Search Results
Results 1 - 50 of 1646
1 2 3 4 5 6 7 8 9 10 >
Vincelli Gabriele G Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Via Irnerio 48, 40126, Bologna, (BO), - - 2015
The regulation of MOR expression at the level of mRNA is relevant for its role in pain transmission and in other functions involving opioid receptors. Recently, the role of the 3'UTR in the posttranscriptional regulation of MOR expression has been highlighted. Here we describe a Renilla luciferase reporter assay for ...
Yin Kaifeng - - 2014
In the rodent incisor during amelogenesis, as ameloblast cells transition from secretory stage to maturation stage, their morphology and transcriptome profiles change dramatically. Prior whole genome transcriptome analysis has given a broad picture of the molecular activities dominating both stages of amelogenesis, but this type of analysis has not included ...
Zhan Jun-Kun - - 2014
BackgroundArterial calcification is an important pathological change of diabetic vascular complication. Osteoblastic differentiation of vascular smooth muscle cells (VSMCs) plays an important cytopathologic role in arterial calcification. The glucagon-like peptide-1 receptor agonists (GLP-1RA), a novel type of antidiabetic drugs, exert cardioprotective effects through the GLP-1 receptor (GLP-1R). However, the question ...
Islam Md Nurul MN Department of Orthodontics, Science of Functional Recovery and Reconstruction, Okayama University Graduate School of Medicine, , Dentistry and Pharmaceutical Sciences, Okayama, - - 2014
Background: The rodent salivary gland is not fully developed at birth and the cellular definitive differentiation takes place postnatally. However, little is known about its molecular mechanism. Results: Here we provide the loss-of-function genetic evidence that Runx signaling affects postnatal development of the submandibular gland (SMG). Core binding factor β ...
Wang Yingfeng Y State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Diseases, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang Road, Guangzhou, Guangdong, 510120, People's Republic of - - 2014
In this study, we investigated the role of peroxisome proliferator-activated receptor γ (PPARγ) on store-operated calcium entry (SOCE) and expression of the main store-operated calcium channel (SOCCs) components, canonical transient receptor potential (TRPC) in chronic hypoxia (CH)-induced pulmonary hypertension (CHPH) rat models. Small interfering RNA (siRNA) knockdown and adenoviral overexpression ...
Park Gyeong-Hun GH 1 Department of Dermatology, Dongtan Sacred Heart Hospital, Hallym University College of Medicine , Hwaseong, - - 2014
Abstract Ginseng has been shown to promote hair growth in several recent studies. However, its effects on human hair follicles and its mechanisms of action have not been sufficiently elucidated. This study aimed to investigate the hair growth-promoting effects of red ginseng extract (RGE) and its ginsenosides. The proliferative activities ...
Xin Xiao-Min XM Xiao-Min Xin, Mu-Xiao Zhong, Gong-Li Yang, Yao Peng, Ya-Li Zhang, Wei Zhu, Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, - - 2014
To investigate the effect of GW4064 on the expression of adipokines and their receptors during differentiation of 3T3-L1 preadipocytes and in HepG2 cells. The mRNA expression of farnesoid X receptor (FXR), peroxisome proliferator-activated receptor-gamma 2 (PPAR-γ2), adiponectin, leptin, resistin, adiponectin receptor 1 (AdipoR1), adiponectin receptor 2 (AdipoR2), and the long ...
Rochman M M Divisions of Allergy and Immunology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio, - - 2014
Although interleukin (IL)-13 and neurotrophins are functionally important for the pathogenesis of immune responses, the interaction of these pathways has not been explored. Herein, by interrogating IL-13-induced responses in human epithelial cells we show that neurotrophic tyrosine kinase receptor, type 1 (NTRK1), a cognate, high-affinity receptor for nerve growth factor ...
Zemirli Naima - - 2014
BackgroundThe nuclear factor ¿B (NF-¿B) family members regulate several biological processes as cell proliferation and differentiation, inflammation, immunity and tumor progression. Ubiquitination plays a key role in NF-¿B activation and the ubiquitylated transmitters of the NF-¿B signaling cascade accumulate in close proximity to endomembranes.FindingsWe performed an unbiased siRNA library screen ...
Englert Neal A NA NIEHS, United - - 2014
The Mediator complex is vital for the transcriptional regulation of eukaryotic genes. Mediator binds to nuclear receptors at target response elements and recruits chromatin modifying enzymes and RNA Pol II. Here, we examine the involvement of Mediator subunit MED25 in the epigenetic regulation of human cytochrome P450 2C9 (CYP2C9)(2). MED25 ...
