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Gyurkovska Valeriya V Department of Immunology, Institute of Microbiology , Sofia , Bulgaria - - 2014
Abstract Context: Osteoarthritis (OA) has become by far the most common joint disorder. A number of studies using OA animal models have explored the effects of agents that can modulate bone metabolism. Objective: In the present study, we investigated the effect of acetylated derivative of plant alkaloid glaucine (ADG) on ...
Chen Shih-Yao SY Department of Internal Medicine, National Cheng Kung University Medical College, Tainan, Taiwan; Department of Biochemistry and Molecular Biology, National Cheng Kung University Medical College, Tainan, - - 2014
Objective. Transcription factor Snail is involved in various biological functions. We hypothesized that this molecule regulates TNF-α-mediated synovial fibroblasts (SF) activation in rheumatoid joint, and examined its roles in expression of Cadherin-11 (Cad-11) and myofibroblast markers, invasive ability and IL-6 production. Methods. Synovium was obtained from rheumatoid arthritis (RA) patients ...
Plotnik Joshua P JP Department of Biology, Indiana University, Bloomington, Indiana, - - 2014
The RAS/ERK pathway is commonly activated in carcinomas and promotes oncogenesis by altering transcriptional programs. However, the array of cis-regulatory elements and trans-acting factors that mediate these transcriptional changes is still unclear. Our genome-wide analysis determined that a sequence consisting of neighboring ETS and AP-1 transcription factor binding sites is ...
White Andrew Jr AJ Save Sight Institute, University of Sydney, Sydney, Australia Westmead Millennium Institute, University of Sydney, Sydney, Australia - - 2014
The purpose of this study was to determine the relative expression of clinically-relevant components of the renin-angiotensin system (RAS) in the adult human eye. We obtained 14 post-mortem enucleated human eyes from patients whom had no history of inflammatory ocular disease nor pre-mortem ocular infection. We determined the gene expression ...
Duan Hongmei H Department of Rheumatology and Immunology, the 1st Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, - - 2014
Rheumatoid arthritis (RA) is a systemic inflammatory disease. C-C chemokine receptor type 5 (CCR5) is found in inflamed synovium of RA patients and is necessary for formation of RA. We aimed to check whether delivery of CCR5-specific small interfering RNA (siRNA) via electroporation suppresses local inflammation in arthritis rats. Vectors ...
Oh J S JS University of Ulsan College of Medicine, Department of Orthopedic Surgery, Ulsan University Hospital, 290-3 Jeonha-dong, Dong-gu, Ulsan 682-714, - - 2014
Previous studies support the important role of vascular endothelial growth factor (VEGF) and syndecan-4 in the pathogenesis of osteoarthritis (OA). Both VEGF and syndecan-4 are expressed by chondrocytes and both are involved in the regulation of matrix metalloproteinase-3, resulting in the activation of aggrecanase II (ADAMTS-5), which is essential in ...
Córdoba Sergio S Departamento de Biología Molecular and Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid (UAM), Madrid, - - 2014
A characteristic of all arthropods is the presence of flexible structures called joints that connect all leg segments. Drosophila legs include two types of joints: the proximal or "true" joints that are motile due to the presence of muscle attachment and the distal joints that lack musculature. These joints are ...
Ohrnberger Stefan S Department for Molecular Biology, Tuebingen University, - - 2014
The ubiquitously expressed transcriptional regulator Serum Response Factor (SRF) is controlled by both Ras/MAPK and Rho/actin signaling pathways, which are frequently activated in hepatocellular carcinoma (HCC). We generated SRF-VP16(iHep) mice, which conditionally express constitutively active SRF-VP16 in hepatocytes, thereby controlling subsets of both Ras/MAPK- and Rho/actin-stimulated target genes. All SRF-VP16(iHep) ...
Wan Qian Q The Second Affiliated Medical College and Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 610041, People's Republic of - - 2014
4Retinoic acid (RA) and oleanolic acid (OA) were studied about their potential to induce mouse embryonic stem cell R1/E (MESC-R1/E) to differentiate toward germ cells. Embryoid bodies (EBs) first formed from MESC-R1/E and EBs were allowed to attach to the bottoms of normal cell-culturing plate and grow. Then, different compounds ...
Shimomura Tadahiro T Department of Oral and Maxillofacial Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan. Electronic address: - - 2014
Expression of the LIM homeodomain transcription factor Lhx8 is restricted to and up-regulated in the mesenchyme of the upper face prominence before lip fusion. Msx1/2 acts in early development to control cell proliferation and differentiation. Deficiency of these genes is associated with nonsyndromic cleft lip with/without cleft palate. Since retinoid is ...
