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Yang Hao H Department of Spine Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, - - 2014
Abstract Syndecan-4 is emerging as an important player in cell interaction with the extracellular environment and has been shown to be involved in the progression of intervertebral disc degeneration. However, the mechanism of syndecan-4 regulation by TNF-α and the role of TGF-β1 in regulating syndecan-4 expression remain poorly understood in ...
Sun Zhongyi Z 1Shanghai First People's Hospital affiliated Shanghai Jiao Tong University, School of Medicine, Department of Orthopedics 2Huai'an First People's Hospital, Nanjing Medical University, Department of - - 2014
Study Design. IL-1β (Interleukin-1β) can activate human nucleus pulposus(NP) cells with or without nuclear factor kappa B(NF-kB) inhibition. We undertook a descriptive and mechanistic investigation of catabolic effects of NF-kB signaling pathway in intervertebral disc(IVD) degenerative changes.Objective. To clarify the mediatory role of NF-kB signaling pathway in human intervertebral disc ...
Nishida-Aoki Nao N Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, - - 2014
In Saccharomyces cerevisiae, we have demonstrated that organic solvent stress activated the pleiotropic drug resistance (PDR) pathway, which involves the transcription factors Pdr1p and Pdr3p. Pdr1p and Pdr3p are functionally homologous in multidrug resistance, although Pdr3p has been reported to have some distinct functions. Here, we analyzed the functions of ...
Porcelli Letizia L National Cancer Institute Giovanni Paolo II: Viale Orazio Flacco, 65, 70124 Bari, Italy. - - 2014
While multidrug resistance (MDR) in cancer is well established, little is known about the cellular pathways regulating the expression and trafficking of the MDR efflux transporter like BCRP (ABCG2). Here we evaluated the role of signalling downstream of EGFR on BCRP expression and sub-cellular localization using lung cancer cells harboring ...
Watanabe Toshiaki T Yale University - - 2014
The eukaryotic genome has vast intergenic regions containing transposons, pseudogenes, and other repetitive sequences. They produce numerous long non-coding RNAs (lncRNAs) and PIWI-interacting RNAs (piRNAs), yet the functions of the vast intergenic regions remain largely unknown. Mammalian piRNAs are abundantly expressed in late spermatocytes and round spermatids, coinciding with the ...
Sun Kuo-Ting KT Graduate Institute of Clinical Medical Science, China Medical University, No. 91 Hsueh-Shih Rd, Taichung, Taiwan; Department of Pediatric Dentistry, China Medical University Hospital, No. 2 Yu-Der Rd., Taichung, Taiwan; School of Dentistry, China Medical University, No. 91 Hsueh-Shih Rd, Taichung, - - 2014
Autophagy and autophagy-related proteins (ATGs) play decisive roles in osteoclast differentiation. Emerging lines of evidence show that deregulation of miRNA in autophagic responses. However, the role of hypoxia and involvement of miRNA in osteoclast differentiation is unclear. In the present study, we demonstrate that hypoxia caused induction of autophagy and ...
Kumar Roshan M RM 1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA [2] Howard Hughes Medical Institute, Department of Biomedical Engineering, Center of Synthetic Biology, Boston University, Boston, Massachusetts 02215, - - 2014
Pluripotent stem cells (PSCs) are capable of dynamic interconversion between distinct substates; however, the regulatory circuits specifying these states and enabling transitions between them are not well understood. Here we set out to characterize transcriptional heterogeneity in mouse PSCs by single-cell expression profiling under different chemical and genetic perturbations. Signalling ...
Kim Seung-Kyoon SK Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of - - 2014
LIN28-mediated processing of the microRNA (miRNA) let-7 has emerged as a multilevel program that controls self-renewal in embryonic stem cells. LIN28A is believed to act primarily in the cytoplasm together with TUT4/7 to prevent final maturation of let-7 by Dicer, whereas LIN28B has been suggested to preferentially act on nuclear ...
