Effects of fattening period on growth performance, carcass characteristics and lipogenic gene expression in Hanwoo steers.
|Abstract:||This study was conducted to investigate the effects of different fattening periods i.e. 25, 27 and 29 months of age (25 mo, 27 mo and 29 mo), on feed consumption, body weight gain, carcass parameters, and lipogenic gene expression in 45 Korean native steers (Hanwoo). Daily DM intake was higher in steers on 29 mo compared with those on 25 mo or 27 mo. Daily body weight gain was higher in steers on 25 mo compared with those on 27 mo or 29 mo during fattening and overall experimental periods. Therefore, feed conversion ratio was lower in 25 mo compared with 27 mo or 29 mo during the fattening and whole experimental periods. As expected, slaughter and carcass weights were higher in the order of 29 mo>27 mo>25 mo. Carcass yield grade was relatively lower in 29 mo reflecting higher back fat thickness compared with other treatments, while carcass quality grade was not largely influenced by the treatments. By investigation with an ultra-sound scanning technique, the marbling score was significantly and numerically higher in 25 mo compared with 27 mo or 29 mo. The mRNA levels of stearoyl-CoA desaturase (SCD) gene were gradually increased in the late fattening stages (p<0.01) and mRNA of acetyl-CoA carboxylase (ACC), ATP citrate lyase (ACL) and glucose transporter 4 (GLUT4) gene were highly expressed in 29 mo compared with 25 mo and 27 mo (p<0.05). However, gene expressions of adipocyte fatty acid binding protein 4 (FABP4) and lipoprotein lipase (LPL) were not significantly different among the treatments. Thus the present results indicated that different fattening period has no major effect on carcass characteristics, although 25 mo had a lower carcass weight compared with 27 mo or 29 mo. (Key Words : Hanwoo, Steers, Fattening, Growth, Carcass Characteristics, Lipogenic Gene)|
Fat metabolism (Genetic aspects)
Gene expression (Research)
Cattle (Genetic aspects)
Kwon, Eung Gi
Park, Byung Ki
Kim, Hyeong Cheol
Cho, Young Moo
Kim, Tae Il
Chang, Sun Sik
Oh, Young Kyoon
Kim, Nam Kuk
Kim, Jun Ho
Kim, Young Jun
Im, Seok Ki
|Publication:||Name: Asian - Australasian Journal of Animal Sciences Publisher: Asian - Australasian Association of Animal Production Societies Audience: Academic Format: Magazine/Journal Subject: Agricultural industry; Biological sciences Copyright: COPYRIGHT 2009 Asian - Australasian Association of Animal Production Societies ISSN: 1011-2367|
|Issue:||Date: Dec, 2009 Source Volume: 22 Source Issue: 12|
|Topic:||Event Code: 310 Science & research|
|Product:||Product Code: 0212000 Beef Cattle & Calves; 2011112 Whole Carcass Beef NAICS Code: 1121 Cattle Ranching and Farming; 31161 Animal Slaughtering and Processing SIC Code: 0211 Beef cattle feedlots; 0212 Beef cattle, except feedlots; 2011 Meat packing plants|
|Geographic:||Geographic Scope: South Korea Geographic Code: 9SOUT South Korea|
Bovine carcass characteristics and thus beef quality are affected by various factors such as age, sex, genetics, and nutrition. In addition, meat quality is also determined by meat color, fat color, texture, and marbling (intramuscular fat) scores. Marbling plays a particularly important role in determining the juiciness and tenderness of beef, and is one of the main factors used to determine beef quality grade in Korea (Lee et al., 2001; Lee, 2004). Tatum et al. (1982) reported that marbling has been implicated as a contributing factor to beef palatability, and is used as one of the most important factor in evaluating the beef quality.
Hanwoo steers dramatically increase their marbling fat in muscle between 12 and 27 months of age (Lee et al., 2007). Generally, Hanwoo has been fattened until almost 30 months of age to improve meat quality through marbling in Korea. Chung et al. (2000) reported that lipogenesis in subcutaneous fat and intermuscular fat was higher at 30 months of age than at 24 months of age in Hanwoo bulls. Therefore, fattening period is an important factor to produce desirable beef with high proportion of marbling fat in Korea. However, it is difficult for farmers to keep their cattle until 30 months age due to increasing feed expense in recent times.
