Search Results
Results 201 - 250 of 3327
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Kämpfer N N Institute of Pharmacology & Toxicology, University of Bonn, Biomedical Center, Sigmund-Freud-Str. 25, D-53105, Bonn, - - 2014
Based on their bronchodilatory effect, β2-adrenoceptor agonists constitute essential elements in the treatment of bronchial asthma and COPD. As treatment with β2-adrenoceptor agonists has been associated with worsening of airway hyper-reactivity, possibly because of loss of β-adrenoceptor function, molecular mechanism of the regulation of β2-adrenoceptor expression were studied. MRC-5 human ...
Song Qiancheng Q Department of Cell Biology, School of Basic Medical Sciences, Southern Medical - - 2014
The nodal regulatory properties of microRNAs in metastatic cancer may offer new targets for therapeutic control. Here we report that upregulation of miR-483-5p is correlated with the progression of human lung adenocarcinoma. miR-483-5p promotes epithelial-mesenchymal transition (EMT) accompanied by invasive and metastatic properties of lung adenocarcinoma. Mechanistically, miR-483-5p is activated ...
Friedländer Marc R - - 2014
MicroRNAs (miRNAs) are established regulators of development, cell identity and disease. Although nearly two thousand human miRNA genes are known and new ones are continuously discovered, no attempt has been made to gauge the total miRNA content of the human genome. Employing an innovative computational method on massively pooled small ...
Kazantseva Jekaterina J Protobios LLC, Mäealuse 4, Tallinn, 12618, Estonia, - - 2014
Expression of general transcription factor and co-activator TAF4 varies during development and in the processes of cell differentiation with suggested connection to neurodegenerative diseases. Here, we show that expression of TAF4 alternative splice variants is different in various regions of the human brain, substantiating the role of alternative splicing of ...
Rosa Alessandro A Laboratory of Molecular Vertebrate Embryology, The Rockefeller University, New York, NY 10065, USA; Department of Biology and Biotechnology 'Charles Darwin', Sapienza University of Rome, Rome 00185, - - 2014
MicroRNAs (miRNAs) belonging to the evolutionary conserved miR-302 family play important functions in Embryonic Stem Cells (ESCs). The expression of some members, such as the human miR-302 and mouse miR-290 clusters, is regulated by ESC core transcription factors. However, whether miRNAs act downstream of signaling pathways involved in human ESC ...
Lu Lu L Tulane University School of - - 2014
MicroRNAs (miRs) are a group of small, non-coding RNAs that modulate the translation of genes by binding to specific target sites in the target mRNA. This study investigated the biological function and molecular mechanism of microRNA-21 (miR-21) in human cholangiocarcinoma. In situ hybridization analysis of human cholangiocarcinoma specimens showed increased ...
Bomer Nils N Department of Molecular Epidemiology, LUMC, Leiden, The - - 2014
To investigate how the genetic susceptibility gene DIO2 confers risk to osteoarthritis (OA) onset in humans and to explore whether counteracting the deleterious effect could contribute to novel therapeutic approaches. Epigenetically regulated expression of DIO2 was explored by assessing methylation of positional CpG-dinucleotides and the respective DIO2 expression in OA-affected ...
Maciejewska I I Department of Dental Prosthodontics, Medical University of Gdansk, 80-210 Gdansk, - - 2014
TWIST1 plays a crucial role in dentinogenesis, and its activity depends on both a dimerization partner selection and phosphorylation. Other factors, like Id proteins, can affect the availability of dimerization partners for TWIST1, subsequently leading to diverse biological outcomes. The purpose of this study was to evaluate an impact of ...
Chu Xiufeng X Department of Microbiology & Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, - - 2014
Fbx4 is a specific substrate recognition component of SCF ubiquitin ligases that catalyzes the ubiquitination and subsequent degradation of cyclin D1 and Trx1. Two isoforms of human Fbx4 protein, the full length Fbx4α and the C-terminal truncated Fbx4β have been identified, but their functions remain elusive. In this study, we ...
Su Shengzhong S Center for Molecular Toxicology & Carcinogenesis, The Pennsylvania State University, 101 Life Sciences Bldg, University Park, PA - - 2014
In humans, microsomal epoxide hydrolase (mEH) contributes important biological functions that underlie both detoxification and bioactivation fates arising from exposures to foreign chemicals. Previously, we discovered that human mEH gene transcription is initiated from alternative promoters. The respective transcripts are programmed with tissue specificity and the upstream E1b promoter contributes ...
