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Yeap Wan-Chin WC Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Sime Darby Technology Centre Sdn. Bhd., 1st Floor, Block B, UPM-MTDC Technology Centre III, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, - - 2014
Plant cells contain a diverse repertoire of RNA-binding proteins (RBPs) that coordinate a network of post-transcriptional regulation. RBPs govern diverse developmental processes by modulating the gene expression of specific transcripts. Recent gene annotation and RNA sequencing clearly showed that heterogeneous nuclear ribonucleoprotein (hnRNP)-like proteins which form a family of RBPs, ...
Liao Jian-You JY State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China; Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Research Center of Medicine, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, People's Republic of - - 2014
Small RNAs (sRNAs), including microRNAs and endogenous siRNAs (endo-siRNAs), regulate most important biologic processes in eukaryotes, such as cell division and differentiation. Although sRNAs have been extensively studied in various eukaryotes, the role of sRNAs in the early emergence of eukaryotes is unclear. To address these questions, we deep sequenced ...
Aphasizheva Inna I Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, - - 2014
Enzymes embedded into the RNA editing core complex (RECC) catalyze the U-insertion/deletion editing cascade to generate open reading frames in trypanosomal mitochondrial mRNAs. The sequential reactions of mRNA cleavage, U-addition or removal, and ligation are directed by guide RNAs (gRNAs). We combined proteomic, genetic and functional studies with sequencing of ...
Yang Tsuey-Ching TC Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, 112, Taiwan. Electronic address: - - 2014
Xanthomonas campestris pv. campestris expresses a chromosomally encoded class A β-lactamase BlaXc. Basal expression and induction of blaXc require the transcriptional factor AmpRXc and the peptidoglycan-monomers permease AmpGXc. NagZ is a β-GlcNAcase which cleaves GlcNAc-anhMurNAc peptides (peptidoglycan-monomers) to generate anhMurNAc-peptides. In many bacteria, anhMurNAc-peptides act as activation ligands for AmpR. ...
Pézeron Guillaume - - 2014
There is growing evidence that Notch pathway activation can result in consequences on cell morphogenesis and behaviour, both during embryonic development and cancer progression. In general, Notch is proposed to co-ordinate these processes by regulating expression of key transcription factors. However, many Notch-regulated genes identified in genome-wide studies are involved ...
Larabee Shannon M SM Georgetown University Medical Center, Biochemistry and Molecular & Cellular Biology, Washington, District of Columbia, United States ; - - 2014
Body axes and germ layers evolve at gastrulation, and in mammals are driven by many genes; however, what orchestrates the genetic pathways during gastrulation remains elusive. Previously, we presented evidence that microRNA-17 family members, miR-17-5p, miR-20a, miR-93, and miR-106a were differentially expressed in mouse embryos and functioned to control differentiation ...
Joseph Noah N The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 5290002, - - 2014
The Nck adapter protein is involved in key cellular functions, such as actin polymerization and reorganization, serving as a molecular bridge between the surface complex essential for foreign antigen recognition, the T-cell antigen receptor (TCR), and the actin machinery. However, the mechanisms regulating Nck expression and functions are unknown. In ...
Ji Qinghong - - 2014
CUL4B, a scaffold protein that assembles CRL4B ubiquitin ligase complex, participates in the regulation of a broad spectrum of biological processes. We here demonstrated a critical role of CUL4B in driving cell cycle progression. We showed that loss of CUL4B resulted in a significantly reduced cell proliferation and G1 cell ...
Chassot Anne-Amandine AA A Chassot, iBV, Université de Nice-Sophia Antipolis, UMR-INSERM1091, Nice, France - - 2014
Sex differentiation is a unique developmental process. Starting from a bipotential gonad, it gives rise to the ovary and the testis, two highly specialized organs which differ morphologically and physiologically despite sharing common reproductive and endocrine functions. This highlights the specific plasticity of the gonadal precursors and the existence of ...
Yang Xiaoling X Department of Pathophysiology, Basic Medical School, Ningxia Medical University, Key Laboratory of Cardio-Cerebro-Vascular Diseases, Ningxia Medical University, Yinchuan 750004, - - 2014
Endoplasmic reticulum (ER) stress is emerging as an important modulator of different pathological process and as a mechanism contributing to homocysteine (Hcy)-induced hepar injury. However, the molecular event that Hcy-induced ER stress in the hepar under the atherosclerosis background is currently unknown. Endoplasmic reticulum oxidoreductin 1α (ERO1α) plays a crucial ...
