Escherichia coli producing CMY-2 [beta]-Lactamase in retail chicken, Pittsburgh, Pennsylvania, USA.
Article Type: Letter to the editor
Subject: Drug resistance in microorganisms (Research)
Escherichia coli (Research)
Beta lactamases (Research)
Authors: Park, Yoon Soo
Adams-Haduch, Jennifer M.
Rivera, Jesabel I.
Curry, Scott R.
Harrison, Lee H.
Doi, Yohei
Pub Date: 03/01/2012
Publication: Name: Emerging Infectious Diseases Publisher: U.S. National Center for Infectious Diseases Audience: Academic; Professional Format: Magazine/Journal Subject: Health Copyright: COPYRIGHT 2012 U.S. National Center for Infectious Diseases ISSN: 1080-6040
Issue: Date: March, 2012 Source Volume: 18 Source Issue: 3
Topic: Event Code: 310 Science & research
Geographic: Geographic Scope: United States Geographic Code: 1USA United States
Accession Number: 283455643
Full Text: To the Editor: Rates of resistance to various antimicrobial drugs are rapidly increasing in Escherichia coli, not only in health care settings but also in the community. The food supply is suspected as a potential source of antimicrobial-resistant E. coli strains, which include cephalosporin-resistant E. coli found in retail meat products and other types of food (1).

We reported a high prevalence of cephalosporin-resistant E. coli, most of which produced CMY-2 [beta]-lactamase, among retail poultry products in Pittsburgh, Pennsylvania, USA during 2006-2007 (2). CMY-2 is the most commonly acquired ampicillin C (AmpC)-type [beta]-lactamase found in E. coli that cause human infections (3). The aim of this study was to investigate whether cephalosporin-resistant E. coli are present in retail raw meat and ready-to-eat meat products in our area 5 years after our previous study and define subtypes of concern.

A convenience sampling of 104 raw ground meat products from 3 local grocery stores in Pittsburgh was performed during February-April 2011. Items purchased were samples of all available deli counter ground meat, all fresh sausages prepared in stores at the deli counter, and selected uncooked commercially packaged fresh and frozen sausages. Types of meat items were chicken (n = 22), turkey (n = 10), lamb (n = 2), pork (n = 43), and beef (n = 27).

Approximately 10 gof each sample was excised and suspended in 10 mL of nutrient broth. After being incubated overnight at 37[degrees]C, 10 [micro]L of broth was plated on MacConkey agar plates containing 2 mg/L of cefotaxime or ceftazidime, and the plates were incubated overnight at 37[degrees]C. Lactose-fermenting colonies were identified as E. coli by using standard biochemical methods, which included sulfide indole motility, growth on triple sugar iron medium, oxidase activity testing, and the API20E system (bioMerieux, Durham, NC, USA) as needed.

Antimicrobial drug susceptibility was determined by using the disk diffusion method (AB Biodisk, Solna, Sweden) according to the manufacturer's instructions and interpreted according to the criteria of the Clinical and Laboratory Standards Institute (4). Isolates were screened for extended-spectrum [beta]-lactamase (ESBL) production by using the double-disk diffusion method and for acquired ampC-type [beta]-lactamase genes by using multiplex PCR (4,5).

Phylogenetic groups (A, B1, B2, and D) were determined as reported (6). Screening for sequence type (ST) 131 was conducted by using PCR and confirmed by using multilocus sequence typing (7,8). Pulsed-field gel electrophoresis was performed to determine clonal relationships by using XbaI and the protocol available through the PulseNet ( protocols.htm). Banding patterns were analyzed by using BioNumerics software version 6.01 (Applied Maths, Sint-Martens-Latem, Belgium).

Among 104 meat samples, 9 contained cephalosporin-resistant E. coli, resulting in an overall prevalence of 8.7% (95% CI 4.0%-15.8%). Cephalosporin-resistant E. coli was isolated from 7 (31.8%) of 22 chicken, 1 (10.0%) of 10 turkey, and 1 (2.3%) of 42 pork samples. No cephalosporin-resistant E. coli was detected from beef and lamb samples. Incidence of samples with cephalosporin-resistant E. coli was lower than in our previous study (2). This finding may be caused by different types of samples included in the studies or a true decrease in incidence.

