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Murakami Ichiro I *Division of Molecular Pathology, Faculty of Medicine ‡Department of Pathobiological Science and Technology, School of Health Science, Faculty of Medicine, Tottori University, Yonago †Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan §Department of Histopathology, The Christie NHS Foundation Trust, Manchester, - - 2014
Merkel cell carcinoma (MCC) is an aggressive neuroendocrine skin cancer, often associated with Merkel cell polyomavirus (MCPyV). Recently, immunoglobulin (Ig) expression was reported in MCC, thereby suggesting that B cells might be their cellular ancestors. We tested 30 MCCs (20 MCPyV-positive and 10 MCPyV-negative) using immunohistochemistry for the expressions of ...
Nakamaru Yuji Y Department of Otolaryngology-Head and Neck Surgery, Hokkaido University Graduate School of Medicine, Sapporo, Japan - - 2014
Many proinflammatory cytokines are regulated by the acetylation and deacetylation of the core histone. Since dysregulation of T helper 2 cytokine production is a key in the pathogenesis of allergic diseases, we examined the role of histone deacetylase (HDAC) on interleukin (IL)-4 gene expression in mast cells. We also examined ...
Rogosch Tobias T Department of Pediatrics, Philipps-University Marburg, D-35033 Marburg, - - 2014
The physiologic function of the "odd" Ab IgG4 remains enigmatic. IgG4 mediates immunotolerance, as, for example, during specific immunotherapy of allergies, but it mediates tissue damage in autoimmune pemphigus vulgaris and "IgG4-related disease." Approximately half of the circulating IgG4 molecules are bispecific owing to their unique ability to exchange half-molecules. ...
Wang Xiaoyu X Division of Gastrointestinal Pathophysiology, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, - - 2014
Over the last few decades, food allergy (FA) has become a common disease in infants in advanced countries. However, anti-allergic medicines available in the market have no effect on FA, and consequently effective drug therapies for FA are not yet available. We have already demonstrated that mucosal mast cells play ...
Benhamron Sandrine S Institute of Drug Research, The Hebrew University of Jerusalem, Jerusalem, - - 2014
Plasma cells (PCs) are responsible for the secretion of antibodies. Development of fully functional PCs relies on the activation of the IRE1/XBP-1 arm of the unfolded protein response (UPR). XBP-1-deficient PCs poorly secrete antibodies and exhibit distensions of the endoplasmic reticulum (ER). The kinase mTOR promotes anabolic activities and is ...
Van Vaerenbergh Matthias M Laboratory of Molecular Entomology and Bee Pathology, Ghent University, Krijgslaan 281 S2, B-9000 Ghent, - - 2015
Api m 10 has recently been established as novel major allergen that is recognized by more than 60% of honeybee venom (HBV) allergic patients. Previous studies suggest Api m 10 protein heterogeneity which may have implications for diagnosis and immunotherapy of HBV allergy. In the present study, RT-PCR revealed the ...
Kravchenko Irina V IV Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskiy Prospect 33, 119071 Moscow, - - 2014
Expression of insulin-like growth factor 1 (IGF-1) mRNAs splice forms was recently shown to be stimulated by myofibrillar proteins released from the damaged muscle. In this study, we report that individual subfragments of titin and myomesin composed of Fn type III and Ig-like domains can activate expression of two IGF-1 ...
Yang Nan N Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, New - - 2014
Currently, there is no satisfactory treatment for IgE-mediated food allergy. Food Allergy Herbal Formula 2 (FAHF-2) and butanol-purified FAHF-2 (B-FAHF-2) have been shown to protect against peanut-induced anaphylaxis and inhibit IgE synthesis in a murine model. To determine which herbs and compounds in FAHF-2 and B-FAHF-2 suppress IgE production. The ...
Spanic Tanja T Center for Animal Genomics, Veterinary Faculty, University of Ljubljana , Ljubljana , Slovenia - - 2014
Abstract The presence of steroidogenic enzymes in the brain suggests de novo synthesis of steroid hormones in the brain. The current study was designed to determine the developmental profiles of cytochrome p450 aromatase (cyp19), 17β-hydroxysteroid dehydrogenase (17β-HSD), 5α-reductase type I and 3α-hydroxysteroid dehydrogenase (3α-HSD) mRNA expression levels in the fetal ...
