Comparison of HTLV-associated myelopathy (HAM) in HIV-positive and HIV-negative patients at a tertiary South African hospital.
Abstract: Background. HTLV-1 associated myelopathy (HAM), or tropical spastic paraparesis, is caused by a retrovirus, the human T-cell lymphotropic virus (HTLV). Although patients with HAM and HIV infection have been described, to our knowledge no direct comparison has been made between patients who are HIV positive and suffering from HAM (HHAM) v. those who are HIV negative and suffering from HAM.

Aim. We aimed to compare clinical and radiological findings in HIV-positive and -negative patients with HAM.

Methods. Adult patients who presented to the Neurology Unit at the Steve Biko Academic Hospital from May 2005 to June 2012 with a progressive myelopathy and HTLV seropositivity were retrospectively identified and their clinical and radiological data were collected and reviewed.

Results. 21 patients with HAM were identified, of whom 9 were HIV-positive and 11 HIV-negative. One patient, whose HIV status had not been established, was not included in the study. Although the trend did not reach statistical significance, co-infected patients tended to present at an earlier age (HHAM 6/9 (66%) <40 years old; HAM 2/11 (18%) <40 years old) and presented to hospital earlier (HHAM 6/9 (66%) < 3 years symptomatic; HAM 7/11 (63%) > 3 years symptomatic). Cord atrophy occurred in 7/8 dually infected patients and 8/10 HIV-negative patients.

Conclusion. Although the study is limited by the small number of patients, co-infected patients tended to have a younger age of onset and to present to hospital sooner, and thoracic cord atrophy was very common.

S Afr Med J 2013;103(1):43-46. DOI:10.7196/SAMJ.5298
Authors: Schutte, C.M.
Townsend, T.
Van Coller, R.
Olorunju, S.
Pub Date: 01/01/2013
Publication: Name: South African Medical Journal Publisher: South African Medical Association Audience: Academic Format: Magazine/Journal Subject: Health Copyright: COPYRIGHT 2013 South African Medical Association ISSN: 0256-9574
Issue: Date: Jan, 2013 Source Volume: 103 Source Issue: 1
Accession Number: 312828772
Full Text: Human T-cell lymphotropic virus type-1 (HTLV-1) is a type C retrovirus endemic to tropical areas. Most infected people are asymptomatic carriers, but an estimated 0.25%--2% of carriers develop a progressive myelopathy, known as HTLV-associated myelopathy (HAM) or tropical spastic paraparesis (TSP). (1,2) HTLV-2 is more common among intravenous drug abusers, and may present with a clinical picture similar to HAM. (2)

During the last two decades, the HIV pandemic has influenced the spectrum of neurological disorders markedly--up to 40% of patients with HIV infection clinically show neurological disorders, (3) and neuropathological abnormalities are even more common. Co-infections of HIV and HTLV-1 have been described (4,5) and in South Africa a high prevalence of HAM has been found in KwaZulu-Natal, (5) with up to 36% of patients showing HIV co-infection. A study from Brazil reported on patients with HIV and HAM4 but did not directly compare patients with and without HIV infection.

We aimed to evaluate the number of patients with HAM seen at the Neurology Department of the Steve Biko Academic Hospital, a tertiary hospital in Gauteng Province, and to compare the clinical and radiological findings of patients who were co-infected with HIV with those who were HIV negative.

Patients and methods

Patients were identified retrospectively from a list of patients with positive HTLV-1 serology who presented to the National Health Laboratory Services (NHLS) at the Steve Biko Academic Hospital, from May 2005 to June 2012. To ascertain which of these seropositive patients had presented to the hospital's Department of Neurology, the department's electronic database was consulted. Patients included in the study were those 13 years and older, with signs and/or symptoms indicative of possible spinal cord pathology, who had an HIV test done during investigation. Patients excluded were those who had not undergone an HIV test, or who had another recognised aetiology that could better account for symptoms and signs.

The study was approved by the Ethics Committee of the University of Pretoria.

The serology for HTLV-1 detection was performed with ELISA by the NHLS. The spinal cord diameters were measured at level T7 on MRI by a blinded observer who had no access to the patients' clinical information. The data was analysed to determine summary statistics and proportions, and the proportions were compared using an independent t-test. Meanwhile, comparison for continuous data was undertaken using t-tests for independent samples. Stata 12 was the software used.

Results

The records showed 73 patients had positive serology for HTLV-1, of whom 25 were seen at the Department of Neurology with signs of a myelopathy. Of these, 20 were included in the study: 9 tested positive for HIV, and 11 tested negative. Of the 5 patients who were excluded, 4 had alternative aetiologies for their suspected myelopathy: 1 (HIV-negative) was found to have a profound vitamin B12 deficiency, and 3 had spinal tuberculosis. The 5th patient had HAM but had not had not undergone an HIV test.

