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Giovannoni Isabella I Dep. Pathology, Children's Hospital Bambino Gesù, IRCCS, Rome, - - 2014
Homozygous individuals with alpha-1-antitrypsin deficiency (AATD) type PiZ have an increased risk of chronic liver disease and hepatocellular carcinoma (HCC). It is noteworthy that HCCs are composed by hepatocytes without accumulation of AAT, but the reason for this remains unclear. The aim of this study was to determine liver pathology ...
Bae Byoung-Il BI Division of Genetics and Genomics, Manton Center for Orphan Disease, and Howard Hughes Medical Institute, Boston Children's Hospital, Broad Institute of MIT and Harvard, and Departments of Pediatrics and Neurology, Harvard Medical School, Boston, MA 02115, - - 2014
The human neocortex has numerous specialized functional areas whose formation is poorly understood. Here, we describe a 15-base pair deletion mutation in a regulatory element of GPR56 that selectively disrupts human cortex surrounding the Sylvian fissure bilaterally including "Broca's area," the primary language area, by disrupting regional GPR56 expression and ...
Kallen Amanda N AN 1Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, - - 2014
Objective:The mammalian cytoskeleton is composed in part from keratin filaments which form a complex, highly dynamic intracellular network. We investigate the expression of cytokeratin 15 (CK15) in human endometrium and its regulation by HOXA10 in the human endometrial cell lines.Methods:Endometrial biopsies from throughout the menstrual cycle (N = 32) were ...
Celardo Ivana I Medical Research Council, Toxicology Unit, Leicester University, Leicester LE1 9HN, - - 2014
p63 is a transcriptional factor belonging to p53 family of genes. Beside the role in cancer, partially shared with p53 and the other member p73, p63 also plays exclusive roles in development and homeostasis of ectodermal/epidermal-related organs. Here we show that p63 transcriptionally controls the expression of the matrix metallopeptidase ...
Elain Gaelle G Autoimmunity, Transplantation and Inflammatory Disease, Novartis Institutes for BioMedical Research, Novartis Pharma AG, Basel, - - 2014
The family of interleukin 17 receptors (IL17Rs), subtypes IL17RA-IL17RE, is targeted by the group of pro-inflammatory IL17 cytokines (IL17A-F) and moreover the newly developed anti-IL17A antibody secukinumab (AIN457) has shown promise in Phase II trials in multiple sclerosis. Here, we show that human astrocytes, isolated from a fetal cerebral cortex, ...
Srinivasa Babu K K 1Centre for Biotechnology, Anna University, Chennai, India; and 2Centre for Bioseparation Technology, VIT University, Vellore, - - 2014
Human granulocyte-macrophage colony-stimulating factor (hGMCSF) is a proinflammatory cytokine and hematopoietic growth factor. Recombinant human granulocyte-macrophage colony-stimulating factor (rhGMCSF) serves as a biotherapeutic agent in bone marrow stimulations, vaccine development, gene therapy approaches, and stem cell mobilization. The objective of the present study includes construction of rhGMCSF having N-terminal intein ...
Feng Chenzhuo C Department of Anesthesiology, University of Virginia, Charlottesville, VA, USA (C.F., J.Y., J.L., Z.Z.); Department of Microbiology, Immunology and Cancer Biology, University of Virginia, Charlottesville, VA, USA (Y.Z., R.A.); Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China (J.Y.); Department of Anesthesiology, Fourth Affiliated Hospital, Harbin Medical University, Harbin, China - - 2014
The biological functions of regulatory factor (RF)X1, a transcription factor, are not known. Since the RFX1 gene is often epigenetically silenced and clusters of differentiation (CD)44 proteins that regulate cancer cell biology are increased in human glioblastomas, we designed this study to determine whether RFX1 could regulate CD44 expression in ...
