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Dupnik Kathryn M KM Division of Infectious Diseases and Center for Global Health, Weill Cornell Medical College, New York, NY, 10021, - - 2014
Leprosy morbidity is increased by two pathologic immune reactions, reversal reaction (RR) and erythema nodosum leprosum (ENL). To discover host factors related to immune reactions, global transcriptional profiles of PBMC were compared between 11 RR, 11 ENL, and 19 matched control patients, with confirmation with qPCR. Encoded proteins were investigated ...
Lo Vasco Vincenza Rita VR Department of Sense Organs, Sapienza University, Rome, Italy - - 2014
Ezrin, a member of the ezrin-radixin-moesin family, is involved in the metastatic spread of osteosarcoma. Ezrin binds phosphatydil inositol-4,5-bisphosphate (PIP2), a crucial molecule of the phosphoinositide signal transduction pathway. PIP2 levels are regulated by phosphoinositide-specific phospholipase C (PI-PLC) enzymes. PI-PLCε isoform, a well-characterized direct effector of rat sarcoma (RAS), is ...
Lo Vasco V R VR Sense Organs Department, Policlinico Umberto I Faculty of Medicine and Dentistry, Sapienza University of Rome, viale del Policlinico 155, 00185, Italy, Rome, - - 2014
Ezrin, a protein belonging to the Ezrin, radixin and moesin (ERM) family, was engaged in the metastatic spread of osteosarcoma. The Protein 4.1, Ezrin, radixin, moesin (FERM) domain of Ezrin binds the membrane Phosphatydil inositol (4,5) bisphosphate (PIP2), a crucial molecule belonging to the Phosphoinositide (PI) signal transduction pathway. The ...
Xi Xin X Department of Biochemistry, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, - - 2014
The class I phosphoinositide 3-kinase (PI3K) can be activated by a large variety of extracellular stimuli and is responsible for generating phosphatidylinositol-3, 4, 5-trisphosphate (PI(3,4,5)P3) from phosphatidylinositol-4, 5-bisphosphate at the plasma membrane. The expression pattern of the class I PI3K and distribution of PI(3,4,5)P3, visualized by its specific binding protein, ...
Gou Lan-Tao LT 1] Center for RNA Research, State Key Laboratory of Molecular Biology-University of Chinese Academy of Sciences, Shanghai 200031, China [2] Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, - - 2014
Spermatogenesis in mammals is characterized by two waves of piRNA expression: one corresponds to classic piRNAs responsible for silencing retrotransponsons and the second wave is predominantly derived from nontransposon intergenic regions in pachytene spermatocytes, but the function of these pachytene piRNAs is largely unknown. Here, we report the involvement of ...
Yao Zhu-Fang ZF State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Root Biology Center, South China Agricultural University, Guangzhou 510642, PR - - 2014
Proteins containing the SPX domain are believed to play vital roles in the phosphorus (P) signalling network in plants. However, the functions of SPX proteins in legumes remain largely unknown. In this study, three SPX members, PvSPX1-PvSPX3 were cloned from common bean (Phaseolus vulgaris L.). It was found that the ...
Wang Dennis Ding-Hwa DD University of California, Irvine, United - - 2014
Mammalian mitochondrial mRNA (mt-mRNA) transcripts are polyadenylated at 3' end with different lengths. The SUV3/PNPase complex and mtPAP have been shown to degrade and polyadenylate mitochondrial mRNA, respectively. How these two opposite actions are coordinated to modulate mt-mRNA poly(A) lengths is of interest to pursue. Here, we demonstrated that a ...
Qundan Lv L State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, People's Republic of - - 2014
PHR2, a central regulator of phosphate signaling in rice, enhanced the expression of phosphate starvation-induced (PSI) genes and resulted in the enhancement of Pi acquisition under Pi deficiency stress. This occurred via PHR2 binding to a cis-element named the PHR1 binding sequences. However, the transcription level of PHR2 was not ...
