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Results 301 - 350 of 540
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Whitelock J M - - 1991
A rat carcinoma cell line (T2/H7) constitutively synthesised interstitial collagenase. When these cells were incubated with 12-O-tetradecanoylphorbol 13-acetate (TPA) they secreted an inhibitor of collagenase, which resulted in a net decrease of collagenolytic activity being detected in conditioned medium. Using reverse zymography, the Mr of the inhibitor was found to ...
Katagiri M - - 1991
1. By means of an enzyme immunoassay, the contents of D-amino acid oxidase (DAO) were determined in kidney, liver, cerebellum and lung of hog, but the oxidase was not detectable in heart or cerebrum. 2. The oxidases in kidney, liver and cerebellum of hog were indistinguishable as regards immunoreactivity toward ...
De Jong J W - - 1991
Xanthine oxidase is the pathological form of xanthine oxidoreductase, which generates free oxygen radicals, when it converts (hypo)xanthine to urate. We studied 1. developmental changes in rat heart, 2. urate production in catheterized patients, and 3. species differences of cardiac xanthine oxidase. First, we measured the activity of the enzyme ...
Sim M K - - 1991
The activities of monoamine oxidase and phenolsulfotransferase in the hypothalamus and anterior pituitary gland of spontaneously hypertensive rats and the normotensive control (Wistar Kyoto rat) rats were investigated. The monoamine oxidase activity (determined using dopamine as substrate) in both these tissues was not significantly different between the normo- and hypertensive ...
Butler E G - - 1990
The uricosuric drug benzbromarone (3,5-dibromo-4-hydroxyphenyl)-1-(2-ethyl-3-benzofuranyl)methanone, a benzofurane derivative, was studied for its effects on parameters related to hepatic peroxisome proliferation. Groups of male F-344 rats were fed either basal diet, the peroxisome proliferator clofibrate at 5000 ppm as a comparison compound, or benzbromarone at two doses, 1000 and 2000 ppm. ...
Singh A K - - 1990
1. The molecular composition of acetylcholinesterase (AChE) obtained from cockroach neural, and rat brain tissues was different. Vertebrate enzyme exhibited a higher degree of polymerization than insect enzyme. 2. Acephate was a potent inhibitor of cockroach AChE, but a poor inhibitor of rat AChE. Unlike acephate, methamidophos was a potent ...
Li F - - 1990
The activities of coproporphyrinogen oxidase, protoporphyrinogen oxidase and ferrochelatase in liver homogenates of iron-overloaded and acutely or chronically ethanol-treated rats were assayed by high performance liquid chromatographic methods. Decreased coproporphyrinogen oxidase activity was found in chronically carbonyliron-fed rats while the activities of protoporphyrinogen oxidase and ferrochelatase remained unchanged. Coproporphyrinogen oxidase ...
Klockgether T - - 1990
Systemically administered N-methyl-D-aspartate (NMDA) antagonists, MK-801 ((+)5-methyl-10,11-dihydro-5H-dibenzo(a,d)cyclohepten-5,10-imine maleate) and CPP (3-[(+-)-2-carboxypiperazin-4-yl]-propyl-1-phosphonate), potentiate the ability of L-dopa (L-3,4-dihydroxyphenylalanine) to reverse akinesia and to alleviate muscular rigidity in monoamine-depleted rats. On the basis of these findings, it is proposed that NMDA antagonists may be beneficial as adjunctive treatment in the therapy of ...
Obata T - - 1990
An endogenous inhibitor of monoamine oxidase (MAO) was separated by gel-filtration from 105,000 g supernate of T4-treated rat liver cytosol. The inhibition by this inhibitor was concentration-dependent and more potent for A-form MAO than for B-form MAO. The mode of inhibition was competitive either with 5-hydroxytryptamine or beta-phenylethylamine. The molecular ...