Olgun N S NS Women and Children's Research Laboratory, Winthrop University Hospital, Mineola, NY 11501, USA. Electronic address: - - 2014
The pro-inflammatory mediator and potent vasoconstrictor Endothelin-1 (ET-1) is known to be expressed in the placenta. We have recently demonstrated that very low, non-toxic doses of carbon monoxide (CO), prevented infection-induced preterm birth in mice. However the effect(s) of CO on human gestational tissues is yet to be fully explored. ...
Gomes Carolina Cavaliéri - - 2014
STAG2 depletion leads to loss of centromere cohesion in vitro, and some human neoplasms have been shown to lose expression of this protein. As a result, STAG2 loss has been shown to cause chromosomal instability and aneuploidy in human cancer cell lines. We tested the hypothesis that aneuploid salivary gland ...
Robinett Kathryn S KS 1 Department of Medicine, Division of Pulmonary and Critical Care Medicine, University of Maryland School of Medicine, Baltimore, - - 2014
Bitter taste receptors (TAS2Rs) have recently been found to be expressed on human airway smooth muscle (HASM), and their activation results in marked relaxation. These agents have been proposed as a new class of bronchodilators in the treatment of obstructive lung diseases because they act via a different mechanism than ...
Olsen Keith C KC 1 Department of Microbiology and - - 2014
Idiopathic pulmonary fibrosis (IPF) is characterized by progressive fibrotic destruction of normal lung architecture. Due to a lack of effective treatment options, new treatment approaches are needed. We previously identified transglutaminase (TG)2, a multifunctional protein expressed by human lung fibroblasts (HLFs), as a positive driver of fibrosis. TG2 catalyzes crosslinking ...
Heidemann Jan J Department of Internal Medicine B, University of Münster, Albert-Schweitzer-Campus 1, 48149, Münster, - - 2014
Leukocyte-specific transcript 1 (LST1) encoded peptides are involved in immunomodulation and nanotube-mediated cell-cell communication. The aim of this study was to assess the expression of LST1 in colonic epithelium and endothelium during intestinal inflammation. LST1 expression was evaluated by RT-PCR, FACS, western blot analysis, and immunohistochemistry in intestinal epithelial Caco-2 ...
Liu Wenlei W Department of Oral and Maxillofacial Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, - - 2014
Temsirolimus acts as a mammalian target of rapamycin (mTOR)-dependent autophagic inhibitor. In order to clarify its effects and mechanisms on human salivary adenoid cystic carcinoma (ACC), we examined whether temsirolimus induced autophagy as the mTOR inhibitor in ACC, both in vitro and in vivo. In this study, MTT assay showed ...
Aenlle Kristina K KK GRECC and Research Service, Veterans Affairs Medica l Center, Miami, FL, 33125, USA; Departments - - 2014
Hepatocyte growth factor (HGF) is a paracrine factor involved in organogenesis, tissue repair and wound healing. We report here that HGF promotes osteogenic differentiation through the transcription of key osteogenic markers, including Osteocalcin, Osterix, and Osteoprotegerin in human mesenchymal stem cells and is a necessary component for the establishment of ...
Shi Sandra S NorthShore University HealthSystem/University of Chicago, United - - 2014
The human dopamine receptor D2 (DRD2) has been implicated in the pathophysiology of schizophrenia (SZ) and other neuropsychiatric disorders. Most antipsychotic drugs influence dopaminergic transmission through blocking dopamine receptors, primarily DRD2. We report here the post-transcriptional regulation of DRD2 expression by two brain-expressed microRNAs (miRs), miR-326 and miR-9, in an ...
Oustric Vincent V Institut National de la Santé et de la Recherche Médicale, U1149, Centre de Recherches sur l'Inflammation, F-75018 Paris, - - 2014
In 90% of people with erythropoietic protoporphyria (EPP), the disease results from the inheritance of a common hypomorphic FECH allele, encoding ferrochelatase, in trans to a private deleterious FECH mutation. The activity of the resulting FECH enzyme falls below the critical threshold of 35%, leading to the accumulation of free ...
Zhao Yang Y Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh; Magee Womens Research - - 2014
The aim of this study was to examine the effects of 17-alpha hydroxyprogesterone caproate (17-OHPC) on the activity and expression of several common hepatic cytochrome P450 enzymes. Primary human hepatocytes were pre-treated with vehicle or 17-OHPC (0.1 and 1 μM) for 72 hours, then incubated for 1 hour with a ...
