West Nile Virus neurologic disease in humans, South Africa, September 2008-May 2009.
Subject: West Nile fever (Development and progression)
West Nile fever (Diagnosis)
Infection (Diagnosis)
Infection (Development and progression)
Nervous system diseases (Diagnosis)
Nervous system diseases (Development and progression)
Immunoglobulins
Authors: Zaayman, Dewald
Venter, Marietjie
Pub Date: 12/01/2012
Publication: Name: Emerging Infectious Diseases Publisher: U.S. National Center for Infectious Diseases Audience: Academic; Professional Format: Magazine/Journal Subject: Health Copyright: COPYRIGHT 2012 U.S. National Center for Infectious Diseases ISSN: 1080-6040
Issue: Date: Dec, 2012 Source Volume: 18 Source Issue: 12
Product: SIC Code: 2836 Biological products exc. diagnostic
Geographic: Geographic Scope: South Africa Geographic Code: 6SOUT South Africa
Accession Number: 313345661
Full Text: West Nile virus (WNV), a mosquito-borne flavivirus (1), is a reemerging pathogen of global concern (2). Febrile illness occurs in -20% of WNV-infected persons; neurologic complications (e.g., meningitis, encephalitis, flaccid paralysis) occur in <1% (3).

Detection of IgM in serum or cerebrospinal fluid (CSF) is the preferred method for diagnosing WNV infection; however, because of cross-reactivity between flaviviruses, positive results should be confirmed by virus neutralization assay. Early WNV infection can be diagnosed by PCR and virus isolation (4), but success has been limited in diagnosing more advanced disease with these techniques.

WNV is endemic to southern Africa. In 1947, one of the largest WNV epidemics recorded occurred in the Karoo region of South Africa (5), and another occurred in combination with a Sindbis virus epidemic in 1983-84 in the Witwatersrand-Pretoria region of South Africa. The most recent seroprevalence data for WNV in the Pretoria region of South Africa is from the 1970s (reviewed in [6]). To update that information, we determined whether WNV is being overlooked as a possible cause of disease in persons hospitalized in South Africa. The University of Pretoria Research Ethics Committee approved this study.

The Study

Serum and CSF samples were obtained from the National Health Laboratory Service, Thswane Academic Division, Thswane, South Africa, which serves public sector hospitals in northern South Africa. To select samples for testing, we reviewed laboratory submission requests for patients with clinical conditions consistent with WNV infection: fever, headache, rash, or neurologic signs (7,8). A total of 206 patient samples (15 CSF and 191 serum) were selected. During September 2008-May 2009, we screened samples for the presence of WNV by using real-time reverse transcription PCR (rRT-PCR) (9), virus neutralization assay, and IgM ELISA.

We detected WNV neutralizing antibodies in serum and CSF samples by using a modified method (10). In brief, we mixed 50% tissue culture infective doses of Kunjin virus strain MRM61C (100 U/mL) in 2% fetal bovine serum (Invitrogen, Carlsbad, CA, USA) with 2-fold dilutions of heat-inactivated patient serum (1:10-1:640) in equal volumes and incubated the mixture for 1 h at 37[degrees]C in 5% CO2. We then added 1 volume of Vero cells (1 x 105 cells/mL) in 2% fetal bovine serum, 100 U/mL of penicillin, and 100 Lig/mL of streptomycin (Lonza, Basel, Switzerland) and incubated the mixture for 72 h at 37[degrees]C in 5% CO2. Samples were considered positive for WNV neutralizing antibodies if <25% of the cells/well displayed cytopathic effect. Comparative testing with Wesselsbron virus, a closely related flavivirus, did not show cross-reactivity within the parameters of what we considered positive.

Of the 206 specimens, 40 (19.42%, 95% CI 14.02%-24.82%) were positive for neutralizing antibodies. Of these, 36/191 serum samples had antibody titers of <160. The positive CSF samples (4/15) had antibody titers of 4 (Table 1). Positive serum samples were also tested by WNV IgM capture ELISA (Panbio; Alere, Sinnamon Park, QLD, Australia); 2 had positive results (Table 1).