Kang Baoshan B Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, China Agricultural University, No. 2 Yuanmingyuan Xilu, Beijing, 100193, - - 2014
Carotenoid content is the primary determinant of fruit color that affects nutritional value and appearance in tomato. Phytoene synthase (PSY) is the key regulatory enzyme in the carotenoid biosynthesis pathway. Absent function of PSY1 in tomato fruit results in yellow flesh phenotype. We, here, report that two different transcripts, a ...
Yang Won Tae WT College of Life Science and Natural Resources, Dong-A University, Busan 604-714, Republic of - - 2014
R2R3 MYB transcription factors play regulatory roles in plant responses to various environmental stresses and nutrient deficiency. In this study, we isolated and designated OsMYB4P, an R2R3 MYB transcription factor, from rice (Oryza sativa L. 'Dongjin') under phosphate-deficient conditions. OsMYB4P was localized in the nucleus and acted as a transcriptional ...
Liu Dong D The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR - - 2014
Benzo[a]pyrene (B[a]P) has a high carcinogenic potential. B[a]P concentrations and molecular biomarkers (mRNA expressions of Pgp, AhR, CYP4, CYP414A1, GST-pi, GST-S2, Cu/Zn-SOD and Mn-SOD) were assayed in gills and digestive glands of the clam Ruditapes philippinarum exposed to 0.03, 0.3 and 3 μg/L B[a]P for 21 days and then exposed to ...
Wang Hui H China Agricultural - - 2014
The WRKY transcription factor family has more than 70 members in the Arabidopsis genome, and some of them are involved in plant responses to biotic and abiotic stresses. This study evaluated the role of WRKY45 in regulating phosphate (Pi) uptake in Arabidopsis. WRKY45 was localized in the nucleus and mainly ...
Davis Shaun M SM 1] Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA - - 2014
Aggressive behaviour is widespread throughout the animal kingdom. However, its mechanisms are poorly understood, and the degree of molecular conservation between distantly related species is unknown. Here we show that knockdown of tailless (tll) increases aggression in Drosophila, similar to the effect of its mouse orthologue Nr2e1. Tll localizes to ...
Takagi K K Department of Health and Nutritional Science, Faculty of Human Health Science, Matsumoto University, Matsumoto, Nagano-, - - 2014
The rat enhancer of split- and hairy-related protein-1 (SHARP-1) is a basic helix-loop-helix transcription factor. An issue of whether SHARP-1 is an insulin-inducible transcription factor was examined. Insulin rapidly increased the level of SHARP-1 mRNA both in vivo and in vitro. Then, signaling pathways involved with the increase of SHARP-1 ...
Ramaiah Madhuvanthi M 1 Purdue - - 2014
Phosphate (Pi) availability is a major factor determining growth and consequently the productivity of crops. However it is one of the least available macronutrient due to its high fixation in the rhizosphere. To overcome this constraint, plants have developed adaptive responses to better acquire, utilize and recycle Pi. Molecular determinants ...
Li X H XH North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Department of Plant Genetics and Breeding, Agricultural University of Hebei, Baoding, Hebei, - - 2014
Phosphorus plays a pivotal role in plant growth and development. In this study, we isolated and characterized GmPTF1, a basic helix-loop-helix (bHLH) transcription factor (TF) gene from soybean (Glycine max) with tolerance to inorganic phosphate (Pi) starvation. Alignment analysis indicated that GmPTF1 and other reported bHLH TFs share significant similarity ...
Xiong Jie J Department of Statistics, College of Mathematics and Computer Science, Hunan Normal University, Changsha, China; Department of Molecular Epidemiology, College of Medicine, Hunan Normal University, Changsha, - - 2014
Although patients with Glioblastoma multiforme (GBM) have grave prognosis, significant variability in patient outcome is observed. The objective of this study is to identify a molecular signature for GBM prognosis. We subjected 355 mRNA and microRNA expression profiles to elastic net-regulated Cox regression for identification of an integrated RNA signature ...
Gallarato Lucas A - - 2013
The exopolyphosphatase (Ppx) of Pseudomonas aeruginosa is encoded by PA5241 gene (ppx). Ppx catalyzes the hydrolysis of inorganic polyphosphates (polyPs) to orthophosphate (Pi). In the present work we identified and characterize the promoter region of ppx and its regulation under environmental stress conditions. The role of Ppx in the production ...