Thus, the present study was designed to investigate effects of three different fattening periods (i.e. 25, 27 and 29 months) on the growth performance, meat quality and lipogenic gene expression in relation to intramuscular fat content in Hanwoo steers.
MATERIALS AND METHODS
Animals and diets
Forty five Hanwoo calves, 3 months of age and weighing an average of 94.2 [+ or -] 6.4 kg, were distributed into 3 groups of 15 calves, each with an individual feeding system (Calan system, Seil Tech, Korea). The calves were assigned to 3 different fattening periods, which lasted for 25 months (25 mo), 27 months (27 mo) and 29 months (29 mo), respectively. In the treatments, 3 pens (5.3x10.6 m) which had concrete floors with sawdust bedding were arranged with 15 calves per pen. The growth trial was conducted with the animals at 4 months of age after a one-month adaptation period. The present study was categorized into the following periods: early growing (4-6 months age), late growing (7-12 months age), early fattening (13-17, 18 and 20 months age in 25 mo, 27 mo and 29 mo, respectively), and late fattening (18-25 months age, 19-27 months age and 21-29 months age in 25 mo, 27 mo and 29 mo, respectively). During the growing and early fattening periods, animals were offered a commercial concentrate at 1.6%-1.8% of body weight, and they were offered concentrate ad libitum during the late fattening period until slaughtered. During the early and late growing periods, animals were offered commercial concentrate at 1.5-1.6% of body weight. In the early fattening period, animals were offered commercial concentrate at 1.7-1.8% of body weight. The ingredients and chemical composition of the experimental concentrate offered at different growth stages are presented in Table 1. Klein grass hay (Fanicium Contoratom L.) was offered from 2 kg/animal/d (1.8% of BW) to 4 kg/animal/d (1.3% of BW) between 4 and 12 months of age. From 13 to 15 months of age, the hay was changed to rice straw, and animals were offered hay at 2.0, 1.5 and 1.0 kg/animal/d, and rice straw at 2.0, 2.1, and 2.2 kg/animal/d at 13, 14 and 15 months of age, respectively. From 16 months of age only rice straw was offered at 2.2 kg/animal/d. The amount of rice straw offered was decreased with increasing age, and it was restricted to 0.6 kg/d during the late fattening period. The contents of DM, crude protein, ether extract, NDF and ADF of hay were 91.5%, 10.9%, 1.7%, 71.6% and 39.5%, respectively. The contents of DM, crude protein, ether extract, NDF and ADF of rice straw were 88.9%, 3.9%, 1.3%, 60.2% and 39.2%, respectively. Steers had free access to fresh water and mineral block during the whole period. Steers were weighed every month during the experiment period. Forage was fed at 09:00 h daily, and the concentrates in two equal portions at 08:00 and 16:00 h. Dietary refusals were collected and weighed every day. Feed conversion ratio was expressed as average feed intake per daily body weight gain.
Slaughter and carcass assessment
Marbling score was predicted between the 13th thoracic and 1st lumbar vertebrae of steers using ultra sound scanning equipment (Falco 100, 3.5 MHz, 18 cm linear probe, Pie Medical, Netherlands) at 18, 20, 22, 24, 25, 27 and 29 months of age before they were slaughtered, The steers on 25 mo, 27 mo, and 29 mo treatments were slaughtered at 25, 27 and 29 months of age, respectively, according to the procedure of APGS (2007). Their carcass characteristics such as yield grade and quality grade were assessed at 24 h post-mortem by a carcass grader of the Animal Products Grading Service (APGS, 2007), Korea.
