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Yang-Hartwich Yang Y Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, - - 2015
p53 is a key transcriptional mediator that controls the expression of hundreds of target genes necessary to maintain cellular homeostasis and genome integrity. An important cellular function that is dependent on p53 transcriptional activity is apoptosis or programmed cell death. Indeed, inhibition of p53 transcriptional activity is often observed in ...
Ji Hong H Department of Paediatrics, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. - - 2014
The differentiation of fibroblasts, which are promoted by transforming growth factor-β (TGF-β)/Smad, is involved in the process of pulmonary fibrosis. The Rho/Rho-associated coiled-coil-forming protein kinase (Rock) pathway may regulate the fibroblast differentiation and myofibroblast expression of α-smooth muscle actin (α-SMA), however, the mechanism is not clear. The aim of the ...
Nikonorova Inna A IA Department of Cellular and Molecular Pharmacology, Rutgers Robert Wood Johnson Medical School, Piscataway, NJ 08854, - - 2014
TRPM7 is an essential and ubiquitous channel-kinase regulating cellular influx of Mg(2+). Although TRPM7 mRNA is highly abundant, very small amount of the protein is detected in cells, suggesting post-transcriptional regulation of trpm7 gene expression. We found that TRPM7 mRNA 5'-leader contains two evolutionarily conserved upstream open reading frames that ...
Moriuchi Takanobu T Graduate School of Life Science, University of Hyogo, Koto, Kamigori, Hyogo, 678-1297, - - 2014
Mutation of the lamin A gene (LMNA) causes a diverse range of diseases referred to as laminopathies. Because most laminopathies have a dominant inheritance pattern and progress gradually, cultured cells stably expressing mutant lamin A at the same level as endogenous wild-type cells are required for chronological analysis. In this ...
Vreeland Amanda C AC Case Western Reserve University, United - - 2014
Cellular retinoic acid-binding protein 2 (CRABP2) potently suppresses the growth of various carcinomas but the mechanism(s) that underlie this activity remain incompletely understood. CRABP2 displays two distinct functions: the classical function of this protein is to directly deliver retinoic acid (RA) to RAR, a nuclear receptor activated by this hormone, ...
Hua Brian L BL Massachusetts Institute of - - 2014
The mechanisms that underlie metazoan DNA replication initiation, especially the connection between transcription and replication origin activation, are not well understood. To probe the role of transcription in origin activation, we exploited a specific replication origin in Drosophila melanogaster follicle cells, ori62, which coincides with the yellow-g2 transcription unit and ...
Hallenborg Philip P University of Southern Denmark, - - 2014
Adipocyte differentiation is orchestrated by the ligand-activated nuclear receptor PPARγ. Endogenous ligands comprise oxidized derivatives of arachidonic acid and structurally similar polyunsaturated fatty acids. Although expression of PPARγ peaks in mature adipocytes, ligands are produced primarily at the onset of differentiation. Concomitant with agonist production murine fibroblasts undergo two rounds ...
Lichius Alexander A Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, - - 2014
Trichoderma reesei is a model for investigating the regulation of (hemi-)cellulase gene expression. Cellulases are formed adaptively, and the transcriptional activator XYR1 and the carbon catabolite repressor CRE1 are main regulators of their expression. We quantified the nucleo-cytoplasmic shuttling dynamics of GFP-fusion proteins of both transcription factors under cellulase and ...
Zhou X X Department of Biochemistry & Molecular Biology, Tulane Cancer Center; Tulane University School of Medicine; New Orleans, Louisiana, - - 2014
Over the past decade, a number of ribosomal proteins (RPs) have been found to have a role in activating the tumor suppressor p53 by directly binding to MDM2 and impeding its activity toward p53. Herein, we report that RPL5 and RPL11 can also enhance the transcriptional activity of a p53 ...
Soares Joana J REQUIMTE, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira n.° 164, 4050-313, Porto, Portugal; Laboratório de Microbiologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira n.° 164, 4050-313, Porto, - - 2014
One of the most appealing targets for anticancer treatment is the p53 tumor suppressor protein. In half of human cancers, this protein is inactivated due to endogenous negative regulators such as MDM2. Actually, restoring the p53 activity, particularly through the inhibition of its interaction with MDM2, is considered a valuable ...
Chuang Tsai-Der TD Department of Obstetrics and Gynecology, Harbor-UCLA Medical Center and LA Biomed, Torrance, CA, - - 2014
Uterine leiomyosarcoma is a relatively rare malignancy with high mortality due to metastasis and chemoresistance. Leiomyosarcomas share similar morphological characteristics with leiomyomas which are considered to have the potential of transformation into leiomyosarcoma. Accumulated evidence suggests that microRNAs acting as regulators of gene expression at the posttranscriptional level play key ...
