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Hu Yue Y Department of Clinical Laboratory, The Second People's Hospital of Hefei, Hefei City, - - 2014
Globally, 15-20 million people are infected with Clonorchis sinensis (C. sinensis) which results in clonorchiasis. In China, clonorchiasis is considered to be one of the fastest-growing food-borne parasitic diseases. That more key molecules of C. sinensis are characterized will be helpful to understand biology and pathogenesis of the carcinogenic liver ...
Nagarajan Sabarish S Department of Biochemistry and Molecular Biology, and Clark H. Smith Brain Tumour Centre, Southern Alberta Cancer Research Institute, University of Calgary, HRIC, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, - - 2014
The larval period of the Drosophila life cycle is characterized by immense growth. In nutrient rich conditions, larvae increase in mass approximately two hundred-fold in five days. However, upon nutrient deprivation, growth is arrested. The prevailing view is that dietary amino acids drive this larval growth by activating the conserved ...
Shen Guanwang G State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, - - 2014
The vertebrate estrogens include 17-β-estradiol (E2), which has an analog in silkworm ovaries. In this study, the Bombyx mori vitellogenin gene (BmVg) was used as a biomarker to analyze the function of the E2 in silkworm. In most oviparous animals, Vg has female-specific expression. However, BmVg expression was also detected ...
Banerjee Anirban A Fish Biology Research Unit, Department of Zoology, School of Life Sciences, Visva-Bharati University, Santiniketan, 731235, West Bengal, - - 2014
In present study, a microcosm experiment is carried out to investigate the efficacy of 120 and 250-ppm crude aqueous extract of Azadirachta leaf on oocyte maturation, oviposition, embryonic development and hatching of the eggs of a fish ectoparasite Argulus bengalensis. Relative abundance of different maturing oocyte stages in the ovary ...
Mommens Maren - - 2014
Commercial Atlantic halibut (Hippoglossus hippoglossus) farming is restricted by variable oocyte quality, slow growth, and early maturation of male fish. Maternally transferred components regulate early developmental processes; therefore, they have an effect on the future viability of the embryo. Using a newly developed Agilent 10 k custom-made oligonucleotide array, we ...
Morin Scott J SJ Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, Durham, NH, 03824-3544, - - 2014
We cloned two cDNAs for two gonadotropin-releasing hormones, GnRH2 (chicken GnRH-II) and GnRH3 (salmon GnRH), respectively, from the black sea bass (Centropristis striata). Black sea bass are protogynous hermaphroditic teleosts that change from females to males between 2 and 5 years of age. Similar to other GnRH precursors, the precursors of ...
Nishio Shunsuke - - 2014
Vertebrate eggs are surrounded by an egg coat, which is a specific extracellular egg matrix consisting of several glycoproteins with a conserved zona pellucida (ZP) domain. Two mammalian egg coat subunits, ZP2 and ZP3, have been suggested to act as sperm receptors. In bird eggs, however, ZP2 has never been ...
Zhu Bin B College of Animal Science and Technology - - 2014
Abstract Carbon nanotubes (CNTs) are widely used in industrial and commercial applications, but few studies systematically evaluate their developmental toxicity on aquatic organism. Using rare minnow (Gobiocypris rarus) at early life stages as experimental models, developmental toxicity of functionalized single-walled CNTs (SWCNTs) was investigated following exposure to 0-320 mg/L for 144 h. ...
Thongda Willawan W Institute of Marine and Environmental Technology, 701 East Pratt Street, Columbus Center, Baltimore, MD 21202, - - 2014
In general, season affects the physiology and behavior of most animals. Warmer temperatures accelerate growth and reproduction of ectotherms, whereas these processes are slowed or halted in colder temperatures. Female blue crabs, Callinectes sapidus inhabiting the Chesapeake Bay, exhibit a seasonal migratory behavior that is closely tied with spawning and ...
Mohamat-Yusuff Ferdaus - - 2014
The morphological expressions and histopathological analysis of the gonads of a tropical marine neogastropod species (Thais sp.) from East Malaysia revealed new evidence of mechanical sterility in the imposex affected females. The gradual development of imposex was classified into five stages (Stage 0 to Stage 4) with three types of ...
