Immunoglobulin E.
Subject: Immunoglobulin E (Physiological aspects)
Authors: Winter, William E.
Hardt, Nancy S.
Fuhrman, Susan
Pub Date: 09/01/2000
Publication: Name: Archives of Pathology & Laboratory Medicine Publisher: College of American Pathologists Audience: Academic; Professional Format: Magazine/Journal Subject: Health Copyright: COPYRIGHT 2000 College of American Pathologists ISSN: 1543-2165
Issue: Date: Sept, 2000 Source Volume: 124 Source Issue: 9
Geographic: Geographic Scope: United States Geographic Code: 1USA United States
Accession Number: 65639706
Full Text: Importance in Parasitic Infections and Hypersensitivity Responses

Immunoglobulin E (IgE) is one of the body's 5 classes (isotypes) of immunoglobulins (antibodies). Like other immunoglobulins, IgE is produced by B cells and plasma cells.[1,2] In contrast to other immunoglobulins, the concentration of IgE in the circulation is very low. Immunoglobulin E in cord blood usually measures less than 1 U/mL (1 U = 2.4 ng). Generally, adult IgE levels are achieved by 5 to 7 years of age. Between the ages of 10 and 14 years, IgE levels may be higher than those in adults. After age 70 years, IgE levels may decline slightly and be lower than the levels observed in adults younger than 40 years.

Circulating IgE concentrations are very low because mast cells have a very high affinity for IgE ([10.sup.10] mol/[L.sup-1]) via their [Epsilon]-heavy-chain Fc receptors (Fc[Epsilon]R). The synthetic rate for IgE is also very low. Immunoglobulin E attaches to mast cells and to basophils and activated eosinophils. Immunoglobulin E on mast cells has a half-life ([T.sub.1/2]) of more than 10 days.

In contrast to other immunoglobulins that bind to immunoglobulin Fc receptors only when antigen has been bound by an antibody, IgE will bind to Fc[Epsilon]R in the absence of antibody. Immunoglobulin E binding to mast cells "sensitizes" the mast cells to degranulate when multivalent antigens cross-link Fc[Epsilon]R-bound IgE (Figure). Intrinsically innocuous antigens that produce hypersensitivity responses are termed allergens.

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MAST CELL FUNCTION AND THE PROTECTIVE EFFECTS OF IgE

Mast cells play several roles in host defense. As the origin of many proinflammatory substances, mast cell degranulation-mediated inflammation allows circulating cells and plasma proteins increased access to interstitial spaces to combat infection. When antigens (or in the case of allergies, allergens) cross-link IgE on mast cell surfaces (eg, cause aggregation of IgE and Fc[Epsilon]R because the antigen is polyvalent, ie, expresses multiple identical epitopes), mast cell degranulation is triggered. Degranulation releases histamine and other biologic substances that can induce coughing, sneezing, vomiting, or diarrhea. These actions serve to expel pathogens from the body. Such pathogens include metazoan parasites. Parasites are classified as protozoans (single-cell parasites) or metazoans (multicell parasites). Parasitic infection can involve the gastrointestinal tract, lungs, blood stream, or solid organs. Metazoans are called helminths (worms). Elevated IgE levels occur in several helminthic infections. The helminths are divided into the flatworms (Platyhelminthes) and the roundworms (nematodes [Nemathelminthes]). The medically important flatworms are further divided into the flukes (Trematoda) and tapeworms (Cestoda). Tapeworms live in the gut but also can infect solid organs. Flukes live in the blood vessels, lungs, or liver. Examples of the consequences of parasitism include anemia, development of space-occupying lesions or granulomas in solid organs, allergic reactions, obstruction of blood vessels or lymphatics, induction of cancer, blindness, and diarrhea.

Immunoglobulin E also can induce an antibody-dependent, cell-mediated cytotoxic response against helminthic parasites. Immunoglobulin E that binds to such parasites focuses eosinophil targeting against the parasites. Once IgE is bound to the helminthic parasite and eosinophils bind to IgE, the eosinophil can degranulate against the parasite. Helminthic parasites are too large to be phagocytized. The toxic products of the eosinophil granules can either kill, damage, or dislodge the parasite as a protective host mechanism. Therefore, it is common to observe both elevated IgE levels and eosinophilia in many helminthic parasitic infections (see "Differential Diagnosis of Elevated IgE Concentrations"). In summary, (1) IgE binds to the target parasite antigen, (2) the eosinophil FcR[Epsilon] receptor binds to Fc[Epsilon] of IgE, (3) the eosinophil degranulates toward the parasite, and (4) toxic products of the released eosinophil granules damage, destroy, or dislodge the parasite.

