Search Results
Results 301 - 350 of 1627
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Ouji Yukiteru - - 2010
A method for obtaining mouse hepatocytes by infusing collagenase solution into the left ventricle was established. This technique was shown to be equivalent to the intra-portal infusion method and more practical, especially in postnatal mice with a small body size.
Arata Ken-Ichi - - 2010
The infusion of a cardioplegic solution is the standard method of myocardial protection during open heart surgery. However, this method interrupts the surgical procedure and it is difficult to ensure a bloodless surgical field. To address these problems, the effect of continuous retrograde gaseous oxygen persufflation (ROP) on myocardial protection ...
Thielmann Matthias - - 2010
Remote ischemic preconditioning (RIPC) with transient upper limb ischemia reduces myocardial injury in patients undergoing on-pump coronary artery bypass grafting (CABG) with cross-clamp fibrillation or blood cardioplegia for myocardial protection. Whether or not such protection is still operative when standard crystalloid cardioplegic arrest is used is uncertain. Fifty-three consecutive, non-diabetic ...
Lin Hui - - 2010
BACKGROUND: Cardiac surgery during an empty beating heart state has proven to be beneficial in myocardial protection. Based on this, we hypothesized that maintaining this state for donor heart preservation would have the same efficacy and a prolonged preservation period. METHODS: Part 1: 12 pigs were divided into two groups ...
Repse Stephen - - 2010
BACKGROUND: This study was conducted in donor hearts obtained after cardiocirculatory death (DCD) to determine whether pre-reperfusion cardioplegia, followed by warm blood perfusion, is superior to cold storage in preserving function sufficient for transplantation. METHODS: Greyhound dogs (n = 15) were anesthetized and cardiocirculatory death and circulatory failure was induced ...
Jacobs Steven - - 2010
PURPOSE OF REVIEW: The problem of organ shortage continuously emphasizes the importance of proper donor management and selection, organ preservation and recipient selection and treatment. This review summarizes state of the art of cardiac allograft preservation with special regard to recent clinical and experimental findings. RECENT FINDINGS: Over the past ...
Sloots Kathryn L - - 2010
OBJECTIVE: Depolarizing potassium cardioplegia does not afford optimal cardioprotection in pediatric or adult patients requiring complicated operative procedures. Polarizing adenosine-lidocaine cardioplegia has been shown to be cardioprotective without hyperkalemia. Our aim was to examine the effects of changing extracellular potassium levels in adenosine-lidocaine cardioplegia on arrest and reanimation properties. METHODS: ...
Huang Han - - 2010
OBJECTIVE: The purpose of this study was to investigate whether adding emulsified isoflurane to St Thomas cardioplegia solution could enhance the cardiac protection after cardioplegic arrest in rats. DESIGN: A randomized, blind study. SETTING: A university laboratory. PARTICIPANTS: Thirty male Sprague-Dawley rats. INTERVENTIONS: Thirty isolated heart preparations were randomly divided ...
D'Onofrio Augusto - - 2010
The aim of this study is to compare outcomes of patients undergoing surgical ventricular reconstruction (SVR) with normothermic cardiopulmonary bypass (CPB) and beating heart or hypothermic CPB and cardioplegic arrest. Between 2001 and 2008, 588 patients underwent SVR. A propensity score matching was performed and 91 matched pairs were created: ...
Chenoune Mourad - - 2010
STUDY AIM: Total liquid ventilation (TLV) with cooled perfluorocarbons has been demonstrated to induce an ultrafast cardioprotective cooling in rabbits. However, it remains unknown whether this technically challenging strategy would be actually more potent than a conventional external cooling after a prolonged ischemia inducing transmural myocardial infarction. METHODS: Anesthetized rabbits ...
Osipov Robert M - - 2010
We investigated the impact of hydrogen sulfide (H(2)S) on myocardium in the setting of cold crystalloid cardioplegia and cardiopulmonary bypass (CP/CPB). Eighteen male Yorkshire pigs underwent 1 h CP/CPB followed by 2 h of reperfusion. Pigs received either: placebo (control, n=9), or H(2)S (as NaHS) as a bolus/infusion (bolus/infusion, n=6), ...
Amit Ben Hayman - - 2010
A 34-year-old woman presented to the emergency department with severe dyspnoea 10 days following a normal-course caesarean delivery. She had been experiencing shortness of breath throughout the third trimester of pregnancy accompanied by tachycardia (110 bpm); however, her evaluation did not include ECG or chest radiography to elucidate the cause. ...
