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Gao Chaobing C Department of Otorhinolaryngology, Head and Neck Surgery, First Affiliated Hospital of AnHui Medical University , Hefei , - - 2014
Abstract Conclusions: Increased expression of Dicer may be a prognostic biomarker for patients with laryngeal squamous cell carcinoma (LSCC). Recent studies have shown that many microRNAs (miRNAs) play an important role in the development and progression of human cancers. Dicer, one of the most important enzymes of the miRNA machinery, ...
Pan Yuan Y Department of Biochemistry, Carver College of Medicine, University of Iowa, 51 Newton Road, Biochemistry, 4-712 BSB, Iowa City, IA, 52242, - - 2014
Hyperpolarization-activated cyclic nucleotide-gated 1 (HCN1) channels carry Ih, which contributes to neuronal excitability and signal transmission in the nervous system. Controlling the trafficking of HCN1 is an important aspect of its regulation, yet the details of this process are poorly understood. Here, we investigated how the C-terminus of HCN1 regulates ...
Margarit Ezequiel E Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) - Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Ocampo y Esmeralda, (S2000FHQ) Rosario, - - 2014
Cellular nucleic acid binding protein (CNBP) is a small and highly conserved protein with nucleic acid chaperone activity that binds single-stranded nucleic acids. Data collected so far suggests that CNBP is required for proper craniofacial development. Despite the advances achieved in the last decade, the identity of the molecular targets ...
Yu Jianshe J School of Mathematics and Information Science, Guangzhou University, Guangzhou, 510006, China; Key Laboratory of Mathematics and Interdisciplinary Sciences of Guangdong Higher Education Institutes, Guangzhou University, Guangzhou, 510006, China. Electronic address: - - 2014
Gene transcription is a stochastic process, and is often activated by multiple signal transduction pathways. In this work, we study gene transcription activated randomly by two cross-talking pathways, with the messenger RNA (mRNA) molecules being produced in a simple birth and death process. We derive the analytical formulas for the ...
Perez-Martin Marta M CSIC-Universidad de - - 2014
The accumulation of unfolded/misfolded proteins in the endoplasmic reticulum (ER) results in the activation of stress responses such as the unfolded protein response or the catabolic process of autophagy to ultimately recover cellular homeostasis. ER stress also promotes the production of reactive oxygen species (ROS), which play an important role ...
Dai Wei-Ping WP Department of Gastroenterology, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, - - 2014
Epithelial-mesenchymal transition (EMT) process and extracellular signal-regulated kinase 1 (ERK1) signaling pathway play pivotal roles in hepatic stellate cell (HSC) activation, which is associated with the altered expression patterns of microRNAs (miRNAs). miR-155 is considered a typical multifunctional miRNA to regulate many biological processes. However, little attention has been given ...
Peng Xiao Hong - - 2014
The molecular mechanisms underlying dysregulation of microRNAs have been documented in nasopharyngeal carcinoma (NPC). Our previous study demonstrated that plasma miR-124 was down-regulated in NPC using microarray analysis and quantitative PCR validation. Though growing studies showed that down-regulated miR-124 was closely related to tumourigenesis in various types of cancers, the ...
Xu Donghua D Department of Rheumatology, the First Affiliated Hospital, Nanjing Medical University, 210029, Nanjing, - - 2014
Protein tyrosine phosphatase receptor type O (PTPRO) has been identified as a tumor suppressor in a number of cancers including hepatocellular carcinoma (HCC). Toll-like receptor 4 (TLR4) plays diverse roles in HCC tumorigenesis and progression. The association between PTPRO and TLR4 signaling in HCC remains largely unknown. We aimed to ...
Poi Ming J MJ Department of Pharmacy, The Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, Columbus, OH, U.S.A Division of Pharmacy Practice and Administration, College of Pharmacy, The Ohio State University, Columbus, OH, - - 2014
Background/Aim: While aberrant expression of cyclin-dependent kinase-4 (CDK4) has been found in squamous cell carcinoma of the head and neck (SCCHN), the associations between CDK4 and its regulators, namely, cyclin D1, cyclin E, gankyrin, SEI1, and BMI1 in gene expression remain to be explored. Herein we investigated the mRNA profiles ...
Giesen Eva E Yale Cancer Center, Yale University School of Medicine, New Haven, CT, U.S. These authors contributed equally to this - - 2014
Background: Novel immune therapies targeting tumor specific antigens are being developed. Our purpose was to determine expression of the cancer testes antigen NY-ESO-1 in renal cell carcinoma (RCC), as NY-ESO-1 targeting approaches, particularly adoptive cell therapy, have not been evaluated in this disease. Methods: We employed tissue microarrays containing >300 ...
