Search Results
Results 1 - 50 of 2386
1 2 3 4 5 6 7 8 9 10 >
Yang Yi-Chieh YC *Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan; †Institute of Basic Medical Science, College of Medicine, National Cheng Kung University, Tainan, Taiwan; ‡Division of Chest Medicine, Department of Internal Medicine, Chi Mei Medical Center, Tainan; The Center of General Education, Chia Nan University of Pharmacy & Science, Tainan, Taiwan; §Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan; and ‖Division of Thoracic Surgery, Taipei Veterans General Hospital; Institute of Emergency and Critical Care Medicine, National Yang-Ming University School of Medicine, Taipei, - - 2014
DNA methyltransferase 3B (DNMT3B) contributes to de novo DNA methylation and its overexpression promotes tumorigenesis. However, whether DNMT3B is upregulated by transcriptional deregulation remains unclear. We studied the transcriptional repression of DNMT3B by forkhead O transcription factor 3a (FOXO3a) in lung cancer cell, animal, and clinical models. The results of ...
Guillot Clément - - 2014
Hepatocellular carcinoma is the third cause of cancer related death for which new treatment strategies are needed. Targeting DNA repair pathways to sensitize tumor cells to chemo- or radiotherapy is under investigation for the treatment of several cancers with poly(ADP-ribose) polymerase (PARP) inhibitors showing great potential. The aim of this ...
Sutinen Päivi P Institute of Biomedicine, University of Eastern Finland, Kuopio (P.S.,V.R., M.R., J.J.P.), Department of Pathology, Kuopio University of Hospital, Kuopio (J.J.P.), - - 2014
FOX (forkhead box) protein A1 has been dubbed a pioneer transcription factor, since it binds target sites in DNA, thereby displacing nucleosomes to loosen chromatin and facilitating steroid receptor DNA binding nearby. FOXA1 is an important regulator of prostate development, collaborating with androgen receptor (AR). Post-translational modifications regulating FOXA1 are ...
Demongeot J J AGIM, FRE CNRS 3405, Faculty of Medicine of Grenoble, University J. Fourier, 38700, La Tronche, France, - - 2014
The problem of stability in population dynamics concerns many domains of application in demography, biology, mechanics and mathematics. The problem is highly generic and independent of the population considered (human, animals, molecules,…). We give in this paper some examples of population dynamics concerning nucleic acids interacting through direct nucleic binding ...
Misaghi Shahram S Department of Early Stage Cell Culture, Genentech, Inc, 1 DNA way, South San Francisco, California, - - 2014
Clonal instability and titer loss during Chinese hamster ovary (CHO) cell line development (CLD) has several underlying causes, the most prominent of which are DNA copy number loss and DNA silencing. However, in some cases, clonal instability is due to the toxicity of the therapeutic protein(s) that clones express. Unlike ...
Lennard Richard Mara L ML Division of Rheumatology and Immunology, Department of Medicine, Medical University of South Carolina, Charleston, SC 29425; - - 2014
The friend leukemia insertion site 1 (Fli-1) transcription factor, an Ets family member, is implicated in the pathogenesis of systemic lupus erythematosus in human patients and murine models of lupus. Lupus-prone mice with reduced Fli-1 expression have significantly less nephritis, prolonged survival, and decreased infiltrating inflammatory cells into the kidney. ...
Lennard Richard Mara L ML Department of Medicine, Division of Rheumatology & Immunology, Medical University of South Carolina, Charleston, SC 29425, - - 2014
Regulation of proinflammatory cytokines and chemokines is a primary role of the innate immune response. MCP-1 is a chemokine that recruits immune cells to sites of inflammation. Expression of MCP-1 is reduced in primary kidney endothelial cells from mice with a heterozygous knockout of the Fli-1 transcription factor. Fli-1 is ...
Chen TieLin T Core Facility of Genetically Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China, - - 2014
High mobility group (HMG)-box proteins, a large and functionally diverse superfamily of architectural protein, are involved in the regulation of DNA-dependent processes such as transcription, replication and DNA repair via the HMG-box domain. Bobby sox homolog (BBX), a newly identified HMG-Box protein, may function as a sequence-specific transcription factor. However, ...
