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Ha Sungji - - 2014
ABSTRACT. Stem cell therapy is a promising tool for the treatment of diverse conditions, including neurodegenerative diseases such as Alzheimer's disease (AD). To understand transplanted stem cell biology, in vivo imaging is necessary. Nanomaterial has great potential for in vivo imaging and several noninvasive methods are used, such as magnetic ...
Chang Minhyeok - - 2014
ABSTRACT. We present a single-molecule imaging platform that quantitatively explores the spatiotemporal dynamics of individual insulin receptors in living cells. Modified DNA aptamers that specifically recognize insulin receptors (IRs) with a high affinity were selected through the SELEX process. Using quantum dot-labeled aptamers, we successfully imaged and analyzed the diffusive ...
Oh Eung Seok - - 2014
ABSTRACT. Parkinson's disease (PD) is characterized by progressive dopaminergic cell loss in the substantia nigra (SN) and elevated iron levels demonstrated by autopsy. Direct visualization of iron with live imaging techniques has not yet been successful. The aim of this study is to visualize and quantify the distribution of cellular ...
Hong Yoochan - - 2014
ABSTRACT. In this study, we describe the development of a cancer biomarker-sensitive nanobiosensor based on localized surface plasmon resonance that enables recognition for proteolytic activity of membrane type 1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells. First of all, we prepared biomarker-detectable substrate based on gold nanorods (GNRs) using ...
Choi Jihye - - 2014
ABSTRACT. Biomarker-specific photothermal nanoparticles that can efficiently sense markers that are overexpressed in distinguished adenocarcinomas have attracted much interest in an aspect of efficacy increase of cancer treatment. We demonstrated a promising prospect of a smart photothermal therapy agent employing anti-epidermal growth factor receptor aptamer (AptEGFR)-conjugated polyethylene glycol (PEG) layted ...
Kim Chang-Beom - - 2014
ABSTRACT. Alzheimer's disease (AD) is a neurodegenerative disorder that leads to a decline in cognitive and intellectual abilities and an irreversible mental deterioration. Based on multidisciplinary AD research, the most universally accepted hypotheses on AD pathogenesis are the intracerebral aggregate formation of beta-amyloid (Aβ) peptides. According to medical paradigmatic transition ...
Zhou Peifu - - 2014
The transcriptional factor plays an important role in the regulation of gene expression and crucial to understand the biology of organisms. Mycobacterium tuberculosis, the causative agent of tuberculosis, harbors multiple transcriptional factors. Few transcriptional factors are well-documented antigens. Based on the screening of antigen with TB patients sera, a functional ...
Alzoman Nourah Z - - 2014
This article describes, for the first time, a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) for therapeutic monitoring and pharmacokinetic studies of lenalidomide (LND), the potent drug for treatment of multiple myeloma (MM). The assay employed a polyclonal antibody that specifically recognizes LND with high affinity, and LND conjugate ...
Zhou Jianwei - - 2014
To date, the complete mechanism of rheumatoid arthritis (RA) remain unclear, T cells have been proposed to play an important role in the disease initiation and progression. Presently, some researchers have reported that there were skewed TCR Vβ in different samples of experimental animals or RA patients, such as in ...
Kumari G Lakshmi - - 2014
In steroid immunoassays, selection of right combination of antibody and enzyme-labeled antigen determine the sensitivity and specificity of ELISA. Antibodies raised against different positions of progesterone adopting heterologous systems were reported to provide better assays for progesterone. Four different antibodies developed against progesterone-11α-hemiglutarate-BSA (P-11-HG-BSA), progesterone-11α-hemisuccinate-BSA (P-11-HS-BSA), progesterone-3-O-carboxymethyloxime-BSA (P-3-CMO-BSA), and progesterone-3-O-carboxymethyloxime-ovalbumin ...
Kumari G Lakshmi - - 2014
Enzyme-linked immunosorbent assays (ELISA's) reported for thyroxine (T4) and 3,5,3'-triiodothyronine (T3), involved coupling of the haptens through (i) carboxylic group to carrier protein for producing antibodies and (ii) amino group to detection labels. To improve the titer and specificity of antibodies, immunogens were prepared by coupling of carboxyl group to ...
Mu Alan Kang-Wai - - 2014
The main purpose of this article is to develop a new and reliable saliva-based clinical diagnostic method for the early detection of oral squamous cell carcinoma (OSCC). This study used an immunoproteomic approach which allowed the detection of immunogenic host proteins in patients' samples using pooled human antibodies. In an ...
