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Törőcsik D D Department of Dermatology, Faculty of Medicine, University of Debrecen, Debrecen, - - 2014
Leptin, the adipocyte-secreted hormone that regulates weight, is known to link lipid metabolism with inflammation in various cell types, however its role in human sebocytes has not yet been investigated. The purpose of the study was to investigate the effects of leptin in human sebaceous gland biology. Ob-Rb expression was ...
Kaushik Ramakant R Principal Scientist, Embryo Biotechnology Lab, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, 132 001, - - 2014
The aim of the present study was construction of mammary gland specific expression vector for high level of human insulin (hINS) expression in transgenic buffalo for therapeutic use. We have constructed mammary gland specific vector containing human insulin gene and there expression efficiency was checked into in vitro cultured buffalo ...
Esteso Gloria G Department of Immunology and Oncology, Centro Nacional de Biotecnología/Consejo Superior de Investigaciones Científicas, Madrid 28049, - - 2014
Proteolytic shedding of ligands for the NK group 2D (NKG2D) receptor is a strategy used by tumors to modulate immune recognition by NK cells and cytotoxic T cells. A number of metalloproteases, especially those of the a disintegrin and metalloprotease (ADAM) family, can mediate NKG2D ligand cleavage and this process ...
Kemp Jacqueline R JR Department of Molecular Cardiology, Cleveland Clinic, Cleveland, OH, USA; Regulatory Biology Graduate Program, Cleveland State University, Cleveland, OH, - - 2014
Improper regulation of signaling in vascular smooth muscle cells (VSMCs) by angiotensin II (AngII) can lead to hypertension, vascular hypertrophy and atherosclerosis. The extent to which the homeostatic levels of the components of signaling networks are regulated through microRNAs (miRNA) modulated by AngII type 1 receptor (AT1R) in VSMCs is ...
Gorst I I Hannover Medical School, Cardiology and Angiology, Hannover, - - 2014
Implementation of PTA as standard treatment for patients with myocardial infarction (MI) reduced the mortality in the early phase. However, morbidity due to adverse cardiac remodelling has increased. In MI-patients, serum concentrations of free sialic acids are significantly elevated. Those are released from glycoconjugates by neuraminidases (Neu). Neu play important ...
Wojtal Kacper Andrzej KA University Hospital Zurich - - 2014
Solute carrier transporters (SLC) mediate the uptake of biologically active compounds in the intestine. Reduced oxygenation (hypoxia) is an important factor influencing intestinal homeostasis. The aim of this study was to investigate the pathophysiological consequences of hypoxia on the expression and function of SLCs in human intestine. Hypoxia was induced ...
Ni Kai K Department of Urology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Institute of Andrology, No145 Middle Shandong Road, 200001, Shanghai, China; Department of Urology, Pediatric Urology and Andrology, Section Molecular Andrology, Justus Liebig University Giessen, Schubertstraβe. 81, 35392, Giessen, - - 2014
To investigate the expression and the exact role of leptin under hypoxic conditions in human testis. Five testes, obtained from the patients undergoing orchiectomy for prostate cancer, were used to construct an in-vitro hypoxic culture system for human testicular tissue. Immunohistochemistry was performed to analyze leptin-protein expression. The leptin, leptin-R ...
Mikhaylova Marina - - 2014
The survival promoting peptide Y-P30 has a variety of neuritogenic and neuroprotective effects in vitro and in vivo. In previous work we reported the expression of Y-P30/dermcidin in maternal peripheral blood mononuclear cells (PBMCs) and the transport of the protein to the fetal brain. In this study we analyzed hormonal ...
Grandjenette Cindy C Laboratoire de Biologie Moléculaire et Cellulaire du Cancer, Hôpital Kirchberg, 9, rue Edward Steichen, L-2540 Luxembourg, - - 2014
Increased proliferation rates as well as resistance to apoptosis are considered major obstacles for the treatment of patients with chronic myelogenous leukemia (CML), thus highlighting the need for novel therapeutic approaches. Since senescence has been recognized as a physiological barrier against tumorigenesis, senescence-based therapy could represent a new strategy against ...
Garcia-Sanz Pablo P Fundacion MD Anderson Cancer Center, - - 2014
Myc expression is deregulated in many human cancers. A yeast two hybrid screen has revealed that the transcriptional repressor Sin3b interacts with Myc protein. Endogenous Myc and Sin3b colocalize and interact in the nuclei of human and rat cells, as assessed by coimmunoprecipitation, immunofluorescence, and proximity ligation assay. The interaction ...
