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Cizkova Katerina K Department of Histology and Embryology; Palacky University; Olomouc, Czech - - 2014
There is growing evidence that some members of cytochrome P450 enzymes contribute to regulation of normal prenatal development. CYP epoxygenases (CYP2C and CYP2J subfamilies) convert arachidonic acid into four regioisomeric eicosatrienoic acids (EETs), biologically active molecules involved in mitogenesis and cell signaling. Almost nothing is known about localization of their ...
Mehus Aaron A AA University of North Dakota, United - - 2014
Human metallothioneins (MTs) are important regulators of metal homeostasis and protectors against oxidative damage. Their altered mRNA expression has been correlated with metal toxicity and a variety of cancers. Current immunodetection methods lack the specificity to distinguish all twelve human isoforms. Each, however, can be distinguished by the masses of ...
Li Wei W Yangzhou - - 2014
Sulfotransferase (SULT) 2A1 catalyzes sulfonation of drugs and endogenous compounds, and plays an important role in xenobiotic metabolism as well as in the maintenance of steroid and lipid homeostasis. A recent study showed that 17β-estradiol (E2) increases the mRNA levels of SULT2A1 in human hepatocytes. Here we report the underlying ...
Morató Roser R Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, 17071, Girona, - - 2014
One of the major obstacles for the vitrification of mature porcine oocytes with ethylene glycol is their low permeability to this cryoprotectant, which results in osmotic stress-induced cell damage and low survival. Pig blastocysts, on the other hand, show enhanced water and cryoprotectant permeability, which has been related to the ...
Na Hee Sam HS Department of Oral Microbiology, Pusan National University, Yangsan, 626-810, Republic of - - 2014
In the gingival tissues of patients with periodontitis, inflammatory responses are mediated by a wide variety of genes. In our previous screening study, plasminogen activator inhibitor type 1 (PAI-1) mRNA binding protein expression was increased in gingiva from periodontitis patients. In this study, we further investigated the signaling pathway involved ...
Zaga-Clavellina Veronica V Department of Cell Biology, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, - - 2014
To test the effect of PRL on expression of proinflammatory cytokines and MMP-9 in vitro. Tissue explants were incubated from 4 to 48 h alone or in the presence of 500 ng/mL PRL, and mRNA expression in tissues and secretion of IL-1β, TNF-α, MMP-2 and MMP-9 was quantified. Fetal membranes ...
Shibata M M Department of Orthodontics, Osaka University Dental Hospital, Suita, - - 2014
To investigate the effects of IL-17 on IL-6, IL-1β, and matrix metalloproteinase (MMP-1) production, and to compare the MMP-1 production between the individual and combined effects of IL-1β and IL-6 in human periodontal ligament fibroblasts (HPDLF). Human periodontal ligament fibroblasts were cultured with IL-17 for 0.5, 1, 4, 24, 48, ...
Vos Maria Caroline - - 2014
The aim of this study was to investigate the presence of MMP-14 and MMP-2 during human ovarian follicular development using immunohistochemistry, and the activity of MMP-2 in follicular fluid using zymography. Ovarian tissue collected from the archives of the Department of Pathology was examined and medical records and histopathology were ...
Jangani Maryam M From the Centre in Endocrinology and Diabetes, Institute of Human Development, - - 2014
Glucocorticoids (GC) regulate cell fate and immune function. We identified the metastasis-promoting methyltransferase, metastasis-related methyltransferase 1 (WBSCR22/Merm1) as a novel glucocorticoid receptor (GR) regulator relevant to human disease. Merm1 binds the GR co-activator GRIP1 but not GR. Loss of Merm1 impaired both GR transactivation and transrepression by reducing GR recruitment ...
Weng Chih-Ming CM Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, - - 2014
Endothelin-1 (ET-1) acts as a key mediator of vasoconstriction and tissue repair. Overproduction of connective tissue growth factor (CTGF) underlies the development of lung fibrosis. ET-1 induces expression of matrix-associated genes in lung fibroblasts, however, little is known about the signaling pathway of CTGF expression caused by ET-1. In this ...
Lyros Orestis O Division of Gastroenterology and Hepatology, Medical College of Wisconsin, Milwaukee, - - 2014
Squamous esophageal epithelium adapts to acid reflux-mediated injury by proliferation and differentiation via signal transduction pathways. Induction of the Wnt antagonist Dickkopf-1 (Dkk1) is involved in tissue repair during inflammation and cellular injury. In this study, we aimed to identify the biological role of Dkk1 in human reflux esophagitis with ...
