Natural history of the Crambe feeder, Lixus circumcinctus (Coleoptera: Curculionidae).
Abstract: Field investigations showed that Lixus circumcinctus Boheman 1835 (Coleoptera: Curculionidae) is a major herbivore species living on Crambe orientalis in northeastern Anatolia and on Crambe tataria in central Anatolia. Adults hibernate in the root crown of host plants. They associate with their hosts in the spring, feed on leaves, young stems and buds, and lay solitary eggs in the stem and lower leaves of petiole. Larvae open galleries oriented downward toward the root crown. Mature larva make a pupal case using plant fibers at the end of gallery, and pass the pupal stage in the late summer. Second generation adults appear in early autumn, overwinter in the same niche, and complete one generation a year. Crambe orientalis harbors 7 species of weevils in eastern Turkey, and Crambe tataria harbors 8 weevil species in central Anatolia.

Key Words: Crambe, Lixus circumcinctus, biology, host plants, weevil complex

Investigaciones en el campo demostraron que Lixus circumcinctus Boheman 1835 (Coleoptera: Curculionidae) es una especie herbivora de gran importancia que vive en Crambe orientalis en el nororiente de Anatolia, y en Crambe tataria en el centro de Anatolia. Los adultos hibernan en la corona de la raiz de sus plantas hospederas. Estos se asocian con su hospedero en la primavera, cuando se alimentan de hojas, tallos jovenes y brotes, y depositan huevos solitarios en tallos y en peciolos de las hojas bajeras. Las larvas construyen galerias orientadas hacia abajo, en direccion a la corona de la raiz. Al final de la galeria las larvas maduras construyen una camara pupal utilizando fibras de plantas y permanecen en estado pupal durante el verano. Una segunda generacion de adultos aparece al principio del otono, permanece en el mismo nicho durante el invierno, y completa una generacion por ano. Crambe orientalis es hospedera de 7 especies de picudos en el oriente de Turquia, y Crambe tataria, de 8 especies de picudos en el centro de Anatolia.
Article Type: Report
Subject: Animal development (Research)
Authors: Gultekin, Levent
Korotyaev, Boris A.
Pub Date: 12/01/2011
Publication: Name: Florida Entomologist Publisher: Florida Entomological Society Audience: Academic Format: Magazine/Journal Subject: Biological sciences Copyright: COPYRIGHT 2011 Florida Entomological Society ISSN: 0015-4040
Issue: Date: Dec, 2011 Source Volume: 94 Source Issue: 4
Topic: Event Code: 310 Science & research
Geographic: Geographic Scope: Russia; Turkey Geographic Code: 4EXRU Russia; 7TURK Turkey
Accession Number: 298614319
Full Text: The genus Crambe L. (Brassicaceae) consists of about 40 species in the world (Francisco-Ortega et al. 2002); 16 species of Crambe are distributed from East and North Europe to Central Asia (Prina 2009), and 3 species in Turkey (Davis 1982). Crambe species, especially C. abyssinica Hoschst. Ex. R. E. Fries, are prospective industrial and food crops expected to serve as a new source of vegetable oil for food and industrial uses (Gastaldi et al. 1998), as well as an important source of non-woody fibers (Tutus et al. 2010) and as a source of pulp fiber in place of increasingly scarce pulp wood (Jimenez et al. 2005). Crambe spp. May also be exploited as medicinal plants (Sener et al. 1998), wild food plants for humans (Aksakal & Kaya 2008; Dreon & Paoletti 2009), and landscape plants (Karahan & Yilmaz 2001), but they can also be weeds in agricultural fields (Kantar et al. 1999; Qam & Atay 2004; Tursun et al. 2006).

Curculionoidae is a highly diverse superfamily with about 62,000 described species (Oberprieler et al. 2007). Curculionoidae is the largest group of highly specialized phytophagous insects (Korotyaev 2000). Premier Brassicaceae feeder groups include members of Ceutorhynchinae, Baridinae, Lixinae; and mainly the genera Ceutorhynchus Germar 1824, Melanobaris Alonso-Zarazaga & Lyal 1999, Aulacobaris Desbrochers 1892, Lixus Fabricius 1801 (Scherf 1964; Ter-Minassian 1967; Korotyaev & Gultekin 1999; Colonnelli 2004; Gultekin 2007).

