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Marfil V V Unidad de Hepatología Experimental, CIBERehd, Instituto de Investigación Sanitaria La Fe, Avda. Fernando Abril Martorell 106, Valencia, - - 2014
c-Myc transcription factor is a key protein involved in cellular growth, proliferation and metabolism. c-Myc is one of the most frequently activated oncogenes, highlighting the need to identify intracellular molecules that interact directly with c-Myc to suppress its function. Here we show that Hhex is able to interact with the ...
Trucco Lucas Daniel LD Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, - - 2014
KLF6 is a member of the Krüppel-like factor family of transcription factors, with diverse roles in the regulation of cell physiology, including proliferation, signal transduction, and apoptosis. Mutations or down-regulation of KLF6 have been described in several human cancers. In this work, we found that KLF6-knockdown resulted in the formation ...
Tecleab Awet A Memorial Sloan Kettering Cancer Center, United - - 2014
Ral GTPases are critical effectors of Ras, yet the molecular mechanism by which they induce malignant transformation is not well understood. In this manuscript, we found the expression of K-Ras, RalB, and sometimes RalA, but not AKT1/2 and c-Raf, to be required for maintaining low levels of p53 in human ...
Chai Jinyu J MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, - - 2014
Plant senescence is a highly regulated process that can be induced by a range of factors. The nonexpressor of pathogenesis-related genes 1 (npr1) mutant is defective in the salicylic acid (SA) signalling pathway, displaying delayed yellowing during developmental senescence. However, the regulating mechanism of NPR1 on exogenous SA-induced senescence in ...
Shi Guang G East China Normal University, - - 2014
Regulation of rDNA transcription is central to cell growth and proliferation. PHF2 and PHF8 are enriched in the nucleolus and belong to a subfamily of histone demethylases that also possess a PHD domain-dependent binding activity for di/tri-methylated histone 3 lysine 4 (H3K4me2/3). In this study, we show that, unlike PHF8 ...
Yan Chunxia C State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, - - 2014
The Tudor-SN protein (TSN) is universally expressed and highly conserved in eukaryotes. In Arabidopsis, TSN is reportedly involved in stress adaptation, but the mechanism involved in this adaptation is not understood. Here, we provide evidence that TSN regulates the mRNA levels of GA20ox3, a key enzyme for gibberellin (GA) biosynthesis. ...
Zhu Tianqing T Swedish University of Agricultural Sciences, Department of Plant Biology, Uppsala BioCenter, Linnean Center of Plant Biology in Uppsala, PO-Box 7080, SE-75007 Uppsala, Sweden - - 2014
Proper embryo development is crucial as that is when the primary body axes are established. In Arabidopsis, AtWOX8 and AtWOX9, members of the WUSCHEL-RELATED HOMEOBOX (WOX) gene family, are critical for embryo development. In Norway spruce, PaWOX8/9, which is expressed in embryos, is the homologue of AtWOX8 and AtWOX9. In ...
Zhao Tong T State Key Laboratory of Medicinal Chemical Biology, Collaborative Innovation Center for Biotherapy, 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics and College of Life Sciences, Nankai University, Tianjin, 300071, - - 2014
Non-coding RNAs (ncRNAs), such as microRNAs and large intergenic non-coding RNAs, have been shown to play essential roles in regulating pluripotency. Yet, it is not clear the role of natural antisense transcripts (NATs), also belonging to ncRNAs, in embryonic stem cells. However, the role of NATs in embryonic stem cells ...
Moon Heegyum H School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, - - 2014
The product of proto-oncogene Ron is a human receptor for the macrophage-stimulating protein (MSP). Upon activation, Ron is able to induce cell dissociation, migration and matrix invasion. Exon 11 skipping of Ron pre-mRNA produces Ron△165 protein that is constitutively active even in the absence of its ligand. Here we show ...
Patwardhan Gauri A GA Department of Basic Pharmaceutical Sciences, University of Louisiana at Monroe, Monroe, LA, - - 2014
Mutants of tumor suppressor p53 not only lose the activity in genome stabilizing and in tumor suppression, but also exhibit oncogenic function in cancer cells. Most efforts in restoring p53 biological activity focus on either altering mutant-protein conformation or introducing an exogenous p53 gene into cells to eliminate p53-mutant cancer ...