Quality grades (marbling, meat color, fat color, texture, and overall mature score) and yield grades (cold carcass, fat thickness, and Longissimus muscle area) were recorded. Live weights were determined immediately before slaughter. After a 24-h chill, cold carcass weights were measured and then the left side of each carcass was cut between the last rib and the first lumbar vertebrae to determine quality grade. The quality grade was determined by assessing the degree of marbling and firmness in the cut surface of the rib eye, in relation to the maturity and fat color of the carcass. The rib eye area was measured from Longissimus muscle taken at the 13th rib and back fat thickness was also measured at the 13th rib. Yield index was calculated as follows: Yield index: 68.184-(0.625xback fat thickness (mm))+(0.130x Longissimus muscle area ([cm.sup.2]))-(0.024xdressed weight (kg)). The degree of marbling was evaluated with the Korean Beef Marbling Standard, and the scores of meat color and fat color were made using the color standard (APGS, 2007). The scores for texture and maturity were made using the APGS reference index (APGS, 2007). The grading ranges were 1 to 9 for marbling score with higher numbers for better quality (1 = devoid, 9 = abundant); meat color (1 = brightly cherry red, 7 = extremely dark red); fat color (1 = white, 7 = dark yellow); texture (1 = soft, 3 = firm); Maturity (1 = youthful, 9 = mature). The Longissimus muscles were taken and immediately frozen in liquid nitrogen, and stored at -80[degrees]C until analysis of lipogenic gene expression.
Quantitative real-time RT-PCR
Total RNA was prepared from each Longissimus muscle (100 mg) using 1 ml of TRIzol reagent (Invitrogene Life Technologies, USA) according to the manufacturer's instructions. For real-time RT-PCR analysis, 500 ng of RNA was reverse transcribed in a 20-[micro]l reaction volume using a RT-PCR high kit (Toyobo, Japan) as described in the RTPCR high kit protocol. We analyzed the expression levels of lipogenic-related genes such as adipocyte fatty acid binding protein 4 (FABP4), glucose transporter 4 (GLUT4), lipoprotein lipase (LPL), acetyl-CoA carboxylase (ACC), stearoyl-CoA desaturase (SCD) and ATP citrate lyase (ACL) in Hanwoo Longissmus muscle. The primer sets were designed with the Primer3 out program, and the sequences of the sets are shown in Table 2. The amplification was performed in a total volume of 50 [micro]l included 5 [micro]l cDNA (125 ng), each 1 [micro]l of 10 pmol/[micro]l forward and reverse primer, 25 [micro]l SYBR Green Master Mix (Toyobo, Japan), and 18 [micro]l distilled water using a 7500 Real time PCR system (Applied Biosystems, USA) as follows: 50[degrees]C for 2 min, 95[degrees]C for 1 min, and 40 cycles of 95[degrees]C for 15 s, 58[degrees]C for 15 s, and 72[degrees]C for 32 s. Following amplification, a melting curve analysis was performed to verify the specificity of the reactions. The end point used in the real-time RT-PCR quantification, [C.sub.T], was defined as the PCR threshold cycle number. All samples were examined for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an internal control, and quantities of each gene were presented as the [2.sup.-[DELTA]Ct] which was calculated using the [DELTA]Ct value (Ct value of sample-Ct value of GAPDH).
Statistical analysis for all dependent variables by treatments as environmental effects was performed using the GLM procedure (Version 8.1; SAS Inst. Inc., Cary, NC). Significant differences among treatments were determined by Duncan's multiple range test (Duncan, 1955) at a level of p<0.05.
RESULTS AND DISCUSSION
Final body weight of steers on 25 mo was lower (p<0.05) compared with 27 mo or 29 mo treatments (Table 3). During the growing and early fattening periods, daily body weight gain was not significantly different among the treatments. Daily body weight gain during the late fattening period and for the overall experimental period was in the order 25 mo>27 mo>29 mo (p<0.05). Total DM intake by steers was higher (p<0.05) on 25 mo compared with 27 mo or 29 mo during the early growing period. However, during the late growing period, total DM intake was higher (p<0.05) in steers on 29 mo compared with 25 mo or 27 mo. During the early fattening period, concentrate intake was higher (p<0.05) and hay intake was lower (p<0.05) in steers on 29 mo compared with 25 mo or 27 mo. Total DM intake was greater in steers on 29 mo followed by 27 mo and 25 mo during the early fattening period (p<0.05). Although concentrate intake was not significantly different among the treatments, rice straw and total feed intakes were higher (p<0.05) in 29 mo compared with other treatments during the late fattening period. During the overall period, concentrate and total DM intakes were higher in 29 mo (p<0.05), while rice straw/hay intake was higher in 25 mo compared with other treatments (p<0.05) during the overall period. Chu et al. (2003) reported similar results that concentrate and rice straw intakes during growing, early and late fattening, and the whole period were 2.60, 5.14, 8.07 and 5.02 kg/d, and 2.87, 2.91, 2.41 and 2.58 kg/d, respectively. In the present study, during the growing period average daily body weight gain and daily feed intake was 0.8 kg/d and 5.4 kg/d/animal, respectively. Similarly, Cho et al. (2001) reported that daily body weight and feed intake of Hanwoo steers during the growing period was 0.7 kg/d and 5.5 kg/d/animal, respectively. Feed conversion ratio in steers fattened for different periods was similar during the early growing period, however, it was lower for the late growing period (p<0.05) in the order 27 mo>25 mo>29 mo. During the whole fattening period, feed conversion was lower in 25 mo compared with 27 mo or 29 mo (p<0.05). In addition, feed conversion ratio was lower (p<0.05) in 25 mo or 27 mo compared with 29 mo during the whole period. Thus, 25 mo and 27 mo had a beneficially lower feed conversion ratio compared with 29 mo.