The genus Lixus, a member of Lixini tribe, has a nearly worldwide distribution with over 150 species in the Palearctic Region (Csiki 1934; Ter-Minassian 1967). The immature stages of Lixus species are endophagous; they are usually stem, root crown and petiole inhabitants (Scherf 1964; Nikulina 1989; Gultekin 2007; Volovnik 2007), and exceptionally L. obesus Petri 1904 is a seed feeder (Gultekin 2005). The host range includes several families of higher plants such as Chenopodiaceae, Apiaceae and Brassicaceae. The majority of known Lixus species develop on the Brassicaceae; and most of these belong to the subgenus Compsolixus Reitter 1916. Previously Lixus circumcinctus Boheman 1835 was a member of subgenus Lixoglyptus Reitter 1916 (Csiki 1934; Ter-Minassian 1967), however, Lixoglyptus was elevated to genus level and transferred to the tribe Cleonini, but Lixus circumcinctus was left in Lixus 'incertae sedis' by Alonso-Zarazaga (2008).

As a Crambe feeder, L. circumcinctus is distributed in the Azerbaijan, Armenia, Iran and Turkey; where the host plants are C. armena N. Busch in Armenia (Ter-Minassian 1967), and C. orientalis and C. tataria in Turkey (Korotyaev & Gultekin 2003; Gultekin 2007). Another Crambe inhabiting Lixus species is L. canescens Steven 1829, which is associated with Crambe pontica Stev., C. tataria Sebeok, C. steveniana Rupr., C. mitridatis Juz., and C. maritima L. (Ter-Minassian 1967; Volovnik 2007).

In this paper, the ecology of L. circumcinctus and the weevil complex on Crambe is briefly presented.

MATERIAL AND METHODS

Field surveys were conducted in eastern Turkey. Biological observations on Lixus circumcinctus were conducted at Ataturk University Campus [N 39[degrees] 54'.266", E 41[degrees] 12'.962"] (Erzurum Province) at an elevation of 1850 m absl mainly on Crambe orientalis (Figs. 1 and 2). In addition, some observations were conducted during field researches in the Cappadocia territory, Central Anatolia on Crambe tataria (Figs. 3 and 4). To determine the duration of different biological stages, eggs and pupae were collected together with plant parts and held in cages in the laboratory. Photographs of eggs and first instar larvae were taken with a digital camera mounted on an Olympus SZX-7 stereomicroscope.

RESULTS

Adults of Lixus circumcinctus started to leave their overwintering places in the third wk of May. The adults emerged and fed on host plant leaves from the outside to inside, the stems (Fig. 7), lateral branches (Fig. 5) and young flower buds. They damaged the main vein of the leaves to facilitate oviposition and feeding. The adult stage persisted to mid Jun. Most plants each had 2 adults, but sometimes as many as 6. In observing mating pairs, the male proceeded to copulate with the female, while the latter was chewing out an oviposition hole in the stem (Fig. 6).

Beginning in the last wk of May, the L. circumcinctus female laid each egg into a 1-2 mm deep cavity in the stem or at the main vein of the leaf, hole made with her chewing mouthparts at the distal end of her elongated rostrum. The opening of oviposition cavity and the space around the egg was sealed with a sealant containing macerated plant fibers, which the female had prepared from saliva and the tissue that was chewed in the course of excavating the oviposition cavity (Figs. 8 and 9). These sealed oviposition sites turn black in the course of time (Fig. 8). Some plants hosted more than 20 eggs depending on the size of the plant and the number of adults. Egg hatch and emergence of first stage larvae continued for about 2 wk. Eggs could be found in the field until the end of Jun.

First stage larvae (Fig. 10) started to emerge from second wk of Jun. These larvae opened galleries within the stems (Figs. 11 and 12), and tunneled downward to the base of the stem (Fig. 13). The frass, produced by the larvae by consuming the tissue ingested while extending the gallery, was moist and putrified over time. Larvae became mature by the time they had tunneled to the base of the stem, and usually 1 to 5 mature larvae occupied the base of the stem. At this site each mature larva prepared a pupal case by using thin plant fibers (Fig. 14). Mature larvae were present throughout Aug. Pupation in the pupal cases occurred during the last 2 wk of Aug (Fig. 14), and the duration of pupal period was about 2 wk.

Transformation of pupae into adults occurs in the pupal case (Fig. 15) beginning in the latter part of Aug and ending early in Sep. The adults overwintered in the same place. Thus, L. circumcinctus has only one generation a year.

Besides L. circumcinctus, weevils associated with Crambe orientalis L. in eastern Turkey are Aulacobaris coerulescens (Scopoli 1763), Melanobaris crambephaga (Korotyaev & Gultekin 1999), Ceutorhynchus pallidactylus (Marsham 1802), Ceutorhynchus erivanus Schultze 1898, Ceutorhynchus fabrilis Faust 1887, Ceutorhynchus sophiae Gyllenhal 1837, whereas those associated with Crambe tataria Sebeok in central Anatolia are Bruchela suturalis (Fabricius 1792), Aulacobaris janthina (Boheman 1836), Melanobaris sp. nr. semistriata (Boheman 1836), Ceutorhynchus chalybaeus Germar 1824, Ceutorhynchus picitarsis Gyllenhal 1837, Ceutorhynchus sulcicollis (Paykull 1800).

