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Elkhatib Razan R Aix-Marseille Université-Inserm UMR 910, Génétique médicale et Génomique Fonctionnelle, 13385 Marseille cedex 5, - - 2014
The nuclear lamina (NL) is a filamentous protein meshwork, composed essentially of lamins, situated between the inner nuclear membrane and the chromatin. There is mounting evidence that the NL plays a role in spermatid differentiation during spermiogenesis. The mouse spermatid NL is composed of the ubiquitous lamin B1 and the ...
Melnikova Nataliya V NV Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 119991 Moscow, Russia. Electronic address: - - 2014
Effective fertilizer application is necessary to increase crop yields and reduce risk of plant overdosing. It is known that expression level of microRNAs (miRNAs) alters in plants under different nutrient concentrations in soil. The aim of our study was to identify and characterize miRNAs with expression alterations under excessive fertilizer ...
Ing N H NH Department of Animal Science, Texas A&M AgriLife Research, Texas A&M University, College Station, TX 77843-2471, United States. Electronic address: - - 2014
Testicular steroidogenesis and spermatogenesis are negatively impacted by stress-related hormones such as glucocorticoids. The effects of two injections of a therapeutic dose of dexamethasone (a synthetic glucocorticoid, 0.1mg/kg; i.v.) given 24h apart to each of three stallions were investigated and compared to three saline-injected control stallions. Dexamethasone decreased circulating concentrations ...
Warinrak C C Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, - - 2014
This study aims to investigate the role of matrix metalloproteinases (MMPs) in determining semen quality and to evaluate the expression and cellular localization of MMP-2, MMP-9, tissue inhibitor of metalloproteinase-1 (TIMP-1) and TIMP-2 in the testes, epididymis and ejaculated spermatozoa. Gelatinase activities between normal (n = 21) and abnormal (n = 25) semen samples ...
Yamamoto Y Y Okayama University, Okayama, - - 2014
Activin (ACV) is known as a local regulator of several reproductive functions including follicular development and implantation in mammals. ACVA is a glycopeptide belonging to the transforming growth factor β superfamily, and is a homodimer of inhibin ßA (INHBA) subunits. Follistatin (FST), an ACV-specific binding protein, inhibits ligand-receptor binding. ACV ...
Fontes P K PK Institute of Biosciences, UNESP, Univ Estadual Paulista, Botucatu, São Paulo, - - 2014
The oviduct plays a key role promoting a favourable microenvironment to gametes transport, fertilization and early embryo development. Numerous differences in reproductive physiology are known among animals of Zebu and European breeds. Reports indicate that female Zebu cattle have a higher number of follicles per wave than female European cattle ...
Uzbekova S S INRA UMR85 Physiologie de la Reproduction et des Comportements-CNRS UMR7247-Université François Rabelais de Tours-IFCE, Nouzilly, - - 2014
Oocyte maturation relies on energy from different nutrients, including fatty acids (FA). Cumulus cells (CC) are metabolically coupled with enclosed oocyte and active FA metabolism occurs in both compartments. Excess of lipids in oocyte environment alters its developmental competence. Lipid droplets (LD), mainly composed of triacylglycerides (TG), are formed inside ...
Uhde K K Department of Farm Animal Health, Utrecht University, Faculty of Veterinary Medicine, Utrecht, the - - 2014
A mammalian oocyte within an ovarian follicle is surrounded by cumulus cells, together this structure is known as the cumulus-oocyte complex (COC). Cumulus cells are important for the development of the oocyte, they support the maturation process of the oocyte within the ovary and aid in sperm recognition. Because it ...
Ikeda S S Kyoto University, Kyoto, Kyoto, - - 2014
Myo-inositol (MI) added into in vitro culture media stimulates blastocyst development of mammals, including cattle (Holm et al. 1999 Theriogenology 52, 683-700), and these stimulatory effects are considered to be exerted via the incorporation of MI into phosphatidylinositol (Hynes et al. 2000 Mol. Reprod. Dev. 55, 265-269). Myo-inositol is catabolized ...