Slaughter and carcass weights of steers were increased as fattening period was increased (p<0.05) (Table 4). In carcass yield traits, rib-eye area was similar in steers on different treatments, whereas back fat thickness was significantly higher and yield index was lower in 29 mo compared with 25 mo or 27 mo (p<0.05). In the yield grade of 25 mo, appearances of A, B, and C grades were 60%, 40%, and 0%, respectively. The A, B and C grades in 27 mo were 53%, 47%, and 0%, respectively, whereas the A, B and C grades in 29 mo were 33%, 47%, and 20%, respectively. Similar to the present results, Kim et al. (2005) reported that quantity grade A was decreased with increasing age of Hanwoo steers. In quality traits, marbling score, fat color, texture, and maturity were similar in steers on different treatments. Meat color was beneficially lower in the order 27 mo<25 mo<29 mo (p<0.05). The numerically higher marbling score with increasing fattening period could be related to the higher back fat thickness as fattening period increased. Nade et al. (2003) reported that the deposition of body fat such as back fat might be influenced by the feed intake. Yang and Ahn (2001) reported that back fat thickness and marbling score were increased as carcass weight was increased in Hanwoo steers. Thus, marbling score and back fat thickness could be activated as increasing fattening period with feed intake.
The appearance of [1.sup.++], [1.sup.+] and 1 quality grade of beef was 87%, 87%, and 93% in 25 mo, 27 mo and 29 mo, respectively. The best [1.sup.++] grade appearance was 7%, 13%, and 7% in 25 mo, 27 mo and 29 mo, respectively. The present results for meat quality were supported by the marbling score by ultra sound scanning examination which was significantly and numerically higher in 25 mo compared with 27 mo or 29 mo (Table 5). Therefore, intramuscular fat may have been deposited from 25 months of age and consistently lasted until steers were 29 months of age.
Expression levels of lipogenic-related genes
Lipogenesis encompasses the processes of fatty acid synthesis and subsequent triglyceride synthesis and takes place in both liver and adipose tissue, and various enzymes take part in this metabolism (Kersten, 2001). According to Lee et al. (2006) lipogenic-related genes were significantly increased in 27 months-old compared to 12 months-old Hanwoo steers. These reports suggested that expression of lipogenic-related genes may be related with overall fat deposition in cattle. Among these genes, SCD is the major enzyme responsible for conversion of saturated fatty acids into monounsaturated fatty acids in mammalian tissues. Previous reports showed that SCD was a good indicator for intramuscular fat content and fatty acid composition (Taniguchi et al., 2004; Wang et al., 2008). In the present study, the expression level of the SCD gene was significantly increased during the late fattening stages (p<0.01, Figure 1). In addition, mRNA of ACC, ACL and GLUT4 gene were highly expressed in 29 mo compared with 25 mo and 27 mo (p<0.05). However, FABP4 and LPL genes were not significantly different among the treatments.
[FIGURE 1 OMITTED]
Although expression of lipogenic-related genes may differ from various body fat pads or organs, unlike the present results lipogenesis in subcutaneous fat and intramuscular fat was higher in 30 months-old Hanwoo bulls compared with those at 24 months of age (Chung et al., 2000). Smith and Crouse (1984) reported that expression level of the ACL gene increased with increasing age of AngusxHereford crossbred steers. However, the age of these animals, which ranged between 9 and 18 months, was different from that of the present experimental animals. Cattle undergo a dramatic increase in intramuscular fat during a late-finishing stage that occurs between 18 and 26 months of age. In Wagyu (Japanese Black) cattle, approximately 20% of the total intramuscular fat is added between 12 and 26 months (Nishimura et al., 1999) rather than after 27 months of age. In the present study, expression levels of lipogenic-related genes were little different in essence, although the patterns of gene expression were different among the muscle samples. Thus, these findings indicate that rate of lipogenesis made slow progress after the age of 27 months in Hanwoo cattle.