Garg Pankaj - - 2010
We present a case of acute myocardial infarction in a young adult with a history of anabolic steroid abuse. On diagnostic coronary angiography and intravascular ultrasound, he was found to have a distal left main stem thrombus extending into the proximal left anterior descending artery and a large intermediate vessel. ...
Borowski Andreas - - 2010
In 12 patients undergoing elective myocardial revascularization with intermittent administration of warm-blood cardioplegic solution for myocardial protection, we analyzed metabolic changes by assay of global ischemia indicators (pH, lactate, glucose, and potassium), which we measured in the coronary sinus and arterial blood during the ischemic and postischemic periods. A typical ...
Usta Engin - - 2010
After coronary artery bypass grafting ischemia/reperfusion injury inducing cardiomyocyte apoptosis may occur. This surgery-related inflammatory reaction appears to be of extreme complexity with regard to its molecular, cellular and tissue mechanisms and many studies have been performed on animal models. However, finding retrieved from animal studies were only partially confirmed ...
Karahan S Caner - - 2010
BACKGROUND: The aims of this preliminary study were to determine the alteration of serum ischemia-modified albumin (IMA) levels and to investigate whether IMA may be used as an indicator of the cardioprotective efficacy of N-acetylcysteine (NAC) in patients undergoing coronary bypass grafting (CABG). PATIENTS AND METHODS: Forty-four patients were randomized ...
Jin Zhenxiao - - 2009
BACKGROUND: One of the most important factors restricting heart transplantation is the limited myocardial ischemia time. This study investigated the effects of urethane on the hypothermic preservation of donor rat hearts. MATERIALS AND METHODS: Hearts isolated from rats were divided into 2 groups (n = 8), a control group with ...
Koch Achim - - 2010
During cold storage of donor hearts, reactive oxygen species produced by intracellular redox-active chelatable iron potentially alter myocardial function. To reduce this cold-induced injury we investigated the efficacy of two new modifications of the well established histidine-tryptophan-ketogluterate (HTK) solution (Custodiol) with the addition of N-alpha-acetyl-l-histidine and iron-chelators in a heterotopic ...
Fan Ye - - 2010
Much controversy persists regarding the optimal techniques for myocardial protection during heart surgery. Numerous studies have compared warm cardioplegia with cold cardioplegia for myocardial preservation, but the outcomes were inconclusive. The aim of this meta-analysis of randomised controlled trials (RCTs) was to compare the beneficial and harmful effects of warm ...
Tang Xian N - - 2010
Hypothermia is a well established cytoprotectant, with remarkable and consistent effects demonstrated across multiple laboratories. At the clinical level, it has recently been shown to improve neurological outcome following cardiac arrest and neonatal hypoxia-ischemia. It is increasingly being embraced by the medical community, and could be considered an effective neuroprotectant. ...
Ko Yoshihiro - - 2009
Phosphodiesterase (PDE) III inhibitors have been reported in various cellular protective activities via the cyclic adenosine monophosphate (cAMP) pathway. We investigated the effects of amrinone on ischemia/reperfusion injury and intracellular calcium (Ca2+) handling if utilized as a component of terminal warm blood cardioplegia (TWBCP). Anesthetized pig hearts were subjected to ...
Ghomeshi Hooman R - - 2009
Objectives: Reperfusion of ischemic hearts with warm, substrate-enriched, blood cardioplegia may alleviate post-ischemic metabolic and functional derangements. This study investigates this possibility using (31)P magnetic resonance (MR) spectroscopy. Methods: Fifteen blood-perfused Langendorff pig hearts were subjected to 30 min of total, normothermic ischemia. Control hearts (n=8) were reperfused with blood ...
Scarci Marco - - 2009
A best evidence topic in cardiac surgery was written according to a structured protocol. The question addressed was: does intermittent cross-clamp fibrillation provide equivalent myocardial protection compared to cardioplegia in patients undergoing bypass graft revascularisation? Altogether, 58 papers were found using the reported search, of which 13 represented the best ...
Braathen Bj?rn - - 2010
OBJECTIVES: Cardiac arrest during cardiac surgery is most commonly induced by cold blood or cold crystalloid cardioplegia. The results from clinical studies are divergent regarding which of the 2 solutions provides better myocardial protection. This might be explained by several factors. Both heterogeneity in disease for the included patients and ...
Aarsaether Erling - - 2009
The study compares the single dose histidine-tryptophan-ketoglutarate (HTK) cardioplegia to the repeatedly delivered St Thomas' Hospital Solution (STHS) with respect to preservation of left ventricular mechanoenergetics and leakage of troponin T in a porcine experimental model. Fourteen pigs were randomized to a single infusion of 30 ml/kg HTK cardioplegia (n=7) ...