Features of cephalosporinresistant E. coli identified are summarized in the Table. None produced ESBL, but all 9 isolates were positive for the CMY-2 [beta]-lactamase gene and positive results were confirmed by sequencing. CMY 2 is the most commonly observed acquired AmpC [beta]-lactamase in E. coli and nontyphoidal Salmonella species in meat products (9).

As for the phylogenetic groups, 6 (66.7%) of 9 cephalosporin-resistant E. coli belonged to group A, which is generally considered to be a commensal phylogenetic group. However, 1 group B2 isolate from a chicken sample was identified as ST131. The CMY-2 gene was located on an IncI1-type plasmid and was transferable to another E. coli strain by conjugation for this isolate. Two group D isolates from chicken that belonged to ST117, which has been reported in ESBL-producing isolates of human and animal origins (10); no clonality was observed for the other 7 isolates by pulsed-field gel electrophoresis,

E. coli ST131 has emerged as a worldwide pathogen and causes mainly community-onset extraintestinal infections. Although the pandemic spread of E. coli ST131 was first identified in isolates producing CTX-M-15 ESBL, it is increasingly recognized that isolates belonging to this clone may also harbor other drug resistance determinants. Among acquired AmpC [beta]-lactamases, CMY-2 has been most frequently reported in ST131 from human clinical isolates (3). Infections caused by CMY-producing E. coli are common but underrecognized because of the lack of standardized detection methods (2).

Given the rapid global spread of the ST131 clone and the possibility of its transmission from food animals to humans, coupled with an abundance of CMY-2-encoding plasmids in poultry environments, E. coli ST131 producing CMY-2 [beta]-lactamase may have potential to spread to humans. Our results also show that E. coli producing CMY-2 continues to be found commonly among retail chicken products in our study area.

This study was supported in part by grants from the Pennsylvania Department of Health (grant 4100047864), the National Institute of Allergy and Infectious Diseases (NIAID; R03AI079296), and career development awards from NIAID to L.H.H. (K24AI52788) and Y.D. (K22AI080584).


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Yoon Soo Park, Jennifer M. Adams-Haduch, Jesabel I. Rivera, Scott R. Curry, Lee H. Harrison, and Yohei Doi

Author affiliations: University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA (Y.S. Park, J.M. Adams-Haduch, J.I. Rivera, S.R. Curry, L.H. Harrison, Y. Doi); University of Pittsburgh Graduate School of Public Health and School of Medicine, Pittsburgh (L.H. Harrison); and Gachon University Gil Hospital, Incheon, South Korea (Y.S. Park)


Address for correspondence: Yohei Doi, Division of Infectious Diseases, University of Pittsburgh School of Medicine, Scaife Hall S829, 3550 Terrace St, Pittsburgh, PA 15261, USA; email:
Table. Characteristics of 9 cephalosporin-resistant Escherichia coli
isolates in retail meat, Pittsburgh, Pennsylvania, USA *

Sample                       Phylogenetic
no.           Origin            group

FD13     Chicken (ground)         D
FD14      Pork (sausage)          A
FD42     Chicken (sausage)        B2
FD44      Chicken (thigh)         A
FD45     Turkey (sausage)         A
FD56     Chicken (sausage)        D
FD63     Chicken (sausage)        A
FD72     Chicken (sausage)        A
FD95      Chicken (liver)         A

no.      CTX   FOX   FEP   CIP   GEN   TET

FD13      R     I     S     S     S     S
FD14      R     R     S     S     S     R
FD42      R     R     S     S     S     S
FD44      R     R     S     S     S     S
FD45      R     R     S     S     R     R
FD56      R     R     S     S     S     S
FD63      R     R     S     S     S     S
FD72      R     R     S     S     S     R
FD95      R     I     S     S     S     S

* All isolates were CMY-2 type. CTX, cefotaxime; FOX, cefoxitin; FEP,
cefepime; CIP, ciprofloxacin; GEN, gentamicin; TET, tetracycline; R,
resistant; I, intermediate; S, susceptible.
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