Cuffe J S M JS School of Biomedical Sciences, The University of Queensland, St Lucia, Queensland 4072, - - 2014
The renin angiotensin system (RAS) is an important mediator of placental development. However, a comprehensive expression profile for 8 key components of the placental RAS throughout murine gestation has not been performed. Furthermore, maternal hypoxia induces dysregulation of RAS expression in fetal tissues but the effects on the murine placental ...
Trzybulska Dorota D Department of Rheumatology and Clinical Immunology, Poznan University of Medical Sciences, Przybyszewskiego Street 39, 60-356 Poznan, Poland. Electronic address: - - 2014
In rheumatoid arthritis (RA) activity of serine proteases is an important factor contributing to destructive changes in the joints. The aim of this study was to compare elastase (ELANE) and cathepsin G (CTSG) mRNA levels in peripheral blood CD14(+) cells obtained from RA patients, healthy subjects (HS) and patients with ...
Wang Fang F Department of Biochemistry, Temple University School of Medicine, Philadelphia, - - 2014
Acinus-S' is a co-repressor for retinoic acid receptor (RAR)-dependent gene transcription and has been suggested to be involved in RNA processing. In this study the role of Acinus isoforms in regulating pre-mRNA splicing was explored using in vivo splicing assays. Both Acinus-L and Acinus-S', with the activity of Acinus-L higher ...
Sharma Sriganesh B SB Department of Biochemistry Program in Cancer Cell - - 2014
Despite the low prevalence of activating point mutation of RAS or RAF genes, the RAS-ERK pathway is implicated in breast cancer pathogenesis. Indeed, in triple-negative breast cancer (TNBC) there is recurrent genetic alteration of pathway components. Using shRNA methods, we observed that the zinc finger transcription factor Kruppel-like factor 4 ...
Ji Jianjian J The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing 210093, - - 2014
Increased IL-17-producing helper T (Th17) cells have been observed in patients with rheumatoid arthritis (RA). The retinoic-acid-related orphan nuclear receptor (RORγt) is the master regulator of Th17 cells. Our previous research showed that FC99 possesses anti-inflammation activity. However, to date the effects of FC99 on RORγt expression in Th17 cell ...
Miao Cheng-Gui CG School of food and drug, Anhui key laboratory of poultry epidemic prevention and surveillance, Anhui Science and Technology University, Bengbu 233100, China; School of pharmacy, Anhui key laboratory of bioactivity of natural products, Anhui Medical University, Hefei 230032, - - 2014
Rheumatoid arthritis (RA) is an autoimmune and progressive systemic disease of unknown etiology. Research shows that fibroblast-like synoviocytes (FLS) participate in the cartilage erosion, synovial hyperplasia, inflammatory cytokine secretion and suggests that fibroblast-like synoviocytes (FLS) display a crucial role in RA pathogenesis. Recent studies have suggested the role of the ...
Sfikakis P P PP Rheumatology Unit, First Department of Propaedeutic and Internal Medicine, Athens University Medical School, Greece. Electronic address: - - 2014
Rheumatoid arthritis (RA) synovial fibroblasts hyperexpress the mesenchymal cadherin-11, which is involved also in tumor invasion/metastasis, whereas anti-cadherin-11 therapeutics prevent and reduce experimental arthritis. To test the hypothesis that cadherin-11 is aberrantly expressed in RA peripheral blood, 100 patients (15 studied serially) and 70 healthy controls were analyzed by real-time ...
Cheong Kyung Ah KA Department of Dermatology, Dongguk University Ilsan Hospital, Graduate School of Medicine, 814 Siksa-dong, Ilsandong-gu, Goyang-si, Gyeonggi-do 410-773, South - - 2014
Hydroquinone (HQ) with or without retinoic acid (RA) is routinely used for the treatment of hyperpigmented conditions. Skin irritation is a major problem with popular depigmenting agents, resulting in postinflammatory hyperpigmentation. To examine the molecular mechanism associated with skin irritation by RA or HQ. A genome-wide transcriptional profiling analysis was ...
van Baarsen Lisa - - 2014
IntroductionAccumulating evidence suggests an important role for interleukin (IL)-17 in the pathogenesis of several inflammatory diseases, including rheumatoid arthritis (RA) and psoriatic arthritis (PsA). Accordingly, clinical trials aimed at blocking IL-17 have been initiated but clinical results are highly variable between patients and across different diseases. The objective was to ...
