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Choi Hyunjung H Bioscience Research Institute, AmorePacific Corporation R&D Center, Yongin, Gyeonggi-do 446-729, Republic of - - 2014
The interactions between human epidermal melanocytes and their cellular microenvironment are important in the regulation of human melanocyte functions or in their malignant transformation into melanoma. Although the basement membrane extracellular matrix (BM-ECM) is one of major melanocyte microenvironments, the effects of BM-ECM on the human melanocyte functions are not ...
Hu XinNan X Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, PR China; School of Public Health, Guangzhou Medical University, Guangzhou 510182, PR - - 2014
2,2',4,4'-Tetra-bromodiphenyl ether (BDE-47), an important congener among polybrominated diphenyl ether (PBDE) compounds, has been predominantly in environmental samples and human tissue. Thyroid disruption is the most sensitive endpoint effect among a number of health effects of exposure to BDE-47 in animals and humans. However, the detailed underlying mechanisms in humans ...
Xiao Yun Y College of Bioinformatics Science and Technology, Harbin Medical University, Harbin 150081, - - 2014
MicroRNAs have been identified as important regulators involved in biological processes and human diseases. We proposed a computational approach to systematic identification of active promoters of miRNAs by active models using epigenetic characteristics at active promoters of protein-coding genes together with a genomic context-based filtering step in nine human cell ...
Peccarelli Megan M Department of Biology, Baylor University, Waco, Texas 76798, - - 2014
The nonsense-mediated mRNA decay (NMD) pathway is a specialized mRNA degradation pathway that degrades select mRNAs. This pathway is conserved in all eukaryotes examined so far and it triggers the degradation of mRNAs that prematurely terminate translation. Originally identified as a pathway that degrades mRNAs with premature termination codons as ...
Rouya Christopher C Department of Biochemistry, McGill University, Montreal, Quebec, H3G 1Y6, Canada Goodman Cancer Research Centre, McGill University, Montreal, Quebec, H3A 1A3, - - 2014
MicroRNAs (miRNAs) play critical roles in a variety of biological processes through widespread effects on protein synthesis. Upon association with the miRNA-induced silencing complex (miRISC), miRNAs repress target mRNA translation and accelerate mRNA decay. Degradation of the mRNA is initiated by shortening of the poly(A) tail by the CCR4-NOT deadenylase ...
Greaves Erin E MRC Centre for Reproductive Health, The University of Edinburgh, Queens Medical Research Institute, 47 Little France Cres, Edinburgh EH16 - - 2014
Context: Ovarian suppression is a common treatment for endometriosis-associated pelvic pain. Its exact mechanism of action is poorly understood although it is assumed to reflect reduced production/ action of estrogens. Objective: To measure expression of mRNAs encoded by nociceptive genes in the peritoneum of women with chronic pelvic pain (CPP) ...
Ha Minju M 1] Center for RNA Research, Institute for Basic Science (IBS), Seoul 151-742, Korea. [2] School of Biological Sciences, Seoul National University, Seoul 151-742, - - 2014
MicroRNAs (miRNAs) are small non-coding RNAs that function as guide molecules in RNA silencing. Targeting most protein-coding transcripts, miRNAs are involved in nearly all developmental and pathological processes in animals. The biogenesis of miRNAs is under tight temporal and spatial control, and their dysregulation is associated with many human diseases, ...
Franasiak Jason M JM Obstetrics, Gynecology and Reproductive Sciences, Robert Wood Johnson Medical School, Rutgers University, Basking Ridge, NJ, USA* Jason M. Franasiak and Katherine A. Burns contributed equally and should both be regarded as joint first - - 2014
C-X-C ligand 13 (CXCL13), a regulator of mucosal immunity, is secreted by human endometrial epithelium and may be involved in embryo implantation. However, cyclic expression of human endometrial CXCL13 in health and disease is not well studied. This study examines cycle stage-specific endometrial CXCL13 expression in normal humans when compared ...
