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Zhang Xiaoming X Department of Neurosurgery, the Second Affiliated Hospital, Harbin Medical University, Harbin, 150086, P. R. - - 2014
For glioma, temozolomide (TMZ) is a commonly used chemotherapy drug and photodynamic therapy (PDT) is an important adjuvant therapy. The aim of this study was to evaluate the effect of their combination for the treatment of glioma. A rat C6 glioma model using male Wistar rats (n=180) weighing 280-300g was ...
Ivancic Melanie M MM Biochemistry, University of Wisconsin - - 2014
The development of non-invasive methods for early detection of colon cancer is critical for the successful management of this disease. Using a targeted quantitative proteomics technique, we assessed the ability of 12 serum proteins to detect the presence of colonic polyps in the Apc(Pirc/+)rat model of familial colon cancer. Serum ...
Tartaro Karrie K Pfizer Worldwide Research and Development, Drug Safety Research and Development , Groton, CT 06340 , - - 2014
Abstract The mononuclear phagocyte system (MPS) which provides protection against infection is made up of phagocytic cells that engulf and digest bacteria or other foreign substances. Suppression of the MPS may lead to decreased clearance of pathogenic microbes. Drug delivery systems and immunomodulatory therapeutics that target phagocytes have a potential ...
Montanari Sonia S Cell Therapy Program, Princess Margaret Hospital, University Health Network, Toronto, ON, Canada, - - 2014
The inbred rat is a suitable model for studying human disease and because of its larger size is more amenable to complex surgical manipulation than the mouse. While the rodent fulfills many of the criteria for transplantation research, an important requirement is the ability to mark and track donors cells ...
Wang Sa S Hubei Key Laboratory of Natural Products Research and Development, College of Chemistry and Life Science, China Three Gorges University, Yichang, Hubei, - - 2014
It is well known that fluorescent labeling has recently become a major research tool in molecular and cellular biology for demonstrating therapeutic mechanisms and metabolic pathways. However, few studies have reported the use of fluorescent labeling of natural products. We recently explored the boron 2-(2'-pyridyl) imidazole (BOPIM) derivative analogs, which ...
Hashimoto Hirofumi H Department of Physiology, School of Medicine, University of Occupational and Environmental Health Kitakyushu, - - 2014
Oxytocin (OXT) is well known for its ability to the milk ejection reflex and uterine contraction. It is also involved in several other behaviors, such as anti-nociception, anxiety, feeding, social recognition, and stress responses. OXT is synthesized in the magnocellular neurosecretory cells (MNCs) in the hypothalamic paraventricular (PVN) and the ...
Azzouz Inès I Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, - - 2014
The aim of the present study was to investigate the interaction of zinc chloride (3 mg/kg, intraperitoneally [ip]) in rat liver in terms of the biosynthesis of nanoparticles. Zinc treatment increased zinc content in rat liver. Analysis of fluorescence revealed the presence of red fluorescence in the liver following zinc ...
Muravyeva Maria M From the Department of Anesthesiology (M.M., I.B., M.B., Z.J.B., F.S., D.C.W., and J.R.K.), Department of Pharmacology and Toxicology (M.B., D.C.W., and J.R.K.), and Department of Physiology (Z.J.B.), Medical College of Wisconsin, Milwaukee, Wisconsin; and The Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, Wisconsin - - 2013
Diabetes alters mitochondrial bioenergetics and consequently disrupts cardioprotective signaling. The authors investigated whether mitochondrial DNA (mtDNA) modulates anesthetic preconditioning (APC) and cardiac susceptibility to ischemia-reperfusion injury by using two strains of rats, both sharing nuclear genome of type 2 diabetes mellitus (T2DN) rats and having distinct mitochondrial genomes of Wistar ...
Wang Shuo - - 2013
The majority of urinary stones have been observed to grow by circular increments in the clinic and in animal studies. However, the mechanism of stone formation has not yet been elucidated. Marking the stone at specific time-points during the growth of the stone is likely to enable the clarification of ...
Atieh Thérèse - - 2013
In severe nosocomial pneumonia, the pathogenic responsibility of bacteria isolated from airways is far from certain, and a lung biopsy is sometimes performed. However, detection and identification of pathogens are frequently unachieved. Here, we developed a protocol for direct visualization of bacteria within the lung tissue using fluorescent in situ ...
Gatzioufas Z Z Department of Ophthalmology, University Hospitals of Geneva HUG, Geneva, Switzerland. - - 2013
The purpose of the study was to investigate the presence of the uveoscleral pathway in the normotensive rat (NR) and in a rat model of congenital glaucoma (CGR). We injected the fluorescent tracer 70-kDa dextran rhodamine B in the anterior chamber of four NRs and four CGRs. At 10 and ...