Agrawal Ankit A Department of Microbiology, University of Georgia, Athens, GA 30602, - - 2014
Here we report that RNase P is required for the initial separation of all seven valine tRNAs from three distinct polycistronic transcripts (valV valW, valU valX valY lysY and lysT valT lysW valZ lysY lysZ lysQ). Particularly significant is the mechanism by which RNase P processes the valU and lysT ...
Sardina José L JL Department of Biochemistry and Molecular Biology, University of Salamanca, Salamanca, Spain; IBFG, Instituto de Biología Funcional y Genómica, CSIC, Salamanca 37007, - - 2014
PTPN13 is a high-molecular weight intracellular phosphatase with several isoforms that exhibits a highly modular structure. Although in recent years different roles have been described for PTPN13, we are still far from understanding its function in cell biology. Here we show that PTPN13 expression is activated during megakaryocytic differentiation at ...
Gao Chaobing C Department of Otorhinolaryngology, Head and Neck Surgery, First Affiliated Hospital of AnHui Medical University , Hefei , - - 2014
Abstract Conclusions: Increased expression of Dicer may be a prognostic biomarker for patients with laryngeal squamous cell carcinoma (LSCC). Recent studies have shown that many microRNAs (miRNAs) play an important role in the development and progression of human cancers. Dicer, one of the most important enzymes of the miRNA machinery, ...
Petkovic Vibor V Division of Pediatric Endocrinology, Diabetology and Metabolism and Department of Clinical Research, University Children's Hospital, Bern, - - 2014
Background: Aggregation of growth hormone (GH) required for its proper storage in granules is facilitated by zinc (Zn(2+)) transported by specific zinc transporters in and out of the regulated secretory pathway. Slc30a5 (ZnT5) was reported to have the highest gene expression among all zinc transporters in primary mouse pituitary cells ...
Candi E E 1] Biochemistry Laboratory, IDI-IRCCS, Rome 00133, Italy [2] Department of Experimental Medicine and Surgery, University of Rome 'Tor Vergata', Rome 00133, - - 2014
MicroRNAs (miRs) are a class of small noncoding RNAs that suppress the expression of protein-coding genes by repressing protein translation. Although the roles that miRs and the miR processing machinery have in regulating epithelial stem cell biology are not fully understood, their fundamental contributions to these processes have been demonstrated ...
Bavelloni Alberto A SC Laboratory of Musculoskeletal Cell Biology, Rizzoli Orthopedic Institute, Bologna, Italy; Laboratory RAMSES, Rizzoli Orthopedic Institute, Bologna, - - 2014
Here we report that both PLCâ1a and PLCâ1b are relevant regulators of erythropoiesis in that kinamycin F, a potent inducer of γ-globin production in K562 cells, caused a selectively reduction of both PLCâ1 isozymes even though the results point out that the effect of the drug is mainly directed toward ...
Caballero Isabel Martín IM Centre for Integrative Physiology, University of Edinburgh, Edinburgh EH8 9XD, - - 2014
During development, region-specific patterns of regulatory gene expression are controlled by signaling centers that release morphogens providing positional information to surrounding cells. Regulation of signaling centers themselves is therefore critical. The size and the influence of a Shh-producing forebrain organizer, the zona limitans intrathalamica (ZLI), are limited by Pax6. By ...
Komiya Yuko Y Department of Biology, College of Science and Technology, Temple University, Philadelphia, PA - - 2014
Precise control of the canonical Wnt pathway is crucial in embryogenesis and all stages of life, and dysregulation of this pathway is implicated in many human diseases including cancers and birth defect disorders. A key aspect of canonical Wnt signaling is the cytoplasmic to nuclear translocation of β-catenin, a process ...
Gerwig Jan J Department of General Microbiology, Georg-August-University Göttingen, - - 2014
RNA degradation is a major mechanism of post-transcriptional control of gene expression. Moreover, the rapid turnover of bacterial mRNAs is central to the fast adaptation of these organisms to changing environmental conditions by the regulation of transcription initiation. In most bacteria, RNA processing and degradation require the concerted action of ...
Braun Katherine A KA Department of Biochemistry, University of Washington, Seattle, - - 2014
Post-transcriptional regulation of gene expression has an important role in defining the phenotypic characteristics of an organism. Well-defined steps in mRNA metabolism that occur in the nucleus - capping, splicing, and polyadenylation - are mechanistically linked to the process of transcription. Recent evidence suggests another link between RNA polymerase II ...
