Prevalence and molecular characterization of Cyclospora cayetanensis, Henan, China.
Subject: Medical research (Health aspects)
Medical research (Chemical properties)
Medicine, Experimental (Health aspects)
Medicine, Experimental (Chemical properties)
Genes (Health aspects)
Genes (Chemical properties)
RNA (Health aspects)
RNA (Chemical properties)
Prevalence studies (Epidemiology) (Health aspects)
Prevalence studies (Epidemiology) (Chemical properties)
Waterborne infections (Care and treatment)
Waterborne infections (Health aspects)
Waterborne infections (Chemical properties)
Authors: Zhou, Yang
Lv, Biao
Wang, Qiang
Wang, Rongjun
Jian, Fuchun
Zhang, Longxian
Ning, Changshen
Fu, Kanda
Wang, Yaqiang
Qi, Meng
Yao, Huixia
Zhao, Jinfeng
Zhang, Xiaoshan
Sun, Yanru
Shi, Ke
Arrowood, Michael J.
Xiao, Lihua
Pub Date: 10/01/2011
Publication: Name: Emerging Infectious Diseases Publisher: U.S. National Center for Infectious Diseases Audience: Academic; Professional Format: Magazine/Journal Subject: Health Copyright: COPYRIGHT 2011 U.S. National Center for Infectious Diseases ISSN: 1080-6040
Issue: Date: Oct, 2011 Source Volume: 17 Source Issue: 10
Product: Product Code: 8000200 Medical Research; 9105220 Health Research Programs; 8000240 Epilepsy & Muscle Disease R&D NAICS Code: 54171 Research and Development in the Physical, Engineering, and Life Sciences; 92312 Administration of Public Health Programs
Geographic: Geographic Scope: China Geographic Code: 9CHIN China
Accession Number: 269431914
Full Text: Cyclospora cayetanensis, a protozoan that causes watery diarrhea, fatigue, abdominal pain, weight loss, and inappetence, is endemic to some nonindustrialized countries (1-4). In industrialized countries, the infection has been traditionally associated with diarrheal illness in travelers to disease-endemic regions. However, since the 1990s, many foodborne and several waterborne outbreaks have been reported in North America (2,3).

Henan is an agricultural province in central China with a population of >100 million. To better understand the prevalence of cyclosporiasis and genetically characterize C. cayetanensis, we conducted a 23-month investigation of cyclosporiasis in patients treated at hospitals in the province.

The Study

The study was conducted in 2 urban areas, Zhengzhou and Kaifeng. A total of 11,554 (6,939 male; 4,615 female) child and adult patients at 3 hospitals (Huai River Hospital and 155th Liberation Army Hospital, Kaifeng, and Number One People's Hospital, Zhengzhou) were enrolled in this study during June 2007-December 2008 and JulyOctober 2009. Only data concerning age, sex, and diarrhea presence or absence were made available to laboratorians. One stool specimen from each patient was examined for Cyclospora spp. by microscopy of fecal materials that were concentrated by the formalin-ethyl acetate sedimentation method and stained with the modified acid-fast staining technique (1). We used the [chi square] test to compare the frequency of Cyclospora spp. infection among patients according to age group and sex and by season of the year. Differences were considered significant if p<0.05.

Cyclospora oocysts were detected in 81 (0.70%; 95% confidence interval [CI] 0.70% [+ or -] 0.15%) of 11,554 patients by microscopy (Table 1). Oocysts were variably stained from light pink to deep purple or remained unstained (Figure 1, panel A). They measured 8.61 [+ or -] 0.32 x 8.64 [+ or -] 0.33 um, with a length/width shape index of 1.01 (n = 55; Figure 1, panel B), and showed typical blue autofluorescence under an epifluorescence microscope with a 330-380 nm excitation filter (Figure 1, panel C). Oocysts sporulated at 32[degrees]C <13 days in 2.5% potassium dichromate.

