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Salem Omar - - 2014
We previously showed that type X collagen, a marker of late stage chondrocyte hypertrophy (associated with endochondral ossification), is constitutively expressed by mesenchymal stem cells (MSCs) from osteoarthritis patients and this may be related to Naproxen (Npx), a nonsteroidal anti-inflammatory drug used for therapy. Hedgehog (HH) signaling plays an important ...
Hermida N N Catholic University of Louvain (UCL), Pole of Pharmacology and Therapeutics, Brussels, - - 2014
Cardiac fibrosis is an integral part of myocardial remodelling in response to stress. Mice with cardiac myocyte-specific overexpression of beta3-adrenergic receptor (β3-AR) are protected not only from the development of hypertrophy but also cardiac fibrosis. As overexpression was restricted to cardiac myocytes (CM), we postulated that the protection involved a ...
Amano Katsuhiko K Harvard School of Dental Medicine, United - - 2014
Indian hedgehog (Ihh) is essential for chondrocyte differentiation and endochondral ossification and acts with PTHrP in a negative-feedback loop to regulate early chondrocyte differentiation and entry to hypertrophic differentiation. Independent of this function, we and others recently reported independent Ihh functions to promote chondrocyte hypertrophy and matrix mineralization in vivo ...
Duarte M S MS Department of Animal Science, Universidade Federal de Viçosa, MG, Brazil - - 2014
Twenty-four pregnant Nellore cows were randomly assigned into 2 feeding level groups (Control - CTL, fed 1.0 times the maintenance requirement, n = 12; and Overnourished - ON fed at 1.5 times the maintenance requirement, n = 12) to evaluate effects of maternal overnutrition on fetal skeletal muscle development. Cows ...
Wu Ting - - 2014
Systemic sclerosis (SSc) is a connective tissue fibrotic disease for which there is no effective treatment. Traditional Chinese Medicine (TCM), such as the Yiqihuoxue formula used in Shanghai TCM-integrated Hospital, has shown the efficacy of anti-fibrosis in clinical applications. This study was aiming to dissect the anti-fibrotic mechanism of Yiqihuoxue ...
Gonzalez J M JM a Department of Animal Sciences and Industry , Kansas State University , Manhattan , Kansas , - - 2014
The objective of the study was to examine the effect of Brahman genetics on collagen enzymatic crosslinking gene expression and meat tenderness. Steers were randomly selected to represent a high percentage Brahman genetics (n = 13), Half-Blood genetics (n = 13), Brangus genetics (n = 13), and a high percentage Angus genetics (n = 13). Muscle samples from ...
Cucchiarini M M Center of Experimental Orthopaedics, Saarland University Medical Center, Homburg/Saar, - - 2014
Direct therapeutic gene transfer is a promising tool to treat articular cartilage defects. Here, we tested the ability of an recombinant adeno-associated virus (rAAV) insulin-like growth factor I (IGF-I) vector to improve the early repair of cartilage lesions in vivo. The vector was administered for 3 weeks in osteochondral defects ...
Bagavandoss P P Department of Biological Sciences, Kent State University at Stark, 6000 Frank Avenue NW, North Canton, OH 44720, USA. Electronic address: - - 2014
Ovarian morphogenesis and physiology in mammals take place in the context of hormones, paracrine factors and extracellular matrix molecules. Both fibrillar type I collagen and the multidomain tenascin-C are matrix molecules capable of modulating the behavior of both normal and neoplastic cells in many organs. Therefore, the objective of this ...
Prasadam Indira I Medical Device Domain, Institute of Health and Biomedical Innovation, Queensland University of Technology and Orthopaedic Department, Prince Charles Hospital, Brisbane, Queensland, Brisbane, Australia. - - 2014
The aim of this study was to test the possible involvement, relevance and significance of dentin matrix protein 1 (DMP1) in chondrocyte redifferentiation and OA. To examine the function of DMP1 in vitro, bone marrow stromal cells (BMSCs) and articular chondrocytes (ACs) were isolated and differentiated in micromasses in the ...
Xue Meilang M Sutton Research Laboratory, Department of Rheumatology, Kolling Institute of Medical Research, University of Sydney at Royal North Shore Hospital, St Leonards, NSW, Australia and Bio-Safety Research Institute, Chonbuk National University, College of Veterinary Medicine, Jeonju, South Korea. - - 2014
The aim of this study was to investigate the effect of endogenous matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) on the invasive characteristics of RA synovial fibroblasts. Synovial fibroblasts isolated from patients with RA or OA were treated with MMP small interfering RNA (siRNA), inhibitors and recombinant proteins or ...