Jung Hyejung H Department of Life Sciences, Division of Life and Pharmaceutical Sciences and the Center for Cellular Homeostasis, Ewha Womans University, Seoul, 120-750, - - 2014
Syndecan-2, a transmembrane heparan sulfate proteoglycan that is highly expressed in melanoma cells, regulates melanoma cell functions (e.g., migration). Since melanoma is a malignant tumor of melanocytes, which largely function to synthesize melanin, we investigated the possible involvement of syndecan-2 in melanogenesis. Syndecan-2 expression was increased in human skin melanoma ...
Davidson Lee L Wellcome Trust Centre for Cell Biology, Edinburgh EH9 3JR, United - - 2014
3' end formation of pre-mRNAs is coupled to their transcription via the C-terminal domain (CTD) of RNA polymerase II (Pol II). Nearly all protein-coding transcripts are matured by cleavage and polyadenylation (CPA), which is frequently misregulated in disease. Understanding how transcription is coordinated with CPA in human cells is therefore ...
Wang Ke K Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, Jiangsu Province, China. Electronic address: - - 2014
Ciliary neurotrophic factor (CNTF) is characterized as a neuropoietic cytokine for a broad spectrum of neurons, leading to its evaluation in humans suffering from neurodegenerative diseases. Due to its wide range of biological applications, high yield production of soluble biologically active recombinant human CNTF (rhCNTF) in heterologous expression system is ...
Ohata Yasuhisa Y Department of Bone and Mineral Research, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka, 594-1101, Japan; Department of Pediatrics, Osaka University Graduate School of Medicine, Suita, Osaka, 565-0871, - - 2014
Fibroblast growth factor 23 (FGF23) functions in an endocrine fashion and requires α-Klotho to exert its effects on the target organs. We have recently demonstrated that the human placenta also expresses α-Klotho, which led us to hypothesize that FGF23 may exert effects on the placenta. Immunohistochemical analysis demonstrated the expression ...
Han Limin L Peking University Research Center on Aging, Peking University Health Science Center, Beijing, 100191, People's Republic of China; Department of Biochemistry & Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, People's Republic of - - 2014
SIRT1 is a key protein in mediating the benefits of calorie restriction (CR) in mammals. However, the molecular mechanisms of CR-induced SIRT1 up-regulation in mammals remain unclear. We show that the elevated SIRT1 level is not attributed to the increased SIRT1 mRNA levels, but the enhanced SIRT1 protein stability via ...
Haseeb Abdul A Northeast Ohio Medical University, United - - 2014
5-hydroxymethylcytosine (5-hmC) generated by ten-eleven translocation 1-3 (TET1-3) enzymes is an epigenetic mark present in many tissues with different degrees of abundance. IL-1β and TNF-α are the two major cytokines present in arthritic joints that modulate the expression of many genes associated with cartilage degradation in osteoarthritis (OA). In the ...
Ghonem Nisanne S NS Department of Internal Medicine, Liver Center, Yale University School of Medicine, New Haven, - - 2014
Multidrug resistance transporter 3/ATP-binding cassette protein subfamily B4 (MDR3/ABCB4) is a critical determinant of biliary phosphatidylcholine (PC) secretion. Clinically, mutations and partial deficiencies in MDR3 result in cholestatic liver injury. Thus, MDR3 is a potential therapeutic target for cholestatic liver disease. Fenofibrate is a peroxisome proliferator-activated receptor (PPAR) α ligand ...
de Lucas Susana S Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología (CSIC), C/Darwin 3, Campus Cantoblanco, 28049 Madrid, Spain, Ciber de Enfermedades Respiratorias (ISCIII), Mallorca, Spain, Servicio de Genómica Computacional, Centro Nacional de Biotecnología (CSIC), C/Darwin 3, Campus Cantoblanco, 28049 Madrid, Spain and Bioinformática de Sistemas, Centro Nacional de Biotecnología (CSIC), C/Darwin 3, Campus Cantoblanco, 28049 Madrid, - - 2014
Cellular messenger RNAs (mRNAs) are associated to proteins in the form of ribonucleoprotein particles. The double-stranded RNA-binding (DRB) proteins play important roles in mRNA synthesis, modification, activity and decay. Staufen is a DRB protein involved in the localized translation of specific mRNAs during Drosophila early development. The human Staufen1 (hStau1) ...