Cyclospora oocysts were seen in samples from patients in all age groups, although the age group 7-17 years had the highest detection rate (1.47%, 95% CI [+ or -] 0.91%; Pearson correlation >0.05) (Table 1). No significant difference was found in detection rate by patient sex; the infection rates for female and male patients were 0.74% (34/4,615, 95% CI [+ or -] 0.25%) and 0.68% (47/6,939, 95% CI [+ or -] 0.19%), respectively (Pearson correlation >0.05). The overall infection rate of C. cayetanensis was similar between Zhengzhou and Kaifeng: 0.60% (95% CI 0.60 [+ or -] 0.19%) versus 0.79% (95% CI [+ or -] 0.22%) (Pearson correlation >0.05; Table 1).

The prevalence of cyclosporiasis was markedly seasonal, occurring only during July through November, with a sharp peak in August (Table 2). The occurrence of cyclosporiasis coincided with the rainy season and lagged slightly behind the peaks for mean temperature and precipitation in the year (Figure 2).

Among patients in this investigation, 5,533 had records documenting presence or absence of diarrhea at the time of specimen submission. The detection rate of Cyclospora oocysts was significantly higher for patients with diarrhea (2.97% or 12/404; 95% CI 2.97 [+ or -] 0.52%) than for patients without diarrhea (0.66% or 34/5,129; 95% CI 0.66 [+ or -] 0.22%) (Pearson correlation <0.01).

Genomic DNA was extracted from Cyclospora oocysts from 35 randomly chosen patients; the oocysts were purified by sucrose gradient centrifugation by using the Mag Extractor-Genome kit (Toyobo Co. Ltd, Osaka, Japan). A nested PCR was used to amplify a 501-bp fragment of the 18S rRNA gene (5). All 35 microscopy-positive specimens produced the expected PCR product and were sequenced successfully. The C. cayetanensis identity was established by comparing the sequences obtained with a full sequence (AF111183) of the 18S rRNA gene of C. cayetanensis from Guatemala and 3 partial sequences (AB368541, AB368542, and AB368543) from Japan. In addition, this comparison revealed the presence of 2 polymorphic sites at nucleotide positions 687 and 696 of the full gene, with a few other inconsistent nucleotide substitutions at other positions. Thus, 3 isolates had a C to T substitution at position 687, and 5 isolates plus AB368542 and AB368543 had a C to T substitution at position 696. Nevertheless, similarities among the 35 C. cayetanensis isolates and reference sequences were 99.6%-100% at the 18S rRNA locus. Representative sequences of the partial 18S rRNA gene generated were deposited in the GenBank database under accession nos. GQ292774-GQ292782, FJ009120 FJ009129, and EU860998-EU861002.

[FIGURE 1 OMITTED]

Conclusions

The overall infection rate of 0.70% (95% CI [+ or -] 0.15%) in this 23-month investigation in 2 Henan Province cities is similar to data previously obtained in an urban area in the neighboring Anhui Province (0.92%, 95% CI [+ or -] 1.04%) (6) and studies in Albania (0.63%, 95% CI [+ or -] 0.55%) (7) and Tanzania (0.91, 95% CI [+ or -] 1.25%) (8) but higher than the infection rate in the United Kingdom (0.07%, 95% CI [+ or -] 0.07%) (9). The rate is significantly lower than those in surveys conducted in healthy populations in other countries (3,10). The fact that C. cayetanensis does not always cause clinical symptoms might have contributed to the differences in infection rates among studies (3). In addition, C. cayetanensis is mainly spread by consumption of contaminated fresh produce and water (2,3). In China, persons are less likely to eat raw vegetables and drink unboiled tap water, which are known sources of sporulated C. cayetanensis oocysts in nonindustrialized countries (3). As expected, in this study the C. cayetanensis detection rate was higher for patients with diarrhea than in those without diarrhea because cyclosporiasis has been associated with mild-to-moderate self-limiting diarrhea in children and protracted and severe diarrhea in HIV-positive adults (2,3,11).

In addition to differences in study populations, socioeconomic conditions, and cultural habits, local climatic factors may have contributed to the variation in prevalence of cyclosporiasis observed in different studies. In this study, transmission of C. cayetanensis was seasonal; of the 81 cases of cyclosporiasis detected, 95% (95% CI 95% [+ or -] 4.7%) occurred during July 1-September 30, the hottest and rainiest months of the year. This result differs from the peak transmission of C. cayetanensis in some other geographic areas. For example, in Lima, Peru, C. cayetanensis prevalence peaks in warm months (December-May) in the absence of rain (1). In contrast, in Haiti where ambient temperature is high year-round, C. cayetanensis infection coincides with the cooler part of the year (10).