Of the 206 specimens, 190 were of sufficient quantity to be subjected to RNA extraction (QIAamp Viral RNA Mini Kit; QIAGEN, Valencia, CA, USA) and subsequent WNV nested rRT-PCR (9). The presence of lineage 2 WNV RNA was identified in 1 CSF specimen and confirmed by sequencing (GenBank accession no. JX974605) (Figure).

To evaluate the sensitivity of these molecular and serologic tests for diagnosing WNV in humans, we used the same methods to test 9 archived sequential serum samples in parallel (Table 2). The samples were from a patient with WNV encephalitis who became infected with neuroinvasive lineage 2 WNV strain in 2003 after a needlestick injury (11). Samples were collected 0-30 days after exposure. Initial symptoms developed on postexposure day 7 and persisted for 19 days; the patient completely recovered by day 26 (11).

Conclusions

We conducted a retrospective investigation of patients hospitalized with febrile illness or neurologic disease of unknown etiology in the Pretoria region of South Africa to determine whether some of the cases could be ascribed to WNV infection. Evidence of acute WNV infection was identified in samples for 7 patients (Table 1). For 2 of the patients, WNV infection was identified by the presence of IgM and neutralizing antibodies in serum samples; these patients had been hospitalized for febrile illness. For the other 5 patients, infection was identified by a WNV-positive (by PCR) CSF sample (1 patient) and by the presence of neutralizing antibodies in CSF samples (4 patients); these patients had been hospitalized for neurologic signs and symptoms.

The 4 patients with neutralizing antibodies in CSF all had severe neurologic complications (Table 1). Samples from these patients were insufficient for performing IgM testing; thus, WNV infection cannot be definitively determined. However, the presence of WNV neutralizing antibody in CSF samples plus acute clinical signs and symptoms of WNV infection provide a high index of suspicion for WNV infection in these patients. Factors such as increased blood-brain barrier permeability and the persistence of WNV antibodies long after infection may also serve as explanations for the presence of neutralizing antibodies in their CSF.

Nested rRT-PCR results and phylogenetic analysis confirmed the presence of lineage 2 WNV in the CSF sample from 1 patient (Figure); sequencing showed that the virus is closely related to 2 neuroinvasive WNV lineage 2 strains identified in South Africa (11,12) (Table 1). The low rate of PCR-positive cases was not entirely unexpected and may be explained by 2 factors: 1) PCR has limited success for detecting arboviruses because CSF contains low levels of virus and arbovirus-associated viremia is brief (13), and 2) false-negative test results may occur if samples are not properly stored to protect the integrity of potential viral RNA.

[FIGURE OMITTED]

To evaluate the sensitivity of the 3 diagnostic methods used in our study, we conducted a time-trial experiment by using retrospective serum samples from a patient in whom WNV meningoencephalitis developed after a needlestick injury (11). Early samples (postexposure days 8 and 9) were positive for WNV by rRT-PCR only. Results for samples obtained [greater than or equal to] 13 and [greater than or equal to] 16 days after exposure were positive by neutralization assays and IgM ELISA, respectively (Table 2). Experiments with horses have indicated that WNV neutralizing antibody assays show a positive result earlier than IgM ELISAs; the reasons for this are undetermined (14). Although our time-trial experiment reflects findings from only 1 patient and should ideally be performed on a cohort, the results, considered with those from the studies in horses (14), may imply that some cases of WNV infection in humans and animals may be missed if IgM ELISA is the only serologic test used.

Using virus neutralization assays, we identified the presence of WNV antibodies in 36/204 serum samples from patients with febrile and neurologic illness in South Africa. This finding indicates that the patients were exposed to WNV. In addition, results were negative for the patients in our study who were tested for herpes simplex virus types 1 and 2, measles, mumps, and enteroviruses, and no other etiologic agent was found. Thus, infection with WNV should be included in the differential diagnosis of patients in this region with neurologic disease, especially considering the frequent detection of severe neurologic disease in horses in the region (15).

Our findings confirm that WNV is being overlooked as a cause of severe neurologic disease in South Africa, and they suggest a need for increased clinical awareness, enhanced prospective surveillance, and a more current serosurvey of WNV infection in humans. PCR may be a useful diagnostic method during early infection, but after seroconversion has taken place, serologic tests (e.g., IgM ELISA in conjunction with virus neutralization) are more likely to yield accurate results.