Gao Mengsheng M Soil and Water Science Department, Genetics Institute, University of Florida - Institute of Food and Agricultural - - 2014
In Sinorhizobium meliloti, timing of Quorum Sensing-dependent gene expression is controlled at multiple levels. RNA binding protein Hfq contributes to the regulation of QS signal production, and this regulation is exerted both in the manner that involves AHL receptor ExpR, and via expR-independent mechanisms. In the expR+ strain of S. ...
El-Hayek Stephany S Departments of Obstetrics and Gynecology, Biology, and Research Institute-McGill University Health Centre; Montreal, QC, Canada H3A 1A1; - - 2014
Fertility depends on the precise coordination of multiple events within the ovarian follicle to ensure ovulation of a fertilizable egg. FSH promotes late follicular development, including expression of luteinizing hormone (LH) receptor by the granulosa cells. Expression of its receptor permits the subsequent LH surge to trigger the release of ...
Arai Iwao I Department of Pharmacology, International University of Health and Welfare, 2600-1, Kitakanemaru, Ohtawara, Tochigi, 324-8510, Japan; Department of Molecular Immunology, Institute of DNA medicine, Jikei University, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105-8461, - - 2014
We investigated the effects of repeated administration of interleukin-31 (IL-31) on itch-associated scratching counts (long-lasting scratching, LLS) and IL-31-related receptor mRNA expression in mice. Intradermal (i.d.) injection of IL-31 (100 and 300 ng/site) every 12 h for 3 days significantly increased LLS. Repeated administration of IL-31 also increased the expression ...
Kobayashi Yusuke Y Department of Obstetrics and Gynecology, Nihon University School of Medicine, Tokyo, - - 2014
This study investigated whether angiotensin II type 1 receptor agonistic autoantibodies (AT1 -AAs) mediate the increased release of soluble endoglin (sEng) in women with preeclampsia. Serum samples were obtained from women with normal pregnancies or with preeclampsia. Human first-trimester trophoblast cells were cultured with purified IgG derived from these sera, ...
Freeman Stephen D SD Laboratory for Sensory Development, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe-shi, - - 2014
Background: Inner ear morphogenesis is tightly regulated by the temporally and spatially coordinated action of signaling ligands and their receptors. Ligand - receptor interactions are influenced by heparan sulfate proteoglycans (HSPGs), cell surface molecules that consist of glycosaminoglycan chains bound to a protein core. Diversity in the sulfation pattern within ...
Skipor Janina J Polish Academy of Sciences Institute of Animal Reproduction and Food Research ul. Tuwima 10 10-748 Olsztyn - - 2014
The blood-cerebrospinal fluid barrier (BCSFB) located in the epithelial cells of the choroid plexus (CP) forms the interface between the cerebrospinal fluid (CSF) and pathogen components circulating in the blood. The CP is also implicated in the passage of peripheral immune signals and circulation of immune cells into the central ...
Ananthanarayanan Meenakshisundaram M Yale University, United States; - - 2014
The type III isoform of the inositol 1,4,5-trisphosphate receptor (InsP3R3) is apically localized and triggers Ca2+ waves and secretion in a number of polarized epithelia. However, nothing is known about epigenetic regulation of this InsP3R isoform. We investigated miRNA regulation of InsP3R3 in primary bile duct epithelia (cholangiocytes) and in ...
Myllärniemi Marjukka - - 2014
Activins are members of the TGF-Ss superfamily of growth factors. First, we identified by expression array screening that activin-B and follistatin are upregulated in human idiopathic pulmonary fibrosis (IPF). Next, we wanted to clarify their specific role in lung fibrosis formation. We used specific antibodies for activin-A and -B subunits ...
Jiang Ling L Department of Pediatrics, The First Affiliated Hospital of GuangXi Medical University , Nanning , China - - 2014
Abstract Peroxisome proliferator-activated receptorγ (PPARγ) can regulate the process of cell apoptosis and is related to the progression of renal disorders. Retinoic acid receptor alpha (RARα) is one of the nuclear receptors involved in a variety of kidney diseases. Renal interstitial fibrosis (RIF) is a common denominator of chronic kidney ...
Song William W C WW Sydney Medical School and Charles Perkins Centre, University of Sydney, Sydney, - - 2014
Fat cell differentiation (FCD) potentiates adipose cell characteristics including lipid storage and insulin sensitivity. In vitro, we have demonstrated that CCN2, also known as connective tissue growth factor (CTGF), inhibits FCD in NIH3T3-L1 cells and in adipocytes isolated from mouse epididymal fat pads. The aim of this study was to ...
