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Gonzalez J M JM a Department of Animal Sciences and Industry , Kansas State University , Manhattan , Kansas , - - 2014
The objective of the study was to examine the effect of Brahman genetics on collagen enzymatic crosslinking gene expression and meat tenderness. Steers were randomly selected to represent a high percentage Brahman genetics (n = 13), Half-Blood genetics (n = 13), Brangus genetics (n = 13), and a high percentage Angus genetics (n = 13). Muscle samples from ...
Hering Thomas M TM Spinal Cord and Brain Injury Research Center, Department of Anatomy & Neurobiology, University of Kentucky, Lexington, KY 40536; Department of Orthopaedic Surgery, Washington University School of Medicine, St Louis, MO - - 2014
This study describes a new mechanism controlling the production of alternatively-spliced isoforms of type II procollagen (Col2a1) in vivo. During chondrogenesis, precursor chondrocytes predominantly produce isoforms containing alternatively-spliced exon 2 (type IIA and IID) while Col2a1 mRNA devoid of exon 2 (type IIB) is the major isoform produced by differentiated ...
Ren Zongli Z Department of Cardiothoracic Surgery, Renmin Hospital of Wuhan University, #99 Ziyang Road, Wuhan, 430060, Hubei, - - 2014
Aortic dissections (AD) is characterized by some types of extracellular matrix (ECM) disorders, including fibrosis with collagen production. P54(nrb) /NonO is known to be involved in collagen formation. In this study, we examined whether AD is associated with abnormal P54(nrb) /NonO expression. Aortic specimens and serum were obtained from 10 ...
Liu Huanhuan H Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou, 310058, - - 2014
Tendons that connect muscles to bone are often the targets of sports injuries. The currently unsatisfactory state of tendon repair is largely attributable to the limited understanding of basic tendon biology. A number of tendon lineage-related transcription factors have recently been uncovered and provide clues for the better understanding of ...
Ren Zhanping Z Department of Cranio‑Maxillofacial Trauma Plastic Surgery, Stomatology Hospital of Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710004, P.R. - - 2014
CCN2 and CCN3 belong to the CCN family of proteins, which show a high level of structural similarity.Previous studies have shown that CCN2 mediates the ability of transforming growth factor (TGF)‑β to stimulate collagen synthesis, leading to keloid formation. CCN2 and CCN3 are opposing factors in regulating the promoter activity and ...
Su Shao-Ching SC Department of Orthodontics, Division of Applied Biosciences, Hiroshima University Graduate School of Biomedical and Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, - - 2014
Excessive mechanical stress is considered the major cause of temporomandibular joint osteoarthritis (TMJ-OA). High magnitude cyclic tensile strain (CTS) upregulates pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in chondrocytes, while selective cyclooxygenase (COX)-2 inhibition has been shown to be beneficial to cytokine-induced cartilage damage. However, the effect of selective COX-2 inhibitors ...
Hino Kenta K Institute of Biomedical & Health Sciences, University of - - 2014
The endoplasmic reticulum (ER) stress transducer, box B-binding factor 2 human homolog on chromosome 7 (BBF2H7), is a basic leucine zipper (bZIP) transmembrane transcription factor. This molecule is activated in response to ER stress during chondrogenesis. The activated BBF2H7 accelerates cartilage matrix protein secretion through the up-regulation of Sec23a, which ...
Weng Xinyu X Atherosclerosis Research Center, Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Pathophysiology, Nanjing Medical - - 2014
Collagen type I is the primary component of the extracellular matrix (ECM). Repression of collagen type I gene (COL1A2) transcription by the pro-inflammatory cytokine interferon gamma (IFN-γ) in vascular smooth muscle cells (VSMCs) is a key step during atherogenesis that leads to the destabilization of the atherosclerotic plaque. The epigenetic ...
