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Results 201 - 250 of 3382
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Lee So-Young SY From the Department of Pathology, Asan Medical Center (Drs S-Y. Lee, C. J. Kim, K-R. Kim, and C-S. Park), and Asan Institute for Life Science (Ms H-S. Lee and Dr Gil), University of Ulsan, College of Medicine, Seoul, and Department of Pathology, University of Ajou, College of Medicine, Ajou University Hospital, Su-won (Dr Y-H. Lee), - - 2014
Context.-The ability of intermediate trophoblasts to invade maternal tissue during placentation depends on how well they can degrade the extracellular matrix. Invasion into the extracellular matrix requires many complex proteases. A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) is a novel family of secreted metalloproteinases. The ADAMTS-1, -4, -5, and ...
Liu Rong-Diao RD School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, - - 2014
After reverse transcription, the HIV-1 proviral DNA is integrated into the host genome and thus subjected to transcription by the host RNA polymerase II (Pol II). With the identification and characterization of human P-TEFb in the late 1990s as a specific host cofactor required for HIV-1 transcription, it is now ...
Sun Kai K Department of General Surgery, Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong 510515, China. Email: - - 2014
miR-338-3p is a recently discovered miRNA and is involved in cell differentiation. However, few data are yet available on the aberrant expression of miR-338-3p in human colorectal carcinoma (CRC). This work aimed to investigate the relationship between miR-338-3p expression pattern and clinicopathological features of human CRC and the possible regulative ...
Shi Lihong L Department of Cell and Developmental Biology - - 2014
To globally survey the changes in transcriptional landscape during terminal erythroid differentiation, we performed RNA sequencing (RNA-seq) on primary human CD34(+) cells after ex vivo differentiation from the earliest into the most mature erythroid cell stages. This analysis identified thousands of novel intergenic and intronic transcripts as well as novel ...
Harada Masanori M Department of Molecular Biology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan; Second Department of Internal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, - - 2014
Cyclin D1, an oncogenic G1 cyclin, and YB-1, a transcription factor involved in cell growth, are both over-expressed in several human cancers. In human lung cancer, the functional association between YB-1 and cyclin D1 has never been elucidated. In this study, we show YB-1 is involved in the transcription of ...
Davidge Pitts Caroline C C Davidge Pitts, Endocrinology, Mayo Clinic, Rochester, United - - 2014
Fat distribution differs between individuals, and those with visceral fat predominance develop metabolic profiles that increase risk of adverse cardiovascular events. This is due, in part, to the proinflammatory state associated with visceral obesity as well as depot-specific adipogenesis. The insulin-like growth factor (IGF) system is important in adipose tissue ...
Salas-Huetos Albert A Unitat de Biologia Cel·lular, Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), - - 2014
To characterize the microRNA (miRNA) expression profile in spermatozoa from human fertile individuals and their implications in human fertility. The expression levels of 736 miRNAs were evaluated using TaqMan arrays. Ontologic analyses were performed to determine the presence of enriched biological processes among their targets. University research and clinical institutes. ...
Sun Lei L School of Pharmacy, Shanghai Jiao Tong University, Shanghai, - - 2014
Interleukin-33 (IL-33), an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. However, little is known about the induced expression of IL-33 in monocytes and macrophages, which are major cytokine-producing cells of the innate immune system. Here we report the induction of ...
Liu Te T Shanghai Tenth People's Hospital, Medical School, Tongji University, Shanghai, 200072, China, - - 2014
The Polycomb group protein, enhancer of zeste homolog 2 (EZH2), plays an important role in transcriptional regulation through chromatin remodeling and interactions with other transcription factors to control cell proliferation and embryonic development. Previous study has shown that EZH2 is important for cell cycle regulation and is highly expressed in ...
Fry Rebecca C RC 1University of North - - 2014
Background: Ozone (O3) is a criteria air pollutant that is associated with numerous adverse health effects, including altered respiratory immune responses. Despite its deleterious health effects, possible epigenetic mechanisms underlying O3-induced health effects remain understudied. MicroRNAs (miRNAs) are epigenetic regulators of genomic response to environmental insults and unstudied in relationship ...
Feng Ying Y State Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China; School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325002, - - 2014
The canonical Wnt signaling pathway plays critical roles during development and homeostasis. Dysregulation of this pathway can lead to many human diseases, including cancers. A key process in this pathway consists of regulation of β-catenin concentration through an Axin-recruited destruction complex. Previous studies have demonstrated a role for Tankyrase (TNKS), ...