Secco David - - 2013
Using rice (Oryza sativa) as a model crop species, we performed an in-depth temporal transcriptome analysis, covering the early and late stages of Pi deprivation as well as Pi recovery in roots and shoots, using next-generation sequencing. Analyses of 126 paired-end RNA sequencing libraries, spanning nine time points, provided a ...
Lo Vasco Vincenza Rita - - 2013
Besides the control of calcium levels, the phosphoinositide-specific phospholipases C (PI-PLCs), the main players in the phosphoinositide signalling pathway, contribute to a number of cell activities. The expression of PI-PLCs is strictly tissue specific and evidence suggests that it varies under different conditions, such as tumour progression or cell activation. ...
Deshpande Deepak A DA 2Department of Medicine, Thomas Jefferson University, Room 317, College Bldg., 1025 Walnut St., Philadelphia, PA 19107, USA. - - 2014
To clarify the potential utility of targeting GRK2/3-mediated desensitization as a means of manipulating airway smooth muscle (ASM) contractile state, we assessed the specificity of GRK2/3 regulation of procontractile and relaxant G-protein-coupled receptors in ASM. Functional domains of GRK2/3 were stably expressed, or siRNA-mediated GRK2/3 knockdown was performed, in human ...
Aziz Tariq - - 2013
Recent studies have identified genotypic variation in phosphorus (P) efficiency, but rarely have the underlying mechanisms been described at the molecular level. We demonstrate that the highly P-efficient wheat (Triticum aestivum L.) cultivar Chinese 80-55 maintains higher inorganic phosphate (Pi ) concentrations in all organs upon Pi withdrawal in combination with ...
Zhu Li L From the Department of Neurology and the Alzheimer's Disease Research Center - - 2013
Recent studies link synaptojanin 1 (synj1), the main phosphoinositol (4,5)-biphosphate phosphatase (PI(4,5)P2-degrading enzyme) in the brain and synapses, to Alzheimer disease. Here we report a novel mechanism by which synj1 reversely regulates cellular clearance of amyloid-β (Aβ). Genetic down-regulation of synj1 reduces both extracellular and intracellular Aβ levels in N2a ...
Rojas Hernán J HJ Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC-CONICET), Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, X5000HUA, Córdoba, - - 2013
Non-S-ribonucleases (non-S-RNases) are class III T2 RNases constitutively expressed in styles of species with S-RNase-based self-incompatibility. So far, no function has been attributed to these RNases. The aim of this work is to examine if NnSR1, a non-S-RNase from Nicotiana alata, is induced under conditions of phosphate (Pi) deprivation. The ...
Garcia Kevin - - 2013
Mycorrhizal exchange of nutrients between fungi and host plants involves a specialization and polarization of the fungal plasma membrane adapted for the uptake from the soil and for secretion of nutrient ions towards root cells. In addition to the current progress in identification of membrane transport systems of both symbiotic ...
Azechi Takuya - - 2013
Aim: Vascular calcification, a major complication of chronic kidney disease (CKD), refers to the mineralization of vascular smooth muscle cells (VSMCs), resulting from a phenotypic change towards osteoblast-like cells. Histone deacetylase inhibitors (HDIs), potential therapeutic agents for CKD, are known to promote the differentiation and mineralization of osteoblasts. In this ...
Liu Qianhe - - 2013
Alkaline phosphatases (ALP) in arbuscular mycorrhizal (AM) fungi have been suggested to be involved in transfer of phosphate from the mycorrhizal fungus to the host plant, but exact mechanisms are still unknown, partially due to the lack of molecular information. We isolated a full-length cDNA (FmALP) from the AM fungus ...
Yan Xiaoling - - 2013
CD4 protein is a single chain transmembrane glycoprotein belonging to the immunoglobin superfamily that recognize the major histocompatibility complex (MHC) class II molecules. It plays an important role in cell-mediated immunity. Here, the full-length cDNA of CD4 in Chinese goose (Anser cygnoides) was cloned and identified. The goose CD4 is ...