R Costa Joana J Centre for Haematology, Department of Medicine, Hammersmith Hospital, Imperial College London, London, United - - 2014
A rare point mutation in the core promoter -270GC-rich box of PIGM, a housekeeping gene, disrupts binding of the generic transcription factor (TF) Sp1 and causes inherited glycosylphosphatidylinositol (GPI) deficiency (IGD). We show that while PIGM mRNA levels and surface GPI expression in IGD B cells are low, GPI expression ...
Koh Dong-In DI Brain Korea 21 Plus Project for Medical Sciences, Department of Biochemistry and Molecular Biology, Severance Biomedical Research Institute, Yonsei University School of Medicine, SeoDaeMoon-Ku, Seoul 120-752, - - 2014
An unresolved issue in genotoxic stress response is identification of induced regulatory proteins and how these activate tumor suppressor p53 to determine appropriate cell responses. Transcription factor KAISO was previously described to repress transcription following binding to methylated DNA. In this study, we show that KAISO is induced by DNA ...
Kiriyama Yuuka Y Department of Applied Biology, Shinshu University, - - 2014
Bacillus subtilis LytF plays a principal role in cell separation through its localisation at the septa and poles on the vegetative cell surface. In this study, we found that a mutation in a major lipoteichoic acid (LTA) synthase gene-ltaS-results in a considerable reduction in the σD-dependent transcription of lytF. Transcription ...
Wang Ling L Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy, Sichuan University, 610041, Chengdu, Sichuan, China, - - 2014
Increasing numbers of natural products have been found to possess anticancer effects. Nuclear factor erythroid-2-related factor-2 (Nrf2) is a master regulator of the antioxidative stress response, and our previous studies found that epigenetic modification of the Nrf2 gene appears to be a critical mechanism. Salvia miltiorrhiza, a Chinese herbal medicine ...
Peltonen Karita K Molecular Cancer Biology Program, University of - - 2014
Activation of the p53 pathway has been considered a therapeutic strategy to target cancers. We have previously identified several p53 activating small molecules in a cell-based screen. Two of the compounds activated p53 by causing DNA damage, but this modality was absent in the other four. We recently showed that ...
Fang Lei - - 2014
Cotton fiber, a highly elongated, thickened single cell of the seed epidermis, is a powerful cell wall research model. Fiber length, largely determined during the elongation stage, is a key property of fiber quality. Several studies using expressed sequence tags and microarray analysis have identified transcripts that accumulate preferentially during ...
Xu Xiao-Ling XL Department of Medical Oncology, Zhejiang Cancer Hospital, 38 Guangji Road, Hangzhou, - - 2014
The p53 protein is involved in many biological functions in cancer, such as cell cycle arrest, DNA repair, apoptosis, senescence, DNA metabolism, angiogenesis, and cellular differentiation. However, the association between p53 expression and clinicopathological findings or prognosis in esophageal squamous cell carcinoma (ESCC) is controversial. We designed a large-scale study ...
Chen Shu-Qin SQ *Department of Gynecology and Obstetrics, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; and †Department of Gynecology and Obstetrics, The People's Hospital of Guizhou Province, Guizhou, - - 2014
The current study was undertaken to investigate the predictive value of simultaneous enhancer of zeste homolog 2 (EZH2) and P53 expression in lesions of patients with cervical squamous cell carcinoma. Quantum dot double fluorescence staining was applied to detect EZH2 and P53 protein in biopsy tissue of 168 patients with ...
Ramdas Nisha M NM National Centre for Biological Sciences, TIFR, Bangalore, - - 2014
The nucleus is sculpted towards various morphologies during cellular differentiation and development. Alterations in nuclear shape often result in changes to chromatin organization and genome function. This is thought to be reflective of its role as a cellular mechanotransducer. Recent evidence has highlighted the importance of cytoskeletal organization in defining ...
Perriaud Laury L Institut Curie, Centre de Recherche, Bât. 110-112, Centre Universitaire, Orsay, F-91405, France Inserm, U612, Bât. 110-112, Centre Universitaire, Orsay, F-91405, - - 2014
G-quadruplex (G4) structures in intron 3 of the p53 pre-mRNA modulate intron 2 splicing, altering the balance between the fully spliced p53 transcript (FSp53, encoding full-length p53) and an incompletely spliced transcript retaining intron 2 (p53I2 encoding the N-terminally truncated Δ40p53 isoform). The nucleotides forming G4s overlap the polymorphism rs17878362 ...