Niu Li-Na LN Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Changle Xi Road 145, Xi'an, 710032, - - 2014
The present study investigated the effects of intrafibrillar-silicified collagen scaffolds (ISCS) on the osteogenic differentiation of human dental pulp stem cells (hDPSCs) in vitro and in vivo. The hDPSCs were co-cultured with ISCS or nonsilicified collagen scaffolds (NCS) in control medium (CM) or osteogenic differentiation medium (ODM). Cell cycle and ...
Porcellato Ilaria I Department of Veterinary Medicine, University of Perugia, 06126, Perugia, - - 2014
Feline eosinophilic dermatoses (FEDs) are common diseases of cats with an unknown pathogenesis. They are histologically characterized by an eosinophilic infiltration and often by the presence of flame figures (FFs) and/or areas of loss of tissue architecture, here termed necrotic foci (NF). It has been postulated that an alteration in ...
Zhang Min M Department of Respiratory and Intensive Care Medicine (ICU), Guangzhou Red Cross Hospital, Jinan University, Guangzhou, - - 2014
ABSTRACT Salvianolic acid B (Sal B), one of the major water-soluble compounds of Danshen (a popular Chinese herb), possesses many of the biological activities, such as antifibrogenic effect in liver and renal diseases. Transforming growth factor-β1 (TGF-β1) plays a central role in the development of pulmonary fibrosis by stimulating extracellular ...
Schmal Hagen H Department of Orthopedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg, Germany, - - 2014
The cell quality plays a decisive role in autologous chondrocyte implantation (ACI). Aim of the study was the analysis of in vivo interactions between synovial concentrations of cytokines and cell quality used for ACI. Knee lavage fluids of patients undergoing an ACI were examined for total protein content (TPC) and ...
Eskova Anastasia - - 2014
α2β1 integrin is one of the most important collagen-binding receptors and has been implicated in numerous widely spread thrombotic and immune diseases. α2β1 integrin is a potent tumour suppressor and its downregulation is associated with increased metastasis and poor prognosis in breast cancer. Currently, very little is known about the ...
Suzuki Nobuharu N Department of Biochemistry and Biophysics, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, - - 2014
Teneurin-4 (Ten-4), a transmembrane protein, is expressed in the nervous systems and the mesenchymal tissues, including the cartilage. However, the Ten-4 function in cartilage development remains unknown. Here, we showed that Ten-4 is a novel regulator of chondrogenesis. In situ hybridization analysis revealed that Ten-4 was highly expressed in the ...
Watanabe Teppei T Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan. Electronic address: - - 2014
We reported previously that hypoxia inducible factor (HIF)-1α was not degraded under normoxic condition with transforming growth factor (TGF)-β1 stimulation in human periodontal ligament (PDL) cells; however, the role of HIF-1α has been unclear. Recently, HIF-1α, working together with TGF-β1, has been reported to promote extracellular matrix accumulation under normoxic ...
Nijhuis Anke - - 2014
Intestinal fibrosis with stricture formation is a complication of Crohn's disease (CD) that may mandate surgical resection. Accurate biomarkers that reflect the relative contribution of fibrosis to an individual stricture are an unmet need in managing patients with CD. The microRNA (miR)-29 family has been implicated in cardiac, hepatic and ...
Chang Alex C Y AC Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, V5Z 1L3, - - 2014
Background: Valvuloseptal defects are the most common congenital heart defects. Notch signaling induced endothelial-to-mesenchymal-transition (EMT) in the atrioventricular canal (AVC) cushions at murine embryonic day (E)9.5 is a required step during early valve development. Insights to the transcriptional network that is activated in endocardial cells (EC) during EMT and how ...
Graham Julie R JR Inflammation and Remodeling Research Unit, Pfizer, Inc., Cambridge, MA - - 2014
Fibrosis is a chronic disease characterized by an excessive deposition of scar tissue in the affected organs. A central mediator of this process is transforming growth factor-β (TGF-β), which stimulates the production of extracellular matrix proteins such as collagens. MicroRNAs (miRNAs) have been implicated in both fibrosis as well as ...