Wang Liang L Department of Orthopedic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 1 Shuaifuyuan Hutong, Beijing 100730, - - 2014
Long non-coding RNAs (lncRNAs) have been established to participate in various biological processes that are crucial for development and differentiation. However, the roles of lncRNAs in the mechanisms of human bone marrow mesenchymal stem cells (MSCs) differentiation are not completely understood. The purpose of the study was to investigate the ...
Tejedor J Ramón JR Centre de Regulació Genòmica, Dr. Aiguader 88, 08003 Barcelona, Spain; Universitat Pompeu Fabra, Dr. Aiguader 88, 08003 Barcelona, - - 2014
Alternative splicing of Fas/CD95 exon 6 generates either a membrane-bound receptor that promotes, or a soluble isoform that inhibits, apoptosis. Using an automatized genome-wide siRNA screening for alternative splicing regulators of endogenous transcripts in mammalian cells, we identified 200 genes whose knockdown modulates the ratio between Fas/CD95 isoforms. These include ...
Pirrello Julien - - 2014
BackgroundLatex harvesting in Heveabrasiliensis amounts to strong abiotic stress that can cause a halt in production in the most susceptible clones. Although the role of jasmonic acid has been suggested in laticifer differentiation, its role in latex production and in the response to harvesting stress has received very little attention. ...
Manning Sean S Centre for Obesity Research, Rayne Institute, Department of Medicine, University College London, London WC1E 6JJ, UK; School of Medicine, University College Cork, Cork, - - 2014
Central MC4R pathways are well established as integrators of peripheral signals in the control of energy balance. In this issue, Panaro et al. (2014) identify that MC4Rs expressed on intestinal enteroendocrine L cells regulate PYY and GLP-1 secretion, two gut hormones implicated in the regulation of energy and glucose homeostasis.
Jiang Z Z Center for Regenerative Biology, Department of Animal Science, University of Connecticut, Storrs, Connecticut, - - 2014
The SWI/SNF [SWItch/Sucrose NonFermentable; also known as BAF (Brg/Brahma-associated factors)] ATP-dependent chromatin remodelling complexes are epigenetic modifiers that change the structure of chromatin, and thereby modulate gene transcription. The BAF chromatin remodeling complexes undergo progressive changes in subunit composition during cellular differentiation. In embryonic stem cells (ESC), BAF complex, esBAF, ...
Zhang Shuxin S Center for Plant Science Innovation & School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of - - 2014
The evolutionary conserved WD-40 protein PRL1 plays important roles in immunity and development. Here we show that PRL1 is required for the accumulation of microRNAs (miRNAs) and small interfering RNAs (siRNAs). PRL1 positively influences the processing of miRNA primary transcripts (pri-miRNAs) and double-stranded RNAs (dsRNAs). Furthermore, PRL1 interacts with the ...
Pellizzaro Anthoni A Université d'Angers, MR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, 2 Boulevard Lavoisier, F-49045, Angers, France; INRA, UMR1345 Institut de Recherche en Horticulture et Semences, 42 rue Georges Morel, F-49071, Beaucouzé, - - 2014
Nitrate transporters received little attention in legumes probably because these species are able to adapt to N starvation by developing biological N2 fixation. Still it is important to study nitrate transport systems in legumes because nitrate intervenes as a signal in regulation of nodulation probably through nitrate transporters. The aim ...
Yuan Guohua G The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China Department of Cell and Molecular Biology, Tulane University, New Orleans, LA 70118, - - 2014
BMP and Wnt signaling pathways play a crucial role in organogenesis, including tooth development. Despite extensive studies, the exact functions, as well as if and how these two pathways act coordinately in regulating early tooth development, remain elusive. In this study, we dissected regulatory functions of BMP and Wnt pathways ...
