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Zhang Qin Q Qin Zhang, Chuan-Shan Zhang, Qi Xin, Zhe Ma, Gui-Qiu Liu, Bing-Bing Liu, Department of Pathology, the Third Central Hospital of Tianjin Medical University, Tianjin 300170, - - 2014
To investigate if loss of epithelial cell adhesion molecule (EpCAM) is associated with microinvasion in hepatocellular carcinomas (HCCs) in the presence of chronic hepatitis B. The expression of EpCAM, cytokeratin 7 (CK7) and CK19 in 112 hepatic nodules was studied, including 20 HCCs with nodules ≤ 3 cm, 26 HCCs ...
Krishna Chadalavada Vijay CV Thomas Jefferson - - 2014
Background & Aims: A compromise in the internal anal sphincter (IAS) tone and fibroelastic properties (FEP) plays an important role in rectoanal incontinence (RI). Herein, we examined the effects of (HO-1) upregulation on these IAS characteristics in young rats. Methods: We determined the effect of HO-1 upregulator hemin on HO-1 ...
Wan Pin-Jun PJ Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, - - 2014
Postembryonic development of insects is highly dependent on ecdysteroid hormones ecdysone (E) and 20-hydroxyecdysone (20E). A cytochrome P450 monooxygenase CYP306A1, the product of the Halloween gene phantom (phm), is involved in the ecdysteroidogenesis in representative insects in Diptera, Lepidoptera and Orthoptera. In the present paper, Sfphm was cloned from a ...
Utashima Yuu Y Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashihiroshima, Hiroshima, 739-8528, - - 2014
In the present study, we attempted to improve the production of recombinant horseradish peroxidase C1a (HRP-C1a; a heme-binding protein) by Cryptococcus sp. S-2. Both native and codon-optimized HRP-C1a genes were expressed under the control of a high-level expression promoter. When the HRP-C1a gene with native codons was expressed, poly(A) tails ...
Xu Jinjin J 1Department of Anesthesiology, The University of Hong Kong, Hong Kong SAR, China. 2Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China. 3Department of Anesthesiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China. 4Department of Pharmacology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada. 5Department of Anesthesiology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, - - 2014
Heme oxygenase-1 is inducible in cardiomyocytes in response to stimuli such as oxidative stress and plays critical roles in combating cardiac hypertrophy and injury. Signal transducer and activator of transcription 3 plays a pivotal role in heme oxygenase-1-mediated protection against liver and lung injuries under oxidative stress. We hypothesized that ...
Chen Jane-Jane JJ Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, - - 2014
This review will provide an overview of the translational regulation of globin mRNAs and integrated stress response (ISR) during erythropoiesis by heme-regulated eIF2α kinase (HRI). HRI is an intracellular heme sensor that coordinates heme and globin synthesis in erythropoiesis by inhibiting protein synthesis of globins and heme biosynthetic enzymes during ...
Tanioka Nohito N Department of Anesthesiology and Resuscitology, Okayama University Medical School, Okayama 700-8558, - - 2014
Hepatic oxidative stress is a major contributor to the pathogenesis of several acute liver diseases. Diagnostic markers of hepatic oxidative stress may facilitate early detection and intervention. Bach1 is an oxidative stress-responsive transcription factor that represses heme oxygenase 1 (HO-1), the rate-limiting enzyme in the catabolism of heme, a potent ...
Chen Ren-Jin RJ Laboratory Animal Center, Xuzhou Medical College, Xuzhou, 221004, Jiangsu, - - 2014
Heme oxygenase (HO) represents an intrinsic antiinflammatory system based on its ability to inhibit expression of proinflammatory cytokines. The constitutive isoform heme oxygenase-2 (HO-2) has high expression and activity in cerebral microvascular endothelial cells (CMVEC). This study was undertaken to evaluate the role of HO-2 in regulation of TLR4/MyD88-dependent signaling ...
Cuadrado Antonio A CSIC-UAM,, - - 2014
The small GTPase protein RAC1 participates in innate immunity by activating a complex program that includes cytoskeleton remodeling, chemiotaxis, activation of NADPH oxidase and modulation of gene expression. However, its role in regulating the transcriptional signatures that in term control the cellular inflammatory profiles are not well defined. Here, we ...
Michaeloudes Charalambos C Airway Disease Section, National Heart and Lung Institute, Imperial College London and Biomedical Research Unit, Royal Brompton Hospital, London SW3 6LY, United - - 2014
Oxidative stress, a pathogenetic factor in many conditions, including chronic obstructive pulmonary disease, arises due to accumulation of reactive oxygen species and defective antioxidant defenses in the lungs. The latter is due, at least in part, to impaired activation of NF-E2-related factor 2 (Nrf2), a transcription factor involved in the ...