Kretschmer A A Research Unit of Molecular Epidemiology, Helmholtz Zentrum München, Neuherberg, - - 2014
Type 2 immune responses directed by Th2 cells and characterized by the signature cytokines IL4, IL5, and IL13 play major pathogenic roles in atopic diseases. Single nucleotide polymorphisms in the human Th2 cytokine locus in particular in a locus control region within the DNA repair gene RAD50, containing several RAD50 ...
Yu Jing J Department of Geratology, the First Hospital Affiliated to Nanjing Medical University, Nanjing, People's Republic of - - 2014
Clinical evidence shows that visceral fat accumulation decreases while subcutaneous fat increases in patients treated with thiazolidinediones (TZDs), a type of PPARγ agonists. To clarify the molecular mechanism of the differential effects of PPARγ agonists on subcutaneous and visceral adipose, we investigated expression profiling of PPARγ-regulated miRNAs using miRNA microarray. ...
Lapierre Marion - - 2014
Deregulation of the Wnt/APC/β-catenin signaling pathway is an important consequence of tumor suppressor APC dysfunction. Genetic and molecular data have established that disruption of this pathway contributes to the development of colorectal cancer. Here, we demonstrate that the transcriptional coregulator RIP140 regulates intestinal homeostasis and tumorigenesis. Using Rip140-null mice and ...
Wu Zhouwei Z Department of Dermatology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, China; Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, - - 2014
Aquaporin 3 (AQP3) is the predominant aquaporin in the skin and is overexpressed in hyperplastic epidermal disorders. Upregulation of AQP3 contributes to keratinocyte proliferation and epidermal hyperplasia. Resveratrol, a natural polyphenol, has an anti-proliferative effect on normal human epidermal keratinocytes (NHEKs), but its exact mechanism remains largely unknown. To investigate ...
Permatasari Felicia F Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, - - 2014
This study was aimed to evaluate the anti-photoaging effects of Botulinum Toxin Type A (BoNTA) in Ultraviolet B-induced premature senescence (UVB-SIPS) of human dermal fibroblasts (HDFs) in vitro and the underlying mechanism. We established a stress-induced premature senescence model by repeated subcytotoxic exposures to Ultraviolet B (UVB) irradiation. The aging ...
Park Jeong Hyeon JH Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand. Electronic address: - - 2014
p53 and NF-κB are key transcription factors in regulating the gene expression program of cellular and organismal senescence. PPM1B is a member of the protein phosphatase 2C family and plays a role in negatively regulating p53 and NF-κB thereby possibly attenuating the gene expression program of cellular senescence. Here, possible ...
Malaisse Jérémy J Research Unit of Molecular Physiology (URPHYM), NARILIS, University of Namur, Namur, - - 2014
Hyaluronan (HA) is a glycosaminoglycan synthesized directly into the extracellular matrix by three hyaluronan synthases (HAS1, HAS2, and HAS3). HA is abundantly synthesized by keratinocytes but its epidermal functions remain unclear. We used culture models to grow human keratinocytes as autocrine monolayers or as reconstructed human epidermis (RHE) to assess ...
Guo Chunming C Center for Pregnancy and Newborn Research (CMG, LM, KS), Dept. OB/GYN, University of Texas Health Science Center San Antonio, San Antonio, TX 78229,USA.; School of Life Sciences (WSW, CL), Fudan University, Shanghai 200433, P.R.China; Center for Reproductive Medicine (KS), Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200135, - - 2014
Abundant evidence indicates a pivotal role of prostaglandin F2α (PGF2α) in human parturition. Both the fetal and maternal sides of the fetal membranes synthesize PGF2α. In addition to the synthesis of PGF2α from PGH2 by PGF synthase (PGFS), PGF2α can also be converted from PGE2 by carbonyl reductase 1 (CBR1). ...
Ren J-G JG The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, - - 2014
Previous studies have implicated vascular destabilization and changes of extracellular matrix (ECM) composition in venous malformations (VMs). To evaluate the expression levels of CCN family of matricellular proteins in VMs, and meanwhile explore their potential association with the vascular destabilization. The expression levels of CCN1-6, TGF-β, p-Tie2 and p-PDGFRβ in ...