Fu Qiang - - 2013
OBJECTIVE: To observe the impairing effects of triptolide on liver mitochondria in isolated rat-liver mitochondria and human normal liver HL7702 cell line. METHODS: Rat-liver mitochondria were isolated from adult female Sprague-Dawley (SD) rats. Liver mitochondria were incubated with 0, 1.25, 2.5, 5 and 10 μmol/L triptolide for detecting mitochondrial swelling ...
Trabelsi Hamdi - - 2013
The aim of this study was to investigate the interaction of cadmium chloride with mineral elements in rat nephrocytes in terms of the biosynthesis of nanocomplexes. The results show that selenium supplementation enhanced cadmium accumulation in kidneys. Analysis of the fluorescence revealed an increase in red fluorescence in the kidneys ...
Jidong W - - 2013
To evaluate the optimum dosage and time-effect relationship of lentivirus vectors mediated enhanced green fluorescence protein gene (lenti-eGFP) transfection into the rat ovary in vivo. Lenti-eGFP was microinjected into rats' ovaries with different dosage (2 x 10(6) TU and 10 x 10(6) TU virosome respectively, n = 5 rats). The ...
Sun Nannan - - 2013
The goal of biological samples' cryofixation is to trap a metabolic state as it exists in vivo by rapidly stopping internal reactions. However, obtaining perfect quality of cryofixation for large and high hypermetabolism organ/tissue (such as brain, heart) remains a challenge. The aim of this study was to develop and ...
Chen Bifeng - - 2012
A coumarin-based fluorescence chemoprobe was developed and evaluated for the selective and sensitive detection of hydrogen sulfide in degassed PBS buffers and fetal bovine serum. Fluorescence detection of hydrogen sulfide in living cells was also successfully achieved using two-photon confocal fluorescence imaging. Further in situ visualization of endogenous H(2)S was ...
Zhao Ya-Juan - - 2012
The aim of this study was to characterize the protective effects of glutamine (Gln) on brain cells undergoing experimental endotoxemia induced by lipopolysaccharide (LPS) injection. Young rats were injected with LPS or control, and a subset of LPS-injected rats were pretreated with Gln. Electron microscopy and immunohistochemistry were used to ...
Shukla Charu C Department of Biological Sciences, Kent State University, Kent, OH 44242, - - 2012
The brain melanocortin (MC) system is one of numerous overlapping systems regulating energy balance; it consists of peptides including α-melanocyte-stimulating hormone that act through melanocortin receptors (MCRs). Mutations and polymorphisms in MC3R and MC4R have been identified as one of the most common genetic contributors to obesity in human studies. ...
Lee Ho-Sung - - 2012
We for the first time report evidence for existence of novel structures, primo vessels (PVs) and primo nodes (PNs) floating inside the venous sinuses of rat brains. For this purpose, we applied a chromium-hematoxylin (Cr-Hx) solution to stain the PVs and the PNs floating inside the venous sinuses (superior sagittal ...
Fabila Diego - - 2012
A novel application of diffuse reflectance and fluorescence spectroscopy in the assessment of liver fibrosis is here reported. To induce different stages of liver fibrosis, a sufficient number of male Wistar rats were differentially exposed to chronic administration with carbon tetrachloride. Then, diffuse reflectance and fluorescence spectra were in vivo ...
Hu Essa - - 2012
A radiolabeled tracer for imaging therapeutic targets in the brain is a valuable tool for lead optimization in CNS drug discovery and for dose selection in clinical development. We report the rapid identification of a novel phosphodiesterase 10A (PDE10A) tracer candidate using a LC-MS/MS technology. This structurally distinct PDE10A tracer, ...
Ribeiro Vera Lucia Sardá - - 2012
Acetylcholinesterase (AChE), an enzyme that hydrolyses acetylcholine (ACh) at cholinergic synapses, is a target for pesticides and its inhibition by organophosphates leads to paralysis and death of arthropods. It has been demonstrated that the n-hexane extract of Calea serrata had acaricidal activity against larvae of Rhipicephalus (Boophilus) microplus and Rhipicephalus ...
Skjolding Anders Daehli - - 2012
Aims:  Aqua¬porin-4 is the most abundant cellular water channel in brain and could be a molecular basis for a cerebrospinal fluid absorption route additional to the arachnoid villi. In the search for "alternative" cerebrospinal fluid absorption pathways it is important to compare experimental findings to human pathophysiology. This study compares ...
Royal Walter W 3rd Department of Neurology, University of Maryland School of Medicine, 110 S. Paca Street, Baltimore, MD 21201, USA. - - 2012
The HIV-1 transgenic (TG) rat has been shown to be a useful model of nervous system disease that occurs in human HIV-1 infection. Studies were, therefore, performed to examine characteristics of the immune response in the periphery and brain of the animals and expression of factors in the nervous system ...