Giammartino Dafne Campigli Di DC Columbia University, Department of Biological Sciences, New York NY, 10027, - - 2014
The 3' ends of most eukaryotic messenger RNAs must undergo a maturation step that includes an endonucleolytic cleavage followed by addition of a polyadenylate tail. While this reaction is catalyzed by the action of only two enzymes it is supported by an unexpectedly large number of proteins. This complexity reflects ...
Lee Alex G AG Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, United - - 2014
Intermittent exposure to mildly stressful situations provides opportunities to practice coping in the context of exposure psychotherapies and stress inoculation training. Previously, we showed that stress inoculation modeled in juvenile monkeys enhances subsequent indications of resilience. Here we examine stress inoculation effects in adult female monkeys. We found that stress ...
Clewell Rebecca A RA The Hamner Institutes for Health Sciences, Research Triangle Park, NC, USA - - 2014
As part of a larger effort to provide proof-of-concept in vitro only risk assessments, we have developed a suite of high throughput assays for key readouts in the p53 DNA damage response toxicity pathway: double strand break DNA damage (p-H2AX), permanent chromosomal damage (micronuclei), p53 activation, p53 transcriptional activity, and ...
Bhattacharyya Debmalya - - 2014
RNA G-quadruplex (GQ) structures act as regulators of diverse array of cellular processes including translation, pre-mRNA processing and mRNA targeting. We report here a strategy of harnessing the natural ability of RNA GQs to inhibit translation by rationally inducing a GQ on a targeted mRNA to knockdown endogenous gene expression. ...
Senturk Serif S Cold Spring Harbor Laboratory Cancer Center, Cold Spring Harbor, NY - - 2014
Although much is known about the underlying mechanisms of p53 activity and regulation, the factors that influence the diversity and duration of p53 responses are not well understood. Here we describe a unique mode of p53 regulation involving alternative splicing of the TP53 gene. We found that the use of ...
Wade Joseph T JT Wadsworth Center, New York State Department of Health, Albany, New York 12208, USA, and the Department of Biomedical Sciences, University at Albany-State University of New York, Albany, New York 12201, - - 2014
The conventional view of transcription posits that mRNAs are generated from the coding DNA strand and are delineated by gene boundaries; however, recent reports have mapped transcription start sites to unexpected locations in bacterial genomes, including the non-coding strand. The resultant RNAs were previously dismissed as artefacts, but models that ...
Glidewell-Kenney Christine A CA Department of Reproductive Medicine and the Center for Reproductive Science and Medicine, University of California, San Diego, LA Jolla, CA - - 2014
Mutations in Neurokinin B (NKB) and its receptor, NK3R, were identified in human patients with hypogonadotropic hypogonadism, a disorder characterized by lack of puberty and infertility. Further studies have suggested NKB acts at the level of the hypothalamus to control GnRH neuron activity, either directly or indirectly. We recently reported ...
Guimarães Douglas Magno DM Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, - - 2014
Odontogenic tumours are a heterogeneous group of lesions formed from tissues that give rise to the tooth. DNA methylation, a covalent addition of a methyl group to the 5-carbon position of a cytosine nucleotide, is considered an important regulator of gene expression. The addition of the methyl radical is catalysed ...
Capell Anja A Ludwig Maximilians University, - - 2014
Frontotemporal lobar degeneration (FTLD) is associated with cytoplasmic or nuclear deposition of the TAR DNA binding protein 43 (TDP-43). Haploinsufficiency of progranulin (GRN) is a major genetic risk for FTLD associated with TDP-43 deposition. Therefore understanding the mechanisms, which control cellular expression of GRN is required not only to understand ...
Ghazaryan Lusine L Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben-Gurion University, 84990, Midreshet Ben-Gurion, - - 2014
Bacteriocins produced by Enterobacteriaceae are high molecular weight toxic proteins that kill target cells through a variety of mechanisms, including pore formation and nucleic acid degradation. What is remarkable about these toxins is that their expression results in death to the producing cells and therefore bacteriocin induction have to be ...
López-Lera Alberto - - 2014
SERPING1 mutations causing Hereditary Angioedema type I (HAE-I) due to C1-Inhibitor (C1-INH) deficiency display a dominant-negative effect usually resulting in protein levels far below the expected 50%. To further investigate mechanisms for its reduced expression, we analyzed the promoter DNA methylation status of SERPING1 and its influence on C1-INH expression. ...