Mukherjee Krishnendu - - 2014
MicroRNAs (miRNAs) are small non-coding RNAs that act as key players in the post-transcriptional regulation of protein synthesis. Although little is known about their role in complex physiological processes such as development and immunity, our knowledge is expanding rapidly thanks to the use of model systems. The larvae of the ...
Vandersmissen Hans Peter HP KU Leuven, Animal Physiology and Neurobiology, Naamsestraat 59, PO Box 2465, Leuven, Belgium. Electronic address: - - 2014
G protein-coupled receptors (GPCRs) play key roles in a wide diversity of physiological processes and signalling pathways. The leucine-rich repeats containing GPCRs (LGRs) are a subfamily that is well-conserved throughout most metazoan phyla and have important regulatory roles in vertebrates. Here, we report on the critical role of Drosophila melanogaster ...
Kong B Y BY Department of Obstetrics and Gynecology, Northwestern University, Feinberg School of Medicine, 250 East Superior Street, Suite 3-2303, Chicago, IL - - 2014
Rapid cellular zinc influx regulates early mammalian development during the oocyte-to-egg transition through modulation of the meiotic cell cycle. Despite the physiological necessity of this zinc influx, the molecular mechanisms that govern such accumulation are unknown. Here we show that the fully-grown mammalian oocyte does not employ a transcriptionally-based mechanism ...
Tchourine Konstantine K Center for Genomics and Systems Biology, New York University, New York, USA. - - 2014
Cells respond to environmental stimuli with expression changes at both the mRNA and protein level, and a plethora of known and unknown regulators affect synthesis and degradation rates of the resulting proteins. To investigate the major principles of gene expression regulation in dynamic systems, we estimated protein synthesis and degradation ...
Fu Kai-Yun KY Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: - - 2014
Vacuolar-type H(+)-ATPases (vATPases) are localized in the apical membranes of nearly all epithelial tissues of insects, energize the membranes to absorb and/or secrete ions and fluids, and play essential roles in many physiological functions. Here we cloned and characterized a 1041-bp full-length vATPase subunit E cDNA (named as LdATPaseE) that ...
Lin Chia-Hsien CH Department of Health Industry Management, School of Health Care Management, Kainan University, Taoyuan, - - 2014
The metal nickel (Ni(2+) ) is found everywhere in our daily lives, including coins, costume jewelry, and even nuts and chocolates. Nickel poisoning can cause inflammatory reactions, respiratory diseases, and allergic contact dermatitis. To clarify the mechanism by which nickel induces mediators of inflammation, we used the human acute monocytic ...
Pei Dan D School of Life Sciences, Tianjin Normal University, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin, 300387, China, - - 2014
The genes coding for wheat ATG4 and ATG8 were cloned and their roles in autophagy were verified. Implications of ATG4/ATG8 in wheat responses to stresses were suggested by expression profiling. Autophagy-related proteins ATG4 and ATG8 are crucial for autophagy biogenesis. ATG4 processes ATG8 precursor to expose its C-terminal glycine for ...
Xu Minhui M Department of General Surgery, the Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou 215001, China; Tumor Immunology and Gene Therapy Center, Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai 200438, - - 2014
To evaluate the prognostic significance of the epithelial cell adhesion molecule (EpCAM) expression in HCC, the relationship between EpCAM with ductular reactions and other clinical features. From 2001 to 2003, 106 HCC patients who underwent curative resection in Eastern Hepatobiliary Hospital were enrolled. The expression of EpCAM in tumoral tissues ...
Zhao Xue-Ming XM Embryo Biotechnology and Reproduction Laboratory,Institute of Animal Science (IAS),Chinese Academy of Agricultural Sciences (CAAS),no. 2 Yuanmingyuan Western Road,Haidian District,Beijing 100193,P.R. - - 2014
Summary This study was designed to determine the effect of melatonin on the in vitro maturation (IVM) and developmental potential of bovine oocytes denuded of the cumulus oophorus (DOs). DOs were cultured alone (DOs) or with 10-9 M melatonin (DOs + MT), cumulus-oocyte complexes (COCs) were cultured without melatonin as ...
