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Sadasivam Mohanraj M Department of Biochemistry, Bharathidasan University, Tiruchirappalli, 620 024, - - 2014
Phosphoenolpyruvate carboxykinase (PEPCK) is a key gluconeogenic enzyme found in many tissues throughout the body including brain. In the present study, we have investigated the effect of bacterial lipopolysaccharide (LPS) on PEPCK and its role in neuronal steroidogenesis. Adult female albino rats were administered LPS (5mg/kg body weight) to induce ...
Savitri I J IJ Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, - - 2014
Periodontitis is an infectious disease caused by an interaction between the host and periodontopathogenic bacteria. Regulating the immune response in human gingival epithelial cells (HGEC) may contribute to the prevention of periodontitis. Irsogladine maleate (IM) has previously been shown to regulate inflammation and the cell-cell junctional barrier in HGEC. In ...
Easton Donna M DM Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Australia School of Veterinary Science, The University of Queensland, Gatton, - - 2014
Enterohaemorrhagic Escherichia coli (EHEC) are Shiga toxigenic pathogens capable of inducing severe forms of enteritis (e.g. hemorrhagic colitis) and extraintestinal sequelae (e.g. hemolytic uremic syndrome). The molecular basis of colonization of human and animal hosts by EHEC is not yet completely understood and an improved understanding of EHEC mucosal adherence ...
Wei Zhengkai Z College of Veterinary Medicine, Jilin University, Changchun, 130062, Jilin Province, - - 2014
Selenium, in the form of selenoproteins, plays a pivotal role in anti-inflammatory processes and antioxidant defense system. The aim of this study was to examine the effects of selenium on lipopolysaccharide (LPS)-induced inflammatory responses in bovine mammary epithelial cells (bMEC) and to investigate the potential mechanism. bMEC viability was measured ...
Wu Hong H MOE Key Lab of Bioinformatics, Department of Biological Science and Biotechnology, School of Life Science, Tsinghua-Peking Center for Life Sciences, Tsinghua University, 100084, Beijing, - - 2014
Micro-aeration is a situation that will be encountered in bacterial cell growth especially when the saturated dissolved oxygen level cannot match the demand from cells grown to a high density. Therefore, it is desirable to separate aerobic growth and micro-aerobic product formation into two stages using methods including anaerobic or ...
Zimmermann Katrin K Friedrich-Loeffler-Institut, Institute of Animal Welfare and Animal Husbandry, Celle, - - 2014
Serotonin (5-hydroxytryptamine; 5-HT) transporter (5-HTT) is involved in inflammation and the stress response. In this study, we examined the regulation of 5-HTT expression in macrophage HD11 cells in response to bacterial LPS. Long-term exposure of cells to LPS (6-18 h) produced a decrease in 5-HTT mRNA expression. Accordingly, reduced 5-HTT activity ...
Gómez-Lozano María - - 2014
RNA sequencing technologies reveal that bacteria express RNA molecules other than mRNA, rRNA or tRNA. During the last years genome-wide bacterial transcriptomes have been shown to comprise intergenic RNA, antisense RNA, and untranslated regions, all capable of performing diverse regulatory functions. In this study we used RNA-seq to identify 232 ...
Fu E E Department of Periodontology, National Defense Medical Center, Research Institute of Dental Sciences, School of Dentistry, Tri-Service General Hospital, Taipei, - - 2014
Diallyl sulfide (DAS), a flavor compound from garlic, has varied potential therapeutic activities. Periodontitis is a disease that develops because of host-mediated inflammation to periodontal pathogens. In this study, the effects of DAS on the common proinflammatory cytokines and nuclear factor-kappa B (NF-κB) in human gingival fibroblasts (HGFs) being stimulated ...
Murata H H Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, - - 2014
Tumor necrosis factor alpha (TNF-α) is a major cytokine implicated in various inflammatory diseases. The nature of the nuclear factors associated with human TNF-α gene regulation is not well elucidated. We previously identified a novel region located from -550 to -487 in human TNF-α promoter that did not contain the ...