Zheng Ye Y Laboratory of Translational Epigenetics in - - 2014
Endocrine regulation of uterine biology is critical for embryo receptivity and human reproduction. Uterine endometrium depends on extrinsic sex steroid input and hence likely has mechanisms that enable adaptation to hormonal variation. Emerging evidence suggests that sex steroid bioavailability in the endometrium is determined by adjusting their metabolic rate and ...
Gao Li-Na LN Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China; Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, - - 2014
Genipin is obtained from the fruit of Gardenia jasminoides Ellis and acts as herbal medicine or functional food in East Asia. In addition to produce natural colorant, it possesses widely antiinflammatory, antithrombotic, antidepressive and anticarcinogenic activities. However, little research focuses on the potential of genipin for drug-drug interactions. In this ...
Simone S S Nephrology, Dialysis and Transplantation Unit, Dept. of Emergency and Organ Transplantation, University of Bari "Aldo - - 2014
NADPH oxidase plays a central role in mediating oxidative stress during heart, liver and lung ischemia/reperfusion injury, but limited information is available about NADPH oxidase in renal ischemia/reperfusion injury. Our aim was to investigate the activation of NADPH oxidase in a swine model of renal ischemia/reperfusion damage. We induced renal ...
Han X F XF Isotope Research Lab, Sichuan Agricultural University, Ya'an, People's Republic of - - 2014
To investigate the effects of antitestosterone immunization, initiated during early puberty, on hypothalamic-pituitary-testicular feedback in rabbits, 16 early pubertal male rabbits were randomly allocated into 2 groups (n = 8), control or immunized against testosterone-3(O-carboxymethyl)oxime-BSA in Freund adjuvant at 4 mo of age (with a booster immunization 4 wk later). Blood samples ...
Aung Htin Lin HL Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand. Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, Private Bag 92019, Auckland 1042, New - - 2014
Mycobacteria are obligate aerobes and respire using two terminal respiratory oxidases, an aa3-type cytochrome c oxidase and a cytochrome bd-type menaquinol oxidase. Cytochrome bd is encoded by cydAB from the cydABDC gene cluster that is conserved throughout the mycobacterial genus. Here, we report that cydAB and cydDC in Mycobacterium smegmatis ...
Dermauw V V Laboratory of Animal Nutrition, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, B-9820 Merelbeke, Belgium - - 2014
Copper deficiency is a commonly diagnosed problem in cattle around the globe. In Jimma, Ethiopia, 8 zebu (Bos indicus) and 8 zebu × Holstein Friesian cross (Bos taurus × Bos indicus) heifers were used in an 11 wk study, to investigate breed type differences and effects of Cu deficiency on ...
Jun Young Joon YJ Department of Otorhinolaryngology-Head & Neck Surgery, College of Medicine, Soonchunhayng University, Kumi Hospital, Kyungsangbuk-Do, - - 2014
It has been suggested that glucocorticoids might act in target tissues to increase their own intracellular availability in response to inflammatory stimuli. These mechanisms depend on the local metabolism of glucocorticoids catalyzed by 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) and 11β-hydroxysteroid dehydrogenase 2 (11β-HSD2). This study is to investigate the effect of ...
Tripathi Anubhuti A Division of Pharmacokinetics and Metabolism, CSIR-Central Drug Research Institute, Lucknow-226 031, - - 2014
Hormone replacement therapy and selective estrogen receptor modulator are the most common therapy for women going through menopause. These therapies though popular fail to relieve withdrawal symptoms such as hot flashes, fatigue, leg cramps and nausea. This scenario necessitates to herbal preparations as alternative which may lead to simultaneous intake ...
Shimamoto Yoshinori Y Department of Veterinary Teaching Hospital, School of Veterinary Medicine, Kitasato - - 2014
To examine the effect of Babesia infection on the level of the drug-metabolizing enzyme hepatic cytochrome P450 (CYP) 2D, we intraperitoneally inoculated Babesia microti into male ICR mice. CYP2D protein and CYP2D9 mRNA were significantly decreased at 12 days after infection with B. microti. The activity of bunitrolol 4-hydroxylase, which ...
Huang Guo-Yong GY State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address: - - 2014
The aim of this study was to evaluate the effects of three metals (Zn, Cd and Pb) on hepatic metallothionein (MT), cytochrome P450 1A (CYP1A) and vitellogenin (Vtg) mRNA expression in the liver of adult female mosquitofish (Gambusia affinis) after 1, 3 or 8d. Both concentration-response and time-course effects of ...