Zhang Yu Y College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, - - 2014
Mammalian diaphanous1 (mDia1) is a homologue of Drosophila diaphanous and belongs to the Formin-homology family of proteins that catalyze actin nucleation and polymerization. Although Formin family proteins, such as Drosophila diaphanous, have been shown to be essential for cytokinesis, whether and how mDia1 functions during meiosis remain uncertain. In this ...
Figueira Marília I MI CICS-UBI - Health Sciences Research Centre, University of Beira Interior, Covilhã, - - 2014
The c-KIT, a tyrosine kinase receptor, and its ligand the stem cell factor (SCF) play an important role in the production of male and female gametes. The interaction of SCF with c-KIT is required for germ cell survival and growth, and abnormalities in the activity of the SCF/c-KIT system have ...
Zhang Yu Y a College of Animal Science and Technology , Nanjing Agricultural University , Nanjing , - - 2014
Mammalian oocyte maturation is distinguished by asymmetric division that is regulated primarily by cytoskeleton, including microtubules and microfilaments. Small Rho GTPase RhoA is a key regulator of cytoskeletal organization which regulates cell polarity, migration, and division. In this study, we investigated the roles of RhoA in mammalian oocyte meiosis and ...
Cullinane Danielle Louise DL D Cullinane, Department of Biology, University of Massachusetts Boston, Boston , United - - 2014
The protamine 1 (Prm1) and sperm mitochondria-associated cysteine-rich protein (Smcp) mRNAs exemplify a widespread pattern of mRNA-specific regulation of mRNA translation in post-meiotic spermatogenic cells, spermatids. Both mRNAs are transcribed and initially stored in free-mRNPs in early spermatids, and translated on polysomes in late spermatids. Here, we demonstrate that the ...
Kempisty Bartosz B Department of Histology and Embryology,Department of Anatomy,Poznan University of Medical Sciences,6 Święcickiego St.,60-781 - - 2014
Summary This study was aimed at investigating zona pellucida glycoproteins (ZP) ZP2, ZP3 mRNA expression as well as ZP3, ZP4 (ZPB) protein distribution before and after in vitro maturation (IVM) in canine oocytes. The cumulus-oocyte complexes (COCs) were recovered from 27 anoestrous mongrel bitches and matured for 72 h in ...
Pansa Alessandra A Medical Genetics, Department of Health Sciences, Università degli Studi di Milano, 20142 Milano, - - 2014
Is the presence of ESX1 mRNA in seminal fluid (SF) an indicator of residual spermatogenesis in men with non-obstructive azoospermic (NOA)? ESX1 mRNA in SF is a suitable molecular marker for predicting the presence of residual spermatogenesis in testis. ESX1 is an X-linked homeobox gene whose expression in testis is ...
Asokan Y Y Division of Clinical Embryology, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, - - 2014
To differentiate dead spermatozoa from viable but immotile spermatozoa, several techniques are being used during ICSI. As processed spermatozoa from poor-quality ejaculate are confronted with a higher risk of experiencing stress on exposure to altered osmotic conditions or chemicals, this study was undertaken to determine the expression of stress response ...
Tian Yonghong Y Reproductive Medicine Center, Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310006, - - 2014
Heat shock protein A2 (HspA2) expression was quantitatively measured in human testis and its relationship with the spermatogenetic status and laboratory outcomes of intracytoplasmic sperm injection (ICSI) was investigated. Testicular tissues of azoospermia men were divided into four groups according to histopahtology: normal spermatogenesiss, hypospermatogenesis, maturation arrest and Sertoli cell-only ...
Hoseini Fatemeh - - 2014
BackgroundThe main objective of the present work was to compare the effects of the gonadotropin-releasing hormone agonist (GnRH-a) and GnRH antagonist (GnRH-ant) on the gene expression profiles of oocytes obtained from Iranian infertile couples undergoing in vitro fertilization (IVF).MethodsFifty infertile couples who underwent IVF between June 2012 and November 2013 ...
Wang Jianbin J a Department of Biochemistry and Molecular Genetics , University of Colorado School of Medicine , Aurora , CO - - 2014
Abstract In mature gametes and during the oocyte-to-embryo transition, transcription is generally silenced and gene expression is post-transcriptionally regulated. However, we recently discovered that major transcription can occur immediately after fertilization, prior to pronuclear fusion, and in the first cell division of the oocyte-to-embryo transition in the nematode Ascaris suum. ...