PATHOLOGIC EFFECTS OF IgE

In industrialized populations in which the frequency of helminthic parasitic infection is low, the adverse actions of IgE are manifested as a high frequency of type I hypersensitivity.[3,4] While allergy (hypersensitivity) was unusual at the turn of the century, allergies now affect up to 20% of adults. Approximately 5% of children have asthma, 15% of children have allergic rhinitis, 5% have eczema, and possibly up to 40% of all children are affected by minor allergic conditions. Experts hypothesize that the decreasing frequency of parasitism, which has occurred as a consequence of improved sanitation and hygiene, has left the immune system "unoccupied," thereby fostering immune responses to benign antigens (eg, allergens) that produce allergic responses. Examples of type I hypersensitivity reactions are listed in Table 1.

DIFFERENTIAL DIAGNOSIS OF ELEVATED IgE CONCENTRATIONS

Circulating IgE levels are predominantly elevated in helminthic parasitic and allergic conditions. Table 2 provides a list of conditions in which elevated IgE levels may be found. As noted, in industrialized countries, allergy is the most common cause of elevated IgE concentrations, whereas in lesser developed, predominantly agrarian countries, parasitic infection is the most common cause of elevated IgE levels. Elevated IgE levels can be detected in some individuals before the expression of clinical allergy. However, the usefulness of such testing is unclear because no prophylactic immunotherapy exists to prevent the development of allergy.

Table 2. Differential Diagnosis of Elevated Immunoglobulin E (IgE)

While elevated IgE levels can be consistent with various allergic conditions, to determine the precise allergen to which an individual is sensitive, either radioallergosorbent testing (RAST) or skin testing should be carried out. Radioallergosorbent testing detects the presence of IgE sensitivity to specific allergens. Skin testing is performed by scratching or pricking the skin and applying a solution that contains a specific allergen over the abrasion. If the individual has mast cells under their skin sensitized with IgE specific for the allergen, an immediate hypersensitivity wheal and flare (eg, hive) response will develop within 15 to 20 minutes. Using skin testing, allergists can test for IgE antibody to 50 or more allergens simultaneously by applying multiple solutions to individual sites over the patient's back.

The advantage of RAST testing is that it can be performed simply by taking a blood specimen. However, in performing RAST testing for a large battery of allergens, the overall cost becomes substantial. Advantages of skin testing are that (1) skin testing more closely relates to clinical disease than RAST testing and (2) since an allergist performs the testing in a clinic, the results are available immediately.

IgE DEFICIENCY

It is difficult to define IgE deficiency because IgE levels are normally very low. Immunoglobulin E deficiency can be defined as IgE levels less than 2 U/mL in children and less than 4 U/mL in adults. Low IgE levels have been reported in various forms of severe combined immunodeficiency, hyper-IgM syndrome, ataxia telangiectasia, X-linked recessive Bruton agammaglobulinemia, common variable immunodeficiency, transient hypogammaglobulinemia of infancy, and isolated IgE deficiency whose clinical significance is unclear. Some experts in immunodeficiency diseases recommend that IgE be measured if an antibody deficiency state is being considered.

ANALYTICAL ISSUES IN THE MEASUREMENT OF IgE

Solid-phase displacement radioimmunoassays, double-antibody radioimmunoassays, solid-phase sandwich radio-immunoassays, and nephelometry are all used to measure IgE. In most assays, the lower limit of detection is 0.5 U/mL (1.2 ng/mL). According to the Clinical Laboratory Improvement Amendments of 1988 (CLIA '88), acceptable control ranges are within [+ or -] 3 SD of the mean control value. The overall precision goal should be [+ or -] 0.75 SD of the mean. The maximum total error (fixed limit goal) according to CLIA '88 is [+ or -] 3 SD of the mean.

CLINICAL USE OF IgE MEASUREMENTS

The suggested clinical application of IgE measurements is outlined in Table 3. An elevated IgE concentration is not diagnostic of any single condition. However, there are at least 3 conditions (IgE monoclonal gammopathy, hyper-IgE syndrome, and allergic bronchopulmonary aspergillosis) in which elevated IgE levels are universally observed. A normal IgE level excludes an IgE monoclonal gammopathy, hyper-IgE syndrome, and allergic bronchopulmonary aspergillosis. Depressed or elevated IgE levels are sought frequently in suspected immunodeficiency disorders.

Table 3. Clinial Use of Immunoglobulin E (IgE) Measurements

An elevated IgE concentration frequently is found in various allergic and helminthic parasitic diseases, as outlined in Table 2. An elevated IgE level would support the diagnosis of an allergic or helminthic parasitic disorder, but a normal IgE concentration would not exclude the specific diagnosis under consideration. If the diagnosis of allergy or helminthic parasitism is unclear, an otherwise unexplained elevated IgE value may be helpful in suggesting allergy or parasitism. However, if the diagnosis of allergic or helminthic parasitic disease is likely, measurement of IgE is not likely to provide substantial additional information.

In infants, IgE levels greater than 20 U/mL support the diagnosis of allergic rhinitis; however, a normal IgE value does not rule out allergic conditions. Immunoglobulin E levels in adults are less helpful in establishing an allergic etiology for symptoms. In patients with suspected allergic conditions, IgE levels greater than +1 SD above the mean "suggest" allergic disease, while values greater than +2 SD above the mean "strongly suggest" allergic disease. Measuring the total IgE concentration is not usually helpful once the diagnosis of allergic disease has been established clinically, whereas an elevated IgE value has only limited power in predicting allergic tendencies. Therefore, measuring IgE in the latter 2 situations is not recommended.

