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Glidewell-Kenney Christine A CA Department of Reproductive Medicine and the Center for Reproductive Science and Medicine, University of California, San Diego, LA Jolla, CA - - 2014
Mutations in Neurokinin B (NKB) and its receptor, NK3R, were identified in human patients with hypogonadotropic hypogonadism, a disorder characterized by lack of puberty and infertility. Further studies have suggested NKB acts at the level of the hypothalamus to control GnRH neuron activity, either directly or indirectly. We recently reported ...
Guimarães Douglas Magno DM Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, - - 2014
Odontogenic tumours are a heterogeneous group of lesions formed from tissues that give rise to the tooth. DNA methylation, a covalent addition of a methyl group to the 5-carbon position of a cytosine nucleotide, is considered an important regulator of gene expression. The addition of the methyl radical is catalysed ...
Capell Anja A Ludwig Maximilians University, - - 2014
Frontotemporal lobar degeneration (FTLD) is associated with cytoplasmic or nuclear deposition of the TAR DNA binding protein 43 (TDP-43). Haploinsufficiency of progranulin (GRN) is a major genetic risk for FTLD associated with TDP-43 deposition. Therefore understanding the mechanisms, which control cellular expression of GRN is required not only to understand ...
Ghazaryan Lusine L Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben-Gurion University, 84990, Midreshet Ben-Gurion, - - 2014
Bacteriocins produced by Enterobacteriaceae are high molecular weight toxic proteins that kill target cells through a variety of mechanisms, including pore formation and nucleic acid degradation. What is remarkable about these toxins is that their expression results in death to the producing cells and therefore bacteriocin induction have to be ...
López-Lera Alberto - - 2014
SERPING1 mutations causing Hereditary Angioedema type I (HAE-I) due to C1-Inhibitor (C1-INH) deficiency display a dominant-negative effect usually resulting in protein levels far below the expected 50%. To further investigate mechanisms for its reduced expression, we analyzed the promoter DNA methylation status of SERPING1 and its influence on C1-INH expression. ...
Imagawa Kei K Bone and Joint Research Group, Centre for Human Development Stem Cells and Regeneration, Institute of Developmental Science, University of Southampton Medical School, Southampton, UK; Department of Orthopaedics, Tohoku University Graduate School of Medicine, Sendai, - - 2014
Objective: To investigate whether the changes in collagen gene expression in osteoarthritic (OA) human chondrocytes are associated with changes in the DNA methylation status in the COL2A1 enhancer and COL9A1 promoter. Methods: Expression levels were determined using qRT-PCR and DNA methylation percentage was quantified by pyrosequencing. The effect of CpG ...
Sidler Corinne C Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, T1K 3M4, - - 2014
Cellular senescence has been associated with the age-dependent decline in tissue repair and regeneration, the increasing deterioration of the immune system, and the age-dependent increase in the incidence of cancer. Here, we show that senescence of human lung fibroblast WI-38 cells is associated with extensive changes to the gene expression ...
White H H 1] National Genetics Reference Laboratory (Wessex), Salisbury District Hospital, Salisbury, UK [2] Faculty of Medicine, University of Southampton, Southampton, - - 2014
Serial quantification of BCR-ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukemia, but there is substantial variation in results reported by different laboratories. To improve comparability, an internationally accepted plasmid certified reference material (CRM) was developed according to ISO Guide 34:2009. Fragments of BCR-ABL1 (e14a2 mRNA fusion), BCR ...
Weaver Kelly L KL Surgery, University of - - 2014
The Notch signaling pathway governs many distinct cellular processes by regulating transcriptional programs. The transcriptional response initiated by Notch is highly cell context dependent, indicating that multiple factors influence Notch target gene selection and activity. However, the mechanism by which Notch drives target gene transcription is not well understood. Herein, ...
Chong Shasha S Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, - - 2014
Transcription of highly expressed genes has been shown to occur in stochastic bursts. But the origin of such ubiquitous phenomenon has not been understood. Here, we present the mechanism in bacteria. We developed a high-throughput, in vitro, single-molecule assay to follow transcription on individual DNA templates in real time. We showed ...