Abhari Alireza A Department. of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, - - 2014
Background: MicroRNA (miRNA) is small endogenous, single strand RNA molecules that regulate gene expression at post-transcriptional level through several mechanisms to affect key cellular event including male germ cells differentiation, proliferation, development and apoptosis. Mutation and/or aberrant expression of miRNAs have been associated with progression of various disorders, including infertility. ...
Beymer Matthew M Department of Physiology and Biophysics, Stony Brook University Medical Center, Stony Brook, New York; Graduate Program in Genetics, Stony Brook University, Stony Brook, New - - 2014
Hypothalamic kisspeptin neurons integrate and translate cues from the internal and external environments that regulate gonadotropin-releasing hormone (GnRH) secretion and maintain fertility in mammals. However, the intracellular signaling pathways utilized to translate such information into changes in kisspeptin expression, release, and ultimately activation of the kisspeptin-receptive GnRH network have not ...
Liu Qian Q Center for Reproductive Medicine,Renmin Hospital of Wuhan University,238 Jie Fang - - 2014
Summary Osteopontin (OPN) is a multifunctional phosphoprotein that is detected in various tissues, including male and female reproductive tracts. In this study, we evaluated OPN expression in mouse oviducts during the estrus cycle, and at days 1-5 of pregnancy and pseudopregnancy by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. ...
Spike Caroline A CA Department of Genetics, Cell Biology and Development, University of Minnesota Minneapolis, Minnesota - - 2014
The oocytes of most sexually reproducing animals arrest in meiotic prophase I. Oocyte growth, which occurs during this period of arrest, enables oocytes to acquire the cytoplasmic components needed to produce healthy progeny and to gain competence to complete meiosis. In the nematode Caenorhabditis elegans, the major sperm protein hormone ...
Li Weina - - 2014
BackgroundHundreds of HSP40s derived from various species have been identified, of which several proteins are involved in spermatogenesis. DNAJB13 is a type II HSP40/ DnaJ protein. In a previous study, we cloned mouse Dnajb13, which is up-regulated in cryptorchidism. To date, however, little is known about the localization and functions ...
Santonocito Manuela M Department Gian Filippo Ingrassia, Biologia Cellulare e Molecolare, Genetica, Genomica Giovanni Sichel, Università di Catania, Catania, - - 2014
To characterize well-represented microRNAs in human follicular fluid (FF) and to ascertain whether they are cargo of FF exosomes and whether they are involved in the regulation of follicle maturation. FF exosomes were characterized by nanosight, flow cytometry, and exosome-specific surface markers. Expression microRNA profiles from total and exosomal FF ...
Yan Wei W Department of Physiology and Cell Biology, University of Nevada School of Medicine, 1664 North Virginia Street, MS575, Reno, NV 89557, USA. Electronic address: - - 2014
"Epigenetic Transgenerational Inheritance" (ETI) has been defined as germline (sperm or egg) transmission of epigenetic information between generations in the absence of direct exposures or genetic manipulations. Among reported cases of ETI in mammals, the majority are induced by environmental factors, including environmental toxicants [e.g. agricultural fungicide vinclozolin, plastic additive ...
Tscherner Allison - - 2014
Oocyte fertilization and successful embryo implantation are key events marking the onset of pregnancy. In sexually reproducing organisms, embryogenesis begins with the fusion of two haploid gametes, each of which has undergone progressive stages of maturation. In the final stages of oocyte maturation, minimal transcriptional activity is present and regulation ...
Mantripragada Venkata P VP Biomedical Engineering Program, The University of Toledo, Toledo, OH 43614-5807, - - 2014
The main objective of this study is to maximize growth factor encapsulation efficiency into microparticles. The novelty of this study is to maximize the encapsulated growth factors into microparticles by minimizing the use of organic solvents and using relatively low temperatures. The microparticles were fabricated using chitosan biopolymer as a ...
Bormann Sebastian S Energy Biosciences Institute, University of California, Berkeley, California 94720, - - 2014
Processes for the biotechnological production of kerosene and diesel blendstocks are often economically unattractive due to low yields and product titers. Recently, Clostridium acetobutylicum fermentation products acetone, butanol, and ethanol (ABE) were shown to serve as precursors for catalytic upgrading to higher chain-length molecules that can be used as fuel ...