Thus far, 19 species of Cyclospora spp. have been described (3), but only 4 of them, C. cayetanensis and 3 species from nonhuman primates, have been characterized by sequence analysis of the 18S, 5.8S, and 28S rRNA genes and the associated internal transcribed spacer (3,12). Currently, no reliable genotyping or subtyping tools are available for the investigation of C. cayetanensis transmission, the only known Cyclospora species that infects humans (3). In our study, detection of C. cayetanensis in human stool specimens was confirmed by DNA sequencing of the partial 18S rRNA gene. We identified 2 polymorphic sites in the partial 18S rRNA gene of C. cayetanensis, although the meaning of the sequence polymorphism remains unclear.

[FIGURE 2 OMITTED]

In conclusion, C. cayetanensis infects humans in Henan Province at a relatively low frequency but with a marked seasonality. Additional research is needed to determine disease effects, transmission routes, and risk factors for C. cayetanensis infection in humans in Henan and elsewhere in China. Research could be facilitated by development of genotyping and subtyping tools for the differentiation and tracking of C. cayetanensis isolates.

This study was supported in part by Henan Province Major Research Fund of Public Welfare (no. 81100912300), the National Natural Science Foundation of China (nos. 30871863 and 30928019), and the Key National Science and Technology Special Projects (no. 2008ZX10004-011).

Dr Zhou is an assistant researcher at Henan Agricultural University. His research interests focus on the molecular epidemiology of enteric pathogens, primarily Cyclospora spp., Cryptosporidium spp., and Giardia spp.

References

(1.) Madico G, Mcdonald J, Gilman RH, Cabrera L, Sterling CR. Epidemiology and treatment of Cyclospora cayetanensis infection in Peruvian children. Clin Infect Dis. 1997;24:977-81. doi:10.1093/clinids/24.5.977

(2.) Shields JM, Olson BH. Cyclospora cayetanensis: a review of an emerging parasitic coccidian. Int J Parasitol. 2003;33:371-91. doi:10.1016/S0020-7519(02)00268-0

(3.) Ortega YR, Sanchez R. Update on Cyclospora cayetanensis, a foodborne and waterborne parasite. Clin Microbiol Rev. 2010;23:218 34. doi:10.1128/CMR.00026-09

(4.) Sterling CR, Ortega YR. Cyclospora: an enigma worth unraveling. Emerg Infect Dis. 1999;5:48-53. doi:10.3201/eid0501.990106

(5.) Li G, Xiao S, Zhou R, Li W, Wadeh H. Molecular characterization of Cyclospora-like organism from dairy cattle. Parasitol Res. 2007;100:955-61. doi:10.1007/s00436-006-0380-z

(6.) Wang KX, Li CP, Wang J, Tian Y. Cyclospora cayetanensis in Anhui, China. World J Gastroenterol. 2002;8:1144-8.

(7.) Jelinek T, Lotze M, Eichenlaub S, Loscher T, Nothdurft HD. Prevalence of infection with Cryptosporidium parvum and Cyclospora cayetanensis among international travellers. Gut. 1997;41:801-4. doi:10.1136/gut.41.6.801

(8.) Cegielski JP, Ortega YR, McKee S, Madden JF, Gaido LL, Schwartz DA, et al. Cryptosporidium, Enterocytozoon, and Cyclospora infections in pediatric and adult patients with diarrhea in Tanzania. Clin Infect Dis. 1999;28:314-21. doi:10.1086/515131

(9.) Clarke SC, McIntyre M. The incidence of Cyclospora cayetanensis in stool samples submitted to a district hospital. Epidemiol Infect. 1996;117:189-93. doi:10.1017/S0950268800001308

(10.) Eberhard ML, Nace EK, Freeman AR, Streit TG, da Silva AJ, Lammie PJ. Cyclospora cayetanensis infections in Haiti: a common occurrence in the absence of watery diarrhea. Am J Trop Med Hyg. 1999;60:584-6.