Imagawa Kei K Bone and Joint Research Group, Centre for Human Development Stem Cells and Regeneration, Institute of Developmental Science, University of Southampton Medical School, Southampton, UK; Department of Orthopaedics, Tohoku University Graduate School of Medicine, Sendai, - - 2014
Objective: To investigate whether the changes in collagen gene expression in osteoarthritic (OA) human chondrocytes are associated with changes in the DNA methylation status in the COL2A1 enhancer and COL9A1 promoter. Methods: Expression levels were determined using qRT-PCR and DNA methylation percentage was quantified by pyrosequencing. The effect of CpG ...
Sidler Corinne C Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, T1K 3M4, - - 2014
Cellular senescence has been associated with the age-dependent decline in tissue repair and regeneration, the increasing deterioration of the immune system, and the age-dependent increase in the incidence of cancer. Here, we show that senescence of human lung fibroblast WI-38 cells is associated with extensive changes to the gene expression ...
White H H 1] National Genetics Reference Laboratory (Wessex), Salisbury District Hospital, Salisbury, UK [2] Faculty of Medicine, University of Southampton, Southampton, - - 2014
Serial quantification of BCR-ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukemia, but there is substantial variation in results reported by different laboratories. To improve comparability, an internationally accepted plasmid certified reference material (CRM) was developed according to ISO Guide 34:2009. Fragments of BCR-ABL1 (e14a2 mRNA fusion), BCR ...
Weaver Kelly L KL Surgery, University of - - 2014
The Notch signaling pathway governs many distinct cellular processes by regulating transcriptional programs. The transcriptional response initiated by Notch is highly cell context dependent, indicating that multiple factors influence Notch target gene selection and activity. However, the mechanism by which Notch drives target gene transcription is not well understood. Herein, ...
Zeng Cheng-Wu CW Key Laboratory of Gene Engineering of the Ministry of Education; State Key Laboratory for Biocontrol; School of Life Science; Sun Yat-sen University; Guangzhou, - - 2014
Acute promyelocytic leukemia (APL) is characterized by the t(15;17)-associated PML-RARA fusion gene. We have previously found that MIR125B1 is highly expressed in patients with APL and may be associated with disease pathogenesis; however, the mechanism by which MIR125B1 exerts its oncogenic potential has not been fully elucidated. Here, we demonstrated ...
Chong Shasha S Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, - - 2014
Transcription of highly expressed genes has been shown to occur in stochastic bursts. But the origin of such ubiquitous phenomenon has not been understood. Here, we present the mechanism in bacteria. We developed a high-throughput, in vitro, single-molecule assay to follow transcription on individual DNA templates in real time. We showed ...
Levens David D Center for Cancer Research, National Cancer Institute, Bethesda, MD 20814, - - 2014
Transcriptional bursting has been observed across species and is one of the primary causes of variable gene expression in cells and tissue. In this issue, Chong et al. describe how DNA topology results in transcriptional bursting in E. coli.
Zhou Yi Y State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, PR - - 2014
The Escherichia coli cAMP receptor protein (CRP) activates transcription initiation at many promoters by binding upstream of core promoter elements and interacting with the C-terminal domain of the RNA polymerase α subunit. Previous studies have shown stringent spacing is required for transcription activation by CRP. Here we report that this ...
Ottoz Diana S M DS ETH Zurich and Swiss Institute of Bioinformatics, D-BSSE, Mattenstrasse 26, 4058 Basel, Switzerland Life Science Zurich PhD Program on Molecular and Translational Biomedicine, Zurich, Switzerland Competence Center for Systems Physiology and Metabolic Diseases, ETH Zurich, 8093 Zurich, - - 2014
The precise control of gene expression is essential in basic biological research as well as in biotechnological applications. Most regulated systems available in yeast enable only the overexpression of the target gene, excluding the possibility of intermediate or weak expression. Moreover, these systems are frequently toxic or depend on growth ...
Ratnu Vikram S VS Psychiatric Epigenomics Laboratory, Queensland Brain Institute, The University of Queensland, Brisbane, Qld, 4072, - - 2014
Activity-dependent gene expression depends, in part, on transcriptional regulation that is coordinated by rapid changes in the chromatin landscape as well as the covalent modification of DNA. Here we demonstrate that the expression of brain-derived neurotrophic factor (BDNF), a gene that is critically involved in neural plasticity and subject to ...