Pan Yuan Y Department of Biochemistry, Carver College of Medicine, University of Iowa, 51 Newton Road, Biochemistry, 4-712 BSB, Iowa City, IA, 52242, - - 2014
Hyperpolarization-activated cyclic nucleotide-gated 1 (HCN1) channels carry Ih, which contributes to neuronal excitability and signal transmission in the nervous system. Controlling the trafficking of HCN1 is an important aspect of its regulation, yet the details of this process are poorly understood. Here, we investigated how the C-terminus of HCN1 regulates ...
Margarit Ezequiel E Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) - Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Ocampo y Esmeralda, (S2000FHQ) Rosario, - - 2014
Cellular nucleic acid binding protein (CNBP) is a small and highly conserved protein with nucleic acid chaperone activity that binds single-stranded nucleic acids. Data collected so far suggests that CNBP is required for proper craniofacial development. Despite the advances achieved in the last decade, the identity of the molecular targets ...
Yu Jianshe J School of Mathematics and Information Science, Guangzhou University, Guangzhou, 510006, China; Key Laboratory of Mathematics and Interdisciplinary Sciences of Guangdong Higher Education Institutes, Guangzhou University, Guangzhou, 510006, China. Electronic address: - - 2014
Gene transcription is a stochastic process, and is often activated by multiple signal transduction pathways. In this work, we study gene transcription activated randomly by two cross-talking pathways, with the messenger RNA (mRNA) molecules being produced in a simple birth and death process. We derive the analytical formulas for the ...
Perez-Martin Marta M CSIC-Universidad de - - 2014
The accumulation of unfolded/misfolded proteins in the endoplasmic reticulum (ER) results in the activation of stress responses such as the unfolded protein response or the catabolic process of autophagy to ultimately recover cellular homeostasis. ER stress also promotes the production of reactive oxygen species (ROS), which play an important role ...
Dai Wei-Ping WP Department of Gastroenterology, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, - - 2014
Epithelial-mesenchymal transition (EMT) process and extracellular signal-regulated kinase 1 (ERK1) signaling pathway play pivotal roles in hepatic stellate cell (HSC) activation, which is associated with the altered expression patterns of microRNAs (miRNAs). miR-155 is considered a typical multifunctional miRNA to regulate many biological processes. However, little attention has been given ...
Peng Xiao Hong - - 2014
The molecular mechanisms underlying dysregulation of microRNAs have been documented in nasopharyngeal carcinoma (NPC). Our previous study demonstrated that plasma miR-124 was down-regulated in NPC using microarray analysis and quantitative PCR validation. Though growing studies showed that down-regulated miR-124 was closely related to tumourigenesis in various types of cancers, the ...
Sladewski Thomas - - 2014
mRNA localization ensures correct spatial and temporal control of protein synthesis in the cell. We show that an in vitro single molecule approach, using purified recombinant full-length proteins and synthesized mRNA, provides insight into the mechanism by which localizing mRNAs are carried to their destination. A messenger ribonucleoprotein (mRNP) complex ...
Sagomonyants Karen K Department of Craniofacial Sciences, School of Dental Medicine, University of Connecticut Health Center , Farmington, CT , - - 2014
Abstract Odontoblast differentiation during physiological and reparative dentinogenesis is dependent upon multiple signaling molecules, including fibroblast growth factors (FGFs), bone morphogenetic proteins (BMPs) and Wingless/Integrated (Wnt) ligands. Recent studies in our laboratory showed that continuous exposure of primary dental pulp cultures to FGF2 exerted biphasic effects on the expression of ...
Stoll Birgit - - 2014
Post-transcriptional maturation of plant mitochondrial transcripts requires several steps. Among these, the generation of mature 5' ends is still one of the most enigmatic processes. Toward a characterization of proteins involved in 5' processing of mitochondrial transcripts in Arabidopsis (Arabidopsis thaliana), we now analyzed 5' maturation of nad2 transcripts. Based ...
Xu Donghua D Department of Rheumatology, the First Affiliated Hospital, Nanjing Medical University, 210029, Nanjing, - - 2014
Protein tyrosine phosphatase receptor type O (PTPRO) has been identified as a tumor suppressor in a number of cancers including hepatocellular carcinoma (HCC). Toll-like receptor 4 (TLR4) plays diverse roles in HCC tumorigenesis and progression. The association between PTPRO and TLR4 signaling in HCC remains largely unknown. We aimed to ...
Poi Ming J MJ Department of Pharmacy, The Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, Columbus, OH, U.S.A Division of Pharmacy Practice and Administration, College of Pharmacy, The Ohio State University, Columbus, OH, - - 2014
Background/Aim: While aberrant expression of cyclin-dependent kinase-4 (CDK4) has been found in squamous cell carcinoma of the head and neck (SCCHN), the associations between CDK4 and its regulators, namely, cyclin D1, cyclin E, gankyrin, SEI1, and BMI1 in gene expression remain to be explored. Herein we investigated the mRNA profiles ...