Finally, elevated IgE levels are observed in many inflammatory and infectious diseases. Immunoglobulin E measurements are usually not helpful in such conditions, and IgE measurements are rarely indicated.

SUMMARY

Compared with the major circulating immunoglobulins (IgG, IgA, and IgM), IgE normally occurs in very low concentrations in the circulation. The normal concentration of IgE is only 0.05% of the IgG concentration. Because immunoassay procedures are necessary to measure the low circulating concentrations of IgE, IgE proficiency testing is included in the general ligand surveys of the College of American Pathologists (K, KN [K with SI unit reporting], and KK [K with a duplicate set of vials]). This review of IgE focused on the biology of IgE, the differential diagnosis of elevated IgE concentrations, the measurement of IgE, and the clinical indications for the measurement of IgE.

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References

[1.] Leung DYM. Immunologic basis of chronic allergic disease: clinical messages from the laboratory bench. Pediatr Res. 1997;42:559-568.

[2.] Leung DYM. Molecular basis of allergic disease. Mol Genet Metab. 1998; 63:157-167.

[3.] Homburger HA. Allergic diseases. In: Henry JB, ed. Clinical Diagnosis and Management by laboratory Methods. 19th ed. Philadelphia, Pa: WB Saunders; 1996:1051-1063.

[4.] Allergy and hypersensitivity. In: Janeway CA, Travers P, Walport M, Capra JD, eds. Immunobiology, the Immune System in Health and Disease. 4th ed. New York, NY: Current Biology Publications; 1999:461-488.

Accepted for publication March 31, 2000.

From the Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, College of Medicine, Gainesville, Fla (Drs Winter and Hardt), and the Department of Pathology, Grant Riversides Methodist Hospital, Columbus, Ohio (Dr Fuhrman).

Reprints: William E. Winter, MD, Department of Pathology, Laboratory Medicine, and Immunology, University of Florida, College of Medicine, Box 100275, Gainesville, FL 32610-0275.
Table 1. Clinical Examples of Type I Hypersensitivity Disorders

Disorder           Clinical Manifestations   Allergens

Systemic           Edema, vasodilation,      Drugs, serum, venoms
anaphylaxis        tracheal mucosal
                   swelling with
                   occlusion, circulatory
                   collapse, death

Wheal and flare    Local vasodilation and    Insect bites,
responses          edema                     allergy testing

Allergic           Edema and irritation of   Pollens (ragweed,
rhinitis           nasal mucosa              timothy, birch),
(hay fever)                                  mite feces

Bronchial asthma   Bronchial constriction,   Pollens, dust mite
                   mucosal edema from        feces
                   inflammation, excessive
                   mucus production

Food allergy       Vomiting, diarrhea;       Shellfish, milk,
                   pruritus, urticaria       fish, wheat,
                   (hives: allergen          legumes, corn,
                   travels to skin)          citrus fruit,
                                             eggs, tomatoes

                   Route of
Disorder           Exposure

Systemic           Intravenous
anaphylaxis

Wheal and flare    Subcutaneous
responses

Allergic           Inhalation
rhinitis
(hay fever)

Bronchial asthma   Inhalation

Food allergy       Oral


Category                     Examples

Allergic disease             Atopic dermatitis (eczema)
                             Allergic rhinitis
                             Allergic asthma
                             Extrinsic allergic alveolitis
                             Drug allergies
                             Allergic urticaria

Parasitic disease
  Cestodes                   Echinococcus granulosus
                             Echinococcus multiocularis
  Trematodes                 Schistosoma mansoni
                             Schistosoma japonicum
                             Schistosoma haematobium
  Nematodes                  Ascaris lumbricoides
                             Ancylostoma caninum
                             Ancylostoma braziliense
                             Capillaria philippinensis
                             Toxocara canis
                             Toxocara cati

Immunologic disorders
  Monoclonal gammopathy      IgE monoclonal gammopathy
  Immune deficiency states   Hyper-IgE syndrome
                             Wiskott-Aldrich syndrome
                             DiGeorge syndrome
                             Nezelof syndrome
                             Graft versus host disease
                             Acquired immunodeficiency
                               syndrome
                             Cystic fibrosis
  Inflammatory diseases      Kawasaki disease
                             Periarteritis nodosa

Infectious diseases          Leprosy
                             Bronchopulmonary aspergillosis
                             Aspergilloma


Measurement of IgE always indicated in patients with suspected
  IgE monoclonal gammopathy
  Hyper-IgE syndrome
  Allergic bronchopulmonary aspergillosis
  Immunodeficiency syndromes

Measurement of IgE sometimes indicated in patients with
suspected
  Allergic disease
  Helminthic parasitism

Measurement of IgE usually not indicated in patients with
suspected
  Inflammatory diseases
  Nonparasitic infectious diseases
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