Guo Jia J School of Biomedical Sciences; Faculty of Medicine; The Chinese University of Hong Kong; Hong Kong, P.R. - - 2014
The microphthalmia-associated transcription factor (MITF) is a pivotal regulator of melanogenic enzymes for melanogenesis, and its expression is modulated by many transcriptional factors at the transcriptional level or post-transcriptional level through microRNAs (miRNAs). Although several miRNAs modulate melanogenic activities, there is no evidence of their direct action on MITF expression. ...
Chuderland Dana D Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Ramat-Aviv, Tel-Aviv 69978, - - 2014
Pigment epithelium-derived factor (PEDF) is highly expressed in the female reproductive system and is subjected to regulation by steroid hormones in the ovary. As the uterine endometrium exhibits morphological and functional changes in response to estrogen (E2) and progesterone (P4), we aimed at characterizing the expression of PEDF in this ...
Tillmann Frank-Peter FP Klinik für Nephrologie, Heinrich-Heine Universität , Düsseldorf, - - 2014
The importance of fibroblast growth factor (FGF)-23 as part of a hormonal bone-kidney-axis has been well established. Lately, FGF-23 has been suggested as an independent risk factor of death in patients on chronic hemodialysis. Hyperparathyroidism is a common feature of advanced kidney failure or end-stage renal disease. The independent effect ...
Sha Xiangyin X Department of Ophthalmology, The Second Affiliated Hospital of Guangzhou Medical University , Guangzhou , - - 2014
Abstract Purpose: Epithelial-mesenchymal transition (EMT) of normal conjunctival tissues is a major reason of pterygium generation; α-smooth muscle actin (α-SMA) is a maker in EMT. In this study, we investigated if human amniotic mesenchymal stem cells (hAMSCs) can inhibit α-SMA expression and migration of human pterygium fibroblasts (hPFs) in vitro. ...
Liu Feng-Jun FJ College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China. Electronic address: - - 2014
Placental deficiencies are related to the developmental abnormalities of transgenic cattle produced by somatic cell nuclear transfer, but the concrete molecular mechanism is not very clear. Studies have shown that placental development can be regulated by microRNAs (miRNAs) in normal pregnancy. Thus, this study screened differentially expressed miRNAs by the ...
Taylor Cormac T CT School of Medicine and Medical Science, The Conway Institute, University College Dublin, - - 2014
Intermittent hypoxia (IH)-induced activation of pro-inflammatory pathways is a major contributing factor to the cardiovascular pathophysiology associated with obstructive sleep apnoea (OSA). Obesity is commonly associated with OSA although it remains unknown whether adipose tissue is a major source of inflammatory mediators in response to IH. The aim of this ...
Ma Bo B Medical School of Xi'an Jiaotong University, First Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an 710061, - - 2014
Transforming growth factor (TGF)-β2, gremlin and connective tissue growth factor (CTGF) are known to play important roles in the induction of epithelial mesenchymal transition (EMT) and extracellular matrix (ECM) synthesis. However, the complex functional relationship among gremlin, CTGF and TGF-β2 in the induction of EMT and ECM synthesis in human ...
Sousa Sílvia Ferreira de SF Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.. Electronic address: - - 2014
Protein kinase A (PKA) plays critical roles in many biological processes including cell proliferation, cell differentiation, cellular metabolism and gene regulation. Mutation in PKA regulatory subunit, PRKAR1A has previously been identified in odontogenic myxomas, but it is unclear whether PKA is involved in tooth development. The aim of the present ...
Lim Ratana R Mercy Perinatal Research Centre, Mercy Hospital for Women, Heidelberg, Victoria, Australia; Obstetrics, Nutrition and Endocrinology Group, Department of Obstetrics and Gynaecology, University of Melbourne, Victoria, - - 2014
The aims of this study were (i) to determine the localisation of activator protein (AP)-1 family members (cFos, FosB, cJun, JunB and JunD) in human myometrium; and (ii) to determine the effect of human term labour on the expression of AP-1 family of transcription factors in myometrium. This localised the ...