Akita Hirofumi H Department of Surgery, Osaka University, Graduate School of - - 2014
Activation of c-MYC is an oncogenic hallmark of many cancers including liver cancer, and is associated with a variety of adverse prognostic characteristics. Despite a causative role during malignant transformation and progression in hepatocarcinogenesis, consequences of c-MYC activation for the biology of hepatic cancer stem cells (CSCs) are undefined. Here, ...
Das Kausik K Research and Development Division, Streck Inc., 7002 S 109 Street, Omaha, NE, 68128, - - 2014
Messenger RNA (mRNA) expression levels in blood cells are important in disease diagnosis, prognosis and biomarker discovery research. Accurate measurements of intracellular mRNA levels in blood cells depend upon several pre-analytical factors, including delays in RNA extraction from blood after phlebotomy. Dramatic changes in mRNA expression levels caused by delays ...
Camozzi Daria D CNR Institute for Molecular Genetics; Unit of Bologna and SC Laboratory of Muscoskeletal Cell Biology; Rizzoli Orthopedic Institute; Bologna, - - 2014
Interconnected functional strategies govern chromatin dynamics in eukaryotic cells. In this context, A and B type lamins, the nuclear intermediate filaments, act on diverse platforms involved in tissue homeostasis. On the nuclear side, lamins elicit large scale or fine chromatin conformational changes, affect DNA damage response factors and transcription factor ...
Kizil Caghan C DFG-Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Fetscherstrasse 105, Dresden 01307, - - 2014
Canonical β-catenin-dependent Wnt signal transduction is important for several biological phenomena, such as cell fate determination, cell proliferation, stem cell maintenance and anterior-posterior axis formation. The hallmark of canonical Wnt signaling is the translocation of β-catenin into the nucleus where it activates gene transcription. However, the mechanisms regulating β-catenin nuclear ...
Shin Jun-Hye JH School of Biological Sciences, University of Missouri - Kansas City, Kansas City, Missouri, United States of - - 2014
The exosome complex functions in RNA metabolism and transcriptional gene silencing. Here, we report that mutations of two Arabidopsis genes encoding nuclear exosome components AtRRP6L1 and AtRRP6L2, cause de-repression of the main flowering repressor FLOWERING LOCUS C (FLC) and thus delay flowering in early-flowering Arabidopsis ecotypes. AtRRP6L mutations affect the ...
Zhang Bingqing B Department of Biology, Indiana University, Bloomington, Indiana, United States of - - 2014
Apoptotic cell death is an important response to genotoxic stress that prevents oncogenesis. It is known that tissues can differ in their apoptotic response, but molecular mechanisms are little understood. Here, we show that Drosophila polyploid endocycling cells (G/S cycle) repress the apoptotic response to DNA damage through at least ...
Cao Zipeng Z Department of Occupational and Environmental Health and Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an, China. Nelson Institute of Environmental Medicine, New York University School of Medicine, Tuxedo, NY, - - 2014
The inhibitor of apoptosis protein XIAP (X-linked inhibitor of apoptosis protein) is a well-documented protein that is located in cytoplasm acting as a potent regulator of cell apoptosis. Here, we showed that expressing XIAP with RING (Really Interesting New Gene) domain deletion (XIAP△RING) in cancer cells promoted cancer cell anchorage-independent ...
Maniam S S Laboratory of Cancer Biology, Department of Oncology, Medical Science Division, University of Oxford, Oxford, - - 2014
Metabolic reprogramming is a hallmark of cancer cells. Strap (stress-responsive activator of p300) is a novel TPR motif OB-fold protein that contributes to p53 transcriptional activation. We show here that, in addition to its established transcriptional role, Strap is localised at mitochondria where one of its key interaction partners is ...
Ayroldi E E Department Medicine, Section of Pharmacology, University of Perugia Medical School, Perugia, - - 2014
The transcription factor p53 regulates the expression of genes crucial for biological processes such as cell proliferation, metabolism, cell repair, senescence and apoptosis. Activation of p53 also suppresses neoplastic transformations, thereby inhibiting the growth of mutated and/or damaged cells. p53-binding proteins, such as mouse double minute 2 homolog (MDM2), inhibit ...
Kaneko Syuzo S Howard Hughes Medical Institute, Department of Biochemistry and Molecular Pharmacology, New York University Langone School of Medicine, New York 10016, - - 2014
Polycomb-repressive complex 2 (PRC2) facilitates the maintenance and inheritance of chromatin domains repressive to transcription through catalysis of methylation of histone H3 at Lys27 (H3K27me2/3). However, through its EZH2 subunit, PRC2 also binds to nascent transcripts from active genes that are devoid of H3K27me2/3 in embryonic stem cells. Here, biochemical ...