Koch A - - 2009
OBJECTIVE: Effective myocardial preservation is an important condition for cardiac surgery, especially in heart transplantation with long ischemia times. During ischemia and reperfusion, myocardial function is altered by cold-induced ischemic injury. Cold-induced ischemic injury is triggered by cold storage and the amino acid histidine, a main component of the storage ...
De Paola Nico - - 2009
Left isomerism, also called polysplenia, is a laterality disturbance associated with with paired leftsidedness viscera and multiple small spleens. Left isomerism, heart congenital abnormalities and gastrointestinal malformation are strongly associated. We present a case of prenatal diagnosis of left isomerism in a fetus with a structurally normal heart. Left isomerism ...
Pearl Jeffrey M - - 2011
Glucocorticoids can reduce myocardial dysfunction associated with ischemia and reperfusion injury following cardiopulmonary bypass (CPB) and circulatory arrest. The hypothesis was that maintenance of cardiac function after CPB with methylprednisolone therapy results, in part, from preservation of myocyte calcium cycling. Piglets (5-7 kg) underwent CPB and 120 min of hypothermic ...
Yamazaki Kazuhiro - - 2009
Cardioplegic arrest has been the main mechanism of myocardial protection during open-heart surgery; however, it causes myocardial injury during ischemia-reperfusion. Free radical scavengers are widely known to attenuate ischemia-reperfusion injury in various settings. We investigated the effects of edaravone, a novel free radical scavenger that was originally used for cerebral ...
O'Brien J Darcy - - 2009
BACKGROUND: Pediatric myocardium is unique from mature myocardium; thus, the use of adult cardioplegia for pediatric cardiac operations may provide suboptimal myocardial protection. We evaluated our standard adult cardioplegia (AC; modified Buckberg) and a pediatric cardioplegia (PC) solution (del Nido solution, Baxter) in vitro in rat cardiomyocytes and compared short-term ...
Fannelop Tord - - 2009
BACKGROUND: A single-dose strategy for cardioplegia is desired in minimal invasive approaches to valve surgery and aortic arch repairs. We hypothesized that a single infusion of Bretschneider HTK solution offers myocardial protection comparable to repeated cold oxygenated blood. METHODS: Sixteen pigs on bypass with 60 minutes of aortic cross-clamping were ...
Fallouh Hazem B HB Cardiac Surgical Research, The Rayne Institute (King's College London), St. Thomas' Hospital, London, UK. - - 2009
Elective temporary cardiac arrest (cardioplegia) is often required during cardiac surgery. In the 1970 s, the development of hyperkalaemic cardioplegic solutions revolutionised cardiac surgery by offering effective chemically-induced cardiac arrest and myocardial protection during global ischaemia. Despite remaining the most widely-used cardioplegic technique, hyperkalaemia can have detrimental effects due to ...
Tiryakioglu O - - 2009
The effects of double (n = 60, group 1) versus single (n = 60, group 2) aortic clamping on myocardial function and protection were investigated during coronary artery bypass grafting using a heart-lung pump. In group 1, after opening the cross clamp, proximal anastomosis was completed using side clamps and, ...
Deja Marek A - - 2009
OBJECTIVES: The study was designed to assess whether diazoxide-mediated cardioprotection might be used in human subjects during cardiac surgery. METHODS: Forty patients undergoing coronary artery bypass grafting were randomized to receive intermittent warm blood antegrade cardioplegia supplemented with either diazoxide (100 micromol/L) or placebo (n = 20 in each group). ...
Schramko Alexey A - - 2009
BACKGROUND: There is continuing concern about the effect of hydroxyethyl starch (HES) solutions on blood coagulation. Rapidly degradable HES solutions with more favorable effects on clot strength have therefore been developed. Because the risk of bleeding is increased after cardiopulmonary bypass, we examined whether these types of HES solutions could ...
Steger Christina Maria - - 2009
Isolated manifestation of sarcoidosis in the heart is very rare. The present work describes the case of a 41-year-old woman with ventricular tachycardia and severe symptoms of heart failure in June 2006. Clinical, MRI and echocardiographic findings revealed the diagnosis of an arrhythmogenic right ventricular dysplasia. Due to the severe ...
Shalaby Ahmed - - 2009
Background. We studied the effect of fast induction of cardiac arrest with denosine on myocardial bax and bcl-2 expression. Methods and Results. 40 elective CABG patients were allocated into two groups. The adenosine group (n = 20) received 250 mug/kg adenosine into the aortic root followed by blood potassium cardioplegia. ...
