New host records for Caliothrips striatus (thysanoptera: thripidae) on Magnolia spp. with the description of the second instar.
Article Type: Report
Subject: Entomology (Research)
Thrips (Distribution)
Liriodendron tulipifera (Diseases and pests)
Animal feeding behavior (Analysis)
Authors: Tyler-Julian, Kara
Demirozer, Ozan
Figlar, Richard
Skarlinsky, Thomas
Funderburk, Joe
Knox, Gary
Pub Date: 06/01/2012
Publication: Name: Florida Entomologist Publisher: Florida Entomological Society Audience: Academic Format: Magazine/Journal Subject: Biological sciences Copyright: COPYRIGHT 2012 Florida Entomological Society ISSN: 0015-4040
Issue: Date: June, 2012 Source Volume: 95 Source Issue: 2
Topic: Event Code: 310 Science & research; 690 Goods & services distribution Advertising Code: 59 Channels of Distribution Canadian Subject Form: Animal feeding behaviour Computer Subject: Company distribution practices
Geographic: Geographic Scope: United States Geographic Code: 1USA United States
Accession Number: 299988830
Full Text: In the current literature, apart from brief descriptions of feeding hosts, little is known regarding the habits of Caliothrips striatus Hood. This species is native to North America with a range of the eastern United States; and Wilson (1975) described it as mainly using tulip trees, Liriodendron tulipifera L., as a host plant and only occasionally being found on magnolias.

The host association information for C. striatus is limited. The feeding behavior is known only on L. tulipifera, and the plant hosts utilized for reproduction have not been documented. The current study addresses this issue.

[FIGURE 1 OMITTED]

In the present study observations were conducted at arboretums in South Carolina (N 34[degrees] 53' 52.22", W 82[degrees] 49' 43.90", 315 m asl), Georgia (N. 34[degrees] 16' 08.61", 83[degrees] 26' 58.82", 222 m asl) and Tennessee (N 35[degrees] 52' 21.85", W 88[degrees] 50' 40.08", 125 m asl) from Sep to Nov in 2010 and 2011. In the arboretums the thrips were found in large numbers causing severe feeding damage to the leaves (Fig. 1). Damage caused by C. striatus on species of Magnolia appeared as yellow-brown flecks covering the leaf surface, and sometimes the adults were visible on the leaves appearing black with 2 white bars. The adults inhabited the upper sides of the leaves except when temperatures dropped below 4[degrees] C at which point they took refuge on the undersides of the leaves.

Samplings were made from Aug to Nov 2011 at 2 locations in Quincy, Gadsden County, Florida: North Florida Research and Education Center (N 30[degrees] 32' 54.1", W 84[degrees] 35 '74.5", 71 m asl) and Lake Talquin State Forest, Bear Creek

Tract (N 30[degrees] 28' 58.6", W 084[degrees] 37' 39.6", 45 m asl). The strike method (Steiner 1962) was used to sample the thrips on Magnolia grandiflora L. and L. tulipifera. Three branches were sampled at heights ranging from 30 cm to 4 m on 5 L. tulipifera trees and 13 M. grandiflora trees weekly from 8-VIII-2011 to 25-X-2011 for a total of 11 sample dates. These thrips were placed into vials containing 70% ethyl alcohol. Thrips also were collected by visual examination of the leaves and captured with a fine brush. Adults and larvae were mounted on slides in Hoyer's and Canada balsam media, respectively. The collected adults were identified from (Wilson 1975), and compared with type specimens at the United States National Museum. In order to ascertain a positive identity of the larvae of C. striatus, which has not been previously described, live adult individuals were reared in a growth chamber (26 [+ or -] 1 [degrees]C, 50% RH) and fed with M. grandiflora leaves. This study provides the first known description of the second instar of C. striatus.

In the study 9 new host associations for C. striatus were confirmed. In the South Carolina arboretum C. striatus was found on: Magnolia delavayi Franch, Magnolia foveolata (Merr. ex Dandy) Figlar, Magnolia grandiflora L., Magnolia laevifolia (Y. W. Law & Y. F. Wu) Noot., Magnolia lotungensis Chun & C. H. Tsoong, Magnolia maudiae (Dunn) Figlar, Magnolia tamaulipana Vazquez, Magnolia virginiana L. and M. insignis x M. grandiflora (hybrid). The thrips were found in large numbers at the arboretums in Tennessee and Georgia on M. grandiflora.

In Quincy, Florida, more C. striatus were found on M. grandiflora (36 adults [28[female], 8[male]], 8 larvae) than on L. tulipifera (3 [female] adults, 0 larvae). A supplementary collection method utilizing Winkler traps (Belshaw & Bolton 1994; Sabu et al. 2011) was used in order to determine the presence of C. striatus in the leaf litter. Five different points were sampled on 5 dates from 29-IX-2011 to 7-XI2011 for a total of 25 samples. At each site 0.15 m2 of leaf litter was collected from 4 different locations underneath the canopy. One sample yielded 5 (4[female], 1[male]) adult individuals among the leaf litter below M. grandiflora and L. tulipifera trees on 6-X-2011.