Wang Zhe Z Department of Pediatrics, University of Mississippi Medical Center, Jackson, Mississippi, - - 2014
Rhabdomyolysis accounts for ∼10% of acute kidney injuries. In glycerol-induced myoglobinuric acute kidney injury, we found an increase in the nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear protein, a key redox-sensitive transcription factor, and Nrf2-regulated genes and proteins including upregulation of heme oxygenase-1. In in vitro studies, pretreatment of ...
Zhang Shuqing S Soochow University, Suzhou 215123, Jiangsu, - - 2014
AbstractUsing a zebrafish model for hepatoerythropoietic porphyria (HEP), we found a new mechanism underlying heme regulation of exocrine zymogens. Zebrafish bach1b, nrf2a and mafK are all expressed in the zebrafish exocrine pancreas. Overexpression of bach1b and knockdown of nrf2a result in downregulation of the zymogens, whereas overexpression of nrf2a and ...
Zhou Chenghui C 1Department of Anesthesiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, - - 2014
Although both sevoflurane postconditioning (SPoC) and delayed remote ischemic preconditioning (DRIPC) have been proved effective in various animal and human studies, the combined effect of these 2 strategies remains unclear. Therefore, this study was designed to investigate this effect and elucidate the related signal mechanisms in a Langendorff perfused rat ...
Haldar Malay M Department of Pathology and Immunology, Washington University in St. Louis, School of Medicine, St. Louis, MO 63110, - - 2014
Splenic red pulp macrophages (RPM) degrade senescent erythrocytes and recycle heme-associated iron. The transcription factor SPI-C is selectively expressed by RPM and is required for their development, but the physiologic stimulus inducing Spic is unknown. Here, we report that Spic also regulated the development of F4/80(+)VCAM1(+) bone marrow macrophages (BMM) ...
Ryu Min Ju MJ Food and Nutrition, Duksung Women's University, Seoul, 132-714, Republic of - - 2014
The cytoprotective mechanism of 7, 8-dihydroxyflavone (DHF) against oxidative stress-induced cell damage with respect to its stimulatory effect on the expression of heme oxygenase-1 (HO-1), a potent antioxidant enzyme, was investigated in the present study. Up-regulation of HO-1 expression by DHF was both dose and time dependent in lung fibroblast ...
Hada Hiroshi H Department of Biochemistry, and Center for Regulatory Epigenome and Disease, Tohoku University Graduated School of Medicine, Sendai 980-8575, Japan; CREST, Japan Science and Technology Agency, Sendai 980-8575, - - 2014
Intracellular heme plays versatile roles in a variety of physiological processes including mitochondrial respiration. Heme also induces the expression of genes such as heme oxygenase-1 (HO-1) by inactivating the transcription repressor Bach1 through direct binding. However, the source of heme for the regulation of the Bach1-HO-1 axis has been unclear. ...
Luo Yun-Peng YP Department of ICU, The First Affiliated Hospital of Harbin Medical University, Harbin, - - 2014
NLRP3 inflammasome activation contributes to acute lung injury (ALI), accelerating caspase-1 maturation, and resulting in IL-1β and IL-18 over-production. Heme oxygenase-1 (HO-1) plays a protective role in ALI. This study investigated the effect of hemin (a potent HO-1 inducer) on NLRP3 inflammasome in sepsis-induced ALI. The sepsis model of cecal ...
Warrington Junie Paula JP University of Mississippi Medical - - 2014
Preeclampsia is thought to arise from inadequate cytotrophoblast migration and invasion of the maternal spiral arteries, resulting in placental ischemia and hypertension. Evidence suggests that altered expression of Epithelial Na(+) Channel (ENaC) proteins may be a contributing mechanism for impaired cytotrophoblast migration. ENaC activity is required for normal cytotrophoblast migration. ...
Nishio Yoshiaki Y Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan; Department of Biomedical Sciences, Tokyo Denki University, Saitama, - - 2014
5-Aminolevulinic acid (5-ALA) is the naturally occurring metabolic precursor of heme. Heme negatively regulates the Maf recognition element (MARE) binding- and repressing-activity of the Bach1 transcription factor through its direct binding to Bach1. Heme oxygenase (HO)-1 is an inducible enzyme that catalyzes the rate-limiting step in the oxidative degradation of ...