Verheij Gerdina - - 2009
Umbilical venous catheters (UVCs) are commonly used in the management of severely ill neonates. Several life-threatening complications have been described, including catheter-related infections, myocardial perforation, pericardial effusion and cardiac arrhythmias. This report describe two neonates with cardiac arrhythmias due to umbilical venous catheterisation. One neonate had a supraventricular tachycardia requiring ...
Köksal Hakan - - 2008
OBJECTIVE: We aimed to evaluate the efficacy of low dose N-acetylcysteine (NAC) against myocardial ischemia-reperfusion damage in coronary artery bypass surgery accompanied by cardiopulmonary bypass (CPB). METHODS: Thirty patients operated due to triple coronary artery disease were enrolled into this prospective randomized study (control group -n=15 and NAC group - ...
Kouerinis Ilias A - - 2008
"Directed cardioplegia" is a novel approach in myocardial protection in which a certain volume of cardioplegic solution is diverted into a severely diseased coronary artery after surgical occlusion of the other main branches that can be accessed by the surgeon. In this way, the surgeon is able to eliminate the ...
Sinha Pranava - - 2008
BACKGROUND: Information from clinical studies is limited regarding the optimal method for myocardial protection in immature hearts, and specifically the benefits of different cardioplegia (CP) formulations. We compared two CP techniques by evaluation of the cardiac index using thermodilution catheters. METHODS: The study cohort includes 102 neonates and infants (aged ...
Gaudino Mario - - 2008
OBJECTIVE: To evaluate a hemodynamic method for the assessment of the position of the retrograde cardioplegia catheter (RCC) versus conventional Manual Assessment. METHODS: We randomized 200 patients undergoing aortic valve surgery to Manual (n = 101) or Hemodynamic Assessment (n = 99). In the Hemodynamic group a 25% pressure increase ...
Li Gang - - 2008
BACKGROUND AND AIM OF THE STUDY: Simultaneous antegrade/retrograde cardioplegia (SARC) has been employed frequently during cardiac surgery to preserve the jeopardized myocardium. However, retrograde perfusion of SARC may interfere with myocardial drainage and disrupt myocardial fluid homeostasis, which may affect the myocardial energy metabolism and contractile function. The study was, ...
Gates Richard N - - 2008
Neonatal arch reconstructions present a challenge for myocardial protection. We report our results for eight patients treated with intermittent cold all blood retrograde cardioplegia during arch reconstruction using continuous selective normothermic cerebral perfusion. Over a 10-month period eight consecutive neonates underwent complex arch reconstruction. Mean age was 8.4 days (range ...
Rudd Donna M - - 2009
OBJECTIVE: Currently, the safe human heart preservation time is limited to around 4 to 5 hours of cold ischemic storage. Longer arrest times can lead to donor heart damage, early graft dysfunction, and chronic rejection. The aim of this study was to examine a new nondepolarizing, normokalemic preservation solution with ...
Healey Christine M - - 2009
BACKGROUND: This study examined the impact of intraoperative myocardial acidosis and adverse postoperative outcomes on the cost of cardiac surgical care. METHODS: Myocardial tissue pH corrected to 37 degrees C (pH(37C)) was measured in 162 patients with cross-clamp (XC) duration of 119 minutes or longer. Perioperative data and outcomes were ...
Radmehr Hassan - - 2008
BACKGROUND: In a prospective randomised clinical study we assessed and compared antegrade vs. combined antegrade-retrograde cardioplegia in patients who underwent elective coronary artery by pass grafting. METHODS: Between March 2006 and January 2007, 87 consecutive patients were randomly divided into two groups. Group A (n=45) received antegrade cold (4 degrees ...
Miyazawa Seiji - - 2008
Body temperature drops dramatically during hibernation, but the heart retains the ability to contract and is resistant to induction of arrhythmia. Although adaptive changes in the heart prior to hibernation may be involved in the cold-resistant property, it remains unclear whether these changes are sufficient for maintaining cardiac pulsatility under ...
Rivard Andrew L - - 2008
BACKGROUND: A major problem in procurement of donor hearts is the limited time a donor heart remains viable. After cardiectomy, ischemic hypoxia is the main cause of donor heart degradation. The global myocardial ischemia causes a cascade of oxygen radical formation that cumulates in an elevation in hydrogen ions (decrease ...
Jin Zhen-Xiao - - 2008
OBJECTIVES: We sought to evaluate a moderate-potassium cardioplegic solution using adenosine and lidocaine as the arresting and protecting cardioprotective combination in pediatric cardiac surgery. METHODS: One hundred thirty-four patients with congenital heart disease were randomly allocated to one of 3 groups according to the cardioplegia formula used: the high-potassium (HP) ...
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