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Haas Beth L BL Department of Chemistry, University of Michigan, Ann Arbor, MI - - 2014
Vibrio cholerae causes the human disease cholera by producing a potent toxin. The V. cholerae virulence pathway involves an unusual transcription step: the bitopic inner-membrane proteins TcpP and ToxR activate toxT transcription. As ToxT is the primary direct transcription activator in V. cholerae pathogenicity, its regulation by membrane-localized activators is key in ...
Weaver Keith E KE Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD 57069, Phone: 1-605-658-6381. Electronic address: - - 2014
The pAD1 par determinant was the first Type I toxin-antitoxin system identified in Gram-positive bacteria and has recently been shown to be the prototype of a family of loci that is widespread in these organisms. All family members have i) convergently transcribed toxin message and regulatory RNAs, ii) three non-contiguous ...
Hamarneh Sulaiman R SR *Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA; and †Sanford Children's Health Research Center, Sanford-Burnham Medical Research Institute, La Jolla, - - 2014
To determine the role of intestinal alkaline phosphatase (IAP) in enteral starvation-induced gut barrier dysfunction and to study its therapeutic effect as a supplement to prevent gut-derived sepsis. Critically ill patients are at increased risk for systemic sepsis and, in some cases, multiorgan failure leading to death. Years ago, the ...
Roberts Thomas C TC Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, - - 2014
Long non-coding RNAs (lncRNAs) are transcripts with low protein-coding potential that represent a large proportion of the transcriptional output of the cell. Many lncRNAs exhibit features indicative of functionality including tissue-restricted expression, localization to distinct subcellular structures, regulated expression and evolutionary conservation. Some lncRNAs have been shown to associate with ...
Tan Jianzhuang J State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University,Beijing100193,People's Republic of - - 2014
The present study investigated the effects of dietary arginine (Arg) supplementation on intestinal structure and functionality in broiler chickens subjected to coccidial challenge. The present study was a randomised complete block design employing a 3 × 2 factorial arrangement (n 8) with three dietary concentrations of Arg (11·1, 13·3 and 20·2 g/kg) with ...
Xu Gaosi - - 2014
BackgroundIt is unclear to what extent uremic toxins participate in inflammatory responses and the activities of deiodinases, as well as the effects of deiodinases on inflammatory cytokines.Materials and methodsHepatocellular carcinoma cell lines (HepG2) were transfected with small interfering ribonucleic acid (siRNA) specific for deiodinase type 1 (DIO1) and cultured with ...
Liu Lan L Cell Biology Group, Department of Surgery, University of Maryland School of Medicine, Maryland 21201 Veterans Affairs Medical Center, Baltimore, Maryland - - 2014
Inhibition of growth of the intestinal epithelium, a rapidly self-renewing tissue, is commonly found in various critical disorders. The RNA-binding protein HuR is highly expressed in the gut mucosa and modulates the stability and translation of target mRNAs, but its exact biological function in the intestinal epithelium remains unclear. Here, ...
Drozdzik Marek - - 2014
Intestinal transporters are crucial determinants in the oral absorption of drugs. We therefore studied mRNA expression (N=33) and absolute protein content (N=10) of clinically relevant transporters in healthy epithelium of the duodenum, proximal and distal jejunum and ileum and the ascending, transversal, descending and sigmoidal colon of six organ donors ...
Kumar Anoop A University of Illinois at - - 2014
SLC26A3 or DRA (Down Regulated in Adenoma) plays a major role in mediating Cl- absorption in the mammalian intestine. Disturbances in DRA function and expression have been implicated in intestinal disorders such as congenital chloride diarrhea and gut inflammation. Our previous studies showed that increase in DRA function and expression ...
Shi Changgui C Department of Orthopedics, Changzheng Hospital, the Second Military Medical University of China, Shanghai, China. Electronic address: - - 2014
Glucocorticoids act on the osteoblasts to up-regulate the expression of RANKL, which is very important in the etiology of glucocorticoids-induced osteoclasts differentiation and bone resorption. The mechanisms of this process are still not completely understood. Recent studies have shown that glucocorticoids mediate osteoblast function by decreasing the expression of microRNA-17~92a ...