At the arboretum in South Carolina a drought caused L. tulipifera to lose the leaves earlier than usual during late summer and early fall of 2010 and 2011. This may have caused C. striatus to shift to other hosts such as Magnolia due to environmental conditions. In Florida, L. tulipifera and M. grandiflora kept their foliage throughout Sep and Oct of 2011. Populations of C. striatus reproduced on M. grandiflora, but no larvae were found on L. tulipifera. During the study more adults were found on M. grandiflora. The infestation of C. striatus on numerous species of Magnolia in the arboretums in the southeastern United States raises concern about potential damage for nursery owners and growers.

Below is the second instar description of Caliothrips striatus Hood. The chaetotaxy and other morphological designations in this description are the same used in descriptions of the second instars of other Caliothrips spp. in Vierbergen et al. (2010).

Body, 1.07 mm length, color creamy white, with a continuous crimson marginal pigmentation that extends from the apex of prothorax to posterior margin of tergite VIII (Fig. 2A). Legs and antenna shaded gray. Antenna 7-segmented with the final segment, nearly 10 x longer than its basal width. Most dorsal setae clavate with their bases set on tubercles. Ventral setae apically pointed. Mesothorax, metathorax and abdominal tergites I-VII with transverse rows of longitudinally elongate dermal plaques without microtrichia. Thoracic sternites and abdominal sternite I with randomly oriented minute plaques and sternites II-IX with transverse rows of minute plaques.

Head with setae D3 pointed; all others clavate. The D1 setae are nearly half the length of the D2 setae. The D4 and D2 are similar in length (Fig. 2B). Pronotal setae D4 pointed and D6 bluntly pointed with the other 5 pairs clavate. Discal integument with small oval randomly oriented plaques. Mesonotal setae D3 blunt, D6 minute and the other 5 pairs clavate with the D2 being the shortest. The derm between the D1 and D2 setae composed of uniform transverse rows of longitudinally elongate plaques with each row being alternated by a row of smaller plaques (Fig. 2C). The rows of smaller plaques are oriented in a disorganized array between and posterior of the D1 setae. Metanotal setae D2 pointed, with the setae of the other 3 pairs clavate, integument similar to mesonotum.

Tergite I D1 setae minute, D2 clavate similar in length to the D2 seta of the other abdominal segments. Tergites II-VIII, each with 3 pairs of clavate setae. Abdominal segments IX and X each with 3 pairs of thin, apically pointed setae. The medial integument of tergites II-VIII with alternating transverse rows of plaques of 2 different sizes, similar to the mesonotum, but the rows of smaller plaques are present after every 3 consec utive rows of larger plaques. This character state is most evident on tergites II-VI (Fig. 2D). Tergite IX with 2 to 3 transverse rows of closely set minute plaques and the posterior margin lacks sclerotized teeth.

[FIGURE 2 OMITTED]

Summary

Previous reports of Caliothrips striatus Hood implied that the species is mainly found on L. tulipifera and only occasionally on Magnolia species. Results from the current study clarify the known hosts of C. striatus which include L. tu lipifera and 9 Magnolia spp. The discovery of numerous adults and larvae on the leaves showed that M. grandiflora is a host used for feeding and reproduction by C. striatus.

References Cited

Belshaw, R., and Bolton, B. 1994. A survey of the leaf litter ant fauna in Ghana, West Africa (Hymenoptera: Formicidae). J. Hym. Res. 3: 5-16.

Sabu, T. K., Shiju, R. T., Vinod, K. V., and Nithya, S. 2011. A comparison of the pitfall trap, Winkler extractor and Berlese funnel. J. Insect Sci. 11: 28.

Steiner, H. 1962. Methoden zur Untersuchung der Populations Dynamik in Obstanlagen. Entomophaga 7: 207-214.

Vierbergen, G. (Bert), Kucharczyk, H., and Kirk, W. D. J. 2010. A key to the second instar larvae of the Thripidae of the Western Palearctic. Tijd. Entomol. 153: 99-160.

Wilson, T. H. 1975. A monograph of the subfamily Panchaetothripinae (Thysanoptera: Thripidae). Mem. Am. Entomol. Inst. 23:1-354.

Kara Tyler-Julian (1), Ozan Demirozer (1), Richard Figlar (2), Thomas Skarlinsky (3), Joe Funderburk1 and Gary Knox (1)

(1) University of Florida, 155 Research Road, Quincy, Florida 32351 USA E-mail: karatylerjulian@ufl.edu; odemirozer@ufl.edu; jef@ufl.edu; gwknox@ufl.edu

(2) Magnolian Grove Arboretum, 651 Newton Road, Pickens, SC 29671 USA E-mail: magnolia@magnoliaceae.org

(3) USDA-APHIS-PPQ, PO Box 660520, Miami, Florida 33266 USA E-mail: Thomas.L.Skarlinsky@aphis.usda.gov
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