Carvalho Lara L Instituto de Medicina Molecular, Edifício Egas Moniz, 1649-028 Lisbon, Portugal; and Centro de Estudos de Doenças Crónicas, NOVA Medical School/Faculdade de Ciências Médicas, Universidade Nova de Lisboa, 1169-056 Lisbon, - - 2014
The Toll/NF-κB pathway, first identified in studies of dorsal-ventral polarity in the early Drosophila embryo, is well known for its role in the innate immune response. Here, we reveal that the Toll/NF-κB pathway is essential for wound closure in late Drosophila embryos. Toll mutants and Dif dorsal (NF-κB) double mutants ...
Liao Anyan A Department of Radiation Oncology, Peking University 3rd Hospital, No. 49 Hua Yuan Bei Lu, Beijing, 100191, China, - - 2014
Stromal cell-derived factor 1 (SDF-1)/CXCR4 ligand-receptor axis is widely recommended as an attractive target for cancer therapy. Meanwhile, epithelial-mesenchymal transition (EMT) process is linked to disease pathophysiology. As one of inhibitors of apoptosis proteins, survivin is implicated in the onset and development of cancer. In the present study, we tried ...
Ahmed Mohammed I MI Centre for Skin Sciences, School of Life Sciences, University of Bradford, Bradford BD7 1DP, England, - - 2014
Skin development is governed by complex programs of gene activation and silencing, including microRNA-dependent modulation of gene expression. Here, we show that miR-214 regulates skin morphogenesis and hair follicle (HF) cycling by targeting β-catenin, a key component of the Wnt signaling pathway. miR-214 exhibits differential expression patterns in the skin ...
Li S S 1] Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, People's Republic of China [2] Department of Developmental Biology, UT Southwestern Medical Center at Dallas, 6000 Harry Hines Boulevard, Dallas, TX 75390-9133, - - 2014
c-Myc is a key transcriptional factor that has a prominent role in cell growth, differentiation and tumor development. Its protein levels are tightly controlled by ubiquitin-proteasome pathway and frequently deregulated in various cancers. Here, we report that the 11S proteasomal activator REGγ is a novel regulator of c-Myc abundance in ...
Haslam I S IS The Centre for Dermatology Research, Institute of Inflammation and Repair, University of Manchester, Manchester, M13 9PT, United - - 2014
Adenosine triphosphate binding cassette (ABC) transporters are involved in the active transport of an extremely diverse range of substrates across biological membranes. These transporters are commonly implicated in the development of multidrug resistance and are also involved in numerous physiological and homeostatic processes, including lipid transport, cell migration and differentiation. ...
Das Dipon D KIIT School of Biotechnology, KIIT University, Campus-11, Patia, Bhubaneswar, Orissa 751024, - - 2014
Colorectal cancer (CRC) patients with APC mutations do not benefit from 5-FU therapy. It was reported that APC physically interacts with POLβ and FEN1, thus blocking LP-BER via APC's DNA repair inhibitory (DRI) domain in vitro. The aim of this study was to elucidate how APC status affects BER and ...
Bowman Christopher John CJ Department of Pathology, New York University Cancer Institute, New York University School of Medicine, New York, New York 10016, - - 2014
Autophagy is the primary catabolic process triggered in response to starvation. Although autophagic regulation within the cytosolic compartment is well established, it is becoming clear that nuclear events also regulate the induction or repression of autophagy. Nevertheless, a thorough understanding of the mechanisms by which sequence-specific transcription factors modulate expression ...
Stoll Katrin K Institut Molekulare Botanik, Universität Ulm, D-89069, Ulm, - - 2014
Plant mitochondrial transcripts undergo maturation processes at both termini. Although frequently observed the post-transcriptional formation of mature 5' ends is still poorly understood. We now analyzed the processing of transcripts derived from the mitochondrial ccmC gene, coding for a component of the cytochrome c maturation system. In Arabidopsis thaliana (Arabidopsis) ...