Curti D - - 1990
The apparent Km for cytochrome c of cytochrome oxidase does not change but the Vmax decreases in synaptosomes and non-synaptic mitochondria isolated from the cerebral cortex as a whole of 30-month-old rats compared with 4-month-old ones. When the subcellular organelles are submitted to stressful conditions, namely incubation in media of ...
Azevedo I - - 1990
Rat vasa deferentia were incubated with 0.2 mumol/l 3H-noradrenaline for 60 min and then washed out with amine-free solution for 100 min. Autoradiography then revealed a preferential labelling of the varicosities in the immediate vicinity of the surface of the tissue. However, when tissues were obtained from reserpine- and pargyline-pretreated ...
Molineaux C J - - 1990
Conversion of the octapeptide dynorphin (Dyn) A-(1-8) to Leu5-enkephalin (LE) by endopeptidase EC 3.4.24.15 (EP-24.15) in vivo was examined using the technique of ventriculocisternal perfusion. Peptides were administered intracerebroventricularly in the presence or absence of the EP-24.15 inhibitor N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-Ala-Phe-p-aminobenzoate (cFPAAF-pAB) via cannulae placed into the lateral ventricle of urethane-anesthetized rats. ...
Yano S - - 1990
Isoelectric variants of Cu,Zn-superoxide dismutase (Cu,Zn-SOD) have been reported to exist in various organs including rat liver. To elucidate the biochemical characteristics of the variants, rat liver Cu,Zn-SOD was purified and isolated into eight variants, i.e., pI 5.15, 4.88, 4.80, 4.75, 4.70, 4.65, 4.60, and 4.50. The pI 4.88 variant ...
Mizobe T - - 1990
The alteration of monoamines and their metabolites in the brain during and after hemorrhagic shock in the conscious state was measured in rats. Blood pressure was maintained at 40-70 mmHg (5.3-9.3 kPa) for 60 min by withdrawing 8 ml of blood intermittently. The content of monoamines, as well as their ...
Obata T - - 1990
T4-induced endogenous monoamine oxidase (MAO) inhibitor in rat liver perfusate by perfusion with sucrose buffer and lipid peroxide (LPO) and superoxide dismutase (SOD) in rat liver tissues were investigated after pretreatment with total hepatic ischemia. When the gel filtration of these rats liver perfusate were carried out using Sephadex G-25, ...
Varalakshmi P - - 1990
The effect of oral administration of Crataeva nurvala bark decoction on calcium oxalate lithiasis has been studied in rats. The elevation of the oxalate-synthesizing liver enzyme, glycolate oxidase, produced by feeding glycollic acid was remarkably reduced with the decoction, showing a regulatory action on endogenous oxalate synthesis. Protein-bound carbohydrates were ...
Naiman N - - 1990
Previous reports indicate that 4-benzyl-1-methyl-1,2,3,6-tetrahydropyridine (BMTP), the benzyl analogue of the Parkinsonian inducing neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), is not neurotoxic in the C-57 black mouse even when administered at a dose 10 times greater than the dose of MPTP required to cause an 85% depletion of neostriatal dopamine. Intrastriatal microdialysis in ...
Wakasaki H - - 1990
The synthesis of lysyl oxidase, which initiates the cross-linking of collagen and elastin, was investigated in carbon tetrachloride (CCl4) induced fibrotic liver of rat. Lysyl oxidase activity of the fibrotic liver was 4 times greater than that of normal liver. mRNAs from the livers of normal and CCl4-treated rats were ...
Obata T - - 1990
1. After selective binding of [3H]pargyline to either monoamine oxidase (MAO) A or MAO B in the rat liver, MAO B alone in the rat brain and MAO in carp brain and liver, molecular weight and isoelectric points (pI) of these MAO were determined by sodium dodecyl sulphate (SDS)-polyacrylamide gel ...
Colombo M - - 1990
Milacemide was found to protect Swiss albino CD1 mice but not Sprague Dawley rats against bicuculline-induced lethality. Since it had been previously suggested that the anticonvulsant activity of milacemide might be related to MAO-B- mediated glycine formation, brain and liver MAO-A and-B activities and the urinary metabolic pattern of milacemide ...
