Cytotoxic activity on human cancer from Ruta graveolens.
|Article Type:||Brief article|
(Dosage and administration)
Rue (Health aspects)
|Publication:||Name: Australian Journal of Medical Herbalism Publisher: National Herbalists Association of Australia Audience: Academic Format: Magazine/Journal Subject: Health Copyright: COPYRIGHT 2007 National Herbalists Association of Australia ISSN: 1033-8330|
|Issue:||Date: Spring, 2007 Source Volume: 19 Source Issue: 1|
|Topic:||Event Code: 310 Science & research|
|Geographic:||Geographic Scope: Australia Geographic Code: 8AUST Australia|
Rethy B, Zupko I, Minorics R, Hohmann J, Ocsovszki Iet al. 2007.
Investigation of Cytotoxic Activity on Human Cancer Cell Lines of
Arborinine and Furanoacridones Isolated from Ruta graveolens. Planta Med
Nine acridone alkaloids (1: arborinine, 2: evoxanthine, 3: isogravacridone chlorine, 4: rutacridone, 5: gravacridonedio, 6: gravacridonetriol, 7: gravacridonediol monomethyl ether, 8: gravacridonetriol monoglycoside and 9: gravakidonediol monoglycoside) isolated from Rum graveolens were tested for their cytotoxic activity on human cancer cells.
The compounds were applied in vitro to three types of human cancer cells: MCF-7 (breast adenocarcinoma), HeLa (cervix adenocarcinoma) and A431 (skin epidermoid carcinoma). Doxorubicin and cisplatin were used as positive controls. Arborine displayed the highest antiproliferative effects. Compounds 3, 4, 5 and 7 also displayed significant antiproliferative effects.
The effects of compounds 3, 4 and 7 were examined more closely. Staining of the cell cultures revealed that the application of the compounds to HeLa cells resulted in typical morphological features of apoptosis, including increased cell membrane permeability, cellular shrinkage and granulation in the nucleus.
When cell cultures were examined for the proportion of apoptotic cells, they were found to increase in a dose dependant manner. Compounds 3 and 7 increased apoptosis most significantly. The mRNA ratio of Bax/Bcl-2 (markers of apoptosis) was also dose dependently increased.
Researchers propose the use of these acridone alkaloids as starting structures for the development of novel anticancer agents.
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|Gale Copyright:||Copyright 2007 Gale, Cengage Learning. All rights reserved.|