Levens David D Center for Cancer Research, National Cancer Institute, Bethesda, MD 20814, - - 2014
Transcriptional bursting has been observed across species and is one of the primary causes of variable gene expression in cells and tissue. In this issue, Chong et al. describe how DNA topology results in transcriptional bursting in E. coli.
Zhou Yi Y State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, PR - - 2014
The Escherichia coli cAMP receptor protein (CRP) activates transcription initiation at many promoters by binding upstream of core promoter elements and interacting with the C-terminal domain of the RNA polymerase α subunit. Previous studies have shown stringent spacing is required for transcription activation by CRP. Here we report that this ...
Ottoz Diana S M DS ETH Zurich and Swiss Institute of Bioinformatics, D-BSSE, Mattenstrasse 26, 4058 Basel, Switzerland Life Science Zurich PhD Program on Molecular and Translational Biomedicine, Zurich, Switzerland Competence Center for Systems Physiology and Metabolic Diseases, ETH Zurich, 8093 Zurich, - - 2014
The precise control of gene expression is essential in basic biological research as well as in biotechnological applications. Most regulated systems available in yeast enable only the overexpression of the target gene, excluding the possibility of intermediate or weak expression. Moreover, these systems are frequently toxic or depend on growth ...
Ratnu Vikram S VS Psychiatric Epigenomics Laboratory, Queensland Brain Institute, The University of Queensland, Brisbane, Qld, 4072, - - 2014
Activity-dependent gene expression depends, in part, on transcriptional regulation that is coordinated by rapid changes in the chromatin landscape as well as the covalent modification of DNA. Here we demonstrate that the expression of brain-derived neurotrophic factor (BDNF), a gene that is critically involved in neural plasticity and subject to ...
Hirata-Tsuchiya Shizu S Department of Health Improvement - - 2014
Bone morphogenic proteins (BMPs) stimulate bone formation in vivo and osteoblast differentiation in vitro via a Smad signaling pathway. Recent findings revealed that the activation of NF-κB inhibits BMP-induced osteoblast differentiation. Here, we show that NF-κB inhibits BMP signaling by directly targeting the Smad pathway. A selective inhibitor of the ...
Sharon Eilon E Weizmann Institute of - - 2014
Genetically identical cells exhibit large variability (noise) in gene expression, with important consequences for cellular function. Although the amount of noise decreases with and is thus partly determined by the mean expression level, the extent to which different promoter sequences can deviate away from this trend is not fully known. ...
Harvey Vivian C VC Department of Biology, The College of William and Mary, Williamsburg VA, - - 2014
The pathogenesis of diseases elicited by the gastric pathogen Helicobacter pylori is partially determined by the effectiveness of adaptation to the variable acidic environment of the host stomach. Adaptation includes appropriate adherence to the gastric epithelium via outer membrane protein adhesins such as SabA. Expression of sabA is subject to ...
Choe Junho J Division of Life Sciences, Korea University, Seoul 136-701, Republic of - - 2014
Histone biogenesis is tightly controlled at multiple steps to maintain the balance between the amounts of DNA and histone protein during the cell cycle. In particular, translation and degradation of replication-dependent histone mRNAs are coordinately regulated. However, the underlying molecular mechanisms remain elusive. Here, we investigate remodeling of stem-loop binding ...
Zhuang Yanyan Y Division of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning, - - 2014
To determine the methylation status of progesterone receptor B (PR-B) promoter and how PR-B regulates progesterone action in placenta during human pregnancy. Placentas were obtained from the pregnancy women at term who underwent cesarean delivery and vaginal delivery. The methylation status of the PR-B promoter was analyzed using the methylation-specific ...
Nam Yoon Seok YS Medical Research Center for Gene Regulation, Chonnam National University Medical - - 2014
Rationale: Small heterodimer partner (SHP; NR0B2) is an atypical orphan nuclear receptor that lacks a conventional DNA binding domain. Through interactions with other transcription factors, SHP regulates diverse biological events, including glucose metabolism in liver. However, the role of SHP in adult heart diseases has not yet been demonstrated. Objective: ...