Parry Geraint G Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK - - 2014
The nuclear pore complex (NPC) is a multisubunit protein conglomerate that facilitates movement of RNA and protein between the nucleus and cytoplasm. Relatively little is known regarding the influence of the Arabidopsis NPC on growth and development. Seedling development, flowering time, nuclear morphology, mRNA accumulation, and gene expression changes in ...
Chittaranjan Suganthi S Canada's Michael Smith Genome Sciences Centre, BC Cancer Agency, Vancouver, BC, - - 2014
The majority of oligodendrogliomas (ODGs) exhibit combined losses of chromosomes 1p and 19q and mutations of isocitrate dehydrogenase (IDH1-R132H or IDH2-R172K). Approximately 70% of ODGs with 1p19q co-deletions harbor somatic mutations in the Capicua Transcriptional Repressor (CIC) gene on chromosome 19q13.2. Here we show that endogenous long (CIC-L) and short ...
Chao Chuck C-K CC Department of Biochemistry and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan, Republic of China; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan, Republic of China. Electronic address: - - 2014
The tumor suppressor p53 plays various functional roles in the cell by regulating multiple regulatory signals that ensure adequate temporal and spatial response to cellular stress. p53 is usually kept inactive due to ubiquitination by a number of E3 ubiquitin ligases that target p53 for proteasomal degradation. The ubiquitously expressed ...
Shimizu Rina R Department of Biotechnology, Kyoto Institute of Technology, Kyoto 606-8585, - - 2014
Mitochondrial frataxin functions in iron homeostasis, biogenesis of iron-sulfur clusters, protection from oxidative stress and apoptosis, and as a tumor suppressor protein. We examined regulation of the expression of the human frataxin by p53. Pifithrin-α, an inhibitor of p53 function, and knockdown of p53 decreased the level of frataxin mRNA ...
Liu Ju J Department of Radiation Oncology, Rutgers Cancer Institute of New Jersey, Rutgers, State University of New Jersey, New Brunswick, NJ, - - 2014
Tumor suppressor p53 has a key role in maintaining genomic stability and preventing tumorigenesis through its regulation of cellular stress responses, including apoptosis, cell cycle arrest and senescence. To ensure its proper levels and functions in cells, p53 is tightly regulated mainly through post-translational modifications, such as ubiquitination. Here, we ...
Gao Kang K Hebei Key Laboratory of Molecular and Cellular Biology, Hebei 050024, P.R. China Key Laboratory of Molecular and Cellular Biology of Ministry of Education, College of Life Science, Hebei Normal University, Hebei 050024, P.R. China Hebei Collaboration Innovation Center for Cell Signaling, Shijiazhuang, Hebei 050024, P.R. - - 2014
The heat stress response provides an important adaptation, enabling plants to survive challenging environmental conditions. Our previous work demonstrated that Arabidopsis thaliana Phosphoinositide-Specific Phospholipase C Isoform 9 (AtPLC9) plays an important role in thermotolerance. During prolonged heat treatment, mutants of AtPLC3 showed decreased heat resistance. We observed no obvious phenotypic ...
Wirth Matthias M Medizinische Klinik, Technische Universität München, München 81675, - - 2014
The c-MYC (MYC afterward) oncogene is well known for driving numerous oncogenic programs. However, MYC can also induce apoptosis and this function of MYC warrants further clarification. We report here that a clinically relevant proteasome inhibitor significantly increases MYC protein levels and that endogenous MYC is necessary for the induction ...
Mahe Etienne E Department of Pathology & Laboratory Medicine, University of Calgary/Calgary Laboratory Services, Calgary, AB, - - 2014
Mantle cell lymphoma (MCL) is an aggressive disease with poor overall survival, attributable in part to frequent defects of the DNA repair genes. In such malignancies, additional inhibition of the ubiquitous DNA damage repair protein, poly-ADP ribose polymerase-1 (PARP1) has shown enhanced cytotoxicity (so-called synthetic lethality). We studied PARP1 expression ...
Kim Jongdoo J Division of Radiation Cancer Biology, Korea Institute of Radiological & Medical Sciences, Seoul, Korea Department of Radiological Cancer Medicine, University of Science and Technology, Seoul, - - 2014
How the p53 transcription factor/tumor suppressor inhibits cell invasion is poorly understood. We demonstrate that this function of p53 requires its direct interaction with p21(WAF1), a transcriptional target of p53, and that both p21 and p53 bind to Slug, which promotes cell invasion. Functional studies reveal that p21 and p53 ...