Yamagishi Ryota R Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, - - 2014
The poly(A) tail of mRNAs plays pivotal roles in the posttranscriptional control of gene expression at both translation and mRNA stability. Recent findings demonstrate that the poly(A) tail is globally stabilized by some stresses. However, the mechanism underlying this phenomenon has not been elucidated. Here, we show that arsenite-induced oxidative ...
Tan Gao G Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, - - 2014
Human enteric α-defensins (HD5 and HD6), major antimicrobial peptides produced by Paneth cells in the intestine, play important roles in intestinal innate immunity. Since their expression is decreased in Crohn's disease (CD), with decreased expression being more pronounced in the presence of NOD2 mutations, it would be extremely interesting to ...
Ibrahim B A BA Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of Louisiana at Monroe, Monroe, LA 71201, United - - 2014
Dorsal vagal complex (DVC) AMPK regulation of food intake in the estradiol-treated ovariectomized (OVX) female rat is energy state-dependent. Here, RT-PCR array technology was used to identify estradiol-sensitive AMPK-regulated DVC signal transduction pathways that exhibit differential reactivity to sensor activation during energy balance versus imbalance. The AMP mimetic AICAR correspondingly ...
Simms Carrie L CL Department of Biology, Washington University in St. Louis, St. Louis, MO 63130, - - 2014
Chemical damage to RNA affects its functional properties and thus may pose a significant hurdle to the translational apparatus; however, the effects of damaged mRNA on the speed and accuracy of the decoding process and their interplay with quality-control processes are not known. Here, we systematically explore the effects of oxidative ...
Ryu Seung-Wook SW Department of Bio and Brain Engineering, KI for the - - 2014
The shape and activity of mitochondria are tightly regulated by fusion and fission processes that are essential for maintaining normal cellular function. However, little is known about the involvement of mitochondrial dynamics in the development of the immune system. In this study, we demonstrate that mitochondrial dynamics play a role ...
Parr Callum C Division of Brain Sciences, Imperial College London, London, United Kingdom; - - 2014
Alterations in the Wnt signaling pathway have been implicated in Alzheimer's disease; however, its role in the processing of the amyloid precursor protein remains unknown. In this study, activation of the Wnt pathway by overexpression of the agonist Wnt3a or β-catenin or by inhibition of glycogen kinase synthase-3 in N2a ...
Der Vartanian Audrey A INRA, UMR 1061 Unité de Génétique Moléculaire Animale, Université de Limoges, Faculté des Sciences et Techniques, 123 avenue A. Thomas, 87060 Limoges Cedex, - - 2014
The Notch signaling pathway plays a crucial role in skeletal muscle regeneration in mammals by controlling the transition of satellite cells from quiescence to an activated state, their proliferation and their commitment towards myotubes or self-renewal. O-fucosylation on Notch receptor EGF-like repeats is catalyzed by the protein O-fucosyltranferase 1 (Pofut1) ...
Valenzuela-Miranda Diego D Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, P. O. Box 160-C, Concepción, - - 2014
Despite the increasing evidence of the importance of microRNAs (miRNAs) in the regulation of multiple biological processes, the molecular bases supporting this regulation are still barely understood in crustaceans. Therefore, the molecular characterization and transcriptome modulation of the miRNA biogenesis pathway were evaluated in the salmon louse Caligus rogercresseyi, an ...
Dang Ningning N Department of Dermatology, Jinan Central Hospital Affiliated to Shandong University, No. 105 Jiefang Road, Jinan, 250013, Shandong Province, China, - - 2014
We aimed to gain further insight into the role of the MAPK signaling pathway in terminal differentiation of normal human epidermal keratinocytes (NHEKs) with filaggrin knockdown. Filaggrin expression was knocked down by shRNA technology and the MAPK pathways were inhibited by three different inhibitors in NHEKs. The associated mRNAs and ...