Biniecka Monika M Translation Research Group, Dublin Academic Medical Centre, St. Vincent's University Hospital, Dublin, - - 2014
Objective: Inflammatory arthritis (IA) is associated with joint inflammation, synovial tissue proliferation and degradation of articular cartilage and bone. Angiogenesis is an early and fundamental component of synovial inflammation. Oxygen metabolism is recognized as an important mediator of joint vascular remodeling. In this study we investigate if in-vivo synovial hypoxia ...
Lee Hye-Kyung HK Seoul National University, Korea, Republic - - 2014
Odontoblasts are a type of terminally differentiated matrix-secreting cells. A number of molecular mechanisms are involved in the differentiation of odontoblasts. Several studies demonstrated that Kruppel-like factor 4 (KLF4) promotes odontoblast differentiation via control of dentin sialophosphoprotein (DSPP). Because nuclear factor I-C (NFIC) is also known to control DSPP, we ...
Kero D D School of Dental Medicine, University of Split, Soltanska 2, 21000, Split, - - 2014
Spatio-temporal immunolocalizations of cytokeratin 8 (CK8), vimentin, syndecan-1 and Ki-67 were analyzed in ten human incisors and canine tooth germs between the 7th and 20th developmental weeks. CK8 expression was mild to moderate in the epithelial tooth parts, while it shifted from absent or mild in its mesenchymal parts, but ...
Kuzynski Maria M University of Alabama at Birmingham, United - - 2014
TRPS1 is a unique GATA-type transcription factor that acts as a transcriptional repressor. TRPS1 deficiency (tricho-rhino-phalangeal syndrome, TRPS) and dysregulated TRPS1 expression (Ambras syndrome) result in skeletal and dental abnormalities implicating TRPS1 in endochondral bone formation and tooth development. Moreover, TRPS patients frequently present with low bone mass indicating TRPS1 ...
Jeong Youngdan Y Department of Conservative Dentistry, Ulsan University Asan Medical Center, Seoul, - - 2014
The effects of bone morphogenetic protein-2 (BMP-2) and enamel matrix derivative (EMD) respectively with mineral trioxide aggregate (MTA) on hard tissue regeneration have been investigated in previous studies. This study aimed to compare the osteogenic effects of MTA/BMP-2 and MTA/EMD treatment in MC3T3-E1 cells. MC3T3-E1 cells were treated with MTA ...
Lin Shuxian S Baylor College of Dentistry, Txas A&M University; - - 2014
Dentin Matrix Protein 1 (DMP1) plays multiple roles in bone, tooth, phosphate homeostasis, kidney, salivary gland, reproductive cycles, and the development of cancer. In vitro studies have indicated two different biological mechanisms: 1) As a matrix protein, DMP1 interacts with αvβ3 integrin and activates MAP kinase signaling; and 2) DMP1 ...
Wu Y Y The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei, 430079, - - 2014
Human dental pulp cells (HDPCs) with multi-potential differentiational capacity can undergo odontoblastic differentiation when stimulated with proinflammatory cytokines. However, factors linking proinflammatory stimuli and their odontoblastic differentiation have, as yet, not been completely understood. As an apoptotic transcription factor, DDIT3 plays a crucial role in the inflammatory reaction and in ...
Kim E J EJ Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, - - 2014
MicroRNAs (miRNAs) are known to regulate a variety of gene functions in many tissues and organs, but their expression and function in tooth development are not well-understood. A specific miRNA, miR-135a, was determined to be highly expressed at the bud stage. Interestingly, after the cap stage, miR-135a was expressed in ...
Kawasaki Maiko M Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Guy's Hospital, London Bridge, London, UK; Division of Bio-Prosthodontics, Department of Oral Health Science, Course for Oral Life Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, - - 2014
Background: Tooth development is highly regulated in mammals and it is regulated by networks of signaling pathways (e. g. Tnf, Wnt, Shh, Fgf and Bmp) whose activities are controlled by the balance between ligands, activators, inhibitors and receptors. The members of the R-spondin family are known as activators of Wnt ...
Liu M M Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA 02115, - - 2014
Teeth develop through distinct morphological stages. At the cap stage, a compactly clustered and concentrically arranged cell mass, the enamel knot, appears at the tip of the enamel organ. Cells in this knot express sets of key molecules, and as such have been proposed to act as a signaling center ...