Acknowledgment

We gratefully acknowledge Marianne Wolfaardt for technical assistance provided during optimization of the West Nile virus neutralization test.

This study was funded by the National Research Foundation and the National Health Laboratory Services.

Mr Zaayman is pursuing a PhD degree in the Zoonosis Unit, Department of Medical Virology, University of Pretoria. He is conducting research on the development and application of molecular and immunological tools for the differential diagnosis of West Nile virus in South Africa.

Prof Venter is head of the Zoonoses Research Unit in the Department of Medical Virology, University of Pretoria, Pretoria, South Africa, and head of the Center of Respiratory Diseases and Meningitis, National Institute for Communicable Diseases, Sandringham, South Africa. Her primary research interests are arboviruses and their association with neurological diseases in humans and animals in Africa and viral causes of severe acute respiratory infections.

Author affiliations: University of Pretoria, Pretoria, South Africa (D. Zaayman, M. Venter); and National Institute for Communicable Diseases, Johannesburg, South Africa (M. Venter)

DOI: 10.3201/eid1812.111208

References

(1.) Asnis DS, Conetta R, Teixeira AA, Waldman G, Sampson BA. The West Nile virus outbreak of 1999 in New York: the Flushing Hospital experience. Clin Infect Dis. 2000;30:413-8. http://dx.doi. org/10.1086/313737

(2.) Lanciotti RS, Roehrig JT, Deubel V, Smith J, Parker M, Steele K, et al. Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States. Science. 1999;286:2333-7. http://dx.doi.org/10.1126/science.286.5448.2333

(3.) Kulasekera VL, Kramer L, Nasci RS, Mostashari F, Cherry B, Trock SC, et al. West Nile virus infection in mosquitoes, birds, horses, and humans, Staten Island, New York, 2000. Emerg Infect Dis. 2001;7:722-5.

(4.) Sampathkumar P. West Nile virus: epidemiology, clinical presentation, diagnosis, and prevention. Mayo Clin Proc. 2003;78:1137-44. http://dx.doi.org/10.4065/78.9.1137

(5.) McIntosh BM, Jupp PG. Epidemics of West Nile and Sindbis viruses in South Africa with Culex (Culex) univittatus Theobald as vector. S Afr J Sci. 1976;72:295-300.

(6.) Jupp PG. The ecology of West Nile virus in South Africa and the occurrence of outbreaks in humans. Ann N Y Acad Sci. 2001;951:14352. http://dx.doi.org/10.1111/j.1749-6632.2001.tb02692.x

(7.) Hayes EB, Sejvar JJ, Zaki SR, Lanciotti RS, Bode AV, Campbell GL. Virology, pathology, and clinical manifestations of West Nile virus disease. Emerg Infect Dis. 2005;11:1174-9. http://dx.doi. org/10.3201/eid1108.050289b

(8.) Iwamoto M, Jernigan DB, Guasch A, Trepka MJ, Blackmore CG, Hellinger WC, et al. Transmission of West Nile virus from an organ donor to four transplant recipients. N Engl J Med. 2003;348:2196203. http://dx.doi.org/10.1056/NEJMoa022987

(9.) Zaayman D, Human S, Venter M. A highly sensitive method for the detection and genotyping of West Nile virus by real-time PCR. J Virol Methods. 2009;157:155-60. http://dx.doi.org/10.1016/jjviromet. 2008.12.014

(10.) Grandien M, Forsgren M, Ehrnst A. Enteroviruses and reoviruses. 6th ed. Washington (DC): American Public Health Association; 1989.

(11.) Venter M, Burt FJ, Blumberg L, Fickl H, Paweska J, Swanepoel R. Cytokine induction after laboratory-acquired West Nile virus infection. N Engl J Med. 2009;360:1260-2. http://dx.doi.org/10.1056/ NEJMc0808647

(12.) Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular Evolutionary genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28:2731-9. http://dx.doi.org/10.1093/ molbev/msr121

(13.) Lanciotti RS, Kerst AJ, Nasci RS, Godsey MS, Mitchell CJ, Savage HM, et al. Rapid detection of West Nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay. J Clin Microbiol. 2000;38:4066-71.