Miyoshi Kina K Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan; Department of Pediatric Surgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, - - 2014
CXC chemokine receptor 4 (CXCR4) expression is reportedly correlated with both vascular endothelial growth factor (VEGF) expression and poor prognosis in a variety of cancers. Its relation to CXC chemokine receptor 7 (CXCR7) is also noted in several malignancies, including rhabdomyosarcoma (RMS) cell lines. However, the correlations between these chemokine ...
Liu Zhiyong Z Department of Pathology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, China; The Key Laboratory of Tianjin Cancer Prevention and Treatment, Tianjin, 300060, China; National Clinical Research Center for Cancer, Tianjin, 300060, - - 2014
Dickkopf-1 (Dkk1), an antagonist of wnt/β-catenin signaling pathway, has been reported to play a role in cancer progression. However, little is known about the role of Dkk1 during colorectal adenoma-carcinoma sequence. This study aimed to elucidate the role of Dkk1 in tumorigenesis and angiogenesis in colorectal cancer. we examined Dkk1 ...
Wang Lei - - 2014
The introduction of cisplatin has improved the long-term survival rate in osteosarcoma patients. However, some patients are intrinsically resistant to cisplatin. This study reported that the activation of Notch1 is positively correlated with cisplatin sensitivity, evidenced by both clinical and in vitro data. In this study, a total 8 osteosarcoma ...
Feng Ai-Ping AP Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China, - - 2014
The deubiquitinating enzyme ubiquitin specific peptidase 15 (USP15) is regarded as a regulator of TGFβ signaling pathway. This process depends on Smad7, the inhibitory factor of the TGFβ signal, and type I TGFβ receptor (TβR-I), one of the receptors of TGFβ. The expression level of USP15 seems to play vital ...
Vieten D D Department of Paediatric Surgery, Bristol Royal Hospital for Children, Bristol, UK Mucin Research Group, University of Bristol, Bristol, - - 2014
The pivotal role of epidermal growth factor receptor (EGFR) in transducing signalling by multiple ligands involved in proliferation, differentiation and migration is evidenced by the fact that EGFR-null mice invariably die in utero or early post-natal life. Inactivation of EGFR in knockout mice results in a haemorrhagic enterocolitis similar to ...
Vieten D D Department of Paediatric Surgery, Bristol Royal Hospital for Children, Bristol, UK Mucin Research Group, University of Bristol, Bristol, - - 2014
The aetiology of necrotizing enterocolitis (NEC) is multifactorial and its pathogenesis poorly understood. The trefoil factor peptides (TFF1-3) contribute to protective mechanisms operating in the gastro-intestinal (GI) mucosa and play a fundamental role in epithelial protection, restitution and repair. The role of TFF1-3 in neonatal mucosal protection has not been ...
Shin Su-Jin SJ Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, - - 2014
Multiple myeloma (MM) is a heterogeneous and ultimately fatal disease. Risk stratification using prognostic biomarkers is crucial to individualize treatments. We sought to investigate the role of CD99, a transmembrane protein highly expressed in many hematopoietic cells including subpopulations of normal and neoplastic plasma cells, for MM risk stratification. CD99 ...
Ahn Ei Yong - - 2014
Telomere dysfunction is important in carcinogenesis, and recently, stathmin and elongation factor 1alpha (EF1alpha) were reported to be up-regulated in telomere dysfunctional mice. In the present study, the expression levels of stathmin and EF1alpha in relation to telomere length, telomere dysfunction-induced foci (TIF), gamma-H2AX, and p21WAF1/CIP1 expression were assessed in ...
Moura Caio M CM Laboratory of Medical Investigation (LIM 55), University of Sao Paulo Medical School, Sao Paulo - - - 2014
CD44 is a transmembrane glycoprotein and is regarded as a potential marker in various tumors. The aim of our study was to analyze the expression of the standard form of CD44 (CD44s) and its isoforms in localized prostate cancer (PCa), and to correlate these data with the classical prognostic factors ...
Davidson Ben B Department of Pathology, Oslo University Hospital, Norwegian Radium Hospital, N-0424, Oslo, Norway, - - 2014
The objective of this study was to investigate the expression and clinical role of the spindle checkpoint kinase budding uninhibited by benzimidazole 1 (Bub1) in primary and metastatic advanced-stage ovarian serous carcinoma. BUB1 mRNA expression was analyzed in 178 tumors (88 effusions, 38 primary carcinomas, and 52 solid metastases) from ...
