The role of nitric oxide in the mechanical repression of RANKL in bone stromal cells/Kemigin stromal hucrelerinde RANKL uzerinde nitrik oksideri etkisi.
Abstract: Mechanical loading and nitric oxide (NO) have positive influences on bone mass. NO is induced by strain via upregulation of eNOS mRNA and protein. Strain causes decreased RANKL. Primary Stromal cells from wild-type (WT) and eNOS(-/-) mice showed strain inhibition of RANKL expression was prevented by NOS inhibitors (L-NAME and LNMMA) in WT Stromal cells. Stromal cells from eNOS(-/-) mice showed mechanical repression of RANKL expression (p<0.05). Mechanical strain still increased NO production in the absence of eNOS, and was abolished by SMTC, a specific nNOS inhibitor. nNOS mRNA and protein expression were increased by strain in eNOS(-/-) but not in WT cells, revealing that nNOS was mechanically sensitive. When NO synthesis was blocked with either SMTC or siRNA targeting nNOS in eNOS(-/-) cells however, strain still was able to suppress RANKL expression by 34%. Strain suppression of RANKL can occur through non-NO dependent pathways.
Article Type: Author abstract
Subject: Osteoporosis (Development and progression)
Nitric oxide (Physiological aspects)
Bones (Density)
Bones (Health aspects)
Authors: Rahnert, J.
Fan, X.
Case, N.
Murphy T.C.
Grassi, F.
Sen, B.
Rubin, J.
Pub Date: 08/01/2008
Publication: Name: From the Osteoporosis World Publisher: Galenos Yayincilik Audience: Academic Format: Magazine/Journal Subject: Health Copyright: COPYRIGHT 2008 Galenos Yayincilik ISSN: 1300-9141
Issue: Date: August, 2008
Product: Product Code: 2813772 Nitric Oxide NAICS Code: 32512 Industrial Gas Manufacturing SIC Code: 2813 Industrial gases
Accession Number: 195755866
Full Text: Rahnert J, Fan X, Case N, Murphy TC, Grassi F, Sen B, Rubin J

Bone 2008,43:48-54
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