and our previous studies found that epigenetic modification of the Nrf2 gene appears to be a critical mechanism. Salvia miltiorrhiza, a Chinese herbal medicine ...
Peltonen Karita K Molecular Cancer Biology Program, University of - - 2014
Activation of the p53 pathway has been considered a therapeutic strategy to target cancers. We have previously identified several p53 activating small molecules in a cell-based screen. Two of the compounds activated p53 by causing DNA damage, but this modality was absent in the other four. We recently showed that ...
Fang Lei - - 2014
Cotton fiber, a highly elongated, thickened single cell of the seed epidermis, is a powerful cell wall research model. Fiber length, largely determined during the elongation stage, is a key property of fiber quality. Several studies using expressed sequence tags and microarray analysis have identified transcripts that accumulate preferentially during ...
Xu Xiao-Ling XL Department of Medical Oncology, Zhejiang Cancer Hospital, 38 Guangji Road, Hangzhou, - - 2014
The p53 protein is involved in many biological functions in cancer, such as cell cycle arrest, DNA repair, apoptosis, senescence, DNA metabolism, angiogenesis, and cellular differentiation. However, the association between p53 expression and clinicopathological findings or prognosis in esophageal squamous cell carcinoma (ESCC) is controversial. We designed a large-scale study ...
Chen Shu-Qin SQ *Department of Gynecology and Obstetrics, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; and †Department of Gynecology and Obstetrics, The People's Hospital of Guizhou Province, Guizhou, - - 2014
The current study was undertaken to investigate the predictive value of simultaneous enhancer of zeste homolog 2 (EZH2) and P53 expression in lesions of patients with cervical squamous cell carcinoma. Quantum dot double fluorescence staining was applied to detect EZH2 and P53 protein in biopsy tissue of 168 patients with ...
Ramdas Nisha M NM National Centre for Biological Sciences, TIFR, Bangalore, - - 2014
The nucleus is sculpted towards various morphologies during cellular differentiation and development. Alterations in nuclear shape often result in changes to chromatin organization and genome function. This is thought to be reflective of its role as a cellular mechanotransducer. Recent evidence has highlighted the importance of cytoskeletal organization in defining ...
Nakatsubo Takuya T Laboratory of Gene Regulation, Graduate School of Medicine and Pharmaceutical Sciences, University of - - 2014
In eukaryotes, the Mediator complex is an essential transcriptional cofactor of RNA polymerase II (Pol II). In humans, it contains up to 30 subunits and consists of four modules: head, middle, tail, and CDK/Cyclin. One of the subunits, MED15, is located in the tail module, and was initially identified as ...
Perriaud Laury L Institut Curie, Centre de Recherche, Bât. 110-112, Centre Universitaire, Orsay, F-91405, France Inserm, U612, Bât. 110-112, Centre Universitaire, Orsay, F-91405, - - 2014
G-quadruplex (G4) structures in intron 3 of the p53 pre-mRNA modulate intron 2 splicing, altering the balance between the fully spliced p53 transcript (FSp53, encoding full-length p53) and an incompletely spliced transcript retaining intron 2 (p53I2 encoding the N-terminally truncated Δ40p53 isoform). The nucleotides forming G4s overlap the polymorphism rs17878362 ...
DiMauro T T Department of Biochemistry and Molecular Pharmacology and NYU Cancer Institute, New York University School of Medicine, New York, NY, - - 2014
The Polycomb group protein Bmi-1 is an essential regulator of cellular senescence and is believed to function largely through the direct repression of the Ink4a/Arf locus. However, concurrent deletion of Ink4a/Arf does not fully rescue the defects detected in Bmi-1(-/-) mice, indicating that additional Bmi-1 targets remain to be identified. ...
Ichiyanagi Tomoko T Department of Immunology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Kita-ku, Okayama 700-8558, Japan Division of Epigenomics and Development, Medical Institute of Bioregulation, Kyushu University, Higashi-ku, Fukuoka 812-8582, - - 2014
HSP90, found in all kingdoms of life, is a major chaperone protein regulating many client proteins. We demonstrated that HSP90α, one of two paralogs duplicated in vertebrates, plays an important role in the biogenesis of fetal PIWI-interacting RNAs (piRNA), which act against the transposon activities, in mouse male germ cells. ...
Cadar Adrian G AG Division of Cardiovascular Medicine, Vanderbilt University School of Medicine, Nashville, TN, United States; Department of Molecular Physiology & Biophysics, Vanderbilt University School of Medicine, Nashville, TN, United - - 2014
Titin is the largest known protein and a critical determinant of myofibril elasticity and sarcomere structure in striated muscle. Accumulating evidence that mRNA transcripts are post-transcriptionally regulated by specific motifs located in the flanking untranslated regions (UTRs) led us to consider the role of titin 5'-UTR in regulating its translational ...
Wu Feili F College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, 22 Xinong Road, Yangling, Shaanxi 712100, - - 2014
Polychlorinated biphenyls (PCBs) are a group of environmental contaminants widely dispersed in aquatic system. Recent data have shown that Aroclor 1254 (a highly chlorinated PCB mixture) has the potential to induce oxidative stress. The antioxidant genes are usually up-regulated in response to the oxidative stress. However, the mechanisms underlying the ...
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