Proff P P Department of Orthodontics, University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, - - 2014
The goal of the study was to examine the effects of a mechanical (orthodontic force simulation by static compressive loading) and a bacterial (endotoxins from a heat-inactivated gram-negative periodontal pathogen) stressor on the expression patterns of factors that are key to regulating osteoclastogenesis and bone remodeling. Three experimental groups were ...
Xu Hong-Fei HF Department of Forensic Medicine, Soochow University, Suzhou Dushuhu High Educational Town, Suzhou, Jiangsu 215123, P.R. - - 2014
MicroRNAs (miRNAs) comprise a broad class of small non‑coding RNAs that control the expression of complementary target messenger RNAs. The dysregulation of miRNAs by several mechanisms has been described in various disease states, including cardiac disease. Although an etiological link between viral myocarditis (VMC) and idiopathic dilated cardiomyopathy (DCM) has ...
Suchy Dariusz D Department of Internal Medicine and Clinical Pharmacology, Medical University of Silesia in Katowice, Medyków 18, 40752, Katowice, Poland, - - 2014
Macrophages are crucial for the development of atherosclerotic plaques. Classically activated macrophages contribute to plaque growth and destabilization, while alternatively activated macrophages increase plaque stability. Here, we assessed the influence of ezetimibe on the activation of monocyte-derived macrophages isolated from patients with hypercholesterolemia (total cholesterol 263.4 ± 12.5 mg/dl, low-density lipoprotein cholesterol 179.7 ± 11.3 mg/dl, ...
Rajendran Senthilkumar - - 2014
Plants respond differently to mechanical wounding and herbivore attack, using distinct pathways for defense. The versatile sweet potato sporamin possesses multiple biological functions in response to stress. However, the regulation of sporamin gene expression that is activated upon mechanical damage or herbivore attack has not been well studied. Biochemical analysis ...
Schweikhard Volker V Departments of Biology, Chemistry, Structural Biology, and Applied Physics, Stanford University, Stanford, CA - - 2014
Recent evidence suggests that transcript elongation by RNA polymerase II (RNAPII) is regulated by mechanical cues affecting the entry into, and exit from, transcriptionally inactive states, including pausing and arrest. We present a single-molecule optical-trapping study of the interactions of RNAPII with transcription elongation factors TFIIS and TFIIF, which affect ...
Wang Feng F Department of Cardiology, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai, 201102, - - 2014
As a well-known transcription factor, TBX5 is involved in embryonic cardiac development. Although TBX5 functions in a dose-dependent manner, the posttranscriptional regulation of human TBX5 is poorly understood. Thus, this study aimed to identify microRNAs that modulate TBX5 expression. Luciferase assays were used to screen miRNAs predicted to modulate TBX5 ...
Matsumoto David D Department of Psychology, San Francisco State - - 2014
Most studies on judgments of facial expressions of emotion have primarily utilized prototypical, high-intensity expressions. This paper examines judgments of subtle facial expressions of emotion, including not only low-intensity versions of full-face prototypes but also variants of those prototypes. A dynamic paradigm was used in which observers were shown a ...
Sidaway-Lee Kate - - 2014
Sensing and responding to ambient temperature is important for controlling growth and development of many organisms, in part by regulating mRNA levels. mRNA abundance can change with temperature, but it is unclear whether this results from changes in transcription or decay rates, and whether passive or active temperature regulation is ...
Paulus Andrea A a Department of Psychology , Saarland University , Saarbrüken , - - 2014
It has been suggested that the impact of emotional expressions on the startle reflex is influenced by the intention communicated by the expression (e.g., the intention to attack). However, we propose that the meaning of an emotional expression is not only based on the intention, but is also influenced by ...
Gorgoni Barbara B *Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, - - 2014
Gene expression can be regulated by a wide variety of mechanisms. One example concerns the growing body of evidence that the protein-production rate can be regulated at the level of translation elongation by controlling ribosome flux across the mRNA. Variations in the abundance of tRNA molecules cause different rates of ...