Fogel W A - - 1990
The distribution of mucosal monoamine and polyamine oxidases along rat gastrointestinal tract presents a complementary pattern to that of diamine oxidase. The maximal diamine oxidase values are found in the last two thirds of small intestine, whereas the highest activities of monoamine and polyamine oxidase occur in the proximal duodenum ...
Nowak J Z JZ Department of Biogenic Amines, Polish Academy of Sciences, - - 1990
Accumulation or inhibition of cAMP formation in response to dopamine or dopamine related drugs in the absence or in the presence of forskolin and/or IBMX was investigated in isolated rat retina. While the existence of D1-receptors (positively coupled with adenylate cyclase) was confirmed, D2-receptors (negatively coupled to adenylate cyclase) were ...
Borbe H O - - 1990
The monoamine oxidase (MAO) B activity of rat brain was inhibited by selegiline and its desmethyl-metabolite in vitro with IC50-values of 11.25 nmol/l and 625.00 nmol/l, respectively. When measured in an ex vivo experiment following oral treatment of rats, the large difference in potency was distinctly reduced, from factor 60 ...
Yu P H - - 1990
2-Propyl-1-aminopentane (2-PAP) and N-(2-propylpentyl)glycinamide (PPG) were readily deaminated by rat liver monoamine oxidase B and rat aorta semicarbazide-sensitive amine oxidase. The deaminated product, valproic acid (VPA), was identified by HPLC-fluorometric assessment. Absorption and biotransformation of these compounds and their VPA metabolite into the brain were rapid processes. An investigation was ...
Clow A - - 1989
Isatin has recently been identified in rat tissues and normal human urine where it constitutes the major part of the endogenous monoamine oxidase inhibitor, tribulin. In this study, we have measured both isatin (by gas chromatography/mass spectrometry) and tribulin (inhibition of a standard rat liver monoamine oxidase preparation) in extracts ...
Obata T - - 1989
Amine oxidase activity in plasma of rats were investigated after pretreatment with the perilobular hepatotoxin allyl formate (AF). Amine oxidase activities in plasma elevated after administration of AF 0.1 ml/kg i.p. to male rats with 1 microM and 100 microM benzylamine (Bz), 10 microM beta-phenylethylamine (beta-PEA) and 100 microM serotonin ...
Obata T - - 1989
The effect of diethylnitrosamine (DEN), a well known experimental carcinogen, MAO-A and MAO-B activity of rat liver was investigated. The oxidations of both 5-HT (MAO-A) and beta-PEA (MAO-B) were inhibited by DEN in vitro. DEN is reversible inhibitor, after enzyme-inhibitor preincubation. MAO-B activity in rat liver was decreased by continuous ...
Francis A J - - 1989
Abnormalities of the neural suture were observed in cultured rat embryos exposed to oxygen radicals generated by xanthine and xanthine oxidase. The distribution of the severity of these abnormalities was altered by the addition of L-ascorbic acid (AA) or DL-alpha-tocopherol (AT). The antioxidant effect of AA and AT were probably ...
Minakami H - - 1989
Thirty liver specimens from 30 pre-eclamptic women with and without liver dysfunction were examined for the amount of lipofuscin pigment deposited. The amount of hepatic lipofuscin correlated positively with plasma urate concentrations. This finding may indicate an involvement of oxygen-free radicals and xanthine oxidase in the pathophysiology of pre-eclampsia.
Efange S M - - 1989
The neurotoxic metabolite of N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), 1-methyl-4-phenylpyridinium, selectively accumulates in dopaminergic neurons via the dopamine reuptake system. Consequently, nontoxic radiolabeled MPTP analogs may be potentially useful for visualizing catecholaminergic neurons in vivo. N-Methyl-4-(4-hydroxy-3-[125I]iodobenzyl)-1,2,3,6-tetrahydropyridine [( 125I]MHTP), an analog of the nontoxic N-methyl-4-benzyl-1,2,3,6-tetrahydropyridine, has been studied in rats and mice. After intravenous ...