The present findings indicated that different fattening periods resulted in similar carcass characteristics and marbling score in Hanwoo steers. In addition, expression of several lipogenic genes in the Longissmus muscle varied with different fattening periods without consistent effects. Therefore, the present results indicated that different fattening period has no major effect on carcass characteristics, although 25 mo had lower carcass weight compared with 27 mo or 29 mo.
This research was conducted with financial support from Korean National Institute of Animal Science, Rural Development Administration.
Received April 27, 2009; Accepted June 15, 2009
Animal Products Grading Service (APGS). 2007. Grade Rule for Cattle Carcass in Korea. Available: http://www.kormeat.co.kr/01-intro/13-2.asp
Cho, W. M., B. H. Paek, S. W. Kang, J. S. Kim and Y. K. Kim. 2001. Effects of dietary supplements of clay minerals on the growth performance and immunity in growing Hanwoo steers. Kor. J. Anim. Sci. Technol. 43:203-210.
Chu, G. M., H. J. Lee, J. S. Park, H. W. Cho and B. H. Ahn. 2003. Effect of Garlic stalk silage on performance and carcass characteristics of Hanwoo steers. Kor. J. Anim. Sci. Technol. 45:1007-1018.
Chung, C. S., N. S. Kim, M. K. Song, Y. I. Choi, Y. S. Woon, J. K. Chung and J. G. Kim. 2000. Effects of age and feeding level of concentrates on adipose tissue lipogenesis and adipocytes size in Hanwoo bulls. Kor. J. Anim. Sci. Technol. 42:459-466.
Duncan, D. B. 1955. Multiple range and multiple F tests. Biometrics 11:1-42.
Kersten, S. 2001. Mechanisms of nutritional and hormonal regulation of lipogenesis. EMBO reports, 2:282-286.
Kim, K. H., J. H. Lee, Y. G. Oh, S. W. Kang, S. C. Lee, W. Y. Park and K. D. Ko. 2005. The optimal TDN levels of concentrates and slaughter age in Hanwoo steers. Kor. J. Anim. Sci. Technol. 47(5):731-744.
Lee, D. H. 2004. Methods of genetic parameter estimations of carcass weight, longissimus muscle area and marbling score in Korean cattle. Kor. J. Anim. Sci. Technol. 46:509-516.
Lee, D. H., I. Misztal and J. K. Bertrand. 2001. Bayesian analysis of carcass traits using multivariate threshold animal models and Gibbs sampling with missing records in Korean cattle. Kor. J. Anim. Sci. Technol. 43:9-22.
Lee, S. H., E. W. Park, Y. M. Cho, K. H. Kim, Y. K. Oh, J. H. Lee, C. S. Lee, S. J. Oh and D. H. Yoon. 2006. Lipogenesis gene expression profiling in longissimus dorsi on the early and late fattening stage of Hanwoo. Kor. J. Anim. Sci. Technol. 48: 345-352.
Lee, S.-H., E.-W. Park, Y. M. Cho, S.-K. Kim, J.-H. Lee, J.-T. Jeon, C.-S. Lee, S.-K. Im, S.-J. Oh, J. M. Thompson and D. Yoon. 2007. Identification of differentially expressed genes related to intramuscular fat development in the early and late fattening stages of Hanwoo steers. J. Biochem. Mol. Biol. 40:757-764.
Nade, T., S. Hirabara, T. Okumura and K. Fujita. 2003. Effects of vitamin A on carcass composition concerning younger steer fattening of Wagyu cattle. Asian-Aust. J. Anim. Sci. 16(3): 353-358.
Nishimura, T., A. Hattori and K. Takahashi. 1999. Structural changes in intramuscular connective tissue during the fattening of Japanese black cattle: effect of marbling on beef tenderization. J Anim. Sci. 77:93-104.