Shi Jia-Xin JX Department of Respiratory Medicine, Lianyungang First People's Hospital, Affiliated Hospital of Xuzhou Medical College, Clinical Medical School of Nanjing Medical University, 182 Northern Tongguan Road, Lianyungang 222002, China. Electronic address: - - 2014
Tristetraprolin (TTP) is an RNA-binding protein which can bind to the AU-rich elements (AREs) at the 3'-untranslated region (3'-UTR) of target mRNA and promote mRNA deadenylation and degradation. We have showed in a previous study that TTP regulates tumor necrosis factor-α (TNF-α)-induced expression of intercellular adhesion molecule-1 (ICAM-1) and interleukin-8 ...
Yaykasli Kursat Oguz KO Department of Medical Biology, Kahramanmaras Sutcu Imam University Medical Faculty, Kahramanmaras, - - 2014
Elucidation of the causes of inflammation has vital importance to development new approach for the treatment of arthritic diseases. The degradation of aggrecan by upregulated disintegrin and metalloproteinase with trombospondin motifs (ADAMTSs) is the key event in the development of both rheumatoid arthritis (RA) and osteoarthritis (OA). Increased levels of ...
Somanathan Suryanarayan S Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of - - 2014
Rationale: Familial hypercholesterolemia (FH) is a genetic disorder that arises due to loss-of-function mutations in the low-density lipoprotein receptor (LDLR) and homozygous FH (hoFH) is a candidate for gene therapy using adeno-associated viral (AAV) vectors. Proprotein convertase subtilisin/kexin type 9 (PCSK9) and inducible degrader of LDLR (IDOL) negatively regulate LDLR ...
Wu Lizheng L State Key Laboratory of Military Stomatology, Department of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, - - 2014
Background/Aims: Tobacco smoking is a major risk factor for the occurrence and progression of periodontitis. We previously demonstrated that nicotine could induce the expression of α7 nicotinic acetylcholine receptors (α7 nAChR) in human and rat periodontal tissues. To further examine the signal pathways mediated by α7 nAChR in periodontal ligament ...
Romani Federica F Istituto di Ginecologia ed Ostetricia, Università Cattolica del Sacro Cuore, Rome, Italy. Electronic address: - - 2014
To evaluate the influence of phthalates on human luteal cell function. Laboratory study. University hospital. Twenty-three normally menstruating patients in the midluteal phase. Human luteal cells isolated from corpora lutea for primary cultures. Progesterone (P4) and prostaglandin release assayed by enzyme immunoassay, vascular endothelial growth factor (VEGF) secretion by enzyme-linked ...
Tarrant-Elorza Margaret M University of Nevada School of Medicine, 1664 North Virginia Street/MS320, Reno, NV 89557, - - 2014
Human cytomegalovirus (HCMV) can establish latent infection in hematopoietic progenitor cells (HPCs) or CD14 (+) monocytes. While circularized viral genomes are observed during latency, how viral genomes persist or which viral factors contribute to genome maintenance and/or replication is unclear. Previously, we identified a HCMV cis-acting viral maintenance element (TR element) ...
Dvorak Zdenek Z Department of Cell Biology and Genetics, Faculty of Science, Palacky University, Slechtitelu 11, 783 71 Olomouc, Czech Republic. - - 2014
Anthocyans are biologically active constituents of various berry fruits and they are also contained in nutritional supplements derived from extracts or dry matter from berry fruits. In this study we evaluated the effects of anthocyans on the expression of selected drug-metabolizing phase II genes in primary cultures of human hepatocytes ...
McSharry Brian P BP Discipline of Infectious Diseases and Immunology, Sydney Medical School, Blackburn Building, University of Sydney, NSW, - - 2014
Regulation of the lectin, Galectin-9 (Gal-9), was investigated for the first time during Human Cytomegalovirus (HCMV) infection. Gal-9 transcription was significantly upregulated in transplant recipients with reactivated HCMV in vivo. In vitro Gal-9 was potently upregulated by HCMV independently of viral gene expression with interferon-β (IFN-β) identified as the mediator ...