Isel F - - 1989
Three closely related aminopyridazine derivatives: minaprine [3-(2-morpholino-ethylamino)-4-methyl-6-phenyl pyridazine, dihydrochloride], CM 30366 [3-(2-morpholino-ethylamino)-4-methyl-6-(4-hydroxyphenyl) pyridazine, hydrobromide] and SR 95191 [3-(2-morpholino-ethylamino)-4-cyano-6-phenyl pyridazine] were examined for their inhibitory effects on mouse-killing behavior (MKB). Three groups of killer rats were used: spontaneous killer rats (K rats) and nonkillers which became killers following para-chlorophenylalanine (PCPA) treatment ...
Barwell C J - - 1989
The selectivity of the naturally occurring amine, N,N-dimethyltyramine (hordenine) for monoamine oxidase (MAO) and its action upon isolated vasa deferentia of the rat was investigated. Hordenine was deaminated by rat liver MAO with a Michaelis constant of 479 microM and maximum velocity of 128 nmol (mg protein)-1 h-1 compared with ...
Booth R G - - 1989
The in vivo dopaminergic neurotoxicity of a series of quinoline and isoquinoline derivatives was assessed in rats using an intrastriatal microdialysis technique that measures the release of dopamine. The N-methyl quaternary salts of these two heterocyclic aromatic systems displayed about 10% of the potency of MPP+ in this assay. Furthermore, ...
Murakami Y - - 1989
Antizyme inhibitor was highly purified from rat liver by using affinity chromatography. It has some structural resemblance to ornithine decarboxylase (ODC), as judged from Mr, immunoreactivity and reversible binding with antizyme. However, unlike hepatic amounts of ODC and ODC-antizyme complex, that of antizyme inhibitor did not show much fluctuation upon ...
Mennigen R - - 1989
We have suggested previously that the histamine-diamine oxidase system is involved in cell proliferation. Therefore, the diamine oxidase activity and the histamine content were studied during mucosal proliferation induced by 70% resection of the small intestine of the rat with and without aminoguanidine (AG), a specific inhibitor of diamine oxidase ...
Joseph X - - 1989
Isoproterenol produces myocardial necrosis in rats. To investigate the possible role of oxygen free radicals generated by xanthine oxidase and neutrophils, we examined the effects of the xanthine oxidase inhibitors, 6-mercaptopurine (6MP) and 6-thioguanine (6TG) combined and allopurinol, or of irradiation (to induce leukopenia) on isoproterenol-induced myocardial necrosis (ISOMN). The ...
Nishikimi M - - 1989
A mutant strain of Wistar rats with L-gulono-gamma-lactone oxidase deficiency has recently been established. To investigate this deficiency by DNA and RNA blot hybridization analyses, a fragment of a previously cloned cDNA encoding rat L-gulono-gamma-lactone oxidase was used as a probe. When genomic DNA of the mutant rat was digested ...
Masson-Pévet M - - 1989
In the mammalian pineal gland, serotonin (5-HT) is located both in the pinealocytes and in the noradrenergic nerve terminals. Pineal 5-HT can be metabolized by three different routes, one of these being its deamination, catalyzed by monoamine oxidase (MAO). MAO is known to exist as two isozymes, MAO-A and MAO-B. ...
Obata T - - 1989
The effect of diethylnitrosamine (DEN), a well-known experimental carcinogen, toward MAO-A and MAO-B activity of rat liver was investigated. The oxidations of both beta-PEA (MAO-B) and 5-HT (MAO-A) were inhibited by DEN. The K1 values of DEN in the inhibition of rat liver MAO-A and MAO-B activity were determined. The ...