Xiaoyan Li L Department of Pathology and Institute of Oncology, Fujian Medical University, Fuzhou 350004, PR China; Department of Pathology, Fujian Medical University Union Hospital, Fuzhou 350001, PR - - 2014
To explore the expression and role of activating transcription factor 3 in human hepatocellular carcinoma. Immunohistochemistry, Western blot assay and Real-time PCR were used to evaluate activating transcription factor 3 protein and gene level in HCC clinical samples. Activating transcription factor 3 expression is lowest in HCC, and the protein ...
Shi Hengliang H Department of Neurosurgery, Affiliated Hospital of Xuzhou Medical College, Jiangsu, People's Republic of - - 2014
Recently, calcyclin-binding protein or Siah-1-interacting protein (CacyBP/SIP), a component of a novel ubiquitinylation pathway, could regulate the β-catenin degradation (Fukushima et al., Immunity 2006, 24, 29 - 39). However, the potential role of CacyBP/SIP itself in human glioma cells has not been clarified. Here, we found that CacyBP/SIP was expressed ...
Troppmann B B Centre of Reproductive Medicine and Andrology, University Hospital Muenster, Albert-Schweitzer-Campus 1, 48149 Muenster, - - 2014
The luteinizing hormone/chorionic gonadotropin receptor (LHCGR) is essential for normal male and female reproductive processes. The spatial and temporal LHCGR gene expression is controlled by a complex system of regulatory mechanisms which are crucial for normal physiological function, especially during the female cycle. In this study, we aimed to elucidate ...
Xing Deguang D Department of Neurosurgery, The First Hospital of China Medical University, Shenyang 110001, P.R. - - 2014
In the present study, we designed and conducted a series of assays to determine the expression of voltage-gated sodium channel (VGSC) neonatal isoform Nav1.5 (nNav1.5) in human brain astrocytoma and its effect on the proliferation, migration, invasion and apoptosis of astrocytoma U251 cells. The results showed that nNav1.5 mRNA and ...
Pfitzner Berit Maria BM Institute of Pathology, Campus Mitte, Charité University Hospital Berlin, Charitéplatz 1, 10117, Berlin, Germany, - - 2014
RANK ligand (RANKL) is crucial for the development of mouse mammary glands during pregnancy. RANKL functions as a major paracrine effector of the mitogenic action of progesterone in mammary epithelium via its receptor RANK and has a role in expansion and regenerative potential of mammary stem cells. Pharmacologic inhibition of ...
Kim Mi-Ryung MR OURHOME Co. Ltd Food R&D Center, Sungnam, 462-819, Republic of - - 2014
UVA is responsible for numerous biological effects on the skin, including premature aging characterized by wrinkles, leathery texture, and mottled pigmentation. The objective of this study was evaluating the protective effect of ginseng leaf extract prepared by Ultraflo L on skin from photodamage. Anti-wrinkle effect of ginseng leaf extract with ...
Ovando-Roche Patrick P Institute of Reproductive and Developmental Biology, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London, W12 0NN, - - 2014
Telomere repeat binding factor 2 (TRF2) is a component of the shelterin complex that is known to bind and protect telomeric DNA, yet the detection of TRF2 in extra-telomeric regions of chromosomes suggests other roles for TRF2 besides telomere protection. Here, we demonstrate that TRF2 plays a critical role in ...
Wolf Ashley R AR Howard Hughes Medical Institute, Department of Molecular Biology, and Center for Human Genetic Research, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA; Broad Institute, Cambridge, MA 02141, - - 2014
Both strands of human mtDNA are transcribed in continuous, multigenic units that are cleaved into the mature rRNAs, tRNAs, and mRNAs required for respiratory chain biogenesis. We sought to systematically identify nuclear-encoded proteins that contribute to processing of mtRNAs within the organelle. First, we devised and validated a multiplex MitoString assay ...
Shindoh Hisayuu H Department of Obstetrics and Gynecology, Kansai Medical University, Osaka, - - 2014
To investigate the role of heart and neural crest derivatives-expressed transcript 2 (HAND2) during decidualization of human endometrial stromal cells (ESCs). In vitro experiment. Research laboratory. Twenty-six patients undergoing hysterectomy for benign reasons. ESCs were cultured for 12 days with HAND2 small interfering RNA (siRNA) or nonsilencing RNA during decidualization by medroxyprogesterone ...
Takagi Toshinori T Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan; Department of Neurosurgery, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan. Electronic address: - - 2014
Oxidative stress plays a critical role in mediating tissue injury and neuron death during ischemia-reperfusion injury (IRI). The Keap1-Nrf2 defense pathway serves as a master regulator of endogenous antioxidant defense, and Nrf2 has been attracting attention as a target for the treatment of IRI. In the present study, we evaluated ...