Ciceri Paola - - 2013
Vascular calcification (VC) represents a major cardiovascular risk factor in chronic kidney disease patients. High phosphate (Pi) levels are strongly associated with VC in this population. Therefore, Pi binders are commonly used to control high Pi levels. The aim of this work was to study the mechanism of action of ...
Spina Annamaria - - 2013
Inorganic phosphate (Pi) is an essential nutrient to living organisms. It plays a key role in diverse biological processes, including osteoblast differentiation and skeletal mineralization. Maintenance of proper Pi homeostasis is a critical event, as any deviation from that state can lead to several acute and chronic disease states and ...
Baek Dongwon - - 2013
In plants, microRNA399 (miR399) is a major regulator of phosphate (Pi) homeostasis by way of post-transcriptional mechanisms including transcript cleavage and transcriptional repression. Although miRNA genomic organization, biogenesis, and mode of action in plants are known, the regulatory mechanisms affecting miRNAs are poorly understood. We have shown that AtMYB2 functions ...
Michigami Toshimi - - 2013
Phosphorus is involved in various biological processes including membrane integrity, maintenance and inheritance of genetic materials, energy metabolism, intracellular signaling and skeletal mineralization. In addition, accumulating evidences have indicated that alteration in the levels of extracellular inorganic phosphate (Pi) itself triggers signaling to regulate gene expression and cellular functions in ...
Zhao Ningbo - - 2013
Period1 (PER1) is an important core clock gene, which regulates normal cell proliferations and physiological rhythms of human beings. Recent studies have showed aberrant expressions and altered rhythms of PER1 were highly correlated to the carcinogenesis and development of malignant tumors. However, there is no study on the correlation of ...
Fernández-Sáiz Vanesa - - 2013
The Tel2 (also known as Telo2) and Tti1 proteins control the cellular abundance of mammalian PIKKs and are integral components of mTORC1 and mTORC2. Here we report that Tel2 and Tti1 are targeted for degradation within mTORC1 by the SCFFbxo9 ubiquitin ligase to adjust mTOR signalling to growth factor availability. ...
Son Bo-Kyung - - 2012
Hyperphosphatemia has emerged as a cardiovascular risk factor that stimulates calcification in vessels. We explored molecules that were induced by inorganic phosphate (Pi) at an early stage in vascular smooth muscle cells (VSMC). In the present study, we examined the role of thrombomodulin (TM) in Pi-induced VSMC calcification based on ...
Ito Nobuaki - - 2012
1α,25-dihydroxy vitamin D(3) (1,25D) is reported to up-regulate the expression of the osteocyte-derived phosphatonin, fibroblast growth factor 23 (FGF23), an effect increased by high concentrations of extracellular phosphate (Pi). Osteocytes therefore appear to sense Pi directly and this may be an important means, by which FGF23 production is regulated. The ...
Fang Rejun R College of Animal Science, Henan Institute of Science and Technology, Xinxiang, Henan Province 453003, China - - 2012
Intestinal phosphate (Pi) absorption across the apical membrane of small intestinal epithelial cells is mainly mediated by the type IIb Na-coupled phosphate co-transporter (NaPi-IIb), but its expression and regulation in the chicken remain unclear. In the present study, we investigated the mRNA and protein levels of NaPi-IIb in three regions ...
Kajiwara Mamiko - - 2012
Fcγ receptors I and III are thought to be involved in the development of lupus nephritis. Expression of Fc receptor common gamma chain (FcRr) is necessary for the stable expression of Fcγ receptors I and III. The aim of this study was to develop a novel agent for the treatment ...
Taliaz Dekel - - 2012
BACKGROUND: Impaired neuronal plasticity and, specifically, altered expression of brain-derived neurotrophic factor (BDNF) were shown to play a critical role in depressive behavior and the mechanism of various antidepressant treatments including electroconvulsive therapy (ECT). Interestingly, opposing roles were suggested for BDNF in the hippocampus and the ventral tegmental area (VTA), ...
Turpin E - - 2012
Background and purpose:  Carbamazepine, known for its anti-epileptic, analgesic and mood-stabilizing properties, is known to induce weight gain but the pathophysiology of this adverse event is still largely unknown. We tested the hypothesis that carbamazepine could have a direct effect on adipocyte development and metabolism. Experimental research:  we studied the ...