DiMauro T T Department of Biochemistry and Molecular Pharmacology and NYU Cancer Institute, New York University School of Medicine, New York, NY, - - 2014
The Polycomb group protein Bmi-1 is an essential regulator of cellular senescence and is believed to function largely through the direct repression of the Ink4a/Arf locus. However, concurrent deletion of Ink4a/Arf does not fully rescue the defects detected in Bmi-1(-/-) mice, indicating that additional Bmi-1 targets remain to be identified. ...
Ichiyanagi Tomoko T Department of Immunology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Kita-ku, Okayama 700-8558, Japan Division of Epigenomics and Development, Medical Institute of Bioregulation, Kyushu University, Higashi-ku, Fukuoka 812-8582, - - 2014
HSP90, found in all kingdoms of life, is a major chaperone protein regulating many client proteins. We demonstrated that HSP90α, one of two paralogs duplicated in vertebrates, plays an important role in the biogenesis of fetal PIWI-interacting RNAs (piRNA), which act against the transposon activities, in mouse male germ cells. ...
Cadar Adrian G AG Division of Cardiovascular Medicine, Vanderbilt University School of Medicine, Nashville, TN, United States; Department of Molecular Physiology & Biophysics, Vanderbilt University School of Medicine, Nashville, TN, United - - 2014
Titin is the largest known protein and a critical determinant of myofibril elasticity and sarcomere structure in striated muscle. Accumulating evidence that mRNA transcripts are post-transcriptionally regulated by specific motifs located in the flanking untranslated regions (UTRs) led us to consider the role of titin 5'-UTR in regulating its translational ...
Wu Feili F College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, 22 Xinong Road, Yangling, Shaanxi 712100, - - 2014
Polychlorinated biphenyls (PCBs) are a group of environmental contaminants widely dispersed in aquatic system. Recent data have shown that Aroclor 1254 (a highly chlorinated PCB mixture) has the potential to induce oxidative stress. The antioxidant genes are usually up-regulated in response to the oxidative stress. However, the mechanisms underlying the ...
Ponsuksili Siriluck - - 2014
Endometrial receptivity is a prerequisite for successful embryo implantation and pregnancy. Receptivity involves complex processes promoted by many transcripts that are key components of molecular pathways which depend on ovarian hormones and that contribute to shaping structural, metabolic, and communication properties of endometrial cells towards reception of embryos. MicroRNAs (miRNAs) ...
Janaki Ramaiah M M Centre for Chemical Biology, Indian Institute of Chemical Technology (IICT), Tarnaka, Hyderabad, India; School of Chemical and Biotechnology, Sastra University, Tirumalaisamudram, Thanjavur, India. Electronic address: - - 2014
MicroRNAs are small non-coding RNAs that regulate post-transcriptional mRNA expression by binding to 3' untranslated region (3'-UTR) of the complementary mRNA sequence resulting in translational repression and gene silencing. They act as negative regulators of gene expression and play a pivotal role in regulating apoptosis and cell proliferation. Studies have ...
Lemay Jean-François JF 1] RNA Group, Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Québec, Canada. - - 2014
The exosome is an RNA-decay complex that constantly monitors transcription and contributes to post-transcriptional turnover of faulty mRNAs. Yet how nuclear RNA surveillance by the exosome is coordinated with transcription is still unknown. Here we show that the RNA exosome of Schizosaccharomyces pombe can target the transcription machinery by terminating ...
Espinosa L L Program in Cancer Research, Institut Hospital del Mar d'Investigacions Mèdiques (IMIM), Doctor Aiguader, 88, Barcelona 08003, - - 2014
The nuclear factor-κB (NF-κB) signalling pathway participates in a multitude of biological processes, which imply the requirement of a complex and precise regulation. IκB (for Inhibitor of kappaB) proteins, which bind and retain NF-κB dimers in the cytoplasm, are the main contributors to negative regulation of NF-κB under non-stimulation conditions. ...
Zou Xiao-Hui XH State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, - - 2014
The human adenovirus (HAdV) early protein E1B55K interacts with E4orf6 to form an E3 ubiquitin ligase complex, which plays key roles in virus replication. To illustrate the reason for the fastidiousness of HAdV-41 in 293 cells, interaction between heterotypic E1B55K and E4orf6 proteins was investigated. HAdV-5 E1B55K could interact with ...