Gong Qiuming Q Knight Cardiovascular Institute, Oregon Health & Science University, Portland, OR - - 2014
The KCNH2 gene encodes the Kv11.1 potassium channel that conducts the rapidly activating delayed rectifier current in the heart. KCNH2 pre-mRNA undergoes alternative processing; intron 9 splicing leads to the formation of a functional, full-length Kv11.1a isoform, while polyadenylation within intron 9 generates a non-functional, C-terminally truncated Kv11.1a-USO isoform. The ...
Tollini Laura A LA Department of Radiation Oncology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7512, USA; Lineberger Comprehensive Cancer Center, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7512, USA; Curriculum in Genetics and Molecular Biology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7512, - - 2014
Mdm2 E3 ubiquitin ligase-mediated p53 degradation is generally accepted as the major mechanism for p53 regulation; nevertheless, the in vivo significance of this function has not been unequivocally established. Here, we have generated an Mdm2(Y487A) knockin mouse; Mdm2(Y487A) mutation inactivates Mdm2 E3 ligase function without affecting its ability to bind its ...
Lee Heon-Jin HJ Department of Oral Microbiology and Immunology, School of Dentistry, Kyungpook National University, Daegu 700-412, Korea Brain Science and Engineering Institute, Kyungpook National University, Daegu 700-412, Korea - - 2014
MicroRNAs (miRNAs) have been shown to be major regulators of eukaryotic gene expression. Traditionally, miRNAs were thought to control highly complex signal transduction and other biological pathways by targeting coding transcripts, accounting for their important role in cellular events. Traditional miRNA biogenesis and function focused on several key enzymes that ...
Shu Hu H School of Life Sciences, Guangzhou University, Guangzhou 510006, China. Electronic address: - - 2014
The peptide QRFP plays an important role in the regulation of vertebrate feeding behavior. In this study, we cloned the full length cDNA of a QRFP precursor in a teleost fish, the orange-spotted grouper (Epinephelus coioides). Sequence analysis shown that the functional regions of QRFP in other vertebrates (QRFP-25 and ...
Ye Song S Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, No.79 Qingchun Road, Hangzhou, 310003, Zhejiang, People's Republic of - - 2014
Altered expressions of microRNAs (miRNAs) are reported in pancreatic cancer and associate with cancer pathogenesis, apoptosis, and cell growth, thereby functioning as either tumor suppressors or oncogenes. However, the majority of studies focus on defining the regulatory functions of miRNAs, whereas few investigations are directed toward assessing how the miRNA ...
Noh Haneul - - 2014
Little is known about the relationship between miRNA and mRNA expression in Alzheimer's disease (AD) at early- or late-symptomatic stages. Sequence-based target prediction algorithms and anti-correlation profiles have been applied to predict miRNA targets using omics data, but this approach often leads to false positive predictions. Here, we applied the ...
Hamid Fursham M FM *School of Biological Sciences, Nanyang Technological University, - - 2014
Higher eukaryotes rely on AS (alternative splicing) of pre-mRNAs (mRNA precursors) to generate more than one protein product from a single gene and to regulate mRNA stability and translational activity. An important example of the latter function involves an interplay between AS and NMD (nonsense-mediated decay), a cytoplasmic quality control ...
Hurst Laurence D - - 2014
BackgroundConventional wisdom holds that, owing to the dominance of features such as chromatin level control, the expression of a gene cannot be readily predicted from knowledge of promoter architecture. This is reflected, for example, in a weak or absent correlation between promoter divergence and expression divergence between paralogs. However, an ...
Behringer Carina C Technische Universitat - - 2014
The transcription of the Arabidopsis thaliana GATA transcription factors GNC and GNL/CGA1 is controlled by several growth regulatory signals including light and the phytohormones auxin, cytokinin, and gibberellin. To date, GNC and GNL have been attributed functions in the control of germination, greening, flowering time, floral development, senescence, and floral ...
Liu Xuehui X State Key Laboratory of Cardiovascular Disease, Division of Population Genetics, Fuwai Hospital & National Center of Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College & National Human Genome Center at Beijing, Beijing, - - 2014
-Hyperhomocysteinemia is a risk factor for cardiovascular disease. Coactivator- associated arginine methyltransferase 1 (CARM1) participates in the synthesis of homocysteine, but whether the genetic variations regulate CARM1 expression and homocysteine levels remains unknown. -Functional analyses combined with an association study were conducted to identify the causal variant for CARM1 expression ...