Kim J W JW Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, - - 2014
ATF6 is an endoplasmic reticulum (ER) membrane-bound transcription factor that regulates various cellular functions. The purpose of this study was to investigate the role of ATF6 in odontoblast differentiation. Rat tooth germs were isolated, changes in gene expression were evaluated over time, and localization of ATF6 was determined by immunohistochemistry. ...
Yu Yan Y Institute of Stomatology, Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu, 210029, - - 2014
Dentin, the predominant mineralized tissue of the tooth, comprises an extracellular matrix of collagen and a heterogeneous mixture of non-collagenous components, many of which have cellular signaling properties. These properties may be important in signaling stem cell involvement in tissue regeneration following injury and the present study investigates their morphogenic ...
Shin Masashi M Department of Mineralized Tissue Biology and Harvard School of Dental Medicine, The Forsyth Institute, Cambridge Massachusetts, United States of - - 2014
Matrix metalloproteinase-20 (Mmp20) ablated mice have enamel that is thin and soft with an abnormal rod pattern that abrades from the underlying dentin. We asked if introduction of transgenes expressing Mmp20 would revert this Mmp20 null phenotype back to normal. Unexpectedly, for transgenes expressing medium or high levels of Mmp20, ...
Han Nana N State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Lab of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei, - - 2014
An intense stimulus can cause death of odontoblasts and initiate odontoblastic differentiation of stem/progenitor cell populations of dental pulp cells (DPCs), which is followed by reparative dentin formation. However, the mechanism of odontoblastic differentiation during reparative dentin formation remains unclear. This study was to determine the role of β-catenin, a ...
Reibring Claes-Göran CG Department of Oral Biochemistry, Sahlgrenska Academy at the University of Gothenburg, Göteborg, - - 2014
Carbonic anhydrases (CAs) play fundamental roles in several physiological events, and emerging evidence points at their involvement in an array of disorders, including cancer. The expression of CAs in the different cells of teeth is unknown, let alone their expression patterns during odontogenesis. As a first step towards understanding the ...
Wang Tong T Guanghua School of Stomatology & Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China; Hefei Stomatological Hospital, Hefei, - - 2014
p300 is a well-known histone acetyltransferase (HAT) and coactivator that plays vital roles in many physiological processes. Despite extensive research on the involvement of p300 in the regulation of transcription in numerous cell lines, the roles of this protein in regulating pluripotency genes and odontogenic differentiation in human dental pulp ...
Swales Catherine C Botnar Research Centre, Nuffield Department of Orthopaedics Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, United - - 2014
Osteoclasts are responsible for the bone loss associated with rheumatoid arthritis (RA). The secreted adipokine angiopoietin-like 4 (ANGPTL4) specifically increases osteoclast-mediated bone resorption. We have investigated expression of ANGPTL4 and its regulatory transcription factor, hypoxia-inducible factor-1 alpha (HIF-1α), in osteoclasts and other cells within rheumatoid synovium. We have also examined ...
Yang Wei-Hung WH Department of Orthopedic Surgery, Taichung Hospital, Department of Health Executive Yuan, Taichung 403, Taiwan. - - 2014
Inflammatory response and articular destruction are common symptoms of osteoarthritis (OA) and rheumatoid arthritis (RA). Leptin, an adipocyte-secreted hormone that centrally regulates weight control, may exert proinflammatory effects in the joint, depending on the immune response. Yet, the mechanism of leptin interacting with the arthritic inflammatory response is unclear. This ...
Baba Otto O Section of Biology, Department of Oral Function and Molecular Biology, School of Dentistry, Ohu University, 31-1 Misumido, Tomita, Koriyama, Fukushima, 963-8611, Japan, - - 2013
Fibroblast growth factors (FGFs) regulate the proliferation and differentiation of various cells via their respective receptors (FGFRs). During the early stages of tooth development in fetal mice, FGFs and FGFRs have been shown to be expressed in dental epithelia and mesenchymal cells at the initial stages of odontogenesis and to ...
Pääkkönen V V Institute of Dentistry, University of Oulu, - - 2013
To study the expression of Toll-like receptors (TLR) -3, -7, -8 and -9 as well as interferon receptors alpha and gamma (IFNAR1/IFNAR2 and IFNGR1/IFNGR2), which play important roles in the defense against viruses. DNA microarray and quantitative PCR analyses of TLR3, -7, -8 and -9 as well as IFNAR1/IFNAR2 and ...