DISCUSSION

Lixus circumcinctus is one of the larger Lixus in the Palaearctic with conspicuous white stripes, complete on 10th interval, and limited to apical half of 9th interval in the form of very narrow line on each ventral margin, whereas white stripes are vague on the 11th intervals. These features are shared with species of the subgenus Compsolixus, namely intervals 9th-11th with white stripes, and perhaps its inclusion into this subgenus would be appropriate. However, although the majority of Compsolixus are associated with Brassicaceae, the type species of this subgenus Lixus juncii Boheman 1835 is a pest of Beta vulgaris L. which is a Chenopodiaceae. Determining the right position of Lixus circumcinctus, and placing it in a subgenus already described, or erecting a new subgenus should be done after a supraspecific level phylogenetic analysis of the Lixini, which is beyond the scope of this paper.

[FIGURES 1-7 OMITTED]

[FIGURES 8-15 OMITTED]

This is the first biological study of L. circumcinctus which is one of the large herbivores on Crambe plants. As mentioned above, the importance of this plant group is increasing its suitability for a broad spectrum of uses. In addition, Crambe plants play an important role with respect to biodiversity by serving food, ecological niches and harborage for many insect species such as pollinators, natural enemies and herbivores.

ACKNOWLEDGMENTS

We express heartfelt thanks to Professor Vladimir I. Dorofeyev and Professor Huseyin Zengin for identification of plants, and to an anonymous reviewer for highly valuable linguistic contributions. The study was supported by the Collaborative Linkage Grant No. 978845 and NR-CLG-981318 of the NATO Life Science and Technology Programme.

REFERENCES CITED

ALONSO-ZARAZAGA, M. A. 2008. Notes on enigmatic weevil taxa described from Spain and Morocco (Coleoptera, Curculionidae). Zootaxa 1806: 66-68.

AKSAKAL, O., AND KAYA, Y. 2008. Erzurum ve cevresinde halk tarafindan gida amacli olarak kullanilan bitkiler. Turkiye 10, Gida Kongresi; 21-23 Mayis 2008, Erzurum, pp. 1009-1012.

COLONNELLI, E. 2004. Catalogue of Ceutorhynchinae of the world, with a key to genera (Insecta: Coleoptera: Curculionidae). Argania editio, Barcelona, 124 pp.

CAM, H., AND ATAY, T. 2004. The leaf-beetles (Coleoptera, Chrysomelidae) that feed on some weeds in Tokat Province. GOU. Ziraat Fakultesi Dergisi 21(2): 7-14 (in Turkish).

CSIKI, E. 1934. Coleopterorum Catalogus auspiciis et auxilio W. Junk editus a S. Schenkling. Pars 134. Curculionidae: subfam. Cleoninae. Junk, Berlin, 152 pp.

DAVIS, P. H. 1982. Flora of Turkey and East Aegean Islands. Vol. 1, pp: 272-273. Edinburgh University Press.

DREON, A. L., AND PAOLETTI, M. G. 2009. The wild food (plants and insects) in Western Friuli local knowledge (Friuli-Venezia Giulia, North Eastern Italy). Contributions to Natural History 12: 461-488.

FRANCISCO-ORTEGA, J., FUERTES-AGUILAR, J., KIM, S G., SANTOS-GUERRA, A., CRAWFORD, D. J., AND JANSEN, R. K. 2002. Phylogeny of the Macaronesian endemic Crambe section Dendrocrambe (Brassicaceae) based on internal transcribed spacer sequences of nuclear ribosomal DNA. Am. J. Botany 89(12): 1984-1990.

GASTALDI, G., CAPRETTI, G., FOCHER, B., AND COSENTINO, C. 1998. Characterization and proprieties of cellulose isolated from the Crambe abyssinica Hull. Industrial Crops Prod. 8: 205-218.

GULTEKIN, L. 2005. New ecological niche for weevils of the genus Lixus Fabricius and biology of Lixus obesus Petri (Coleoptera: Curculionidae, Lixinae). Weevil News: No. 24: 3 pp. http://www.curci.de/Inhalt.html.

GULTEKIN, L. 2007. Oviposition niches and behavior of the genus Lixus Fabricius (Coleoptera: Curculionidae, Lixinae). Entomol. Fennica 18: 74-81.