Goodarzi Hani H Laboratory of Systems Cancer Biology, Rockefeller University, 1230 York Avenue, New York, New York 10065, - - 2014
Aberrant regulation of RNA stability has an important role in many disease states. Deregulated post-transcriptional modulation, such as that governed by microRNAs targeting linear sequence elements in messenger RNAs, has been implicated in the progression of many cancer types. A defining feature of RNA is its ability to fold into ...
Asakura Mitsutoshi M Graduate School of Pharmaceutical Sciences, Kitasato - - 2014
 Nitrilase, which is found in plants and many types of bacteria, is known as the enzyme that catalyzes hydrolysis of a wide variety of nitrile compounds. While human nitrilase-like protein (NIT), which is a member of the nitrilase superfamily, has two distinct isozymes, NIT1 and NIT2, their function has not ...
Gu Su Yeon - - 2014
The A20 ubiquitin-editing enzyme is a target of nuclear factor kappa B (NF-kappaB) and also plays a key role in regulating the NF-kappaB signaling pathway. NF-kappaB activity is increased during human cytomegalovirus (HCMV) infection and HCMV appears to be adapted to this change. To better understand the regulation of NF-kappaB ...
Vabres Bertrand B Centre Hospitalier, Universitaire de Nantes, Nantes, - - 2014
Human corneal allografting is an established procedure to cure corneal blindness. However, a shortage of human donor corneas as well as compounding economic, cultural, and organizational reasons in many countries limit its widespread use. Artificial corneas as well as porcine corneal xenografts have been considered as possible alternatives. To date, ...
Gan Ana-Maria AM Institute of Cellular Biology and Pathology "Nicolae Simionescu" of the Romanian - - 2014
Fractalkine (Fk) and its receptor CX3CR1 contribute effectively to the atherosclerosis process mediating the recruitment of leukocytes and promoting the interactions between monocytes/ macrophages with smooth muscle cells (SMC). Since Fk expression is significantly increased in SMC during atherogenesis, we aimed to uncover the advanced molecular mechanism of transcriptional regulation ...
Marinova Zoya Z University Clinics for Child and Adolescent Psychiatry, University of Zürich, Zürich, - - 2014
The serotonin 2A receptor (HTR2A) is widely expressed in the brain and involved in the modulation of fear, mood, anxiety and other symptoms. HTR2A and HTR2A gene variations are implicated in depression, schizophrenia, anxiety and obsessive-compulsive disorder. To understand HTR2A signalling changes in psychiatric or neurodegenerative disorders, its normal pattern ...
Biron-Shental Tal T Department of Obstetrics and Gynecology, Meir Medical Center, Kfar Saba, Israel; Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel. Electronic address: - - 2014
Placentas from pregnancies complicated with IUGR (intrauterine growth restriction) express altered telomere homeostasis. In the current study, we examined mechanisms of telomere shortening in these placentas. Placental biopsies from 15 IUGR and 15 healthy control pregnancies were examined. The percentage of trophoblasts with fragmented nuclei: senescence-associated heterochromatin foci (SAHF), was ...
Shao Dongmin D Section of Vascular Biology, National Heart and Lung Institute, Imperial College London, London, - - 2014
Idiopathic pulmonary arterial hypertension (IPAH) is an incurable condition leading to right ventricular failure and death and inflammation is postulated to be associated with vascular remodelling. Interleukin (IL)-33, a member of the "alarmin" family can either act on the membrane ST2 receptor or as a nuclear repressor, to regulate inflammation. ...