The clinical data of the patients are presented in Table 1 (HIV-negative patients with HAM) and Table 2 (HIV-positive patients with HAM).

The HIV-negative group consisted of 8 men and 3 women between 21 and 57 years of age, with a mean of 43,7 [+ or -] 8,61 years. Minimum duration of symptoms was 4 months and maximum 14 years; most patients (7/11) had been symptomatic for 3 years or more. Sphincter disturbances were common, occurring in 8/11 patients (72%), and sensory complaints were noted in 9/10. Radiological reports noted atrophy of the cord on MRI in 6 patients, swelling of the cord in one and evidence of possible transverse myelitis in one. A blinded observer measured cord diameter at level T7, yielding values ranging from 3.8 mm to 9.6 mm (normal: 6.5 mm--9.5 mm), with a mean of 5.4 mm. Values <5 mm, implying severe atrophy, were measured in 60% of patients.

[FIGURE 1 OMITTED]

Five females and four males were seen in the HIV-positive group. Their ages ranged from 25 to 49 years with a mean of 36.6 [+ or -] 7.06 years, but though this was lower than in the HIV-negative group, no evidence of a statistically significant difference with respect to age could be demonstrated. Symptoms had been present for 3 months to 8 years, with 66% of patients symptomatic for less than 3 years. Sphincter disturbances were present in all patients, and 75% had sensory complaints. A trend towards statistical significance (p<0.055) between patients <40 years old and >40 years old was observed in proportion to sensory symptoms: all older patients had sensory symptoms, compared with half of the younger ones.

Patients' CD 4 counts ranged from 5 to 1 076 x 106/l, while 56% had counts of less than 350 x 106/l. Two patients were on highly active antiretroviral therapy (HAART) at presentation. Atrophy of the cord was reported on MRI studies in 4 patients, and a transverse myelitis picture in 1. When the thoracic cord was measured at level T7, the values ranged from 2.9 mm to 6.7 mm (mean: 4.9 mm). Thus, 7/8 patients had cord atrophy, and values of <5mm were seen in 50% of patients (see Fig. 1). There was no evidence of statistical difference in the proportion of patients with cord atrophy in the HIV-positive v. the HIV-negative group.

Discussion

HTLV-associated myelopathy was first described in the 1950s in the Caribbean,7 but it was only in 1985 that the condition was linked with antibodies to the retrovirus known as HTLV. (8) HAM is a progressive neurodegenerative disease that primarily affects the spinal cord and brain. Risk factors for developing an HTLV-associated myelopathy are the same as those for the transmission of HIV, including younger age of initiation of sexual intercourse and multiple sexual partners. (9)

Patients present with a slowly progressive paraparesis affecting the lower limbs (10) with a cumulative risk of 1.7% of developing HAM by the age of 75 years if infected with the virus. (11) Other clinical features include lumbar pain, detrusor instability, and minor sensory changes, especially paraesthesias and loss of vibration sense. (12) In our study, the patients singly infected with HTLV showed a disease progression and clinical signs and symptoms in keeping with this classic description.

Our study showed that co-infected patients had a more rapid progression of symptoms that led to an earlier hospital visit. The duration of symptoms in more than half of our patients was less than a year--a finding not documented in other studies. The clinical picture in the dually infected patients may reflect virus-virus interaction.13 Dually infected patients have higher levels of HTLV antigen and tax/ rex RNA expression in peripheral blood mononuclear cells, thereby up-regulating HTLV viral message and portending HTLV-associated neurological disease. (14)

Patients in a previous study by Beilke et al. (15) had high CD 4 counts, ranging from 890-1 6 1 3 x 106/l. However, our study shows that HAM can occur at any stage of immune suppression: CD 4 counts ranged from 5 to 1 076 x [10.sup.6]/l, and most of our patients had CD 4 counts of less than 350 x [10.sup.6]/l. It is unlikely that any of the patients had HIV-associated vacuolar myelopathy since the characteristic lesions (hyperintensities in the posterior and lateral columns) were not noted on MRI. About half of all patients with HAM currently seen at the Steve Biko Academic Hospital are infected with HIV, compared with 33% in KwaZulu-Natal. (5) Co-infection of HTLV-1 and HIV in patients with HAM has been mentioned in several studies. (4,5,14,15) According to Berger et al., (16) patients co-infected with HIV/HTLV are at increased risk for developing HAM. Elsewhere, a Brazilian study compared the clinical evidence of a myelopathy in 32 co-infected patients (HIV/ HTLV) with 118 patients singly infected with HIV, and found a higher incidence of signs of myelopathy in co-infected patients. (4)

Bhigjee et al. previously found a co-infection rate of HIV and HTLV-1 in 6%--8% of patients with HAM. In a study on the spectrum of myelopathies in HIV positive patients in South Africa,5 they found that 33% of patients had seropositivity for both HIV and HTLV-1, suggesting a higher frequency of myelopathy in co-infected patients. In addition, the patients were younger than those without HIV infection, but otherwise similar, without profound immunosuppression.