Turroni Francesca F Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, - - 2014
Cell surface pili have recently been found in many different bifidobacterial species, including the infant gut commensal Bifidobacterium bifidum PRL2010. Pili produced by PRL2010 have been shown to be important molecular mediators for bacterial interaction with its human host. However, nothing is known about the modulation of their expression in ...
Pendaries Valérie V 1] UMR5165 CNRS, Toulouse, France [2] U1056 INSERM, Toulouse, France [3] University of Toulouse, Toulouse, - - 2014
Atopic dermatitis is a chronic inflammatory skin disorder characterized by defects in the epidermal barrier and keratinocyte differentiation. The expression of filaggrin, a protein thought to play a major role in the function of the epidermis, is down-regulated. However, the impact of this deficiency on keratinocytes is not really known. ...
Bao Lei L Departments of Dermatology, University of Illinois, Chicago, - - 2014
IL-19 plays an important role in asthma by stimulating Th2 cytokine production. Interestingly, IL-4, a key Th2 cytokine, in turn up-regulates IL-19 expression in bronchial epithelial cells, thus forming a positive feedback loop. In atopic dermatitis (AD), another Th2 disease closely related to asthma, IL-19 is up-regulated in the skin. ...
Cloix Lucie - - 2014
INTELECTIN (ITLN) is an adipokine involved in the regulation of insulin sensitivity, inflammatory and immunity response. Serum ITLN levels are lower in obese, diabetic and polycystic ovary syndrome women as compared to control subjects. ITLN has never been studied in ovarian cells. Here, we identified ITLN1 in human ovarian follicles ...
Schmidt Constanze C Department of Cardiology, University of Heidelberg, Im Neuenheimer Feld 410, D-69120 Heidelberg, - - 2014
Effective treatment of atrial fibrillation (AF) remains an unmet need. Human K2P3.1 (TASK-1) K(+) channels display atrial-specific expression and may serve as novel antiarrhythmic targets. In rodents, inhibition of K2P3.1 causes prolongation of action potentials and QT intervals. We utilized a porcine model to further elucidate the significance of K2P3.1 ...
Du Xiaoling - - 2014
Osteosarcoma is a highly genetically unstable tumor with poor prognosis. We performed microarray-based comparative genomic hybridization (aCGH), transcriptome sequencing (RNA-seq), and pathway analysis to gain a systemic view of the pathway alterations of osteosarcoma. aCGH experiments were carried out on 10 fresh osteosarcoma samples. The output data (Gene Expression Omnibus ...
Paterson Clare - - 2014
OBJECTIVE Neuregulin 1 (NRG1) is a multifunctional neurotrophin that mediates neurodevelopment and schizophrenia risk. The NRG1 gene undergoes extensive alternative splicing, and association of brain NRG1 type IV isoform expression with the schizophrenia-risk polymorphism rs6994992 is a potential mechanism of risk. Novel splice variants of NRG1-IV (NRG1-IVNV), with predicted unique ...
Inoue Takayoshi T T Inoue, Pathology, Tohoku University Graduate School of Medicine, Miyagi, 980-8575, - - 2014
Androgens are well known to influence sebum synthesis and secretion. Various factors related to androgen biosynthesis are expressed in human sebaceous glands. In our present study, immunohistochemical analysis of 43 human skin specimens demonstrated that various androgen producing- and metabolizing-enzymes were functionally localized in sebocytes accumulating lipid droplets and that ...
Beck-Broichsitter Benedicta E BE Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, - - 2014
The majority of patients diagnosed with osteomyelitis of the jaw have severe complaints. Unfortunately, the pathogenesis still remains unclear. Human ß-defensins expressed in epithelial and bone tissues as a part of the innate immunity may be involved in disease development. In this study, we hypothesize that expression levels of human ...
Orbelyan Gerasim A GA Department of Medicine, University of Chicago, Chicago, IL - - 2014
The NKG2 family of NK receptors includes activating and inhibitory members. With the exception of the homodimer-forming NKG2D, NKG2 receptors recognize the nonclassical MHC class I molecule HLA-E, and they can be subdivided into two groups: those that associate with and signal through DAP12 to activate cells, and those that ...
Jin Lei L Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. - - 2014
MicroRNA (miR)-146a is known to be overexpressed in osteoarthritis (OA). However, the role of miR-146a in OA has not yet been fully elucidated. In the present study, we applied mechanical pressure of 10 MPa to human chondrocytes for 60 min in order to investigate the expression of miR-146a and apoptosis following the mechanical ...