Liu Lei L Osteopathy Ward, The First Affiliated Hospital, Research Center for Regenerative Medicine, Guangxi Medical University, Nanning, 530021, - - 2014
Antioxidant may provide anti-arthritic effect that contributes to resolution of inflammation. Gallic acid (GA) and its derivatives were reported to be effective in treatment of arthritis. But GA-suppressed cell proliferation may compromise its effect on chondro-protection. In this study, we synthesized sulfonamido-based gallate-JEZTC and investigated its effect on rabbit articular ...
Tsou Pei-Suen - - 2014
IntroductionSystemic sclerosis (SSc) is a connective tissue disease characterized by fibrosis of the skin and organs. Increase in oxidative stress and platelet-derived growth factor receptor (PDGFR) activation promote collagen I (Col I) production, leading to fibrosis in SSc. Lipoic acid (LA) and its active metabolite dihydrolipoic acid (DHLA) are naturally ...
Ruthard Johannes J Institute for Clinical Chemistry, University of Cologne, - - 2014
Abstract Purpose/Aim of the study: We investigated whether COMP may modify cartilage metabolism and play a role as an endogenous disease aggravating factor in OA. Materials and Methods: Full-length and momomeric COMP was recombinantly expressed in human embryonic kidney cells and purified it via affinity chromatography. Purified COMP was used ...
Liu Nian N National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, - - 2014
microRNAs (miRNAs) are 20-24 nucleotide non-coding small RNAs that play important roles in plant development. The stages of cotton fiber development include initiation, elongation, secondary wall thickening (SWT) and maturation. We constructed seven fiber RNA libraries representing the initiation, elongation and SWT stages. A total of 47 conserved miRNA families ...
Lambers Christopher C Division of Respiratory Medicine, Department of Internal Medicine II, Medical University of Vienna, A-1090 Vienna, Austria. Electronic address: - - 2014
Long acting β2-agonists (LABA) have been reported to modify the extracellular matrix (ECM) composition in the airway wall. Based on our earlier studies we here investigated the mechanism underlying the control of ECM modification by LABA in primary human airway smooth muscle cells. Cells were treated with formoterol or salmeterol ...
Maneix Laure L Normandy University, Caen, France; UNICAEN, Laboratoire Microenvironnement Cellulaire et Pathologies (MILPAT), EA4652, 14032, Caen, - - 2014
The existence of a link between estrogen deprivation and osteoarthritis (OA) in postmenopausal women suggests that 17β-estradiol (17β-E2) may be a modulator of cartilage homeostasis. Here, we demonstrate that 17β-E2 stimulates, via its receptor human estrogen receptor α 66 (hERα66), type II collagen expression in differentiated and dedifferentiated (reflecting the ...
Hennemeier Isabell I Julius-Bernstein-Institut für Physiologie, Martin-Luther-Universität Halle-Wittenberg, Magdeburger Straße 6, 06112 Halle (Saale), - - 2014
Ochratoxin A (OTA) is an ubiquitous mycotoxin suspected to cause fibrotic kidney diseases. The involvement of mircoRNAs in these processes is unknown. Here, we investigated the role of the anti-fibrotic miR-29b in OTA-induced alterations of cellular collagen homeostasis. OTA exposure of human embryonic kidney cells (HEK293) cells led to an ...
Asai Nobuyuki N Division of Neurogenetics, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya, Japan; Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine, Nagoya, - - 2014
Endochondral ossification is an essential step for skeletal development, which requires chondrocyte differentiation in growth cartilage. The low-density lipoprotein receptor-related protein 4 (LRP4), a member of LDLR family, is an inhibitor for Wnt signaling, but its roles in chondrocyte differentiation remain to be investigated. Here we found by lasercapture microdissection ...
Kim Kyungbin K Department of Pathology, Pusan National University Yangsan Hospital, Yangsan, - - 2014
Epithelial-mesenchymal transition (EMT) is associated with tumor hypoxia. EMT is regulated, in part, by the action of TWIST, which inhibits of E-cadherin expression and may interfere with the p53 tumor-suppressor pathway. We examined the expression of TWIST, E-cadherin, hypoxia-inducible factor 1α (HIF1α), and p53 by immunohistochemistry in 123 cases of ...