Truong D D - - 1989
Milacemide is a glycine prodrug with reported antiepileptic antimyoclonic properties. In this study, milacemide increased "wet dog shakes" in rats pretreated with 5-Hydroxytryptophan (5-HTP) and carbidopa. Moreover, it worsened the serotonin behavior syndrome precipitated by 5-HTP and the monoamine oxidase inhibitor tranylcypromine. The serotonin syndrome was also elicited by the ...
Stancheva S - - 1989
1. The effect of the nootropic drug adafenoxate on monoamine oxidase (MAO) activity in rat brain cortex, striatum, hypothalamus and hippocampus has been studied using the following substrates: tyramine (total MAO), serotonin (MAO A) and beta-phenylethylamine (MAO B). 2. In a series of increased concentrations (from 5 x 10(-4) up ...
Nomura Y - - 1989
Several biochemical parameters reflecting the degree of senescence were compared between senile-prone (P) and resistant (R) strains of male senescence-accelerated mouse (SAM) at 11 to 12 months of age. Plasma testosterone in SAM-P amounted to half the concentration in SAM-R. In the liver and brain of SAM-P, the content of ...
Krupinski K - - 1989
The antithrombotic effects of three thrombin inhibitors (hirudin, NAPAP and argidipine) were investigated in an experimental thrombosis model using laser lesions of rat mesenteric venules. Furthermore, their in vitro anticoagulant activity (partial thromboplastin time, thrombin time, Heptest, inhibition of factor IIa and factor Xa) in human platelet poor plasma (PPP) ...
Smith D F DF Aarhus University Institute of Psychiatry, Psychiatric Hospital, Risskov, - - 1989
The effects of lithium on monoamine oxidase (MAO) in rat brain were studied in the presence of each enantiomer of tranylcypromine [(+)- and (-)-TCP]. The (+)-enantiomer was 25 times more potent than its antipode as MAO inhibitor. Lithium enhanced the inhibitory effect of (-)-TCP on MAO, but it did not ...
Da Prada M - - 1989
Moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (MAO) inhibitors. In vitro (rat brain homogenates) moclobemide inhibits MAO-A selectively with lower potency than many of the reference MAO inhibitors. However, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference ...
Dyck L E - - 1989
The basal and 50 mM K+-stimulated release of m-tyramine (mTA), p-tyramine (pTA), tryptamine (TR) and phenylethylamine (PE) from striatal slices obtained from rats pretreated with a monoamine oxidase inhibitor (MAOI) was investigated. A K+-stimulated release of mTA and pTA was observed, but K+ did not stimulate either TR or PE ...
Reid A A - - 1988
The effect of tricyclic antidepressant drugs on the deamination of phenylethylamine and benzylamine by monoamine oxidase (MAO) type B was investigated in vitro in human brain cortex, human platelet, and rat brain preparations. These drugs inhibited MAO activity as expected; however, an atypical biphasic response was observed with the tertiary ...
Villalba R M - - 1988
The indirect immunofluorescence technique to detect endogenous C-flanking peptide of neuropeptide Y (NPY), C-PON, in combination with NADPH-diaphorase (NADPH-d) histochemistry, were applied to the same sections to establish whether C-PON containing cell bodies in the rat striatum can be labelled by their content of NADPH-diaphorase activity. NADPH-diaphorase activity proved to ...
Grohmann M - - 1988
The neuronal disposition of the side chain-labelled 3H-7-(-)noradrenaline and the ring-labelled 3H-2,5,6-(-)noradrenaline of relatively high specific activity was compared in the (incubated) rabbit aorta, the extraneuronal one in the (perfused) rat heart. Furthermore, the metabolism by monoamine oxidase (MAO) and catechol-O-methyl transferase (COMT) of the labelled amines was studied in ...
Willoughby J - - 1988
This study describes the histochemical localisation of monoamine oxidase (MAO) in marmoset brain. No MAO A staining was observed but MAO B was found in many areas, with medium intensity of staining in the substantia nigra, and high intensity staining in the striatum and nucleus accumbens, among other regions. The ...
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