Lee Dong-Sung DS Hanbang Body Fluid Research Center, Wonkwang University, Iksan, 570-749, - - 2014
In Korea and China, the heartwood of Dalbergia odorifera T. Chen is an important traditional medicine used to treat blood disorders, ischemia, swelling, and epigastric pain. In this study, we investigated the inhibitory effects of latifolin, a major neoflavonoid component isolated from the MeOH extract of D. odorifera, on the inflammatory ...
Laurindo Francisco R M FR Heart Institute (InCor), University of São Paulo School of Medicine , Vascular Biology Laboratory , Av Eneas C Aguiar, 44 - Annex II, 9th floor, São Paulo, São Paulo, Brazil, 05403-000, +55(11) 3069 5968 ; - - 2014
Significance- Understanding isoform- and context-specific subcellular Nox NADPH oxidase compartmentalization allows relevant functional inferences. This review addresses the interplay between Nox NADPH oxidases and the endoplasmic reticulum (ER), an increasingly evident player in redox pathophysiology given its role in redox protein folding and stress responses. Recent advances-Catalytic/regulatory transmembrane subunits are ...
Dennery Phyllis A PA University of Pennsylvania School of Medicine, 3401 Civic Center Blvd, Division of Neonatology, Philadelphiia, Pennsylvania, United States, 19072 ; - - 2014
Significance: Premature and sick neonates are often exposed to high concentrations of oxygen, which results in lung injury and long term adverse consequences. Nevertheless, neonates are more tolerant to hyperoxia than adults. This may be in part explained by high lung content of heme oxygenase (HO-1), the rate-limiting enzyme in ...
Klickovic Uros U Department of Clinical Pharmacology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, - - 2014
Purpose. Heme oxygenase-1 (HO-1) has been proposed to exert pharmacological benefits by its antioxidative and anti-inflammatory effects. HO-1 expression may be affected by the GT length polymorphism in the promoter region of the HO-1 gene. We investigated the inducibility of HO-1 by orally administered curcumin in healthy male subjects and ...
Joo Choi Ran R Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-742, - - 2014
Heme oxygenase-1 (HO-1) is an important anti-inflammatory, antioxidative and cytoprotective enzyme that is regulated by the activation of the major transcription factor, nuclear factor (erythroid-derived 2)-like 2 (Nrf2). In the present study, six stilbene derivatives isolated from Rheum undulatum L. were assessed for their antioxidative potential. In the tert-butylhydroperoxide (t-BHP)-induced ...
Zenclussen Maria Laura ML Department of Experimental Obstetrics and Gynecology, Medical Faculty, Otto-von-Guericke University , Magdeburg , - - 2014
Deletion of the heme oxygenase-1 (HO-1) (Hmox1) locus in mice results in intrauterine lethality. The expression of the heme catabolizing enzyme encoded by this gene, namely HO-1, is required to successfully support reproductive events. We have previously observed that HO-1 acts at several key events in reproduction ensuring pregnancy. HO-1 ...
You Xiaoxing X Institution of Pathogenic Biology, Medical College, University of South China, Hengyang City, P.R. - - 2014
This study is to investigate the mechanisms by which macrophage-activating lipopeptide-2 (MALP-2) induces heme oxygenase (HO)-1, a cytoprotective enzyme that catalyzes the degradation of heme, in human monocytes. Human monocytic THP-1 cells were cultured for transient transfection with plasmids and stimulation with MALP-2 for indicative time intervals. After incubation with ...
Sugiyama Yoshinori Y Innovative Beauty Science Laboratory, Kanebo Cosmetics Inc., Odawara, Japan ; Department of Biomedical Engineering, Toin University of Yokohama, Yokohama, - - 2014
Aquaporin 9 (AQP9) is a member of the aquaglyceroporin family that transports glycerol, urea and other small solutes as well as water. Compared to the expression and function in epidermal keratinocytes of AQP3, another aquaglyceroporin, our knowledge of epidermal AQP9 remains elusive. In this study, we investigated the expression of ...
Sun J J Dept. of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, PO Box 33932, Shreveport, LA 71130, USA; Dept. of Obstetrics and Gynecology, First Hospital, Harbin Medical University, Harbin, - - 2013
In this study, we determined if vitamin D could inhibit oxidative stress-induced thromboxane production by placental trophoblasts. Trophoblast isolated from normal placentas were stimulated with CoCl2, a hypoxic mimicking agent, with or without pretreatment of 1,25(OH)2D3. Soluble phospholipase-A2, metabolites of thromboxane-A2 and prostacyclin, and 8-isoprostane were measured. Expression of cyclooxygenase-1 ...