Shimo Takenori T Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, Osaka, 565-0871, - - 2014
Antisense-mediated modulation of pre-mRNA splicing is an attractive therapeutic strategy for genetic diseases. Currently, there are few examples of modulation of pre-mRNA splicing using locked nucleic acid (LNA) antisense oligonucleotides, and, in particular, no systematic study has addressed the optimal design of LNA-based splice-switching oligonucleotides (LNA SSOs). Here, we designed ...
Wu Bin B Reproductive Physiology Laboratory, National Research Institute for Family Planning, Beijing 100081, People's Republic of - - 2014
Progesterone (P4) withdrawal triggers endometrial breakdown and shedding during menstruation. Menstruation results from inflammatory responses; however, the role of reactive oxygen species (ROS) in menstruation remains unclear. In this study, we explored the role of ROS in endometrial breakdown and shedding. We found that ROS levels were significantly increased before ...
Murano Kensaku - - 2014
An important characteristic of transcription of a ribosomal RNA gene (rDNA) mediated by DNA-dependent RNA polymerase (Pol) I is its stringent species-specificity. SL1/TIF-IB is a key complex for species-specificity, but its functional complex has not been reconstituted. We established a novel and highly sensitive monitoring system for Pol I transcription ...
Islam Md Soriful MS Department of Experimental and Clinical Medicine, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy; Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi, - - 2014
To determine the effect of tranilast (an antiallergic drug known to suppress fibrosis or to stabilize mast cells) on extracellular matrix production in human leiomyoma and myometrial cells. Laboratory study. University-affiliated laboratory. Seven premenopausal women who were admitted to the hospital for myomectomy or hysterectomy. Cells were treated with tranilast ...
Zhang J George JG Corning Gentest Contract Research Services, Corning Life Sciences, Woburn, MA, USA; - - 2014
Cytochrome P450 (CYP) induction is often considered a liability in drug development. Using calibration curve-based approaches, we assessed the induction parameters R3, relative induction score (RIS), Cmax/EC50 and AUC/F2, derived from concentration-response curves of CYP3A4 mRNA and enzyme activity data in vitro, as predictors of CYP3A4 induction potential in vivo. ...
Chen Ling L Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China; Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, - - 2014
Visceral obesity is an independent risk factor for metabolic syndrome, and abnormal fat accumulation is linked to increases in the number and size of adipocytes. miR-146b was a miRNA highly expressed in mature adipocytes while very low expressed in human mesenchymal stem cells (hMSCs) and human visceral preadipocytes (vHPA). In ...
Chen Kai K Department of Hepatobiliary Surgery Institute, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. - - 2014
The metastasis-related molecules CD133, CD44v6 and human tissue factor (TF) have been shown to be associated with tumor invasion and metastasis. This study aimed to determine whether co-expression of these three molecules was associated with metastasis and overall prognosis in pancreatic carcinoma. We analyzed the expression profiles of these three ...
Feng Lifeng L Laboratory of Cancer Biology; Sir Runrun Shaw Hospital; School of Medicine; Zhejiang University; Hangzhou, Zhejiang - - 2014
Autophagy is a self-proteolytic process that degrades intracellular material to enable cellular survival under unfavorable conditions. However, how autophagy is activated in human carcinogenesis remains largely unknown. Herein we report an epigenetic regulation of autophagy in human cancer cells. YY1 (YY1 transcription factor) is a well-known epigenetic regulator and is ...
Piccolo Luca Lo LL Dipartimento STEBICEF, Istituto Telethon Dulbecco c/o Universita' degli Studi di Palermo, 90128, Palermo, Italy, - - 2014
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a highly conserved family of RNA-binding proteins able to associate with nascent RNAs in order to support their localization, maturation and translation. Research over this last decade has remarked the importance of gene regulatory processes at post-transcriptional level, highlighting the emerging roles of hnRNPs in ...
Lin Li L Department of Nephrology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. - - 2014
MicroRNAs (miRNAs) are a class of small non‑coding single‑stranded RNAs that regulate gene expression at the posttranscriptional level. Since the identification of miRNA, accumulating research has shown their involvement in numerous biological processes, including timing of developmental patterning, embryogenesis, cell differentiation, organogenesis, growth control and pathogenesis of human diseases. It ...