Faso Daniel J DJ School of Behavioral and Brain Sciences, The University of Texas at Dallas, GR41, Richardson, TX, 75080, - - 2014
Though many studies have examined facial affect perception by individuals with autism spectrum disorder (ASD), little research has investigated how facial expressivity in ASD is perceived by others. Here, naïve female observers (n = 38) judged the intensity, naturalness and emotional category of expressions produced by adults with ASD (n = 6) and typically ...
van Loosdregt Jorg J Division of Pediatrics, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, The Netherlands; Department of Cell Biology, University Medical Center Utrecht, Utrecht, The - - 2014
Forkhead box (FOX)P3 is a requisite transcription factor for the development and maintenance of immunosuppressive function of regulatory T (Treg) cells, and therefore for immune homeostasis. Post-translational modifications (PTMs) can transiently alter the functionality of transcription factors, and recent evidence reveals that FOXP3 can be regulated by various PTMs including ...
Fukazawa Jutarou J Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan RIKEN Plant Science Center, Tsurumi, Yokohama, Kanagawa 230-0045, Japan Department of Biological Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan - - 2014
Gibberellins (GAs) are essential regulators of plant development, and DELLAs are negative regulators of GA signaling. The mechanism of GA-dependent transcription has been explained by DELLA-mediated titration of transcriptional activators and their release through the degradation of DELLAs in response to GA. However, the effect of GA on genome-wide expression ...
Zhang Yang Y Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, - - 2014
SIMPL (Signaling Molecule that associates with the mouse Pelle-Like Kinase) has been recently identified as a co-regulator of NF-κB dependent transcription. Here, we report the discovery and functional analysis of the SIMPL in a mollusk, Crassostrea gigas, which terms as CgSIMPL. CgSIMPL is comprised of 252 amino acids and shares ...
Du Chen C National Institutes of Health, United - - 2014
Increasing evidence indicates that transcription and alternative splicing are coordinated processes; however, our knowledge of specific factors implicated in both functions during the process of adipocyte differentiation is limited. We have previously demonstrated that the zinc finger protein ZNF638 plays a role as a transcriptional coregulator of adipocyte differentiation via ...
Narkpuk Jaraspim J Virology and Cell Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Rd, Klong 1, Klong Luang, Pathumthani 12120, - - 2014
While viral inhibition by tethering of budding virions to host cell membranes has been focused upon as one of the main functions of BST-2/tetherin, BST-2 is thought to possess other functions as well. Overexpression of BST-2 was found here to down-regulate transient protein expression. Removal of the N- and C-terminal ...
Mei Chao C MOE Systems Biology and Bioinformatics Laboratory, Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, - - 2014
A dominant suppressor of the ABAR overexpressor, soar1-1D, from CHLH/ABAR [coding for Mg-chelatase H subunit/putative abscisic acid (ABA) receptor (ABAR)] overexpression lines was screened to explore the mechanism of the ABAR-mediated ABA signalling. The SOAR1 gene encodes a pentatricopeptide repeat (PPR) protein which localizes to both the cytosol and nucleus. ...
Keung Albert J AJ Department of Biomedical Engineering and Center of Synthetic Biology, Boston University, Boston, MA 02215, USA; Howard Hughes Medical Institute, Chevy Chase, MD 20815, - - 2014
The transcription of genomic information in eukaryotes is regulated in large part by chromatin. How a diverse array of chromatin regulator (CR) proteins with different functions and genomic localization patterns coordinates chromatin activity to control transcription remains unclear. Here, we take a synthetic biology approach to decipher the complexity of ...
Fourquin Chloé C Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Valencia, Avenida de los Naranjos s/n, 46022 Valencia, - - 2014
CRABS CLAW (CRC) is a member of the YABBY family of transcription factors involved in carpel morphogenesis, floral determinacy and nectary specification in arabidopsis. CRC orthologues have been functionally characterized across angiosperms, revealing additional roles in leaf vascular development and carpel identity specification in Poaceae. These studies support an ancestral ...