Wang D - - 2014
Purpose : The objective of this study was to develop a sensitive, specific and rapid approach to diagnose hand foot and mouth disease (HFMD) for an early treatment by using loop-mediated isothermal amplification (LAMP) technique. Materials and Methods : A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) for detecting EV71 virus was ...
Mazibrada Jasenka J Department of Oncology, University of Turin at San Luigi Hospital, Turin, - - 2014
The aim of this study was to analyze protein and gene expression of HER2 in 224 head and neck precancerous and malignant lesions by immunohistochemistry and FISH analysis. In parallel, expression of pStat3, Sox2, IFI16 and p16, Ki67 was evaluated. Immunohistochemical analysis was assessed on formalin-fixed paraffin-embedded (FFPE) tissue specimens. ...
Wang Liang L Department of Basic Medicine, School of Medicine, Shenzhen University, Shenzhen, Guangdong 518060, P.R. - - 2014
The aim of the current study was to investigate the expression of cyclin‑dependent kinase 3 (CDK3) in human nasopharyngeal carcinoma (NPC) and to evaluate its association with the clinicopathological characteristics of patients with NPC. CDK3 expression was examined in three NPC cell lines and one nasopharyngeal epithelial cell line by western blot analysis ...
Carrarelli Patrizia P Department of Molecular and Developmental Medicine, University of Siena, Siena, - - 2014
To evaluate antimüllerian hormone (AMH) and AMH receptor II (AMHRII) mRNA and protein expression in endometrium and in ovarian or deep lesions of women with endometriosis. Prospective study. University hospitals in Italy and Brazil. Patients with endometriosis (n = 55) and healthy women (n = 45). Specimens of endometrium obtained by hysteroscopy from patients ...
Janusicova Veronika V Departments of 1Obstetrics and Gynecology and 2Molecular Biology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovak - - 2014
The objective of current study was to determine the p16 mRNA level in cervical cells by relative quantification (RQ) and to test viral E6 expression in human papillomavirus (HPV) -16 or -18-positive specimens by widely used methods. We targeted the pivotal mRNA level associated with severe dysplasia or worse. Cervical ...
Tang Shao Hui SH Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, - - 2014
Hepatitis B virus (HBV) X protein (HBx) contributes to hepatocarcinogenesis. The overexpression of transcripts from P3 and P4 promoters of the insulin-like growth factor 2 (IGF2) gene is observed in hepatocellular carcinoma (HCC). Here we aimed to explore the involvement of HBx in P3-driven mRNA overexpression and underlying epigenetic mechanism. ...
Li Tianhong T *University of California Davis Comprehensive Cancer Center, †Department of Internal Medicine, Division of Hematology & Oncology, University of California, Davis, School of Medicine, Sacramento, California; ‡Department of General-, Visceral- and Tumor Surgery, University of Cologne, Cologne, Germany; §Response Genetics, Inc., Los Angeles, California; ‖Department of Public Health Sciences, University of California, Davis, Davis, California; ¶Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California; and #formerly Response Genetics, Inc., Los Angeles, - - 2014
The objective of this study was to identify and characterize echinoderm microtubule-associated protein-like 4 anaplastic lymphoma kinase fusion (EML4-ALK+) cancers by variant-specific, quantitative reverse transcription polymerase chain reaction (RT-PCR) assays in a large cohort of North American non-small-cell lung cancer (NSCLC) patients. We developed a panel of single and multiplex ...
Nobusawa Aiko A Department of Diagnostic Pathology, Gunma University Graduate School of Medicine, Maebashi, Japan; Department of Stomatology and Maxillofacial Surgery, Gunma University Graduate School of Medicine, Maebashi, - - 2014
Diagnosis of the exact grade of oral epithelial dysplasia is difficult, and interobserver variations in grading are common. The aim of this study was to investigate the expression patterns of cytokeratins (CKs) in dysplastic oral epithelia, to identify useful double immunostaining diagnostic markers. Immunoexpression of CK13, CK14, CK17, and Ki-67 ...
Morgan Sherif S - - 2014
Serum protein acidic and rich in cysteine (SPARC) is a matricellular secreted glycoprotein that performs several cellular functions and has been implicated in tumorigenesis in a variety of tumor types. The chemotherapeutic agent nanoparticle albumin-encapsulated (NAB)-paclitaxel has been postulated to exploit SPARC expression to target neoplastic cells. SPARC's role, and ...