Dzobo Kevin K International Centre for Genetic Engineering and Biotechnology (ICGEB), Cape Town Component, Wernher and Beit Building (South), UCT Campus, Anzio Road, Observatory 7925, Cape Town, South Africa; Division of Medical Biochemistry and MRC Oesophageal Cancer Unit, Faculty of Health Sciences, University of Cape Town, Cape Town, South - - 2014
Recent studies have emphasized the importance of the extracellular microenvironment in modulating cell growth, motility, and signaling. In this study we have evaluated the ability of a fibroblast derived-extracellular matrix (fd-ECM) to regulate type I collagen synthesis and degradation in fibroblasts. Fibroblasts were plated on plastic (control) or on fd-ECM ...
Watson Chris J CJ School of Medicine & Medical Science, UCD Conway Institute, University College Dublin, Ireland; Chronic Cardiovascular Disease Management Unit, St Vincent's Healthcare Group/St Michael's Hospital, Dublin, - - 2014
Abstract Understanding the impact of extracellular matrix sub-types and mechanical stretch on cardiac fibroblast activity is required to help unravel the pathophysiology of myocardial fibrotic diseases. Therefore, the purpose of this study was to investigate pro-fibrotic responses of primary human cardiac fibroblast cells exposed to different extracellular matrix components, including ...
Men Ruoting R Division of Digestive Diseases, West China Hospital, Sichuan University, Chengdu 610041, - - 2014
To evaluate plasma Nogo-B levels in liver cirrhotic patients and declare a novel molecular basis by which Nogo-B modulates hepatic stellate cells (HSCs) activation. Plasma Nogo-B levels from patients of liver cirrhosis were detected by enzyme-linked immuno-sorbent assay (ELISA). Rat primary HSCs were culture activated or stimulated with TGF-β. Activated ...
Zhang Jinbao J 1Chinese Materia Medica College, Tianjin University of Traditional Chinese Medicine, Tianjin, China 2Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China 3The Sixth Chinese Drugs Factory of Tianjin Zhongxin Pharmaceutical Co., Ltd., Tianjin, - - 2014
Suxiaojiuxin Pill (SX) is a famous Chinese formulated product, which has been used to treat coronary heart disease and angina pectoris in China. This study was carried out to investigate the effect and possible mechanism of SX on the stability of atherosclerotic plaque in ApoE-deficient mice. 6∼8 weeks old apoE-/- ...
Odorisio Teresa T Laboratory of Molecular and Cell Biology, Istituto Dermopatico dell'Immacolata (IDI)-IRCCS, Rome 00167, - - 2014
Recessive dystrophic epidermolysis bullosa (RDEB) is a genodermatosis characterised by fragile skin forming blisters that heal invariably with scars. It is due to mutations in the COL7A1 gene encoding type VII collagen, the major component of anchoring fibrils connecting the cutaneous basement membrane to the dermis. Identical COL7A1 mutations often ...
Ovchinnikova Olga A OA Department of Medicine, Center for Molecular Medicine, University Hospital, Stockholm, Sweden; Almazov Federal Heart, Blood and Endocrinology Centre, St. Petersburg, - - 2014
Acute clinical complications of atherosclerosis such as myocardial infarction (MI) and ischemic stroke are usually caused by thrombus formation on the ruptured plaque surface. Collagen, the main structural protein of the fibrous cap, provides mechanical strength to the atherosclerotic plaque. The integrity of the fibrous cap depends on collagen fiber ...
Ma Qing Q Department of Gerontology, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, - - 2014
To investigate the effects of Herbal Compound 861 (Cpd 861) on collagen synthesis and degradation in rat mesangial cells exposed to high glucose. The third to fifth passage of rat mesangial cells were exposed to high glucose and Cpd 861 at a concentration of 0.25-4.00 g/L for 24, 48 and ...
