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Lee Wong Cheng - - 2014
The development of mammalian cell cycle synchronization techniques has greatly advanced our understanding of many cellular regulatory events and mechanisms specific to different phases of the cell cycle. In this chapter, we describe a high-throughput microfluidic-based approach for cell cycle synchronization. By exploiting the relationship between cell size and its ...
Bjermer Leif - - 2014
This review provides an update on the importance of the peripheral 'small' airways in asthma. As the small airways account for less than 10% of total airway resistance, thus having little impact on standard lung function measures such as forced expiratory volume and peak flow, they have been referred to ...
Genzel Yvonne - - 2014
The production of viral vaccines in cell culture can be accomplished with primary, diploid, or continuous (transformed) cell lines. Each cell line, each virus type, and each vaccine preparation require the specific design of upstream and downstream processing. Media have to be selected as well as production vessels, cultivation conditions, ...
Ng Clara - - 2014
The emergence of multi-drug and extensive drug resistance of microbes to antibiotics poses a great threat to human health. Although drug repurposing is a promising solution for accelerating the drug development process, its application to anti-infectious drug discovery is limited by the scope of existing phenotype-, ligand-, or target-based methods. ...
Feldman Greg - - 2014
Distress tolerance (DT) is a proposed transdiagnostic factor in psychopathology, yet sources of individual differences in DT are largely unknown. The present study examined mindfulness and rumination facets as predictors of persistence on a standardized DT task (mirror tracing). Acting with awareness (a facet of mindfulness) and reflection (a potentially ...
George Melissa W MW Department of Psychology, University of South Carolina, - - 2014
The prevalence of unmet health and mental health needs among youth has spurred the growing consensus to develop strategies that integrate services to promote overall well-being. This pilot study reports on the feasibility and outcomes of a theory-driven, family-focused, integrated health-mental health promotion program for underserved adolescents receiving school mental ...
Gorska Katarzyna - - 2014
Reactions templated by a specific nucleic acid sequence have emerged as an attractive strategy for nucleic acid sensing. The Staudinger reaction using an azide-quenched fluorophore and a phosphine is particularly well suited by virtue of its bioorthogonality and biocompatibility. The reaction is promoted by a complementary nucleic acid that aligns ...
Rodrigues Maria Elisa - - 2014
Microcarrier technology opened new perspectives for anchorage-dependent cell culture, by providing increased surface areas for cell adhesion and proliferation, and therefore improving both cell and product yields obtained in these cultures. The establishment of a successful microcarrier culture depends on many factors, such as the type of microcarrier, the cells, ...
Athanasiou Athanasios - - 2014
Anchoring platysma muscle on fixed points of the face is not new. Revolutionary high superficial muscular aponeurotic system techniques and a thorough knowledge of the anatomy of the tympanomastoid fascia give us a stabile anatomic point to fixate platysma muscle. This structure combined with supraplatysmal or subplatysmal defatting, partial resection ...
Pattin Kristine A - - 2014
Many colleges and universities across the globe now offer bachelors, masters, and doctoral degrees, along with certificate programs in bioinformatics. While there is some consensus surrounding curricula competencies, programs vary greatly in their core foci, with some leaning heavily toward the biological sciences and others toward quantitative areas. This allows ...
Costa Ana Rita - - 2014
Fed-batch processes are a current preference for the production of recombinant proteins in mammalian cells. The use of nutrient feeding prevents the depletion of important medium components and results in improved culture longevity and high cell and product yields. To take maximum advantage of these effects, it is important to ...
Babitzky Alexander - - 2014
This protocol regards a microscopic application and software for image-guided monitoring of mammalian cells which grow in suspension cultures. It has been developed in order to establish an automated microscopic application for in situ and at-line cell monitoring in bioreactors (Akin et al., Biosens Bioelectron 26:4532-4537, 2011; Babitzky et al., ...