Liu Li-Li LL Sun Yat-sen University Cancer Center; State Key Laboratory of Oncology in South China; Collaborative Innovation Center for Cancer Medicine. Department of Pathology, Sun Yat-sen University Cancer Center. These authors contributed equally to this - - 2014
microRNAs, frequently deregulated in human cancer, have been implicated in the progression of hepatocarcinogenesis. Here, we show that microRNA (miR)-137 is significantly down-regulated in hepatocellular carcinoma (HCC). Its decreased expression is associated with vein invasion, incomplete Involucrum, and distant metastasis. Multivariate analysis suggests that miR-137 is an independent indicator for ...
Antony Lizamma L Chemical Therapeutics Program and Prostate Cancer Program, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland; Department of Urology, The Brady Urological Institute, The Johns Hopkins School of Medicine, Baltimore, - - 2014
Physiologic testosterone continuously stimulates prostate stromal cell secretion of paracrine growth factors (PGFs), which if unopposed would induce hyperplastic overgrowth of normal prostate epithelial cells (PrECs). Lentiviral shRNA stable knock down of c-MYC, β-catenin, or TCF-4 completely inhibits normal (i.e., non-transformed) human PrECs growth. c-MYC enhancer driven reporter expression and ...
Wagner Susan S Laboratory of Regulation of Gene Expression, Institute of Microbiology ASCR, Videnska 1083, Prague, 142 20, the Czech - - 2014
The main role of the translation initiation factor 3 (eIF3) is to orchestrate formation of 43S-48S pre-initiation complexes (PICs). Until now, most of our knowledge on eIF3 functional contribution to regulation of gene expression comes from yeast studies. Hence, here we developed several novel in vivo assays to monitor integrity ...
Dudakovic Amel A Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, - - 2014
Human adipose-derived mesenchymal stromal cells (AMSCs) grown in platelet lysate are promising agents for therapeutic tissue regeneration. Here, we investigated whether manipulation of epigenetic events by the clinically relevant histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) alters differentiation of AMSCs. The multipotency of AMSCs was validated by their ability to ...
Dudognon Benoit B Alternative Gene Expression S.L. (ALGENEX), Centro empresarial, Parque Científico y Tecnológico de la Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Madrid, - - 2014
Growth factors (GFs) are naturally signalling proteins, which bind to specific receptors on the cell surface. Numerous families of GFs have already been identified and remarkable progresses have been made in understanding the pathways that these proteins use to activate/regulate the complex signalling network involved in cell proliferation or wound ...
Rossmann C C Medical University of Graz, Institute of Molecular Biology and Biochemistry, Graz A-8010, - - 2014
Trophoblast invasion into uterine tissues represents a hallmark of first trimester placental development. As expression of serum amyloid A4 (SAA4) occurs in tumorigenic and invasive tissues we here investigated whether SAA4 is present in trophoblast-like human AC1-M59/Jeg-3 cells and trophoblast preparations of human first trimester and term placenta. SAA4 mRNA ...
Donalisio Manuela - - 2014
Strong epidemiological and molecular data associate cervical cancer (CC) to high-risk human papillomavirus (HPV) infections. Carcinogenic mechanism depends mainly on the expression of E6 and E7 oncoproteins encoded by the viral genome. Using a cell-based high-throughput assay, an in-house library of compounds was screened identifying the 1,8-naphthyridone 1 that efficiently ...
Amin Ashok R AR 1 Department of Bio-Medical Engineering, Virginia Tech and Virginia College of Osteopathic Medicine , RheuMatrix, Inc., Blacksburg, - - 2014
We applied global gene expression arrays, quantitative real-time PCR, immunostaining, and functional assays to untangle the role of High Mobility Groups proteins (HMGs) in human Osteoarthritis (OA)-affected cartilage. Bioinformatics analysis showed increased mRNA expression of Damage-Associated Molecular Pattern (DAMPS): HMGA, HMGB, HMGN, SRY, LEF1, HMGB1, MMPs, and HMG/RAGE-interacting molecules (spondins ...
Jeong Hyo-Soon HS Department of Biochemistry, Chung-Ang University College of Medicine, 84 Heukseok-ro, Dongjak-gu, Seoul, 156-756, Republic of - - 2014
Recently, we demonstrated that leucine-rich glioma inactivated 3 (LGI3) is expressed in human skin. However, the effects of LGI3 on melanocytes remain unknown. The present study demonstrated that LGI3 can serve to stimulate melanogenesis without affecting cell viability. To determine the effects of LGI3 on melanin synthesis, normal human melanocytes ...
