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Fretellier Nathalie N Guerbet, Research & Innovation Division, 93600 Aulnay-sous-Bois, France. Electronic address: - - 2014
This study was designed to compare the safety of two gadolinium chelates (GCs), used as contrast agents for magnetic resonance imaging, in juvenile rats. Juvenile rats received five intravenous administrations (between postnatal day [PND] 4 and 18) of gadoteric acid (macrocyclic ionic GC), gadodiamide (linear nonionic GC) or saline, and ...
Kochi Takahiro T Departments of aGastroenterology/Internal Medicine bTumor Pathology, Gifu University Graduate School of Medicine, Gifu cInstitute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, - - 2014
Establishment of an efficient rat model for colitis-associated colon carcinogenesis is critical for evaluation of the potency of cancer-preventive agents on carcinogenesis. In the present study, we examined whether the Kyoto Apc Delta (KAD) rat, a novel adenomatous polyposis coli mutant rat strain, is useful for detection of potential chemopreventive ...
Deshpande Laxmikant S LS Departments of Neurology, Virginia Commonwealth University, Richmond, Virginia 23298, - - 2014
Organophosphate (OP) compounds, including paraoxon (POX), are similar to nerve agents such as sarin. There is a growing concern that OP agents could be weaponized to cause mass civilian causalities. We have developed a rodent survival model of POX toxicity that is being used to evaluate chronic morbidity and to ...
Reichardt Holger M HM Institute for Cellular and Molecular Immunology, University of Göttingen Medical School, Humboldtallee 34, Göttingen, 37073, - - 2014
Transgenesis is a valuable tool with which to study different aspects of gene function in the context of the intact organism. During the last two decades a tremendous number of transgenic animals have been generated, and the continuous improvement of technology and the development of new systems have fostered their ...
Khalil Ashraf A AA a Department of Protein Technology , Institute of Genetic Engineering and Biotechnology, City of Scientific Research and Technological Applications , Bourg Elarab , Alexandria , - - 2014
The genus Fusarium, especially F. verticillioides and F. proliferatum have been found in several agricultural products worldwide, especially in maize. Regardless the occurrence of symptoms, the presence of Fusarium in maize constitutes an imminent risk due to its ability to produce fumonisins, mycotoxins with proven carcinogenic effect on rats, swine, ...
Remy Séverine S INSERM UMR 1064-ITUN, CHU de Nantes, Nantes F44093, France; Platform Rat Transgenesis, Nantes F44093, - - 2014
The generation of genetically modified animals is important for both research and commercial purposes. The rat is an important model organism that until recently lacked efficient genetic engineering tools. Sequence-specific nucleases, such as ZFNs, TALE nucleases, and CRISPR/Cas9 have allowed the creation of rat knockout models. Genetic engineering by homology-directed ...
Taner Gökçe G Department of Biology, Faculty of Science, Gazi University, Ankara, - - 2014
The aim of this study was to evaluate the protective effects of Pycnogenol® (Pyc), a complex plant extract from the bark of French maritime pine, on oxidative stress parameters (superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities and total glutathione (GSH) and malondialdehyde (MDA) levels), an inflammatory cytokine (tumor necrosis ...
Mezera Vojtech V a Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University in Prague, Simkova 870, 500 38 Hradec Králové 1, Czech - - 2014
Epigallocatechin gallate (EGCG) is an antioxidant found in green tea. In this study, male Wistar rats were subjected either to partial hepatectomy (PHx), or a sham operation (LAP). Twenty-four hours after surgery, hepatocytes were isolated and treated with various concentrations of EGCG for up to 72 h. We then measured ...
Occhipinti Sergio S Biotecnologie Molecolari e Scienze per la Salute, University of - - 2014
Despite the great success of HER2 vaccine strategies in animal models, effective clinical results have not yet been obtained. We studied the feasibility of using DNA coding for chimeric rat/human HER2 as a tool to break the unresponsiveness of T cells from patients with HER2-overexpressing tumors (HER2-CP) EXPERIMENTAL DESIGN: Dendritic ...