Xu Changxin C Department of Oncological Sciences and Medicine, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT, - - 2014
We previously found that conditional deletion of integrin β1 in intestinal epithelium of mice caused early postnatal lethality and intestinal phenotypic changes including excessive proliferation and defective differentiation of intestinal epithelium due to loss of Hedgehog expression. Here, we link these defects to the Hedgehog (Hh) signaling pathway and show ...
Delgadin Tomás Horacio TH Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EGA, Buenos Aires, - - 2014
Cichlasoma dimerus is a social cichlid fish capable of growing at high rates under laboratory conditions, but knowledge on somatic growth regulation is still unclear. Growth hormone (GH)/insulin-like growth factor I (IGF-I) axis is the key regulator of somatic growth in vertebrates. Two types of growth hormone receptors have been ...
Parua Pabitra K PK University of Washington, United - - 2014
In eukaryotes combinatorial activation of transcription is an important component of gene regulation. In the budding yeast Saccharomyces cerevisiae, Adr1-Cat8 and Adr1-Oaf1/Pip2 are pairs of activators that act together to regulate two diverse sets of genes. Transcription activation of both sets is regulated positively by the yeast AMPK homologue, Snf1 ...
Wi Sae Mi SM Department of Molecular Cell Biology and Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of - - 2014
Recent evidence shows that evolutionarily conserved signaling intermediate in Toll pathways (ECSIT) interacts with tumor necrosis factor receptor-associated factor 6 (TRAF6) ubiquitinated, and contributes to bactericidal activity during Toll-like receptor (TLR) signaling. Here, we first report a new regulatory role for ECSIT in TLR4 signaling. On TLR4 stimulation, endogenous ECSIT ...
Yan Yan Y Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, Kurume, Fukuoka, 830-0011, Japan; Department of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100730, - - 2014
Peroxisome proliferator-activated receptors (PPARs) are potentially useful for treatment of skin diseases, because they stimulate keratinocyte differentiation, exert anti-inflammatory effects and improve barrier function. We examined five PPAR-γ agonists, including four thiazolidinediones (ciglitazone, troglitazone, rosiglitazone and pioglitazone) and an angiotensin-II receptor blocker (telmisartan), for their ability to up-regulate filaggrin and ...
Chen Xu X Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, P.R. - - 2014
Increasing evidence indicates that transforming growth factor-β1 (TGF-β1) is a pivotal mediator in the pathogenesis of renal fibrosis. Mesangial cells (MCs) are important for glomerular function under both physiological and pathological conditions. Studies have found that the expression level of prostaglandin E2 (PGE2) in MCs increases under high glucose conditions, that ...
Rovani Monique - - 2014
BackgroundEstradiol (E2) receptors mediate E2 effects on cell proliferation and apoptosis under normal and pathological conditions. However, the mechanisms involved in E2 signaling are not completely understood. The objectives in this study were to evaluate the expression of estrogen receptors (ESRs) during follicular selection in cattle, and the effect of ...
Sabag Adi D - - 2014
Class-3 semaphorins are anti-angiogenic and anti-tumorigenic guidance factors that bind to neuropilins which in turn associate with class-A plexins to transduce semaphorin signals. To study the role of the plexin-A2 receptor in semaphorin signaling, we silenced its expression in endothelial cells and in glioblastoma cells. The silencing did not affect ...
Arantes Ricardo Vinicius Nunes RV University of São Paulo, Bauru School of Dentistry, Department of Biological Sciences, Bauru, São Paulo, - - 2014
Background: Vascular endothelial growth factor (VEGF) plays an important role during angiogenesis and bone repair. This study investigated whether the use of meloxicam alters bone repair via down-regulation of VEGF and its receptors expression. Methods: 120 male Wistar rats had their upper right incisor extracted. Animals were divided into control ...
Kurakula Kondababu K Department of Medical - - 2014
The LIM-only protein FHL2 is expressed in smooth muscle cells (SMCs) and inhibits SMC-rich lesion formation. To further elucidate the role of FHL2 in SMCs we compared the transcriptome of SMCs derived from wild-type (WT) and FHL2-knockout (KO) mice. This revealed that in addition to previously recognized involvement of FHL2 ...
Zhou Zhen-Xing ZX Institute of Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, - - 2014
Quorum sensing molecular γ-butyrolactones (GBL) are widely distributed among the genus Streptomyces. Their cognate receptors have been demonstrated to control secondary metabolism and/or morphological differentiation. ScgA is responsible for the biosynthesis of GBL in Streptomyces chattanoogensis. According to the genome-wide transcriptome analysis of the ΔscgA mutant, we found that the ...