Xin Gang G Department of Obstetrics and Gynecology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. - - 2014
The anti-oxidative stress effect of heme oxygenase-1 (HO-1) expression is being increasingly studied. However, few studies regarding HO-1 have been conducted in patients with gestational diabetes mellitus (GDM). In the present study, HO-1 expression was compared in peripheral blood mononuclear cells at 24-28 weeks of pregnancy in patients with GDM ...
Lastres-Becker Isabel - - 2013
The chemokine fractalkine modulates microglial responses in neurodegenerative diseases, including tauopathies, but the mechanistic processes and their relevance in human brain pathologies is not yet known. Here, we show that hippocampal HT22 cells expressing human TAU(P301L) mutant protein produce fractalkine, which in microglia activates AKT, inhibits glycogen synthase kinase-3β and ...
Liu Lifang - - 2013
Due to limitations associated with whole blood for transfusions (antigen compatibility, transmission of infections, supply and storage), the use of cell-free hemoglobin as an oxygen carrier substitute has been in the center of research interest for decades. Human hemoglobin has previously been synthesized in yeast, however the challenge is to ...
Lee Jae-Hyoung - - 2013
Prostaglandin (PG) E2 is an immunomodulatory lipid mediator generated mainly via the cyclooxygenase-2 (COX-2) pathway from arachidonic acid at sites of infection and inflammation. A positive feedback loop of PGE2 on COX-2 expression is critical for homeostasis during toll-like receptor (TLR)-mediated inflammatory processes. The mechanism of PGE2-regulated COX-2 expression remains ...
Linares María - - 2013
Oxidative stress has been attributed both a key pathogenic and rescuing role in cerebral malaria (CM). In a Plasmodium berghei ANKA murine model of CM, host redox signaling and functioning were examined during the course of neurological damage. Host antioxidant defenses were early altered at the transcriptional level indicated by ...
Butler Geraldine - - 2013
The response of eukaryotic microbes to low-oxygen (hypoxic) conditions is strongly regulated at the level of transcription. Comparative analysis shows that some of the transcriptional regulators (such as the sterol regulatory element-binding proteins, or SREBPs) are of ancient origin and probably regulate sterol synthesis in most eukaryotic microbes. However, in ...
Kang Kai - - 2013
This study investigated the effects of heme oxygenase (HO)-1 on thrombomodulin (TM) and endothelial protein C receptor (EPCR) expression in sepsis-induced kidney injury. The role of HO-1 was evaluated in a cecal ligation and puncture (CLP)-induced model. Wistar rats were randomly assigned into 4 groups: sham, CLP, CLP + hemin (an HO-1 ...
Kanzaki Hiroyuki H Maxillo-Oral Disorders, Department of Oral and Maxillofacial Surgery, Tohoku University Hospital, Sendai 980-8575, Japan. - - 2013
Reactive oxygen species (ROS) act as intracellular signaling molecules in the regulation of receptor activator of nuclear factor-κB ligand (RANKL)-dependent osteoclast differentiation, but they also have cytotoxic effects that include peroxidation of lipids and oxidative damage to proteins and DNA. Cellular protective mechanisms against oxidative stress include transcriptional control of ...
Wang Zhepeng - - 2013
Blue egg coloring is attributed to biliverdin derived from the oxidative degradation of heme through catalysis by heme oxygenase (HO). The pigment is secreted into the eggshell by the shell gland. There is uncertainty as to whether the pigment is synthesized in the shell gland or in other tissues. To ...
Okita Yukari - - 2013
Transforming growth factor-β (TGF-β) has multiple functions in embryogenesis, adult homeostasis, tissue repair, and development of cancer. Here, we report that TGF-β suppresses the transcriptional activation of the heme oxygenase-1 gene (HO-1), which is implicated in protection against oxidative injury and lung carcinogenesis. HO-1 is a target of the oxidative ...
Itoh Ryuhei - - 2013
Several factors involved in the core circadian rhythm are PAS domain proteins, one of which, neuronal PAS2 (NPAS2), contains a heme-binding motif. It is thought that heme controls the transcriptional activity of core circadian factors BMAL1-NPAS2, and that the heme-binding nuclear receptor REV-erbα negatively regulates the expression of BMAL1. To ...
Kang Chang-Hee - - 2013
In this study, we found that 5-hydroxy-3,6,7,8,3'4'-hexamethoxyflavone (5HHMF) from Hizikia fusiforme considerably inhibits lipopolysaccharide (LPS)-stimulated NO production by suppressing the expression of inducible NO synthase (iNOS) in BV2 microglia. In addition, 5HHMF blocked LPS-induced phosphorylation of IκB, resulting in suppression of the nuclear translocation of nuclear factor-κB (NF-κB) subunits, namely ...