Stiborová Marie M Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech - - 2014
Aristolochic acid I (AAI) is the major toxic component of the plant extract AA, which leads to the development of nephropathy and urothelial cancer in human. Individual susceptibility to AAI-induced disease might reflect variability in enzymes that metabolise AAI. In vitro NAD(P)H:quinone oxidoreductase (NQO1) is the most potent enzyme that ...
Gubrij Igor B IB Central Arkansas Veterans Healthcare System, Medicine, Little Rock, Arkansas, United States ; - - 2014
Idiopathic pulmonary arterial hypertension (iPAH) is associated with high morbidity and mortality. We evaluated whether luminal delivery of the human prostacyclin synthase (hPGIS) cDNA with adenoassociated virus (AAV) vectors could attenuate PAH. AAV serotype 5 and 9 vectors containing the hPGIS cDNA under the control of a cytomegalovirus (CMV)-enhanced chicken ...
Ishii Yuji Y Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, - - 2014
DNA adductome analysis using liquid chromatography-tandem mass spectrometry is a promising tool to exhaustively search DNA modifications. Given that the molecular weight of chemical-specific adducts is determined by the total molecular weights of the active form and nucleotide bases, we developed a new method of comprehensive analysis for chemical-specific DNA ...
Liu Dan D Department of Pharmacology, School of Pharmacy of Chongqing Medical University, Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing - - 2014
Histone acetylation has been linked to depression, the etiology of which involves many factors such as genetics, environments, and epigenetics. The aim of the present study was to investigate whether it was associated with epigenetic histone modification and gene expression of enzymes responsible for the biosynthesis of norepinephrine and serotonin ...
Noori-Zadeh Ali A Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, - - 2014
Glial cell line-derived neurotrophic factor (GDNF) is one of the most important proteins playing a pivotal role in growing and repairing of the nervous system. GDNF therapy is one of the suggested options in the treatment of neurodegenerative diseases. Limitations in the viral gene delivery and its side effects after ...
Yang Kyunghee - - 2014
Breast cancer resistance protein (BCRP) and multidrug resistance-associated protein 2 (MRP2) are members of the ATP binding cassette (ABC) transporter family located in the canalicular membrane of hepatocytes that mediate biliary excretion of many drugs and endogenous compounds. BCRP and MRP2 have overlapping substrate profiles. Predicting drug disposition in the ...
Zhang Jihong J College of Life Science, Henan Normal University, Xinxiang 453007, Henan Province, China; Key Laboratory for Cell Differentiation Regulation, Xinxiang 453007, Henan Province, - - 2014
Interleukin 18 (IL-18) is a proinflammatory cytokine with an ability to accelerate cell proliferation through activating other factors. However, little is yet understood of the role of IL-18 in the regulation of liver regeneration (LR). To study the effect of IL-18 on LR, the gene expression profiles of hepatocytes isolated ...
Firth Cadhla C Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA - - 2014
Norway rats (Rattus norvegicus) are globally distributed and concentrate in urban environments, where they live and feed in closer proximity to human populations than most other mammals. Despite the potential role of rats as reservoirs of zoonotic diseases, the microbial diversity present in urban rat populations remains unexplored. In this ...
Kaisaki Pamela J PJ The Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford, United - - 2014
Post-translational protein modifications such as acetylation have significant regulatory roles in metabolic processes, but their relationship to both variation in gene expression and DNA sequence is unclear. We address this question in the Goto-Kakizaki (GK) rat inbred strain, a model of polygenic type 2 diabetes. Expression of the NAD-dependent deacetylase ...
Zhao Liangcai L Institute of Metabonomics & Medical NMR, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, - - 2014
Curcuma aromatica oil is a traditional herbal medicine demonstrating protective and anti-fibrosis activities in renal fibrosis patients. However, study of its mechanism of action is challenged by its multiple components and multiple targets that its active agent acts on. Nuclear magnetic resonance (NMR)-based metabonomics combined with clinical chemistry and histopathology ...