Ashraf Sajjad - - 2014
To overcome and eliminate tuberculosis (TB), definitive, reliable, and rapid diagnosis is mandatory. Presently, the diagnostic potential of acute and latent stage TB specific antigens i.e., Rv3803c and Rv2626c was determined. Immunogenic recombinant genes of Rv3803c and Rv2626c antigens were cloned in bacterial expression vector pET23b and expressed product was ...
Pata Supansa - - 2014
Couples in which both partners carry the α-thalassemia-1 trait have a 25% risk of hemoglobin Bart's hydrops fetalis in each pregnancy. Identification of α-thalassemia-1 trait is, therefore, necessary in order to control this severe form of α-thalassemia. We have generated monoclonal antibodies specific to the ζ-globin chain without cross reaction ...
Thattiyaphong Aree - - 2014
The selling and importing of puffer fish species and their products was banned in Thailand in 2002, because of possible neurotoxic effects. However, the sale of their flesh is still happening in Thai markets. Standard methods for toxin quantification (HPLC and LC-MS) have significant limitations, therefore a lateral flow, immuno-chromatographic ...
Tran Kalvin Q KQ Department of Molecular and Cell Biology and The Cancer Research Laboratory, University of California at Berkeley, Berkeley, California, - - 2014
Relatively little is known about the antiproliferative effects of artemisinin, a naturally occurring antimalarial compound from Artemisia annua, or sweet wormwood, in human endometrial cancer cells. Artemisinin induced a G1 cell cycle arrest in cultured human Ishikawa endometrial cancer cells and downregulated cyclin-dependent kinase-2 (CDK2) and CDK4 transcript and protein ...
Sipahi Ilke I Department of Cardiology, Acibadem University Medical School, Istanbul, Turkey2Harrington Heart and Vascular Institute, University Hospitals Case Medical Center, Case Western Reserve University School of Medicine, Cleveland, - - 2014
Recent trials of percutaneous coronary intervention (PCI) vs coronary artery bypass grafting (CABG) for multivessel disease were not designed to detect a difference in mortality and therefore were underpowered for this outcome. Consequently, the comparative effects of these 2 revascularization methods on long-term mortality are still unclear. In the absence ...
Weder Walter - - 2014
Carinal resection and sleeve pneumonectomy are rare procedures and challenging issues in thoracic surgery. In spite of the knowledge of the technique, the incidence of postoperative complications is higher compared with standard resections. Adequate patient selection, improved anesthetic management and surgical technique, and better postoperative management might reduce the rate ...
Hecker Erich - - 2014
There is no universally valid definition of the extent of tracheal resections that would be considered "extended." Underlying disease, necessary length of resection, anatomic localization, and chosen surgical approach account for a manifold interdependency. Existing data suggest a "cutoff margin" of 4 cm or more, referring to the likelihood of complications ...
Welter Stefan - - 2014
Tracheobronchial injuries (TBIs) are caused by blunt, penetrating injury or by iatrogenic damage. Most injuries are life threatening and need early and skillful airway management. Bronchoscopy remains the gold standard of diagnosis. Penetrating TBI always needs blunt trauma, and iatrogenic TBI sometimes needs surgical exploration and reconstruction, which is performed ...
Kugler Christian - - 2014
Tracheomalacia is excessive collapsibility of the trachea, typically during expiration. Congenital forms are associated with severe symptoms. Milder forms often present after the neonatal period. Adult malacia is mostly associated with chronic obstructive pulmonary disease. Functional bronchoscopy is still not standardized. Dynamic airway CT is a promising tool for noninvasive ...
Theunissen Stephanie C P M SC Department of Otorhinolaryngology and Head and Neck Surgery, Leiden University Medical Center, Leiden, the - - 2014
Pediatric hearing impairment is a chronic handicap that can potentially lead to the development of psychopathology. Yet, for hearing-impaired children and adolescents, the exact occurrence of various forms of psychopathology and its causes are unclear, while this knowledge is essential to enable targeted screenings and interventions. To investigate the level ...
Wiedemann Klaus - - 2014
Tracheobronchial surgery constitutes a challenge to the anesthetist because it involves the anatomic structures dedicated to bulk gas transport. Common approaches to airway management and gas exchange for extrathoracic and intrathoracic airway surgery are reviewed, with due regard to less common methods thought crucial for specific procedures. Tracheal surgery, beyond ...