Zheng Yan Y State Key Laboratory of Oncology in South China and Department of Experimental Research, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China; Department of Gastric and Pancreatic Surgery, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of - - 2014
The aim of this study was to investigate the expression and prognostic significance of Uroplakin1A (UPK1A) in gastric adenocarcinoma patients. Functional studies were also analyzed in vitro. Real-time quantitative PCR (RT-qPCR), western blotting, and immunohistochemical (IHC) staining methods were used to analyze the expression of UPK1A in primary gastric adenocarcinoma ...
Behan M - - 2014
Interest has increased in CDX2 gene expression in oesophageal non-goblet-cell columnar metaplasia as recent investigations indicate such metaplasia possesses neoplastic potential. This study aims to assess expression of the transcription factor CDX2 specifically in non-goblet-cell cardia and fundic oesophageal metaplastic tissue, and to compare the location of CDX2 expression in ...
Tang Cui C Department of Biochemistry and Molecular Biology, Chongqing Medical University Chongqing, - - 2014
Clinical and epidemiological studies have shown that estrogen may be involved in the development and progression of papillary thyroid carcinoma (PTC). G protein-coupled estrogen receptor 1 (GPER1) is a novel seven-transmembrane estrogen receptor that functions alongside traditional nuclear estrogen receptors (ERs) to regulate the cellular responses to estrogen. The purpose ...
Gorka Oliver O Institut für Klinische Chemie und Pathobiochemie, Klinikum rechts der Isar, Technische Universität München, 81675, Munich, - - 2014
Upon activation of CD4+ T helper cells the transcription factor complex NF-κB becomes activated and its subunits are able to translocate to the nucleus. There, the dimerized proteins regulate the transcription of for example cytokine and pro-survival genes. This process is of central importance for a proper function of T ...
Micera Alessandra A IRCCS-G.B. Bietti Foundation, Rome, Italy, - - 2013
To assess the expression of toll-like receptors (TLRs) in human amniotic membrane (AM) specimens and compare this expression with those of AMs undergoing the standard preservation procedure (handling) for ocular surgery. Human fresh (n = 10; five spontaneous and five cesarean) or handled (n = 5) AMs were analyzed for TLR gene and protein ...
Dong Yi Wei YW Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Key Lab of Glycoconjugate Research, Ministry of Public Health, Shanghai, - - 2013
The biological relevance and regulation mechanism of aberrant miR-223 expression in human hepatocellular carcinoma (HCC) remain unknown. Our aim was to investigate miR-223 regulation in HCC. miR-223 and integrin αV dysregulation were verified in 57 HCC specimens. Immunohistochemical analysis of integrin αV and sulfatide levels was performed on another cohort ...
Powell Steven S Division of Hematology, Oncology and Transplantation, Department of Medicine, University of Minnesota, Minneapolis, MN 55418, USA ; Hematology/Oncology Section, Primary Care Service Line, Minneapolis VA Healthcare System, Minneapolis, MN 55417, - - 2014
Class III β-tubulin (TUBB3) is emerging as a biomarker in a number of cancers. TUBB3 has been shown to be a prognostic indicator of more aggressive disease and a predictor of resistance to taxanes and vinca alkaloids. To date, there is little data on TUBB3 expression in small cell lung ...
Nishikawa Masatomo - - 2014
The objective of this study was to analyze the expression levels of multiple components in the mammalian target of rapamycin (mTOR) signaling pathway in radical nephrectomy specimens from patients with metastatic renal-cell carcinoma (RCC) treated with mTOR inhibitors in order to identify factors predicting susceptibility to these agents. This study ...
Saratsis Amanda M - - 2013
Diffuse intrinsic pontine glioma (DIPG) is a highly morbid form of pediatric brainstem glioma. Here, we present the first comprehensive protein, mRNA, and methylation profiles of fresh-frozen DIPG specimens (n = 14), normal brain tissue (n = 10), and other pediatric brain tumors (n = 17). Protein profiling identified 2,305 unique proteins indicating distinct DIPG protein ...
Quagliata Luca - - 2013
Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide. The lack of effective therapeutic options for advanced stage HCCs combined with an increasing incidence rate calls for the identification of early stage HCC molecular markers. SH2 Domain Containing 4A (SH2D4A) gene maps to human chromosome 8p21.3 and ...