Gibson Brett B Department of Psychology, University of New Hampshire, Durham, NH 03824. Electronic address: - - 2013
Cheng and colleagues (Cheng, 1988, 1989, 1990; Cheng & Sherry, 1992; Spetch, Cheng & Mondloch, 1992) have shown that birds use vector information from landmarks to return to hidden goal locations. Cheng (1994) subsequently showed that pigeons average the distance and directional components of landmark-to-goal vectors separately, rather than as ...
Li Qiling Q Department of Obstetrics and Gynecology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, - - 2013
Gender-preferential gene expression is a widespread phenomenon in humans. It is important to study how gender differences influence the pathogenesis of various diseases and response to specific drugs. The aim of this study is to determine if the mouse albumin enhancer/promoter may serve as the promoter to introduce gender-preferential gene ...
França Lucas Martins LM Laboratory of Experimental Physiology, Department of Physiological Sciences, Federal University of Maranhão, Avenida dos Portugueses, 1966, 65.080-805, São Luís (MA), - - 2013
Non-alcoholic fatty liver disease (NAFLD) is intimately associated with insulin resistance and hypertriglyceridemia, whereas many of the mechanisms underlying this association are still poorly understood. In the present study, we investigated the relationship between microsomal triglyceride transfer protein (MTP) and markers of endoplasmic reticulum (ER) stress in the liver of ...
Kim Soo-Jeong SJ Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, - - 2013
Electroacupuncture (EA) is a modified form of acupuncture that utilizes electrical stimulation. We previously showed that EA stimulated rats were divided into responders that were sensitive to EA and non-responders that were insensitive to EA based on the tail flick latency (TFL) test. The dopamine beta-hydroxylase (DBH) gene was more ...
Ferguson Carolyn - - 2013
Genetically engineered mice are a valuable resource for studies of the behavioral effects of ethanol. However, for some behavioral tests of ethanol action, the rat is a superior model organism. Production of genetically engineered rats has been severely hampered due to technical limitations. Here we utilized a promising new technique ...
Wu Wei W Department of Pediatics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China, - - 2013
The purpose of this study was to investigate the repair of the osteoarthritis(OA)-induced cartilage injury by transfecting the new TGF-β3 fusion protein (LAP-MMP-mTGF-β3) with targeted therapy function into the bone marrow-derived mesenchymal stem cells (MSCs) in rats. The recombinant of pIRES-EGFP-MMP was constructed by combination of DNA encoding MMP enzyme ...
Wu Fei - - 2013
It is currently thought that the transforming growth factor-β (TGF-β)/Smad signaling pathway acts as a central pathway leading to liver fibrosis, and that the aberrant Wnt/β-catenin signaling pathway also plays a vital role in the development of liver fibrosis. There is evidence that the histidine triad nucleotide-binding protein 1 (Hint1) ...
Cheng Shu-Qun - - 2013
Benzo(a)pyrene (B[a]P) is an environmental carcinogen that induces tumors in many animal species, but the neurotoxic effects of B[a]P have not been well studied. In the present study, we investigated the effects of subacute exposure to B[a]P in Sprague-Dawley (SD) rats. Male rats received daily injections of either B[a]P (0, ...
Jidong Wang W Department of Obstetrics and Gynecology, First Affiliated Hospital of Chongqing medical university, Chongqing, - - 2013
To investigate the reproductive endocrine changes after inhba overexpression into rat ovary. Thirty Sprague-Dawley rats were randomly assigned to three groups. Inhba overexpression lentivirus vectors (LV-eGFP-inhba) were microinjected into rat ovary (INH group); Control animals received the same amount lentivirus vector empty (CON group) or LV-eGFP (GFP group). Antral follicle ...