Busek Petr P Laboratory of Cancer Cell Biology, Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University in Prague, U Nemocnice 5, 128 53, Prague 2, Czech Republic, - - 2014
Fibroblast activation protein (FAP, seprase, EC 3.4.21.B28) and dipeptidyl peptidase-IV (DPP-IV, CD26, EC 3.4.14.5) are homologous serine proteases implicated in the modulation of the bioavailability and thus the function of a number of biologically active peptides. In spite of their generally nonoverlapping expression patterns, DPP-IV and FAP are co-expressed and ...
Siwaszek Aleksandra - - 2014
The process of mRNA decay and surveillance is considered to be one of the main posttranscriptional gene expression regulation platforms in eukaryotes. The degradation of stable, protein-coding transcripts is normally initiated by removal of the poly(A) tail followed by 5'-cap hydrolysis and degradation of the remaining mRNA body by Xrn1. ...
Ibrahem Salih S Academic Unit of Molecular Pathology, Nottingham University, Nottingham, - - 2014
Wnt signalling and the signal transducer and activator of transcription 3 (STAT3) are oncogenic signalling pathways which are deregulated in colorectal cancer (CRC). Here we investigated the interaction of these two pathways. Firstly, we investigated biochemical interaction by inhibiting STAT3 and β-catenin (through gene knock-down and dominant-negative TCF4 expression) in ...
Zana-Taieb E E Université Paris Descartes, Paris, France; Fondation PremUp, 53 avenue de l'Observatoire, 75014, Paris, France; Institut National de la Santé Et de la Recherche Médicale (INSERM) U1141, Paris, France; Assistance Publique - Hôpitaux de Paris, Service de Médecine et Réanimation néonatales de Port-Royal, Groupe hospitalier Cochin, Broca, Hôtel-Dieu. 53 avenue de l'Observatoire, 75014, Paris, - - 2014
Intrauterine growth restriction (IUGR) dramatically increases the risk of bronchopulmonary dysplasia in preterm babies, a disease characterised by arrested alveolarisation and abnormal microvascular angiogenesis. We have previously described a rodent low protein diet (LPD) model of IUGR inducing impaired alveolarisation, but failed to demonstrate any modification of the classical factors ...
Blackney Michael J - - 2014
In this study we cloned and sequenced the mRNAs of the extracellular Cu Zn superoxide dismutase (Sod3) gene in Drosophila and identified two mRNA products formed by alternative splicing. These products code for a long and short protein derived from the four transcripts found in global expression studies (Flybase numbers ...
Thaker Youg Raj YR Cell Signalling Section, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom. Electronic address: - - 2014
The transcription factor NF-κB is needed for the induction of inflammatory responses in T-cells. Whether its activation by the antigen-receptor and CD28 is mediated by the same or different intracellular signaling pathways has been unclear. Here, using T-cells from various knock-out (Cd28(-/-), adap(-/-)) and knock-in (i.e. Cd28 Y-170F) mice in ...
Dean Zachary S ZS Biomedical Engineering Interdisciplinary Program, The University of Arizona, Tucson, AZ 85721-0119, - - 2014
MicroRNAs (miRNAs) are small, noncoding RNAs variably involved in a wide variety of developmental and regenerative programs. Techniques for monitoring the spatiotemporal expression of miRNA in living cells are essential to elucidate the roles of miRNA during these complex regulatory processes. The small size, low abundance, sequence similarity, and degradation ...
Dubey Richa R Functional Genomics Unit, Institute of Genomics and Integrative Biology (CSIR), Mall Road, Delhi-110007, - - 2014
MiRNAs and transcription factors have emerged as important regulators for gene expression and are known to regulate various biological processes including cell proliferation, differentiation and apoptosis. Previously, using genome wide expression profiling studies we have shown an inverse relationship of STAT6 and cholesterol biosynthesis and also identified FOXJ2 binding sites ...
Rodriguez Stephanie N SN Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, United - - 2015
Self-peptide MHCII ligands are critical for selection of CD4+ T cells in the thymus, and maintenance in the periphery. To date, no investigation as to the exact thymic and peripheral expression of a naturally occurring positive selecting self-peptide MHCII (self-pMHCII) complex has taken place. We have generated a sensitive T ...
