New rural focus of plague, Algeria.
Article Type: Letter to the editor
Subject: Yersinia pestis (Health aspects)
Yersinia pestis (Research)
Plague (Causes of)
Plague (Research)
Authors: Bitam, Idir
Ayyadurai, Saravanan
Kernif, Tartar
Chetta, Mohammed
Boulaghman, Nabil
Raoult, Didier
Drancourt, Michel
Pub Date: 10/01/2010
Publication: Name: Emerging Infectious Diseases Publisher: U.S. National Center for Infectious Diseases Audience: Academic; Professional Format: Magazine/Journal Subject: Health Copyright: COPYRIGHT 2010 U.S. National Center for Infectious Diseases ISSN: 1080-6040
Issue: Date: Oct, 2010 Source Volume: 16 Source Issue: 10
Topic: Event Code: 310 Science & research
Geographic: Geographic Scope: Algeria Geographic Code: 6ALGE Algeria
Accession Number: 238895957
Full Text: To the Editor: Plague is a deadly rodent-associated flea-borne zoonosis caused by the bacterium Yersinia pestis (1). Human plague periodically reemerges in so-called plague foci, as illustrated by the 2003 reemergence of human plague in the Oran area, Algeria (2,3). We report emergence of a new plague focus in a remote region of Algeria.

In July 2008, three patients came to Laghouat University Hospital with signs of severe infection and painful, inflamed, enlarged lymph nodes suggestive of buboes. One additional patient became ill with pneumonia and coma after a bubo appeared. The patients were nomads living in a 24-person camp in Thait El Maa in the Laghouat area, 550 km southwest of Algiers (Figure). Plague was confirmed by culturing Y. pestis from 1 bubo aspirate. Ten days of oral doxycycline (4 mg/kg/d) combined with oral rifampin (20 mg/kg/d) and intramuscular gentamicin (3 mg/kg/d) cured the patients with bubonic plague, but the patient with pneumonic plague died.

In January 2009, eight individuals of the rodent species Meriones shawii (Shaw's jird) and 2 Psamommys obesus (fat sand rats) were trapped inside nomads' tents (H.P. Sherman Traps, Tallahassee, FL, USA). At time of capture, there was a cold wind with blowing sand, and, after visual inspection of the rodents, efforts to recover fleas failed. DNA from the rodents' spleens was extracted by using the QIAamp Tissue Kit (QIAGEN, Hilden, Germany) at the Medical Entomology Unit Laboratory, Pasteur Institute, Algiers, and subjected to PCR amplification of the plasminogen activator gene (pla) from 6 M. shawii jirds. Negative controls (DNA extracted from uninfected fleas maintained as colonies in Medical Entomology Unit Laboratory was used in the absence of negative animal tissue) remained negative.


After sequencing, the PCR amplicons showed 100% sequence identity with Y. pestis reference sequences. Identification was further confirmed in Marseille, France, by culturing 2 rodent glycerol-negative Y. pestis isolates (Algeria 1 and Algeria 2) and sequencing pla, caf, and glpD genes. The latter sequence was identical to the reference Y. pestis CO92, an Orientalis biotype. Multispacer sequence typing found the following combination: spacer Yp3, type 5; Yp4, 1; Yp5, 1; Yp7, 8; Yp8, 2; Yp9, 2; and Yp10, 1, a pattern that is typical for all Orientalis isolates investigated by this method but does not match the combinations observed for other genotypes. The original spacer Yp7 type 8 ruled out contamination (4).

National health records indicate that plague foci have been known for decades in Algiers, Kahelia, Aumale, Philippeville, and Oran, where plague reemerged in 2003 after its abence for >50 years (2,3). In the Oran outbreak, it was not clear if reemergence resulted from importation through the international port of Oran or from a previously unknown rural focus (3). The Laghouat area was not previously known as a plague focus, and plague must therefore be regarded as an emerging disease in this region.

No patients with plague reported handling sick animals. Thus, the patients likely acquired plague from rodent flea bites. Because human ectoparasites were not found on the nomads, rodent ectoparasites must have transmitted the disease. It is unlikely plague had been imported into this region; the 2 rodent species from which Y. pestis was recovered are present in the area. To the best of the nomads' knowledge, there have been no reports of movements of commensal rats or other plague-susceptible rodents into the area near the sites where the patients acquired their illnesses.