Pérez-Castrillón Jose L - - 2013
BACKGROUND: The aim of this study was to evaluate gene expression of bone remodeling markers in type 2 diabetic Goto-Kakizaki (GK) nonobese rats after gastrojejunal bypass and sleeve gastroplasty and their relationship with hormonal parameters. METHODS: We designed an experimental study in three groups of GK rats (nonoperated gastrojejunal bypass ...
Wang Tao - - 2013
PURPOSE: Promoter-targeted small activating RNAs (saRNAs) have been shown to mediate sequence-specific up-regulation of gene expression. We aimed to explore the therapeutic effect of RNAa-mediated activation of inducible nitric oxide synthase (iNOS) gene on improving erectile function in a rat model of diabetes mellitus. MATERIALS AND METHODS: An optimal saRNA ...
Liu Hai Chun - - 2013
Decreased levels of serum insulin-like growth factor-1 (IGF-1) have been proven to cause osteoporosis. Gene transfer of IGF-1 offers an attractive technology to treat skeletal metabolic disorders including osteoporosis, but the viral vectors are limited by their high antigenicity and immune response. Our purpose was to investigate the expression of ...
Fukamachi Katsumi - - 2013
The recent widespread application of Cre/loxP technology has resulted in a new generation of conditional animal models that can better recapitulate many salient features of human disease. These models benefit from the ability to monitor the expression and functionality of Cre protein. We have generated a conditional (Cre/loxP dependent) LacZ ...
Bäck Susanne - - 2013
Cerebral dopamine neurotrophic factor (CDNF) protein has been shown to protect the nigrostriatal dopaminergic pathway when given as intrastriatal infusions in rat and mouse models of Parkinson's disease (PD). In this study, we assessed the neuroprotective effect of CDNF delivered with a recombinant adeno-associated viral (AAV) serotype 2 vector in ...
Nemoto Kiyomitsu - - 2013
We have previously demonstrated super-induced expression of the Grin2c gene encoding the N-methyl-D-aspartate receptor 2C subunit during the process of liver enlargement induced by phenobarbital, clofibrate, piperonyl butoxide, or lead nitrate. In the present study, hepatic Grin2c gene expression levels were assessed by real-time RT-PCR in male F344 rats fed ...
Tea Melinda - - 2013
Oxygen-induced retinopathy (OIR) is a robust animal model of human retinopathy of prematurity that readily allows changes in retinal gene and microRNA (miRNA) expression in response to fluctuations in oxygen levels to be studied. We sought to identify small non-coding RNA (ncRNA) genes that showed stable expression upon exposure to ...
Li G - - 2013
To generate rat induced pluripotent stem cells (iPSCs) using the PiggyBac (PB) transposon system, and to explore whether these iPSCs would be amenable to genetic manipulation. The PB transposon system was used to reprogramme rat embryonic fibroblasts (EF) to become iPSCs. Cells were identified with regard to pluripotency and differentiation ...
Ménoret Séverine - - 2012
Despite the recent availability of gene-specific nucleases, such as zinc-finger nucleases (ZFNs) and transcription activator-like nucleases (TALENs), there is still a need for new tools to modify the genome of different species in an efficient, rapid, and less costly manner. One aim of this study was to apply, for the ...
Zeng Yu - - 2012
Intrauterine growth retardation (IUGR) is thought to program insulin resistance, type 2 diabetes and other metabolic diseases in later life. Skeletal muscle is an important tissue involved in regulating the metabolism. We therefore hypothesized that the regulation of glucose- and lipid-related genes in skeletal muscle may contribute to metabolic changes ...
Gassner Davina - - 2012
This article presents a unique approach for the delivery of gene therapy vectors into the cochlea of the laboratory rat. Mice and guinea pigs are established in vivo models for cochlear gene therapy each of which has distinct advantages and disadvantages. The rat has some of the molecular advantages of ...
Khachatoorian Ronik R Molecular Biology Interdepartmental Ph.D. Program, Molecular Biology Institute, David Geffen School of Medicine at University of California, Los Angeles, CA, United States. - - 2013
Prior studies showed that Toll-like receptor (TLR) signaling pathway genes were upregulated in the liver of rats fed ethanol, but not in rats fed ethanol plus S-adenosylmethionine (SAMe). These results were obtained using a PCR microplate array analysis for TLRs and associated proteins such as proinflammatory cytokines and chemokine mRNA ...