Dworatzek E E Institute of Gender in Medicine, Berlin, - - 2014
Female pressure-overloaded hearts show less collagen I and III gene expression and cardiac fibrosis, compared to males. This suggests an inhibitory effect of 17β-Oestradiol (E2) on collagens. Since E2 mediates its effect mainly via activation of its receptors, oestrogen receptor (ER) alpha (α) and beta (β), we analysed here the ...
Neill Thomas T Department of Pathology, Anatomy and Cell Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania; Cancer Cell Biology and Signaling Program, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, - - 2014
Autophagy plays an essential role in maintaining an intricate balance between nutrient demands and energetic requirements during normal homeostasis. Autophagy recycles metabolic substrates from nonspecific bulk degradation of proteins and excess or damaged organelles. Recent work posits an active and dynamic signaling role for extracellular matrix-evoked autophagic regulation, that is, ...
Wang L L National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS), National Institutes of Health (NIH), Bethesda, MD, USA - - 2014
A full understanding of the key regulators controlling periodontal development and homeostasis is necessary for the design of improved periodontal regenerative therapies. Small leucine-rich proteoglycans (SLRPs) are extracellular matrix molecules suggested to regulate collagen organization and cell signaling. Mice with double-deficiency of 2 SLRPs, fibromodulin and biglycan (dKO), acquire skeletal ...
Yuan Yue Y Institute of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, - - 2014
The tumor necrosis factor receptor-associated death domain protein (TRADD) regulates cell proliferation and apoptosis via tumor necrosis factor alpha (TNF-α)-mediated signaling pathways. Low levels of TRADD expression may result in the excessive proliferation of hypertrophic scar fibroblasts (HSFb). This study investigated the effects of a lentiviral vector carrying the human ...
Sun Congcong C Department of Respiratory Medicine, Qilu Hospital, Shandong University, Jinan, - - 2014
Emerging evidence suggests that the process of small airway remodeling is mediated by profibrotic growth factors produced by epithelium, which are capable of activating the underlying mesenchymal cells with excessive collagen production. It has been demonstrated that human cathelicidin antimicrobial protein LL-37 is highly expressed in small airway epithelium from ...
Xu Hong-Guang HG Department of Orthopedic Surgery, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui 241001, China. Electronic address: - - 2014
Calcification of end plate chondrocytes is a major cause of intervertebral disc (IVD) degeneration. However, the underlying molecular mechanism of end plate chondrocyte calcification is still unclear. The aim of this study was to clarify whether autophagy in end plate chondrocytes could protect the calcification of endplate chondrocytes. Previous studies ...
He Jing J Shaanxi R&D Centre of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, 710069, - - 2014
Type III collagen is one of the most abundant protein in the human body, which forms collagen fibrils and provides the stiff, resilient characteristics of many tissues. In this paper, a new method for secretory expression of recombinant hydroxylated human collagen α1(III) chain in Pichia pastoris GS115 was applied. The ...
Zhang Kairui K Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, - - 2014
NSAIDs are often ingested to reduce the pain and improve regeneration of tendon after tendon injury. Although the effects of NSAIDs in tendon healing have been reported, the data and conclusions are not consistent. Recently, tendon-derived stem cells (TDSCs) have been isolated from tendon tissues and has been suggested involved ...
Zhang Sihan S The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. - - 2014
The aim of the present study was to assess the association between chondrocytes and the extracellular matrix (ECM), and determine whether this contributes to osteoarthritis (OA). Chondrocyte hypertrophy was measured in articular cartilage samples from early-stage OA patients. In addition, rat chondrocytes were cultured and divided into four groups (A to D): ...
Lee So-Hyoung SH Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, South - - 2014
Candida albicans, the major human fungal pathogen, undergoes morphological transition from the budding yeast form to filamentous growth in response to nitrogen starvation. In this study, we identified a new function of GST2, whose expression was required for filamentous growth of C. albicans under nitrogen-limiting conditions. The Gst2p showed Gst ...
Cong Cuicui C Occupational Disease Department, Peking University Third Hospital, Beijing, - - 2014
ABSTRACT Silicosis is a serious occupational disease characterized by lung fibrosis that is caused by long-term inhalation of silica-containing fine particles. Lysophosphatidic acid (LPA) and LPA1/3 plays a role in lung fibrosis. Until recently, there has been little research investigating the role of LPA and LPA receptors (LPAR) in silica-induced ...