Pei Xinhong X Department of Orthopaedics, Children's Hospital of Fudan University, 399 Wanyuan Road, Minhang District, Shanghai, 201102, China, - - 2014
Importance of actin organization in control of chondrocyte phenotype is well established, but little is known about the role of transforming growth factor-β1 (TFGβ1) in regulating of ROCK I signal pathway. Here, we investigated the role of the TGFβ1, a well-studied member of the TGF-β superfamily, in chondrogenesis. Newborn Rats ...
Yang Lingli L Department of Dermatology, Osaka University Graduate School of Medicine, Osaka, - - 2014
Histamine is thought to play a critical role in the synthesis of extracellular matrix in skin and may be involved in tissue remodeling of allergic diseases. Recent studies revealed that periostin, a matricelluar protein, contributed to tissue remodeling; however, a link between periostin and histamine remains unproven. We investigated whether ...
Huisman Elise S ES Department of Physical Therapy, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada; Centre for Hip Health and Mobility, Vancouver, BC, - - 2014
The aim of this study was: (i) to analyze the morphology and expression of extracellular matrix genes in six different regions of the Achilles tendon complex of intact normal rabbits; and (ii) to assess the effect of ovariohysterectomy (OVH) on the regional expression of these genes. Female New Zealand White ...
Saitta Biagio B United States ; - - 2014
Skeletal Dysplasias (SDs) are caused by abnormal chondrogenesis during cartilage growth plate differentiation. To study early stages of aberrant cartilage formation in vitro, we generated the first induced Pluripotent Stem Cells (iPSCs) from fibroblasts of an SD patient with a lethal form of metatropic dysplasia, caused by a dominant mutation ...
Bungeroth May M Department of Neurology, University of Kiel, Kiel, - - 2014
Pathologic aggregation of α-synuclein is a central process in the pathogenesis of Parkinson's disease. The α-synuclein gene (SNCA) encodes at least 4 different α-synuclein isoforms through alternative splicing (SNCA140, SNCA126, SNCA112, SNCA98). Differential expression of α-synuclein isoforms has been shown in Lewy body diseases. In contrast to the canonical α-synuclein ...
Léotoing Laurent L INRA, UMR 1019, UNH, CRNH Auvergne, Clermont-Ferrand, France; Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, Clermont-Ferrand, - - 2014
Flavonoids represent a group of polyphenolic compounds commonly found in daily nutrition with proven health benefits. Among this group, the flavonol fisetin has been previously shown to protect bone by repressing osteoclast differentiation. In the present study, we investigated the role of fisetin in regulating osteoblasts physiology. In vivo mice ...
Assis de Brito Thatiana L TL Department of Histology and Embryology, State University of Rio de Janeiro, Rio de Janeiro, - - 2014
β-Adrenoceptors modulate acute wound healing; however, few studies have shown the effects of β-adrenoceptor blockade on chronic wounds. Therefore, this study the investigated effect of β1-/β2-adrenoceptor blockade in wound healing of pressure ulcers. Male mice were daily treated with propranolol (β1-/β2-adrenoceptor antagonist) until euthanasia. One day after beginning of treatment, ...
Enochson Lars L Department of Clinical Chemistry and Transfusion Medicine, The Sahlgrenska Academy at Gothenburg University, Gothenburg, Sweden. Electronic address: - - 2014
GDF5 is important for joint formation and associated with osteoarthritis. Its role for the homeostasis of cartilage extracellular matrix is, however, unknown. The canonical Wnt signaling pathway is also implemented in osteoarthritis and activation of the pathway has detrimental effects on the cartilage extracellular matrix. The objective of this study ...