Scotece Morena M SERGAS, Research Laboratory 9 (NEIRID LAB: Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases), Institute of Medical Research (IDIS), Santiago University Clinical Hospital, Santiago de Compostela, 15706, - - 2014
Nesfatin-1 is a recently discovered satiety-inducing adipokine identified in hypothalamic regions that regulates energy balance. So far, no data exist on NUCB2/nesfatin-1 localization in human and murine chondrocytes. Here, we therefore investigated NUCB2/nesfatin-1 gene and protein expression in human and murine chondrocytes and the effect of nesfatin-1 on pro-inflammatory cytokines ...
Nakamura Yasuhiro Y Department of Pathology, Tohoku University School of Medicine, Sendai, Japan. Electronic address: - - 2014
Aberrant expression of gonadotropin-releasing hormone receptor (GnRHR) has been reported in human adrenal tissues including aldosterone-producing adenoma (APA). However, the details of its expression and functional role in adrenals are still not clear. In this study, quantitative RT-PCR analysis revealed the mean level of GnRHR mRNA was significantly higher in ...
Zhang Ruifeng R 1Department of Respiratory Medicine, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, - - 2014
We previously observed that transgelin was preferentially expressed in human pulmonary arterial smooth muscle cells (PAMSCs) under hypoxia and that the upregulation of transgelin was independent of hypoxia inducible factor 1α (HIF-1α). Reduced transgelin expression was accompanied by significantly impaired migration ability in vitro. However, the regulation mechanism of transgelin ...
Li Rui R Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan 250012, PR China; Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan 250012, PR - - 2014
Secretory human interleukin 4 (hIL4) is an N-glycosylated pleiotropic cytokine. It is unknown if these N-linked glycans are required and essential for hIL4 protein stability, expression, secretion, and activity in vivo, and hIL4 expressed from Pichia pastoris yeast has not been tested to date. In this study, we successfully expressed ...
Chi J J Division of Cardiovascular Medicine, Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Shimoaizuki, Matusoka, Eiheji-Cho, Fukui, - - 2014
The aim of this study was to determine the influence of high glucose (HG) and interleukin (IL)-1β on human cardiac fibroblast (HCF) functions, and to evaluate the effects of eplerenone in these responses. HCFs were cultured in normal or HG media in the absence or presence of IL-1β and/or eplerenone. ...
Verstraelen Jessica - - 2014
Preclinical studies addressing the transcorneal absorption of ophthalmic drugs are mainly performed using ex vivo animal corneas and in vitro corneal cell culture models, leaving open the question of transferability to humans in an in vivo situation. While passive drug absorption through corneal tissue is well understood, little is known ...
Ludwig Anna Lisa AL Department of Biochemistry and Molecular Medicine, University of California, Davis, School of Medicine, Davis, CA, - - 2014
Large expansions of a CGG-repeat element (>200 repeats; full mutation) in the fragile X mental retardation 1 (FMR1) gene cause fragile X syndrome (FXS), the leading single-gene form of intellectual disability and of autism spectrum disorder. Smaller expansions (55-200 CGG repeats; premutation) result in the neurodegenerative disorder, fragile X-associated tremor/ataxia ...
Helan Martin M Department of Anesthesiology, Mayo Clinic, Rochester, USA; ICRC, International Clinical Research Center, Brno, Czech Republic; Department of Anesthesiology and Intensive Care, St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Brno, Czech - - 2014
Within human pulmonary artery, neurotrophin growth factors [NTs; e.g. brain-derived neurotrophic factor (BDNF)] and their high-affinity receptors (tropomyosin-related kinase; Trk) and low-affinity receptors p75 neurotrophin receptor (p75NTR) have been reported, but their functional role is incompletely understood. We tested the hypothesis that BDNF is produced by human pulmonary artery endothelial ...
Tsai Hsiao-Chi HC Department of Life Sciences, National Chung Hsing University, Taichung, - - 2014
Osteosarcoma is the most common primary malignant tumor, and its treatments require more effective therapeutic approaches. Paclitaxel has a broad range of antitumor activities, including apoptosis-inducing effects. However, the majority of tumors in patients with advanced cancer eventually develop chemoresistance. Connective tissue growth factor (CTGF) is a secreted protein that ...
Flores Omar O Department of Molecular Genetics & Microbiology and Center for Virology, Duke University Medical Center, Durham, NC 27710, - - 2014
It has previously been assumed that the generally high stability of microRNAs (miRNAs) reflects their tight association with Argonaute (Ago) proteins, essential components of the RNA-induced silencing complex (RISC). However, recent data have suggested that the majority of mature miRNAs are not, in fact, Ago associated. Here, we demonstrate that ...