Bremer Katharina K Queen's University, - - 2014
Steady-state mRNA levels are determined by synthesis and degradation; however, changes in mRNA levels are usually attributed to transcription. For cytochrome c oxidase (COX), cold acclimation typically leads to an increase in COX activity while transcript levels for the nuclear-encoded subunits change non-stoichiometrically. Whether those patterns are caused by differences ...
Zheng Binghui B State Key Laboratory of Environmental Criteria and Risk Assessment & State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environment Sciences, Beijing 100012. Electronic address: - - 2014
Metabolizing enzymes play important roles in the detoxification of various pollutants in aquatic organisms, thereby they can also be used to provide early-warning signals of environmental risks. Real-time quantitative reverse-transcription polymerase chain reaction assays were developed to quantify cytochrome P450 1A (CYP1A), superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), ...
Chang Ziwei Z Department of Chemistry and Chemistry Institute of Functional Materials, Pusan National University, Busan 609-735, - - 2014
Cytochrome P4501A (CYP1A) in fish has attracted an increasing interest because of its important roles in the metabolic activation of certain xenobiotics such as aromatic hydrocarbons. CYPs are reported to be expressed in yeast, insect cells and Escherichia coli, but are critical for high-level expression. Besides, this study found that ...
Curtis Kevin M KM Geriatric Research, Education, and Clinical Center and Research Service, Bruce W. Carter Veterans Affairs Medical Center, Miami, FL 33125, USA; Departments - - 2014
Although 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3) is considered the most biologically active vitamin D3 metabolite, the vitamin D3 pro-hormone, 25-hydroxyvitamin D3 (25(OH)D3), is metabolized into other forms, including 24R,25-dihydroxyvitamin D3 (24R,25(OH)2D3). Herein we show that 24R,25(OH)2D3 is fundamental for osteoblastic differentiation of human mesenchymal stem cells (hMSC). Our approach involved analyses of ...
Melnik Bodo C BC Department of Dermatology, Environmental Medicine and Health Theory, University of Osnabrück, - - 2014
In this issue Hellmann-Regen et al. suggested that antiacne effects of erythromycin and tetracyclines may be related to their inhibitory effect of cytochrome P450-mediated degradation of all-trans-retinoic acid (ATRA). We have recently proposed that all antiacne agents function by attenuation of increased mTORC1-signaling. This commentary links the P450-system to mTORC1-regulation in ...
Takahata Toru T Department of Psychology, Vanderbilt University, Nashville, TN - - 2014
Ocular dominance columns (ODCs) have been well studied in the striate cortex (V1) of macaques, as well defined arrays of columnar structure that receive inputs from one eye or the other, whereas ODC expression seems more obscure in some New World primate species. ODCs have been identified by means of ...
Duda Malgorzata M Department of Endocrinology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387, Cracow, Poland, - - 2014
We used our model system for agonism and antagonism of the androgen receptor (AR), in which the porcine ovarian follicles were exposed on the excessive concentration of an AR agonist- testosterone (T) or an AR antagonist- 2-hydroxyflutamide (2-Hf) to: (1) analyze the spatiotemporal expression of ovarian 3β-hydroxysteroid dehydrogenase (3β-HSD), cytochrome ...
Hoshino Motohiro M Department of Clinical Pharmacokinetics, Hoshi - - 2014
Recently, it was reported that the anticoagulant effect of warfarin was reduced when patients receiving warfarin also took menthol. The purpose of this study is to reveal the mechanism of this reduced anticoagulant effect of warfarin from the pharmacokinetic point of view. Warfarin was orally administered to mice 24 h ...
Lamba V V Departments of Experimental and Clinical Pharmacology, University of Minnesota, Minneapolis, MN. Electronic address: - - 2014
microRNA (miRNA) mediated regulation of gene expression has emerged as a significant mechanism contributing to variation in gene expression. In this study, we evaluated the potential role of miRNAs in regulating expression of hepatic cytochrome P450s and their transcriptional regulatory genes. We screened the Targetscan database for high scoring miRNA ...