(14.) Castillo-Olivares J, Mansfield KL, Phipps LP, Johnson N, Tearle J, Fooks AR. Antibody response in horses following experimental infection with West Nile virus lineages 1 and 2. Transbound Emerg Dis. 2011;58:206-12. http://dx.doi.org/10.1111/j.1865-1682. 2010.01197.x

(15.) Venter M, Swanepoel R. West Nile virus lineage 2 as a cause of zoonotic neurological disease in humans and horses in southern Africa. Vector Borne Zoonotic Dis. 2010;10:659-64. http://dx.doi. org/10.1089/vbz.2009.0230

Address for correspondence: Marietjie Venter, Zoonoses Research Unit, Department Medical Virology, Pathology Bldg, Room 2.72, Faculty of Health Science, 5 Bophela Rd, University of Pretoria, South Africa, 2000; email: marietjiev@nicd.ac.za

The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the Centers for Disease Control and Prevention or the institutions with which the authors are affiliated.
Table 1. Characteristics and clinical information for 7
acutely ill patients with WNV infection, South Africa *

           Date sample
Sample     collected
no.

4562       2008 Nov

6208       2008 Nov

8785       2009 Jan

3111       2009 Feb
0269       2009 Apr

0312       2009 Apr

SAH 5238   2008 Oct

           Patient age,
Sample     y/sex
no.

4562       45/M

6208       35/F

8785       36/M

3111       5/M
0269       11/M

0312       26/M

SAH 5238   2/M

           Sample
Sample     type
no.

4562       CSF

6208       CSF

8785       CSF

3111       CSF
0269       Serum

0312       Serum

SAH 5238   CSF

           WNV antigen/antibody test
Sample     AN (titer)   IgM   PCR
no.

4562       Pos (4)      --    --

6208       Pos (4)      --    --

8785       Pos (4)      --    --

3111       Pos (4)      --    --
0269       Pos (40)     Pos   Neg

0312       Pos (80)     Pos   Neg

SAH 5238   Neg          Neg   Pos

Sample     Clinical information
no.

4562       Suspected HIV encephalopathy or PML, TPHA
           neg, HIV neg, paraparesis
6208       Hepatomegaly, lymphadenopathy, fever,
           vomiting, epigastric pain, EBV IgM neg, EBV
           IgG pos, malaria neg, hepatitis neg
8785       Acute paresis of lower limbs, delirium, HSV-1
           and HSV-2 neg (PCR), HTLV-1 neg, TPHA neg
3111       Meningitis, enterovirus pos (PCR)
0269       Rash, fever, Brucella neg (PCR),
           coxsackieviruses B1-B6 neg, cMv IgM neg,
           CMV IgG pos
0312       Severe headache, fever, suspected
           enterovirus, coxsackieviruses B1-B5 neg,
           Rickettsia conorii neg, EBV IgM neg
SAH 5238   Decreased level of consciousness, rash, fever,
           meningitis, measles neg, mumps neg

* WNV, West Nile virus; AN, antibody neutralization; CSF,
cerebrospinal fluid; pos, positive; -, insufficient sample for
testing; PML, progressive multifocal leukoencephalopathy; TPHA,
Treponema pallidum hemagglutination assay; neg, negative; EBV,
Epstein-Barr virus; HSV-1 and -2, herpes simplex virus
types 1 and 2; HTLV, human T-lymphotropic virus; CMV,
cytomegalovirus.

Table 2. Results of a time-trial experiment with serum
samples from a WNV-infected person, South Africa *

Days after
exposure           IgM          Neutralization
to WNV       ELISA ([dagger])   assay (titer)
0                  Neg               Neg
8                  Neg               Neg
9                  Neg               Neg
10                 Neg               Neg
11                 Neg               Neg
13                 Neg             Pos (20)
16                 Pos             Pos (40)
26                 Pos             Pos (40)
30                 Pos             Pos (80)

Days after
exposure
to WNV          Nested PCR
0                  Neg
8                  Pos
9                  Pos
10                 Neg
11                 Neg
13                 Neg
16                 Neg
26                 Neg
30                 Neg
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