J Zoo Wildl Med.: Application of galactomannan analysis and protein electrophoresis in the diagnosis of aspergillosis in avian species.
Article Type: Brief article
Subject: Antigens (Testing)
Antigens (Health aspects)
Aspergillosis (Diagnosis)
Blood protein electrophoresis (Usage)
Birds (Health aspects)
Animals (Diseases)
Animals (Diagnosis)
Authors: Cray, C.
Watson, T.
Rodriguez, M.
Arheart, K.L.
Pub Date: 06/01/2009
Publication: Name: Journal of Avian Medicine and Surgery Publisher: Association of Avian Veterinarians Audience: Academic Format: Magazine/Journal Subject: Health Copyright: COPYRIGHT 2009 Association of Avian Veterinarians ISSN: 1082-6742
Issue: Date: June, 2009 Source Volume: 23 Source Issue: 2
Topic: Event Code: 330 Product information
Product: SIC Code: 2836 Biological products exc. diagnostic
Geographic: Geographic Scope: United States Geographic Code: 1USA United States
Accession Number: 252006961
Full Text: Previous studies support the possible application of galactomannan, a major antigen of Aspergillus species, to aspergillosis diagnosis in avian and other animal species. An assay is commercially available for use with human serum and bronchoalveolar lavage fluid samples. In the current study, galactomannan results from plasma samples were compared between birds with histologically confirmed aspergillosis and those that were clinically normal presumptively non-Aspergillus infected birds per submitting practitioners' responses to a questionnaire. It was observed that infected birds demonstrated a 2.6-fold increase in galactomannan over birds without evidence of aspergillosis. With the use of a galactomannan index of 0.5 as a cutoff, the sensitivity and specificity of the test were found to be 67% and 73%, respectively. In addition, plasma samples were analyzed for abnormalities in protein electrophoretic patterns. Infected birds had a higher incidence of increased beta and/or gamma globulin concentrations. Test sensitivity and specificity were 73% and 70%, respectively. If the 2 tests were used as a panel, then the sensitivity was 89% and specificity was 48%. These data indicate that both galactomannan and protein electrophoresis may be valuable tools in the diagnosis of avian aspergillosis.

2009;40:64-70.
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