Hintermann Edith E Pharmazentrum Frankfurt / ZAFES, Johann Wolfgang Goethe University, Frankfurt am Main, - - 2014
Matrilins are a family of 4 oligomeric adaptor proteins whose functions in extracellular matrix assembly during pathophysiological events still need to be explored in more detail. Matrilin-2 is the largest family member and the only matrilin expressed in the naive liver. Several studies demonstrate that matrilin-2 interacts with collagen I, ...
Sakoguchi Akihito A Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan. - - 2014
The toll-like receptor (TLR) family is thought to be expressed in many cell types in the skin and play a role in various diseases. The expression pattern and role of TLRs in systemic sclerosis (SSc) is to be clarified. We investigated the expression profiles of TLR-related genes in SSc fibroblasts, ...
Zhao Xiang X University of Helsinki, - - 2014
The Amigo protein family consists of three transmembrane proteins characterized by six LRR (leucine-rich repeat) domains and one Ig (immunoglobulin-like) domain in their extracellular moieties. Previous in vitro studies have suggested a role as homophilic adhesion molecules in brain neurons but the in vivo functions remain unknown. Here we have ...
Tang Wenxin W National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China, - - 2014
Cotton fiber is a single cell that differentiates from the ovule epidermis and undergoes synchronous elongation with high secretion and growth rate. Apart from economic importance, cotton fiber provides an excellent single-celled model for studying mechanisms of cell-growth. Annexins are Ca(2+)- and phospholipid-binding proteins that have been reported to be ...
Wong Victor W VW From the *Division of Plastic and Reconstructive Surgery, Department of Surgery, Oregon Health and Science University, Portland, OR; and †Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University, Stanford, - - 2014
Excess scar formation after cutaneous injury can result in hypertrophic scar (HTS) or keloid formation. Modern strategies to treat pathologic scarring represent nontargeted approaches that produce suboptimal results. Mammalian target of rapamycin (mTOR), a central mediator of inflammation, has been proposed as a novel target to block fibroproliferation. To examine ...
An G G The Second Hospital of Harbin Medical University, Harbin, China. - - 2014
The multipotent mesenchymal stem cells (MSCs) were distributed in the bone marrow stroma, and could generate all of the different skeletal cell lineages. The osteogenic differentiation of MSCs, which is indicated by the increased alkaline phosphatase (ALP) activity and the enhanced accumulation of collagen, could be induced by a tetradecapeptide ...
Reynard Louise N LN Musculoskeletal Research Group, Institute of Cellular Medicine, 4th Floor Catherine Cookson Building, The Medical School, Framlington Place, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK, - - 2014
GDF5 encodes an extracellular signalling molecule that is essential for normal skeletal development. The rs144383 C to T SNP located in the 5'UTR of this gene is functional and has a pleiotropic effect on the musculoskeletal system, being a risk factor for knee-osteoarthritis (OA), congenital hip dysplasia, lumbar disc degeneration ...
Yang Bo B Laboratory of Biomechanics, Department of Anatomy, Third Military Medical University, Chongqing, - - 2014
MicroRNA-145 has been shown to regulate chondrocyte homeostasis. It seems that miR-145 is implicated in cartilage dysfunction in Osteoarthritis (OA). However, the functional role of miR-145 in interleukin-1 beta (IL-1β)-induced extracellular matrix (ECM) degradation of OA cartilage has never been clarified. Here, we show that miR-145 expression increased in OA ...
Guan Xueying X Department of Molecular Biosciences, Center for Computational Biology and Bioinformatics, and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712, - - 2014
Cotton is not only the most important source of renewal textile fibers, but also an excellent model for studying cell fate determination and polyploidy effects on gene expression and evolution of domestication traits. The combination of A and D-progenitor genomes into allotetraploid cotton induces intergenomic interactions and epigenetic effects, leading ...
Chen Wei W Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294; ypli@uab.edu - - 2014
The pathogenesis of cleidocranial dysplasia (CCD) as well as the specific role of core binding factor β (Cbfβ) and the Runt-related transcription factor (RUNX)/Cbfβ complex in postnatal skeletogenesis remain unclear. We demonstrate that Cbfβ ablation in osteoblast precursors, differentiating chondrocytes, osteoblasts, and odontoblasts via Osterix-Cre, results in severe craniofacial dysplasia, ...