Metsola Juhani J Department of Pediatrics, Institute of Clinical Medicine and Medical Research Center, Oulu University Hospital and University of Oulu, Oulu, - - 2014
Background:Adenosine levels are regulated by ecto-5'-nucleotidase/CD73 and adenosine deaminase (ADA). Adenosine regulates endothelial permeability and anti-inflammatory responses via adenosine receptors. Here, the adenosine receptors and purine-converting enzymes were studied during postnatal development and inflammation.Methods:Newborn, 1-, 10-, 14-day-old and adult C57BL/6 mice were challenged i.p. with LPS for six hours. The ...
Yu Liming - - 2014
Chronic inflammation underscores the pathogenesis of a range of human diseases. Lipopolysaccharide (LPS) elicits strong pro-inflammatory response in macrophages via the transcription factor NF-κB. The epigenetic mechanism underlying LPS-induced pro-inflammatory transcription is not completely appreciated. Herein we describe a role for myocardin related transcription factor A, or MRTF-A, in this ...
Vissers Marloes M Laboratory of Pediatric Infectious Diseases, Department of Pediatrics, Radboud university medical center, Nijmegen, The Netherlands Laboratory of Medical Immunology, Department of Laboratory Medicine, Radboud university medical center, Nijmegen, The - - 2014
Matrix metallopeptidase-9 (MMP-9) is a protease involved in the degradation of extracellular matrix collagen. Evidence suggests that MMP-9 is involved in the pathogenesis during a Streptococcus pneumoniae infection. However, not much is known about the induction of MMP-9 and the regulatory processes involved. We show that the Gram-positive bacteria used ...
Eslani Medi M Ophthalmology and Visual Sciences, University of Illinois at Chicago, 1855 W. Taylor Street EEI, 3164, Chicago, Illinois, 60660, United - - 2014
Purpose: To evaluate the role of TLR4 in corneal epithelial wound healing. Methods: The expression of TLR4 during in vivo corneal epithelial wound healing was examined by immunostaining in mice. The expression and activation of TLR4 was studied in primary or telomerase-immortalized human corneal epithelial cells (HCEC). Scratch assay was ...
Yi Nan N Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. - - 2014
Peroxiredoxin 4 (Prx4) has a number of important biological functions, such as efficient antioxidant capacity and promotion of cell proliferation and differentiation. The purpose of this study was to investigate the expression and significance of Prx4 in human colorectal cancer (CRC). Quantitative polymerase chain reaction (qPCR) was performed to detect ...
Wu Jie J Department of Oncology, The First Affiliated Hospital of Liaoning Medical University, Liaoning, Jinzhou 121000, P.R. - - 2014
Wiskott-Aldrich syndrome protein family member 3 (WASF3) is required for invasion and metastasis in different cancer cell types, and has been demonstrated to possess prognostic value in various types of human cancer. However, to the best of our knowledge, the expression profile of WASF3 and its correlations with the clinicopathological ...
Gao Chaobing C Department of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. - - 2014
Artemin (ARTN) has been implicated in the development and progression of several human malignancies. However, the clinical and prognostic significance of ARTN and its receptors has not yet been investigated in human laryngeal squamous cell carcinoma (LSCC). Therefore, in the present study, the protein expression of ARTN and its receptor, ...
Lawrence H H Newcastle University, Institute of Cellular Medicine, 3rd Floor, William Leech Building, Faculty of Medical Sciences, Framlington Place, Newcastle upon Tyne, NE2 4HH, - - 2014
Abnormal wear of cobalt-containing metal-on-metal joints is associated with inflammatory pseudotumours. Cobalt ions activate human toll-like receptor 4 (TLR4), which normally responds to bacterial lipopolysaccharide (LPS) in sepsis. Activation of TLR4 by LPS increases the expression of chemokines IL-8 and CXCL10, which recruit leukocytes and activated T-cells, respectively. This study ...