Lillehoj Erik P EP 1University of Maryland - - 2014
Sialic acids on glycoconjugates play a pivotal role in many biological processes. In the airways, sialylated glycoproteins and glycolipids are strategically positioned on the plasma membranes of epithelia to regulate receptor-ligand, cell-cell, and host-pathogen interactions at the molecular level. We now demonstrate, for the first time, sialidase activity for ganglioside ...
Zhou Hualin H Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China, - - 2014
Emerging studies support that RNA-binding proteins (RBPs) play critical roles in human biology and pathogenesis. RBPs are essential players in RNA processing and metabolism, including pre-mRNA splicing, polyadenylation, transport, surveillance, mRNA localization, mRNA stability control, translational control and editing of various types of RNAs. Aberrant expression of and mutations in ...
Wang Bingmei B Fujian Key Laboratory of Developmental and Neuro Biology, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian, 350108, People's Republic of China, - - 2014
The WNT/β-CATENIN signaling has been demonstrated to play critical roles in mouse tooth development, but little is known about the status of these molecules in human embryonic tooth. In this study, expression patterns of WNT/β-CATENIN signaling components, including WNT ligands (WNT3, WNT5A), receptors (FZD4, FZD6, LRP5), transducers (β-CATENIN), transcription factors ...
Kessler Almuth Friederike - - 2014
The human endogenous retrovirus K (HERV-K) has been acquired by the genome of human ancestors million years ago. It is the most complete of the HERVs with transcriptionally active gag, pol and env genes. Splice variants of env, which are rec, 1.5 kb transcript and Np9 have been suggested to ...
Tantra Martesa M Clinical Neuroscience, Max Planck Institute of Experimental Medicine, Göttingen, - - 2014
The X-chromosomal MECP2/Mecp2 gene encodes methyl-CpG-binding protein 2, a transcriptional activator and repressor regulating many other genes. We discovered in male FVB/N mice that mild (~50%) transgenic overexpression of Mecp2 enhances aggression. Surprisingly, when the same transgene was expressed in C57BL/6N mice, transgenics showed reduced aggression and social interaction. This ...
Brinzevich Daria D Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, - - 2014
Human endogenous retroviruses (HERVs) are viruses that have colonized the germ line and spread through vertical passage. Only the more recently acquired HERVs, such as the HERV-K (HML-2) group, maintain coding open reading frames. Expression of HERV-Ks has been linked to different pathological conditions, including HIV infection, but our knowledge ...
Wang Zhican Z Department of Pharmaceutics, University of Washington, Seattle, WA, United - - 2014
25-hydroxyvitamin D3 (25OHD3) is used as a clinical biomarker for assessment of vitamin D status. Blood levels of 25OHD3 represent a balance between its formation rate and clearance by several oxidative and conjugative processes. In the present study, the identity of human UDP-glucuronyltransferases (UGTs) capable of catalyzing the 25OHD3 glucuronidation ...
Fusi Camilla C Department of Health Sciences, Clinical Pharmacology and Oncology Unit, University of Florence, Florence, - - 2014
A subgroup of the transient receptor potential (TRP) channels including vanilloid 1 (TRPV1), TRPV2, TRPV3, TRPV4, and TRP ankyrin 1 (TRPA1), is expressed in cutaneous peptidergic somatosensory neurons, and has been found in skin non-neuronal cells, such as keratinocytes. Different cancer cells expressed TRPs, where they may exert either pro- ...
Bartlett Marian Stewart MS Institute for Neural Computation, University of California, 9500 Gilman Drive, MC 0440, La Jolla, San Diego, CA 92093-0440, USA. Electronic address: - - 2014
In highly social species such as humans, faces have evolved to convey rich information for social interaction, including expressions of emotions and pain [1-3]. Two motor pathways control facial movement [4-7]: a subcortical extrapyramidal motor system drives spontaneous facial expressions of felt emotions, and a cortical pyramidal motor system controls ...
Javam M M Department of Physiology, University of Toronto, Toronto, - - 2014
The placenta contains efflux transporters, including P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP), that limit the passage of xenobiotics, certain hormones and nutrients from the maternal to the fetal circulation. The expression of these transporters changes with gestational age, yet the mechanisms involved remain unknown. However, the changes in ...
Surmon Laura - - 2014
Preeclampsia is a complication of pregnancy characterised by gestational hypertension and proteinuria and is a leading cause of morbidity and mortality in both mothers and infants. Certain anti-angiogenic factors have long been implicated in the pathogenesis of preeclampsia and the placental expression of factors such as soluble fms-like tyrosine kinase-1 ...