Hirata-Tsuchiya Shizu S Department of Health Improvement - - 2014
Bone morphogenic proteins (BMPs) stimulate bone formation in vivo and osteoblast differentiation in vitro via a Smad signaling pathway. Recent findings revealed that the activation of NF-κB inhibits BMP-induced osteoblast differentiation. Here, we show that NF-κB inhibits BMP signaling by directly targeting the Smad pathway. A selective inhibitor of the ...
Sharon Eilon E Weizmann Institute of - - 2014
Genetically identical cells exhibit large variability (noise) in gene expression, with important consequences for cellular function. Although the amount of noise decreases with and is thus partly determined by the mean expression level, the extent to which different promoter sequences can deviate away from this trend is not fully known. ...
Harvey Vivian C VC Department of Biology, The College of William and Mary, Williamsburg VA, - - 2014
The pathogenesis of diseases elicited by the gastric pathogen Helicobacter pylori is partially determined by the effectiveness of adaptation to the variable acidic environment of the host stomach. Adaptation includes appropriate adherence to the gastric epithelium via outer membrane protein adhesins such as SabA. Expression of sabA is subject to ...
Choe Junho J Division of Life Sciences, Korea University, Seoul 136-701, Republic of - - 2014
Histone biogenesis is tightly controlled at multiple steps to maintain the balance between the amounts of DNA and histone protein during the cell cycle. In particular, translation and degradation of replication-dependent histone mRNAs are coordinately regulated. However, the underlying molecular mechanisms remain elusive. Here, we investigate remodeling of stem-loop binding ...
Zhuang Yanyan Y Division of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning, - - 2014
To determine the methylation status of progesterone receptor B (PR-B) promoter and how PR-B regulates progesterone action in placenta during human pregnancy. Placentas were obtained from the pregnancy women at term who underwent cesarean delivery and vaginal delivery. The methylation status of the PR-B promoter was analyzed using the methylation-specific ...
Nam Yoon Seok YS Medical Research Center for Gene Regulation, Chonnam National University Medical - - 2014
Rationale: Small heterodimer partner (SHP; NR0B2) is an atypical orphan nuclear receptor that lacks a conventional DNA binding domain. Through interactions with other transcription factors, SHP regulates diverse biological events, including glucose metabolism in liver. However, the role of SHP in adult heart diseases has not yet been demonstrated. Objective: ...
Xie Qiguang Q Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Sciences, Hebei Normal University, Hebei Collaboration Innovation Center for Cell Signaling, Shijiazhuang, Hebei 050024, - - 2014
Transcriptional feedback loops are central to the architecture of eukaryotic circadian clocks. Models of the Arabidopsis thaliana circadian clock have emphasized transcriptional repressors, but recently, Myb-like REVEILLE (RVE) transcription factors have been established as transcriptional activators of central clock components, including PSEUDO-RESPONSE REGULATOR5 (PRR5) and TIMING OF CAB EXPRESSION1 (TOC1). ...
Turapov Obolbek O University of Leicester, United - - 2014
Regulator of Antimicrobial-Assisted Survival), encoded by Rv1219c in Mycobacterium tuberculosis and bcg_1279c in Mycobacterium bovis BCG, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and to lesser extent palmitoyl-CoA) modulate RaaS binding to DNA ...
Wang Zhongchuan Z Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, - - 2014
MicroRNAs (miRNAs) are short, non-coding RNAs that regulate the expression of multiple target genes. Dysregulation of miRNAs is common in sepsis. Through microRNA microarray and qRT-PCR we found that the levels of miR-27a, miR-153 and miR-143 are up regulated, while let-7a, miR-218 and miR-129-5p are down regulated in lungs of ...
Yang Zu Z Beijing Key Laboratory of Animal Genetic Improvement & Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China, - - 2014
The high glycine/tyrosine type II keratin protein 6.1 (KAP6.1) is a member of the keratin-associated protein family, which is restricted to cells in hair follicles and is associated with fiber diameter and fiber curvature in Merino sheep. In this study, we obtained a series of progressive 5'-deletion fragments of the ...
Palsamy Periyasamy P Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, Omaha, NE 68198, USA. Electronic address: - - 2014
Cataract-induced by sodium selenite in suckling rats is one of the suitable animal models to study the basic mechanism of human cataracts formation. The aim of this present investigation is to study the endoplasmic reticulum (ER) stress-mediated activation of unfolded protein response (UPR), overproduction of reactive oxygen species (ROS), and ...