Morris Antrison A Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio state University, Wooster, OH 44691, United States of - - 2014
25-hydroxycholecalciferol (25(OH)D) is an intermediate metabolite during the biosynthesis of active vitamin D. In vitro studies were conducted in chicken monocytes and a chicken macrophage cell line (HD11) to study the effects of supplementing with 25(OH)D on nitrite production and mRNA amounts of interleukin (IL)-1β, IL-10, 1α-hydroxylase and 24-hydroxylase post- ...
Fu Jianfei J Institute of Preventative Medicine and Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, School of Medicine, Ningbo University, Ningbo, Zhejiang, - - 2014
Adipose tissue is functionally composed of brown adipose tissue (BAT) and white adipose tissue (WAT). The unique thermogenic capacity of BAT results from expression of uncoupling protein 1 (UCP1) in the mitochondrial inner membrane. Based on recent findings that adult humans have functionally active BAT, BAT is now recognized as ...
Ackermann M M Department of Neurosurgery, University Clinic of Regensburg, Regensburg, - - 2014
Hydrogen sulfide (H2S) is the first inorganic compound identified as both a substrate for mitochondrial oxidative phosphorylation and a transmitter in mammalian cells. H2S seems to mediate effects that are correlated with those of nitric oxide (NO) by a reciprocal regulation. Moreover, H2S is consumed by mitochondrial oxidation mediated by ...
Wang Huidong H Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, PR - - 2014
RNA interference (RNAi) signal can spread from the point where the double-stranded RNA (dsRNA) was initially applied to other cells or tissues. SID-related genes in Caenorhabditis elegans help in the spreading of this signal. However, the mechanisms of systemic RNAi are still not unveiled in insects. In this study, we ...
Kuwasako Kanako K 1] RIKEN Systems and Structural Biology Center, Yokohama, Japan. [2] RIKEN Center for Life Science Technologies, Yokohama, Japan. [3] Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Nishitokyo, Japan. - - 2014
Tissue-specific alternative pre-mRNA splicing is often cooperatively regulated by multiple splicing factors, but the structural basis of cooperative RNA recognition is poorly understood. In Caenorhabditis elegans, ligand binding specificity of fibroblast growth factor receptors (FGFRs) is determined by mutually exclusive alternative splicing of the sole FGFR gene, egl-15. Here we ...
Xiao Ning N Department of Urology, The Affiliated Hospital of Guilin Medical College, Guilin, People's Republic of - - 2014
Transient receptor protein (TRP) channels are frequently associated with tumors and are correlated to patient's outcome. We firstly investigated TRP channel melastatin 8 (TRPM8) expression in urothelial carcinoma of bladder (UCB) and its correlation with UCB clinicopathological features and additionally evaluated the association between TRPM8 expression and patients' survival rate ...
Marrero-Rodríguez Daniel D Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, CMN-SXXI. IMSS, Av. Cuauhtémoc 330, Col. Doctores, México, D.F., 06720, - - 2014
Cervical cancer (CC) as other cancer types, presents molecular deregulations, such as the alterations of transcription factors. Krüppel-like factors (KLF) are a family of transcriptional regulators. They are involved in diverse cellular processes, such as proliferation, apoptosis, and angiogenesis among others. Here, we analyzed the expression of all 17 KLF ...
Catalán Victoria V Metabolic Research Laboratory, Clínica Universidad de Navarra, Pamplona, - - 2014
The ribosomal protein S6 kinase 1 (S6K1) is a component of the insulin signalling pathway that has been proposed as a key molecular factor in insulin resistance development under conditions of nutrient overload. The aim was to evaluate the involvement of S6K1 in obesity as well as to explore their ...