JIMENEZ, L., RAMOS, E., RODRIGUEZ, A., DE LA TORRE, M. J., AND FERRER J. L. 2005. Optimization of Pulping conditions of abaca. An alternative raw material for producing cellulose pulp. Bioresource Tech. 96: 977-983.

KANTAR, F., ELKOCA, E., AND ZENGIN, H. 1999. Chemical and Agronomical Weed Control in Chickpea (Cicer arietinum L. cv. Aziziye-94). Turkish J. Agric. Forest 23: 631-635.

KARAHAN, F., AND YILMAZ, H. 2001. Determination of some alpine plants suitable for landscape planning in Erzurum and surroundings. Turkish J. Agric. Forest. 25: 225-233.

KOROTYAEV, B. A. 2000. On unusual high diversity of Rhynchophorus beetles (Coleoptera, Curculionidae) in steppe communities of the North Caucasus. Entomol. Rev. 80(8): 1022-1026.

KOROTYAEV, B. A., AND GULTEKIN, L. 1999. A new species of weevil genus Baris from NE Turkey (Coleoptera: Curculionidae). Zoosystematica Rossica 8(1):141-142.

KOROTYAEV, B. A., AND GUTEKIN, L. 2003. Biology of two weevils, Lixus ochraceus Boheman and Melanobaris gloriae sp. nov. (Insecta: Coleoptera, Curculionidae), associated with Tchihatchewia isatidea Boissier, a cruciferous plant endemic of Turkey. Entomol. Abhandl. 61(1): 93-99.

NIKULINA, O. N. 1989. Biology of weevils of the genus Lixus (Coleoptera, Curculionidae) developing in semishrub and herbaceous plants in Tajikistan. Entomol. Obozr. 3: 511-521.

OBERPRIELER, R. G., MARVALDI, A. E., AND ANDERSON R. S. 2007. Weevils, weevils, weevils everywhere. Zootaxa 1668: 491-520.

PRINA, A. 2009. Taxonomic review of the genus Crambe sect. Crambe (Brassicaceae, Brassiceae). An. Jardin Botanico de Madrid 66(1): 7-24.

SCHERF, H. 1964. Die Entwicklungsstadien der mitteleuropaischen Curculioniden (Morphologie, Bionomie, Okologie). Abhandl. Senckenbergischen Naturforsch. Gesell. 506: 1-335.

STEVEN, C. von. 1829: Notice sur quelques Insectes de la collection de C. Steven. Bull. Soc. Imper. Natural. Moscou 1(9): 284-288.

SENER, B., BINGOL, F., ERDOGAN, I., BOWERS, W. S., AND EVANS P. H. 1998. Biological activities of some Turkish medicinal plants. Pure and Applied Chemistry 70(2): 403-406.

TER-MINASSIAN, M. E. 1967. Zhuki-dolgonosiki podsemejstva Cleoninae fauny SSSR. Tsvetozhily i stebleedy (triba Lixini). Nauka, Leningrad, 140 [+ 1 unnumbered] p. (Translated 1978. Weevils of the subfamily Cleoninae in the fauna of the USSR. Tribe Lixini. ARS-USDA and National Science Foundation, Washington. Amerind Publishing Co., New Delhi, vi + 166 p.)

TURSUN, N., KANTARCI, Z., AND SEYITHANOGLU, M. 2006. Determination of weed seeds contaminating wheat grain in Kahramanmaras, Province. Kansas State Univ. J. Sci. Engineer. 9(2): 110-115.

TUTUS, A., COMLEKCIOGLU, N., KARAMAN, S., AND ALMA, M. H. 2010. Chemical composition and fiber properties of Crambe orientalis and Crambe tataria. International Journal of Agriculture & Biology 12: 286-290.

VOLOVNIK, S. V. 2007. On distribution and ecology of some species of Cleonines (Coleoptera, Curculionidae): IV. Genus Lixus F., subgenus Eulixus Reitt. Entomol. Rev. 87(7): 840-847 [Original Russian Text [c] S.V. Volovnik, 2007, published in Entomol. Obozr. Vol. 86(3) pp. 521-531].

LEVENT GULTEKIN (1) AND BORIS A. KOROTYAEV (2)

(1) Ataturk University, Faculty of Agriculture, Department of Plant Protection, 25240 Erzurum, Turkey E-mail: lgultekin@gmail.com

(2) Zoological Institute, Russian Academy of Sciences, St. Petersburg 199034, Russia E-mail: baris@zin.ru

Supplementary material online at http://www.fcla.edu/FlaEnt/fe944.htm#infoLink4
Gale Copyright: Copyright 2011 Gale, Cengage Learning. All rights reserved.