Bonkovsky Herbert L HL Liver, Digestive and Metabolic Disorders Laboratory, Cannon Research Center, Carolinas Medical Center, 1542 Garden Terrace, Charlotte, NC 28203, USA. - - 2013
To assess effects of heme on messenger RNA (mRNA) and microRNA (miRNA) profiles of liver cells derived from humans. We exposed human hepatoma cell line Huh-7 cells to excess iron protoporphyrin (heme) (10 μmol/L) or induced heme deficiency by addition of 4, 6-dioxoheptanoic acid (500 μmol/L), a potent inhibitor of ...
Vanella Luca - - 2013
INTRODUCTION: Heme oxygenase (HO), a major cytoprotective enzyme, attenuates oxidative stress and obesity. The canonical Wnt signaling cascade plays a pivotal role in the regulation of adipogenesis. The present study examined the interplay between HO-1and the Wnt canonical pathway in the modulation of adipogenesis in mesenchymal stem cell (MSC) derived ...
Lu Hui-Yuan - - 2013
Human cytochrome P450 2A13 (CYP2A13), mainly expressed in respiratory tract, is active toward numerous toxicants. To establish the metabolism in vitro, we expressed CYP2A13 and NADPH-CYP450 oxidoreductase (POR) in a baculovirus/sf9 system. Due to the deficiency of sf9 cells in heme incorporation, we investigated the effects of different heme precursors ...
Shiota Mitsuru - - 2013
Before establishment of feto-placental circulation, decidua can synthesize hemoproteins to maintain oxygen homeostasis in situ. Using the human decidua of induced abortions ranging from 5 to 8 weeks of gestation, we determined the expression levels of erythropoietin, erythropoietin receptor, cytoglobin, myoglobin, embryonic-, fetal- and adult hemoglobin mRNA by quantitative RT-PCR ...
Jin J Y - - 2013
BACKGROUND AND OBJECTIVE: Interleukin-6 (IL-6) is a key proinflammatory cytokine that has been considered to be important in the pathogenesis of periodontal disease. Therefore, host-modulatory agents directed at inhibiting IL-6 appear to be beneficial in terms of attenuating periodontal disease progression and potentially improving disease susceptibility. In the current study, ...
Lu Quansheng Q Division of Nephrology, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, - - 2013
D5 dopamine receptor (D5R) knock-out mice (D5(-/-)) have a higher blood pressure (BP) and higher reactive oxygen species (ROS) production than their D5R wild-type littermates (D5(+/+)). We tested the hypothesis that the high BP and increased ROS production in D5(-/-) mice may be caused by decreased heme oxygenase-1 (HO-1) expression ...
Clérigues Victoria - - 2013
Heme oxygenase-1 (HO-1) catalyses the degradation of heme and possesses anti-inflammatory and cytoprotective properties. The role of inflammatory mediators in the pathogenesis of osteoarthritis (OA) is becoming increasingly appreciated. Here we investigated the effects of HO-1 induction in OA and healthy human articular chondrocytes in response to inflammatory cytokine interleukin-1β ...
Lin Heng-Huei - - 2013
Increased cardiac stromal cell-derived factor-1α (SDF-1α) expression promotes neovascularization and myocardial repair after ischemic injury through recruiting stem cells and reducing cardiomyocyte death. Previous studies have shown that heme oxygenase-1 and its reaction byproduct, carbon monoxide (CO), induce SDF-1α expression in ischemic heart. However, the mechanism underlying heme oxygenase-1/CO-induced cardiac ...
Kou Mei-Chun - - 2013
SCOPE: Curcumin (CUR), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) have been demonstrated as having antioxidant, anticarcinogenic, and hypocholesterolemic activities. We report the diverse antiatherogenic effects and mechanisms of curcuminoids. METHODS AND RESULTS: We found that CUR was the most potent antioxidant against copper-mediated LDL oxidation as measured by thiobarbituric acid-reactive substances ...
Nie Xiaowei - - 2013
We previously reported that 15-lipoxygenase (15-LO) induced by hypoxia catalyzed the conversion of arachidonic acid (AA) into 15-hydroxyeicosatetraenoic acid (15-HETE), which plays an essential role in the development of hypoxic pulmonary arterial hypertension (HPH). However, the mechanisms by which hypoxia up-regulated 15-LO are still unclear. Heme Oxygenase-1 (HO-1), an oxygen-dependent ...
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