Fischer Jennifer Maria JM Orthopaedic University Hospital Heidelberg, Research Center for Experimental Orthopaedics, Heidelberg, Germany ; - - 2014
Phenotype instability and pre-mature hypertrophy prevent the use of human mesenchymal stromal cells (MSC) for cartilage regeneration. Aim of this study was to investigate whether intermittent supplementation of parathyroid hormone-related protein (PTHrP), as opposed to constant treatment, can beneficially influence MSC chondrogenesis and to explore molecular mechanisms below catabolic and ...
Dabisch-Ruthe Mareike M Institut für Laboratoriums- und Transfusionsmedizin, Herz- und Diabeteszentrum Nordrhein-Westfalen, Universitätsklinik der Ruhr-Universität Bochum, Bad Oeynhausen, - - 2014
Pseudoxanthoma elasticum (PXE) is a rare hereditary disorder characterized by late onset and progressive calcification of elastic fibers in skin, eyes and the cardiovascular system, exemplifying a model for conditions characterized by soft tissue calcification. The aim of our study was to characterize cellular inorganic pyrophosphate (PPi) homeostasis in PXE. ...
Yi Xin X Department of Cardiology, Renmin Hospital of Wuhan University and Cardiovascular Research Institute of Wuhan University, Wuhan, Hubei 430060, P.R. - - 2014
Cardiac fibroblast (CF) proliferation and transformation into myofibroblasts play important roles in cardiac fibrosis during pathological myocardial remodeling. In this study, we demonstrate that hepatocyte growth factor (HGF), an antifibrotic factor in the process of pulmonary, renal and liver fibrosis, is a negative regulator of cardiac fibroblast transformation in response to transforming ...
Lindenmair Andrea A Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Donaueschingenstraße 13, 1200, Vienna, - - 2014
Human amniotic membrane (hAM) represents a tissue that is well established as biomaterial in the clinics with potential for new applications in regenerative medicine. For tissue engineering (TE) strategies, cells are usually combined with inductive factors and a carrier substrate. We have previously recognized that hAM represents a natural, preformed ...
Salvany Lara L Institute of Molecular and Cell Biology, Singapore 138673, - - 2014
The transcription cofactor MAL is regulated by free actin levels and thus by actin dynamics. MAL, together with its DNA-binding partner, SRF, is required for invasive cell migration and in experimental metastasis. Although MAL/SRF has many targets, we provide genetic evidence in both Drosophila and human cellular models that actin ...
Tian Fei F Shanghai Institute of Traumatology and Orthopedics, Ruijin Hospital, Jiao Tong University School of Medicine, Shanghai, 200025, People's Republic of China; Department of Pathology, University of Alabama at Birmingham, - - 2014
Core binding factor beta (Cbfβ) is essential for embryonic bone morphogenesis. Yet, the mechanisms by which Cbfβ regulates chondrocyte proliferation and differentiation as well as postnatal cartilage and bone formation remain unclear. Hence, using the paired-related homeobox transcription factor 1-Cre (Prx1-Cre) mice, mesenchymal stem cell-specific Cbfβ-deficient (Cbfβ(f/f) Prx1-Cre) mice were ...
Liu Weili W Department of Stress Medicine, Beijing Institute of Basic Medical Sciences, No. 27 Taiping Road, Haidian District, Beijing, 100850, - - 2014
Organ fibrosis has been viewed as a major medical problem that leads to progressive dysfunction of the organ and eventually the death of patients. Stress-related hormone norepinephrine (NE) has been reported to exert fibrogenic actions in the injured organ. Nix plays a critical role in pressure overload-induced cardiac remodeling and ...
Chun Qing Q Shanghai Burns Institute, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, - - 2014
The human hypertrophic scar undergoes hyperplasia and regression during progression. This study aimed to investigate whether fibroblasts in scar tissue undergo biological changes during the formation and regression of human hypertrophic scar. Using 32 scar samples, we measured collagen production by Masson's staining and the expression levels of transforming growth ...
Thakur Suresh S Division of Molecular Cardiology, Department of Medicine, College of Medicine, Texas A & M Health Science Center; Scott & White; Central Texas Veterans Health Care System, Temple, TX, - - 2014
Cardiac fibrosis is a final outcome of many clinical conditions that lead to cardiac failure and is characterized by a progressive substitution of cellular elements by extracellular-matrix proteins, such as collagen type I, collagen type II, connective tissue growth factor (CTGF), etc. AIM: : The aim of this study was ...