Klawitter Marina M Competence Center for Applied Biotechnology and Molecular Medicine, University of Zurich, Zurich, Switzerland, - - 2014
Although inflammatory processes play an essential role in painful intervertebral disc (IVD) degeneration, the underlying regulatory mechanisms are not well understood. This study was designed to investigate the expression, regulation and importance of specific toll-like receptors (TLRs)-which have been shown to play an essential role e.g. in osteoarthritis-during degenerative disc ...
Dong Xiuqing X Center for Biomedicine Research of South China, Fujian Key Laboratory of Developmental and Neuro Biology, College of Life Science, Fujian Normal University, Fuzhou, 350108, Fujian, People's Republic of - - 2014
The developing murine tooth has been used as an excellent model system to study the molecular mechanism of organ development and regeneration. While the expression patterns of numerous regulatory genes have been examined and their roles have begun to be revealed in the developing murine tooth, little is known about ...
Zeng Mei M a Biology Group, North Sichuan Medical - - 2014
Glut9 is highly expressed in the human kidney proximal convoluted tubular and plays a crucial role in the regulation of plasma urate levels. The gene effects were stronger among women. Our results show that 17-β-estradiol (E2) through ER (estrogen receptor) β downregulates Glut9 protein expression on human renal tubular epithelial ...
Gambari Laura L S.C. Laboratorio di Immunoreumatologia e Rigenerazione - - 2014
Hydrogen sulfide (H2S), which recently emerged as a potent regulator of tissues and organs, is broadly produced in mammalian cells but whether it can regulate bone cell function is still elusive. The main objective of this study was to establish the role of H2S in the regulation of human osteoclast ...
Takano Naoharu N Institute for Cell Engineering, Johns Hopkins University School of - - 2014
Cystathionine beta-synthase (CBS) catalyzes metabolic reactions that convert homocysteine to cystathionine. To assess the role of CBS in human glioma, cells were stably transfected with lentiviral vectors encoding short hairpin RNA (shRNA) targeting CBS or a non-targeting control shRNA and subclones were injected into immunodeficient mice. Interestingly, decreased CBS expression ...
Mahajan-Thakur Shailaja S Institut für Pharmakologie, Center of Drug Absorption and Transport, Klinik und Poliklinik für Chirurgie, Abteilung für Allgemeine Chirurgie, Viszeral-, Thorax- und Gefässchirurgie, and Klinik und Poliklinik für Innere Medizin B, Universitätsmedizin Greifswald, Germany; - - 2014
Thrombin is not only a central factor in blood coagulation but also stimulates inflammatory processes, including monocyte responses, via activation of PARs. The signaling lipid S1P is a major determinant of monocyte function. Here, we established an interaction between S1P and human monocyte responses to thrombin. S1P induced PAR-1 and ...
Rebane Ana A Swiss Institute of Allergy and Asthma Research (SIAF), University of Zürich, Davos, Switzerland; Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia. Electronic address: - - 2014
Chronic skin inflammation in atopic dermatitis (AD) is associated with elevated expression of proinflammatory genes and activation of innate immune responses in keratinocytes. microRNAs (miRNAs) are short, single-stranded RNA molecules that silence genes via the degradation of target mRNAs or inhibition of translation. The aim of this study was to ...
Loebel Claudia C AO Research Institute Davos, Davos Platz, Switzerland ; - - 2014
Introduction: Runx2 is one of the most studied transcription factors expressed in Mesenchymal Stem Cells (MSCs) upon their commitment toward an osteogenic differentiation. During endochondral bone formation in-vivo Sox9 directly interacts with Runx2 and represses its activity; however the role of Sox9 in direct osteogenesis in-vitro has been largely overlooked. ...
Sand Kine Marita KM Department of Biosciences and Centre for Immune Regulation, University of Oslo, Oslo, - - 2014
The invariant chain (Ii) is a multifunctional protein, which has an essential role in the assembly and transport of major histocompatibility complex class II (MHC II) molecules. From a single gene, Ii is synthesized as four different isoforms: Iip33, Iip35, Iip41 and Iip43. Iip35 and Iip43 are specific to humans, ...
