Avian Dis.: Evaluation of plasma (1 [right arrow] 3) [beta]-D-glucan concentrations in birds naturally and experimentally infected with Aspergillus fumigatus.
Article Type: Reprint
Subject: Aspergillus (Health aspects)
Blood plasma (Properties)
Birds (Diseases)
Birds (Care and treatment)
Birds (Demographic aspects)
Pub Date: 06/01/2012
Publication: Name: Journal of Avian Medicine and Surgery Publisher: Association of Avian Veterinarians Audience: Academic Format: Magazine/Journal Subject: Health Copyright: COPYRIGHT 2012 Association of Avian Veterinarians ISSN: 1082-6742
Issue: Date: June, 2012 Source Volume: 26 Source Issue: 2
Product: Product Code: 2831130 Blood Plasma Products NAICS Code: 325414 Biological Product (except Diagnostic) Manufacturing SIC Code: 2836 Biological products exc. diagnostic
Geographic: Geographic Scope: United States Geographic Code: 1USA United States
Accession Number: 298292712
Full Text: Evaluation of plasma (1 [right arrow] 3) [beta]-D-glncan concentrations in birds naturally and experimentally infected with Aspergillus fumigatus. Burco JD, Ziccardi MH, Clemons KV, et al. Avian Dis. 2012;56:192-199.

Avian aspergillosis, most often caused by Aspergillus fumigatus, is a common and devastating disease affecting a range of bird species. Early diagnosis is difficult and often unreliable. The current study evaluated the use of measuring (1 [right arrow] 3)-[beta]-D-glucan (BG) concentrations in avian plasma samples to aid in the diagnosis of aspergillosis. We evaluated a commercially available BG assay (Fungitell, Beacon Diagnostics) by using 178 plasma samples from naturally infected, experimentally infected, and aspergillosis-free birds. Although there was variation in BG concentration, as reflected by high SDs, seabirds with confirmed aspergillosis had the highest mean BG concentrations (M = 3098.7 pg/dL, SD = 5022.6, n = 22) followed by companion avian species and raptors with confirmed aspergillosis (M = 1033.8 pg/dL, SD = 1531.6, n = 19) and experimentally infected Japanese quail (Coturnix japonica; M = 1066.5 pg/dL, SD = 1348.2, n = 17). Variation in severity of disease, differences among species of birds with and without disease, and also different levels in environmental exposure likely contribute to the differences among avian groups. The overall sensitivity and specificity of the BG test for diagnosis of aspergillosis in birds was 60.0% and 92.7%, respectively, with an overall optimized avian cutoff value of [greater than or equal to] 461 pg/dL for positive disease. Our findings suggest that, although BG concentrations are highly variable between and within different avian groups, it could serve as a useful adjunctive diagnostic test for aspergillosis that is applicable to multiple avian species in some settings, particularly as a negative predictor of infection.

Burco JD, Ziccardi MH, Clemons KV, et al.
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