Honzawa Yusuke Y Department of Gastroenterology and Hepatology, Graduate School of Medicine, Kyoto University, , Kyoto, Kyoto, - - 2014
Intestinal fibrosis is a clinically important issue in Crohn's disease (CD). Heat shock protein (HSP) 47 is a collagen-specific molecular chaperone involved in fibrotic diseases. The molecular mechanisms of HSP47 induction in intestinal fibrosis related to CD, however, remain unclear. Here we investigated the role of interleukin (IL)-17A-induced HSP47 expression ...
Tamaki Zenshiro Z Department of Dermatology, University of Tokyo Graduate School of Medicine, Tokyo, - - 2014
Rapamycin has been shown to exert an anti-fibrotic effect on skin fibrosis in a certain subset of patients with systemic sclerosis (SSc) and in bleomycin-treated animal models. To investigate the mechanism responsible for the anti-fibrotic effect of rapamycin especially by focusing on human α2(I) collagen (COL1A2) and matrix metalloproteinase 1 ...
Li Zhengwei Z Institution: Department of Cardiology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, PR - - 2014
Farnesyl pyrophosphate synthase (FPPS)-catalysed isoprenoid intermediates are important for the activation of RhoA in Ang II-induced cardiac fibrosis. This study was designed to investigate the specific role of FPPS in the development of cardiac fibrosis. We demonstrated that FPPS expression was elevated in both in vivo and in vitro models ...
Nyp Michael F - - 2014
Myofibroblasts are the critical effector cells in the pathogenesis of pulmonary fibrosis which carries a high degree of morbidity and mortality. We have previously identified Type II TGFbeta receptor interacting protein 1 (TRIP-1), through proteomic analysis, as a key regulator of collagen contraction in primary human lung fibroblasts--a functional characteristic ...
Wang Yi-Wen YW 1] Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei City, Taiwan, Republic of China [2] Burn Center, Tri-Service General Hospital, Taipei City, Taiwan, Republic of - - 2014
Hypertrophic scarring is related to persistent activation of transforming growth factor-β (TGF-β)/Smad signaling. In the TGF-β/Smad signaling cascade, the TGF-β type I receptor (TGFBRI) phosphorylates Smad proteins to induce fibroblast proliferation and extracellular matrix deposition. In this study, we inhibited TGFBRI gene expression via TGFBRI small interfering RNA (siRNA) to ...
Tauzin H H UMR 1098, SFR FED 4234, 25000, Besançon, France, - - 2014
Prevalence of leg ulcer in general population is important and new efficient treatments are now needed, especially for chronic leg ulcers. Human amniotic membrane (HAM) can be used as an alternative treatment for recalcitrant leg ulcers. The aim of this study is to investigate the effects of a HAM extract ...
Gruber James V JV Lonza Consumer Care, 70 Tyler Place, South Plainfield, NJ 07080. Electronic address: - - 2014
Pomiferin is a unique, prenylated isoflavonoid that can be isolated and purified from the fruits of Maclura pomifera (Osage Orange). The molecule typically is isolated with a small amount of a molecule called Osajin which is structurally similar to Pomiferin but lacks an aromatic hydroxyl group. As a consequence, Osajin ...
Yoshizawa Sayuri S Department of Oral Biology, University of Pittsburgh, Pittsburgh, PA, USA.; The Center for Craniofacial Regeneration, University of Pittsburgh, Pittsburgh, PA, USA.; The McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, - - 2014
Magnesium (Mg) alloys are being investigated for load bearing bone fixation devices due to their initial mechanical strength, modulus similar to native bone, biocompatibility and ability to degrade in vivo. Previous studies have found Mg alloys to support bone regeneration in vivo, but the mechanisms have not been investigated in ...
Mathieu Marc M Inserm, - - 2014
Mesenchymal stem cells (MSCs) are considered for cartilage engineering given their ability to differentiate into chondrocytes. Chondrogenic differentiation of MSCs is currently triggered by micromass culture in the presence of a member of the TGF-β superfamily. However, the main constituents of the cartilaginous matrix, aggrecan and type II collagen, are ...