Degoricija Marina M Department of Immunology, School of Medicine, University of Split, Šoltanska 2, 21000, Split, - - 2014
Given that the tumor-promoting inflammation has been previously established in squamous cell carcinoma of the bladder but its contribution to development of urothelial carcinoma (UC) still remains elusive, our aim was to study changes in expression and activity of inflammation-mediating NF-κB and STAT3 transcription factors in human urothelial bladder carcinoma ...
Ebeling Sandra S Pharmaceutical Biology and Biotechnology, Albert-Ludwigs-University Freiburg, Freiburg, - - 2014
Birch bark has a long lasting history as a traditional medicinal remedy to accelerate wound healing. Recently, the efficacy of birch bark preparations has also been proven clinically. As active principle pentacyclic triterpenes are generally accepted. Here, we report a comprehensive study on the underlying molecular mechanisms of the wound ...
Crociani Olivia O Dept of Experimental Pathology and Oncology, University of - - 2014
hERG1 channels are aberrantly expressed in several types of human cancers, where they affect different aspects of cancer cell behaviour. A thorough analysis of the functional role and clinical significance of hERG1 channels in gastric cancer (GC) is still lacking. Experimental design: hERG1 expression was tested in a wide (508 ...
Litzenburger Ulrike M UM Department of Neurooncology, Neurology Clinic and National Center for Tumor Diseases University Hospital of Heidelberg, Heidelberg, - - 2014
Indoleamine-2,3-dioxygenase (IDO) inhibitors have entered clinical trials based on their ability to restore anti-tumor immunity in preclinical studies. However, the mechanisms leading to constitutive expression of IDO in human tumors are largely unknown. Here we analyzed the pathways mediating constitutive IDO expression in human cancer. IDO-positive tumor cells and tissues ...
Mosher Andrea A AA 1Department of Physiology & Pharmacology, Alberta Children's Hospital Research Institute for Child and Maternal Health, Faculty of Medicine, University of Calgary, Calgary, Alberta, - - 2014
Prostaglandins are implicated in the labor process, yet the precise role and regulation of the prostaglandin pathway remains to be elucidated. The first step in the pathway is cleavage of membrane phospholipids by phospholipase A2 (PLA2). Previous work demonstrated upregulation of secretory PLA2 (sPLA2)-IIA with labor in human myometrium, and ...
Hsieh Yu-Ping YP Department of School of Dentistry, National Taiwan University, - - 2014
Background. Early growth response-1 (Egr-1) protein plays an important role in many human fibrotic diseases. Areca nut (AN) chewing is the most important risk factor of oral submucous fibrosis (OSF). Methods. Egr-1 protein expression in OSF was examined using antibody to Egr-1. Arecoline-induced Egr-1 expression and its signaling pathways were ...
Martínez Víctor G VG Department of Cell Biology, Faculty of Medicine, Complutense University, Madrid, - - 2014
Bone morphogenetic proteins (BMPs) are multifunctional growth factors regulating differentiation and proliferation in numerous systems including the immune system. Previously, we described that the BMP signaling pathway is functional in human monocyte-derived dendritic cells (MoDCs), which were found to express both the specific receptors and the Smad proteins required for ...
Chang Hui-Hsin - - 2014
A C-to-T single nucleotide polymorphism (SNP) located at position 1858 of human protein tyrosine phosphatase, non-receptor type 22 (PTPN22) complementary DNA (cDNA) is associated with an increased risk of systemic lupus erythematosus (SLE). How the overall activity of PTPN22 is regulated and how the expression of PTPN22 differs between healthy ...
Cuesta Antonio A Departamento de Morfología y Biología - - 2014
Abstract Acid-sensing ion channels (ASICs) are a family of H(+)-gated voltage-insensitive ion channels that respond to extracellular acidification by regulating transmembrane Ca(2+) flux. Moreover, ASICs can be also gated by mechanical forces and may function as mechanosensors. The cells of the intervertebral disc (IVD) have an unusual acidic and hyperosmotic ...
Chai S Y SY Mothers and Babies Research Centre, Hunter Medical Research Institute, University of Newcastle, Newcastle, - - 2014
Progesterone regulates female reproductive function predominantly through two nuclear progesterone receptors (PRs), PR-A and PR-B. During human parturition myometrial PR expression is altered to favour PR-A, which activates pro-labour genes. We have previously identified histone H3 lysine 4 trimethylation (H3K4me3), as an activator of myometrial PR-A expression at labour. To ...