Boles Nathan C NC The Neural Stem Cell Institute, 1 Discovery Drive, Rensselaer, NY 12144, - - 2014
Neural induction is the first fundamental step in nervous system formation. During development, a tightly regulated niche modulates transient extracellular signals to influence neural lineage commitment. To date, however, the cascade of molecular events that sustain these signals in humans is not well understood. Here we show that NPTX1, a ...
Wei Jun J Department of Neurosurgery, China-Japan Union Hospital, Jilin University, Changchun, 130033, - - 2014
RNA editing is catalyzed by adenosine deaminases acting on RNA (ADARs). ADAR2 is the main enzyme responsible for recoding editing in humans. Adenosine-to-inosine (A-to-I) editing at the Q/R site is reported to be decreased in gliomas; however, the expression of ADAR2 mRNA was not greatly affected. We determined ADAR2 mRNA ...
Furihata Tomomi T Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, Chiba - - 2014
  Human equilibrative nucleoside transporter 1 (hENT1) transports various nucleoside analogues into cells. Although the single hENT1 promoter region (P1) and the mRNA isoform (a1) have been characterized previously, we have recently identified additional promoter regions P2 and P3 (which primarily generate c1/2/3 mRNAs and d1/2/3/4 mRNAs, respectively) in the human ...
Peavey Mary M 1Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC, - - 2014
Objective:Integrin α11β1 is a collagen receptor specific to fibroblasts that regulates myofibroblast differentiation. We sought to determine whether α11β1 is expressed in myometrium and fibroids and whether tissue expression varies.Design:Comparison of α11 in human myometrium and fibroids using Western blot and RNA in situ hybridization.Materials and Methods:Specimens were obtained from ...
Cheng Shin-Ei - - 2014
Up-regulation of intercellular adhesion molecule-1 (ICAM-1) is frequently implicated in lung inflammation and lung diseases, such as idiopathic pulmonary fibrosis (IPF). Thrombin has been shown to play a key role in inflammation via adhesion molecules induction, and then causes lung injury. However, the mechanisms underlying thrombin-induced ICAM-1 expression in human ...
Markmiller Sebastian S Ludwig Institute for Cancer Research, Melbourne-Parkville Branch, Parkville, VIC 3050, - - 2014
Minor class or U12-type splicing is a highly conserved process required to remove a minute fraction of introns from human pre-mRNAs. Defects in this splicing pathway have recently been linked to human disease, including a severe developmental disorder encompassing brain and skeletal abnormalities known as Taybi-Linder syndrome or microcephalic osteodysplastic ...
Chang Chung-Te CT Department of Biochemistry, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, - - 2014
The removal of the 5'-cap structure by the decapping enzyme DCP2 and its coactivator DCP1 shuts down translation and exposes the mRNA to 5'-to-3' exonucleolytic degradation by XRN1. Although yeast DCP1 and DCP2 directly interact, an additional factor, EDC4, promotes DCP1-DCP2 association in metazoan. Here, we elucidate how the human ...
Liu Yichuan Y From the Departments of Biostatistics and Epidemiology and Biology, Cardiovascular Institute, Perelman School of Medicine and School of Arts and Science at the University of Pennsylvania, - - 2014
Inappropriate transcriptional activation of innate immunity is a pathological feature of several cardiometabolic disorders, but little is known about inflammatory modulation of long intergenic noncoding RNAs (lincRNAs) in disease-relevant human tissues. We applied deep RNA sequencing (>500 million filtered reads per sample) to blood and adipose during low-dose experimental endotoxemia ...
Wang Lu L State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, - - 2014
We have previously reported that elevated expression of sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase 2 (SERCA2) was related to the malignant degree of different types of human liposarcoma. Here, we investigated the effects of high SERCA2b expression on proliferation and differentiation of preadipocyte-like human liposarcoma cell line SW872 cells. SW872 cells were stably ...