Giesen Eva E Yale Cancer Center, Yale University School of Medicine, New Haven, CT, U.S. These authors contributed equally to this - - 2014
Background: Novel immune therapies targeting tumor specific antigens are being developed. Our purpose was to determine expression of the cancer testes antigen NY-ESO-1 in renal cell carcinoma (RCC), as NY-ESO-1 targeting approaches, particularly adoptive cell therapy, have not been evaluated in this disease. Methods: We employed tissue microarrays containing >300 ...
Miyoshi Kina K Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan; Department of Pediatric Surgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, - - 2014
CXC chemokine receptor 4 (CXCR4) expression is reportedly correlated with both vascular endothelial growth factor (VEGF) expression and poor prognosis in a variety of cancers. Its relation to CXC chemokine receptor 7 (CXCR7) is also noted in several malignancies, including rhabdomyosarcoma (RMS) cell lines. However, the correlations between these chemokine ...
Liu Zhiyong Z Department of Pathology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, China; The Key Laboratory of Tianjin Cancer Prevention and Treatment, Tianjin, 300060, China; National Clinical Research Center for Cancer, Tianjin, 300060, - - 2014
Dickkopf-1 (Dkk1), an antagonist of wnt/β-catenin signaling pathway, has been reported to play a role in cancer progression. However, little is known about the role of Dkk1 during colorectal adenoma-carcinoma sequence. This study aimed to elucidate the role of Dkk1 in tumorigenesis and angiogenesis in colorectal cancer. we examined Dkk1 ...
Wang Lei - - 2014
The introduction of cisplatin has improved the long-term survival rate in osteosarcoma patients. However, some patients are intrinsically resistant to cisplatin. This study reported that the activation of Notch1 is positively correlated with cisplatin sensitivity, evidenced by both clinical and in vitro data. In this study, a total 8 osteosarcoma ...
Feng Ai-Ping AP Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China, - - 2014
The deubiquitinating enzyme ubiquitin specific peptidase 15 (USP15) is regarded as a regulator of TGFβ signaling pathway. This process depends on Smad7, the inhibitory factor of the TGFβ signal, and type I TGFβ receptor (TβR-I), one of the receptors of TGFβ. The expression level of USP15 seems to play vital ...
Vieten D D Department of Paediatric Surgery, Bristol Royal Hospital for Children, Bristol, UK Mucin Research Group, University of Bristol, Bristol, - - 2014
The pivotal role of epidermal growth factor receptor (EGFR) in transducing signalling by multiple ligands involved in proliferation, differentiation and migration is evidenced by the fact that EGFR-null mice invariably die in utero or early post-natal life. Inactivation of EGFR in knockout mice results in a haemorrhagic enterocolitis similar to ...
Vieten D D Department of Paediatric Surgery, Bristol Royal Hospital for Children, Bristol, UK Mucin Research Group, University of Bristol, Bristol, - - 2014
The aetiology of necrotizing enterocolitis (NEC) is multifactorial and its pathogenesis poorly understood. The trefoil factor peptides (TFF1-3) contribute to protective mechanisms operating in the gastro-intestinal (GI) mucosa and play a fundamental role in epithelial protection, restitution and repair. The role of TFF1-3 in neonatal mucosal protection has not been ...
Shin Su-Jin SJ Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, - - 2014
Multiple myeloma (MM) is a heterogeneous and ultimately fatal disease. Risk stratification using prognostic biomarkers is crucial to individualize treatments. We sought to investigate the role of CD99, a transmembrane protein highly expressed in many hematopoietic cells including subpopulations of normal and neoplastic plasma cells, for MM risk stratification. CD99 ...
Ahn Ei Yong - - 2014
Telomere dysfunction is important in carcinogenesis, and recently, stathmin and elongation factor 1alpha (EF1alpha) were reported to be up-regulated in telomere dysfunctional mice. In the present study, the expression levels of stathmin and EF1alpha in relation to telomere length, telomere dysfunction-induced foci (TIF), gamma-H2AX, and p21WAF1/CIP1 expression were assessed in ...
Moura Caio M CM Laboratory of Medical Investigation (LIM 55), University of Sao Paulo Medical School, Sao Paulo - - - 2014
CD44 is a transmembrane glycoprotein and is regarded as a potential marker in various tumors. The aim of our study was to analyze the expression of the standard form of CD44 (CD44s) and its isoforms in localized prostate cancer (PCa), and to correlate these data with the classical prognostic factors ...