Narasimhan Sukanya S Section of Infectious Diseases, Department of Internal Medicine, Yale University, New Haven, CT 06520, USA. Electronic address: - - 2014
Arthopods such as Ixodes scapularis ticks serve as vectors for many human pathogens. The arthropod gut presents a pivotal microbial entry point and determines pathogen colonization and survival. We show that the gut microbiota of I. scapularis, a major vector of the Lyme disease spirochete Borrelia burgdorferi, influence spirochete colonization of ...
Scharenberg Matthias A - - 2014
Cellular transformation into myofibroblasts is a central physiological process enabling tissue repair. Its deregulation promotes fibrosis and carcinogenesis. TGF-β is the main inducer of the contractile gene program that drives myofibroblast differentiation from various precursor cell types. Crucial regulators of this transcriptional program are SRF and its cofactor MKL1/MRTF-A. However, ...
Walters M J MJ ChemoCentryx Inc., 850 Maude Ave, Mountain View, CA 94043, - - 2014
In experimental models of glioblastoma multiforme (GBM), irradiation (IR) induces local expression of the chemokine CXCL12/SDF-1, which promotes tumour recurrence. The role of CXCR7, the high-affinity receptor for CXCL12, in the tumour's response to IR has not been addressed. We tested CXCR7 inhibitors for their effects on tumour growth and/or ...
Sakabe Jun-Ichi JI Department of Dermatology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan. jsakabe@med.uoeh-u.ac.jp, - - 2014
Interleukins (IL)-17A and -22 are involved in the patho-genesis of psoriasis. Cathelicidin LL37 serves as not only antimicrobial peptide but also as autoinflammatory mediator. 1,25-Dihydroxyvitamin D3 analogues, such as calcipotriol, are used as topical treatment for psoriasis. However, the effect of calcipotriol on the mRNA expression/production of human cathelicidin antimicrobial ...
Yang Tian-Quan TQ Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu, 215006, - - 2014
Recent studies have identified a class of small non-coding RNA molecules, named microRNA (miRNA), which are dysregulated in malignant brain glioblastoma. Substantial data have indicated that miRNA-16 (miR-16) plays a significant role in tumors of various origins. This miRNA has been involved in various aspects of carcinogenesis, including cell apoptosis ...
Lin Changpo - - 2014
Acute myocardial infarction and stroke are more likely to occur in the early morning. Circadian pacemakers are considered to be involved in the process. Many peripheral tissues and cells also contain clock systems. In this study, we examined whether the primary cultured human plaque-derived vascular smooth muscle cells (VSMCs) process ...
Cha Byung-Hyun BH Department of Biomedical Science, College of Life Science, CHA University, Yatap-Dong, Bundang-Gu, Seongnam-Si, Gyeonggi-Do 463-840, Republic of - - 2014
Obesity has become a serious public health problem in the developed world. Increased mass of adipose tissue in the obese is due to an increase in both the size (hypertrophy) and number (hyperplasia) of adipocytes. The chemical chaperone tauroursodeoxycholic acid (TUDCA) not only decreases endoplasmic reticulum (ER) stress, but also ...
Hsu Chih-Kai CK 1College of Medicine, Chang Gung - - 2014
Elevated levels of TNF-α have been detected in the airway fluids, which may induce up-regulation of inflammatory proteins. Suppressors of cytokine signaling (SOCS)-3 proteins can be induced by various cytokines and negatively regulated inflammatory responses. Although TNF-α has been shown to induce SOCS-3 expression, the mechanisms underlying TNF-α-induced SOCS-3 expression ...
Sankar Natesan N Loyola Universtiy Chicago, United - - 2014
In heart, the type-2 Inositol 1,4,5-triphosphate receptor (InsP3R2) is the predominant isoform expressed and is localized in the nuclear membrane of ventricular myocytes. InsP3R2 mediated Ca2+ release regulates hypertrophy specific gene expression by modulating CaMKIIδ, HDAC and calcineurin-NFATc signaling pathways. InsP3R2 protein is a hypertrophy specific marker and is over-expressed ...
Lee Jin Kyung JK University of Illinois at - - 2014
The state of pregnancy is known to alter hepatic drug metabolism. Hormones that rise during pregnancy are potentially responsible for the changes. Here we report the effects of prolactin (PRL), placental lactogen (PL), and growth hormone variant (GH-v) on expression of major hepatic cytochromes P450 (CYP) expression and a potential ...
Idogawa Masashi M Department of Medical Genome Sciences, Research Institute for Frontier - - 2014
p53 is one of the most important known tumor suppressor genes, and it is inactivated in approximately half of human cancers. p53 family members execute various functions, such as apoptosis induction and cell cycle arrest, by modulating transcriptional regulation. Therefore, the direct transcriptional targets of the p53 family must be ...
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