Liu Lin-Xin LX Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, - - 2014
To explore tissue factor (TF) expression and methylation regulation in differentiation of human embryonic stem cells (hESCs) into trophoblast. Differentiation of hESCs into trophoblast was induced by bone morphogenetic protein 4 (BMP4). Expression of gene, protein of TF and DNA methylation at different time points during induction process was detected ...
Takayama Koji K Stem Cell Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, 15219; Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, - - 2014
DNA damage is a cause of age related pathologies, including osteoarthritis (OA). Excision repair cross complementation group 1 (ERCC1) is an endonuclease required for DNA damage repair. In this study we investigated the function of ERCC1 in chondrocytes and its association with the pathophysiology of OA. ERCC1 expression in normal ...
Senecal Adrien A Functional Imaging of Transcription, Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), and Inserm U1024, and CNRS UMR 8197, Paris 75005, France; Transcription Imaging Consortium, Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA; UPMC University Paris 06, 75005 Paris, - - 2014
Transcription is a stochastic process occurring mostly in episodic bursts. Although the local chromatin environment is known to influence the bursting behavior on long timescales, the impact of transcription factors (TFs)-especially in rapidly inducible systems-is largely unknown. Using fluorescence in situ hybridization and computational models, we quantified the transcriptional activity of ...
Mahindhoratep S S U1022 INSERM; UMR8258 CNRS; Unité des Techniques Chimiques et Biologiques pour la Santé; Chimie ParisTech; Faculté de Pharmacie 4 Avenue de l'Observatoire, 75006 Paris, - - 2014
When activated, NF-κB can promote the nuclear import and transcription of DNA possessing NF-κB consensus sequences. Here, we investigated whether NF-κB is involved in the plasmid electrotransfer process. Mouse tibial cranial muscles were transfected with plasmids encoding luciferase bearing or not NF-κB consensus sequences. Luciferase transgene expression was evaluated noninvasively ...
Hwang Ji-Sun JS Department of Physiology and Biophysics, College of Medicine, Inha University, Incheon, - - 2014
The objective of this study is to investigate glucosamine (GlcN) as transcriptional regulator of iNOS and other genes in association with the dynamic O-GlcNAcylation of RNA polymerase II (RNAPII). The LPS- and/or GlcN-stimulated transcriptional activities of various Gal4-binding site/TATA-box-containing reporter constructs were measured. Basal transcriptional activities of nuclear factor-κB (NF-κB) ...
Goto Akira A INSERM, CNRS-UPR9022, France; - - 2014
The host defense of the model organism Drosophila is under the control of two major signaling cascades controlling transcription factors of the NF-κB family, the Toll and the IMD pathways. The latter shares extensive similarities with the mammalian TNF-R pathway and was initially discovered for its role in anti-Gram-negative bacterial ...
Cardamone M Dafne MD Biochemistry Department, Boston University School of Medicine, 72 East Concord Street, Boston, MA 02118, - - 2014
Timely and selective recruitment of transcription factors to their appropriate DNA-binding sites represents a critical step in regulating gene activation; however, the regulatory strategies underlying each factor's effective recruitment to specific promoter and/or enhancer regions are not fully understood. Here, we identify an unexpected regulatory mechanism by which promoter-specific binding, ...
Sofie Vandevyver V Inflammation Research Center, Flanders Institute for Biotechnology (VIB), B9052 Ghent, - - 2014
Glucocorticoids are among the most prescribed drugs worldwide for the treatment of numerous immune and inflammatory disorders. They exert their actions by binding to the glucocorticoid receptor (GR), a member of the nuclear receptor superfamily. There are several GR isoforms resulting from alternative RNA splicing and translation initiation of the ...
Castro-Diaz Nathaly N School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, - - 2014
Mobile elements are important evolutionary forces that challenge genomic integrity. Long interspersed element-1 (L1, also known as LINE-1) is the only autonomous transposon still active in the human genome. It displays an unusual pattern of evolution, with, at any given time, a single active L1 lineage amplifying to thousands of ...
Ma Tai-Yang TY State Key Laboratory Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, P.R. - - 2014
FasL is the most extensively studied apoptosis ligand. In 2000, tilapia FasL was identified using anti-human FasL monoclonal antibody by Evans's research group. Recently, a tilapia FasL-like protein of smaller molecule weight was predicted in Genbank (XM_003445156.2). Based on several clues drawn from previous studies, we cast doubt on the ...
1 2 3 4 5 6 7 8 9 10 >