Ristow Brunno - - 2014
This article addresses specific questions posed to the author by the editor to elucidate his 40-years experience with neck lift. Surgery of the neck has been, in the author's hands, for all practical purpose, problem free. However, one needs to understand the issues and apply the proper techniques. By far, ...
Paek Hye-Jin - - 2014
This study evaluated a community-based and social marketing healthy corner store program (FIT store) to improve the affordability and availability of healthy foods in low-income, urban, and ethnically diverse neighborhoods in Michigan. The Nutrition Environment Measures Survey in Stores data were analyzed for the FIT (N = 4) stores. Two ...
Zengeya Thomas - - 2014
Noncoding RNAs are attractive targets for molecular recognition because of the central role they play in gene expression. Since most noncoding RNAs are in a double-helical conformation, recognition of such structures is a formidable problem. Herein, we describe a method for sequence-selective recognition of biologically relevant double-helical RNA (illustrated on ...
Aranibar Nelly - - 2014
Metabolomics has become an important tool for measuring pools of small molecules in mammalian cell cultures expressing therapeutic proteins. NMR spectroscopy has played an important role, largely because it requires minimal sample preparation, does not require chromatographic separation, and is quantitative. The concentrations of large numbers of small molecules in ...
Luz Dilson - - 2014
The author describes 13 years of experience in the application of a new and simple method of facial cutaneous detachment using specially designed bifaceted rigid steel wands called Dilson Luz Vascular Dilation Wands. This technique, termed progressive tunnelizations, involves stretching the blood vessels to the point of rupture by inserting progressively ...
Cheng Chao - - 2014
The human genome encodes a large number of non-coding RNAs, which employ a new and crucial layer of biological regulation in addition to proteins. Technical advancement in recent years, particularly, the wide application of next generation sequencing analysis, provide an unprecedented opportunity to identify new non-coding RNAs and investigate their ...
Druzinec Damir - - 2014
Modern bioprocesses demand for a careful definition of the critical process parameters (CPPs) already during the early stages of process development in order to ensure high-quality products and satisfactory yields. In this context, online monitoring tools can be applied to recognize unfavorable changes of CPPs during the production processes and ...
Morgan Alexander A - - 2014
We propose and discuss a method for doing gene expression meta-analysis (multiple datasets) across multiplex measurement modalities measuring the expression of many genes simultaneously (e.g. microarrays and RNAseq) using external control samples and a method of heterogeneity detection to identify and filter on comparable gene expression measurements. We demonstrate this ...
Nelson Ryan R *Section of Gastroenterology, Hepatology, and Nutrition, †Department of Health Studies, the University of Chicago Medicine, Chicago, Illinois; and ‡Department of Surgery, University of Washington Medicine, Seattle, - - 2014
Cyclosporine and infliximab (IFX) are effective medical therapies for inducing remission in patients with steroid-refractory ulcerative colitis (UC). Patients with acute severe disease who do not respond to these therapies require colectomy, however, the risk of postoperative complications in such patients is not known. Analyzing patients with acute severe UC, ...
Gonzalez Raul - - 2014
Lateral plication of the platysma can substitute medial plication in all cases, no matter the severity of the case, with several advantages. This article presents a useful method, called lateral overlapping plication of the platysma (LOPP). In addition to the simplicity of the procedure, it has other advantages over medial ...
Arredondo Elva M - - 2014
Physical activity (PA) rates in young Latina girls are low. This study examined acceptability and feasibility of implementing a mother-daughter intervention targeting individual and family-level correlates of PA. Eleven mother-daughter dyads participated in an 8-week intervention promoting PA in preadolescents. Preliminary data suggest increases in self-report PA, reductions in television ...
Xiao Zhihua - - 2014
The majority of therapeutic proteins are expressed in mammalian cells, predominantly in Chinese Hamster Ovary cells. While cell culture media and feed supplements are crucial to protein productivity, medium optimization can be labor intensive and time-consuming. In this chapter, we describe some basic concepts in medium development and introduce a ...