Chen Jin - - 2014
Fibronectin (FN) is commonly used in the development of serum-free media for the expansion of mesenchymal stem cells (MSCs). This study was aimed to observe if thrombin could stimulate FN secretion by human bone marrow MSCs and investigate the potential underlying mechanisms. PCR was performed to detect the expression of ...
Pekala Kelly R KR Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, - - 2014
Normal pancreatic epithelium progresses through various stages of pancreatic intraepithelial neoplasms (PanINs) in the development of pancreatic ductal adenocarcinoma (PDAC). Transcriptional regulation of this progression is poorly understood. In mouse, the hepatic nuclear factor 6 (Hnf6) transcription factor is expressed in ductal cells and at lower levels in acinar cells ...
Reyes-Gordillo Karina K Lipid Research Laboratory, Veterans Affairs Medical Center, Washington, District of Columbia; Department of Biochemistry and Molecular Medicine, George Washington University Medical Center, Washington, District of - - 2014
Alcohol-induced liver fibrosis and eventually cirrhosis is a leading cause of death. Acetaldehyde, the first metabolite of ethanol, up-regulates expression of the human α2(I) collagen gene (COL1A2). Early acetaldehyde-mediated effects involve phosphorylation and nuclear translocation of SMAD3/4-containing complexes that bind to COL1A2 promoter to induce fibrogenesis. We used human and ...
Merhi Zaher Z Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, University of Vermont College of Medicine, Burlington, VT, - - 2014
Context: Vitamin D deficiency is common among reproductive-aged women and has a role in female reproduction. Objective: This study evaluated the role of 1,25-dihydroxyvitamin D3 (vit D3) in ovarian follicular development and steroidogenesis by using a human granulosa cell (GC) model. Design, setting and participants: 54 women who underwent IVF ...
Tsai Steven C SC The Saban Research Institute of Children's Hospital Los - - 2014
Our knowledge of the molecular mechanisms underlying human embryonic stem cell (hESC) self-renewal and differentiation is incomplete. Oct4 is a critical regulator of pluripotency whose level is precisely controlled in mouse embryonic stem cells (mESC). However, studies of human OCT4 are often confounded by the presence of three isoforms and ...
Li J-P JP Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China; Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, - - 2014
Lipopolysaccharide (LPS)-mediated signaling in host cells involves Toll-like receptor 4 (TLR4) accessory molecules, including LPS-binding protein (LBP), cluster of differentiation 14 (CD14) and lymphocyte antigen 96 (MD-2). However, expression of these innate defense molecules in various compartments of the human periodontium is unclear. The aim of this study was to ...
Rossi Antonio A Institute of Translational Pharmacology, CNR, - - 2014
The zinc-finger AN1-type domain 2a gene, also known as AIRAP, was recently identified as a novel human canonical heat shock gene, strictly controlled by heat shock factor-1 (HSF1). Little is known about AIRAP gene regulation in human cells. Herein we report that bortezomib, a proteasome inhibitor with anticancer and antiangiogenic ...
Jurado Ashley R - - 2014
Messenger RNA precursors (pre-mRNAs) are produced as the nascent transcripts of RNA polymerase II (Pol II) in eukaryotes and must undergo extensive maturational processing, including 5´-end capping, splicing and 3´-end cleavage and polyadenylation. This review will summarize the structural and functional information reported over the past few years on the ...
El Husseini Diala D Laboratoire d'Études Moléculaires des Valvulopathies (LEMV), Groupe de Recherche en Valvulopathies (GRV), Quebec Heart and Lung Institute/Research Center, Department of Surgery, Laval University, Quebec, - - 2014
Calcific aortic valve disease (CAVD) is a disorder characterized by an abnormal mineralization, which may have intricate links with inflammation. Interleukin-6 (IL-6) and its cognate cytokines are widely expressed and exert pleiotropic effects on different tissues. In this study, we examined the expression of the IL-6 family of cytokines in ...
Skalniak Anna A Laboratory of Molecular Genetics and Virology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Kraków, - - 2014
The importance of monocyte chemotactic protein-1-induced protein 1 (MCPIP1) in the negative regulation of inflammatory reactions has already been extensively studied. However, its role in cancer is not yet established. We studied MCPIP1 gene expression in primary human neuroblastomas and several neuroblastoma cell lines. Our results showed a lack of ...
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