Monteiro César B CB Departamento de Biologia Experimental, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal; Morfofisiologia do Sistema Somatosensitivo, IBMC - Instituto de Biologia Celular e Molecular, 4150-180 Porto, Portugal. Electronic address: - - 2014
The homeodomain factor Prrxl1 is crucial for proper assembly of dorsal root ganglia (DRG)-dorsal spinal cord (SC) pain-sensing circuit. By performing chromatin immunoprecipitation with either embryonic DRG or dorsal SC, we identified two evolutionarily conserved regions (i.e. proximal promoter and intron 4) of Prrxl1 locus that show tissue-specific binding of ...
Odkhuu Erdenezaya E Department of Microbiology and Immunology, Aichi Medical University School of Medicine, Nagakute, Aichi 480-1195, Japan; Departments of Anatomy, Mongolian National University of Medical Sciences, Ulaanbaatar 210648, Mongolia. Electronic address: - - 2014
The effect of lipopolysaccharide (LPS) on the expression of p53 protein in RAW 264.7 macrophage cells was examined. LPS downregulated the expression of p53 protein 4-24h after the stimulation. LPS-induced p53 inhibition was restored with pharmacological inhibitors of c-jun N-terminal kinase (JNK) and phosphatidylinositol 3-kinase (PI3K). It was also restored ...
Schmitt Bianca M BM University of - - 2014
The genetic code is an abstraction of how mRNA codons and tRNA anticodons molecularly interact during protein synthesis; the stability and regulation of this interaction remains largely unexplored. Here, we characterized the expression of mRNA and tRNA genes quantitatively at multiple time points in two developing mouse tissues. We discovered ...
Fu Yan Y Department of Oncology, The First Affiliated Hospital, Chinese PLA General Hospital, No. 51, Fucheng Road, Beijing, 100048, - - 2014
Hepatocellular carcinoma (HCC) remains one of the most common cancers worldwide. HSPA2 has been highlighted as an important marker in many types of cancers. However, little is known about the role of HSPA2 in HCC. The objective of the current study was to investigate the expression pattern and clinicopathological significance ...
Ni Meng M Fisheries College, Ocean University of China, 5 Yushan Road, Qingdao, Shandong, 266003, People's Republic of - - 2014
In this study, the cDNA sequences of HSP70 and HSP90 were isolated from the special chondr-ganoid scale, Amur sturgeon, for the first time. Homology analysis indicated that amino acid sequences of HSP70 and HSP90 shared high identity with other species (82.68-99.07 and 90.19-98.07 %, respectively). The tissue expression analysis showed that ...
He Z Z School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, P. R. China; College of Animal Sciences and Technology, Sichuan Agricultural University, Ya'an 625014, Sichuan, P. R. - - 2014
The ovarian differentiation, morphogenesis and expression of some putative gonadal development-related genes were analysed in the ricefield eel Monopterus albus, a protogynous hermaphroditic teleost with a single elongate ovary. At c. 1 day post-hatching (dph), the gonadal ridge was colonized with primordial germ cells (PGCs) at the periphery and transformed ...
Liu Lixing L Department of Anatomy, Fujian Medical University, Fuzhou, - - 2014
Aim: To investigate the expression and clinical significance of ATP-binding cassette transporter 1 (ABCA1) in pregnant women with preeclampsia (PE). Methods: 52 pregnant women with PE who were admitted for delivery were enrolled in the study, while 30 normal pregnant inpatients were chosen as controls. Blood lipid and serum ABCA1 ...
Huang Qi Q Department of Respiratory and Critical Care Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, 430022, Hubei, People's Republic of - - 2014
Interleukin-17 receptor (IL-17RA) is essential for proinflammatory cytokine IL-17-mediated pathogenesis of various tumors. IL-17RA is upregulated by some proinflammatory cytokines such as IL-21 and IL-15 and downregulated by IL-2, while the effect of IL-1β, IL-6, IL-8, TNF-α on IL-17RA expression in non-small cell lung caner (NSCLC) remains unknown. Our findings ...