Bi Yan Y Mayo - - 2014
Background/Aims: Hepatic stellate cells (HSCs) generate matrix which in turn may also regulate HSCs function during liver fibrosis. We hypothesized that HSCs may endocytose matrix proteins to sense and respond to changes in microenvironment. Methods: Primary human HSCs or mouse embryonic fibroblasts (MEFs; wildtype, c-abl(-/-) or YSF(-/-)) were incubated with ...
Sato Iwao I Department of Anatomy, School of Life Dentistry at Tokyo, Tokyo, Japan. Electronic address: - - 2014
The masseter muscle (MM) is a complex tendinous laminar structure during development; however, the stage of the laminar structure formation is unknown. Tenomodulin (TeM) is a useful marker of tendons and has an anti-angiogenic cysteine-rich C-terminal domain. Therefore, we analyzed mRNA of TeM and angiogenesis markers (CD31 and vascular endothelial ...
Li Wei-Jun WJ Department of Orthopedic Surgery, The Second Affiliated Hospital of Medical College, Zhejiang University, Jie Fang Road 88#, 310009 Hangzhou, People's Republic of - - 2014
Dehydroepiandrosterone (DHEA) and its ester form, DHEA-S, are the most abundant steroids in human plasma. Our previous studies showed that DHEA protects against osteoarthritis (OA). The aim of this paper was to explore the possible mechanisms that underlie DHEA-mediated protection against OA. We tested the expression of β-catenin, it was ...
Chen Xi X Department of Stomatology, First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, China. Electronic address: - - 2014
To investigate the expression changes of collagen types I and III (Coll I and III) and matrix metalloproteinase-1 (MMP-1) in the periodontal ligament on the tension side of the distracted tooth using periodontal ligament distraction osteogenesis in beagle dogs. Twenty Beagle dogs were randomly divided into five groups of four ...
- - 2014
TARBP2 binding to sRSEs within metastasis suppressor transcripts promotes their decay.
Salem Omar - - 2014
We previously showed that type X collagen, a marker of late stage chondrocyte hypertrophy (associated with endochondral ossification), is constitutively expressed by mesenchymal stem cells (MSCs) from osteoarthritis patients and this may be related to Naproxen (Npx), a nonsteroidal anti-inflammatory drug used for therapy. Hedgehog (HH) signaling plays an important ...
- - 2014
Mutant IDH prevents hepatocyte differentiation and promotes tumorigenesis via HNF-4α inhibition.
Amano Katsuhiko K Harvard School of Dental Medicine, United - - 2014
Indian hedgehog (Ihh) is essential for chondrocyte differentiation and endochondral ossification and acts with PTHrP in a negative-feedback loop to regulate early chondrocyte differentiation and entry to hypertrophic differentiation. Independent of this function, we and others recently reported independent Ihh functions to promote chondrocyte hypertrophy and matrix mineralization in vivo ...
Duarte M S MS Department of Animal Science, Universidade Federal de Viçosa, MG, Brazil - - 2014
Twenty-four pregnant Nellore cows were randomly assigned into 2 feeding level groups (Control - CTL, fed 1.0 times the maintenance requirement, n = 12; and Overnourished - ON fed at 1.5 times the maintenance requirement, n = 12) to evaluate effects of maternal overnutrition on fetal skeletal muscle development. Cows ...
Wu Ting - - 2014
Systemic sclerosis (SSc) is a connective tissue fibrotic disease for which there is no effective treatment. Traditional Chinese Medicine (TCM), such as the Yiqihuoxue formula used in Shanghai TCM-integrated Hospital, has shown the efficacy of anti-fibrosis in clinical applications. This study was aiming to dissect the anti-fibrotic mechanism of Yiqihuoxue ...
Gonzalez J M JM a Department of Animal Sciences and Industry , Kansas State University , Manhattan , Kansas , - - 2014
The objective of the study was to examine the effect of Brahman genetics on collagen enzymatic crosslinking gene expression and meat tenderness. Steers were randomly selected to represent a high percentage Brahman genetics (n = 13), Half-Blood genetics (n = 13), Brangus genetics (n = 13), and a high percentage Angus genetics (n = 13). Muscle samples from ...
Cucchiarini M M Center of Experimental Orthopaedics, Saarland University Medical Center, Homburg/Saar, - - 2014
Direct therapeutic gene transfer is a promising tool to treat articular cartilage defects. Here, we tested the ability of an recombinant adeno-associated virus (rAAV) insulin-like growth factor I (IGF-I) vector to improve the early repair of cartilage lesions in vivo. The vector was administered for 3 weeks in osteochondral defects ...
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