Davidson Ben B Department of Pathology, Oslo University Hospital, Norwegian Radium Hospital, N-0424, Oslo, Norway, - - 2014
The objective of this study was to investigate the expression and clinical role of the spindle checkpoint kinase budding uninhibited by benzimidazole 1 (Bub1) in primary and metastatic advanced-stage ovarian serous carcinoma. BUB1 mRNA expression was analyzed in 178 tumors (88 effusions, 38 primary carcinomas, and 52 solid metastases) from ...
Wang D - - 2014
Purpose : The objective of this study was to develop a sensitive, specific and rapid approach to diagnose hand foot and mouth disease (HFMD) for an early treatment by using loop-mediated isothermal amplification (LAMP) technique. Materials and Methods : A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) for detecting EV71 virus was ...
Mazibrada Jasenka J Department of Oncology, University of Turin at San Luigi Hospital, Turin, - - 2014
The aim of this study was to analyze protein and gene expression of HER2 in 224 head and neck precancerous and malignant lesions by immunohistochemistry and FISH analysis. In parallel, expression of pStat3, Sox2, IFI16 and p16, Ki67 was evaluated. Immunohistochemical analysis was assessed on formalin-fixed paraffin-embedded (FFPE) tissue specimens. ...
Wang Liang L Department of Basic Medicine, School of Medicine, Shenzhen University, Shenzhen, Guangdong 518060, P.R. - - 2014
The aim of the current study was to investigate the expression of cyclin‑dependent kinase 3 (CDK3) in human nasopharyngeal carcinoma (NPC) and to evaluate its association with the clinicopathological characteristics of patients with NPC. CDK3 expression was examined in three NPC cell lines and one nasopharyngeal epithelial cell line by western blot analysis ...
Carrarelli Patrizia P Department of Molecular and Developmental Medicine, University of Siena, Siena, - - 2014
To evaluate antimüllerian hormone (AMH) and AMH receptor II (AMHRII) mRNA and protein expression in endometrium and in ovarian or deep lesions of women with endometriosis. Prospective study. University hospitals in Italy and Brazil. Patients with endometriosis (n = 55) and healthy women (n = 45). Specimens of endometrium obtained by hysteroscopy from patients ...
Janusicova Veronika V Departments of 1Obstetrics and Gynecology and 2Molecular Biology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovak - - 2014
The objective of current study was to determine the p16 mRNA level in cervical cells by relative quantification (RQ) and to test viral E6 expression in human papillomavirus (HPV) -16 or -18-positive specimens by widely used methods. We targeted the pivotal mRNA level associated with severe dysplasia or worse. Cervical ...
Tang Shao Hui SH Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, - - 2014
Hepatitis B virus (HBV) X protein (HBx) contributes to hepatocarcinogenesis. The overexpression of transcripts from P3 and P4 promoters of the insulin-like growth factor 2 (IGF2) gene is observed in hepatocellular carcinoma (HCC). Here we aimed to explore the involvement of HBx in P3-driven mRNA overexpression and underlying epigenetic mechanism. ...
Li Tianhong T *University of California Davis Comprehensive Cancer Center, †Department of Internal Medicine, Division of Hematology & Oncology, University of California, Davis, School of Medicine, Sacramento, California; ‡Department of General-, Visceral- and Tumor Surgery, University of Cologne, Cologne, Germany; §Response Genetics, Inc., Los Angeles, California; ‖Department of Public Health Sciences, University of California, Davis, Davis, California; ¶Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California; and #formerly Response Genetics, Inc., Los Angeles, - - 2014
The objective of this study was to identify and characterize echinoderm microtubule-associated protein-like 4 anaplastic lymphoma kinase fusion (EML4-ALK+) cancers by variant-specific, quantitative reverse transcription polymerase chain reaction (RT-PCR) assays in a large cohort of North American non-small-cell lung cancer (NSCLC) patients. We developed a panel of single and multiplex ...
Nobusawa Aiko A Department of Diagnostic Pathology, Gunma University Graduate School of Medicine, Maebashi, Japan; Department of Stomatology and Maxillofacial Surgery, Gunma University Graduate School of Medicine, Maebashi, - - 2014
Diagnosis of the exact grade of oral epithelial dysplasia is difficult, and interobserver variations in grading are common. The aim of this study was to investigate the expression patterns of cytokeratins (CKs) in dysplastic oral epithelia, to identify useful double immunostaining diagnostic markers. Immunoexpression of CK13, CK14, CK17, and Ki-67 ...
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