Poi Ming J - - 2013
The presence of RD(INK4/ARF) (RD) enhancer in the INK4-ARF locus provides a novel mechanism to simultaneously increase the transcription of p15(INK4b) (p15), p14ARF (p14), and p16(INK4a) (p16). While such upregulation can be repressed through interactions between RD and oncoproteins CDC6 and BMI1, little is known about the involvement of RD ...
Li Shi-Sheng - - 2013
Recent research has demonstrated that tropomyosin‑related kinase B (TrkB) plays an important role in neuronal survival, differentiation and migration; yet, its specific role in human nasopharyngeal carcinoma (NPC) is unclear. To elucidate its role in NPC, we examined TrkB expression in NPC tissues and cell lines, and investigated the correlation between ...
Zhang Qun - - 2013
The purpose of the study is to analyze the relationship between tumor thymidylate synthase (TS) mRNA expression levels and raltitrexed/pemetrexed/5-FU sensitivity. We collected freshly removed colorectal tumor specimens from 50 patients. Chemosensitivities to anticancer drugs were evaluated by histoculture drug response assay. We adopted quantitative reverse transcription polymerase chain reaction ...
Wang Ni - - 2013
TGF-β1, SNAI1 and MMP-9 are implicated in tumor invasion and metastasis. The purpose of this study was to examine TGF-β1, SNAI1 and MMP-9 expression in papillary thyroid carcinoma (PTC), and to assess association of TGF-β1, SNAI1 and MMP-9 expression with several clinicopathological indicators of PTC. TGF-β1, SNAI1 and MMP-9 protein ...
Zhang Zhi-Qiang ZQ Zhi-Qiang Zhang, Yu Xia, Department of Gastroenterology, the First Affiliated Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uyghur Autonomous Region, - - 2013
To study the differential expression of Annexin A1 (ANXA1) protein in human gastric adenocarcinoma. This study was also designed to analyze the relationship between ANXA1 expression and the clinicopathological parameters of gastric carcinoma. Purified gastric adenocarcinoma cells (GAC) and normal gastric epithelial cells (NGEC) were obtained from 15 patients with ...
Akanuma N - - 2013
Background:FSCN1 and matrix metalloproteinase 14 (MMP14) are both invadopodia-related proteins. We herein elucidate the tumourigenicity of these proteins and identify novel therapeutic agents in esophageal squamous cell carcinoma (ESCC).Methods:FSCN1 and MMP14 were evaluated by immunohistochemistry and quantitative PCR, and microRNA (miR)-133a was also evaluated by PCR in surgical ESCC specimens. ...
Hsieh Shu-Chen - - 2013
This study aimed at investigating the expression and functional significance of seven in absentia homologues 2 (SIAH2), an E3 ubiquitin ligase, in oral squamous cell carcinoma (OSCC). Comparative genomic hybridization and real-time PCR were performed to examine the amplification of SIAH2 gene in clinical specimens of OSCC tissues. Expression of ...
Wang Jing J Institute of Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Nanjing Medical University, Nanjing, Jiangsu - - 2013
Marsupialisation or decompression is frequently performed as a conservative therapy for keratocystic odontogenic tumours (KCOTs). Positive cyclooxygenase-2 (COX-2) expression in the epithelium of KCOTs was recently reported and may be associated with neoplastic invasion and progression. The aim of the present study was to investigate the change in COX-2 expression ...
Xie Fang F Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, - - 2013
Sonic hedgehog (Shh) signaling has been extensively studied and is implicated in various inflammatory diseases and malignant tumors. We summarized the clinicopathological features and performed immunohistochemistry assays to examine expression of Shh signaling proteins in 10 normal mucosa, 32 gallbladder carcinoma (GBC), and 95 chronic cholecystitis (CC) specimens. The CC ...
Dayi Nimet - - 2013
Hyperplastic polyps (HP) and sessile serrated adenomas (SSA) share morphological similarities. In this immunohistochemical study we chose a panel of potential relevant and promising biomarkers including alpha-methylacyl-coenzyme A racemase (AMACR;p504s) , which is involved in the degradation of branched chained fatty acids derivates, and analysed a cohort of HPs and ...
Velpula Kiran Kumar - - 2013
Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor in adults. Overexpression of the EGF receptor (EGFR) is recognized as a widespread oncogenic signature in GBM, but the complexity of its contributions are not fully understood, nor the most effective ways to leverage anti-EGFR therapy in this setting. Hypoxia ...
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