Sánchez-Hernández Miriam M Centro Oceanográfico de Murcia, Instituto Español de Oceanografía (IEO), Carretera de la Azohía s/n. Puerto de Mazarrón, 30860 Murcia, - - 2014
In humans, alternative splicing of androgen receptor (AR) is usually involved in some diseases. However, our knowledge about the presence of AR variants in other species and its importance for immunity is scant. Here, we report the identification of a constitutively active AR variant lacking the ligand-binding domain (LBD), ARΔLBD, ...
Yammani Raghunatha R RR Section of Molecular Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, - - 2014
Objective: Nuclear protein-1 (nupr1) is a stress-inducible protein involved in gene transcription. The objectives of this study were to determine (a) if chondrocytes express nupr1 and (b) whether nupr1 regulates MMP-13 expression. Methods: Paraffin-embedded cartilage sections from normal human and osteoarthritic cartilage were immunostained using anti-nupr1 antibody. Total RNA to ...
Cizkova Katerina K Department of Histology and Embryology; Palacky University; Olomouc, Czech - - 2014
There is growing evidence that some members of cytochrome P450 enzymes contribute to regulation of normal prenatal development. CYP epoxygenases (CYP2C and CYP2J subfamilies) convert arachidonic acid into four regioisomeric eicosatrienoic acids (EETs), biologically active molecules involved in mitogenesis and cell signaling. Almost nothing is known about localization of their ...
Mehus Aaron A AA University of North Dakota, United - - 2014
Human metallothioneins (MTs) are important regulators of metal homeostasis and protectors against oxidative damage. Their altered mRNA expression has been correlated with metal toxicity and a variety of cancers. Current immunodetection methods lack the specificity to distinguish all twelve human isoforms. Each, however, can be distinguished by the masses of ...
Li Wei W Yangzhou - - 2014
Sulfotransferase (SULT) 2A1 catalyzes sulfonation of drugs and endogenous compounds, and plays an important role in xenobiotic metabolism as well as in the maintenance of steroid and lipid homeostasis. A recent study showed that 17β-estradiol (E2) increases the mRNA levels of SULT2A1 in human hepatocytes. Here we report the underlying ...
Morató Roser R Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, 17071, Girona, - - 2014
One of the major obstacles for the vitrification of mature porcine oocytes with ethylene glycol is their low permeability to this cryoprotectant, which results in osmotic stress-induced cell damage and low survival. Pig blastocysts, on the other hand, show enhanced water and cryoprotectant permeability, which has been related to the ...
Na Hee Sam HS Department of Oral Microbiology, Pusan National University, Yangsan, 626-810, Republic of - - 2014
In the gingival tissues of patients with periodontitis, inflammatory responses are mediated by a wide variety of genes. In our previous screening study, plasminogen activator inhibitor type 1 (PAI-1) mRNA binding protein expression was increased in gingiva from periodontitis patients. In this study, we further investigated the signaling pathway involved ...
Zaga-Clavellina Veronica V Department of Cell Biology, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, - - 2014
To test the effect of PRL on expression of proinflammatory cytokines and MMP-9 in vitro. Tissue explants were incubated from 4 to 48 h alone or in the presence of 500 ng/mL PRL, and mRNA expression in tissues and secretion of IL-1β, TNF-α, MMP-2 and MMP-9 was quantified. Fetal membranes ...
Vos Maria Caroline - - 2014
The aim of this study was to investigate the presence of MMP-14 and MMP-2 during human ovarian follicular development using immunohistochemistry, and the activity of MMP-2 in follicular fluid using zymography. Ovarian tissue collected from the archives of the Department of Pathology was examined and medical records and histopathology were ...
Jangani Maryam M University of Manchester, United - - 2014
Glucocorticoids (Gc) regulate cell fate and immune function. We identified the metasasis-promoting methyltransferase, metastasis-related methyltransferase 1 (WBSCR22/Merm1) as a novel glucocorticoid receptor (GR) regulator, relevant to human disease. Merm1 binds the GR co-activator GRIP1, but not GR. Loss of Merm1 impaired both GR transactivation, and transrepression, by reducing GR recruitment ...