Xie Qiguang Q Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Sciences, Hebei Normal University, Hebei Collaboration Innovation Center for Cell Signaling, Shijiazhuang, Hebei 050024, - - 2014
Transcriptional feedback loops are central to the architecture of eukaryotic circadian clocks. Models of the Arabidopsis thaliana circadian clock have emphasized transcriptional repressors, but recently, Myb-like REVEILLE (RVE) transcription factors have been established as transcriptional activators of central clock components, including PSEUDO-RESPONSE REGULATOR5 (PRR5) and TIMING OF CAB EXPRESSION1 (TOC1). ...
Turapov Obolbek O University of Leicester, United - - 2014
Regulator of Antimicrobial-Assisted Survival), encoded by Rv1219c in Mycobacterium tuberculosis and bcg_1279c in Mycobacterium bovis BCG, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and to lesser extent palmitoyl-CoA) modulate RaaS binding to DNA ...
Wang Zhongchuan Z Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, - - 2014
MicroRNAs (miRNAs) are short, non-coding RNAs that regulate the expression of multiple target genes. Dysregulation of miRNAs is common in sepsis. Through microRNA microarray and qRT-PCR we found that the levels of miR-27a, miR-153 and miR-143 are up regulated, while let-7a, miR-218 and miR-129-5p are down regulated in lungs of ...
Yang Zu Z Beijing Key Laboratory of Animal Genetic Improvement & Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China, - - 2014
The high glycine/tyrosine type II keratin protein 6.1 (KAP6.1) is a member of the keratin-associated protein family, which is restricted to cells in hair follicles and is associated with fiber diameter and fiber curvature in Merino sheep. In this study, we obtained a series of progressive 5'-deletion fragments of the ...
Palsamy Periyasamy P Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, Omaha, NE 68198, USA. Electronic address: - - 2014
Cataract-induced by sodium selenite in suckling rats is one of the suitable animal models to study the basic mechanism of human cataracts formation. The aim of this present investigation is to study the endoplasmic reticulum (ER) stress-mediated activation of unfolded protein response (UPR), overproduction of reactive oxygen species (ROS), and ...
Liu Lin-Xin LX Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, - - 2014
To explore tissue factor (TF) expression and methylation regulation in differentiation of human embryonic stem cells (hESCs) into trophoblast. Differentiation of hESCs into trophoblast was induced by bone morphogenetic protein 4 (BMP4). Expression of gene, protein of TF and DNA methylation at different time points during induction process was detected ...
De Pauw A A Saint-Luc University Clinics, Brussels, - - 2014
Epigenetic programming within the cardiac stem cell (CSC) niche controls CSC specification and differentiation, but the identity of putative mediators is poorly characterized. To dissect these mechanisms, we used primary and clonally expanded Sca-1+/c-kit+ CSC from murine adult hearts and stimulated cardiomyocyte differentiation upon culture in a differentiation medium (DIFF) ...
Zhou W W Imperial College London, National Heart and Lung Institute, London, United - - 2014
G-quadruplexes (G4s) are four-stranded DNA secondary structures involved in a diverse range of biological processes. We have previously revealed that G4s are important in cardiac function-related genes and pathways. In the beta-adrenergic receptor signaling pathway, which is directly involved in heart failure (HF), conserved G4s have been found in the ...
Takayama Koji K Stem Cell Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, 15219; Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, - - 2014
DNA damage is a cause of age related pathologies, including osteoarthritis (OA). Excision repair cross complementation group 1 (ERCC1) is an endonuclease required for DNA damage repair. In this study we investigated the function of ERCC1 in chondrocytes and its association with the pathophysiology of OA. ERCC1 expression in normal ...
Senecal Adrien A Functional Imaging of Transcription, Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), and Inserm U1024, and CNRS UMR 8197, Paris 75005, France; Transcription Imaging Consortium, Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA; UPMC University Paris 06, 75005 Paris, - - 2014
Transcription is a stochastic process occurring mostly in episodic bursts. Although the local chromatin environment is known to influence the bursting behavior on long timescales, the impact of transcription factors (TFs)-especially in rapidly inducible systems-is largely unknown. Using fluorescence in situ hybridization and computational models, we quantified the transcriptional activity of ...