Liu Su S State Key Laboratory of Rice Biology; Key Laboratory of Agricultural Entomology, Ministry of Agriculture; and Institute of Insect Sciences, Zhejiang University, Hangzhou, China; Department of Entomology, College of Plant Protection, Anhui Agricultural University, Hefei, - - 2014
NADPH-cytochrome P450 reductase (CPR) is essential for numerous biological reactions catalyzed by microsomal cytochrome P450 monooxygenases (P450s). Knockdown of CPR in several insects leads to developmental defects and increased susceptibility to insecticides. However, information about the role of CPR in the brown planthopper, Nilaparvata lugens, is still unavailable. A full-length ...
Moriya N - - 2014
Certain physiological states and diseases can alter the expression and activity of cytochrome P450 s (CYPs), which have the potential to cause unexpected adverse effects. We previously demonstrated that lipopolysaccharide (LPS)-induced inflammation attenuates the induction of CYPs by xenobiotics in mouse liver. In this study, to investigate whether anaphylaxis-induced inflammation ...
Kasimanickam V V Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, - - 2014
Pharmacological approaches to control spermatogenesis are required to resolve overpopulation in dogs. The objective of the study was to investigate the regulation of meiosis-associated and male germ cell-related genes, stimulated by retinoic acid gene 8 (STRA8), synaptonemal complex protein 3 (SYCP3), dosage suppressor of mck1 (DMC1), doublesex and mab-3 related ...
Klichko Vladimir - - 2014
The phenotypic effects of under- and over-expression of cytochrome c oxidase (CcO) regulatory subunits IV and Vb were examined in Drosophila melanogaster in order to further test the hypothesis that suppression of the activities of mitochondrial electron transport chain (ETC) oxidoreductases retards the aging process and extends life span. Under-expression ...
Cui Tie-Zhong TZ University of Utah, United - - 2014
Yeast deficient in the Rieske iron-sulfur subunit (Rip1) of ubiquinol-cytochrome c reductase (bc1) accumulate a late core assembly intermediate, which weakly associates with cytochrome oxidase (CcO) in a respiratory supercomplex. Expression of the N-terminal half of Rip1 which lacks the C-terminal FeS-containing globular domain (designated N-Rip1) results in a marked ...
Frerigmann Henning H Department of Molecular Plant Physiology, BioCenter, Botanical Institute and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne Cologne, - - 2014
To balance the flux of sulfur (S) into glucosinolates (GSL) and primary metabolites plants exploit various regulatory mechanisms particularly important upon S deficiency (-S). The role of MYB34, MYB51 and MYB122 controlling the production of indolic glucosinolates (IGs) and MYB28, MYB29, and MYB76 regulating the biosynthesis of aliphatic glucosinolates (AGs) ...
Oliveira-Pelegrin Gabriela R GR Departamento de Morfologia, Fisiologia e Patologia Básica, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: - - 2013
We investigated whether inflammatory mediators during cecal ligation and puncture (CLP)-induced sepsis may diminish copeptin expression in magnocellular neurons, thus affecting arginine-vasopressin (AVP) synthesis. The transcript abundance of IL-1β, IL-1R1, iNOS and HIF-1α was continuously elevated. IL-1β, iNOS and cytochrome c protein levels progressively increased until 24h. Immunostaining for these ...
Smutny Tomas - - 2013
Pregnane X receptor (PXR) is a member of the nuclear receptor (NR) superfamily of ligand-activated transcription factors and is activated by a huge variety of endobiotics and xenobiotics, including many clinical drugs. PXR plays key roles not only as a xenosensor in the regulation of both major phase I and ...
Ishii Makoto - - 2013
We had previously revealed that drug metabolism, as well as the expression level of hepatic cytochrome P450 3A (CYP3A), a drug-metabolizing enzyme, increase 12 weeks after gastrectomy in mice. In this study, we elucidated the mechanism of the increased CYP3A expression. The levels of lithocholic acid (LCA)-producing bacteria (Bacteroides fragilis) ...
Van Peer Els - - 2013
Despite the increasing use of the minipig as a non-rodent species in general and juvenile toxicity studies, knowledge on their biotransformation processes and their ontogeny is scarce. Such data are prerequisite for the correct interpretation of non-clinical studies in this species. Therefore, the aim of our investigation was to immunohistochemically ...