SAS Institute Inc. 1999. SAS/STAT user's guide. Version 8, (4th ed.). Cary, NC, USA.
Smith, D. B. and J. D. Crouse. 1984. Relative contributions of acetate, lactate and glucose to lipogenesis in bovine intramuscular and subcutaneous adipose tissue. J. Nutr. 14: 792-800.
Taniguchi, M., H. Mannen, K. Oyama, Y. Shimakura, A. Oka, H. Wantanabe, T. Kojima, M. Komatsu, G. S. Harper and S. Tsuji. 2004. Differences in stearoyl-CoA desaturase mRNA levels between Japanese black and Holstein cattle. Livest. Prod. Sci. 87:215-220.
Tatum, J. D., G. C. Smith and Z. L. Carpenter. 1982. Interrelationships between marbling, subcutaneous fat thickness, and cooked beef palatability. J. Anim. Sci. 34:777-784.
Wang, Y. H., N. I. Bower, A. Reverter, S. H. Tan, N. De Jager, R. Wang, S. M. McWilliam, L. M. Cafe, P. L. Greenwood and S. A. Lehnert. 2008. Gene expressions patterns during intramuscular fat development in cattle. J. Anim. Sci. 87:199-130.
Yang, S. J. and B. H. Ahn. 2001. Effects of vitamin A and E concentration in the blood on carcass characteristics of Hanwoo steers. Kor. J. Anim. Sci. Technol. 43:895-904.
Eung Gi Kwon (a), Byung Ki Park (a), Hyeong Cheol Kim, Young Moo Cho, Tae II Kim, Sun Sik Chang, Young Kyoon Oh, Nam Kuk Kim, Jun Ho Kim (1), Young Jun Kim (1), Eun-Jib Kim (2), Seok Ki Im and Nag-Jin Choi (2), *
National Institute of Animal Science, Pyongchang, Gangwon, 232-952, Korea
* Corresponding Author: Nag-Jin Choi. Tel: +82-41-580-1064, Fax: +82-41-580-1241, E-mail: firstname.lastname@example.org
(1) Department of Food and Biotechnology, Korea University, Jochiwon, Chungnam, 339-700, Korea.
(2) Division of Animal Husbandry, Cheonan Yonnam College, Cheonan, Chungnam, 330-802, Korea.
(a) The first two authors equally contributed to this work.
Table 1. Ingredient and chemical composition of the experimental diets Early Late Early Late growing growing fattening fattening Item period period period period Ingredient (DM) Corn 22.00 25.60 36.31 -- Corn, flaked -- -- -- 45.00 Barley 13.00 11.00 10.00 10.00 Soybean meal 5.00 -- -- -- Soy-hull -- -- -- 15.00 Rape seed meal 10.00 6.00 5.70 -- Gluten feed 20.00 26.06 20.00 15.00 Wheat bran 25.00 26.00 23.00 7.99 Limestone 1.00 1.00 1.00 0.63 Lasalocid 0.02 0.02 0.02 0.02 Vit.-min. premix (1) 0.20 0.30 0.20 0.20 Salt 0.30 0.30 0.30 0.30 Molasses 3.00 3.00 3.00 3.00 Calcium phosphate 0.48 0.72 0.47 0.46 Sodium bicarbonate -- -- -- 0.40 Yeast culture -- -- -- 2.00 Total 100.00 100.00 100.00 100.00 Chemical composition (%) Dry matter 85.79 88.40 86.08 86.06 Crude protein 16.63 14.77 13.16 10.95 Ether extract 2.20 2.71 2.89 3.21 Crude fiber 7.36 7.09 6.13 7.06 Crude ash 5.40 6.53 4.91 3.66 Neutral detergent fiber 31.14 29.52 22.85 19.50 Acid detergent fiber 4.06 5.21 2.74 7.70 Ca 0.46 1.03 0.81 0.76 P 0.65 0.76 0.69 0.46 (1) Contains the following, (Vit. A, 2,650,000 IU; Vit. D3, 530,000 IU; Vit. E, 1,050 IU; BHT (butylated hydroxy toluene), 10,000 mg; Fe, 13,200 mg; Mn, 4,400 mg; Cu, 2,200 mg; co, 440 mg; I, 440 