Bhat Imtiyaz A IA Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, 190011, India, - - 2014
A new class of compounds targeting cyclooxygenase 2 (COX-2) together with other different clinically used therapeutic strategies has recently shown a promise for the chemoprevention of several solid tumors including lung cancer. The aim was to study the possible role of COX-2 -8473 T/C NP and its expression in the ...
Fossati Sara Maria SM Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genoa, Italy, - - 2014
Acetylcholinesterase (ACHE) is a glycoprotein with a key role in terminating synaptic transmission in cholinergic neurons of both vertebrates and invertebrates. ACHE is also involved in the regulation of cell growth and morphogenesis during embryogenesis and regeneration acting through its non-cholinergic sites. The mollusk Octopus vulgaris provides a powerful model ...
Jeong Woo-Jin WJ http://orcid.org/0000-0001-8577-6964 Department of Otorhinolaryngology-Head & Neck Surgery, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam, South - - 2014
Perineural invasion and expression of CXCR4 is characteristic of adenoid cystic carcinoma (ACC). Herein, we aimed to demonstrate CXCR4 expression in ACC, identify its association with perineural invasion and investigate the impact of CXCR4 inhibitor in vitro and in a murine perineural invasion model. Expression of CXCR4 was assessed in ...
van Zeijl Clementine Jj CJ Academic Medical - - 2014
Thyrostimulin, a putative glycoprotein hormone, comprises of the subunits GPA2 and GPB5 and activates the TSH receptor (TSHR). The observation that proinflammatory cytokines stimulate GPB5 transcription suggested a role for thyrostimulin in the pathogenesis of nonthyroidal illness (NTIS). In the present study, we induced acute inflammation by lipopolysaccharide (LPS) administration ...
Takahashi Toshiaki T National Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin, - - 2014
Introduction Malformation of the nonmuscular tissue components in congenital diaphragmatic hernia (CDH) is thought to underlie the diaphragmatic defect, causing intrathoracic herniation of abdominal viscera and thus disturbing normal lung development. It has been shown that diaphragmatic and pulmonary morphogeneses require the structural integrity of connective tissue, and developmental mutations that ...
Bao Jia-Peng JP Department of Orthopedics Surgery, The Second Hospital of Medical College, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. - - 2014
Visceral adipose tissue‑derived serine protease inhibitor (vaspin) is a newly identified member of the adipocytokine family, whose precise role in chondrocyte metabolism remains to be elucidated. The aim of the present study was to investigate the effect of vaspin on chondrocytes. The cell viability and the cytotoxicity of vaspin in ...
Yang Tao T Department of Hepatobiliary Surgery, The First Hospital of Shijiazhuang City, Shijiazhuang, - - 2014
Sox17, an antagonist of canonical Wnt/β-catenin signaling, inhibits several malignant carcinogenesis and progression. However, little is known about Sox17 in hepatocellular carcinoma (HCC). Here, we found that Sox17 is downregulated in HCC tissue. Furthermore, Sox17 inhibits cell proliferation and migration in HCC. KIF14, a member of kinesin superfamily protein (KIFs), ...
Wang Lei L Institute of Laboratory Medicine, Hubei University of Chinese Medicine, Huangjia Lake West Road, Wuhan, 430065, Hubei Province, China, - - 2014
Research has shown the essential role of interleukin-33 (IL-33) in driving protective anti-viral immunity. IFN-γ has been reported to improve IL-33 expression in cultured epithelial cells. The development of cervical intraepithelial neoplasia (CIN) and carcinogenesis was closely related to human papilloma virus (HPV) infection and defective anti-viral immunity. The aim ...
Wang Xiaohong X Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. - - 2014
Pelvic organ prolapse (POP) is a common disorder that can disturb the health and quality of life of females. However, the basic pathophysiology and underlying mechanism of POP are not fully understood. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) have been reported to be associated with the onset ...