Compalati Enrico - - 2014
The aim is to review recent literature up to July 2013 concerning the effect of allergen-specific immunotherapy (AIT) on asthma. AIT, effective in combined allergic rhinitis and asthma, was previously described as a convenient approach able to improve clinical outcomes and reduce bronchial hyperresponsiveness. In addition, long-term and preventive effects ...
Enriquez J Luis - - 2014
The use of ultrasonography in medical practice has evolved dramatically over the last few decades and will continue to improve as technological advances are incorporated into daily medical practice. Although ultrasound machine size and equipment have evolved, the basic principles and fundamental functions have remained essentially the same. This article ...
Cremer Heike - - 2014
The use of baculoviruses has become a standard approach in many labs for recombinant protein production. In addition to giving a broad and practical overview of the technology, this chapter focuses in particular on two recent developments in the field and how these can be efficiently exploited for protein production: ...
Brunak Søren - - 2014
Precision medicine promises to transform cancer treatment in the next decade through the use of high-throughput sequencing and other technologies to identify telltale molecular aberrations that reveal therapeutic vulnerabilities of each patient's tumor [1]. This session will address the "panomics" of cancer - the complex combination of patient-specific characteristics that ...
Winssinger Nicolas - - 2014
The biochemical stability and desirable hybridization properties of peptide nucleic acids (PNA) coupled to the robustness of the peptidic chemistry involved in their oligomerization make them an attractive nucleic acid tag to encode molecules and program their assembly into higher order oligomers. The ability to program the dimerization of ligands ...
Duarte Tiago M - - 2014
(1)H-Nuclear magnetic resonance ((1)H-NMR) spectroscopy is a powerful technique to analyze the composition of complex mixtures based on the particular proton fingerprint of each molecule. Here we describe a protocol for exometabolome analysis of mammalian cells using this technique, including sample preparation, spectra acquisition, and integration. The potential of this ...
Smolina Irina V - - 2014
The unique ability of triplex-forming PNAs to invade the double helix has made it possible to develop a highly specific and sensitive approach for bacterial detection. The method uses short, about 20-bp-long, signature sequences presented as a single copy in the bacterial genome. Bacterial cells are fixed on slides and ...
Jang In Sock - - 2014
Large-scale pharmacogenomic screens of cancer cell lines have emerged as an attractive pre-clinical system for identifying tumor genetic subtypes with selective sensitivity to targeted therapeutic strategies. Application of modern machine learning approaches to pharmacogenomic datasets have demonstrated the ability to infer genomic predictors of compound sensitivity. Such modeling approaches entail ...
Badea Liviu - - 2014
We present a joint analysis method for mutation and gene expression data employing information about proteins that are highly interconnected at the level of protein to protein (pp) interactions, which we apply to the TCGA Acute Myeloid Leukemia (AML) dataset. Given the low incidence of most mutations in virtually all ...
Darabos Christian - - 2014
With the rapid increase in the quality and quantity of data generated by modern high-throughput sequencing techniques, there has been a need for innovative methods able to convert this tremendous amount of data into more accessible forms. Networks have been a corner stone of this movement, as they are an ...
Ito Kenichiro - - 2014
Site-selective scission of genomes is quite important for future biotechnology. However, naturally occurring restriction enzymes cut these huge DNAs at too many sites and cannot be used for this purpose. Recently, we have developed a completely chemistry-based artificial restriction DNA cutter (ARCUT) by combining a pair of pseudo-complementary PNA (pcPNA) ...
Listgarten Jennifer - - 2014
The following sections are included: Session contributions References.
Sagray Bryan A - - 2014
Treatment of the patient with a diabetic foot infection and underlying osteomyelitis is currently an evolving process, often complicated by neuropathy, peripheral vascular disease, and renal insufficiency. Understanding which patients require hospitalization, intravenous antibiotic therapy, and urgent operative intervention may ultimately prevent the spread of infection or major limb amputation. ...
Shriki Jesse - - 2014
Bedside ultrasound has become an important modality for obtaining critical information in the acute care of patients. It is important to understand the physics of ultrasound in order to perform and interpret images at the bedside. The physics of both continuous wave and pulsed wave sound underlies diagnostic ultrasound. The ...
