Antiviral effect of the Lamiaceae family against Herpes simplex.
|Article Type:||Brief article|
Herpes simplex virus
Lamiaceae (Physiological aspects)
Materia medica, Vegetable (Health aspects)
Materia medica, Vegetable (Usage)
Materia medica, Vegetable (Research)
Plant extracts (Health aspects)
Plant extracts (Usage)
Plant extracts (Research)
|Publication:||Name: Australian Journal of Medical Herbalism Publisher: National Herbalists Association of Australia Audience: Academic Format: Magazine/Journal Subject: Health Copyright: COPYRIGHT 2007 National Herbalists Association of Australia ISSN: 1033-8330|
|Issue:||Date: Spring, 2007 Source Volume: 19 Source Issue: 1|
|Topic:||Event Code: 310 Science & research|
|Geographic:||Geographic Scope: Australia Geographic Code: 8AUST Australia|
Nolkemper S, Reichling J, Stintzing F, Carle R, Sehnitzler P. 2006.
Antiviral Effect of Aqueous Extracts from spp of the Lamiaceae Family
against Herpes simplex Virus Type 1 and Type 2 in vitro. P/anta Med
Extracts of Melissa officinalis, Mentha piperita, Rosmarinus officinalis, Salvia officinalis, Thymus vulgaris and Prunella vulgaris were tested to determine their cytotoxic concentrations. Extracts were then used at their maximum noncytotoxic concentrations.
Herpes simplex virus type 1 (HSV-1), HSV-2 and an acyclovir resistant HSV-1 (HSV-1-[ACV.sup.res]) were used to test the antiviral activity of the herbal extracts. In order to elucidate the mode of antiviral action, cells and viruses were incubated together during adsorption; cells were pretreated with extracts before viral infection; viruses were incubated with extracts before cell infection; or after penetration of the virus into the host cell it was treated with extract.
Untreated virus infected cells were used as controls. Acyclovir was used as a positive control. After 72h of incubation the cells were stained and the number of plaques of treated cells and viruses were compared to the untreated controls to calculate the extent of plaque reduction.
Pretreatment of the cells before being exposed to HSV was the most effective at reducing plaque formation. Extracts added during the adsorption period of viruses to host cells was also reasonably effective. Treatment after the penetration of the virus did not significantly reduce plaque formation.
The most active agent was found to be sage (Salvia officinalis), which reduced plaque formation by 85% for HSV-1 and by 95% for HSV-2, after 10 minutes of incubation. After 2 hours of incubation the extracts showed an antiviral activity of 90-98% for HSV-1 and > 98% for HSV-2.
HSV-1-ACVres plaque formation was reduced by 85% by Mentha piperita and 97% by sage.
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