Lan Yu Y Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA; Division of Plastic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, - - 2013
Four conserved signaling pathways, including the bone morphogenetic proteins (Bmp), fibroblast growth factors (Fgf), Sonic hedgehog (Shh), and Wingless-related (Wnt) pathways, are each repeatedly used throughout tooth development. Inactivation of any of these resulted in early tooth developmental arrest in mice. The mutations identified thus far in human patients with ...
Sawada Takashi T Department of Ultrastructural Science, Tokyo Dental College, Misaki-cho 2-9-18, Chiyoda-ku, Tokyo, 101-0061, Japan, - - 2013
The biochemical composition of the internal and external basal laminae in the junctional epithelium differs significantly, and the precise cellular origin of their respective molecules remains to be determined. In the present study, the expression and localization of three basement membrane-specific molecules-laminin 5 (γ2 chain), type IV collagen (α1 chain), ...
Nociti F H FH Jr National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, - - 2013
Calcium and phosphorus homeostasis is achieved by interplay among hormones, including 1,25(OH)2D3 (1,25D), parathyroid hormone, and fibroblast growth factor 23 (FGF23), and their interactions with other proteins. For example, mutations in dentin matrix protein 1 (DMP-1) result in increased FGF23 and hypophosphatemic rickets. 1,25D is reported to modulate FGF23; thus, ...
Wen Xin - - 2013
The sodium pump Na(+) /K(+) -ATPase, expressed in virtually all cells of higher organisms, is involved in establishing a resting membrane potential and in creating a sodium gradient to facilitate a number of membrane-associated transport activities. Na(+) /K(+) -ATPase is an oligomer of α, β, and γ subunits. Four unique ...
Zeng Qi-Bing QB School of Public Health, Guiyang Medical University, Guiyang 550004, PR - - 2013
Chronic exposure to combined fluoride and arsenic continues to be a major public health problem worldwide, affecting thousands of people. In recent years, more and more researchers began to focus on the interaction between the fluorine and the arsenic. In this study, the selected investigation site was located in China. ...
Yuanyuan Wang - - 2013
The enamel matrix derivative (EMD) has a positive effect on the proliferation of human periodontal ligament cells and the healing of periodontal tissues. The aim of this study was to evaluate the effects of EMD on the proliferation and differentiation of human dental pulp cells (hDPCs) in vitro. hDPCs were ...
Chang Seok-Woo SW Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of - - 2014
The aim of this study was to compare the biocompatibility and odontogenic potential of newly developed Bioaggregate (BA) and Micromega MTA (MMTA) with ProRoot MTA (PMTA) and intermediate restorative material (IRM) by using human dental pulp cells. Biocompatibility was assessed by an 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium bromide assay and scanning electron microscopy. Differentiation ...
Yoo H I - - 2013
Odontogenesis, tooth development, is derived from two tissue components: ectoderm and neural crest-derived mesenchyme. Cyto-differentiation of odontogenic cells during development involves time-dependent and sequential regulation of genetic programs. This study was conducted to detect molecules implicated in cyto-differentiation of developing molar germs of rats. Differential display-PCR revealed that PrP(c) was ...
Guan X X Department of Mineralized Tissue Biology and Harvard School of Dental Medicine, The Forsyth Institute, 245 First Street, Cambridge, MA 02142, - - 2013
Matrix metalloproteinase-20 (enamelysin, MMP20) is essential for dental enamel development. Seven different MMP20 mutations in humans cause non-syndromic enamel malformations, termed amelogenesis imperfecta, and ablation of Mmp20 in mice results in thin brittle enamel with a dysplastic rod pattern. Healthy enamel formation requires the sliding movement of ameloblasts in rows ...
Koizumi Yu - - 2013
Congenital anomalies of wingless-type mouse mammary tumor virus (MMTV) integration site family (Wnt) are frequently accompanied with tooth and dentin abnormality. The aim of this study was to investigate the effects of Wnt signaling on odontoblast differentiation of mouse dental papilla cells (MDPs). Mouse dental papilla cells were cultured in ...
Li Xiao - - 2013
Patients with Axenfeld-Rieger Syndrome (ARS) present various dental abnormalities, including hypodontia, and enamel hypoplasia. ARS is genetically associated with mutations in the PITX2 gene, which encodes one of the earliest transcription factors to initiate tooth development. Thus, Pitx2 has long been considered as an upstream regulator of the transcriptional hierarchy ...
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