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Avian Pathol: Detection of multiple strains of
Pasteurella multocida in fowl cholera outbreaks by polymerase chain
reaction-based typing.
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| Article Type: | Reprint |
| Subject: |
Pasteurella infections
(Diagnosis) Chicken cholera (Diagnosis) Polymerase chain reaction (Observations) Veterinary medicine (Research) |
| Authors: |
Shivachandra, S.B. Kumar, A.A. Gautam, R. |
| Pub Date: | 09/01/2006 |
| Publication: | Name: Journal of Avian Medicine and Surgery Publisher: Association of Avian Veterinarians Audience: Academic Format: Magazine/Journal Subject: Health Copyright: COPYRIGHT 2006 Association of Avian Veterinarians ISSN: 1082-6742 |
| Issue: | Date: Sept, 2006 Source Volume: 20 Source Issue: 3 |
| Topic: | Event Code: 310 Science & research |
| Geographic: | Geographic Scope: United States Geographic Code: 1USA United States |
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| Accession Number: | 167430680 |
| Full Text: |
Applicability of molecular methods for the detection and
differentiation of Pasteurella multocida strains involved in 2 separate
fowl cholera outbreaks in a single poultry farm was investigated. A
total of 12 and 18 strains of P multocida obtained from 2 separate
outbreaks were subjected to phenotypic and genotypic characterization.
Phenotypically, all strains were similar; however, DNA-based techniques
employing polymerase chain reaction (PCR) assays were found to be highly
specific and sensitive for rapid detection and differentiation of
strains. All 30 strains gave amplicons of ~460 bp and ~1044 bp specific
for P multocida and capsular serogroup A in the Multiplex Capsular PCR
typing system. Molecular typing techniques such as repetitive extragenic
palindromic PCR, enterobacterial repetitive intergenic consensus PCR,
and single primer PCR differentiated all 30 strains into different
profiles. However, similar patterns of genome fragments were observed
among all strains following restriction endonuclease analysis using the
enzyme HpaII. The current investigation revealed involvement of the same
and multiple strains of P multocida in 2 outbreaks. The results also
indicated that molecular methods of detection and typing are rapid in
comparison with conventional methods for epidemiological investigations
of fowl cholera outbreaks. 2005;34:456-462. |
| Gale Copyright: | Copyright 2006 Gale, Cengage Learning. All rights reserved. |