mg)/kg. Table 2. Primer sequences of lipogenesis genes for real-time PCR analysis Primer name Primer sequence (5'-3') FABP4 (1) Forward CGT GGG CTT TGC TAC CAG Reverse TGG TTG ATT TTC CAT CCC AG GLUT4 (2) Forward GGT GGC ATG ATC TCA TCC TT Reverse AGGAGG AGT GGC CAT AAG GT ACC (3) Forward ATG GTC TTT GCC AAC TGG AG Reverse TGA TTT CGA CTG TCC CTT CC LPL (4) Forward TAC CCT GCC TGA AGT TTC CAC Reverse CCC AGT TTC AGC CAG ACT TTC ACL (5) Forward CAG GAC ACT GCA GGA GTC AA Reverse CAA ACA CTC CAG CCT CCT TC SCD (6) Forward CCA GAG GAG GTA CTA CAA ACC TG Reverse AGC CAG GTG ACG TTG AGC GAPDH (7) Forward GGGTCATCATCTCTGCACCT Reverse GGTCATAAGTCCCTCCACGA Primer name GenBank accession No. FABP4 (1) NM 174314 GLUT4 (2) AY 458600 ACC (3) NM 174224 LPL (4) XM 871618 ACL (5) BC 108138 SCD (6) NM 173959 GAPDH (7) BC102589 (1) FABP4 = Adipocyte fatty acid binding protein 4. (2) GLUT4 = Glucose transporter 4. (3) ACC = Acetyl-CoA carboxylase. (4) LPL = Lipoprotein lipase. (5) ACL = ATP citrate lyase. (6) SCD = Stearoyl-CoA desaturase. (7) GAPDH = Glyceraldehyde-3-phosphate dehydrogenase. Table 3. Effect of fattening period on intake, body weight (BW) gain and feed conversion ratio of Hanwoo steers Item Treatment 25 mo (1) 27 mo (2) 29 mo (3) Initial BW (kg) 112.5 110.8 111.7 Final body weight (kg) 637.7 (b) 668.3 (a) 695.8 (a) Daily BW gain (kg/d) Early growing period 0.76 0.65 0.67 Late growing period 0.83 0.90 0.86 Early fattening period 0.90 0.89 0.84 Late fattening period 0.80 (a) 0.76 (ab) 0.69 (b) Overall period 0.83 (a) 0.80 (ab) 0.77 (b) Intake (DM kg/d) Early growing period Concentrate 1.72 1.72 1.72 Hay 2.02 1.99 1.98 Total feed 3.73 (a) 3.70 (b) 3.70 (b) Late growing period Concentrate 3.45 3.46 3.46 Hay/rice straw 3.52 3.49 3.52 Total feed 6.96 (b) 6.95 (b) 7.41 (a) Early fattening period Concentrate 5.93 (c) 6.39 (b) 6.64 (a) Hay 2.56 (a) 2.42 (b) 2.36c Total feed 8.71 (c) 8.81 (b) 9.00 (a) Late fattening period Concentrate 8.94 8.62 9.11 Rice straw 0.70 (c) 0.72 (b) 0.82 (a) Total feed 9.64 (ab) 9.35 (b) 9.93 (a) Whole period Concentrate 5.01 (b) 5.05 (b) 5.23 (a) Hay/rice straw 2.20 (a) 2.16 (b) 2.17 (b) Total feed 7.26 (b) 7.20 (b) 7.51 (a) Feed conversion ratio Early growing period 5.32 5.82 6.67 Late growing period 9.08 (ab) 8.37 (b) 9.35 (a) Early fattening period 9.94 (b) 10.44 (b) 11.41 (a) Late fattening period 13.41 (b) 15.63 (a) 16.43 (a) Whole period 9.44 (b) 10.06 (b) 10.96 (a) Item SEM p value Initial BW (kg) 1.1824 0.8526 Final body weight (kg) 6.8278 0.0012 Daily BW gain (kg/d) Early growing period 0.0252 0.1537 Late growing period 0.0133 0.1137 Early fattening period 0.0145 0.2140 Late fattening period 0.0172 0.0229 Overall period 0.0093 0.0292 Intake (DM kg/d) Early growing period Concentrate -- -- Hay 0.0062 0.0540 Total feed 0.0062 0.0346 Late growing period Concentrate 0.0047 0.5565 Hay/rice straw 0.0162 0.7063 Total feed 0.0602 0.0009 Early fattening period Concentrate 0.0450 <0.0001 Hay 0.0129 <0.0001 Total feed 0.0196 <0.0001 