Junker Klaus - - 2014
Malignant involvement of the trachea predominantly results from direct spread of neighboring tumors to the tracheal wall. Primary tracheal malignancies show a low incidence of approximately 0.1 in every 100,000 persons per year, squamous cell carcinomas and adenoid cystic carcinomas accounting for about two-thirds of adult primary tracheal tumors. The ...
Jungebluth Philipp - - 2014
No definitive solution has been discovered for replacing long segments or the entire trachea in humans. Most of this challenge stems from the specific function and mechanics that are almost impossible to replicate except in the setting of an allotransplantation, which requires lifelong immunosuppressive medication. Recently, tissue engineering provided significant ...
Stoelben Erich - - 2014
Benign stenosis of trachea results mainly from tracheotomy, ventilation, or trauma. The combination of a defect of the mucosa or the tracheal wall and infection produce secondary scar tissue healing with shrinkage of the tracheal lumen or instability of the tracheal wall. Standard of treatment consists of resection of the ...
Freitag Lutz - - 2014
For all cases of tracheal obstructions surgery should be considered first. Interventional endoscopic procedures can provide immediate relief. Intraluminally growing tumors can be resected with laser, argon-plasma coagulation, an electrosurgical knife or cryo-probe. Photodynamic therapy of smaller tracheal tumors can be curative. Narrowing from intramural tumor growth or wall destruction ...
Leschber Gunda - - 2014
Complications in tracheal surgery are not uncommon but generally do not influence the final result. Most patients benefit from tracheal resections and mortality is low. Risk factors for complications are reoperations, preoperative tracheostomy, and lengthy resections. Precise information about the extent of the diseased tracheal part as well as thorough ...
Olds David L DL Department of Pediatrics, School of Medicine, University of Colorado Denver, - - 2014
The Nurse-Family Partnership delivered by nurses has been found to produce long-term effects on maternal and child health in replicated randomized trials. A persistent question is whether paraprofessional home visitors might produce comparable effects. To examine the impact of prenatal and infancy/toddler home visits by paraprofessionals and by nurses on ...
Ota Naotoshi - - 2014
We compared the efficacies of ¹⁸F-fluoride positron emission tomography (¹⁸F-fluoride PET)/CT, ¹⁸F-fludeoxyglucose PET (¹⁸F-FDG PET)/CT, and ⁹⁹mTc bone scintigraphy [planar and single photon emission CT (SPECT)] for the detection of bone metastases in patients with differentiated thyroid carcinoma (DTC). We examined 11 patients (8 females and 3 males; mean age ...
Zeeshan Ahmad - - 2014
Patients with tracheal stenosis can experience life-threatening dyspnea. When the stenosis is close to the larynx, management can be challenging. Non-definitive palliative measures should be kept to a minimum as they can make definitive management more complicated. Specialized techniques of resection and reconstruction of high tracheal lesions are available and ...
Behringer Dirk - - 2014
A patient identified with tracheal cancer benefits most from evaluation by an experienced center and an extensive effort to assess the possibility of a complete surgical resection as the most efficient treatment option for cure. Localized, nonoperable disease may still be controlled by combined modality using chemotherapy and concurrent radiation.
Stergiopoulos Kathleen K Division of Cardiovascular Medicine, Department of Medicine, State University of New York-Stony Brook School of Medicine, Stony - - 2014
Myocardial ischemia in patients with stable coronary artery disease (CAD) has been repeatedly associated with impaired survival. However, it is unclear if revascularization with percutaneous coronary intervention (PCI) to relieve ischemia improves outcomes compared with medical therapy (MT). The objective of this study was to compare the effect of PCI ...
Jang Sung-Soo - - 2014
For many years, brain ischemia has been known to be a leading cause of adult neurological disorder. In particular, many reports have shown that hyperexcitability of neurons and inflammatory response of the glia induced by ischemic reperfusion (I/R) determine the fate of cells in the ischemic core and the penumbra ...
Schroeder Stephen M - - 2014
Perioperative management of diabetic patients involves optimizing glycemic control and negotiating comorbidities to help reduce complications and obtain results on par with nondiabetics. These goals are usually achievable in the elective surgical setting, but they can be more difficult to control in urgent or emergent situations. Understanding and recognizing the ...