Sams Michael M Upper Austria University of Applied Sciences, Campus Linz, Garnisonstrasse 21, 4020 Linz, - - 2014
We present a cluster spatial analysis method using nanoscopic dSTORM images to determine changes in protein cluster distributions within brain tissue. Such methods are suitable to investigate human brain tissue and will help to achieve a deeper understanding of brain disease along with aiding drug development. Human brain tissue samples ...
Gonzalez Graciela - - 2014
Text and data mining methods constantly advance and are applied in different fields. In order for them to impact the biomedical discovery process, it is necessary to thoroughly engage scientists at both ends, and conduct thorough empirical evaluations as to their ability to suggest novel hypotheses and address the most ...
Shiraishi Takehiko - - 2014
Cellular delivery methods are a prerequisite for cellular studies with PNA. This chapter describes PNA cellular delivery using cell-penetrating peptide (CPP)-PNA conjugates and transfection of PNA-ligand conjugates mediated by cationic lipids. Furthermore, two endosomolytic procedures employing chloroquine treatment or photochemical internalization (PCI) for significantly improving PNA delivery efficacy are described.
Roloff Alexander - - 2014
Single base-specific detection of DNA/RNA sequences is of importance in the diagnosis of disease-associated genetic disorders or early stage cancer. This chapter introduces DNA-templated native chemical PNA ligation as a potentially useful tool for the sequence specific detection of nucleic acids. The template-induced alignment of PNA-thioesters and 1,2-aminothiol-PNAs in close ...
Jordan Martin - - 2014
Shaking technology in combination with small-scale disposable plastic vessels has become a notable bioprocess optimization tool widely exploited for cells grown in suspension. This chapter focuses on the two most accommodating culture systems: 50 mL centrifugation tubes and 96-deepwell plates. Used by many laboratories for routine passaging of suspension cultures ...
De Castro Claudio Cardoso - - 2014
The authors deal with different kinds of patients exhibiting different anatomic arrangements of the structures in their neck, different ages, and different expectations; there are several techniques from which to choose. Patients have no idea of the deformity they show. They demand a fast recovery with the best possible result ...
Pendergrass Sarah - - 2014
A clear and predictive understanding of the etiology of autism spectrum disorders (ASD), a group of neurodevelopmental disorders characterized by varying deficits in social interaction and communication as well as repetitive behaviors, has not yet been achieved. There remains active debate about the origins of autism, and the degree to ...
Frahm Björn - - 2014
For the production of biopharmaceuticals a seed train is required to generate an adequate number of cells for inoculation of the production bioreactor. This seed train is time- and cost-intensive but offers potential for optimization. A method and a protocol are described for the seed train mapping, directed modeling without ...
Desantis Stacia M - - 2014
Determining the effectiveness of different treatments from observational data, which are characterized by imbalance between groups due to lack of randomization, is challenging. Propensity matching is often used to rectify imbalances among prognostic variables. However, there are no guidelines on how appropriately to analyze group matched data when the outcome ...
Shalaby Sherif Y - - 2014
The diabetic population is increasing worldwide at a staggering rate. Diabetic foot ulcers are a major contributor to nontraumatic lower limb amputations and peripheral arterial disease is one of main contributing pathophysiologic causes of diabetic ulcers. The dire need to reduce complication and wound healing recovery period of the chronic ...
Perina Alessandro - - 2014
The immune system gathers evidence of the execution of various molecular processes, both foreign and the cells' own, as time- and space-varying sets of epitopes, small linear or conformational segments of the proteins involved in these processes. Epitopes do not have any obvious ordering in this scheme: The immune system ...
Blucher Aurora S - - 2014
Repurposing an existing drug for an alternative use is not only a cost effective method of development, but also a faster process due to the drug's previous clinical testing and established pharmokinetic profiles. A potentially rich resource for computational drug repositioning approaches is publically available high throughput screening data, available ...
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