Lyros Orestis O Medical College of - - 2014
Squamous esophageal epithelium adapts to acid reflux-mediated injury by proliferation and differentiation. Induction of the Wnt-antagonist, Dickkopf-1 (Dkk1) is involved in tissue repair during inflammation and cellular injury. In this study, we aimed to identify the biological role of Dkk1 in human reflux-esophagitis with respect to cell growth and regulation ...
Weng Chih-Ming CM Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, - - 2014
Endothelin-1 (ET-1) acts as a key mediator of vasoconstriction and tissue repair. Overproduction of connective tissue growth factor (CTGF) underlies the development of lung fibrosis. ET-1 induces expression of matrix-associated genes in lung fibroblasts, however, little is known about the signaling pathway of CTGF expression caused by ET-1. In this ...
Jung Hyejung H Department of Life Sciences, Division of Life and Pharmaceutical Sciences and the Center for Cellular Homeostasis, Ewha Womans University, Seoul, 120-750, - - 2014
Syndecan-2, a transmembrane heparan sulfate proteoglycan that is highly expressed in melanoma cells, regulates melanoma cell functions (e.g., migration). Since melanoma is a malignant tumor of melanocytes, which largely function to synthesize melanin, we investigated the possible involvement of syndecan-2 in melanogenesis. Syndecan-2 expression was increased in human skin melanoma ...
Davidson Lee L Wellcome Trust Centre for Cell Biology, Edinburgh EH9 3JR, United - - 2014
3' end formation of pre-mRNAs is coupled to their transcription via the C-terminal domain (CTD) of RNA polymerase II (Pol II). Nearly all protein-coding transcripts are matured by cleavage and polyadenylation (CPA), which is frequently misregulated in disease. Understanding how transcription is coordinated with CPA in human cells is therefore ...
Wang Ke K Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, Jiangsu Province, China. Electronic address: - - 2014
Ciliary neurotrophic factor (CNTF) is characterized as a neuropoietic cytokine for a broad spectrum of neurons, leading to its evaluation in humans suffering from neurodegenerative diseases. Due to its wide range of biological applications, high yield production of soluble biologically active recombinant human CNTF (rhCNTF) in heterologous expression system is ...
Ohata Yasuhisa Y Department of Bone and Mineral Research, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka, 594-1101, Japan; Department of Pediatrics, Osaka University Graduate School of Medicine, Suita, Osaka, 565-0871, - - 2014
Fibroblast growth factor 23 (FGF23) functions in an endocrine fashion and requires α-Klotho to exert its effects on the target organs. We have recently demonstrated that the human placenta also expresses α-Klotho, which led us to hypothesize that FGF23 may exert effects on the placenta. Immunohistochemical analysis demonstrated the expression ...
Han Limin L Peking University Research Center on Aging, Peking University Health Science Center, Beijing, 100191, People's Republic of China; Department of Biochemistry & Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, People's Republic of - - 2014
SIRT1 is a key protein in mediating the benefits of calorie restriction (CR) in mammals. However, the molecular mechanisms of CR-induced SIRT1 up-regulation in mammals remain unclear. We show that the elevated SIRT1 level is not attributed to the increased SIRT1 mRNA levels, but the enhanced SIRT1 protein stability via ...