Zulli Allison L - - 2012
Optimization of a series of aminomethyl ketone diamine H(3)R antagonists to reduce the brain exposure by lowering the pKa, led to molecules with improved pharmacokinetic properties. Compounds 9, 19, and 25 had high affinity for human H(3)R and demonstrated in vivo H(3)R functional activity in the rat dipsogenia model. Compound ...
Qin Ren-An - - 2012
To observe the effects of Compound Danshen Tablets (CDST) on spatial cognition and expression of brain b-amyloid precursor protein (beta-APP) in a rat model of Alzheimer's disease. The rat model of Alzheimer's disease (AD) was established using D-galactose to cause subacute aging combined with Meynert nucleus damage. Rat behavior was ...
Hsiao Peng P Department of Pharmaceutics, University of Washington, Seattle, Washington 98195, United - - 2012
We have shown that the rat can quantitatively predict the verapamil-cyclopsorine A (CsA) drug-drug interaction (DDI) at the human blood-brain barrier (BBB). In addition, the potency (EC(50)) of CsA to inhibit rat BBB P-gp can be predicted from in vitro studies in MDRI-transfected cells. To assess if these excellent agreements ...
Ren Cheng-Tao - - 2012
The α-subunit of tetrodotoxin-resistant (TTX-R) voltage-gated sodium channel (VSGC, Nav1.5/SCN5A) has been found from the rat heart and human neuroblastoma cell line NB-1, but its expression in rat brain has not been identified radically. In this study, a reverse transcriptase-polymerase chain reaction was used to clone the full sequence of ...
Antunes Inês F - - 2012
Activation of microglia is a hallmark of inflammatory, infectious, and degenerative diseases of the central nervous system. Several studies have indicated that there is an increase in release of β-glucuronidase by activated microglia into the extracellular space at the site of neuroinflammation. β-glucuronidase is involved in the hydrolysis of glycosaminoglycans ...
Nie Binbin B Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, - - 2013
This study constructs a rat brain T2 -weighted magnetic resonance imaging template including olfactory bulb and a compatible digital atlas. The atlas contains 624 carefully delineated brain structures based on the newest (2005) edition of rat brain atlas by Paxinos and Watson. An automated procedure, as an SPM toolbox, was ...
Onoue Satomi - - 2012
The present study aimed to develop and characterize new formulations of dipyridamole (DP), a pH-dependent poorly soluble drug, employing an acidic pH-modifier for improving dissolution and absorption under hypochlorhydric condition. Granule formulations of DP (DPG) with and without fumaric acid (FA) were prepared with wet granulation, physicochemical properties of which ...
Pacheco Sara E - - 2012
Quantifying testicular homogenization-resistant spermatid heads (HRSH) is a powerful indicator of spermatogenesis. These counts have traditionally been performed manually using a hemocytometer, but this method can be time consuming and biased. We aimed to develop a protocol to reduce debris for the application of automated counting, which would allow for ...
Maebayashi Hisae - - 2012
The TRK-fused gene (TFG in human, Tfg in rat) was originally identified in human papillary thyroid cancer as a chimeric form of the NTRK1 gene. It has been reported that the gene product (TFG) plays a role in regulating phosphotyrosine-specific phosphatase-1 activity. However, no information regarding the localization of Tfg ...
Umehara Ken-Ichi - - 2011
PURPOSE: Drug elimination in the liver consists of uptake, metabolism, biliary excretion, and sinusoidal efflux from the hepatocytes to the blood. We aimed to establish an accurate prediction method for liver clearance in rats, considering these four elimination processes. In vitro assays were combined to achieve improved predictions. METHODS: In ...
Zhang Zhiwen - - 2011
Aim: A self-assembled nano-based delivery system was designed and developed to increase the oral bioavailability of poor hydrophilic and lipophilic daidzein. Methods: Daidzein was firstly combined with lecithin to form the daidzein-lecithin complex, then self-assembled into micelles (DLMs) with lecithin and sodium bile. The physiochemical properties and intestinal absorption of ...
Zhang Zhiwen - - 2012
Candesartan cilexetil (CC) is widely used for the treatment of hypertension and heart failure, but it shows very poor aqueous solubility and very low oral absorption. In this work, CC-loaded solid lipid nanoparticles (CLNs) were successfully developed to improve the oral bioavailability. The physicochemical properties of CLNs were characterized, and ...
Hickman Debra L - - 2011
Dissection of living brain tissue for in vitro experiments requires the use of a rapid euthanasia method. However, the method must not subject animals to unnecessary pain and must be aesthetically acceptable to experimenters. The purposes of the current study were to assess the aesthetics of 6 euthanasia methods, measure ...
Kadam Rajendra S RS Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, - - 2011
To determine the influence of drug lipophilicity, ocular pigmentation, and species differences on transscleral solute transport. The transport of eight β-blockers across excised sclera/sclera-choroid-RPE (SCRPE) of albino rabbit, pigmented rabbit, human, porcine, and bovine eyes was determined over 6 hours. The ex vivo transscleral β-blocker transport to the vitreous at ...