Late fattening period Concentrate 0.0896 0.0796 Rice straw 0.0079 <0.0001 Total feed 0.0915 0.0306 Whole period Concentrate 0.0266 0.0005 Hay/rice straw 0.0053 0.0010 Total feed 0.0323 <0.0001 Feed conversion ratio Early growing period 0.3281 0.2394 Late growing period 0.1646 0.0393 Early fattening period 0.1982 0.0062 Late fattening period 0.4070 0.0048 Whole period 0.1612 0.0001 (a b, c) In this and all other tables, means with different superscripts in the same row significantly differ (p<0.05). (1, 2, 3) In this and all other tables, 25 mo = slaughtered at 25 months age, 27 mo = slaughtered at 27 months age, 29 mo = slaughtered at 29 months age. Table 4. Effect of fattening period on carcass characteristics of Hanwoo steers Parameters Treatments 25 mo 27 mo 29 mo Slaughter weight (kg) 637.7 (b) 668.3 (a) 695.8 (a) Carcass weight (kg) 382.7 (b) 406.2 (a) 424.5 (a) Yield traits (1) Rib-eye area ([cm.sup.2]) 87.40 86.53 87.00 Back fat thickness (mm) 9.33 (b) 9.60 (b) 13.13 (a) Yield index (%) 67.75 (a) 66.91 (a) 64.34 (b) Yield grade (2) (A:B:C, head) 9:6:0 8:7:0 5:7:3 Quality traits (3) Marbling score 5.80 5.93 6.07 Meat color 4.53 (ab) 4.33 (b) 4.80 (a) Fat color 3.00 3.00 3.00 Texture 1.13 1.33 1.33 Maturity 2.13 2.20 2.47 Quality grade 1:8:4:2 2:5:6:2 1:7:6:1 ([1.sup.++]:[1.sup.+]: 1:2, head) Parameters SEM p value Slaughter weight (kg) 6.90 0.0055 Carcass weight (kg) 4.98 0.0014 Yield traits (1) Rib-eye area ([cm.sup.2]) 0.87 0.9230 Back fat thickness (mm) 0.64 0.0222 Yield index (%) 0.50 0.0108 Yield grade (2) (A:B:C, head) -- -- Quality traits (3) Marbling score 0.24 0.9053 Meat color 0.07 0.0346 Fat color -- -- Texture 0.07 0.3765 Maturity 0.07 0.0949 Quality grade -- -- ([1.sup.++]:[1.sup.+]: 1:2, head) (1) Area was measured from Longissimus muscle taken at 13th rib and back fat thickness was also measured at 13th rib; Yield index were calculated using the following equation: Yield index: 68.184-(0.625xback fat thickness (mm))+(0.130x Longissimus muscle area ([cm.sup.2]))-(0.024xdressed weight (kg)). (2) Carcass yield grades from C (low yield) to A (high yield). (3) Grading ranges are 1 to 9 for marbling score with higher numbers for better quality (1 = devoid, 9 = abundant); meat color (1 = brightly cherry red, 7 = extremely dark red); fat color (1 = white, 7 = dark yellow); texture (1 = soft, 3 = firm); Maturity (1 = youthful, 9 = mature); quality grades from 3 (low quality) to [1.sup.++] (very high quality). Table 5. Marbling score measured using ultra sound scanning at different growth stages Age (month) Treatment SEM p value 25 mo 27 mo 29 mo 18 1.47 1.13 1.13 0.07 0.09 20 2.47 (a) 1.87 (b) 2.07 (ab) 0.10 0.04 22 3.20 2.73 2.80 0.12 0.25 24 5.00 (a) 3.87 (b) 3.87 (b) 0.18 0.01 25 5.73 (a) 4.80 (ab) 4.53 (b) 0.21 0.05 27 -- 5.80 5.40 0.26 0.45 29 -- -- 6.07 0.38 -- (1) Grading ranges are 1 to 9 for marbling score with higher numbers for better quality.
|Gale Copyright:||Copyright 2009 Gale, Cengage Learning. All rights reserved.|