Haseeb Abdul A Northeast Ohio Medical University, United - - 2014
5-hydroxymethylcytosine (5-hmC) generated by ten-eleven translocation 1-3 (TET1-3) enzymes is an epigenetic mark present in many tissues with different degrees of abundance. IL-1β and TNF-α are the two major cytokines present in arthritic joints that modulate the expression of many genes associated with cartilage degradation in osteoarthritis (OA). In the ...
de Lucas Susana S Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología (CSIC), C/Darwin 3, Campus Cantoblanco, 28049 Madrid, Spain, Ciber de Enfermedades Respiratorias (ISCIII), Mallorca, Spain, Servicio de Genómica Computacional, Centro Nacional de Biotecnología (CSIC), C/Darwin 3, Campus Cantoblanco, 28049 Madrid, Spain and Bioinformática de Sistemas, Centro Nacional de Biotecnología (CSIC), C/Darwin 3, Campus Cantoblanco, 28049 Madrid, - - 2014
Cellular messenger RNAs (mRNAs) are associated to proteins in the form of ribonucleoprotein particles. The double-stranded RNA-binding (DRB) proteins play important roles in mRNA synthesis, modification, activity and decay. Staufen is a DRB protein involved in the localized translation of specific mRNAs during Drosophila early development. The human Staufen1 (hStau1) ...
Scotece Morena M SERGAS, Research Laboratory 9 (NEIRID LAB: Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases), Institute of Medical Research (IDIS), Santiago University Clinical Hospital, Santiago de Compostela, 15706, - - 2014
Nesfatin-1 is a recently discovered satiety-inducing adipokine identified in hypothalamic regions that regulates energy balance. So far, no data exist on NUCB2/nesfatin-1 localization in human and murine chondrocytes. Here, we therefore investigated NUCB2/nesfatin-1 gene and protein expression in human and murine chondrocytes and the effect of nesfatin-1 on pro-inflammatory cytokines ...
Nakamura Yasuhiro Y Department of Pathology, Tohoku University School of Medicine, Sendai, Japan. Electronic address: - - 2014
Aberrant expression of gonadotropin-releasing hormone receptor (GnRHR) has been reported in human adrenal tissues including aldosterone-producing adenoma (APA). However, the details of its expression and functional role in adrenals are still not clear. In this study, quantitative RT-PCR analysis revealed the mean level of GnRHR mRNA was significantly higher in ...
Zhang Ruifeng R 1Department of Respiratory Medicine, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, - - 2014
We previously observed that transgelin was preferentially expressed in human pulmonary arterial smooth muscle cells (PAMSCs) under hypoxia and that the upregulation of transgelin was independent of hypoxia inducible factor 1α (HIF-1α). Reduced transgelin expression was accompanied by significantly impaired migration ability in vitro. However, the regulation mechanism of transgelin ...
Li Rui R Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan 250012, PR China; Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan 250012, PR - - 2014
Secretory human interleukin 4 (hIL4) is an N-glycosylated pleiotropic cytokine. It is unknown if these N-linked glycans are required and essential for hIL4 protein stability, expression, secretion, and activity in vivo, and hIL4 expressed from Pichia pastoris yeast has not been tested to date. In this study, we successfully expressed ...
Chi J J Division of Cardiovascular Medicine, Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Shimoaizuki, Matusoka, Eiheji-Cho, Fukui, - - 2014
The aim of this study was to determine the influence of high glucose (HG) and interleukin (IL)-1β on human cardiac fibroblast (HCF) functions, and to evaluate the effects of eplerenone in these responses. HCFs were cultured in normal or HG media in the absence or presence of IL-1β and/or eplerenone. ...
Verstraelen Jessica - - 2014
Preclinical studies addressing the transcorneal absorption of ophthalmic drugs are mainly performed using ex vivo animal corneas and in vitro corneal cell culture models, leaving open the question of transferability to humans in an in vivo situation. While passive drug absorption through corneal tissue is well understood, little is known ...
Ludwig Anna Lisa AL Department of Biochemistry and Molecular Medicine, University of California, Davis, School of Medicine, Davis, CA, - - 2014
Large expansions of a CGG-repeat element (>200 repeats; full mutation) in the fragile X mental retardation 1 (FMR1) gene cause fragile X syndrome (FXS), the leading single-gene form of intellectual disability and of autism spectrum disorder. Smaller expansions (55-200 CGG repeats; premutation) result in the neurodegenerative disorder, fragile X-associated tremor/ataxia ...