(11.) Pape JW, Verdier RI, Boncy M, Boncy J, Johnson WD Jr. Cyclospora infection in adults infected with HIV: clinical manifestations, treatment, and prophylaxis. Ann Intern Med. 1994;121:654-7.

(12.) Eberhard ML, da Silva AJ, Lilley BG, Pieniazek NJ. Morphologic and molecular characterization of new Cyclospora species from Ethiopian monkeys: C. cercopitheci sp.n., C. colobi sp.n., and C. papionis sp.n. Emerg Infect Dis. 1999;5:651-8. doi:10.3201/eid0505.990505

Yang Zhou, [1] Biao Lv, [1] Qiang Wang, Rongjun Wang, Fuchun Jian, Longxian Zhang, Changshen Ning, Kanda Fu, Yaqiang Wang, Meng Qi, Huixia Yao, Jinfeng Zhao, Xiaoshan Zhang, Yanru Sun, Ke Shi, Michael J. Arrowood, and Lihua Xiao

[1] These authors contributed equally to this article.

College of Animal Science and Veterinary Medicine-Henan Agricultural University, Zhengzhou, People's Republic of China (Y. Zhou, B. Lv, Q. Wang, R. Wang, F. Jian, L. Zhang, C. Ning, M. Qi, H. Yao, J. Zhao, X. Zhang, Y. Sun, K. Shi); Henan University Huaihe Hospital, Kaifeng, People's Republic of China (K. Fu, Y. Wang); and Centers for Disease Control and Prevention, Atlanta, Georgia, USA (M.J. Arrowood, L. Xiao)

DOI: 10.3201/eid1710.101296

Address for correspondence: Longxian Zhang, College of Animal Science and Veterinary Medicine-Henan Agricultural University, Zhengzhou 450002, People's Republic of China; email: zhanglx8999@yahoo.com.cn
Table 1. Prevalence and distribution of Cyclospora cayetanensis by
patient age, sex, and residential area, Henan Province, China,
2009-2010 *

                                No. infected/       Detection rate
Variable                         no. patients         (95% CI), %

Sex ([dagger])
  M                                47/6,939      0.68 ([+ or -] 0.01)
  F                                34/4,615      0.74 ([+ or -] 0.25)
Age, y
  [less than or equal to] 6         6/926        0.65 ([+ or -] 0.52)
  7-17                              10/678       1.47 ([+ or -] 0.91)
  18-28                            13/1,301      1.00 ([+ or -] 0.17)
  29-44                            15/2,343      0.64 ([+ or -] 0.32)
  [greater than or equal           37/6,306      0.59 ([+ or -] 0.19)
    to] 45
Areat
  Kaifeng                          48/6,093      0.79 ([+ or -] 0.22)
  Zhengzhou                        33/5,461      0.60 ([+ or -] 0.20)
Total                             81/11,554      0.70 ([+ or -] 0.15)

* CI, confidence interval.

([dagger]) Pearson correlation >0.05.

Table 2. Monthly prevalence of Cyclospora cayetanensis in
Kaifeng and Zhengzhou, Henan Province, China, 2009-2010 *

                                  No. infected/      Infection rate
Month                             no. patients        (95% CI), %

Jan                                   0/123                0
Feb                                   0/89                 0
Mar                                   0/595                0
Apr                                   0/905                0
May                                   0/388                0
Jun                                   0/410                0
Jul ([dagger])                      21/1,1814     1.16 ([+ or -] 0.49)
Aug ([double dagger])               44/2,529      1.74 ([+ or -] 0.51)
Sep ([dagger])([double dagger])     12/1,860      0.65 ([+ or -] 0.37)
Oct ([dagger])([double dagger])      3/1,638      0.18 ([+ or -] 0.21)
Nov ([dagger])([double dagger])       1/740       0.14 ([+ or -] 0.27)
Dec                                   0/463                0

* CI, confidence interval.

([dagger]) Pearson correlation <0.05.

([double dagger]) Pearson correlation <0.01.
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