Vandenberg Cassandra J CJ The Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, - - 2014
Mice susceptible to plasma cell tumors provide a useful model for human multiple myeloma. We have shown previously that mice expressing an Eμ-v-abl oncogene develop solely plasmacytomas. Here we show here that loss of the pro-apoptotic BH3-only protein Bim or, to a lesser extent, over-expression of anti-apoptotic Bcl-2 or Mcl-1, ...
Jensen Louise L LL Department of Biomedicine, University of Aarhus, 8000 Aarhus C., - - 2014
Cubilin plays an essential role in terminal ileum and renal proximal tubules during absorption of vitamin B12 and ligands from the glomerular ultrafiltrate. Cubilin is coexpressed with amnionless, and cubilin and amnionless are mutually dependent on each other for correct processing to the plasma membrane upon synthesis. Patients with defects ...
Huang Chien-Da CD Department of Thoracic Medicine, Chang Gung Memorial Hospital at Linkou, Chang Gung University College of Medicine, Taoyuan, - - 2014
Modification of human airway smooth muscle (ASM) function by proinflammatory cytokines has been regarded as a potential mechanism underlying bronchial hyperresponsiveness in asthma. Human ASM cells express intercellular adhesion molecule (ICAM)-1 in response to cytokines. Synthetic ligands for peroxisome proliferator-activated receptor (PPAR)γ reportedly possess anti-inflammatory and immunomodulatory properties. In this ...
Zhang Guo-You GY Department of Hand and Plastic Surgery, the second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China; Department of Plastic and Reconstructive Surgery, Shanghai ninth People's Hospital of Shanghai Jiaotong University School of Medicine, Shanghai, - - 2014
The proliferation of human skin dermal fibroblasts (HDFs) is a critical step in skin fibrosis, and transforming growth factor-beta1 (TGF-β1) exerts pro-oxidant and fibrogenic effects on HDFs. In addition, the oxidative stress system has been implicated in the pathogenesis of skin disease. However, the role of NADPH oxidase as a ...
Wang Jing J Department of Pharmacology and Toxicology, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY - - 2014
Human oligodendrocyte progenitor cell (OPC) specification and differentiation occurs slowly and limits the potential for cell-based treatment of demyelinating disease. In this study, using FACS-based isolation and microarray analysis, we identified a set of transcription factors expressed by human primary CD140a(+)O4(+) OPCs relative to CD133(+)CD140a(-) neural stem/progenitor cells (NPCs). Among ...
Batista Ribrio I T P RI Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State University of Ceará, Av. Dr. Silas Munguba, 1700, Fortaleza, CE, 60714-903, - - 2014
Human granulocyte-colony stimulating factor (hG-CSF) is a hematopoietic growth factor used in neutropenic patients. It is produced in transgenic bacteria or cultured mammalian cells. As an alternative, we now show that hG-CSF can be expressed in the mammary gland of first-generation (F1) transgenic goats during induced lactation. Despite lower milk ...
Liu Yuxia Y Department of Scientific Research, Peking Union Medical Collage Hospital, Beijing, China; Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University, Beijing, - - 2014
Granulosa cells (GCs) are essential for proper oocyte, follicular development, and steroidogenesis in the ovary. Transforming growth factor β (TGF-β) superfamily members are critical in regulating GCs growth and differentiation. Smad3 is known to serve as a signaling intermediate for the TGF-β; however, the functions of Smad3 in the human ...
Seo Goo-Young GY Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Republic of - - 2014
Retinoic acid (RA) is known to have several functions that lead to a potent mucosal IgA response. Nevertheless, its exact role in human IgA synthesis has yet to be elucidated. Thus, we investigated the role of RA in promoting IgA isotype switching in human B cells. We found that RA ...