Helan Martin M Department of Anesthesiology, Mayo Clinic, Rochester, USA; ICRC, International Clinical Research Center, Brno, Czech Republic; Department of Anesthesiology and Intensive Care, St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Brno, Czech - - 2014
Within human pulmonary artery, neurotrophin growth factors [NTs; e.g. brain-derived neurotrophic factor (BDNF)] and their high-affinity receptors (tropomyosin-related kinase; Trk) and low-affinity receptors p75 neurotrophin receptor (p75NTR) have been reported, but their functional role is incompletely understood. We tested the hypothesis that BDNF is produced by human pulmonary artery endothelial ...
Flores Omar O Department of Molecular Genetics & Microbiology and Center for Virology, Duke University Medical Center, Durham, NC 27710, - - 2014
It has previously been assumed that the generally high stability of microRNAs (miRNAs) reflects their tight association with Argonaute (Ago) proteins, essential components of the RNA-induced silencing complex (RISC). However, recent data have suggested that the majority of mature miRNAs are not, in fact, Ago associated. Here, we demonstrate that ...
Tsai Hsiao-Chi HC Department of Life Sciences, National Chung Hsing University, Taichung, - - 2014
Osteosarcoma is the most common primary malignant tumor, and its treatments require more effective therapeutic approaches. Paclitaxel has a broad range of antitumor activities, including apoptosis-inducing effects. However, the majority of tumors in patients with advanced cancer eventually develop chemoresistance. Connective tissue growth factor (CTGF) is a secreted protein that ...
Degoricija Marina M Department of Immunology, School of Medicine, University of Split, Šoltanska 2, 21000, Split, - - 2014
Given that the tumor-promoting inflammation has been previously established in squamous cell carcinoma of the bladder but its contribution to development of urothelial carcinoma (UC) still remains elusive, our aim was to study changes in expression and activity of inflammation-mediating NF-κB and STAT3 transcription factors in human urothelial bladder carcinoma ...
Ebeling Sandra S Pharmaceutical Biology and Biotechnology, Albert-Ludwigs-University Freiburg, Freiburg, - - 2014
Birch bark has a long lasting history as a traditional medicinal remedy to accelerate wound healing. Recently, the efficacy of birch bark preparations has also been proven clinically. As active principle pentacyclic triterpenes are generally accepted. Here, we report a comprehensive study on the underlying molecular mechanisms of the wound ...
Crociani Olivia O Dept of Experimental Pathology and Oncology, University of - - 2014
hERG1 channels are aberrantly expressed in several types of human cancers, where they affect different aspects of cancer cell behaviour. A thorough analysis of the functional role and clinical significance of hERG1 channels in gastric cancer (GC) is still lacking. Experimental design: hERG1 expression was tested in a wide (508 ...
Litzenburger Ulrike M UM Department of Neurooncology, Neurology Clinic and National Center for Tumor Diseases University Hospital of Heidelberg, Heidelberg, - - 2014
Indoleamine-2,3-dioxygenase (IDO) inhibitors have entered clinical trials based on their ability to restore anti-tumor immunity in preclinical studies. However, the mechanisms leading to constitutive expression of IDO in human tumors are largely unknown. Here we analyzed the pathways mediating constitutive IDO expression in human cancer. IDO-positive tumor cells and tissues ...
Mosher Andrea A AA 1Department of Physiology & Pharmacology, Alberta Children's Hospital Research Institute for Child and Maternal Health, Faculty of Medicine, University of Calgary, Calgary, Alberta, - - 2014
Prostaglandins are implicated in the labor process, yet the precise role and regulation of the prostaglandin pathway remains to be elucidated. The first step in the pathway is cleavage of membrane phospholipids by phospholipase A2 (PLA2). Previous work demonstrated upregulation of secretory PLA2 (sPLA2)-IIA with labor in human myometrium, and ...
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