Wang Wei-Li WL Institute of Cellular and System Medicine, National Health Research Institutes, Miaoli 350, - - 2014
Human telomerase reverse transcriptase (hTERT) and oncogene c-Myc have been shown to regulate cell proliferation. Our previous studies demonstrated that fibrillar collagen mediates vascular smooth muscle cell (SMC) cycle progression and proliferation in response to platelet-derived growth factor (PDGF)-BB and interleukin (IL)-1β. However, whether hTERT and c-Myc are involved in ...
Yuan Li-Jen - - 2014
Although the individual effects of hyperbaric oxygen (HBO) and low-intensity pulsed ultrasound (LIPUS) on osteoarthritic (OA) chondrocytes have been reported, the effects of HBO combined with LIPUS treatment are unknown. OA chondrocytes were obtained from patients undergoing knee replacement surgery. RNA was isolated for real-time polymerase chain reaction (PCR) analysis ...
Conejero-Goldberg C C Litwin Zucker Center for the Study of Alzheimer's Disease, Feinstein Institute, Hofstra North Shore-LIJ School of Medicine, Manhasset, NY, - - 2014
The common APOE2 gene variant is neuroprotective against Alzheimer's disease (AD) and reduces risk by nearly 50%. However, the mechanisms by which APOE2 confers neuroprotection are largely unknown. Here we showed that ApoE protein abundance in human postmortem cortex follows an isoform-dependent pattern (E2>E3>E4). We also identified a unique downstream ...
Luo Na N The Brody School of Medicine at East Carolina University, Greenville, North Carolina and School of Medicine, Nankai, University, Tianjin, P. - - 2014
Objective: Cell-matrix interactions promote cartilage homeostasis. In our previous work, SMAD1, the transcriptional modulator of the canonical BMP7 pathway, was found to interact with the cytoplasmic domain of CD44 -- the principal hyaluronan receptor on chondrocytes. To elucidate the physiological function of CD44-SMAD1 interaction, as well as the role of ...
Wang Yu Peng YP Health and Heart Center, the Beijing Friendship Hospital affiliated the Capital Medical University, Beijing 100050, - - 2014
Relaxin is known to inhibit cardiac fibrosis. However, it is unclear whether relaxin could regulate the effects of Phorbol 12-myristate 13-acetate (PMA, PKC activator) on cardiac fibrosis. So the influence of relaxin on the cell proliferation and collagen expression induced by PMA in cultured cardiac fibroblasts was studied. It showed ...
Rao Velidi H VH Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, 68178, - - 2014
Mechanisms underlying the rupture of atherosclerotic plaque, a crucial factor in the development of myocardial infarction and stroke, are not well defined. Here, we examined the role of epidermal growth factor (EGF)-mediated matrix metalloproteinases (MMP) on the stability of interstitial collagens in vascular smooth muscle cells (VSMCs) isolated from carotid ...
Ansboro Sharon S Regenerative Medicine Institute (REMEDI), National University of Ireland - - 2014
Human mesenchymal stem cells (hMSCs) have been identified as a viable cell source for cartilage tissue engineering. However, to undergo chondrogenic differentiation hMSCs require growth factors, in particular members of the transforming growth factor beta (TGF-β) family. While in vitro differentiation is feasible through continuous supplementation of TGF-β3, mechanisms to ...
Wu Wen-Tzu WT School of Nutrition, Chung Shan Medical University, No. 110, Sec. 1 Jianguo N. Rd., Taichung City 402, - - 2014
Mice were fed low-fibre, or that supplemented with soluble fibre (konjac glucomannan, KGM; inulin), or insoluble fibre (cellulose) to determine how these three fibres modulated the acute colonic responses to an azoxymethane (AOM) treatment. Results indicated that KGM and inulin exerted greater anti-genotoxic effects compared to cellulose and up-regulated the ...
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