Zhi Xiaofei X Division of Gastrointestinal Surgery, Department of General Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing, - - 2014
Intestinal ischemic injury is a significant clinical problem arising from diseases or as a complication of abdominal surgery. Our previous study showed aquaporin 3 is involved in intestinal barrier impairment. Here, we revealed that intestinal ischemia induced a time-dependent increase of miR-874 expression and a time-dependent decrease of AQP3 expression, ...
Wongpalee Somsakul Pop SP Department of Immunology and Molecular Genetics, Molecular Biology Institute, University of California, Los Angeles, CA, - - 2014
In eukaryotic organisms, nascent transcripts of protein-coding genes contain intronic sequences that are not present in mature mRNAs. Pre-mRNA splicing removes introns and joins exons to form mature mRNAs. It is catalyzed by a large RNP complex called the spliceosome. Sequences within the pre-mRNA determine intron recognition and excision. This ...
Gray Jesse M JM Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of - - 2014
mRNA synthesis, processing, and destruction involve a complex series of molecular steps that are incompletely understood. Because the RNA intermediates in each of these steps have finite lifetimes, extensive mechanistic and dynamical information is encoded in total cellular RNA. Here we report the development of SnapShot-Seq, a set of computational ...
Gebauer Niklas N Institut für Pathologie, Universität zu Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Deutschland. - - 2014
The mechanisms promoting malignant transformation from chronic Helicobacter pylori-gastritis to gastric extranodal marginal zone lymphoma (MALT lymphoma) are insufficiently characterized. This follow-up study aimed to validate candidate microRNAs (miRs) in the process of neoplastic transformation. MicroRNA expression signatures (n=20) were generated for a total of 60 cases of gastric lesions ...
Liu Xun X Division of Pharmacology and Toxicology, School of - - 2014
The prevalence of HIV-associated neurocognitive disorders (HAND) remains high in patients infected with HIV-1. The production of pro-inflammatory cytokines by astrocytes/microglia exposed to viral proteins is thought to be one of the mechanisms leading to HIV-1- mediated neurotoxicity. In the present study we examined the effects of Nef on CCL5 ...
Sawada Reiko R Primate Research Institute, Kyoto University, Inuyama, Aichi, - - 2014
Previous studies have shown that females and males differ in the processing of emotional facial expressions including the recognition of emotion, and that emotional facial expressions are detected more rapidly than are neutral expressions. However, whether the sexes differ in the rapid detection of emotional facial expressions remains unclear. We ...
Arreaza Alven J AJ Dental Therapeutics, School of Dentistry, Central University of Venezuela, Caracas 1051, - - 2014
Oral lichen planus and lichenoid reactions are autoimmune type inflammatory conditions of the oral mucosa with similar clinical and histological characteristics. Recent data suggest that oral lichenoid reactions (OLR) present a greater percentage of malignant transformation than oral lichen planus (OLP). To compare the expression of bcl-2 and COX-2 in ...
Melo Carlos A CA Division of Gene Regulation, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The - - 2014
RNA-induced silencing complex is the cytoplasmic effector machine of the microRNA (miRNA) pathway and contains a single-stranded miRNA guiding it to its target mRNAs. The biogenesis of mature miRNAs is a regulatory process achieved by complex machinery composed of few protein components, among which the ribonuclease III Dicer plays a ...
Rio Donald C - - 2014
This protocol describes how to generate and analyze products and intermediates in a pre-mRNA splicing reaction. The reaction relies on the use of labeled, capped, synthetic pre-mRNAs, prepared by in vitro transcription, and Drosophila Kc cell culture nuclear extracts. The pre-mRNA substrate is incubated in the nuclear extract under splicing ...
Kotzer-Nevo Hani H Department of Genetics, the Hebrew University of Jerusalem, Jerusalem 91904, Israel. - - 2014
When isolated from mammalian cell nuclei, all nuclear pre-mRNAs are packaged in multi-subunit large ribonucleoprotein complexes-supraspliceosomes-composed of four native spliceosomes interconnected by the pre-mRNA. Supraspliceosomes contain all five spliceosomal U snRNPs, together with other splicing factors, and are functional in splicing. Supraspliceosomes studied thus far represent the steady-state population of ...