Furushima Kenryo K Department of Genetics, University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, - - 2012
A hybrid piggyBac/Sleeping Beauty transposon-based insertional mutagenesis system that can be mobilized by simple breeding was established in the rat. These transposons were engineered to include gene trap sequences and a tyrosinase (Tyr) pigmentation reporter to rescue the albinism of the genetic background used in the mutagenesis strategy. Single-copy transposon ...
Schnackenberg Laura K - - 2012
Following kidney failure in domesticated pets in the US and kidney issues requiring hospitalization with some deaths in children in China, investigators determined the cause was adulteration of pet foods and baby formula with melamine. It has since been noted that exposure of rats to melamine and cyanuric acid forms ...
Zhao Liangcai - - 2012
ETHNOPHARMACOLOGICAL RELEVANCE: Zhibai Dihuang Pill (ZDP) is one of ancient traditional Chinese medicines (TCMs), which is usually used for the treatment of kidney deficiency for thousands of years in China. AIM OF THE STUDY: Traditional Chinese medicines (TCMs) usually operate in vivo through multi-components, multi-ways and multi-targets. However, the molecular ...
Koriem Khaled M M - - 2012
The kidney is one of the critical target organs for chronic cadmium toxicity. Cadmium is a cumulative nephrotoxicant, and preferentially accumulates and persists in the kidneys. The natriuretic and antidiuretic effects of methyl alcohol extracts of Chelidonium majus L. (C. majus) leaves were evaluated in kidney of cadmium-intoxicated rats. Ninety-six ...
Chang In Youb - - 2012
Calcium-binding proteins are present in the kidneys: calbindin D-28k in the distal tubules and calretinin in the proximal tubules. Since paraquat causes degeneration in the brush border-bearing proximal tubule cells in rat kidneys, we investigated the changes of calretinin immunoreactivity in the proximal tubule cells of paraquat-induced nephrotoxicity in experimental ...
Fridman Osvaldo - - 2012
Betaine-homocysteine S-methyltransferase (BHMT) is an enzyme that converts homocysteine (Hcy) to methionine using betaine as a methyl donor. Betaine also acts as osmolyte in kidney medulla, protecting cells from high extracellular osmolarity. Hepatic BHMT expression is regulated by salt intake. Hormones, particularly corticosteroids, also regulate BHMT expression in rat liver. ...
Salama Afrah F - - 2012
Thyroid hormones (THs) are essential for growth and development of the kidney. Also TH influences glomerular filtration and tubular functions. Hypothyroidism negative influences kidney function indirectly by affecting the cardiovascular system and the renal blood flow, and directly by affecting glomerular filtration, tubular functions and the structure of the kidney. ...
Meng Fanxue - - 2012
MicroRNAs (miRNAs) are small non-coding RNAs that regulate a variety of biological processes. The latest version of the miRBase database (Release 18) includes 1,157 mouse and 680 rat mature miRNAs. Only one new rat mature miRNA was added to the rat miRNA database from version 16 to version 18 of ...
Iwano Shunsuke - - 2012
Cytochrome P450(CYP)s are known to show a sexual dimorphic expression in rat livers. However, the comprehensive analysis for the sex-dependent gene expressions of drug metabolizing enzymes except for CYPs, transporters and nuclear receptors in rat livers and kidneys has not been investigated yet. The purpose of the present study was ...
Hu Cheng-Ping - - 2012
Traditional Chinese medicine suggests that renal deficiency is a causative factor of asthma, and tonifying kidney drugs are believed to be an appropriate and beneficial treatment. The adrenal medullary chromaffin cells (AMCC) transition to the neuronal phenotype is known to occur in asthma, as evidenced by degranulation of chromaffin granules, ...
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