Williams R R Department of Biological Sciences, University of Windsor, Windsor, Ontario, Canada - - 2014
The Brown bullhead (Ameiurus nebulosus) is able to survive and reproduce in high levels of environmentally contaminated areas of the Great Lakes. The purpose of this study was to establish whether there are adaptive genetic/molecular changes occurring in these fish that allow for their survival. Expression of a cell cycle ...
Marfil V V Unidad de Hepatología Experimental, CIBERehd, Instituto de Investigación Sanitaria La Fe, Avda. Fernando Abril Martorell 106, Valencia, - - 2014
c-Myc transcription factor is a key protein involved in cellular growth, proliferation and metabolism. c-Myc is one of the most frequently activated oncogenes, highlighting the need to identify intracellular molecules that interact directly with c-Myc to suppress its function. Here we show that Hhex is able to interact with the ...
Ye Rui R Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, - - 2014
The mammalian circadian clock is based on a transcription-translation feedback loop (TTFL) in which CLOCK and BMAL1 proteins act as transcriptional activators of Cryptochrome and Period genes, which encode proteins that repress CLOCK-BMAL1 with a periodicity of ∼24 h. In this model, the mechanistic roles of CRY and PER are ...
Chittaranjan Suganthi S Canada's Michael Smith Genome Sciences Centre, BC Cancer Agency, Vancouver, BC, - - 2014
The majority of oligodendrogliomas (ODGs) exhibit combined losses of chromosomes 1p and 19q and mutations of isocitrate dehydrogenase (IDH1-R132H or IDH2-R172K). Approximately 70% of ODGs with 1p19q co-deletions harbor somatic mutations in the Capicua Transcriptional Repressor (CIC) gene on chromosome 19q13.2. Here we show that endogenous long (CIC-L) and short ...
Trucco Lucas Daniel LD Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, - - 2014
KLF6 is a member of the Krüppel-like factor family of transcription factors, with diverse roles in the regulation of cell physiology, including proliferation, signal transduction, and apoptosis. Mutations or down-regulation of KLF6 have been described in several human cancers. In this work, we found that KLF6-knockdown resulted in the formation ...
Tecleab Awet A Memorial Sloan Kettering Cancer Center, United - - 2014
Ral GTPases are critical effectors of Ras, yet the molecular mechanism by which they induce malignant transformation is not well understood. In this manuscript, we found the expression of K-Ras, RalB, and sometimes RalA, but not AKT1/2 and c-Raf, to be required for maintaining low levels of p53 in human ...
Chai Jinyu J MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, - - 2014
Plant senescence is a highly regulated process that can be induced by a range of factors. The nonexpressor of pathogenesis-related genes 1 (npr1) mutant is defective in the salicylic acid (SA) signalling pathway, displaying delayed yellowing during developmental senescence. However, the regulating mechanism of NPR1 on exogenous SA-induced senescence in ...
Shi Guang G East China Normal University, - - 2014
Regulation of rDNA transcription is central to cell growth and proliferation. PHF2 and PHF8 are enriched in the nucleolus and belong to a subfamily of histone demethylases that also possess a PHD domain-dependent binding activity for di/tri-methylated histone 3 lysine 4 (H3K4me2/3). In this study, we show that, unlike PHF8 ...
Yan Chunxia C State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, - - 2014
The Tudor-SN protein (TSN) is universally expressed and highly conserved in eukaryotes. In Arabidopsis, TSN is reportedly involved in stress adaptation, but the mechanism involved in this adaptation is not understood. Here, we provide evidence that TSN regulates the mRNA levels of GA20ox3, a key enzyme for gibberellin (GA) biosynthesis. ...
Zhu Tianqing T Swedish University of Agricultural Sciences, Department of Plant Biology, Uppsala BioCenter, Linnean Center of Plant Biology in Uppsala, PO-Box 7080, SE-75007 Uppsala, Sweden - - 2014
Proper embryo development is crucial as that is when the primary body axes are established. In Arabidopsis, AtWOX8 and AtWOX9, members of the WUSCHEL-RELATED HOMEOBOX (WOX) gene family, are critical for embryo development. In Norway spruce, PaWOX8/9, which is expressed in embryos, is the homologue of AtWOX8 and AtWOX9. In ...
Zhao Tong T State Key Laboratory of Medicinal Chemical Biology, Collaborative Innovation Center for Biotherapy, 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics and College of Life Sciences, Nankai University, Tianjin, 300071, - - 2014
Non-coding RNAs (ncRNAs), such as microRNAs and large intergenic non-coding RNAs, have been shown to play essential roles in regulating pluripotency. Yet, it is not clear the role of natural antisense transcripts (NATs), also belonging to ncRNAs, in embryonic stem cells. However, the role of NATs in embryonic stem cells ...