Tamura Yu Y Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido - - 2014
Lymphocytic-plasmacytic colitis (LPC) is a common form of inflammatory bowel disease (IBD) affecting the canine large intestine. Cytokines are thought to be involved in the pathogenesis of IBD. However, to date, few studies have investigated cytokine mRNA expression in dogs with LPC. In this study, we investigated mRNA transcription levels ...
Mestdagh Pieter P Center for Medical Genetics, Ghent University, Ghent, - - 2014
MicroRNAs are important negative regulators of protein-coding gene expression and have been studied intensively over the past years. Several measurement platforms have been developed to determine relative miRNA abundance in biological samples using different technologies such as small RNA sequencing, reverse transcription-quantitative PCR (RT-qPCR) and (microarray) hybridization. In this study, ...
TörĊ‘csik D D Department of Dermatology, Faculty of Medicine, University of Debrecen, Debrecen, - - 2014
Leptin, the adipocyte-secreted hormone that regulates weight, is known to link lipid metabolism with inflammation in various cell types, however its role in human sebocytes has not yet been investigated. The purpose of the study was to investigate the effects of leptin in human sebaceous gland biology. Ob-Rb expression was ...
Kaushik Ramakant R Principal Scientist, Embryo Biotechnology Lab, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, 132 001, - - 2014
The aim of the present study was construction of mammary gland specific expression vector for high level of human insulin (hINS) expression in transgenic buffalo for therapeutic use. We have constructed mammary gland specific vector containing human insulin gene and there expression efficiency was checked into in vitro cultured buffalo ...
Esteso Gloria G Department of Immunology and Oncology, Centro Nacional de Biotecnología/Consejo Superior de Investigaciones Científicas, Madrid 28049, - - 2014
Proteolytic shedding of ligands for the NK group 2D (NKG2D) receptor is a strategy used by tumors to modulate immune recognition by NK cells and cytotoxic T cells. A number of metalloproteases, especially those of the a disintegrin and metalloprotease (ADAM) family, can mediate NKG2D ligand cleavage and this process ...
Kemp Jacqueline R JR Department of Molecular Cardiology, Cleveland Clinic, Cleveland, OH, USA; Regulatory Biology Graduate Program, Cleveland State University, Cleveland, OH, - - 2014
Improper regulation of signaling in vascular smooth muscle cells (VSMCs) by angiotensin II (AngII) can lead to hypertension, vascular hypertrophy and atherosclerosis. The extent to which the homeostatic levels of the components of signaling networks are regulated through microRNAs (miRNA) modulated by AngII type 1 receptor (AT1R) in VSMCs is ...
Wojtal Kacper Andrzej KA University Hospital Zurich - - 2014
Solute carrier transporters (SLC) mediate the uptake of biologically active compounds in the intestine. Reduced oxygenation (hypoxia) is an important factor influencing intestinal homeostasis. The aim of this study was to investigate the pathophysiological consequences of hypoxia on the expression and function of SLCs in human intestine. Hypoxia was induced ...
Ni Kai K Department of Urology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Institute of Andrology, No145 Middle Shandong Road, 200001, Shanghai, China; Department of Urology, Pediatric Urology and Andrology, Section Molecular Andrology, Justus Liebig University Giessen, Schubertstraβe. 81, 35392, Giessen, - - 2014
To investigate the expression and the exact role of leptin under hypoxic conditions in human testis. Five testes, obtained from the patients undergoing orchiectomy for prostate cancer, were used to construct an in-vitro hypoxic culture system for human testicular tissue. Immunohistochemistry was performed to analyze leptin-protein expression. The leptin, leptin-R ...
Mikhaylova Marina - - 2014
The survival promoting peptide Y-P30 has a variety of neuritogenic and neuroprotective effects in vitro and in vivo. In previous work we reported the expression of Y-P30/dermcidin in maternal peripheral blood mononuclear cells (PBMCs) and the transport of the protein to the fetal brain. In this study we analyzed hormonal ...
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