Schmidt Susanne S Centre de Recherche en Biochimie Macromoléculaire; CNRS - UMR 5237; Universités Montpellier I et II; Montpellier - - 2014
Rho GTPases oscillate between an inactive GDP-bound state and an active GTP-bound state. They are activated by Rho Guanine nucleotide Exchange Factors (GEF), which accelerate the GDP to GTP exchange. RhoGEFs fall into two different classes: the Dbl family and the DOCK family of proteins. In this review, we focus ...
Mahato Simpla S Department of Biology, Indiana University, Bloomington, Indiana, United States of - - 2014
A hallmark of visual rhabdomeric photoreceptors is the expression of a rhabdomeric opsin and uniquely associated phototransduction molecules, which are incorporated into a specialized expanded apical membrane, the rhabdomere. Given the extensive utilization of rhabdomeric photoreceptors in the eyes of protostomes, here we address whether a common transcriptional mechanism exists ...
Perales Roberto R Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of - - 2014
During C. elegans development, microRNAs (miRNAs) function as molecular switches that define temporal gene expression and cell lineage patterns in a dosage-dependent manner. It is critical, therefore, that the expression of miRNAs be tightly regulated so that target mRNA expression is properly controlled. The molecular mechanisms that function to optimize ...
Espinosa L L Stem Cells and Cancer Research Laboratory, Institut Hospital del Mar d'Investigacions Mèdiques (IMIM), Barcelona, - - 2014
NF-κB pathway exerts an essential function in the regulation of the immune response, which has been the nucleus of numerous studies for the past 25 years. Both activation of the pathway and termination of the NF-κB response are tightly regulated events, which is essential to prevent exacerbated inflammatory responses. Thus, ...
Zhang Rui R Department of Colorectal Surgery, Liaoning Cancer Hospital & Institute, Shenyang, - - 2014
Background/Aims: To identify the regulator of nucleotide-binding oligomerization domain-containing protein 1 (NOD1) and its regulatory function. Methods and Results : We performed a yeast two-hybrid screening assay and identified the E3 ligase RNF34 as a candidate partner of NOD1. Using co-immunoprecipitation (co-IP) and glutathione S transferase (GST)-pull down assays, we ...
Bowers Erin E Department of Plant Pathology and Microbiology, Iowa State University , 160 Seed Science Building, Ames, Iowa 50011, United - - 2014
Field trials were conducted from 2007 to 2010 to compare grain fumonisin levels among non-Bt maize hybrids and Bt hybrids with transgenic protection against manual infestations of European corn borer (ECB) and Western bean cutworm (WBC). HPLC and ELISA were used to measure fumonisin levels. Results of the methods were ...
Garner Ashley L AL Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, 63110, - - 2014
Although the basic mechanisms of prokaryotic transcription are conserved, it has become evident that some bacteria require additional factors to allow for efficient gene transcription. CarD is an RNA polymerase (RNAP) binding protein conserved in numerous bacterial species and essential in mycobacteria. Despite the importance of CarD, its function at ...
Cho Ginam G Department of Pathology, Brigham and Women's Hospital and Harvard Medical School; Department of Pathology and Laboratory Medicine, Children's Hospital of - - 2014
Mutations in the Aristaless related homeodomain transcription factor (ARX) are associated with a diverse set of X-linked mental retardation and epilepsy syndromes in humans. Although most studies have been focused on its function in the forebrain, ARX is also expressed in other regions of the developing nervous system including the ...
Yeh Shu-Dan SD Department of Ecology and Evolutionary Biology, University of California Irvine, CA - - 2014
MicroRNAs (miRNAs) are endogenous RNA molecules that regulate gene expression post-transcriptionally. To date, the emergence of miRNAs and their patterns of sequence evolution have been analyzed in great detail. However, the extent to which miRNA expression levels have evolved over time, the role different evolutionary forces play in shaping these ...
Rønnekleiv Oline K OK Department of Physiology & Pharmacology, OR Health & Science University, Portland, OR 97239, - - 2014
The hypothalamic arcuate nucleus controls many critical homeostatic functions including energy homeostasis, reproduction and motivated behavior. Although, G-protein-coupled receptors (GPCRs) are involved in the regulation of these functions, relatively few of the GPCRs have been identified specifically within the arcuate nucleus. Here, using TaqMan low density arrays we quantified the ...