Jacob Aude A Inserm, UMR-S 1144, Paris, F-75006, France; Université Paris Descartes, UMR-S 1144, Paris, F-75006, France; Université Paris Diderot, UMR-S 1144, Paris, F-75013, - - 2014
The expression of aryl hydrocarbon receptor (AhR) transcription factor was detected at transcript level in freshly isolated human brain microvessels and in the hCMEC/D3 human cerebral microvascular endothelial cell line. Recent studies have demonstrated that AhR pathway is able to crosstalk with other pathways such as hypoxia signaling pathway. Therefore, ...
Lee D D Department of Biology, University of Toronto Mississauga, Mississauga, ON, - - 2014
Myosuppressins are a family of the FMRFamide-like peptides. They have been characterized in many insects and shown to inhibit visceral muscle contraction. Rhodnius prolixus possesses an unusual myosuppressin in that the typical FLRFamide C-terminal motif is unique and ends with FMRFamide. In the present study, we isolated the cDNA sequence ...
Kempisty Bartosz B Department of Histology and Embryology,Department of Anatomy,PoznanUniversity of Medical Sciences,6 Swiecickiego St.,60-781 - - 2014
Summary Although the expression of estrogen and progesterone receptors within porcine ovary and cumulus-oocyte complexes (COCs) is well recognized, still little information is known regarding expression of the progesterone receptor (PGR), PGR membrane component 1 (PGRMC1) and of estrogen-related receptors (ERRγ and ERRβ/γ) in separated cumulus cells in relation to ...
Seth-Vollenweider Tanya T Rutgers- New Jersey Medical School, United - - 2014
The SWI/SNF chromatin remodeling complex facilitates gene transcription by remodeling chromatin using the energy of ATP hydrolysis. Recent studies have indicated an interplay between the SWI/SNF complex and protein arginine methyltransferases (PRMTs). Little is known however about the role of SWI/SNF and PRMTs in vitamin D receptor (VDR) mediated transcription. ...
Peng Bo B Department of Obstetrics & Gynecology, Child & Family Research Institute, University of British Columbia, Vancouver, BC, - - 2014
Gonadotropin Releasing Hormone (GnRH) and its receptor GnRHR were shown to promote trophoblastic cell invasion. Detection of elevated early development related transcription factor TWIST and adhesion molecule N-cadherin in the invasive trophoblastic cells could possible suggest that GnRH promotes trophoblastic cell invasion through TWIST regulated N-cadherin pathway. To investigate the ...
Tabandeh Mohammad Reza MR Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz , Ahvaz , Iran - - 2014
Abstract Context: Rb1 ginsenoside, the key element of ginseng root, is widely used as an herbal therapeutic drug in diabetic patients. Various hypoglycemic mechanisms have been described for Rb1; however, to date, there has been no report on the effect of Rb1 on adiponectin signaling. Objectives: The current study was ...
Hong Hua H Department of Molecular Medicine, College of Medicine, Keimyung University, Dalseo-gu, Daegu 704-701, Republic of - - 2014
Hearing loss can be induced by multiple causes, including cochlear inflammation. Prednisone (PDN) is a well‑known steroid clinically used in the treatment of hearing loss. In the present study, we investigated the inhibitory effects and the mechanisms of action of PDN on the expression of cyclooxygenase (COX)-2, an inflammatory enzyme ...
Kühn Lukas C LC Ecole Polytechnique Fédérale de Lausanne (EPFL), ISREC - Swiss Institute for Experimental Cancer Research, EPFL_SV_ISREC, Room SV2516, Station 19, CH-1015 Lausanne, Switzerland. - - 2014
Cellular iron homeostasis is regulated by post-transcriptional feedback mechanisms, which control the expression of proteins involved in iron uptake, release and storage. Two cytoplasmic proteins with mRNA-binding properties, iron regulatory proteins 1 and 2 (IRP1 and IRP2) play a central role in this regulation. Foremost, IRPs regulate ferritin H and ...
Schmitt M M Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Campus North, Postfach 3640, Karlsruhe 76021, - - 2014
Wnt reception at the membrane is complex and not fully understood. CD44 is a major Wnt target gene in the intestine and is essential for Wnt-induced tumor progression in colorectal cancer. Here we show that CD44 acts as a positive regulator of the Wnt receptor complex. Downregulation of CD44 expression ...