Jiang Xinye X Department of Child Health Care, Wuxi Maternal and Child Health Hospital, Wuxi, Jiangsu 214002, P.R. - - 2014
White adipose tissue mass is governed by competing processes that control lipid synthesis and storage, as well as the development of new adipocytes, and also trigger metabolic and inflammatory changes. microRNAs (miRNAs) have been suggested to act as negative regulators controlling varied biological processes at the level of post‑transcriptional repression. ...
Hu Chunmei C Department of Oncology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun, 130041, People's Republic of - - 2014
Human phosphatase and tensin homolog (hPTEN) gene was expressed in vascular smooth muscle cells (VSMCs) to study its effect on VSMC proliferation induced in platelet-derived growth factor (PDGF) conditioned medium. After G418 selection, MTT assay was conducted to examine transfected VSMC proliferation induced in human PDGF conditioned medium. We successfully ...
Sachs C C Department of Surgery, Weill Cornell Medical College, New York, NY, - - 2014
The optimal markers for human spermatogonial stem cells (SSCs) are not known. Among the genes recently linked to SSCs in mice and other animals are the basic helix-loop-helix transcription factor ID4 and the orphan G-protein-coupled receptor GPR125. While ID4 and GPR125 are considered putative markers for SSCs, they have not ...
Chien Peter Tzu-Yu PT Graduate Institute of Biomedical Science, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan; Department of Physiology and Pharmacology and Health Ageing Research Center, College of Medicine, Chang Gung University, Tao-Yuan, - - 2014
Different protease-activated receptors (PARs) activated by thrombin have been shown to implicate in various cardiovascular diseases, via up-regulation of inflammatory proteins including cyclooxygeanse-2 (COX-2). However, the mechanisms underlying thrombin-regulated COX-2 expression in human cardiomyocytes remain unclear. Human cardiomyocytes were used in the study. Thrombin-induced COX-2 protein and mRNA expression, and ...
Mattinen Laura - - 2014
Induction of cytochrome P450 (CYP) enzymes is commonly analyzed in cultured human primary hepatocytes (HPHs) by measuring CYP1A2, CYP2B6 and CYP3A4/3A5 activities after exposure to test and reference compounds. Because chemicals can both inhibit and induce CYP enzymes, this traditional approach fails to distinguish such simultaneous effects. Regulatory authorities have ...
Hay Colin W CW School of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, - - 2014
The androgen receptor (AR) is a widely expressed ligand-activated transcription factor which mediates androgen signalling by binding to androgen response elements (AREs) in normal tissue and prostate cancer (PCa). Within tumours, the amount of AR plays a crucial role in determining cell growth, resistance to therapy and progression to fatal ...
Lin Li L Department of Pediatric Pulmonology, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, 109 West Xueyuan Road, Lucheng District, Wenzhou, 325027, Zhejiang, People's Republic of - - 2014
Streptococcus pneumoniae is an important pathogen of pneumonia in human. Human alveolar epithelium acts as an effective barrier and is an active participant in host defense against invasion of bacterial by production of various mediators. Sirtuin 1 (SIRT1), the prototypic class III histone deacetylase, is involved in the molecular control ...
Denizot Jérémy J Clermont Université, M2iSH, UMR 1071 INSERM/Université d'Auvergne, Clermont-Ferrand, France Unité Sous Contrat 2018 Institut National de la Recherche Agronomique, Clermont-Ferrand, - - 2014
Adherent-invasive Escherichia coli (AIEC) are abnormally predominant on Crohn's disease (CD) ileal mucosa. AIEC strains adhere to enterocytes via interaction between type 1 pili and CEACAM6 receptors abnormally expressed on CD ileal mucosa, leading to gut inflammation. We analysed whether epigenetic mechanisms are involved in the upregulation of CEACAM6 expression ...
Johmura Yoshikazu Y Department of Cell Biology, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, - - 2014
Senescence is a state of permanent growth arrest and is a pivotal part of the antitumorigenic barrier in vivo. Although the tumor suppressor activities of p53 and pRb family proteins are essential for the induction of senescence, molecular mechanisms by which these proteins induce senescence are still not clear. Using time-lapse ...
Cernecka Hana H University of - - 2014
β3-Adrenoceptor agonists have recently been introduced for the treatment of the overactive urinary bladder syndrome. Their target, the β3-adrenoceptor, was discovered much later than β1- and β2-adrenoceptors and exhibits unique properties which make extrapolation of findings from the other two subtypes difficult and the β3-adrenoceptor a less understood subtype. This ...
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