Herriges Michael J MJ Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, - - 2014
Long noncoding RNAs (lncRNAs) are thought to play important roles in regulating gene transcription, but few have well-defined expression patterns or known biological functions during mammalian development. Using a conservative pipeline to identify lncRNAs that have important biological functions, we identified 363 lncRNAs in the lung and foregut endoderm. Importantly, ...
Filloux Cyril - - 2014
Transcriptome sequencing is a powerful tool for measuring gene expression, but as well as some other technologies, various artifacts and biases affect the quantification. In order to correct some of them, several normalization approaches have emerged, differing both in the statistical strategy employed and in the type of corrected biases. ...
Bakele Martina M University of Tuebingen, - - 2014
CXCL8 (IL-8) recruits and activates neutrophils through the G protein-coupled chemokine receptor CXCR1. We showed previously that elastase cleaves CXCR1 and thereby impairs antibacterial host defense. However, the molecular intracellular machinery involved in this process remained undefined. Here we demonstrate, by using flow cytometry, confocal microscopy, subcellular fractionation, Co-IP and ...
Ghosh Sanjay S European Molecular Biology Laboratory, Heidelberg, - - 2014
In eukaryotes, RNA processing events in the nucleus influence the fate of transcripts in the cytoplasm. The multi-protein exon junction complex (EJC) associates with mRNAs concomitant with splicing in the nucleus and plays important roles in export, translation, surveillance and localization of mRNAs in the cytoplasm. In mammalian cells, the ...
Nadarajah Saralees S School of Mathematics, University of Manchester, Manchester M13 9PL, United - - 2014
Bandi et al. [Phys. Rev. E 79, 016309 (2009)] reported a closed form expression for the probability density function of the product of two correlated normal random variables and proposed an approximation for the distribution of its mean. Here, we question the closed form expression and derive exact expressions for the ...
To Kenneth Kw KK School of Pharmacy, The Chinese University of Hong Kong, Hong Kong - - 2014
This study investigated whether cholesterol levels influence the expression and function of drug transporters and whether statin treatments could alter this by reducing plasma low-density lipoprotein cholesterol levels. The mRNA expression and function of OATP1B1, ABCB1 and ABCG2 were assessed in peripheral blood mononuclear cells (PBMCs) of healthy subjects and ...
Hu Jin-Yong JY Department of Plant Breeding and Genetics, Max-Planck-Institute for Plant Breeding Research, 50829 Cologne, Germany - - 2014
The timing of flowering is pivotal for maximizing reproductive success under fluctuating environmental conditions. Flowering time is tightly controlled by complex genetic networks that integrate endogenous and exogenous cues, such as light, temperature, photoperiod, and hormones. Here, we show that AGAMOUS-LIKE16 (AGL16) and its negative regulator microRNA824 (miR824) control flowering ...
Lai Ya-Shiuan - - 2014
Whether structure and function are correlated features of organelles is a fundamental question in cell biology. Here we have queried the ability of mutants with defective structure of the endoplasmic reticulum (ER) to invoke the unfolded protein response (UPR), an essential ER signaling pathway. Through molecular and genetic approaches we ...
Fechter Pierre P Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 Rue René Descartes 67084 - - 2014
A plethora of RNAs with regulatory functions has been discovered in many non-pathogenic and pathogenic bacteria. In Staphylococcus aureus, recent findings show that a large variety of RNAs control target gene expression by diverse mechanisms and many of them are expressed in response to specific internal or external signals. These ...
Qin Yao Y State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, 61 Daizong Street, Tai'an, Shandong 271018, People's Republic of China Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of - - 2014
In this study, we carried out an evolutionary, transcriptional, and functional analyses of the trihelix transcription factor family. A total of 319 trihelix members, identified from 11 land plant species, were classified into five clades. The results of phylogeny indicate the binding domains of GT1 and GT2 diverged early in ...
Pećina-Šlaus Nives N Laboratory of Neurooncology, Croatian Institute for Brain Research, and Department of Biology, School of Medicine, University of Zagreb, Zagreb, Croatia. - - 2014
Since the discovery of the TCF/LEF family of transcription factors, their functions have been under intensive investigation in the area of cancer biology. The work presented in this paper focused on the changes in TCF-1 and LEF-1 expression levels in a set of astrocytic brain tumors. Protein expression was detected ...
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