Wang Qian Q Department of Clinical Laboratory Science, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, 214023, People's Republic of - - 2014
We previously reported that glucocorticoid receptor β (GRβ) regulates injury-mediated astrocyte activation and contributes to glioma pathogenesis via modulation of β-catenin/T-cell factor/lymphoid enhancer factor (TCF/LEF) transcriptional activity. The aim of this study was to characterize the mechanism behind cross-talk between GRβ and β-catenin/TCF in the progression of glioma. Here, we ...
Solaimani Parrisa P UCLA, United - - 2014
CYP1A1 bioactivates several procarcinogens and detoxifies several xenobiotic compounds. Transcription of CYP1A1 is highly induced by 2,3,7,8-tetrachlorodibenzo-р-dioxin (TCDD) via the aryl hydrocarbon receptor (AHR). We recently described an RNAi high throughput screening performed in the Hepa-1 mouse hepatoma cell line, which revealed that SIN3A is necessary for the induction of ...
Martin Elizabeth C - - 2014
The AKT/mammalian target of rapamycin (mTOR) signaling pathway is regulated by 17alpha-estradiol (E2) signaling and mediates E2-induced proliferation and progesterone receptor (PgR) expression in breast cancer.Methods and results: Here we use deep sequencing analysis of previously published data from The Cancer Genome Atlas to demonstrate that expression of a key ...
Dennis Stuart R SR Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield, UK, - - 2014
Elucidating the developmental and genetic control of phenotypic plasticity remains a central agenda in evolutionary ecology. Here, we investigate the physiological regulation of phenotypic plasticity induced by another organism, specifically predator-induced phenotypic plasticity in the model ecological and evolutionary organism Daphnia pulex. Our research centres on using molecular tools to ...
Lado Joanna J Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Valencia, Spain Instituto Nacional de Investigación Agropecuaria (INIA), - - 2014
Grapefruits are among the citrus species more sensitive to cold and develop chilling injury symptoms during prolonged postharvest storage at temperatures lower than 8 ℃-10 ℃. The plant hormone ethylene has been described either to protect or potentiate chilling injury development in citrus whereas little is known about transcriptional regulation of ethylene ...
Martineau Corine C Laboratoire du Métabolisme Osseux, BioMed, Département des Sciences Biologiques, Université du Québec à Montréal, Montréal, H3C 3P8, Quebec, - - 2014
Scavenger receptor class B type I (SR-BI), the Scarb1 gene product, is a high-density lipoprotein (HDL) receptor which was shown to influence bone metabolism. Its absence in mice is associated with alterations of the glucocorticoid/adrenocorticotropic hormone axis, and translated in high bone mass and enhanced bone formation. Since the cellular ...
Qin Qing Q Center for Translational Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA; Shanghai Institute of Cardiovascular Disease, Zhongshan Hospital, Fudan University, Shanghai 200032, - - 2014
Endothelin-1 (ET-1) produced by vascular endothelial cells plays essential roles in the regulation of vascular tone and development of cardiovascular diseases. The objective of this study is to identify novel regulators implicated in the regulation of ET-1 expression in vascular endothelial cells (ECs). By using quantitative real-time PCR (qRT-PCR) and ...
Francis W R WR Institute of Life Science, College of Medicine, Swansea University, UK. Electronic address: - - 2014
During bone maintenance in vivo, estrogen signals through Estrogen receptor (ER)-α. The objectives of this study were to investigate the temporal expression of ERα36 and ascertain its functional relevance during osteogenesis in human bone marrow derived mesenchymal stromal cells (BMSC). This was assessed in relation to runt-related transcription factor-2 (runx2), ...
Zhao Jiangang J Institute of Molecular Medicine, The University of Texas Health Science Center Medical School at Houston, Houston, TX 77030, - - 2014
Pluripotent embryonic stem cells (ESCs) generate rostral paraxial mesoderm-like progeny in 5-6 days of differentiation induced by Wnt3a and Noggin (Nog). We report that canonical Wnt signaling introduced either by forced expression of activated β-catenin, or the small-molecule inhibitor of Gsk3, CHIR99021, satisfied the need for Wnt3a signaling, and that the ...
Jang Young-Saeng YS Department of Microbiology, Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul 120-749, - - 2014
Endoglin (also known as CD105 or TGF-β type III receptor) is a co-receptor involved in TGF-β signaling. In atherosclerosis, TGF-β signaling is crucial in regulating disease progression owing to its anti-inflammatory effects as well as its inhibitory effects on smooth muscle cell proliferation and migration. Endoglin is a regulator of ...
1 2 3 4 5 6 7 8 9 10 >