Yao Weiwu W Department of Radiology, Shanghai Jiao Tong University Sixth People's Hospital, Shanghai, 200233, - - 2014
Knee osteoarthritis (OA) is a major cause of disability, particularly in the elderly. The aim of this study was to investigate the role of osteopontin (OPN) in the pathogenesis of OA by studying the effect of OPN on expression of IL-6 and IL-8 inflammatory factors in human knee OA chondrocytes. ...
Liu X X Department of Obstetrics and Gynecology, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, - - 2014
Well-controlled trophoblast migration and invasion at the maternal-foetal interface are crucial events for normal placentation and successful pregnancy. Growing evidence has revealed that growth arrest and DNA damage-inducible 45 alpha (Gadd45α) participates in tumour migration and invasion as a tumour suppressor. However, the expression and function of Gadd45α in trophoblasts ...
Reyes-Quiroz María Edith ME Departamento de Bioquímica Médica y Biología Molecular, Facultad de Medicina, Universidad de Sevilla, Avda. Sánchez Pizjuán 4, 41009, Sevilla, - - 2014
Regulation of liver X receptors (LXRs) is essential for cholesterol homeostasis and inflammation. The present study was conducted to determine whether oleic acid (OA) could regulate mRNA expression of LXRα and LXRα-regulated genes and to assess the potential promotion of oxidative stress by OA in neutrophils. Human neutrophils were treated ...
Cui Huachun H Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, - - 2014
Long noncoding RNAs (lncRNAs), once thought to be transcriptional noise, have been recently shown to regulate a variety of biological processes. However, there is not much knowledge regarding their roles in inflammatory response. In this study, we performed human lncRNA microarray assays and identified a number of lncRNAs that demonstrated ...
Ramirez Cristina M CM Yale University School of Medicine, United - - 2014
The ATP-binding cassette transporter A1 (ABCA1) is a major regulator of cellular cholesterol efflux and plasma high-density lipoprotein (HDL) biogenesis. Even though the transcriptional activation of ABCA1 is well established, the post-transcriptional regulation of ABCA1 expression is poorly understood. Here, we investigate the potential contribution of the Ribonucleoprotein-binding protein (RIP), ...
Schicht Martin - - 2014
Sex hormones, especially estrogens, have been implicated in articular cartilage metabolism and the pathogenesis of postmenopausal osteoarthritis (OA). Conversion by aromatase (CYP19A1) of androstendione into estrone (E1) and testosterone into 17beta-estradiol (E2) plays a key role in tissues that synthesize estrogens endogenously. We analyzed the expression of aromatase (CYP19A1) in ...
Schweiger Nicole N Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Vienna, - - 2014
Fibroblast growth factor receptors (FGFRs) are important in malignant progression of several human epithelial tumors. However, little is known about FGFRs in canine or human soft tissue sarcomas. Thus, our aim was to investigate expression of FGFRs and their involvement in cell survival in sarcomas of both species. FGFR1-4 and ...
Li Lupeng - - 2014
The molecular mechanisms responsible for angiogenesis and abnormal expression of angiogenic factors in gastric cancer, including vascular endothelial growth factor (VEGF), remain unclear. The histone demethylase retinoblastoma binding protein 2 (RBP2) is involved in gastric tumorgenesis by inhibiting the expression of cyclin-dependent kinase inhibitors (CDKIs). The expression of RBP2, VEGF, ...
Fang Lanlan L Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China - - 2014
Context: Cyclooxygenase-2 (COX-2) expression and prostaglandin E2 (PGE2) production have been shown to play key roles in the regulation of ovulation. The transforming growth factor-beta (TGF-β) superfamily members are important molecules that regulate many ovarian functions under normal physiological and pathological conditions. TGF-β1 and its receptors are expressed in human ...
Gomes Débora C DC School of Medicine of Ribeirao Preto-University of Sao Paulo, Brazil 1 - - 2014
Background: The sonic hedgehog (SHH) pathway plays a key role in rodent adrenal cortex development and is involved in tumorigenesis in several human tissues, but data in human adrenal glands are limited. Objectives: To analyze the involvement of the SHH pathway in human adrenal development and tumorigenesis and the effects ...