Zandi Mohammad Reza MR Department of Animal Sciences, School of Agriculture, Shiraz University, Shiraz, - - 2014
Melanocortin- 4 receptor (MC4R) and agouti- related peptide (AgRP) are involved in energy homeostasis in rats. According to MC4R and AgRP effects on luteinizing hormone (LH) secretion, they may influence the estrous cycle of rats. Therefore, the aim of this study was to investigate the expression of MC4R and AgRP ...
Zhu Jun J University of California, San Francisco, United - - 2013
The late steps of cholesterol biosynthesis are oxygen demanding requiring eleven oxygen molecules per synthesized cholesterol molecule. A key enzymatic reaction, which occurs at the top of the Bloch and Kandutsch-Russell pathways, is the demethylation of lanosterol and dihydrolanosterol. This reaction is catalyzed by lanosterol 14α demethylase (CYP51) and requires ...
Liu Min Ting MT Department of Chemistry, The City College of New York, The City University of New York, New York, NY 10031, - - 2013
The 3' end formation of mammalian pre-mRNA contributes to gene expression regulation by setting the downstream boundary of the 3' untranslated region, which in many genes carries regulatory sequences. A large number of protein cleavage factors participate in this pre-mRNA processing step, but chemical tools to manipulate this process are ...
Reinholt Sarah J SJ Department of Biological and Environmental Engineering, Cornell University , Ithaca, NY 14853, United - - 2013
The major modules for realizing molecular biological assays in a micro-total analysis system (μTAS) were developed for the detection of pathogenic organisms. The specific focus was the isolation and amplification of eukaryotic mRNA within a simple, single-channel device for very low RNA concentrations that could then be integrated with detection ...
Gruber Andreas R - - 2013
Expression of mature messenger RNAs (mRNAs) requires appropriate transcription initiation and termination, as well as pre-mRNA processing by capping, splicing, cleavage, and polyadenylation. A core 3'-end processing complex carries out the cleavage and polyadenylation reactions, but many proteins have been implicated in the selection of polyadenylation sites among the multiple ...
Paulus Andrea - - 2013
It has been repeatedly stated that approach and avoidance reactions to emotional faces are triggered by the intention signalled by the emotion. This line of thought suggests that each emotion signals a specific intention triggering a specific behavioural reaction. However, empirical results examining this assumption are inconsistent, suggesting that it ...
Chaitanya V S - - 2013
Cortisol levels in the circulation and at the sites of peripheral inflammation regulate Type 1 (Reversal) reactions in leprosy akin to delayed type hypersensitivity reactions (DTH). In this study we determine the extent to which the differential mRNA expression of genes encoding Cortisone-Cortisol shuttle enzymes (11 β Hydroxysteriod Dehydrogenase I ...
Pavlidou Anastasia - - 2013
Survivin is a novel antiapoptotic gene, which is a member of the inhibitor of apoptosis protein (IAP) family. Recently, 3 splice variants of this gene were cloned and characterized. This study aimed to validate a sensitive and specific method for the detection of survivin variants in breast cancer. Real-time quantitative ...
Cheung Lily S LS Department of Chemical and Biological Engineering and Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ - - 2013
Although it is widely appreciated that a typical developmental control gene is regulated by multiple enhancers, coordination of enhancer activities remains poorly understood. We propose a mechanism for such coordination in Drosophila oogenesis, when the expression of the transcription factor Broad (BR) evolves from a uniform to a two-domain pattern ...
Colwell Ginny - - 2013
Increasing the probability of getting promoted usually starts long before the job advertisement appears.
Kamoun Malek M Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA. Electronic address: - - 2013
Protein phosphorylation and dephosphorylation are both important for multiple steps in the splicing pathway. Members of the PP1 and PP2A subfamilies of phospho-serine/threonine phosphatases play essential but redundant roles in the second step of the splicing reaction. PP6, a member of the PP2A subfamily, is the mammalian homolog of yeast ...
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