Chang Jeong-Soo - - 2011
Synthesis of new ester prodrugs of olmesartan is described. Their in vitro stabilities in simulated gastric juice, rat plasma, and rat liver microsomes were tested. And the pharmacokinetic parameters for olmesartan after their oral administration were also estimated and compared with those in case of olmesartan medoxomil. Compounds 13 and 14 ...
Linder Aurea Elizabeth AE Department of Pharmacology, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil. - - 2011
1. The serotonin transporter (SERT) handles serotonin (5-hydroxytryptamine (5-HT)) and is blocked by the antidepressant SERT inhibitors fluoxetine and fluvoxamine. Although the importance of SERT in the central nervous system is clear, SERT also functions in the peripheral vasculature. In the present study, we tested the hypothesis that the vasculature ...
Schipper Pieter - - 2011
A well-known example for gene x environment interactions in psychiatry is the one involving the low activity (s) allelic variant of the serotonin transporter (5-HTT) promoter polymorphism (5-HTTLPR) that in the context of stress increases risk for depression. In analogy, 5-HTT knockout rodents are highly responsive to early life, but ...
Possidente Mariapia - - 2011
ATP-binding cassette (ABC) proteins play key role in tissue defence by transporting metabolic waste and toxic chemicals out of the cells. Consequently, intact cell systems are required to study xenobiotic interactions with ATP-dependent transporters. The aim of the present study was to set up an intestinal precision-cut slice technique to ...
Fukami Masako - - 2011
AIM: Penicillin G is reported to increase bile flow by increasing biliary glutathione excretion, as well as the biliary excretion of penicillin G itself. In order to study the effect of penicillin G on the hepatic excretory pathway, the effect of colchicine and genipin on the increase of biliary glutathione ...
Haque Mohammed Ziaul - - 2011
NaCl reabsorption by the thick ascending limb of the loop of Henle (THAL) occurs via the apical Na-K-2Cl cotransporter, NKCC2. Overall, NKCC2 activity and NaCl reabsorption are regulated by the amount of NKCC2 at the apical surface, and also by phosphorylation. Dahl salt-sensitive rats (SS) exhibit higher NaCl reabsorption by ...
Maeng Han-Joo - - 2011
Previous studies have shown that 1α,25-dihydroxyvitamin D(3) (1,25(OH)(2) D(3) ) treatment (2.56 nmol/kg i.p. daily×4) increased PepT1, Mrp2, Mrp4, Asbt, but not Mdr1/P-gp in the rat small intestine. In this study, the intestinal everted sac technique, together with various select probes: mannitol (paracellular transport), glycylsarcosine (PepT1), 5(and 6)-carboxy-2',7'-dichlorofluorescein (CDF) diacetate ...
Maeng Han-Joo - - 2011
Previous studies have shown that 1α,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) treatment (2.56 nmol/kg i.p. daily×4) increased PepT1, Mrp2, Mrp4, Asbt, but not Mdr1/P-gp in the rat small intestine. In this study, the intestinal everted sac technique, together with various select probes: mannitol (paracellular transport), glycylsarcosine (PepT1), 5(and 6)-carboxy-2',7'-dichlorofluorescein (CDF) diacetate (precursor of ...
Wu Hongfei - - 2011
The potential for microemulsion drug delivery systems to improve the lymphatic transport and the portal absorption of a poorly water-soluble drug, puerarin, were investigated in lymph-cannulated rats. SD rats were operated for lymph duct cannulation and were orally dosed with 3ml puerarin microemulsion (0.6mg/g, n=6). The lymph and plasma were ...
Wayhs Mônica Lisboa Chang - - 2011
To evaluate the transepithelial transport of sodium, glucose, potassium, and water and the mRNA level of the sodium-glucose cotransporter (SGLT1) and the facilitated sugar transporter (GLUT2) in the small intestine of iron-deficient rats. After 6 wk of receiving diets with low or normal iron content, rats (Wistar-EPM) were subjected to ...
Parasrampuria Ridhi - - 2011
Rhodamine 123 (RH-123) and its glucuronidated metabolite (RH-Glu) are excreted into the bile via the ABC efflux transporters P-glycoprotein (P-gp) and multidrug resistance-related protein type 2 (Mrp2), respectively. In this study, we investigated the short-term (2 h) effects of a low or high concentration of nitric oxide (NO) donors sodium ...
Collins Danielle - - 2011
The process of urea nitrogen salvaging plays a vital role in the symbiotic relationship between mammals and their intestinal bacteria. The first step in this process requires the movement of urea from the mammalian bloodstream into the gastrointestinal tract lumen via specialized proteins known as facilitative urea transporters. In this ...
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