Moon Heegyum H School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, - - 2014
The product of proto-oncogene Ron is a human receptor for the macrophage-stimulating protein (MSP). Upon activation, Ron is able to induce cell dissociation, migration and matrix invasion. Exon 11 skipping of Ron pre-mRNA produces Ronā–³165 protein that is constitutively active even in the absence of its ligand. Here we show ...
Patwardhan Gauri A GA Department of Basic Pharmaceutical Sciences, University of Louisiana at Monroe, Monroe, LA, - - 2014
Mutants of tumor suppressor p53 not only lose the activity in genome stabilizing and in tumor suppression, but also exhibit oncogenic function in cancer cells. Most efforts in restoring p53 biological activity focus on either altering mutant-protein conformation or introducing an exogenous p53 gene into cells to eliminate p53-mutant cancer ...
Akita Hirofumi H Department of Surgery, Osaka University, Graduate School of - - 2014
Activation of c-MYC is an oncogenic hallmark of many cancers including liver cancer, and is associated with a variety of adverse prognostic characteristics. Despite a causative role during malignant transformation and progression in hepatocarcinogenesis, consequences of c-MYC activation for the biology of hepatic cancer stem cells (CSCs) are undefined. Here, ...
Das Kausik K Research and Development Division, Streck Inc., 7002 S 109 Street, Omaha, NE, 68128, - - 2014
Messenger RNA (mRNA) expression levels in blood cells are important in disease diagnosis, prognosis and biomarker discovery research. Accurate measurements of intracellular mRNA levels in blood cells depend upon several pre-analytical factors, including delays in RNA extraction from blood after phlebotomy. Dramatic changes in mRNA expression levels caused by delays ...
Camozzi Daria D CNR Institute for Molecular Genetics; Unit of Bologna and SC Laboratory of Muscoskeletal Cell Biology; Rizzoli Orthopedic Institute; Bologna, - - 2014
Interconnected functional strategies govern chromatin dynamics in eukaryotic cells. In this context, A and B type lamins, the nuclear intermediate filaments, act on diverse platforms involved in tissue homeostasis. On the nuclear side, lamins elicit large scale or fine chromatin conformational changes, affect DNA damage response factors and transcription factor ...
Huang Eng-Yen EY Department of Radiation Oncology, Kaohsiung Chang Gung Memorial Hospital, 123 Ta-Pei Road, Niao-Sung District, Kaohsiung 833, Taiwan. Graduate Institute of Clinical Medical Sciences, Chang Gung University College of Medicine, Taiwan. School of Traditional Chinese Medicine, Chang Gung University College of Medicine, Taiwan. These authors contributed equally to this - - 2014
Amifostine (AM) is a radioprotector that scavenges free radicals and is used in patients undergoing radiotherapy. p53 has long been implicated in cell cycle arrest for cellular repair after radiation exposure. We therefore investigated the protective p53-dependent mechanism of AM on small bowel damage after lethal whole-abdominal irradiation (WAI). AM ...
Kizil Caghan C DFG-Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Fetscherstrasse 105, Dresden 01307, - - 2014
Canonical β-catenin-dependent Wnt signal transduction is important for several biological phenomena, such as cell fate determination, cell proliferation, stem cell maintenance and anterior-posterior axis formation. The hallmark of canonical Wnt signaling is the translocation of β-catenin into the nucleus where it activates gene transcription. However, the mechanisms regulating β-catenin nuclear ...
Shin Jun-Hye JH School of Biological Sciences, University of Missouri - Kansas City, Kansas City, Missouri, United States of - - 2014
The exosome complex functions in RNA metabolism and transcriptional gene silencing. Here, we report that mutations of two Arabidopsis genes encoding nuclear exosome components AtRRP6L1 and AtRRP6L2, cause de-repression of the main flowering repressor FLOWERING LOCUS C (FLC) and thus delay flowering in early-flowering Arabidopsis ecotypes. AtRRP6L mutations affect the ...
Zhang Bingqing B Department of Biology, Indiana University, Bloomington, Indiana, United States of - - 2014
Apoptotic cell death is an important response to genotoxic stress that prevents oncogenesis. It is known that tissues can differ in their apoptotic response, but molecular mechanisms are little understood. Here, we show that Drosophila polyploid endocycling cells (G/S cycle) repress the apoptotic response to DNA damage through at least ...
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