We found Y. pestis in M. shawii jirds, a native rodent species living in close contact with human populations. M. shawii jirds have been shown to be a plague-resistant species (5) and thus are an efficient reservoir for Y. pestis. These data verify the presence of a new, rural zoonotic focus of plague. This situation is worrisome because nomads remain in close contact with rodents and fleas and the risk of further outbreaks remains high. In Oran and Laghouat, an Orientalis biotype sharing the same Yp8 and Yp9 spacer sequences was found, but limited multiple spacer typing of Oran strains hampered further comparisons beyond the biotype level (3).

A plague focus has been recently detected in a neighboring Libyan focus located at the same latitude as the Laghouat area (6). Our report suggests that extending surveillance to adjacent Libya and Mauritania, which also have natural foci of plague, is necessary. The reasons for emergence of plague in these regions are unknown, but Y. pestis can survive in the soil under laboratory conditions, possibly providing the opportunity for rodents to be infected and promoting reemergence of the disease (7,8).

Emergence of plague in an area of Algeria where it had never been reported illustrates the necessity to reinforce surveillance of plague in possible rodent hosts and their ectoparasites, which are in contact with humans, to prevent emergence and reemergence of this deadly infection. Surveillance should be maintained to monitor this natural focus and potential spread of plague that might occur because of climatic or habitat influences (9).

DOI: 10.3201/eid1610.091854


We thank the Direction de la Prevention, Ministere de la Sante Algerienne, and Mohammed Chaki for technical assistance.

This study was supported by the Ministere de la Sante Algerienne and Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes.

Idir Bitam, Saravanan Ayyadurai, Tartar Kernif, Mohammed Chetta, Nabil Boulaghman, Didier Raoult, and Michel Drancourt

Author affiliations: Institut Pasteur d'Algerie, Hamma, Algeria (I. Bitam, T. Kernif); Universite de la Mediterranee, Marseille, France (S. Ayyadurai, D. Raoult, M. Drancourt); and Hopital Universitaire de Laghouat, Laghouat, Algeria (M. Chetta, N. Boulaghman)


(1.) Gage KL, Kosoy MY. Natural history of plague: perspectives from more than a century of research. Annu Rev Entomol. 2005;50:505-28. DOI: 10.1146/annurev. ento.50.071803.130337

(2.) Bertherat E, Bekhoucha S, Chougrani S, Razik F, Duchemin JB, Houti L, et al. Plague reappearance in Algeria after 50 years, 2003. Emerg Infect Dis. 2007;13:1459-62.

(3.) Bitam I, Baziz B, Rolain JM, Belkaid M, Raoult D. Zoonotic focus of plague, Algeria. Emerg Infect Dis. 2006;12:1975-7.

(4.) Drancourt M, Roux V, Dang LV, TranHung L, Castex D, Chenal-Francisque V, et al. Genotyping, Orientalis-like Yersinia pestis, and plague pandemics. Emerg Infect Dis. 2004;10:1585-92.

(5.) Baltazard M, Bahmanyar M, Mofidi C, Seydian B. Le foyer de peste du Kurdistan. Bulletin de l'Organisation Mondiale de la Sante 1952;52:441-72.

(6.) Tarantola A, Mollet T, Gueguen J, Barboza P, Bertherat E. Plague outbreak in the Libyan Arab Jamahiriya. Eurosurveillance. 2009 Jul 2;14(26)pii: 19258.

(7.) Eisen RJ, Petersen JM, Higgins CL, Wong D, Levy CE, Mead PS, et al. Persistence of Yersinia pestis in soil under natural conditions. Emerg Infect Dis. 2008;14:941-3. DOI: 10.3201/eid1406.080029

(8.) Ayyadurai S, Houhamdi L, Lepidi H, Nappez C, Raoult D, Drancourt M. Long-term persistence of virulent Yersinia pestis in soil. Microbiology. 2008;154:2865-71. DOI: 10.1099/mic.0.2007/016154-0

(9.) Duplantier JM, Duchemin JB, Chanteau S, Carniel E. From the recent lessons of the Malagasy foci towards a global understanding of the factors involved in plague reemergence. Vet Res. 2005;36:437-53. DOI: 10.1051/vetres:2005007

Address for correspondence: Michel Drancourt, Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Faculte de Medecine, 27 blvd Jean Moulin, 13385 Marseille, France; email:
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