Feger Martina - - 2013
Background/Aims: Carbon monoxide (CO) interferes with cytochrome-dependent cellular functions and acts as gaseous transmitter. CO is released from CO-releasing molecules (CORM) including tricarbonyl-dichlororuthenium (II) dimer (CORM-2), molecules considered for the treatment of several disorders including vascular dysfunction, inflammation, tissue ischemia and organ rejection. Cytochrome P450-sensitive function include formation of 1,25-dihydroxyvitamin ...
Zhu Yi - - 2013
Tissue-specific deletion of the gene for NADPH-cytochrome P450 reductase (CPR), the essential electron donor to all microsomal cytochrome P450 (P450/CYP) enzymes, in either liver or intestine leads to up-regulation of many P450 genes in the tissue with the Cpr deletion. Here, by studying the liver-specific Cpr-null (LCN) mouse, we examined ...
Mimche Sylvie M - - 2013
Inflammation and infection down-regulate the activity and expression of cytochrome P450s (P450s) and other drug metabolizing enzymes (DMEs) involved in hepatic drug clearance. Schistosoma mansoni infection was reported to cause a down-regulation of hepatic P450-dependent activities in mouse liver, but little is known about the specific enzymes affected or whether ...
Anwar-Mohamed Anwar - - 2013
Activation of the aryl hydrocarbon receptor (AhR) ultimately leads to the induction of the carcinogen activating enzyme cytochrome P450 1A1 (CYP1A1). While activation of the nuclear factor-erythroid 2 p45-related factor 2 (Nrf2) in addition to the AhR pathway induces the expression of the NADP(H): quinone oxidoreductase (NQO1). Therefore, the aim ...
Franczak A - - 2013
Estradiol-17β (E2) is a potent regulator of early pregnancy and the estrous cycle in pigs. Production of E2 occurs in the porcine myometrium, but the factors involved in its regulation are unknown. In this in vitro study, it was investigated whether interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α affect the ...
Pereira Renato Tavares Dos Santos - - 2013
The aim of the present study was to investigate the effects of different periods of ovariectomy and 17β-estradiol (E2) replacement on the expression of Cytochrome C, apoptosis inducing factor (AIF) and Endonuclease-G (Endo-G) in mitochondrial and cytosolic fractions obtained from hippocampus of the adult female rats. In addition, the expression ...
Kobayashi Satoshi - - 2013
In the n-alkane assimilating yeast Yarrowia lipolytica, the expression of ALK1, encoding a cytochrome P450 that catalyzes terminal mono-oxygenation of n-alkanes, is induced by n-alkanes. The transcription of ALK1 is regulated by a heterocomplex that comprises the basic helix-loop-helix transcription activators, Yas1p and Yas2p, and binds to alkane-responsive element 1 ...
Li Xiaoyu - - 2013
Cytochrome P450s (CYPs) play a key role in the metabolism of a wide range of environmental xenobiotics and endogenous compounds. The expression and activity levels of CYPs can be elevated by a process of induction involving the activation of nuclear receptors. The effects of the ionic liquid 1-octyl-3-methylimidazolium chloride ([C8mim][Cl]) ...
Johar Kaid K Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin, - - 2013
Neurons are highly dependent on oxidative metabolism for their energy supply, and cytochrome c oxidase (COX) is a key energy-generating enzyme in the mitochondria. A unique feature of COX is that it is one of only four proteins in mammalian cells that are bigenomically regulated. Of its thirteen subunits, three ...
Zelasko Susan - - 2013
Recombinant expression of membrane-bound cytochrome P450s in bacterial expression systems provide a well-established system capable of producing large yields of catalytically active protein. As the biochemical knowledge regarding cytochrome P450s increases, so does the efficiency of protein expression through various modifications that do not disrupt the functional properties of the ...
Tralau Tewes - - 2013
Introduction: Over the past years, there has been increasing evidence that, at least in vertebrates, cytochrome P450-dependent monooxygenases (CYPs) are governed by a most complex regulation. The respective mechanisms comprise structural features such as domain movements, allostery, enzyme-oligomerization as well as numerous transcription factors, non-coding RNAs and extensive regulatory crosstalk. ...
Ueno Motoi - - 2013
Bacterial cytochrome P450 enzymes catalyze the oxidative biotransformation of various types of compounds. Although the functional expression of Actinomycetes P450 in a closely related heterologous host can serve as a useful biocatalyst in whole-cell biotransformation assays, the co-expression of an electron transfer partner is required. To overcome this limitation, P450Rhf ...
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