Mou Chengzhi C Department of Neurosurgery, Shandong Provincial Hospital affiliated to Shandong University, 9677 Jingshi Road, Jinan, Shandong, 250021, - - 2014
Microglia can be driven to adopt M1 and M2 phenotypes. While the distinct functions of M1 and M2 microglia have been intensively studied, the former are considered proinflammatory and the latter anti-inflammatory. PGC-1-related coactivator (PRC), an important member in the peroxisome proliferator-activated receptor γ coactivator-1 (PGC-1) family, plays an essential ...
Emmons Scott W SW Albert Einstein College of Medicine, Bronx, NY, - - 2014
Studies of the development of the Caenorhabditis elegans male have been carried out with the aim of understanding the basis of sexual dimorphism. Postembryonic development of the two C. elegans sexes differs extensively. Development along either the hermaphrodite or male pathway is specified initially by the X to autosome ratio. ...
Zhou Hai H State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions; College of Life Sciences, South China Agricultural University, Guangzhou 510642, - - 2014
Thermosensitive genic male-sterile (TGMS) lines, which are male-sterile at restrictive (high) temperatures but male-fertile at permissive (low) temperatures, have been widely used in breeding two-line hybrid rice (Oryza sativa L.). Here we find that mutation of thermosensitive genic male sterile 5 (tms5) in rice causes the TGMS trait through a ...
Jing Jing J College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, Wuhan, - - 2014
Recently, YY super-male yellow catfish had been created by hormonal-induced sex reversal and sex-linked markers, which provides a promising research model for fish sex differentiation and gonad development, especially for testis development. MicroRNAs (miRNAs) have been revealed to play crucial roles in the gene regulation and gonad development in vertebrates. ...
Stowe Heather M HM Department of Animal and Veterinary Sciences, Clemson University, Clemson, South Carolina, United States of - - 2014
Tall fescue [Schedonorus phoenix (Scop.) Holub] accounts for nearly 16 million hectares of pasture in the Southeastern and Mid-Atlantic U.S. due to its heat, drought, and pest resistance, conferred to the plant by its symbiotic relationship with the endophyte Neotyphodium coenophialum. The endophyte produces ergot alkaloids that have negative effects ...
Jullien Frédéric - - 2013
In this paper we characterize three sTPSs: a germacrene D (LaGERDS), a (E)-β-caryophyllene (LaCARS) and a τ-cadinol synthase (LaCADS). τ-cadinol synthase is reported here for the first time and its activity was studied in several biological models including transiently or stably transformed tobacco species. Three dimensional structure models of LaCADS ...
Kim Haejin - - 2013
In the present study, methanolic extract of Ecklonia cava and its solvent-partitioned fractions were evaluated for their antiadipogenic effect in 3T3-L1 adipocytes. One of them, n-BuOH fraction, effectively reduced lipid accumulation and glucose consumption. In addition, the presence of n-BuOH fraction in adipocytes suppressed the regulations of adipogenic transcription factors, ...
Lecomte V - - 2013
Potamopyrgus antipodarum is a gastropod mollusk proposed for use in the development of reproduction tests within the Organization for Economic Cooperation and Development (OECD). Numerous chemicals, including endocrine disrupters, are relatively water-insoluble, and water-miscible solvents are currently used for testing them. OECD recommends a maximum concentration of 100μll(-1). As several ...
Kido Takamasa - - 2013
The use of a petroleum-derived cleaning solvent for dry cleaning, instead of tetrachloroethylene (perchloroethylene, PCE), has increased. The cleaning solvent may induce immunological alteration. In this study, murine macrophage-lineage J774.1 cells were exposed to the cleaning solvent at 0, 25, 50, and 75 µg/ml or PCE at 0, 400, 600, 800, ...
Szczyrba Jaroslaw - - 2012
In primary prostate cancer (PCa), a major cause of cancer-related death in men, the expression of various microRNAs (miRNAs) is deregulated. We previously detected several miRNAs, e.g., miR-24 and miR-22, as significantly down-regulated in PCa (1) . An in silico search predicted that zinc finger protein 217 (ZNF217) and importin ...