Mahindhoratep S S U1022 INSERM; UMR8258 CNRS; Unité des Techniques Chimiques et Biologiques pour la Santé; Chimie ParisTech; Faculté de Pharmacie 4 Avenue de l'Observatoire, 75006 Paris, - - 2014
When activated, NF-κB can promote the nuclear import and transcription of DNA possessing NF-κB consensus sequences. Here, we investigated whether NF-κB is involved in the plasmid electrotransfer process. Mouse tibial cranial muscles were transfected with plasmids encoding luciferase bearing or not NF-κB consensus sequences. Luciferase transgene expression was evaluated noninvasively ...
Hwang Ji-Sun JS Department of Physiology and Biophysics, College of Medicine, Inha University, Incheon, - - 2014
The objective of this study is to investigate glucosamine (GlcN) as transcriptional regulator of iNOS and other genes in association with the dynamic O-GlcNAcylation of RNA polymerase II (RNAPII). The LPS- and/or GlcN-stimulated transcriptional activities of various Gal4-binding site/TATA-box-containing reporter constructs were measured. Basal transcriptional activities of nuclear factor-κB (NF-κB) ...
Goto Akira A INSERM, CNRS-UPR9022, France; - - 2014
The host defense of the model organism Drosophila is under the control of two major signaling cascades controlling transcription factors of the NF-κB family, the Toll and the IMD pathways. The latter shares extensive similarities with the mammalian TNF-R pathway and was initially discovered for its role in anti-Gram-negative bacterial ...
Cardamone M Dafne MD Biochemistry Department, Boston University School of Medicine, 72 East Concord Street, Boston, MA 02118, - - 2014
Timely and selective recruitment of transcription factors to their appropriate DNA-binding sites represents a critical step in regulating gene activation; however, the regulatory strategies underlying each factor's effective recruitment to specific promoter and/or enhancer regions are not fully understood. Here, we identify an unexpected regulatory mechanism by which promoter-specific binding, ...
Sofie Vandevyver V Inflammation Research Center, Flanders Institute for Biotechnology (VIB), B9052 Ghent, - - 2014
Glucocorticoids are among the most prescribed drugs worldwide for the treatment of numerous immune and inflammatory disorders. They exert their actions by binding to the glucocorticoid receptor (GR), a member of the nuclear receptor superfamily. There are several GR isoforms resulting from alternative RNA splicing and translation initiation of the ...
Castro-Diaz Nathaly N School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, - - 2014
Mobile elements are important evolutionary forces that challenge genomic integrity. Long interspersed element-1 (L1, also known as LINE-1) is the only autonomous transposon still active in the human genome. It displays an unusual pattern of evolution, with, at any given time, a single active L1 lineage amplifying to thousands of ...
Ma Tai-Yang TY State Key Laboratory Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, P.R. - - 2014
FasL is the most extensively studied apoptosis ligand. In 2000, tilapia FasL was identified using anti-human FasL monoclonal antibody by Evans's research group. Recently, a tilapia FasL-like protein of smaller molecule weight was predicted in Genbank (XM_003445156.2). Based on several clues drawn from previous studies, we cast doubt on the ...
Salzer Ralf R Molecular Microbiology & Bioenergetics, Institute of Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, - - 2014
The thermophilic bacterium Thermus thermophilus HB27 is known for its highly efficient natural transformation system, which has become a model system to study the structure and function of DNA transporter in thermophilic bacteria. The DNA transporter is functionally linked to type IV pili (T4P), which are essential for twitching motility ...
Giacomodonato Mónica N MN Instituto de Investigaciones en Microbiología y Parasitología Médica, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET). Facultad de Medicina, Paraguay 2155, p12, C1121ABG, Buenos Aires, Argentina. Electronic address: - - 2014
DNA adenine methylation is an essential factor in Salmonella virulence. Here, we investigate the involvement of DNA adenine methylase (Dam) in the expression and translocation of a SPI-5-encoded effector of S. Typhimurium. SopB expression and secretion were determined using SopB-FLAG-tagged wild type and dam strains of S. Typhimurium. Western blot ...
Zhou Min M 1 MOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology, and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University , Shanghai, China - - 2014
Abstract Salmonella is the most common cause of bacterial food poisoning in humans worldwide. Thus, rapid and reliable methods for the detection of this pathogen are required. Real-time reverse-transcriptase polymerase chain reaction (rt-RT-PCR), which detects the presence of mRNA (shorter half-life than DNA) has shown great potential for detecting viable ...