Valenti Fabio F Sbarro Institute for Cancer Research and Molecular Medicine, College of Science and Technology, Temple University, Philadelphia, Pennsylvania, 19122, USA; Department of Biology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania, 19122, - - 2014
β-catenin is a central effector of the Wnt pathway and one of the players in Ca(+) -dependent cell-cell adhesion. While many wnts are present and expressed in vertebrates, only one β-catenin exists in the majority of the organisms. One intriguing exception is zebrafish that carries two genes for β-catenin. The ...
Petrova Olga E OE Department of Biological Sciences, Binghamton University, 85 Murray Hill Road, Binghamton, NY - - 2014
Biofilm dispersion is a highly regulated process allowing biofilm bacteria to respond to changing environmental conditions and to disseminate to new locations. Dispersion of biofilms formed by the opportunistic pathogen Pseudomonas aeruginosa is known to require a number of c-di-GMP-degrading phosphodiesterases (PDEs) and the chemosensory protein BdlA, with BdlA playing ...
Lu Han H a Department of Anesthesiology, Ruijin Hospital, Shanghai Jiao-Tong University School of Medicine (SJTU-SM) , Shanghai , People's Republic of - - 2014
Abstract Activity-dependent stimuli induced a calcineurin-mediated dephosphorylation of the transcriptional factor MEF2A at serine408 and promoted a switch from SUMOylation to acetylation at lysine403 which led to MEF2A transcriptional activation. We previously identified SENP2 is the de-SUMOylation enzyme for MEF2A and promotes MEF2A-dependent transcription. We report here a requirement for ...
Tocco Alice A Université de Bordeaux, CIRID UMR 5164, F-33000, Bordeaux, - - 2014
Adenosine is an endogenous molecule that regulates many physiological processes via the activation of four specific G-protein-coupled ADORA receptors. Extracellular adenosine may originate either from the hydrolysis of released ATP by the ectonucleotidases or from cellular exit via the equilibrative nucleoside transporters (SLC29A). Adenosine extracellular concentration is also regulated by ...
Tomar Dhanendra D Department of Cell Biology, School of Biological Sciences and Biotechnology, Indian Institute of Advanced Research, Gandhinagar, - - 2014
The nuclear factor κB (NF-κB) transcription factor family plays a key role in regulation of the inflammatory pathway in response to different physiological stimuli starting from development to ageing. The dysregulation of NF-κB has been associated with many pathological conditions like inflammatory diseases, neurodegeneration, metabolic diseases and various kinds of ...
Rozenberg Julian M JM From the Department of Pathology, University of North Carolina, Chapel - - 2014
The goal of the present study was to identify novel mechanisms that regulate smooth muscle cell (SMC) differentiation marker gene expression. We demonstrate that the CArG-containing regions of many SMC-specific promoters are imbedded within CpG islands. A previously identified GC repressor element in the SM myosin heavy chain (MHC) promoter ...
Di Giammartino Dafne Campigli DC Department of Biological Sciences, Columbia University, New York, New York 10027, - - 2014
Polyadenylation of mRNA precursors is mediated by a large multisubunit protein complex. Here we show that RBBP6 (retinoblastoma-binding protein 6), identified initially as an Rb- and p53-binding protein, is a component of this complex and functions in 3' processing in vitro and in vivo. RBBP6 associates with other core factors, ...
Benesch Franziska F Veterinär-Physiologisches Institut - - 2014
In the intact rumen epithelium, isoforms 1 and 4 of the monocarboxylate transporter (MCT1 and MCT4) are thought to play key roles in mediating transcellular and intracellular permeation of short-chain fatty acids and their metabolites and in maintaining intracellular pH. We examined whether both MCT1 and MCT4 are expressed at ...
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