Tsai Shih-Jei - - 2012
Protocatechuic acid (PCA) at 0.5, 1 or 2% was supplied to d-galactose (DG) treated mice for 8 wks. PCA intake at 2% increased its deposit in brain. DG treatment increased brain level of reactive oxygen species, protein carbonyl, carboxymethyllysine, pentosidine, sorbitol, fructose and methylglyoxal (P<0.05). PCA intake, at 1 and ...
Fitting Sylvia - - 2012
Opiate abuse reportedly can exaggerate complications of human immunodeficiency virus type-1 (HIV-1) infection in the central nervous system (CNS), while opiate drugs are often indicated in the treatment of HIV-1-related neuropathic pain. Despite this quandary, few studies have assessed the relationship between the duration or extent of HIV-1 infection and ...
Imbrie Gregory A GA Hematology-Oncology Section, Department of Medicine, Boston University Medical School, 650 Albany Street, Boston, MA, - - 2012
The establishment of the dorso-ventral axis is a fundamental process that occurs after fertilization. Dorsal axis specification in frogs starts immediately after fertilization, and depends upon activation of Wnt/β-catenin signaling. The protein kinase CK2α can modulate Wnt/β-catenin signaling and is necessary for dorsal axis specification in Xenopus laevis. Our previous ...
Wu Qiang - - 2012
cAMP response element-binding protein(CREB) and the cAMP cascade play a pivotal role in the opiate-dependence. The blockade of this cascade is believed to attenuate signs of physical opiate withdrawal. A lentiviral vector (LV) expressing a small hairpin RNA (shRNA) to silence CREB in vitro and in vivo was used in ...
Vijayan Lakshmy - - 2012
L-type calcium channel blockers like verapamil are used in the prophylaxis of migraine. However, their effect on the expression of CGRP in the trigeminal nucleus caudalis (TNC) is unknown. It is important because an earlier study had shown that olcegepant, a CGRP receptor antagonist, acts at the level of the ...
Kao Sheng-Chin SC Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, - - 2012
Cumulating evidence has demonstrated that μ opioid receptor (MOR) agonists promote spinal glial activation, lead to synthesis and release of proinflammatory cytokines and chemokines, and contribute to opioid-induced hyperalgesia and development of opioid tolerance and dependence. However, whether these MOR agonists directly or indirectly act on spinal cord astrocytes and ...
Jalali Mashayekhi Farideh - - 2012
Brain-derived neurotrophic factor (BDNF) plays a role in mediating molecular, cellular, and behavioral adaptations underlying drug addiction. Here, we examined the influence of withdrawal from repeated morphine treatment on the expression of BDNF mRNA in the ventral tegmental area (VTA) and locus coeruleus (LC) of the rat brain. We also ...
Berta Temugin - - 2012
Activation of spinal cord glial cells such as microglia and astrocytes has been shown to regulate chronic opioid-induced antinociceptive tolerance and hyperalgesia, due to spinal up-regulation of the proinflammatory cytokines such as interleukin-1 beta (IL-1β). Matrix metalloprotease-9 (MMP-9) has been implicated in IL-1β activation in neuropathic pain. However, it is ...
He Ying - - 2012
This chapter will focus on the role of microRNAs (miRs) in regulating the actions of opioid drugs through the opioid receptors. Opioids, such as morphine, are analgesics that are used for treating many forms of acute and chronic pain. However, their chronic use is limited by undesirable effects such as ...
Chokechanachaisakul Uraiwan - - 2011
In normal dental pulp, a considerable number of resident macrophages are distributed. This study was designed to analyze the expression levels of genes associated with differentiation and function of resident macrophages in rat molar pulps stimulated with lipopolysaccharide (LPS). Mandibular first molars of 7-week-old male Wistar rats were used. After ...
Lorch Yahli Y Department of Structural Biology, Stanford University School of Medicine, Stanford, California, USA. - - 2011
Purified chromatin rings, excised from the PHO5 locus of Saccharomyces cerevisiae in transcriptionally repressed and activated states, were remodeled with RSC and ATP. Nucleosomes were translocated, and those originating on the promoter of repressed rings were removed, whereas those originating on the open reading frame (ORF) were retained. Treatment of ...
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