Tewari Angela A King's College London(KCL), King's College London School of Medicine, Division of Genetics and Molecular Medicine, St John's Institute of Dermatology, London, - - 2014
UVA1 makes up around 75% of terrestrial ultraviolet radiation (UVR), and most of the output of artificial tanning sources. However, the molecular effects of UVA1 in human skin in vivo are surprisingly poorly understood. We have examined time dependent whole genome expression, mRNA and protein changes in the skin after ...
Kämpfer N N Institute of Pharmacology & Toxicology, University of Bonn, Biomedical Center, Sigmund-Freud-Str. 25, D-53105, Bonn, - - 2014
Based on their bronchodilatory effect, β2-adrenoceptor agonists constitute essential elements in the treatment of bronchial asthma and COPD. As treatment with β2-adrenoceptor agonists has been associated with worsening of airway hyper-reactivity, possibly because of loss of β-adrenoceptor function, molecular mechanism of the regulation of β2-adrenoceptor expression were studied. MRC-5 human ...
Song Qiancheng Q Department of Cell Biology, School of Basic Medical Sciences, Southern Medical - - 2014
The nodal regulatory properties of microRNAs in metastatic cancer may offer new targets for therapeutic control. Here we report that upregulation of miR-483-5p is correlated with the progression of human lung adenocarcinoma. miR-483-5p promotes epithelial-mesenchymal transition (EMT) accompanied by invasive and metastatic properties of lung adenocarcinoma. Mechanistically, miR-483-5p is activated ...
Friedländer Marc R - - 2014
MicroRNAs (miRNAs) are established regulators of development, cell identity and disease. Although nearly two thousand human miRNA genes are known and new ones are continuously discovered, no attempt has been made to gauge the total miRNA content of the human genome. Employing an innovative computational method on massively pooled small ...
Kazantseva Jekaterina J Protobios LLC, Mäealuse 4, Tallinn, 12618, Estonia, - - 2014
Expression of general transcription factor and co-activator TAF4 varies during development and in the processes of cell differentiation with suggested connection to neurodegenerative diseases. Here, we show that expression of TAF4 alternative splice variants is different in various regions of the human brain, substantiating the role of alternative splicing of ...
Rosa Alessandro A Laboratory of Molecular Vertebrate Embryology, The Rockefeller University, New York, NY 10065, USA; Department of Biology and Biotechnology 'Charles Darwin', Sapienza University of Rome, Rome 00185, - - 2014
MicroRNAs (miRNAs) belonging to the evolutionary conserved miR-302 family play important functions in Embryonic Stem Cells (ESCs). The expression of some members, such as the human miR-302 and mouse miR-290 clusters, is regulated by ESC core transcription factors. However, whether miRNAs act downstream of signaling pathways involved in human ESC ...
Lu Lu L Tulane University School of - - 2014
MicroRNAs (miRs) are a group of small, non-coding RNAs that modulate the translation of genes by binding to specific target sites in the target mRNA. This study investigated the biological function and molecular mechanism of microRNA-21 (miR-21) in human cholangiocarcinoma. In situ hybridization analysis of human cholangiocarcinoma specimens showed increased ...
Bomer Nils N Department of Molecular Epidemiology, LUMC, Leiden, The - - 2014
To investigate how the genetic susceptibility gene DIO2 confers risk to osteoarthritis (OA) onset in humans and to explore whether counteracting the deleterious effect could contribute to novel therapeutic approaches. Epigenetically regulated expression of DIO2 was explored by assessing methylation of positional CpG-dinucleotides and the respective DIO2 expression in OA-affected ...
Maciejewska I I Department of Dental Prosthodontics, Medical University of Gdansk, 80-210 Gdansk, - - 2014
TWIST1 plays a crucial role in dentinogenesis, and its activity depends on both a dimerization partner selection and phosphorylation. Other factors, like Id proteins, can affect the availability of dimerization partners for TWIST1, subsequently leading to diverse biological outcomes. The purpose of this study was to evaluate an impact of ...
Chu Xiufeng X Department of Microbiology & Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, - - 2014
Fbx4 is a specific substrate recognition component of SCF ubiquitin ligases that catalyzes the ubiquitination and subsequent degradation of cyclin D1 and Trx1. Two isoforms of human Fbx4 protein, the full length Fbx4α and the C-terminal truncated Fbx4β have been identified, but their functions remain elusive. In this study, we ...
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