Min Martin Renqiang - - 2014
Disrupted or abnormal biological processes responsible for cancers often quantitatively manifest as disrupted additive and multiplicative interactions of gene/protein expressions correlating with cancer progression. However, the examination of all possible combinatorial interactions between gene features in most case-control studies with limited training data is computationally infeasible. In this paper, we ...
Blume Peter A - - 2014
This article reviews current literature discussing the etiology, pathophysiology, diagnosis and imaging, and conservative and surgical treatment of Charcot osteoarthropathy. The treatment of Charcot osteoarthropathy with concurrent osteomyelitis is also discussed.
Liu Yashu - - 2014
Complex diseases such as major depression affect people over time in complicated patterns. Longitudinal data analysis is thus crucial for understanding and prognosis of such diseases and has received considerable attention in the biomedical research community. Traditional classification and regression methods have been commonly applied in a simple (controlled) clinical ...
Fliedl Lukas - - 2014
Transient gene expression (TGE) has become a powerful tool over the last decade, as time to market is a key factor in biopharmaceutical industry. TGE is used to produce small amounts of recombinant protein for functional and structural studies. To broaden the applicability of the method, the scale-up of TGE ...
Hall Molly A - - 2014
Environment-wide association studies (EWAS) provide a way to uncover the environmental mechanisms involved in complex traits in a high-throughput manner. Genome-wide association studies have led to the discovery of genetic variants associated with many common diseases but do not take into account the environmental component of complex phenotypes. This EWAS ...
Vembu Shankar - - 2014
Label propagation methods are extremely well-suited for a variety of biomedical prediction tasks based on network data. However, these algorithms cannot be used to integrate feature-based data sources with networks. We propose an efficient learning algorithm to integrate these two types of heterogeneous data sources to perform binary prediction tasks ...
Stacey Glyn N - - 2014
The history of the culture of animal cell lines is littered with published and much unpublished experience with cell lines that have become switched, mislabelled, or cross-contaminated during laboratory handling. To deliver valid and good quality research and to avoid waste of time and resources on such rogue lines, it ...
Oetjens Matthew - - 2014
Calcineurin-inhibitors CI are immunosuppressive agents prescribed to patients after solid organ transplant to prevent rejection. Although these drugs have been transformative for allograft survival, long-term use is complicated by side effects including nephrotoxicity. Given the narrow therapeutic index of CI, therapeutic drug monitoring is used to prevent acute rejection from ...
Mayba Oleg - - 2014
Cancer cells derived from different stages of tumor progression may exhibit distinct biological properties, as exemplified by the paired lung cancer cell lines H1993 and H2073. While H1993 was derived from chemo-naive metastasized tumor, H2073 originated from the chemo-resistant primary tumor from the same patient and exhibits strikingly different drug ...
Tyler Anna L - - 2014
The following sections are included: Introduction Defining Genetic Elements Defining Phenotypes The Prevalence of Pleiotropy Pleiotropy in an Evolutionary Context Pleiotropy and Human Health Session Contributions Acknowledgments References.
Williams Sarah R - - 2014
This article reviews important literature on the FAST and E-FAST examinations in adults. It also reviews key pitfalls, limitations, and controversies. A practical "how-to" guide is presented. Lastly, new frontiers are explored.
Armitage Bruce A - - 2014
The guanine quadruplex is a secondary structure formed by DNA and RNA that has been implicated in regulation of gene expression and maintenance of genome stability. Guanine-rich PNA oligomers can invade DNA or RNA quadruplex targets to form heteroquadruplex structures. Affinities in the low nanomolar range are routinely observed, making ...
Grein Tanja A - - 2014
Industrial manufacturing of cell culture-derived viruses or virus-like particles for gene therapy or vaccine production are complex multistep processes. In addition to the bioreactor, such processes require a multitude of downstream unit operations for product separation, concentration, or purification. Similarly, before a biopharmaceutical product can enter the market, removal or ...
Boffeli Troy J - - 2014
Lower extremity infections are a common yet unfortunate complication of diabetes-related ulcers often requiring surgical intervention. The main goals of surgical treatment consist of selecting the appropriate procedure to effectively eradicate nonsalvageable tissue, achieve primary healing, and maximize subsequent dynamic functionality. An overview of each partial foot amputation procedure is ...
Han Henry - - 2014
Mass spectrometry based proteomics technologies have allowed for a great progress in identifying disease biomarkers for clinical diagnosis and prognosis. However, they face acute challenges from a data reproducibility standpoint, in that no two independent studies have been found to produce the same proteomic patterns. Such reproducibility issues cause the ...
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