Venø Morten T - - 2012
Editing by ADAR enzymes is essential for mammalian life. Still, knowledge of the spatio-temporal editing patterns in mammals is limited. By use of 454 amplicon sequencing we examined the editing status of 12 regionally extracted mRNAs from porcine developing brain encompassing a total of 64 putative ADAR editing sites. In ...
Li Yan - - 2012
Endocannabinoid (eCB) signaling serves as an on-demand neuroprotective system. eCBs are produced postsynaptically in response to depolarization or activation of metabotropic glutamate receptors (mGluRs) and act on presynaptic cannabinoid receptor-1 to suppress synaptic transmission. Here, we examined the effects of epileptiform activity on these two forms of eCB signaling in ...
Tanaka Miyabi M Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California 90095-1735, - - 2012
The β3 subunit of the γ-aminobutyric acid type A receptors (GABA(A) -Rs) is an essential component of GABA(A) -Rs in fetal, perinatal, and adult mammalian brain. Various transcripts of the β3 subunit gene (GABRB3) produce various proteins with different N-termini. Rare variants in this N-terminus (exon 1A and exon 2) ...
Peng Yu-Fen - - 2012
This review discusses the experimental and clinical studies those show the expression of connexin 36 in the central nervous system and the possible role of connexin 36 in epileptic seizure. All articles used in this review were mainly searched from PubMed published in English from 1996 to 2012. Original articles ...
Jimenez-Mateos Eva M - - 2012
Temporal lobe epilepsy is a common, chronic neurological disorder characterized by recurrent spontaneous seizures. MicroRNAs (miRNAs) are small, noncoding RNAs that regulate post-transcriptional expression of protein-coding mRNAs, which may have key roles in the pathogenesis of neurological disorders. In experimental models of prolonged, injurious seizures (status epilepticus) and in human ...
Huang Xuan X The Graduate Program of Neuroscience, Vanderbilt University Medical Center, Nashville, TN 37212, - - 2012
The GABRG2 nonsense mutation, Q40X, is associated with the severe epilepsy syndrome, Dravet syndrome, and is predicted to generate a premature translation-termination codon (PTC) in the GABA(A) receptor γ2 subunit mRNA in a position that codes for the first amino acid of the mutant subunit. We determined the effects of ...
Baxendale Sarah S MRC Centre for Developmental and Biomedical Genetics, Department of Biomedical Science, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, - - 2012
The availability of animal models of epileptic seizures provides opportunities to identify novel anticonvulsants for the treatment of people with epilepsy. We found that exposure of 2-day-old zebrafish embryos to the convulsant agent pentylenetetrazole (PTZ) rapidly induces the expression of synaptic-activity-regulated genes in the CNS, and elicited vigorous episodes of ...
Tao Shuxin - - 2012
Methyl CpG binding protein-2 (MeCP2) is a multifunctional nuclear protein, and regulates dendritic morphology, synaptic transmission, spontaneous neurotransmission, and short-term synaptic plasticity in the central nervous system. This study was designed to investigate the expression of MeCP2 mRNA and protein in intractable temporal lobe epilepsy (TLE) patients and an experimental ...
Neves Guilherme - - 2012
Deletion of LIM homeodomain transcription factor-encoding Lhx6 gene in mice results in defective tangential migration of cortical interneurons and failure of differentiation of the somatostatin (Sst)- and parvalbumin (Pva)-expressing subtypes. Here, we characterize a novel hypomorphic allele of Lhx6 and demonstrate that reduced activity of this locus leads to widespread ...
Cunningham James K - - 2012
BACKGROUND: Clandestine laboratory operators commonly extract ephedrine and pseudoephedrine-precursor chemicals used to synthesize methamphetamine-from over-the-counter cold/allergy/sinus products. To prevent this activity, two states, Oregon in 07/2006 and Mississippi in 07/2010, implemented regulations classifying ephedrine and pseudoephedrine as Schedule III substances, making products containing them available by prescription only. Using simple ...
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