Chen Shao-Qin SQ Shao-Qin Chen, Zhi-Hua Chen, Su-Yong Lin, Qi-Bao Dai, Department of Gastrointestinal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian Province, - - 2014
To examine the effect of aberrant methylation of the KISS1 promoter on the development of colorectal cancer (CRC) and to investigate reversing aberrant methylation of the KISS1 promoter as a potential therapeutic target. KISS1 promoter methylation, mRNA expression and protein expression were detected by methylation-specific polymerase chain reaction (PCR), real-time ...
Mathew Esha E University of Michigan, United - - 2014
Pancreatic cancer, one of the deadliest human malignancies, is almost uniformly associated with a mutant, constitutively active form of the oncogene Kras. Studies in genetically engineered mouse models have defined a requirement for oncogenic KRAS in both the formation of PanINs -the most common precursor lesions to pancreatic cancer- and ...
Rajakumar Anbazhagan A Laboratory of Molecular Endocrinology and Reproductive Biology, Department of Animal Sciences, School of Life Sciences-Centre for Advance Studies, University of Hyderabad, P.O. Central University, Hyderabad 500046, - - 2014
In teleosts, the levels of steroids are critical for sexual development and hence, expression of steroidogenic enzyme genes and specific substrate availability are indispensable for gonadal steroidogenesis. Early stages of steroidogenesis specifically cholesterol to pregnenolone conversion by Cyp11a1 is crucial for estradiol and testosterone biosynthesis. Based on this, in this ...
Lipskaia Larissa L Mount Sinai School of Medicine, Cardiovascular Research Center, NY, USA; Inserm, U955, Equipe 8, Créteil, France; Université Paris-Est, Faculté de médecine, Créteil, - - 2014
The sarco(endo)plasmic reticulum Ca(2+) ATPases (SERCA) system, a key regulator of calcium cycling and signaling, is composed of several isoforms. We aimed to characterize the expression of SERCA isoforms in mouse cardiovascular tissues and their modulation in cardiovascular pathologies (heart failure and/or atherosclerosis). Five isoforms (SERCA2a, 2b, 3a, 3b and ...
Bell Glenn D GD Department of Molecular Medicine & Pathology, Faculty of Medical and Health Sciences, University of Auckland, Auckland 1005, New - - 2014
Here we sought evidence for the existence of insulin mRNA-producing cells outside the human pancreas. Commercially available complementary DNA (cDNA) arrays prepared from 72 different types of adult human tissues were screened by PCR for transcripts encoding insulin, and other classic pancreatic hormones. Insulin mRNA transcripts were detected by standard ...
Zhang Luan-Jing - - 2014
Golgi phosphoprotein 3 (GOLPH3) has been identified as an oncoprotein in various human cancers; however, its role in pancreatic ductal adenocarcinoma (PDAC) is unknown. We examined GOLPH3 expression levels and relationship with survival in patients with PDAC to establish the significance of GOLPH3 in the development and progression of PDAC. ...
Kanda Mitsuro - - 2014
BackgroundGastric cancer (GC) remains to have a poor prognosis via diverse process of cancer progression. Dihydropyrimidinase-like 3 (DPYSL3) is a cell adhesion molecule that has been reported to be involved in the metastatic process of tumor cells. The aim of this study was to identify a novel clinically-relevant biomarker of ...
Shao Ning N Department of Urology, Second People's Hospital of Wuxi Affiliated to Nanjing Medical University, 68 Zhongshan Road, Wuxi, 214002, - - 2014
Several genes encoding DNA repair molecules have been proposed as cancer-susceptibility genes. Many studies have suggested that SNPs in XRCC4 could be implicated in altering the risk of prostate cancer (PCa). We examined the role of the functional variant (-652T>G) in the XRCC4 promoter in PCa. The transcriptional activity of ...
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