Xia Chuan C State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China; Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture, The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, - - 2014
In flowering plants, male gametes (sperm cells) develop within male gametophytes (pollen grains) and are delivered to female gametes for double fertilization by pollen tubes. Therefore, pollen tube growth is crucial for reproduction. The mechanisms that control pollen tube growth remain poorly understood. In this study, we demonstrated that the ...
Krivoruchko Anastasia A Institute of Biochemistry and Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario, Canada K1S 5B6. Electronic address: - - 2014
ChREBP (Carbohydrate Response Element Binding Protein) is a glucose-responsive transcription factor that is known to be an important regulator of glycolytic and lipogenic genes in response to glucose. We hypothesized that activation of ChREBP could be relevant to anoxia survival by the anoxia-tolerant turtle, Trachemys scripta elegans. Expression of ChREBP ...
Kamino Yoshitaka Y Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, - - 2014
Human β-defensin-2 (hBD-2) is a type of epithelial antimicrobial peptide. The expression level of hBD-2 mRNA is lower in oral carcinoma cells (OCCs) than in healthy oral epithelium. Yet, it is still unknown how hBD-2 expression is downregulated in OCCs. The present study investigated DNA hypermethylation of hBD-2 in OCCs ...
Bollinger Lance M LM East Carolina - - 2014
Muscle-specific RING Finger-1 (MuRF-1), a ubiquitin ligase and key regulator of proteasome-dependent protein degradation, is highly expressed during skeletal muscle atrophy. The transcription factor, Forkhead box O3 (FoxO3), induces MuRF-1 expression; but the direct role of other major atrophy-related transcription factors, such as SMAD3, is largely unknown. The goal of ...
Ye Jing J Emergency Department, Pôle Sino-Français de Recherches en Sciences du Vivant et Génomique, Shanghai Ruijin Hospital, Shanghai 200025, - - 2014
Telomeres protect chromosome ends from degradation and inappropriate DNA damage response activation through their association with specific factors. Interestingly, these telomeric factors are able to localize outside telomeric regions, where they can regulate the transcription of genes involved in metabolism, immunity and differentiation. These findings delineate a signalling pathway by ...
Eckler Matthew J MJ Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, CA, - - 2014
Fezf1 and Fezf2 are highly conserved transcription factors that were first identified by their specific expression in the anterior neuroepithelium of Xenopus and zebrafish embryos. These proteins share an N-terminal domain with homology to the canonical engrailed repressor motif and a C-terminal DNA binding domain containing six C2H2 zinc-finger repeats. ...
Grzechnik Pawel P Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, - - 2014
Bidirectional promoters are a common feature of many eukaryotic organisms from yeast to humans. RNA Polymerase II that is recruited to this type of promoter can start transcribing in either direction using alternative DNA strands as the template. Such promiscuous transcription can lead to the synthesis of unwanted transcripts that ...
Cao Ji-Xiang JX Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Xue Yuan Road 38, Beijing 100191, PR - - 2014
DNA damage may regulate microRNA (miRNA) biosynthesis at the levels of miRNA transcription, processing and maturation. Although involvement of E2F1 in the regulation of miRNA gene activation in response to DNA damage has been documented, little is known about the role of E2F1 in miRNA processing. In this study we ...
Durairaj Geetha G Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL-62901, - - 2014
Sus1p is a common component of transcriptional co-activator, SAGA (Spt-Ada-Gcn5-Acetyltransferase), and mRNA export complex, TREX-2 (Transcription-export 2), and is involved in promoting transcription as well as mRNA export. However, it is not clearly understood how Sus1p promotes transcription. Here, we show that Sus1p is predominantly recruited to the upstream activating ...
Shi Junwei J Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA; Molecular and Cellular Biology Program, Stony Brook University, Stony Brook, NY 11794, - - 2014
The bromodomain and extraterminal (BET) protein Brd4 recruits transcriptional regulatory complexes to acetylated chromatin. While Brd4 is considered to be a general transcriptional regulator, pharmacological inhibition of BET proteins shows therapeutic activity in a variety of different pathologies, particularly in models of cancer and inflammation. Such effects have been attributed ...
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