Although the findings did not reach statistical significance, the tendency for HIV-positive patients to be symptomatic from HAM at a younger age is also suggested in our study, where 6/9 co-infected patients were younger than 40 years and only 2/11 patients under the age of 40 were singly infected with HTLV. A retrospective study from Louisiana15 evaluated HTLV 1 and 2 infection in HIV-positive patients, identifying 5 patients with HAM and HTLV-1 infection whose clinical findings were described, but not specifically compared with those of HIV-negative patients with HAM from the same unit. The patients' ages ranged from 27--51 years, with 3 younger than 40 years.

The reported incidence of cord atrophy in HAM varies from 5.6% to 74%. (17-18) Cord swelling may also occur, possibly in the earlier stages of disease. (19) Measurements of the thoracic cord diameter in our patients showed that 80% of the HIV-negative group and 88% from the HIV-positive group showed atrophy of the cord on MRI--more than currently indicated in the literature. In addition to spinal cord abnormalities, white matter lesions in the brain have also been described in individuals with HAM. (20) Only 3 of our 9 co-infected patients underwent brain imaging, but cerebral atrophy occurred in all 3, possibly reflecting the longstanding HIV infection. Only 1 showed white matter involvement.

Conclusion

We found that patients co-infected with HIV and HTLV tended to have a younger age of onset, an earlier presentation to hospital and universal sphincter involvement, and commonly had evidence of spinal cord atrophy. In addition, when compared with other cases with dual infection described in the literature, our patients had a wide range of CD4 counts, indicating that HAM in HIV-positive patients may occur in cases of profound immunosuppression as well as early in the course of infection. The study is limited by the small number of patients assessed, and more data need to be collected to show possible statistical differences.

Accepted 12 September 2012

References

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C-M Schutte, T Townsend, R Van Coller, S Olorunju Department of Neurology, Steve Biko Academic Hospital, Pretoria C-M Schutte, M Med (Neurol), MD T Townsend, M Med (Neurol),FCN R Van Coller, M Med (Neurol)

Medical Research Council, Pretoria S Olorunju, PhD

Corresponding author: C-M Schutte (cschutte@meic.up.ac.za)
Table 1. Clinical data of HIV-negative patients with HAM

                 Age     Duration   Power
Number   Sex   (years)   (months)   (MRC)

1         M      48        144        2
2         M      47         48        3
3         M      44         9         3
4         M      43         4         2
5         F      21         12        4
6         M      47         72        4
7         F      43         36        3
8         M      51        168        1
9         M      57         36        3
10        M      41        156        4
11        F      39         24        3

         Sphincter   Sensory    Cord
Number   problems    symptoms   (mm)

1            Y          N        NA
2            Y          Y       4,4
3            Y          Y       9,6
4            N          NA      8,8
5            N          Y       5,1
6            Y          Y       4,5
7            N          Y       3,8
8            Y          Y       4,0
9            Y          Y       4,2
10           Y          Y       5,4
11           Y          Y       4,0

Power grading is by MRC (6) grading scale out of 5; proximal muscle
groups of the lower limbs are reflected; Y = yes, N = no,
NA = not available.

Table 2. Clinical data of HIV-positive patients with HAM

                 Age     Duration   Power   Sphincter
Number   Sex   (years)   (months)   (MRC)   problems

1         M      34         4         3         Y
2         M      42         6         5         Y
3         M      32         5         4         Y
4         F      36         96        4         Y
5         F      25         11        4         Y
6         M      36         3         3         Y
7         F      45         36        4         Y
8         F      31         24        2         Y
9         F      49         96        2         Y

         Sensory    Cord        CD4
Number   symptoms   (mm)   x[10.sup.6]/l

1           Y        NA          5
2           Y       6,2         27
3           N       6,7         31
4           N       4,3         300
5           NA      4,4         484
6           Y       5,7         148
7           Y       4,1        1076
8           Y       2,9         635
9           Y       5,1         580

Power grading is by MRC (6) grading scale out of 5; proximal muscle
groups of the lower limbs are reflected; Y = yes, N = no,
NA = not available.
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