Karamichos D D Schepens Eye Research Institute/Massachusetts Eye and Ear and the Department of Ophthalmology Harvard Medical School, 20 Staniford Street, Boston, MA, USA. Electronic address: - - 2014
Corneal scarring following moderate to severe injury is inevitable. Despite significant advancements in the field, current treatments following these types of injuries are limited, and often, the visual recovery is poor. One of the problems and limitations is that corneal wound healing is a complex process, involving corneal cells, extracellular ...
Liu Sheng S 1Cincinnati Children's Hospital Medical - - 2014
Postnatal lung development requires coordination of three processes-surface area expansion, microvascular growth, and matrix remodeling. Since normal elastin structure is important for lung morphogenesis, since physiological remodeling of lung elastin has never been defined, and since elastin remodeling is angiogenic, we sought to test the hypothesis that during lung development ...
Madej W W Orthopaedic Research Laboratory, Radboud University Medical Centre, Nijmegen, The Netherlands. Electronic address: - - 2014
Transforming growth factor beta (TGF-β) in articular cartilage can signal via two routes, the ALK5/Smad2/3P and the ALK1/Smad1/5/8P route, the first being protective and the latter favouring chondrocyte terminal differentiation. Since biomechanical factors are known to play an essential role in osteoarthritis (OA) initiation and progression, we investigated if excessive ...
Han Xuesong X Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi׳an 710032, China; Department of Orthopedics, Fuzhou General Hospital of Nanjing, Command, PLA, Fuzhou 350025, - - 2014
Fibrochondrocytes are involved in entheses repair, but their response to mechanical strain (MS) is ill known. To determine if parathyroid hormone-related protein (PTHrP) expression in fibrochondrocytes from fibrocartilaginous entheses is modulated by MS, and to further observe the regulatory effects of human (h) PTHrP on fibrochondrocyte differentiation. Fibrochondrocytes from fibrocartilaginous ...
Li Ping P Ping Li, Ying Zhu, Yao-Yao Gong, Yun Wang, Ding Heng, Lin Lin, Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, - - 2014
To investigate whether resveratrol (3,4,5-trihydroxy-trans-stilbene) inhibits collagen I synthesis induced by insulin growth factor-1 (IGF-1) in intestinal fibroblasts, and to explore the possible molecular mechanisms. Male Sprague-Dawley rats were randomly divided into two groups: a control group and a 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis group. After 21 d of TNBS administration, the degree ...
Yazawa Masaki M Department of Plastic and Reconstructive Surgery, School of Medicine, Keio University, Tokyo, - - 2014
Reconstructive surgery for tumor resection, trauma, and congenital anomaly involves volume augmentation with autologous tissue transfer. However, a healthy region is damaged as a donor site, and the autologous tissue is transferred like a patchwork to the recipient site. We have attempted to induce adipogenesis activity in artificial biomaterial that ...
Zhang Zhe Z Department of Plastic Surgery, Peking University Third Hospital, Haidian, Beijing 100191, P.R. - - 2014
Periostin, a secreted extracellular matrix protein, is involved in the wound healing and pathological process of various human cancers. Keloid scars are fibroproliferative tumor-like lesions and develop under local hypoxia. Using suppression subtractive hybridization, in a previous study, we found that periostin is overexpressed in keloids compared with hypertrophic scars. ...
Luo Fayong - - 2014
Idiopathic pulmonary fibrosis (IPF) is a progressive disease of insidious onset, and is responsible for up to 30,000 deaths per year in the U.S. Excessive production of extracellular matrix by myofibroblasts has been shown to be an important pathological feature in IPF. TGF-beta1 is expressed in fibrotic lung and promotes ...
Wang Yanhua Y Department of Geriatrics, Peking University First Hospital, Xishiku Street No. 8, West District, Beijing, People's Republic of - - 2014
Platelet-derived growth factor-BB (PDGF-BB) and transforming growth factor-β1 (TGF-β1) are critically involved in idiopathic pulmonary fibrosis by inducing the proliferation and transdifferentiation of lung fibroblasts. In the present study, we examined the impact of diallyl disulfide (DADS), a garlic-derived compound, on such pathological conditions. DADS showed profound inhibitory effects on ...
Li Zhi Jiang ZJ Department of Food and Engineering, College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163-319, People's Republic of - - 2014
Various bioactivities of the starfish hatching enzyme (HE) including collagen gel contraction, MMPs activity, hydroxyproline release, and gene regulation based on the fibroblast-populated collagen lattice (FPCL) in three-dimensional medium were investigated for the improvement of scar and keloid. The starfish HE significantly inhibited the collagen gel contraction over 2 days of ...
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